1xgc - Revision history

OCA at 09:29, 6 December 2023

OCA — Wed, 06 Dec 2023 09:29:38 GMT

←Older revision		Revision as of 09:29, 6 December 2023
Line 1:		Line 1:

	==ALPHA CONOTOXIN GI: 2-3;7-13 DISULFIDE BOND ISOMER, NMR, 25 STRUCTURES==		==ALPHA CONOTOXIN GI: 2-3;7-13 DISULFIDE BOND ISOMER, NMR, 25 STRUCTURES==
-	<StructureSection load='1xgc' size='340' side='right'caption='[[1xgc~~]], [[NMR_Ensembles_of_Models \| 25 NMR models~~]]' scene=''>	+	<StructureSection load='1xgc' size='340' side='right'caption='[[1xgc]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1xgc]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XGC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1XGC FirstGlance]. <br>		<table><tr><td colspan='2'>[[1xgc]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XGC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1XGC FirstGlance]. <br>
-	</td></tr><tr id='~~NonStdRes~~'><td class="sblockLbl"><b>[[~~Non-Standard_Residue~~\|~~NonStd Res~~:]]</b></td><td class="sblockDat"><scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1xgc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1xgc OCA], [https://pdbe.org/1xgc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1xgc RCSB], [https://www.ebi.ac.uk/pdbsum/1xgc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1xgc ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1xgc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1xgc OCA], [https://pdbe.org/1xgc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1xgc RCSB], [https://www.ebi.ac.uk/pdbsum/1xgc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1xgc ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/~~CA1A_CONGE CA1A_CONGE~~]] Alpha-conotoxins act on postsynaptic membranes, they bind to the nicotinic acetylcholine receptors (nAChR) and thus inhibit them. The higher affinity site for alpha-conotoxin GI is the alpha/delta site on mouse muscle-derived BC3H-1 receptor, and the other site (alpha/gamma site) on nicotinic receptors from Torpedo californica electric organ.	+	[https://www.uniprot.org/uniprot/CAIA_CONGE CAIA_CONGE]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 23:		Line 24:
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Alewood~~, P F~~]]	+	[[Category: Alewood PF]]
-	[[Category: Craik~~, D J~~]]	+	[[Category: Craik DJ]]
-	[[Category: Gehrmann, J]]	+	[[Category: Gehrmann J]]
-	~~[[Category: Alpha-conotoxin]]~~	+
-	~~[[Category: Neurotoxin]]~~	+
-	~~[[Category: Nicotinic acetylcholine recepto disulfide bond isomer~~]]	+

OCA at 16:37, 27 October 2021

OCA — Wed, 27 Oct 2021 16:37:43 GMT

←Older revision		Revision as of 16:37, 27 October 2021
Line 3:		Line 3:
	<StructureSection load='1xgc' size='340' side='right'caption='[[1xgc]], [[NMR_Ensembles_of_Models \| 25 NMR models]]' scene=''>		<StructureSection load='1xgc' size='340' side='right'caption='[[1xgc]], [[NMR_Ensembles_of_Models \| 25 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1xgc]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XGC OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1XGC FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1xgc]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XGC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1XGC FirstGlance]. <br>
	</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene></td></tr>		</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1xgc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1xgc OCA], [~~http~~://pdbe.org/1xgc PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1xgc RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1xgc PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1xgc ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1xgc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1xgc OCA], [https://pdbe.org/1xgc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1xgc RCSB], [https://www.ebi.ac.uk/pdbsum/1xgc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1xgc ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/CA1A_CONGE CA1A_CONGE]] Alpha-conotoxins act on postsynaptic membranes, they bind to the nicotinic acetylcholine receptors (nAChR) and thus inhibit them. The higher affinity site for alpha-conotoxin GI is the alpha/delta site on mouse muscle-derived BC3H-1 receptor, and the other site (alpha/gamma site) on nicotinic receptors from Torpedo californica electric organ.	+	[[https://www.uniprot.org/uniprot/CA1A_CONGE CA1A_CONGE]] Alpha-conotoxins act on postsynaptic membranes, they bind to the nicotinic acetylcholine receptors (nAChR) and thus inhibit them. The higher affinity site for alpha-conotoxin GI is the alpha/delta site on mouse muscle-derived BC3H-1 receptor, and the other site (alpha/gamma site) on nicotinic receptors from Torpedo californica electric organ.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==

OCA at 17:56, 22 January 2020

OCA — Wed, 22 Jan 2020 17:56:59 GMT

←Older revision		Revision as of 17:56, 22 January 2020
Line 1:		Line 1:

