1xse - Revision history

OCA at 13:36, 13 March 2024

OCA — Wed, 13 Mar 2024 13:36:58 GMT

←Older revision		Revision as of 13:36, 13 March 2024
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	<StructureSection load='1xse' size='340' side='right'caption='[[1xse]], [[Resolution\|resolution]] 2.50Å' scene=''>		<StructureSection load='1xse' size='340' side='right'caption='[[1xse]], [[Resolution\|resolution]] 2.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1xse]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Cavpo Cavpo~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XSE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1XSE FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1xse]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Cavia_porcellus Cavia porcellus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XSE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1XSE FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=NDP:NADPH+DIHYDRO~~-~~NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NDP</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.5Å</td></tr>
-	<tr id='~~activity~~'><td class="sblockLbl"><b>~~Activity~~:</b></td><td class="sblockDat"><~~span class~~='~~plainlinks'>[https~~:~~//en.wikipedia.org/wiki/11~~-~~beta~~-~~hydroxysteroid_dehydrogenase 11~~-~~beta-hydroxysteroid dehydrogenase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.146 1.1.1.146]~~ </~~span~~></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NDP:NADPH+DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NDP</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1xse FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1xse OCA], [https://pdbe.org/1xse PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1xse RCSB], [https://www.ebi.ac.uk/pdbsum/1xse PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1xse ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1xse FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1xse OCA], [https://pdbe.org/1xse PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1xse RCSB], [https://www.ebi.ac.uk/pdbsum/1xse PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1xse ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/DHI1_CAVPO DHI1_CAVPO]] Catalyzes reversibly the conversion of cortisol to the inactive metabolite cortisone. Catalyzes reversibly the conversion of 7-ketocholesterol to 7-beta-hydroxycholesterol. In intact cells, the reaction runs only in one direction, from 7-ketocholesterol to 7-beta-hydroxycholesterol (By similarity).<ref>PMID:10699594</ref>	+	[https://www.uniprot.org/uniprot/DHI1_CAVPO DHI1_CAVPO] Catalyzes reversibly the conversion of cortisol to the inactive metabolite cortisone. Catalyzes reversibly the conversion of 7-ketocholesterol to 7-beta-hydroxycholesterol. In intact cells, the reaction runs only in one direction, from 7-ketocholesterol to 7-beta-hydroxycholesterol (By similarity).<ref>PMID:10699594</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 20:		Line 20:
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1xse ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1xse ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	The metabolic reduction of 11-keto groups in glucocorticoid steroids such as cortisone leads to the nuclear receptor ligand cortisol. This conversion is an example of pre-receptor regulation and constitutes a novel pharmacological target for the treatment of metabolic disorders such as insulin resistance and possibly other derangements observed in the metabolic syndrome, such as hyperlipidemia, hypertension, and lowered insulin secretion. This reaction is carried out by the NADPH-dependent type 1 11beta-hydroxysteroid dehydrogenase (11beta-HSD1), an enzyme attached through an integral N-terminal transmembrane helix to the lipid bilayer and located with its active site within the lumen of the endoplasmic reticulum. Here we report the crystal structure of recombinant guinea pig 11beta-HSD1. This variant was determined in complex with NADP at 2.5 A resolution and crystallized in the presence of detergent and guanidinium hydrochloride. The overall structure of guinea pig 11beta-HSD1 shows a clear relationship to other members of the superfamily of short-chain dehydrogenases/reductases but harbors a unique C-terminal helical segment that fulfills three essential functions and accordingly is involved in subunit interactions, contributes to active site architecture, and is necessary for lipid-membrane interactions. The structure provides a model for enzyme-lipid bilayer interactions and suggests a funneling of lipophilic substrates such as steroid hormones from the hydrophobic membrane environment to the enzyme active site.
-
-	The crystal structure of guinea pig 11beta-hydroxysteroid dehydrogenase type 1 provides a model for enzyme-lipid bilayer interactions.,Ogg D, Elleby B, Norstrom C, Stefansson K, Abrahmsen L, Oppermann U, Svensson S J Biol Chem. 2005 Feb 4;280(5):3789-94. Epub 2004 Nov 12. PMID:15542590<ref>PMID:15542590</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 1xse" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 36:		Line 27:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~11-beta-hydroxysteroid dehydrogenase]]~~	+	[[Category: Cavia porcellus]]
-	~~[[Category: Cavpo~~]]	+
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Abrahmsen, L]]	+	[[Category: Abrahmsen L]]
-	[[Category: Elleby, B]]	+	[[Category: Elleby B]]
-	[[Category: Norstrom, C]]	+	[[Category: Norstrom C]]
-	[[Category: Ogg, D]]	+	[[Category: Ogg D]]
-	[[Category: Oppermann, U]]	+	[[Category: Oppermann U]]
-	[[Category: Stefansson, K]]	+	[[Category: Stefansson K]]
-	[[Category: Svensson, S]]	+	[[Category: Svensson S]]
-	~~[[Category: 11beta-hydroxysteroid dehydrogenase dimer]]~~	+
-	~~[[Category: Oxidoreductase~~]]	+

