1xjv - Revision history

OCA at 08:50, 14 February 2024

OCA — Wed, 14 Feb 2024 08:50:51 GMT

←Older revision		Revision as of 08:50, 14 February 2024
Line 3:		Line 3:
	<StructureSection load='1xjv' size='340' side='right'caption='[[1xjv]], [[Resolution\|resolution]] 1.73Å' scene=''>		<StructureSection load='1xjv' size='340' side='right'caption='[[1xjv]], [[Resolution\|resolution]] 1.73Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1xjv]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Human Human~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XJV OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=1XJV FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1xjv]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XJV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1XJV FirstGlance]. <br>
-	</td></tr><tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat">~~POT1 (~~[~~http://www~~.~~ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info~~&~~srchmode=5&id=9606 HUMAN])~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.73Å</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=1xjv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1xjv OCA], [~~http~~://pdbe.org/1xjv PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1xjv RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1xjv PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1xjv ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1xjv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1xjv OCA], [https://pdbe.org/1xjv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1xjv RCSB], [https://www.ebi.ac.uk/pdbsum/1xjv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1xjv ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/POTE1_HUMAN POTE1_HUMAN]] Component of the telomerase ribonucleoprotein (RNP) complex that is essential for the replication of chromosome termini. Is a component of the double-stranded telomeric DNA-binding TRF1 complex which is involved in the regulation of telomere length by cis-inhibition of telomerase. Also acts as a single-stranded telomeric DNA-binding protein and thus may act as a downstream effector of the TRF1 complex and may transduce information about telomere maintenance and/or length to the telomere terminus. Component of the shelterin complex (telosome) that is involved in the regulation of telomere length and protection. Shelterin associates with arrays of double-stranded TTAGGG repeats added by telomerase and protects chromosome ends; without its protective activity, telomeres are no longer hidden from the DNA damage surveillance and chromosome ends are inappropriately processed by DNA repair pathways. Binds to two or more telomeric single-stranded 5'-TTAGGG-3' repeats (G-strand) and with high specificity to a minimal telomeric single-stranded 5'-TAGGGTTAG-3' sequence. Binds telomeric single-stranded sequences internally or at proximity of a 3'-end. Its activity is TERT dependent but it does not increase TERT activity by itself. In contrast, the ACD-POT1 heterodimer enhances telomere elongation by increasing telomerase processivity.<ref>PMID:12781132</ref> <ref>PMID:12768206</ref> <ref>PMID:16166375</ref> <ref>PMID:17237768</ref> <ref>PMID:20231318</ref>	+	[https://www.uniprot.org/uniprot/POTE1_HUMAN POTE1_HUMAN] Component of the telomerase ribonucleoprotein (RNP) complex that is essential for the replication of chromosome termini. Is a component of the double-stranded telomeric DNA-binding TRF1 complex which is involved in the regulation of telomere length by cis-inhibition of telomerase. Also acts as a single-stranded telomeric DNA-binding protein and thus may act as a downstream effector of the TRF1 complex and may transduce information about telomere maintenance and/or length to the telomere terminus. Component of the shelterin complex (telosome) that is involved in the regulation of telomere length and protection. Shelterin associates with arrays of double-stranded TTAGGG repeats added by telomerase and protects chromosome ends; without its protective activity, telomeres are no longer hidden from the DNA damage surveillance and chromosome ends are inappropriately processed by DNA repair pathways. Binds to two or more telomeric single-stranded 5'-TTAGGG-3' repeats (G-strand) and with high specificity to a minimal telomeric single-stranded 5'-TAGGGTTAG-3' sequence. Binds telomeric single-stranded sequences internally or at proximity of a 3'-end. Its activity is TERT dependent but it does not increase TERT activity by itself. In contrast, the ACD-POT1 heterodimer enhances telomere elongation by increasing telomerase processivity.<ref>PMID:12781132</ref> <ref>PMID:12768206</ref> <ref>PMID:16166375</ref> <ref>PMID:17237768</ref> <ref>PMID:20231318</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 19:		Line 19:
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1xjv ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1xjv ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	The POT1 (protection of telomeres 1) protein binds the single-stranded overhang at the ends of chromosomes in diverse eukaryotes. It is essential for chromosome end-protection in the fission yeast Schizosaccharomyces pombe, and it is involved in regulation of telomere length in human cells. Here, we report the crystal structure at a resolution of 1.73 A of the N-terminal half of human POT1 (hPOT1) protein bound to a telomeric single-stranded DNA (ssDNA) decamer, TTAGGGTTAG, the minimum tight-binding sequence indicated by in vitro binding assays. The structure reveals that hPOT1 contains two oligonucleotide/ oligosaccharide-binding (OB) folds; the N-terminal OB fold binds the first six nucleotides, resembling the structure of the S. pombe Pot1pN-ssDNA complex, whereas the second OB fold binds and protects the 3' end of the ssDNA. These results provide an atomic-resolution model for chromosome end-capping.
-
-	Structure of human POT1 bound to telomeric single-stranded DNA provides a model for chromosome end-protection.,Lei M, Podell ER, Cech TR Nat Struct Mol Biol. 2004 Dec;11(12):1223-9. Epub 2004 Nov 21. PMID:15558049<ref>PMID:15558049</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 1xjv" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Human~~]]	+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Cech~~, T R~~]]	+	[[Category: Cech TR]]
-	[[Category: Lei, M]]	+	[[Category: Lei M]]
-	[[Category: Podell~~, E R]]~~	+	[[Category: Podell ER]]
-	~~[[Category: Protein-dna complex]]~~	+
-	~~[[Category: Single-stranded dna]]~~	+
-	~~[[Category: Telomere]]~~	+
-	~~[[Category: Transcription-dna complex~~]]	+

