1xcf - Revision history

OCA at 08:06, 25 October 2023

OCA — Wed, 25 Oct 2023 08:06:29 GMT

←Older revision		Revision as of 08:06, 25 October 2023
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	<StructureSection load='1xcf' size='340' side='right'caption='[[1xcf]], [[Resolution\|resolution]] 1.80Å' scene=''>		<StructureSection load='1xcf' size='340' side='right'caption='[[1xcf]], [[Resolution\|resolution]] 1.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1xcf]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~"bacillus_coli"_migula_1895 "bacillus~~ coli~~" migula 1895~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XCF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1XCF FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1xcf]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XCF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1XCF FirstGlance]. <br>
-	</td></tr><tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat">~~<div style='overflow: auto; max~~-~~height: 3em;'>[[1k8x\|1k8x]]~~, [[~~1xc4~~\|~~1xc4~~]]~~</div></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.8Å</td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Tryptophan_synthase Tryptophan synthase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.~~20 4.2.1.20] </span>~~</td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1xcf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1xcf OCA], [https://pdbe.org/1xcf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1xcf RCSB], [https://www.ebi.ac.uk/pdbsum/1xcf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1xcf ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1xcf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1xcf OCA], [https://pdbe.org/1xcf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1xcf RCSB], [https://www.ebi.ac.uk/pdbsum/1xcf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1xcf ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/TRPA_ECOLI TRPA_ECOLI]] The alpha subunit is responsible for the aldol cleavage of indoleglycerol phosphate to indole and glyceraldehyde 3-phosphate.	+	[https://www.uniprot.org/uniprot/TRPA_ECOLI TRPA_ECOLI] The alpha subunit is responsible for the aldol cleavage of indoleglycerol phosphate to indole and glyceraldehyde 3-phosphate.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Bacillus~~ coli ~~migula 1895~~]]	+	[[Category: Escherichia coli]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	~~[[Category: Tryptophan synthase]]~~	+	[[Category: Jang SB]]
-	[[Category: Jang~~, S B]]~~	+
-	~~[[Category: A-subunit]]~~	+
-	~~[[Category: E coli]]~~	+
-	~~[[Category: Lyase]]~~	+
-	~~[[Category: P28l/y173f double mutant~~]]	+

OCA at 16:36, 27 October 2021

OCA — Wed, 27 Oct 2021 16:36:14 GMT

←Older revision		Revision as of 16:36, 27 October 2021
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	<StructureSection load='1xcf' size='340' side='right'caption='[[1xcf]], [[Resolution\|resolution]] 1.80Å' scene=''>		<StructureSection load='1xcf' size='340' side='right'caption='[[1xcf]], [[Resolution\|resolution]] 1.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1xcf]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XCF OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1XCF FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1xcf]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XCF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1XCF FirstGlance]. <br>
-	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1k8x\|1k8x]], [[1xc4\|1xc4]]</td></tr>	+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1k8x\|1k8x]], [[1xc4\|1xc4]]</div></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Tryptophan_synthase Tryptophan synthase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.20 4.2.1.20] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Tryptophan_synthase Tryptophan synthase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.20 4.2.1.20] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1xcf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1xcf OCA], [~~http~~://pdbe.org/1xcf PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1xcf RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1xcf PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1xcf ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1xcf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1xcf OCA], [https://pdbe.org/1xcf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1xcf RCSB], [https://www.ebi.ac.uk/pdbsum/1xcf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1xcf ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/TRPA_ECOLI TRPA_ECOLI]] The alpha subunit is responsible for the aldol cleavage of indoleglycerol phosphate to indole and glyceraldehyde 3-phosphate.	+	[[https://www.uniprot.org/uniprot/TRPA_ECOLI TRPA_ECOLI]] The alpha subunit is responsible for the aldol cleavage of indoleglycerol phosphate to indole and glyceraldehyde 3-phosphate.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	==See Also==		==See Also==
-	*[[Tryptophan synthase\|Tryptophan synthase]]	+	*[[Tryptophan synthase 3D structures\|Tryptophan synthase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 17:47, 22 January 2020

OCA — Wed, 22 Jan 2020 17:47:51 GMT

←Older revision		Revision as of 17:47, 22 January 2020
Line 1:		Line 1:

	==Crystal structure of P28L/Y173F tryptophan synthase alpha-subunits from Escherichia coli==		==Crystal structure of P28L/Y173F tryptophan synthase alpha-subunits from Escherichia coli==
-	<StructureSection load='1xcf' size='340' side='right' caption='[[1xcf]], [[Resolution\|resolution]] 1.80Å' scene=''>	+	<StructureSection load='1xcf' size='340' side='right'caption='[[1xcf]], [[Resolution\|resolution]] 1.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1xcf]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XCF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1XCF FirstGlance]. <br>		<table><tr><td colspan='2'>[[1xcf]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XCF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1XCF FirstGlance]. <br>
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	</StructureSection>		</StructureSection>
	[[Category: Bacillus coli migula 1895]]		[[Category: Bacillus coli migula 1895]]
		+	[[Category: Large Structures]]
	[[Category: Tryptophan synthase]]		[[Category: Tryptophan synthase]]
	[[Category: Jang, S B]]		[[Category: Jang, S B]]

