1xss - Revision history

OCA at 08:11, 15 November 2023

OCA — Wed, 15 Nov 2023 08:11:29 GMT

←Older revision		Revision as of 08:11, 15 November 2023
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	<StructureSection load='1xss' size='340' side='right'caption='[[1xss]], [[Resolution\|resolution]] 1.60Å' scene=''>		<StructureSection load='1xss' size='340' side='right'caption='[[1xss]], [[Resolution\|resolution]] 1.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1xss]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Favfa Favfa~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XSS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1XSS FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1xss]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Dipsastraea_favus Dipsastraea favus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XSS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1XSS FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>~~, ~~<scene name='pdbligand=NA:SODIUM+ION'>NA</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.6Å</td></tr>
-	<tr id='~~NonStdRes~~'><td class="sblockLbl"><b>[[~~Non-Standard_Residue~~\|~~NonStd Res~~:]]</b></td><td class="sblockDat"><scene name='pdbligand=DYG:(3S)-3-AMINO-3-[(4Z)-1-(CARBOXYMETHYL)-4-[(4-HYDROXYPHENYL)METHYLIDENE]-5-OXO-IMIDAZOL-2-YL]PROPANOIC+ACID'>DYG</scene></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DYG:(3S)-3-AMINO-3-[(4Z)-1-(CARBOXYMETHYL)-4-[(4-HYDROXYPHENYL)METHYLIDENE]-5-OXO-IMIDAZOL-2-YL]PROPANOIC+ACID'>DYG</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1xss FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1xss OCA], [https://pdbe.org/1xss PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1xss RCSB], [https://www.ebi.ac.uk/pdbsum/1xss PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1xss ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1xss FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1xss OCA], [https://pdbe.org/1xss PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1xss RCSB], [https://www.ebi.ac.uk/pdbsum/1xss PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1xss ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/Q53UG7_DIPFA Q53UG7_DIPFA]
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Favfa~~]]	+	[[Category: Dipsastraea favus]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Karasawa, S]]	+	[[Category: Karasawa S]]
-	[[Category: Miyawaki, A]]	+	[[Category: Miyawaki A]]
-	[[Category: Nukina, N]]	+	[[Category: Nukina N]]
-	[[Category: Shimizu, H]]	+	[[Category: Shimizu H]]
-	[[Category: Tsutsui, H]]	+	[[Category: Tsutsui H]]
-	~~[[Category: Fluorescent protein]]~~	+
-	~~[[Category: Luminescent protein~~]]	+

OCA at 16:18, 3 November 2021

OCA — Wed, 03 Nov 2021 16:18:54 GMT

←Older revision		Revision as of 16:18, 3 November 2021
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	<StructureSection load='1xss' size='340' side='right'caption='[[1xss]], [[Resolution\|resolution]] 1.60Å' scene=''>		<StructureSection load='1xss' size='340' side='right'caption='[[1xss]], [[Resolution\|resolution]] 1.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1xss]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Favfa Favfa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XSS OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1XSS FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1xss]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Favfa Favfa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XSS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1XSS FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=DYG:(3S)-3-AMINO-3-[(4Z)-1-(CARBOXYMETHYL)-4-[(4-HYDROXYPHENYL)METHYLIDENE]-5-OXO-IMIDAZOL-2-YL]PROPANOIC+ACID'>DYG</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=DYG:(3S)-3-AMINO-3-[(4Z)-1-(CARBOXYMETHYL)-4-[(4-HYDROXYPHENYL)METHYLIDENE]-5-OXO-IMIDAZOL-2-YL]PROPANOIC+ACID'>DYG</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1xss FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1xss OCA], [~~http~~://pdbe.org/1xss PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1xss RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1xss PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1xss ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1xss FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1xss OCA], [https://pdbe.org/1xss PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1xss RCSB], [https://www.ebi.ac.uk/pdbsum/1xss PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1xss ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==

OCA at 17:13, 22 January 2020

OCA — Wed, 22 Jan 2020 17:13:43 GMT

←Older revision		Revision as of 17:13, 22 January 2020
Line 1:		Line 1:

	==Semi-rational engineering of a green-emitting coral fluorescent protein into an efficient highlighter.==		==Semi-rational engineering of a green-emitting coral fluorescent protein into an efficient highlighter.==
-	<StructureSection load='1xss' size='340' side='right' caption='[[1xss]], [[Resolution\|resolution]] 1.60Å' scene=''>	+	<StructureSection load='1xss' size='340' side='right'caption='[[1xss]], [[Resolution\|resolution]] 1.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1xss]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Favfa Favfa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XSS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1XSS FirstGlance]. <br>		<table><tr><td colspan='2'>[[1xss]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Favfa Favfa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XSS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1XSS FirstGlance]. <br>
Line 29:		Line 29:

	==See Also==		==See Also==
-	*[[Green Fluorescent Protein\|Green Fluorescent Protein]]	+	*[[Green Fluorescent Protein 3D structures\|Green Fluorescent Protein 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 35:		Line 35:
	</StructureSection>		</StructureSection>
	[[Category: Favfa]]		[[Category: Favfa]]
		+	[[Category: Large Structures]]
	[[Category: Karasawa, S]]		[[Category: Karasawa, S]]
	[[Category: Miyawaki, A]]		[[Category: Miyawaki, A]]

OCA at 06:58, 18 April 2018

OCA — Wed, 18 Apr 2018 06:58:00 GMT

←Older revision		Revision as of 06:58, 18 April 2018
Line 1:		Line 1:
		+
	==Semi-rational engineering of a green-emitting coral fluorescent protein into an efficient highlighter.==		==Semi-rational engineering of a green-emitting coral fluorescent protein into an efficient highlighter.==
	<StructureSection load='1xss' size='340' side='right' caption='[[1xss]], [[Resolution\|resolution]] 1.60Å' scene=''>		<StructureSection load='1xss' size='340' side='right' caption='[[1xss]], [[Resolution\|resolution]] 1.60Å' scene=''>
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	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=DYG:(3S)-3-AMINO-3-[(4Z)-1-(CARBOXYMETHYL)-4-[(4-HYDROXYPHENYL)METHYLIDENE]-5-OXO-IMIDAZOL-2-YL]PROPANOIC+ACID'>DYG</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=DYG:(3S)-3-AMINO-3-[(4Z)-1-(CARBOXYMETHYL)-4-[(4-HYDROXYPHENYL)METHYLIDENE]-5-OXO-IMIDAZOL-2-YL]PROPANOIC+ACID'>DYG</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1xss FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1xss OCA], [http://pdbe.org/1xss PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1xss RCSB], [http://www.ebi.ac.uk/pdbsum/1xss PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1xss FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1xss OCA], [http://pdbe.org/1xss PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1xss RCSB], [http://www.ebi.ac.uk/pdbsum/1xss PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1xss ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
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	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/xs/1xss_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/xs/1xss_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>

OCA at 08:52, 8 February 2016

OCA — Mon, 08 Feb 2016 08:52:29 GMT

←Older revision		Revision as of 08:52, 8 February 2016
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	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1xss ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 09:55, 10 September 2015

OCA — Thu, 10 Sep 2015 09:55:22 GMT

←Older revision		Revision as of 09:55, 10 September 2015
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	<StructureSection load='1xss' size='340' side='right' caption='[[1xss]], [[Resolution\|resolution]] 1.60Å' scene=''>		<StructureSection load='1xss' size='340' side='right' caption='[[1xss]], [[Resolution\|resolution]] 1.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1xss]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Favia_favus Favia favus~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XSS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1XSS FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1xss]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Favfa Favfa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XSS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1XSS FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=DYG:(3S)-3-AMINO-3-[(4Z)-1-(CARBOXYMETHYL)-4-[(4-HYDROXYPHENYL)METHYLIDENE]-5-OXO-IMIDAZOL-2-YL]PROPANOIC+ACID'>DYG</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=DYG:(3S)-3-AMINO-3-[(4Z)-1-(CARBOXYMETHYL)-4-[(4-HYDROXYPHENYL)METHYLIDENE]-5-OXO-IMIDAZOL-2-YL]PROPANOIC+ACID'>DYG</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1xss FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1xss OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1xss RCSB], [http://www.ebi.ac.uk/pdbsum/1xss PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1xss FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1xss OCA], [http://pdbe.org/1xss PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1xss RCSB], [http://www.ebi.ac.uk/pdbsum/1xss PDBsum]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
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	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1xss" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 32:		Line 33:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Favia favus~~]]	+	[[Category: Favfa]]
	[[Category: Karasawa, S]]		[[Category: Karasawa, S]]
	[[Category: Miyawaki, A]]		[[Category: Miyawaki, A]]