OCA at 11:27, 16 February 2022

OCA — Wed, 16 Feb 2022 11:27:30 GMT

←Older revision		Revision as of 11:27, 16 February 2022
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	<StructureSection load='1xse' size='340' side='right'caption='[[1xse]], [[Resolution\|resolution]] 2.50Å' scene=''>		<StructureSection load='1xse' size='340' side='right'caption='[[1xse]], [[Resolution\|resolution]] 2.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1xse]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Cavpo Cavpo]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XSE OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=1XSE FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1xse]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Cavpo Cavpo]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XSE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1XSE FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NDP:NADPH+DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NDP</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NDP:NADPH+DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NDP</scene></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/11-beta-hydroxysteroid_dehydrogenase 11-beta-hydroxysteroid dehydrogenase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.146 1.1.1.146] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/11-beta-hydroxysteroid_dehydrogenase 11-beta-hydroxysteroid dehydrogenase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.146 1.1.1.146] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=1xse FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1xse OCA], [~~http~~://pdbe.org/1xse PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1xse RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1xse PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1xse ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1xse FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1xse OCA], [https://pdbe.org/1xse PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1xse RCSB], [https://www.ebi.ac.uk/pdbsum/1xse PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1xse ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/DHI1_CAVPO DHI1_CAVPO]] Catalyzes reversibly the conversion of cortisol to the inactive metabolite cortisone. Catalyzes reversibly the conversion of 7-ketocholesterol to 7-beta-hydroxycholesterol. In intact cells, the reaction runs only in one direction, from 7-ketocholesterol to 7-beta-hydroxycholesterol (By similarity).<ref>PMID:10699594</ref>	+	[[https://www.uniprot.org/uniprot/DHI1_CAVPO DHI1_CAVPO]] Catalyzes reversibly the conversion of cortisol to the inactive metabolite cortisone. Catalyzes reversibly the conversion of 7-ketocholesterol to 7-beta-hydroxycholesterol. In intact cells, the reaction runs only in one direction, from 7-ketocholesterol to 7-beta-hydroxycholesterol (By similarity).<ref>PMID:10699594</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 06:13, 13 May 2020

OCA — Wed, 13 May 2020 06:13:26 GMT

←Older revision		Revision as of 06:13, 13 May 2020
Line 1:		Line 1:

	==Crystal Structure of Guinea Pig 11beta-Hydroxysteroid Dehydrogenase Type 1==		==Crystal Structure of Guinea Pig 11beta-Hydroxysteroid Dehydrogenase Type 1==
-	<StructureSection load='1xse' size='340' side='right' caption='[[1xse]], [[Resolution\|resolution]] 2.50Å' scene=''>	+	<StructureSection load='1xse' size='340' side='right'caption='[[1xse]], [[Resolution\|resolution]] 2.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1xse]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Cavpo Cavpo]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XSE OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1XSE FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1xse]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Cavpo Cavpo]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XSE OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=1XSE FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NDP:NADPH+DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NDP</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NDP:NADPH+DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NDP</scene></td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/11-beta-hydroxysteroid_dehydrogenase 11-beta-hydroxysteroid dehydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.146 1.1.1.146] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/11-beta-hydroxysteroid_dehydrogenase 11-beta-hydroxysteroid dehydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.146 1.1.1.146] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1xse FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1xse OCA], [http://pdbe.org/1xse PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1xse RCSB], [http://www.ebi.ac.uk/pdbsum/1xse PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1xse ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=1xse FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1xse OCA], [http://pdbe.org/1xse PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1xse RCSB], [http://www.ebi.ac.uk/pdbsum/1xse PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1xse ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 31:		Line 31:

	==See Also==		==See Also==
-	*[[Hydroxysteroid dehydrogenase\|Hydroxysteroid dehydrogenase]]	+	*[[Hydroxysteroid dehydrogenase 3D structures\|Hydroxysteroid dehydrogenase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 38:		Line 38:
	[[Category: 11-beta-hydroxysteroid dehydrogenase]]		[[Category: 11-beta-hydroxysteroid dehydrogenase]]
	[[Category: Cavpo]]		[[Category: Cavpo]]
		+	[[Category: Large Structures]]
	[[Category: Abrahmsen, L]]		[[Category: Abrahmsen, L]]
	[[Category: Elleby, B]]		[[Category: Elleby, B]]

OCA at 07:13, 18 April 2018

OCA — Wed, 18 Apr 2018 07:13:11 GMT

←Older revision		Revision as of 07:13, 18 April 2018
Line 1:		Line 1:
		+
	==Crystal Structure of Guinea Pig 11beta-Hydroxysteroid Dehydrogenase Type 1==		==Crystal Structure of Guinea Pig 11beta-Hydroxysteroid Dehydrogenase Type 1==
	<StructureSection load='1xse' size='340' side='right' caption='[[1xse]], [[Resolution\|resolution]] 2.50Å' scene=''>		<StructureSection load='1xse' size='340' side='right' caption='[[1xse]], [[Resolution\|resolution]] 2.50Å' scene=''>
Line 5:		Line 6:
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NDP:NADPH+DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NDP</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NDP:NADPH+DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NDP</scene></td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/11-beta-hydroxysteroid_dehydrogenase 11-beta-hydroxysteroid dehydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.146 1.1.1.146] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/11-beta-hydroxysteroid_dehydrogenase 11-beta-hydroxysteroid dehydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.146 1.1.1.146] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1xse FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1xse OCA], [http://pdbe.org/1xse PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1xse RCSB], [http://www.ebi.ac.uk/pdbsum/1xse PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1xse FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1xse OCA], [http://pdbe.org/1xse PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1xse RCSB], [http://www.ebi.ac.uk/pdbsum/1xse PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1xse ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 13:		Line 14:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/xs/1xse_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/xs/1xse_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1xse ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 23:52, 11 September 2015

OCA — Fri, 11 Sep 2015 23:52:22 GMT

←Older revision		Revision as of 23:52, 11 September 2015
Line 2:		Line 2:
	<StructureSection load='1xse' size='340' side='right' caption='[[1xse]], [[Resolution\|resolution]] 2.50Å' scene=''>		<StructureSection load='1xse' size='340' side='right' caption='[[1xse]], [[Resolution\|resolution]] 2.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1xse]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Cavia_porcellus Cavia porcellus~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XSE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1XSE FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1xse]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Cavpo Cavpo]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XSE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1XSE FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NDP:NADPH+DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NDP</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NDP:NADPH+DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NDP</scene></td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/11-beta-hydroxysteroid_dehydrogenase 11-beta-hydroxysteroid dehydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.146 1.1.1.146] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/11-beta-hydroxysteroid_dehydrogenase 11-beta-hydroxysteroid dehydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.146 1.1.1.146] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1xse FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1xse OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1xse RCSB], [http://www.ebi.ac.uk/pdbsum/1xse PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1xse FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1xse OCA], [http://pdbe.org/1xse PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1xse RCSB], [http://www.ebi.ac.uk/pdbsum/1xse PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 27:		Line 27:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1xse" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 35:		Line 36:
	</StructureSection>		</StructureSection>
	[[Category: 11-beta-hydroxysteroid dehydrogenase]]		[[Category: 11-beta-hydroxysteroid dehydrogenase]]
-	[[Category: ~~Cavia porcellus~~]]	+	[[Category: Cavpo]]
	[[Category: Abrahmsen, L]]		[[Category: Abrahmsen, L]]
	[[Category: Elleby, B]]		[[Category: Elleby, B]]

