1xo6 - Revision history

OCA at 08:51, 14 February 2024

OCA — Wed, 14 Feb 2024 08:51:59 GMT

←Older revision		Revision as of 08:51, 14 February 2024
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	<StructureSection load='1xo6' size='340' side='right'caption='[[1xo6]], [[Resolution\|resolution]] 2.20Å' scene=''>		<StructureSection load='1xo6' size='340' side='right'caption='[[1xo6]], [[Resolution\|resolution]] 2.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1xo6]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/~~"actinomyces_coelicolor"_(muller_1908)_lieske_1921 "actinomyces~~ coelicolor~~" (muller 1908) lieske 1921~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XO6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1XO6 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1xo6]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_coelicolor Streptomyces coelicolor]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XO6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1XO6 FirstGlance]. <br>
-	</td></tr><tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat">~~<div style='overflow: auto; max~~-~~height: 3em;'>[[1xnv\|1xnv]]~~, [[~~1xnw~~\|~~1xnw~~]]~~, [[1xny\|1xny]]</div></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.2Å</td></tr>
-	~~<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en~~.~~wikipedia.org/wiki/Propionyl-CoA_carboxylase Propionyl-CoA carboxylase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.4.1.3 6.4.1.3] </span>~~</td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1xo6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1xo6 OCA], [https://pdbe.org/1xo6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1xo6 RCSB], [https://www.ebi.ac.uk/pdbsum/1xo6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1xo6 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1xo6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1xo6 OCA], [https://pdbe.org/1xo6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1xo6 RCSB], [https://www.ebi.ac.uk/pdbsum/1xo6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1xo6 ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/Q9X4K7_STRCH Q9X4K7_STRCH]
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1xo6 ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1xo6 ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	Acetyl-CoA carboxylase (ACC) and propionyl-CoA carboxylase (PCC) catalyze the carboxylation of acetyl- and propionyl-CoA to generate malonyl- and methylmalonyl-CoA, respectively. Understanding the substrate specificity of ACC and PCC will (1) help in the development of novel structure-based inhibitors that are potential therapeutics against obesity, cancer, and infectious disease and (2) facilitate bioengineering to provide novel extender units for polyketide biosynthesis. ACC and PCC in Streptomyces coelicolor are multisubunit complexes. The core catalytic beta-subunits, PccB and AccB, are 360 kDa homohexamers, catalyzing the transcarboxylation between biotin and acyl-CoAs. Apo and substrate-bound crystal structures of PccB hexamers were determined to 2.0-2.8 A. The hexamer assembly forms a ring-shaped complex. The hydrophobic, highly conserved biotin-binding pocket was identified for the first time. Biotin and propionyl-CoA bind perpendicular to each other in the active site, where two oxyanion holes were identified. N1 of biotin is proposed to be the active site base. Structure-based mutagenesis at a single residue of PccB and AccB allowed interconversion of the substrate specificity of ACC and PCC. The di-domain, dimeric interaction is crucial for enzyme catalysis, stability, and substrate specificity; these features are also highly conserved among biotin-dependent carboxyltransferases. Our findings enable bioengineering of the acyl-CoA carboxylase (ACCase) substrate specificity to provide novel extender units for the combinatorial biosynthesis of polyketides.
-
-	Crystal structure of the beta-subunit of acyl-CoA carboxylase: structure-based engineering of substrate specificity.,Diacovich L, Mitchell DL, Pham H, Gago G, Melgar MM, Khosla C, Gramajo H, Tsai SC Biochemistry. 2004 Nov 9;43(44):14027-36. PMID:15518551<ref>PMID:15518551</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 1xo6" style="background-color:#fffaf0;"></div>
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Propionyl-CoA carboxylase~~]]	+	[[Category: Streptomyces coelicolor]]
-	[[Category: Diacovich, L]]	+	[[Category: Diacovich L]]
-	[[Category: Gago, G]]	+	[[Category: Gago G]]
-	[[Category: Gramajo, H]]	+	[[Category: Gramajo H]]
-	[[Category: Khosla, C]]	+	[[Category: Khosla C]]
-	[[Category: Melgar~~, M M~~]]	+	[[Category: Melgar MM]]
-	[[Category: Mitchell~~, D L~~]]	+	[[Category: Mitchell DL]]
-	[[Category: Pham, H]]	+	[[Category: Pham H]]
-	[[Category: Tsai, S C]]	+	[[Category: Tsai S-C]]
-	~~[[Category: Acyl-coa carboxylase]]~~	+
-	~~[[Category: Carboxyltransferase]]~~	+
-	~~[[Category: Ligase]]~~	+
-	~~[[Category: Polyketide]]~~	+
-	~~[[Category: Polyketide synthase~~]]	+