OCA at 10:03, 6 January 2021

OCA — Wed, 06 Jan 2021 10:03:18 GMT

←Older revision		Revision as of 10:03, 6 January 2021
Line 1:		Line 1:

	==Crystal structure of human POT1 bound to telomeric single-stranded DNA (TTAGGGTTAG)==		==Crystal structure of human POT1 bound to telomeric single-stranded DNA (TTAGGGTTAG)==
-	<StructureSection load='1xjv' size='340' side='right' caption='[[1xjv]], [[Resolution\|resolution]] 1.73Å' scene=''>	+	<StructureSection load='1xjv' size='340' side='right'caption='[[1xjv]], [[Resolution\|resolution]] 1.73Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1xjv]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XJV OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1XJV FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1xjv]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XJV OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=1XJV FirstGlance]. <br>
	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">POT1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>		</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">POT1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1xjv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1xjv OCA], [http://pdbe.org/1xjv PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1xjv RCSB], [http://www.ebi.ac.uk/pdbsum/1xjv PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1xjv ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=1xjv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1xjv OCA], [http://pdbe.org/1xjv PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1xjv RCSB], [http://www.ebi.ac.uk/pdbsum/1xjv PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1xjv ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 33:		Line 33:
	</StructureSection>		</StructureSection>
	[[Category: Human]]		[[Category: Human]]
		+	[[Category: Large Structures]]
	[[Category: Cech, T R]]		[[Category: Cech, T R]]
	[[Category: Lei, M]]		[[Category: Lei, M]]

OCA at 07:13, 18 April 2018

OCA — Wed, 18 Apr 2018 07:13:22 GMT

←Older revision		Revision as of 07:13, 18 April 2018
Line 1:		Line 1:
		+
	==Crystal structure of human POT1 bound to telomeric single-stranded DNA (TTAGGGTTAG)==		==Crystal structure of human POT1 bound to telomeric single-stranded DNA (TTAGGGTTAG)==
	<StructureSection load='1xjv' size='340' side='right' caption='[[1xjv]], [[Resolution\|resolution]] 1.73Å' scene=''>		<StructureSection load='1xjv' size='340' side='right' caption='[[1xjv]], [[Resolution\|resolution]] 1.73Å' scene=''>
Line 4:		Line 5:
	<table><tr><td colspan='2'>[[1xjv]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XJV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1XJV FirstGlance]. <br>		<table><tr><td colspan='2'>[[1xjv]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XJV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1XJV FirstGlance]. <br>
	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">POT1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>		</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">POT1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1xjv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1xjv OCA], [http://pdbe.org/1xjv PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1xjv RCSB], [http://www.ebi.ac.uk/pdbsum/1xjv PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1xjv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1xjv OCA], [http://pdbe.org/1xjv PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1xjv RCSB], [http://www.ebi.ac.uk/pdbsum/1xjv PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1xjv ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 12:		Line 13:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/xj/1xjv_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/xj/1xjv_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1xjv ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 23:52, 11 September 2015

