1xx5 - Revision history

OCA at 08:09, 25 October 2023

OCA — Wed, 25 Oct 2023 08:09:34 GMT

←Older revision		Revision as of 08:09, 25 October 2023
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1xx5]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Naja_atra Naja atra]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XX5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1XX5 FirstGlance]. <br>		<table><tr><td colspan='2'>[[1xx5]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Naja_atra Naja atra]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XX5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1XX5 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EOH:ETHANOL'>EOH</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.4Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EOH:ETHANOL'>EOH</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1xx5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1xx5 OCA], [https://pdbe.org/1xx5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1xx5 RCSB], [https://www.ebi.ac.uk/pdbsum/1xx5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1xx5 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1xx5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1xx5 OCA], [https://pdbe.org/1xx5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1xx5 RCSB], [https://www.ebi.ac.uk/pdbsum/1xx5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1xx5 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/CRVP1_NAJAT CRVP1_NAJAT]] Inhibits calcium-activated potassium channels (KCa1.1/KCNMA1), voltage-gated potassium channel Kv1.3/KCNA3, and the calcium release channel/ryanodine receptor (RyR). Binds specifically to type 1 RyR (RyR1) from skeletal muscle. Inhibit both the binding of ryanodine to RyR1, and RyR1's calcium-channel activity. Inhibits carbachol-induced muscle contraction and weakly blocks muscle contraction evoked by potassium.<ref>PMID:15581679</ref> <ref>PMID:17070778</ref> <ref>PMID:18658224</ref> <ref>PMID:16042391</ref>	+	[https://www.uniprot.org/uniprot/CRVP1_NAJAT CRVP1_NAJAT] Inhibits calcium-activated potassium channels (KCa1.1/KCNMA1), voltage-gated potassium channel Kv1.3/KCNA3, and the calcium release channel/ryanodine receptor (RyR). Binds specifically to type 1 RyR (RyR1) from skeletal muscle. Inhibit both the binding of ryanodine to RyR1, and RyR1's calcium-channel activity. Inhibits carbachol-induced muscle contraction and weakly blocks muscle contraction evoked by potassium.<ref>PMID:15581679</ref> <ref>PMID:17070778</ref> <ref>PMID:18658224</ref> <ref>PMID:16042391</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 34:		Line 35:
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Naja atra]]		[[Category: Naja atra]]
-	[[Category: Guo, M]]	+	[[Category: Guo M]]
-	[[Category: Lou~~, X H~~]]	+	[[Category: Lou XH]]
-	[[Category: Niu~~, L W~~]]	+	[[Category: Niu LW]]
-	[[Category: Shen, B]]	+	[[Category: Shen B]]
-	[[Category: Teng~~, M K~~]]	+	[[Category: Teng MK]]
-	[[Category: Wang, J]]	+	[[Category: Wang J]]
-	~~[[Category: Crisp]]~~	+
-	~~[[Category: Natrin]]~~	+
-	~~[[Category: Toxin~~]]	+