OCA at 21:03, 25 December 2014

OCA — Thu, 25 Dec 2014 21:03:45 GMT

←Older revision		Revision as of 21:03, 25 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1xse]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Cavia_porcellus Cavia porcellus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XSE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1XSE FirstGlance]. <br>		<table><tr><td colspan='2'>[[1xse]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Cavia_porcellus Cavia porcellus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XSE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1XSE FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NDP:NADPH+DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NDP</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NDP:NADPH+DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NDP</scene></td></tr>
-	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/11-beta-hydroxysteroid_dehydrogenase 11-beta-hydroxysteroid dehydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.146 1.1.1.146] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/11-beta-hydroxysteroid_dehydrogenase 11-beta-hydroxysteroid dehydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.146 1.1.1.146] </span></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1xse FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1xse OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1xse RCSB], [http://www.ebi.ac.uk/pdbsum/1xse PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1xse FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1xse OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1xse RCSB], [http://www.ebi.ac.uk/pdbsum/1xse PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/DHI1_CAVPO DHI1_CAVPO]] Catalyzes reversibly the conversion of cortisol to the inactive metabolite cortisone. Catalyzes reversibly the conversion of 7-ketocholesterol to 7-beta-hydroxycholesterol. In intact cells, the reaction runs only in one direction, from 7-ketocholesterol to 7-beta-hydroxycholesterol (By similarity).<ref>PMID:10699594</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 34:		Line 36:
	[[Category: 11-beta-hydroxysteroid dehydrogenase]]		[[Category: 11-beta-hydroxysteroid dehydrogenase]]
	[[Category: Cavia porcellus]]		[[Category: Cavia porcellus]]
-	[[Category: Abrahmsen, L.]]	+	[[Category: Abrahmsen, L]]
-	[[Category: Elleby, B.]]	+	[[Category: Elleby, B]]
-	[[Category: Norstrom, C.]]	+	[[Category: Norstrom, C]]
-	[[Category: Ogg, D.]]	+	[[Category: Ogg, D]]
-	[[Category: Oppermann, U.]]	+	[[Category: Oppermann, U]]
-	[[Category: Stefansson, K.]]	+	[[Category: Stefansson, K]]
-	[[Category: Svensson, S.]]	+	[[Category: Svensson, S]]
	[[Category: 11beta-hydroxysteroid dehydrogenase dimer]]		[[Category: 11beta-hydroxysteroid dehydrogenase dimer]]
	[[Category: Oxidoreductase]]		[[Category: Oxidoreductase]]

OCA at 22:21, 28 September 2014

OCA — Sun, 28 Sep 2014 22:21:48 GMT

←Older revision		Revision as of 22:21, 28 September 2014
Line 1:		Line 1:
-	[[~~Image~~:1xse.~~png\|left~~\|200px]]	+	==Crystal Structure of Guinea Pig 11beta-Hydroxysteroid Dehydrogenase Type 1==
		+	<StructureSection load='1xse' size='340' side='right' caption='[[1xse]], [[Resolution\|resolution]] 2.50Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1xse]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Cavia_porcellus Cavia porcellus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XSE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1XSE FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NDP:NADPH+DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NDP</scene><br>
		+	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/11-beta-hydroxysteroid_dehydrogenase 11-beta-hydroxysteroid dehydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.146 1.1.1.146] </span></td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1xse FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1xse OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1xse RCSB], [http://www.ebi.ac.uk/pdbsum/1xse PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/xs/1xse_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The metabolic reduction of 11-keto groups in glucocorticoid steroids such as cortisone leads to the nuclear receptor ligand cortisol. This conversion is an example of pre-receptor regulation and constitutes a novel pharmacological target for the treatment of metabolic disorders such as insulin resistance and possibly other derangements observed in the metabolic syndrome, such as hyperlipidemia, hypertension, and lowered insulin secretion. This reaction is carried out by the NADPH-dependent type 1 11beta-hydroxysteroid dehydrogenase (11beta-HSD1), an enzyme attached through an integral N-terminal transmembrane helix to the lipid bilayer and located with its active site within the lumen of the endoplasmic reticulum. Here we report the crystal structure of recombinant guinea pig 11beta-HSD1. This variant was determined in complex with NADP at 2.5 A resolution and crystallized in the presence of detergent and guanidinium hydrochloride. The overall structure of guinea pig 11beta-HSD1 shows a clear relationship to other members of the superfamily of short-chain dehydrogenases/reductases but harbors a unique C-terminal helical segment that fulfills three essential functions and accordingly is involved in subunit interactions, contributes to active site architecture, and is necessary for lipid-membrane interactions. The structure provides a model for enzyme-lipid bilayer interactions and suggests a funneling of lipophilic substrates such as steroid hormones from the hydrophobic membrane environment to the enzyme active site.