OCA — Fri, 11 Sep 2015 23:52:29 GMT

←Older revision		Revision as of 23:52, 11 September 2015
Line 2:		Line 2:
	<StructureSection load='1xjv' size='340' side='right' caption='[[1xjv]], [[Resolution\|resolution]] 1.73Å' scene=''>		<StructureSection load='1xjv' size='340' side='right' caption='[[1xjv]], [[Resolution\|resolution]] 1.73Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1xjv]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Homo_sapiens Homo sapiens~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XJV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1XJV FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1xjv]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XJV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1XJV FirstGlance]. <br>
-	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">POT1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 ~~Homo sapiens~~])</td></tr>	+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">POT1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1xjv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1xjv OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1xjv RCSB], [http://www.ebi.ac.uk/pdbsum/1xjv PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1xjv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1xjv OCA], [http://pdbe.org/1xjv PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1xjv RCSB], [http://www.ebi.ac.uk/pdbsum/1xjv PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 26:		Line 26:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1xjv" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Homo sapiens~~]]	+	[[Category: Human]]
	[[Category: Cech, T R]]		[[Category: Cech, T R]]
	[[Category: Lei, M]]		[[Category: Lei, M]]

OCA at 21:03, 25 December 2014

OCA — Thu, 25 Dec 2014 21:03:53 GMT

←Older revision		Revision as of 21:03, 25 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1xjv]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XJV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1XJV FirstGlance]. <br>		<table><tr><td colspan='2'>[[1xjv]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XJV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1XJV FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">POT1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])</td></tr>	+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">POT1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1xjv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1xjv OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1xjv RCSB], [http://www.ebi.ac.uk/pdbsum/1xjv PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1xjv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1xjv OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1xjv RCSB], [http://www.ebi.ac.uk/pdbsum/1xjv PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/POTE1_HUMAN POTE1_HUMAN]] Component of the telomerase ribonucleoprotein (RNP) complex that is essential for the replication of chromosome termini. Is a component of the double-stranded telomeric DNA-binding TRF1 complex which is involved in the regulation of telomere length by cis-inhibition of telomerase. Also acts as a single-stranded telomeric DNA-binding protein and thus may act as a downstream effector of the TRF1 complex and may transduce information about telomere maintenance and/or length to the telomere terminus. Component of the shelterin complex (telosome) that is involved in the regulation of telomere length and protection. Shelterin associates with arrays of double-stranded TTAGGG repeats added by telomerase and protects chromosome ends; without its protective activity, telomeres are no longer hidden from the DNA damage surveillance and chromosome ends are inappropriately processed by DNA repair pathways. Binds to two or more telomeric single-stranded 5'-TTAGGG-3' repeats (G-strand) and with high specificity to a minimal telomeric single-stranded 5'-TAGGGTTAG-3' sequence. Binds telomeric single-stranded sequences internally or at proximity of a 3'-end. Its activity is TERT dependent but it does not increase TERT activity by itself. In contrast, the ACD-POT1 heterodimer enhances telomere elongation by increasing telomerase processivity.<ref>PMID:12781132</ref> <ref>PMID:12768206</ref> <ref>PMID:16166375</ref> <ref>PMID:17237768</ref> <ref>PMID:20231318</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 29:		Line 31:
	</StructureSection>		</StructureSection>
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
-	[[Category: Cech, T R.]]	+	[[Category: Cech, T R]]
-	[[Category: Lei, M.]]	+	[[Category: Lei, M]]
-	[[Category: Podell, E R.]]	+	[[Category: Podell, E R]]
	[[Category: Protein-dna complex]]		[[Category: Protein-dna complex]]
	[[Category: Single-stranded dna]]		[[Category: Single-stranded dna]]
	[[Category: Telomere]]		[[Category: Telomere]]
	[[Category: Transcription-dna complex]]		[[Category: Transcription-dna complex]]