OCA at 16:40, 27 October 2021

OCA — Wed, 27 Oct 2021 16:40:29 GMT

←Older revision		Revision as of 16:40, 27 October 2021
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	<StructureSection load='1xo6' size='340' side='right'caption='[[1xo6]], [[Resolution\|resolution]] 2.20Å' scene=''>		<StructureSection load='1xo6' size='340' side='right'caption='[[1xo6]], [[Resolution\|resolution]] 2.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1xo6]] is a 6 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/"actinomyces_coelicolor"_(muller_1908)_lieske_1921 "actinomyces coelicolor" (muller 1908) lieske 1921]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XO6 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1XO6 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1xo6]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/"actinomyces_coelicolor"_(muller_1908)_lieske_1921 "actinomyces coelicolor" (muller 1908) lieske 1921]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XO6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1XO6 FirstGlance]. <br>
-	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1xnv\|1xnv]], [[1xnw\|1xnw]], [[1xny\|1xny]]</td></tr>	+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1xnv\|1xnv]], [[1xnw\|1xnw]], [[1xny\|1xny]]</div></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Propionyl-CoA_carboxylase Propionyl-CoA carboxylase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.4.1.3 6.4.1.3] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Propionyl-CoA_carboxylase Propionyl-CoA carboxylase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.4.1.3 6.4.1.3] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1xo6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1xo6 OCA], [~~http~~://pdbe.org/1xo6 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1xo6 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1xo6 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1xo6 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1xo6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1xo6 OCA], [https://pdbe.org/1xo6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1xo6 RCSB], [https://www.ebi.ac.uk/pdbsum/1xo6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1xo6 ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==

OCA at 17:18, 22 January 2020

OCA — Wed, 22 Jan 2020 17:18:13 GMT

←Older revision		Revision as of 17:18, 22 January 2020
Line 1:		Line 1:

	==Acyl-CoA Carboxylase Beta Subunit from S. coelicolor (PccB), apo form #3==		==Acyl-CoA Carboxylase Beta Subunit from S. coelicolor (PccB), apo form #3==
-	<StructureSection load='1xo6' size='340' side='right' caption='[[1xo6]], [[Resolution\|resolution]] 2.20Å' scene=''>	+	<StructureSection load='1xo6' size='340' side='right'caption='[[1xo6]], [[Resolution\|resolution]] 2.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1xo6]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/"actinomyces_coelicolor"_(muller_1908)_lieske_1921 "actinomyces coelicolor" (muller 1908) lieske 1921]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XO6 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1XO6 FirstGlance]. <br>		<table><tr><td colspan='2'>[[1xo6]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/"actinomyces_coelicolor"_(muller_1908)_lieske_1921 "actinomyces coelicolor" (muller 1908) lieske 1921]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XO6 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1XO6 FirstGlance]. <br>
Line 31:		Line 31:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Propionyl-CoA carboxylase]]		[[Category: Propionyl-CoA carboxylase]]
	[[Category: Diacovich, L]]		[[Category: Diacovich, L]]