	==ALPHA CONOTOXIN GI: 2-3;7-13 DISULFIDE BOND ISOMER, NMR, 25 STRUCTURES==		==ALPHA CONOTOXIN GI: 2-3;7-13 DISULFIDE BOND ISOMER, NMR, 25 STRUCTURES==
-	<StructureSection load='1xgc' size='340' side='right' caption='[[1xgc]], [[NMR_Ensembles_of_Models \| 25 NMR models]]' scene=''>	+	<StructureSection load='1xgc' size='340' side='right'caption='[[1xgc]], [[NMR_Ensembles_of_Models \| 25 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1xgc]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XGC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1XGC FirstGlance]. <br>		<table><tr><td colspan='2'>[[1xgc]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XGC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1XGC FirstGlance]. <br>
Line 22:		Line 22:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Alewood, P F]]		[[Category: Alewood, P F]]
	[[Category: Craik, D J]]		[[Category: Craik, D J]]

OCA at 07:33, 18 April 2018

OCA — Wed, 18 Apr 2018 07:33:49 GMT

←Older revision		Revision as of 07:33, 18 April 2018
Line 1:		Line 1:
		+
	==ALPHA CONOTOXIN GI: 2-3;7-13 DISULFIDE BOND ISOMER, NMR, 25 STRUCTURES==		==ALPHA CONOTOXIN GI: 2-3;7-13 DISULFIDE BOND ISOMER, NMR, 25 STRUCTURES==
	<StructureSection load='1xgc' size='340' side='right' caption='[[1xgc]], [[NMR_Ensembles_of_Models \| 25 NMR models]]' scene=''>		<StructureSection load='1xgc' size='340' side='right' caption='[[1xgc]], [[NMR_Ensembles_of_Models \| 25 NMR models]]' scene=''>
Line 4:		Line 5:
	<table><tr><td colspan='2'>[[1xgc]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XGC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1XGC FirstGlance]. <br>		<table><tr><td colspan='2'>[[1xgc]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XGC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1XGC FirstGlance]. <br>
	</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene></td></tr>		</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1xgc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1xgc OCA], [http://pdbe.org/1xgc PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1xgc RCSB], [http://www.ebi.ac.uk/pdbsum/1xgc PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1xgc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1xgc OCA], [http://pdbe.org/1xgc PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1xgc RCSB], [http://www.ebi.ac.uk/pdbsum/1xgc PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1xgc ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==

OCA at 11:00, 11 September 2015

OCA — Fri, 11 Sep 2015 11:00:55 GMT

←Older revision		Revision as of 11:00, 11 September 2015
Line 4:		Line 4:
	<table><tr><td colspan='2'>[[1xgc]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XGC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1XGC FirstGlance]. <br>		<table><tr><td colspan='2'>[[1xgc]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XGC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1XGC FirstGlance]. <br>
	</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene></td></tr>		</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1xgc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1xgc OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1xgc RCSB], [http://www.ebi.ac.uk/pdbsum/1xgc PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1xgc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1xgc OCA], [http://pdbe.org/1xgc PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1xgc RCSB], [http://www.ebi.ac.uk/pdbsum/1xgc PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[http://www.uniprot.org/uniprot/~~CXAA_CONGE CXAA_CONGE~~]] Alpha-conotoxins act on postsynaptic membranes, they bind to the nicotinic acetylcholine receptors (nAChR) and thus inhibit them. The higher affinity site for alpha-conotoxin GI is the alpha/delta site on mouse muscle-derived BC3H-1 receptor, and the other site (alpha/gamma site) on nicotinic receptors from Torpedo californica electric organ.	+	[[http://www.uniprot.org/uniprot/CA1A_CONGE CA1A_CONGE]] Alpha-conotoxins act on postsynaptic membranes, they bind to the nicotinic acetylcholine receptors (nAChR) and thus inhibit them. The higher affinity site for alpha-conotoxin GI is the alpha/delta site on mouse muscle-derived BC3H-1 receptor, and the other site (alpha/gamma site) on nicotinic receptors from Torpedo californica electric organ.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 16:		Line 16:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1xgc" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 15:33, 25 December 2014