OCA at 08:53, 8 January 2015

OCA — Thu, 08 Jan 2015 08:53:13 GMT

←Older revision		Revision as of 08:53, 8 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1xss]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Favia_favus Favia favus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XSS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1XSS FirstGlance]. <br>		<table><tr><td colspan='2'>[[1xss]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Favia_favus Favia favus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XSS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1XSS FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=DYG:(3S)-3-AMINO-3-[(4Z)-1-(CARBOXYMETHYL)-4-[(4-HYDROXYPHENYL)METHYLIDENE]-5-OXO-IMIDAZOL-2-YL]PROPANOIC+ACID'>DYG</scene></td></tr>	+	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=DYG:(3S)-3-AMINO-3-[(4Z)-1-(CARBOXYMETHYL)-4-[(4-HYDROXYPHENYL)METHYLIDENE]-5-OXO-IMIDAZOL-2-YL]PROPANOIC+ACID'>DYG</scene></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1xss FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1xss OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1xss RCSB], [http://www.ebi.ac.uk/pdbsum/1xss PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1xss FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1xss OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1xss RCSB], [http://www.ebi.ac.uk/pdbsum/1xss PDBsum]</span></td></tr>
-	<table>	+	</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	</StructureSection>		</StructureSection>
	[[Category: Favia favus]]		[[Category: Favia favus]]
-	[[Category: Karasawa, S.]]	+	[[Category: Karasawa, S]]
-	[[Category: Miyawaki, A.]]	+	[[Category: Miyawaki, A]]
-	[[Category: Nukina, N.]]	+	[[Category: Nukina, N]]
-	[[Category: Shimizu, H.]]	+	[[Category: Shimizu, H]]
-	[[Category: Tsutsui, H.]]	+	[[Category: Tsutsui, H]]
	[[Category: Fluorescent protein]]		[[Category: Fluorescent protein]]
	[[Category: Luminescent protein]]		[[Category: Luminescent protein]]

OCA at 05:13, 3 October 2014

OCA — Fri, 03 Oct 2014 05:13:22 GMT

←Older revision		Revision as of 05:13, 3 October 2014
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-	[[~~Image:~~1xss.~~png~~\|~~left~~\|200px]]	+	==Semi-rational engineering of a green-emitting coral fluorescent protein into an efficient highlighter.==
		+	<StructureSection load='1xss' size='340' side='right' caption='[[1xss]], [[Resolution\|resolution]] 1.60Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1xss]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Favia_favus Favia favus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XSS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1XSS FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=DYG:(3S)-3-AMINO-3-[(4Z)-1-(CARBOXYMETHYL)-4-[(4-HYDROXYPHENYL)METHYLIDENE]-5-OXO-IMIDAZOL-2-YL]PROPANOIC+ACID'>DYG</scene></td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1xss FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1xss OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1xss RCSB], [http://www.ebi.ac.uk/pdbsum/1xss PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/xs/1xss_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Kaede is a natural photoconvertible fluorescent protein found in the coral Trachyphyllia geoffroyi. It contains a tripeptide, His 62-Tyr 63-Gly 64, which acts as a green chromophore that is photoconvertible to red following (ultra-) violet irradiation. Here, we report the molecular cloning and crystal structure determination of a new fluorescent protein, KikG, from the coral Favia favus, and its in vitro evolution conferring green-to-red photoconvertibility. Substitution of the His 62-Tyr 63-Gly 64 sequence into the native protein provided only negligible photoconversion. On the basis of the crystal structure, semi-rational mutagenesis of the amino acids surrounding the chromophore was performed, leading to the generation of an efficient highlighter, KikGR. Within mammalian cells, KikGR is more efficiently photoconverted and is several-fold brighter in both the green and red states than Kaede. In addition, KikGR was successfully photoconverted using two-photon excitation microscopy at 760 nm, ensuring optical cell labelling with better spatial discrimination in thick and highly scattering tissues.