OCA at 20:28, 29 September 2014

OCA — Mon, 29 Sep 2014 20:28:52 GMT

←Older revision		Revision as of 20:28, 29 September 2014
Line 1:		Line 1:
-	[[~~Image~~:1xjv.~~png\|left~~\|200px]]	+	==Crystal structure of human POT1 bound to telomeric single-stranded DNA (TTAGGGTTAG)==
		+	<StructureSection load='1xjv' size='340' side='right' caption='[[1xjv]], [[Resolution\|resolution]] 1.73Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1xjv]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XJV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1XJV FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">POT1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1xjv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1xjv OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1xjv RCSB], [http://www.ebi.ac.uk/pdbsum/1xjv PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/xj/1xjv_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The POT1 (protection of telomeres 1) protein binds the single-stranded overhang at the ends of chromosomes in diverse eukaryotes. It is essential for chromosome end-protection in the fission yeast Schizosaccharomyces pombe, and it is involved in regulation of telomere length in human cells. Here, we report the crystal structure at a resolution of 1.73 A of the N-terminal half of human POT1 (hPOT1) protein bound to a telomeric single-stranded DNA (ssDNA) decamer, TTAGGGTTAG, the minimum tight-binding sequence indicated by in vitro binding assays. The structure reveals that hPOT1 contains two oligonucleotide/ oligosaccharide-binding (OB) folds; the N-terminal OB fold binds the first six nucleotides, resembling the structure of the S. pombe Pot1pN-ssDNA complex, whereas the second OB fold binds and protects the 3' end of the ssDNA. These results provide an atomic-resolution model for chromosome end-capping.

-	~~{{STRUCTURE_1xjv\| PDB=1xjv \| SCENE= }}~~	+	Structure of human POT1 bound to telomeric single-stranded DNA provides a model for chromosome end-protection.,Lei M, Podell ER, Cech TR Nat Struct Mol Biol. 2004 Dec;11(12):1223-9. Epub 2004 Nov 21. PMID:15558049<ref>PMID:15558049</ref>

-	~~===Crystal structure~~ of ~~human POT1 bound to telomeric single-stranded DNA (TTAGGGTTAG)===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_15558049}}~~	+	== References ==
-		+	<references/>
-	~~==About this Structure==~~	+	__TOC__
-	~~[[1xjv]] is a 2 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Homo_sapiens Homo sapiens]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=1XJV OCA].~~	+	</StructureSection>
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:015558049<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
	[[Category: Cech, T R.]]		[[Category: Cech, T R.]]

OCA: Protected "1xjv" [edit=sysop:move=sysop]

OCA — Sat, 05 Jan 2013 11:11:29 GMT

Protected "1xjv" [edit=sysop:move=sysop]

←Older revision

Revision as of 11:11, 5 January 2013

OCA at 11:11, 5 January 2013

OCA — Sat, 05 Jan 2013 11:11:27 GMT

←Older revision		Revision as of 11:11, 5 January 2013
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-	{{Seed}}
	[[Image:1xjv.png\|left\|200px]]		[[Image:1xjv.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_1xjv", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_1xjv\| PDB=1xjv \| SCENE= }}		{{STRUCTURE_1xjv\| PDB=1xjv \| SCENE= }}

	===Crystal structure of human POT1 bound to telomeric single-stranded DNA (TTAGGGTTAG)===		===Crystal structure of human POT1 bound to telomeric single-stranded DNA (TTAGGGTTAG)===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_15558049}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 15558049 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_15558049}}		{{ABSTRACT_PUBMED_15558049}}

	==About this Structure==		==About this Structure==
-	~~1XJV~~ is a 2 ~~chains~~ structure ~~of sequences~~ from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XJV OCA].	+	[[1xjv]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XJV OCA].