OCA — Thu, 25 Dec 2014 15:33:34 GMT

←Older revision

Revision as of 15:33, 25 December 2014

OCA at 07:09, 9 June 2014

OCA — Mon, 09 Jun 2014 07:09:56 GMT

←Older revision		Revision as of 07:09, 9 June 2014
Line 1:		Line 1:
-	[[~~Image:~~1xgc.~~png~~\|~~left\|200px~~]]	+	==ALPHA CONOTOXIN GI: 2-3;7-13 DISULFIDE BOND ISOMER, NMR, 25 STRUCTURES==
		+	<StructureSection load='1xgc' size='340' side='right' caption='[[1xgc]], [[NMR_Ensembles_of_Models \| 25 NMR models]]' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1xgc]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XGC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1XGC FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene></td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1xgc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1xgc OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1xgc RCSB], [http://www.ebi.ac.uk/pdbsum/1xgc PDBsum]</span></td></tr>
		+	<table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The three possible disulfide bonded isomers of alpha-conotoxin GI have been selectively synthesised and their structures determined by 1H NMR spectroscopy. alpha-Conotoxin GI derives from the venom of Conus geographus and is a useful neuropharmacological tool as it selectively binds to the nicotinic acetylcholine receptor (nAChR), a ligand-gated ion channel involved in nerve signal transmission. The peptide has the sequence ECCNPACGRHYSC-NH2, and the three disulfide bonded isomers are referred to as GI(2-7;3-13), GI(2-13;3-7) and GI(2-3;7-13). The NMR structure for the native isomer GI(2-7;3-13) is of excellent quality, with a backbone pairwise RMSD of 0.16 A for a family of 35 structures, and comprises primarily a distorted 310 helix between residues 5 to 11. The two non-native isomers exhibit multiple conformers in solution, with the major populated forms being different in structure both from each other and from the native form. Structure-activity relationships for the native GI(2-7;3-13) as well as the role of the disulfide bonds on folding and stability of the three isomers are examined. It is concluded that the disulfide bonds in alpha-conotoxin GI play a crucial part in determining both the structure and stability of the peptide. A trend for increased conformational heterogeneity was observed in the order of GI(2-7;3-13)<GI(2-13;3-7)<GI(2-3;7-13). It was found that the peptide bond joining Cys2 to Cys3 in GI(2-3;7-13) is predominantly trans, rather than cis as theoretically predicted. These structural data are used to interpret the varying nAChR binding of the non-native forms.A model for the binding of native GI(2-7;3-13) to the mammalian nAChR is proposed, with an alpha-subunit binding face made up of Cys2, Asn4, Pro5, Ala6 and Cys7 and a selectivity face, comprised of Arg9 and His10. These two faces orient the molecule between the alpha and delta subunits of the receptor. The structure of the CCNPAC sequence of the native GI(2-7;3-13) is compared to the structure of the identical sequence from the toxic domain of heat-stable enterotoxins, which forms part of the receptor binding region of the enterotoxins, but which has a different disulfide connectivity.

-	~~{{STRUCTURE_1xgc\| PDB=1xgc \| SCENE= }}~~	+	Structure determination of the three disulfide bond isomers of alpha-conotoxin GI: a model for the role of disulfide bonds in structural stability.,Gehrmann J, Alewood PF, Craik DJ J Mol Biol. 1998 May 1;278(2):401-15. PMID:9571060<ref>PMID:9571060</ref>

-	~~===ALPHA CONOTOXIN GI: 2-3~~;~~7-13 DISULFIDE BOND ISOMER~~, ~~NMR, 25 STRUCTURES===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_9571060}}~~	+	== References ==
-		+	<references/>
-	~~==About this Structure==~~	+	__TOC__
-	~~[[1xgc]] is~~ a ~~1 chain structure~~. ~~Full experimental information is available from [http://oca~~.~~weizmann~~.~~ac.il~~/~~oca-bin/ocashort?id=1XGC OCA].~~	+	</StructureSection>
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:009571060<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Alewood, P F.]]		[[Category: Alewood, P F.]]
	[[Category: Craik, D J.]]		[[Category: Craik, D J.]]

OCA: Protected "1xgc" [edit=sysop:move=sysop]

OCA — Tue, 14 Aug 2012 16:42:12 GMT

Protected "1xgc" [edit=sysop:move=sysop]

←Older revision

Revision as of 16:42, 14 August 2012

OCA at 16:42, 14 August 2012

OCA — Tue, 14 Aug 2012 16:42:11 GMT

←Older revision		Revision as of 16:42, 14 August 2012
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-	{{Seed}}
	[[Image:1xgc.png\|left\|200px]]		[[Image:1xgc.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_1xgc", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_1xgc\| PDB=1xgc \| SCENE= }}		{{STRUCTURE_1xgc\| PDB=1xgc \| SCENE= }}

	===ALPHA CONOTOXIN GI: 2-3;7-13 DISULFIDE BOND ISOMER, NMR, 25 STRUCTURES===		===ALPHA CONOTOXIN GI: 2-3;7-13 DISULFIDE BOND ISOMER, NMR, 25 STRUCTURES===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_9571060}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 9571060 is the PubMed ID number.
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	{{ABSTRACT_PUBMED_9571060}}		{{ABSTRACT_PUBMED_9571060}}

	==About this Structure==		==About this Structure==
-	~~1XGC is a~~ [[~~Single protein~~]] structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XGC OCA].	+	[[1xgc]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XGC OCA].