OCA at 07:26, 18 April 2018

OCA — Wed, 18 Apr 2018 07:26:53 GMT

←Older revision		Revision as of 07:26, 18 April 2018
Line 1:		Line 1:
		+
	==Crystal structure of P28L/Y173F tryptophan synthase alpha-subunits from Escherichia coli==		==Crystal structure of P28L/Y173F tryptophan synthase alpha-subunits from Escherichia coli==
	<StructureSection load='1xcf' size='340' side='right' caption='[[1xcf]], [[Resolution\|resolution]] 1.80Å' scene=''>		<StructureSection load='1xcf' size='340' side='right' caption='[[1xcf]], [[Resolution\|resolution]] 1.80Å' scene=''>
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	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1k8x\|1k8x]], [[1xc4\|1xc4]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1k8x\|1k8x]], [[1xc4\|1xc4]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Tryptophan_synthase Tryptophan synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.20 4.2.1.20] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Tryptophan_synthase Tryptophan synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.20 4.2.1.20] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1xcf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1xcf OCA], [http://pdbe.org/1xcf PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1xcf RCSB], [http://www.ebi.ac.uk/pdbsum/1xcf PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1xcf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1xcf OCA], [http://pdbe.org/1xcf PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1xcf RCSB], [http://www.ebi.ac.uk/pdbsum/1xcf PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1xcf ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 13:		Line 14:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/xc/1xcf_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/xc/1xcf_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>

OCA at 08:14, 8 February 2016

OCA — Mon, 08 Feb 2016 08:14:05 GMT

←Older revision		Revision as of 08:14, 8 February 2016
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	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1xcf ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 09:36, 10 September 2015

OCA — Thu, 10 Sep 2015 09:36:15 GMT

←Older revision		Revision as of 09:36, 10 September 2015
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	<StructureSection load='1xcf' size='340' side='right' caption='[[1xcf]], [[Resolution\|resolution]] 1.80Å' scene=''>		<StructureSection load='1xcf' size='340' side='right' caption='[[1xcf]], [[Resolution\|resolution]] 1.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1xcf]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Escherichia_coli Escherichia~~ coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XCF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1XCF FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1xcf]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XCF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1XCF FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1k8x\|1k8x]], [[1xc4\|1xc4]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1k8x\|1k8x]], [[1xc4\|1xc4]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Tryptophan_synthase Tryptophan synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.20 4.2.1.20] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Tryptophan_synthase Tryptophan synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.20 4.2.1.20] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1xcf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1xcf OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1xcf RCSB], [http://www.ebi.ac.uk/pdbsum/1xcf PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1xcf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1xcf OCA], [http://pdbe.org/1xcf PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1xcf RCSB], [http://www.ebi.ac.uk/pdbsum/1xcf PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
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	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1xcf" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 34:		Line 35:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Escherichia~~ coli]]	+	[[Category: Bacillus coli migula 1895]]
	[[Category: Tryptophan synthase]]		[[Category: Tryptophan synthase]]
	[[Category: Jang, S B]]		[[Category: Jang, S B]]

OCA at 21:54, 24 December 2014

OCA — Wed, 24 Dec 2014 21:54:06 GMT

←Older revision		Revision as of 21:54, 24 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1xcf]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XCF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1XCF FirstGlance]. <br>		<table><tr><td colspan='2'>[[1xcf]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XCF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1XCF FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1k8x\|1k8x]], [[1xc4\|1xc4]]</td></tr>	+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1k8x\|1k8x]], [[1xc4\|1xc4]]</td></tr>
-	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Tryptophan_synthase Tryptophan synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.20 4.2.1.20] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Tryptophan_synthase Tryptophan synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.20 4.2.1.20] </span></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1xcf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1xcf OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1xcf RCSB], [http://www.ebi.ac.uk/pdbsum/1xcf PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1xcf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1xcf OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1xcf RCSB], [http://www.ebi.ac.uk/pdbsum/1xcf PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/TRPA_ECOLI TRPA_ECOLI]] The alpha subunit is responsible for the aldol cleavage of indoleglycerol phosphate to indole and glyceraldehyde 3-phosphate.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 34:		Line 36:
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
	[[Category: Tryptophan synthase]]		[[Category: Tryptophan synthase]]
-	[[Category: Jang, S B.]]	+	[[Category: Jang, S B]]
	[[Category: A-subunit]]		[[Category: A-subunit]]
	[[Category: E coli]]		[[Category: E coli]]
	[[Category: Lyase]]		[[Category: Lyase]]
	[[Category: P28l/y173f double mutant]]		[[Category: P28l/y173f double mutant]]
-	[[Category: Tryptophan synthase]]