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~15558049~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:015558049</ref><references group="xtra"/>
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
	[[Category: Cech, T R.]]		[[Category: Cech, T R.]]
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	[[Category: Single-stranded dna]]		[[Category: Single-stranded dna]]
	[[Category: Telomere]]		[[Category: Telomere]]
-		+	[[Category: Transcription-dna complex]]
-	~~''Page seeded by~~ [~~http~~:~~//oca.weizmann.ac.il/oca OCA~~ ] ~~on Wed Feb 18 07:16:10 2009''~~	+

OCA at 05:16, 18 February 2009

OCA — Wed, 18 Feb 2009 05:16:13 GMT

←Older revision		Revision as of 05:16, 18 February 2009
Line 20:		Line 20:

	==About this Structure==		==About this Structure==
-	1XJV is a ~~[[Single protein]]~~ structure of ~~sequence~~ from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XJV OCA].	+	1XJV is a 2 chains structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XJV OCA].

	==Reference==		==Reference==
-	Structure of human POT1 bound to telomeric single-stranded DNA provides a model for chromosome end-protection., Lei M, Podell ER, Cech TR, Nat Struct Mol Biol. 2004 Dec;11(12):1223-9. Epub 2004 Nov 21. PMID:~~[http:~~//~~www.ncbi.nlm.nih.gov/pubmed/15558049 15558049]~~	+	<ref group="xtra">PMID:15558049</ref><references group="xtra"/>
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
-	[[Category: Single protein]]
	[[Category: Cech, T R.]]		[[Category: Cech, T R.]]
	[[Category: Lei, M.]]		[[Category: Lei, M.]]
Line 33:		Line 32:
	[[Category: Telomere]]		[[Category: Telomere]]

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Tue Jul 29 13~~:58:~~12 2008~~''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Feb 18 07:16:10 2009''

OCA at 10:58, 29 July 2008

OCA — Tue, 29 Jul 2008 10:58:16 GMT

←Older revision		Revision as of 10:58, 29 July 2008
Line 1:		Line 1:
-	[[Image:1xjv.~~gif~~\|left\|200px]]	+	{{Seed}}
		+	[[Image:1xjv.png\|left\|200px]]

	<!--		<!--
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	{{STRUCTURE_1xjv\| PDB=1xjv \| SCENE= }}		{{STRUCTURE_1xjv\| PDB=1xjv \| SCENE= }}

-	~~'''~~Crystal structure of human POT1 bound to telomeric single-stranded DNA (TTAGGGTTAG)~~'''~~	+	===Crystal structure of human POT1 bound to telomeric single-stranded DNA (TTAGGGTTAG)===


-	~~==Overview==~~	+	<!--
-	The POT1 (protection of telomeres 1) protein binds the single-stranded overhang at the ends of chromosomes in diverse eukaryotes. It is essential for chromosome end-protection in the fission yeast Schizosaccharomyces pombe, ~~and it is involved in regulation of telomere length in human cells. Here~~, ~~we report~~ the ~~crystal structure at a resolution of 1.73 A of~~ the ~~N-terminal half of human POT1~~ (~~hPOT1) protein bound to a telomeric single-stranded DNA (ssDNA) decamer, TTAGGGTTAG, the minimum tight-binding sequence indicated by in vitro binding assays~~. ~~The structure reveals that hPOT1 contains two oligonucleotide/ oligosaccharide-binding (OB~~) ~~folds; the N-terminal OB fold binds the first six nucleotides~~, ~~resembling~~ the ~~structure of the S~~. ~~pombe Pot1pN~~-~~ssDNA complex, whereas the second OB fold binds and protects the 3' end of the ssDNA. These results provide an atomic~~-~~resolution model for chromosome end-capping.~~	+	The line below this paragraph, {{ABSTRACT_PUBMED_15558049}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 15558049 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_15558049}}

	==About this Structure==		==About this Structure==
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	[[Category: Single-stranded dna]]		[[Category: Single-stranded dna]]
	[[Category: Telomere]]		[[Category: Telomere]]
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Sat May 3 15~~:07:22 2008''	+
		+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 13:58:12 2008''