	==Reference==		==Reference==
-	Structure determination of the three disulfide bond isomers of alpha-conotoxin GI: a model for the role of disulfide bonds in structural stability., Gehrmann J, Alewood PF, Craik DJ, J Mol Biol. 1998 May 1;278(2):401-15. PMID:~~[http:~~//~~www.ncbi.nlm.nih.gov/pubmed/9571060 9571060]~~	+	<ref group="xtra">PMID:009571060</ref><references group="xtra"/>
-	~~[[Category: Single protein]]~~	+
	[[Category: Alewood, P F.]]		[[Category: Alewood, P F.]]
	[[Category: Craik, D J.]]		[[Category: Craik, D J.]]
Line 31:		Line 18:
	[[Category: Neurotoxin]]		[[Category: Neurotoxin]]
	[[Category: Nicotinic acetylcholine recepto disulfide bond isomer]]		[[Category: Nicotinic acetylcholine recepto disulfide bond isomer]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 04:23:01 2008''

OCA at 01:23, 29 July 2008

OCA — Tue, 29 Jul 2008 01:23:06 GMT

←Older revision		Revision as of 01:23, 29 July 2008
Line 1:		Line 1:
-	[[Image:1xgc.~~jpg~~\|left\|200px]]	+	{{Seed}}
		+	[[Image:1xgc.png\|left\|200px]]

	<!--		<!--
Line 9:		Line 10:
	{{STRUCTURE_1xgc\| PDB=1xgc \| SCENE= }}		{{STRUCTURE_1xgc\| PDB=1xgc \| SCENE= }}

-	~~'''~~ALPHA CONOTOXIN GI: 2-3;7-13 DISULFIDE BOND ISOMER, NMR, 25 STRUCTURES~~'''~~	+	===ALPHA CONOTOXIN GI: 2-3;7-13 DISULFIDE BOND ISOMER, NMR, 25 STRUCTURES===


-	~~==Overview==~~	+	<!--
-	~~The three possible disulfide bonded isomers of alpha~~-~~conotoxin GI have been selectively synthesised and their structures determined by 1H NMR spectroscopy. alpha~~-Conotoxin GI derives from the venom of Conus geographus and is a useful neuropharmacological tool as it selectively binds to the nicotinic acetylcholine receptor (nAChR), a ligand-gated ion channel involved in nerve signal transmission. The ~~peptide has the sequence ECCNPACGRHYSC-NH2~~, ~~and the three disulfide bonded isomers are referred to as GI(2-7;3-13)~~, ~~GI(2-13;3-7) and GI(2-3;7-13). The NMR structure for~~ the ~~native isomer GI(2-7;3-13) is of excellent quality, with a backbone pairwise RMSD of 0.16 A for a family of 35 structures, and comprises primarily a distorted 310 helix between residues 5~~ to ~~11. The two non-native isomers exhibit multiple conformers in solution, with~~ the ~~major populated forms being different in structure both from each other and from the native form. Structure-activity relationships for the native GI~~(~~2-7;3-13)~~ as ~~well as the role of the disulfide bonds~~ on ~~folding and stability of the three isomers are examined~~. ~~It is concluded that the disulfide bonds in alpha-conotoxin GI play a crucial part in determining both the structure and stability of the peptide~~. ~~A trend for increased conformational heterogeneity was observed in the order of GI(2-7;3-13~~)~~<GI(2-13;3-7)<GI(2-3;7-13). It was found that the peptide bond joining Cys2 to Cys3 in GI(2-3;7-13) is predominantly trans~~, rather than cis as theoretically predicted. These structural data are used to interpret the varying nAChR binding of the non-native forms.A model for the binding of native GI(2-7;3-13) to the mammalian nAChR is ~~proposed, with an alpha-subunit binding face made up of Cys2, Asn4, Pro5, Ala6 and Cys7 and a selectivity face, comprised of Arg9 and His10. These two faces orient~~ the ~~molecule between the alpha and delta subunits of the receptor~~. ~~The structure of the CCNPAC sequence of the native GI(2~~-~~7;3~~-13) is compared to the structure of the identical sequence from the toxic domain of heat-stable enterotoxins, which forms part of the receptor binding region of the enterotoxins, but which has a different disulfide connectivity.	+	The line below this paragraph, {{ABSTRACT_PUBMED_9571060}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 9571060 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_9571060}}

	==About this Structure==		==About this Structure==
-	1XGC is a [[Single protein]] structure. Full ~~crystallographic~~ information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XGC OCA].	+	1XGC is a [[Single protein]] structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XGC OCA].

	==Reference==		==Reference==
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	[[Category: Neurotoxin]]		[[Category: Neurotoxin]]
	[[Category: Nicotinic acetylcholine recepto disulfide bond isomer]]		[[Category: Nicotinic acetylcholine recepto disulfide bond isomer]]
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