OCA at 07:02, 18 April 2018

OCA — Wed, 18 Apr 2018 07:02:49 GMT

←Older revision		Revision as of 07:02, 18 April 2018
Line 1:		Line 1:
		+
	==Acyl-CoA Carboxylase Beta Subunit from S. coelicolor (PccB), apo form #3==		==Acyl-CoA Carboxylase Beta Subunit from S. coelicolor (PccB), apo form #3==
	<StructureSection load='1xo6' size='340' side='right' caption='[[1xo6]], [[Resolution\|resolution]] 2.20Å' scene=''>		<StructureSection load='1xo6' size='340' side='right' caption='[[1xo6]], [[Resolution\|resolution]] 2.20Å' scene=''>
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	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1xnv\|1xnv]], [[1xnw\|1xnw]], [[1xny\|1xny]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1xnv\|1xnv]], [[1xnw\|1xnw]], [[1xny\|1xny]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Propionyl-CoA_carboxylase Propionyl-CoA carboxylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.4.1.3 6.4.1.3] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Propionyl-CoA_carboxylase Propionyl-CoA carboxylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.4.1.3 6.4.1.3] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1xo6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1xo6 OCA], [http://pdbe.org/1xo6 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1xo6 RCSB], [http://www.ebi.ac.uk/pdbsum/1xo6 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1xo6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1xo6 OCA], [http://pdbe.org/1xo6 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1xo6 RCSB], [http://www.ebi.ac.uk/pdbsum/1xo6 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1xo6 ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
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	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/xo/1xo6_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/xo/1xo6_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>

OCA at 16:21, 7 February 2016

OCA — Sun, 07 Feb 2016 16:21:01 GMT

←Older revision		Revision as of 16:21, 7 February 2016
Line 15:		Line 15:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1xo6 ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 03:11, 10 September 2015

OCA — Thu, 10 Sep 2015 03:11:19 GMT

←Older revision		Revision as of 03:11, 10 September 2015
Line 2:		Line 2:
	<StructureSection load='1xo6' size='340' side='right' caption='[[1xo6]], [[Resolution\|resolution]] 2.20Å' scene=''>		<StructureSection load='1xo6' size='340' side='right' caption='[[1xo6]], [[Resolution\|resolution]] 2.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1xo6]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Streptomyces_coelicolor Streptomyces~~ coelicolor]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XO6 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1XO6 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1xo6]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/"actinomyces_coelicolor"_(muller_1908)_lieske_1921 "actinomyces coelicolor" (muller 1908) lieske 1921]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XO6 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1XO6 FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1xnv\|1xnv]], [[1xnw\|1xnw]], [[1xny\|1xny]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1xnv\|1xnv]], [[1xnw\|1xnw]], [[1xny\|1xny]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Propionyl-CoA_carboxylase Propionyl-CoA carboxylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.4.1.3 6.4.1.3] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Propionyl-CoA_carboxylase Propionyl-CoA carboxylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.4.1.3 6.4.1.3] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1xo6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1xo6 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1xo6 RCSB], [http://www.ebi.ac.uk/pdbsum/1xo6 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1xo6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1xo6 OCA], [http://pdbe.org/1xo6 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1xo6 RCSB], [http://www.ebi.ac.uk/pdbsum/1xo6 PDBsum]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
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	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1xo6" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
Line 30:		Line 31:
	</StructureSection>		</StructureSection>
	[[Category: Propionyl-CoA carboxylase]]		[[Category: Propionyl-CoA carboxylase]]
-	[[Category: Streptomyces coelicolor]]
	[[Category: Diacovich, L]]		[[Category: Diacovich, L]]
	[[Category: Gago, G]]		[[Category: Gago, G]]