-	~~{{STRUCTURE_1xss\| PDB=1xss \| SCENE= }}~~	+	Semi-rational engineering of a coral fluorescent protein into an efficient highlighter.,Tsutsui H, Karasawa S, Shimizu H, Nukina N, Miyawaki A EMBO Rep. 2005 Mar;6(3):233-8. PMID:15731765<ref>PMID:15731765</ref>

-	~~===Semi-rational engineering~~ of ~~a green-emitting coral fluorescent protein into an efficient highlighter~~.~~===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_15731765}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[1xss]] is a 2 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org~~/~~wiki/Favia_favus Favia favus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XSS OCA].~~	+

	==See Also==		==See Also==
-	*[[Alyssa Marsico/Sandbox 1\|Alyssa Marsico/Sandbox 1]]
-	*[[Devon McCarthy/Sandbox 1\|Devon McCarthy/Sandbox 1]]
	*[[Green Fluorescent Protein\|Green Fluorescent Protein]]		*[[Green Fluorescent Protein\|Green Fluorescent Protein]]
-	*[[Sandbox104\|Sandbox104]]	+	== References ==
-	*[[User:Joanne Lau/Sandbox 5\|User:Joanne Lau/Sandbox 5]]	+	<references/>
-		+	__TOC__
-	==~~Reference~~==	+	</StructureSection>
-	<~~ref group="xtra">PMID:015731765<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Favia favus]]		[[Category: Favia favus]]
	[[Category: Karasawa, S.]]		[[Category: Karasawa, S.]]

OCA at 09:36, 20 October 2012

OCA — Sat, 20 Oct 2012 09:36:31 GMT

←Older revision		Revision as of 09:36, 20 October 2012
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	==About this Structure==		==About this Structure==
-	[[1xss]] is a 2 chain structure ~~of [[Alyssa Marsico/Sandbox 1]], [[Devon McCarthy/Sandbox 1]], [[Green Fluorescent Protein]], [[Sandbox104]] and [[User:Joanne Lau/Sandbox 5]]~~ with sequence from [http://en.wikipedia.org/wiki/Favia_favus Favia favus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XSS OCA].	+	[[1xss]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Favia_favus Favia favus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XSS OCA].

	==See Also==		==See Also==

OCA at 23:25, 25 July 2012

OCA — Wed, 25 Jul 2012 23:25:18 GMT

←Older revision		Revision as of 23:25, 25 July 2012
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	[[Image:1xss.png\|left\|200px]]		[[Image:1xss.png\|left\|200px]]

-	<!--
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-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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	{{STRUCTURE_1xss\| PDB=1xss \| SCENE= }}		{{STRUCTURE_1xss\| PDB=1xss \| SCENE= }}

	===Semi-rational engineering of a green-emitting coral fluorescent protein into an efficient highlighter.===		===Semi-rational engineering of a green-emitting coral fluorescent protein into an efficient highlighter.===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_15731765}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 15731765 is the PubMed ID number.
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	{{ABSTRACT_PUBMED_15731765}}		{{ABSTRACT_PUBMED_15731765}}

	==About this Structure==		==About this Structure==
-	[[1xss]] is a 2 chain structure of [[Green Fluorescent Protein]] with sequence from [http://en.wikipedia.org/wiki/Favia_favus Favia favus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XSS OCA].	+	[[1xss]] is a 2 chain structure of [[Alyssa Marsico/Sandbox 1]], [[Devon McCarthy/Sandbox 1]], [[Green Fluorescent Protein]], [[Sandbox104]] and [[User:Joanne Lau/Sandbox 5]] with sequence from [http://en.wikipedia.org/wiki/Favia_favus Favia favus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XSS OCA].

	==See Also==		==See Also==
-	*[[Green Fluorescent Protein]]	+	*[[Alyssa Marsico/Sandbox 1\|Alyssa Marsico/Sandbox 1]]
		+	*[[Devon McCarthy/Sandbox 1\|Devon McCarthy/Sandbox 1]]
		+	*[[Green Fluorescent Protein\|Green Fluorescent Protein]]
		+	*[[Sandbox104\|Sandbox104]]
		+	*[[User:Joanne Lau/Sandbox 5\|User:Joanne Lau/Sandbox 5]]

	==Reference==		==Reference==