OCA at 22:00, 29 September 2014

OCA — Mon, 29 Sep 2014 22:00:22 GMT

←Older revision		Revision as of 22:00, 29 September 2014
Line 1:		Line 1:
-	[[~~Image:~~1xcf.~~png~~\|~~left~~\|200px]]	+	==Crystal structure of P28L/Y173F tryptophan synthase alpha-subunits from Escherichia coli==
		+	<StructureSection load='1xcf' size='340' side='right' caption='[[1xcf]], [[Resolution\|resolution]] 1.80Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1xcf]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XCF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1XCF FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1k8x\|1k8x]], [[1xc4\|1xc4]]</td></tr>
		+	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Tryptophan_synthase Tryptophan synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.20 4.2.1.20] </span></td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1xcf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1xcf OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1xcf RCSB], [http://www.ebi.ac.uk/pdbsum/1xcf PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/xc/1xcf_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The alpha-subunit of tryptophan synthase (alphaTS) catalyzes the cleavage of indole-3-glycerol phosphate to glyceraldehyde-3-phosphate and indole, which is used to yield the amino acid tryptophan in tryptophan biosynthesis. Here, we report the first crystal structures of wild-type and double-mutant P28L/Y173F alpha-subunit of tryptophan synthase from Escherichia coli at 2.8 and 1.8A resolution, respectively. The structure of wild-type alphaTS from E. coli was similar to that of the alpha(2)beta(2) complex structure from Salmonella typhimurium. As compared with both structures, the conformational changes are mostly in the interface of alpha- and beta-subunits, and the substrate binding region. Two sulfate ions and two glycerol molecules per asymmetric unit bind with the residues in the active sites of the wild-type structure. Contrarily, double-mutant P28L/Y173F structure is highly closed at the window for the substrate binding by the conformational changes. The P28L substitution induces the exposure of hydrophobic amino acids and decreases the secondary structure that causes the aggregation. The Y173F suppresses to transfer a signal from the alpha-subunit core to the alpha-subunit surface involved in interactions with the beta-subunit and increases structural stability.

-	~~{{STRUCTURE_1xcf\| PDB=1xcf \| SCENE= }}~~	+	Structures of wild-type and P28L/Y173F tryptophan synthase alpha-subunits from Escherichia coli.,Jeong MS, Jeong JK, Lim WK, Jang SB Biochem Biophys Res Commun. 2004 Oct 29;323(4):1257-64. PMID:15451433<ref>PMID:15451433</ref>

-	~~===Crystal structure~~ of ~~P28L~~/~~Y173F tryptophan synthase alpha-subunits from Escherichia coli===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>

-	~~{{ABSTRACT_PUBMED_15451433}}~~	+	==See Also==
-		+	*[[Tryptophan synthase\|Tryptophan synthase]]
-	==~~About this Structure~~==	+	== References ==
-	[[~~1xcf~~]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XCF OCA].	+	<references/>
-		+	__TOC__
-	==~~Reference~~==	+	</StructureSection>
-	<~~ref group="xtra">PMID:015451433<~~/~~ref~~><~~ref group="xtra">PMID:012460570</ref><references group="xtra"~~/>	+
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
	[[Category: Tryptophan synthase]]		[[Category: Tryptophan synthase]]

OCA at 12:21, 23 January 2013

OCA — Wed, 23 Jan 2013 12:21:45 GMT

←Older revision		Revision as of 12:21, 23 January 2013
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	[[Image:1xcf.png\|left\|200px]]		[[Image:1xcf.png\|left\|200px]]

-	<!--
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	{{STRUCTURE_1xcf\| PDB=1xcf \| SCENE= }}		{{STRUCTURE_1xcf\| PDB=1xcf \| SCENE= }}

	===Crystal structure of P28L/Y173F tryptophan synthase alpha-subunits from Escherichia coli===		===Crystal structure of P28L/Y173F tryptophan synthase alpha-subunits from Escherichia coli===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_15451433}}, adds the Publication Abstract to the page
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	{{ABSTRACT_PUBMED_15451433}}		{{ABSTRACT_PUBMED_15451433}}

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	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~15451433~~</ref><ref group="xtra">PMID:~~12460570~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:015451433</ref><ref group="xtra">PMID:012460570</ref><references group="xtra"/>
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
	[[Category: Tryptophan synthase]]		[[Category: Tryptophan synthase]]

OCA: Protected "1xcf" [edit=sysop:move=sysop]

OCA — Tue, 15 Mar 2011 00:12:09 GMT

Protected "1xcf" [edit=sysop:move=sysop]

←Older revision

Revision as of 00:12, 15 March 2011