OCA at 13:25, 6 January 2015

OCA — Tue, 06 Jan 2015 13:25:23 GMT

←Older revision		Revision as of 13:25, 6 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1xo6]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptomyces_coelicolor Streptomyces coelicolor]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XO6 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1XO6 FirstGlance]. <br>		<table><tr><td colspan='2'>[[1xo6]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptomyces_coelicolor Streptomyces coelicolor]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XO6 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1XO6 FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1xnv\|1xnv]], [[1xnw\|1xnw]], [[1xny\|1xny]]</td></tr>	+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1xnv\|1xnv]], [[1xnw\|1xnw]], [[1xny\|1xny]]</td></tr>
-	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Propionyl-CoA_carboxylase Propionyl-CoA carboxylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.4.1.3 6.4.1.3] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Propionyl-CoA_carboxylase Propionyl-CoA carboxylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.4.1.3 6.4.1.3] </span></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1xo6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1xo6 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1xo6 RCSB], [http://www.ebi.ac.uk/pdbsum/1xo6 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1xo6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1xo6 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1xo6 RCSB], [http://www.ebi.ac.uk/pdbsum/1xo6 PDBsum]</span></td></tr>
-	<table>	+	</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 31:		Line 31:
	[[Category: Propionyl-CoA carboxylase]]		[[Category: Propionyl-CoA carboxylase]]
	[[Category: Streptomyces coelicolor]]		[[Category: Streptomyces coelicolor]]
-	[[Category: Diacovich, L.]]	+	[[Category: Diacovich, L]]
-	[[Category: Gago, G.]]	+	[[Category: Gago, G]]
-	[[Category: Gramajo, H.]]	+	[[Category: Gramajo, H]]
-	[[Category: Khosla, C.]]	+	[[Category: Khosla, C]]
-	[[Category: Melgar, M M.]]	+	[[Category: Melgar, M M]]
-	[[Category: Mitchell, D L.]]	+	[[Category: Mitchell, D L]]
-	[[Category: Pham, H.]]	+	[[Category: Pham, H]]
-	[[Category: Tsai, S C.]]	+	[[Category: Tsai, S C]]
	[[Category: Acyl-coa carboxylase]]		[[Category: Acyl-coa carboxylase]]
	[[Category: Carboxyltransferase]]		[[Category: Carboxyltransferase]]

OCA at 21:46, 29 September 2014

OCA — Mon, 29 Sep 2014 21:46:32 GMT

←Older revision		Revision as of 21:46, 29 September 2014
Line 1:		Line 1:
-	[[~~Image:~~1xo6.~~png~~\|~~left~~\|200px]]	+	==Acyl-CoA Carboxylase Beta Subunit from S. coelicolor (PccB), apo form #3==
		+	<StructureSection load='1xo6' size='340' side='right' caption='[[1xo6]], [[Resolution\|resolution]] 2.20Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1xo6]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptomyces_coelicolor Streptomyces coelicolor]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XO6 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1XO6 FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1xnv\|1xnv]], [[1xnw\|1xnw]], [[1xny\|1xny]]</td></tr>
		+	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Propionyl-CoA_carboxylase Propionyl-CoA carboxylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.4.1.3 6.4.1.3] </span></td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1xo6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1xo6 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1xo6 RCSB], [http://www.ebi.ac.uk/pdbsum/1xo6 PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/xo/1xo6_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Acetyl-CoA carboxylase (ACC) and propionyl-CoA carboxylase (PCC) catalyze the carboxylation of acetyl- and propionyl-CoA to generate malonyl- and methylmalonyl-CoA, respectively. Understanding the substrate specificity of ACC and PCC will (1) help in the development of novel structure-based inhibitors that are potential therapeutics against obesity, cancer, and infectious disease and (2) facilitate bioengineering to provide novel extender units for polyketide biosynthesis. ACC and PCC in Streptomyces coelicolor are multisubunit complexes. The core catalytic beta-subunits, PccB and AccB, are 360 kDa homohexamers, catalyzing the transcarboxylation between biotin and acyl-CoAs. Apo and substrate-bound crystal structures of PccB hexamers were determined to 2.0-2.8 A. The hexamer assembly forms a ring-shaped complex. The hydrophobic, highly conserved biotin-binding pocket was identified for the first time. Biotin and propionyl-CoA bind perpendicular to each other in the active site, where two oxyanion holes were identified. N1 of biotin is proposed to be the active site base. Structure-based mutagenesis at a single residue of PccB and AccB allowed interconversion of the substrate specificity of ACC and PCC. The di-domain, dimeric interaction is crucial for enzyme catalysis, stability, and substrate specificity; these features are also highly conserved among biotin-dependent carboxyltransferases. Our findings enable bioengineering of the acyl-CoA carboxylase (ACCase) substrate specificity to provide novel extender units for the combinatorial biosynthesis of polyketides.