OCA at 16:21, 3 November 2021

OCA — Wed, 03 Nov 2021 16:21:05 GMT

←Older revision		Revision as of 16:21, 3 November 2021
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	<StructureSection load='1xx5' size='340' side='right'caption='[[1xx5]], [[Resolution\|resolution]] 2.40Å' scene=''>		<StructureSection load='1xx5' size='340' side='right'caption='[[1xx5]], [[Resolution\|resolution]] 2.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1xx5]] is a 3 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Naja_atra Naja atra]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XX5 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1XX5 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1xx5]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Naja_atra Naja atra]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XX5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1XX5 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EOH:ETHANOL'>EOH</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EOH:ETHANOL'>EOH</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1xx5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1xx5 OCA], [~~http~~://pdbe.org/1xx5 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1xx5 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1xx5 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1xx5 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1xx5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1xx5 OCA], [https://pdbe.org/1xx5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1xx5 RCSB], [https://www.ebi.ac.uk/pdbsum/1xx5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1xx5 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/CRVP1_NAJAT CRVP1_NAJAT]] Inhibits calcium-activated potassium channels (KCa1.1/KCNMA1), voltage-gated potassium channel Kv1.3/KCNA3, and the calcium release channel/ryanodine receptor (RyR). Binds specifically to type 1 RyR (RyR1) from skeletal muscle. Inhibit both the binding of ryanodine to RyR1, and RyR1's calcium-channel activity. Inhibits carbachol-induced muscle contraction and weakly blocks muscle contraction evoked by potassium.<ref>PMID:15581679</ref> <ref>PMID:17070778</ref> <ref>PMID:18658224</ref> <ref>PMID:16042391</ref>	+	[[https://www.uniprot.org/uniprot/CRVP1_NAJAT CRVP1_NAJAT]] Inhibits calcium-activated potassium channels (KCa1.1/KCNMA1), voltage-gated potassium channel Kv1.3/KCNA3, and the calcium release channel/ryanodine receptor (RyR). Binds specifically to type 1 RyR (RyR1) from skeletal muscle. Inhibit both the binding of ryanodine to RyR1, and RyR1's calcium-channel activity. Inhibits carbachol-induced muscle contraction and weakly blocks muscle contraction evoked by potassium.<ref>PMID:15581679</ref> <ref>PMID:17070778</ref> <ref>PMID:18658224</ref> <ref>PMID:16042391</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 16:54, 29 January 2020

OCA — Wed, 29 Jan 2020 16:54:21 GMT

←Older revision		Revision as of 16:54, 29 January 2020
Line 1:		Line 1:

	==Crystal Structure of Natrin from Naja atra snake venom==		==Crystal Structure of Natrin from Naja atra snake venom==
-	<StructureSection load='1xx5' size='340' side='right' caption='[[1xx5]], [[Resolution\|resolution]] 2.40Å' scene=''>	+	<StructureSection load='1xx5' size='340' side='right'caption='[[1xx5]], [[Resolution\|resolution]] 2.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1xx5]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Naja_atra Naja atra]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XX5 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1XX5 FirstGlance]. <br>		<table><tr><td colspan='2'>[[1xx5]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Naja_atra Naja atra]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XX5 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1XX5 FirstGlance]. <br>
Line 32:		Line 32:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Naja atra]]		[[Category: Naja atra]]
	[[Category: Guo, M]]		[[Category: Guo, M]]

OCA at 07:12, 18 April 2018

OCA — Wed, 18 Apr 2018 07:12:43 GMT

←Older revision		Revision as of 07:12, 18 April 2018
Line 1:		Line 1:
		+
	==Crystal Structure of Natrin from Naja atra snake venom==		==Crystal Structure of Natrin from Naja atra snake venom==
	<StructureSection load='1xx5' size='340' side='right' caption='[[1xx5]], [[Resolution\|resolution]] 2.40Å' scene=''>		<StructureSection load='1xx5' size='340' side='right' caption='[[1xx5]], [[Resolution\|resolution]] 2.40Å' scene=''>
Line 4:		Line 5:
	<table><tr><td colspan='2'>[[1xx5]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Naja_atra Naja atra]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XX5 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1XX5 FirstGlance]. <br>		<table><tr><td colspan='2'>[[1xx5]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Naja_atra Naja atra]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XX5 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1XX5 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EOH:ETHANOL'>EOH</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EOH:ETHANOL'>EOH</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1xx5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1xx5 OCA], [http://pdbe.org/1xx5 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1xx5 RCSB], [http://www.ebi.ac.uk/pdbsum/1xx5 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1xx5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1xx5 OCA], [http://pdbe.org/1xx5 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1xx5 RCSB], [http://www.ebi.ac.uk/pdbsum/1xx5 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1xx5 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 12:		Line 13:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/xx/1xx5_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/xx/1xx5_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>

OCA at 23:24, 8 February 2016

OCA — Mon, 08 Feb 2016 23:24:15 GMT

←Older revision		Revision as of 23:24, 8 February 2016
Line 16:		Line 16:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1xx5 ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 16:21, 10 September 2015