-	~~{{STRUCTURE_1xse\| PDB=1xse \| SCENE= }}~~	+	The crystal structure of guinea pig 11beta-hydroxysteroid dehydrogenase type 1 provides a model for enzyme-lipid bilayer interactions.,Ogg D, Elleby B, Norstrom C, Stefansson K, Abrahmsen L, Oppermann U, Svensson S J Biol Chem. 2005 Feb 4;280(5):3789-94. Epub 2004 Nov 12. PMID:15542590<ref>PMID:15542590</ref>

-	~~===Crystal Structure~~ of ~~Guinea Pig 11beta-Hydroxysteroid Dehydrogenase Type 1===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_15542590}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[1xse]] is a 2 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Cavia_porcellus Cavia porcellus]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=1XSE OCA].~~	+

	==See Also==		==See Also==
	*[[Hydroxysteroid dehydrogenase\|Hydroxysteroid dehydrogenase]]		*[[Hydroxysteroid dehydrogenase\|Hydroxysteroid dehydrogenase]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	<~~ref group="xtra">PMID:015542590<~~/~~ref~~><~~references group="xtra"~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: 11-beta-hydroxysteroid dehydrogenase]]		[[Category: 11-beta-hydroxysteroid dehydrogenase]]
	[[Category: Cavia porcellus]]		[[Category: Cavia porcellus]]

OCA at 21:37, 20 October 2012

OCA — Sat, 20 Oct 2012 21:37:35 GMT

←Older revision		Revision as of 21:37, 20 October 2012
Line 8:		Line 8:

	==About this Structure==		==About this Structure==
-	[[1xse]] is a 2 chain structure ~~of [[Hydroxysteroid dehydrogenase]]~~ with sequence from [http://en.wikipedia.org/wiki/Cavia_porcellus Cavia porcellus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XSE OCA].	+	[[1xse]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Cavia_porcellus Cavia porcellus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XSE OCA].

	==See Also==		==See Also==

OCA at 13:47, 27 July 2012

OCA — Fri, 27 Jul 2012 13:47:45 GMT

←Older revision		Revision as of 13:47, 27 July 2012
Line 1:		Line 1:
	[[Image:1xse.png\|left\|200px]]		[[Image:1xse.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_1xse", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_1xse\| PDB=1xse \| SCENE= }}		{{STRUCTURE_1xse\| PDB=1xse \| SCENE= }}

	===Crystal Structure of Guinea Pig 11beta-Hydroxysteroid Dehydrogenase Type 1===		===Crystal Structure of Guinea Pig 11beta-Hydroxysteroid Dehydrogenase Type 1===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_15542590}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 15542590 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_15542590}}		{{ABSTRACT_PUBMED_15542590}}

	==About this Structure==		==About this Structure==
-	[[1xse]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Cavia_porcellus Cavia porcellus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XSE OCA].	+	[[1xse]] is a 2 chain structure of [[Hydroxysteroid dehydrogenase]] with sequence from [http://en.wikipedia.org/wiki/Cavia_porcellus Cavia porcellus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XSE OCA].
		+
		+	==See Also==
		+	*[[Hydroxysteroid dehydrogenase\|Hydroxysteroid dehydrogenase]]

	==Reference==		==Reference==

OCA: Protected "1xse" [edit=sysop:move=sysop]

OCA — Wed, 22 Jun 2011 15:48:59 GMT

Protected "1xse" [edit=sysop:move=sysop]

←Older revision

Revision as of 15:48, 22 June 2011