-	~~{{STRUCTURE_1xo6\| PDB=1xo6 \| SCENE= }}~~	+	Crystal structure of the beta-subunit of acyl-CoA carboxylase: structure-based engineering of substrate specificity.,Diacovich L, Mitchell DL, Pham H, Gago G, Melgar MM, Khosla C, Gramajo H, Tsai SC Biochemistry. 2004 Nov 9;43(44):14027-36. PMID:15518551<ref>PMID:15518551</ref>

-	~~===Acyl-CoA Carboxylase Beta Subunit from S. coelicolor (PccB)~~, ~~apo form #3===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_15518551}}~~	+	== References ==
-		+	<references/>
-	~~==About this Structure==~~	+	__TOC__
-	~~[[1xo6]] is~~ a ~~6 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Streptomyces_coelicolor Streptomyces coelicolor]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=1XO6 OCA].~~	+	</StructureSection>
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:015518551<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Propionyl-CoA carboxylase]]		[[Category: Propionyl-CoA carboxylase]]
	[[Category: Streptomyces coelicolor]]		[[Category: Streptomyces coelicolor]]

OCA: Protected "1xo6" [edit=sysop:move=sysop]

OCA — Sat, 05 Jan 2013 13:14:51 GMT

Protected "1xo6" [edit=sysop:move=sysop]

←Older revision

Revision as of 13:14, 5 January 2013

OCA at 13:14, 5 January 2013

OCA — Sat, 05 Jan 2013 13:14:48 GMT

←Older revision		Revision as of 13:14, 5 January 2013
Line 1:		Line 1:
-	{{Seed}}
	[[Image:1xo6.png\|left\|200px]]		[[Image:1xo6.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_1xo6", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_1xo6\| PDB=1xo6 \| SCENE= }}		{{STRUCTURE_1xo6\| PDB=1xo6 \| SCENE= }}

	===Acyl-CoA Carboxylase Beta Subunit from S. coelicolor (PccB), apo form #3===		===Acyl-CoA Carboxylase Beta Subunit from S. coelicolor (PccB), apo form #3===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_15518551}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 15518551 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_15518551}}		{{ABSTRACT_PUBMED_15518551}}

	==About this Structure==		==About this Structure==
-	~~1XO6~~ is a 6 ~~chains~~ structure ~~of sequences~~ from [http://en.wikipedia.org/wiki/Streptomyces_coelicolor Streptomyces coelicolor]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XO6 OCA].	+	[[1xo6]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptomyces_coelicolor Streptomyces coelicolor]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XO6 OCA].

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~15518551~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:015518551</ref><references group="xtra"/>
	[[Category: Propionyl-CoA carboxylase]]		[[Category: Propionyl-CoA carboxylase]]
	[[Category: Streptomyces coelicolor]]		[[Category: Streptomyces coelicolor]]
Line 36:		Line 24:
	[[Category: Acyl-coa carboxylase]]		[[Category: Acyl-coa carboxylase]]
	[[Category: Carboxyltransferase]]		[[Category: Carboxyltransferase]]
		+	[[Category: Ligase]]
	[[Category: Polyketide]]		[[Category: Polyketide]]
	[[Category: Polyketide synthase]]		[[Category: Polyketide synthase]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Feb 16 15:04:16 2009''