OCA — Thu, 10 Sep 2015 16:21:56 GMT

←Older revision		Revision as of 16:21, 10 September 2015
Line 4:		Line 4:
	<table><tr><td colspan='2'>[[1xx5]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Naja_atra Naja atra]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XX5 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1XX5 FirstGlance]. <br>		<table><tr><td colspan='2'>[[1xx5]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Naja_atra Naja atra]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XX5 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1XX5 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EOH:ETHANOL'>EOH</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EOH:ETHANOL'>EOH</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1xx5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1xx5 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1xx5 RCSB], [http://www.ebi.ac.uk/pdbsum/1xx5 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1xx5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1xx5 OCA], [http://pdbe.org/1xx5 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1xx5 RCSB], [http://www.ebi.ac.uk/pdbsum/1xx5 PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 26:		Line 26:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1xx5" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 02:51, 25 December 2014

OCA — Thu, 25 Dec 2014 02:51:32 GMT

←Older revision		Revision as of 02:51, 25 December 2014
Line 6:		Line 6:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1xx5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1xx5 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1xx5 RCSB], [http://www.ebi.ac.uk/pdbsum/1xx5 PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1xx5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1xx5 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1xx5 RCSB], [http://www.ebi.ac.uk/pdbsum/1xx5 PDBsum]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/CRVP1_NAJAT CRVP1_NAJAT]] Inhibits calcium-activated potassium channels (KCa1.1/KCNMA1), voltage-gated potassium channel Kv1.3/KCNA3, and the calcium release channel/ryanodine receptor (RyR). Binds specifically to type 1 RyR (RyR1) from skeletal muscle. Inhibit both the binding of ryanodine to RyR1, and RyR1's calcium-channel activity. Inhibits carbachol-induced muscle contraction and weakly blocks muscle contraction evoked by potassium.<ref>PMID:15581679</ref> <ref>PMID:17070778</ref> <ref>PMID:18658224</ref> <ref>PMID:16042391</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 07:43, 3 December 2014

OCA — Wed, 03 Dec 2014 07:43:28 GMT

←Older revision		Revision as of 07:43, 3 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1xx5]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Naja_atra Naja atra]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XX5 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1XX5 FirstGlance]. <br>		<table><tr><td colspan='2'>[[1xx5]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Naja_atra Naja atra]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XX5 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1XX5 FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EOH:ETHANOL'>EOH</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EOH:ETHANOL'>EOH</scene></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1xx5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1xx5 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1xx5 RCSB], [http://www.ebi.ac.uk/pdbsum/1xx5 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1xx5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1xx5 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1xx5 RCSB], [http://www.ebi.ac.uk/pdbsum/1xx5 PDBsum]</span></td></tr>
-	<table>	+	</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 18:		Line 18:
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
-	Cysteine-rich secretory proteins (CRISPs) are ~~widely distributed~~ in ~~mammals and~~ snake venoms. ~~They possess apparent homology but varying functions. The structure~~ of CRISPs ~~has remained elusive~~. ~~Two novel members of the family~~, natrin ~~and stecrisp, have been~~ purified from Naja atra ~~and Trimeresurus stejnegeri venoms~~, ~~respectively~~. ~~Their crystals diffract X~~-~~rays to resolution limits~~ of 2.1 and 1.~~6 angstroms~~, ~~respectively, and belong~~ to ~~the orthorhombic system with different space groups~~, ~~unit~~-~~cell parameters and numbers~~ of ~~molecules per asymmetric unit~~. ~~Their structures will contribute a structural~~ basis ~~for further functional~~ studies of ~~this family~~.	+	Cysteine-rich secretory proteins (CRISPs) are widespread in snake venoms. Some members of these CRISPs recently have been found to block L-type Ca(2+) channels or cyclic nucleotide-gated ion (CNG) channels. Here, natrin purified from Naja atra venom, a member of the CRISP family, can induce a further contractile response in the endothelium-denuded thoracic aorta of mouse which has been contracted by a high-K(+) solution. Further experiments show it can block the high-conductance calcium-activated potassium (BK(Ca)) channel in a concentration-dependent manner with an IC(50) of 34.4 nM and a Hill coefficient of 1.02, which suggests that only a single natrin molecule is required to bind an ion channel to block BK(Ca) current. The crystal structure of natrin displaying two domains in tandem shows its cysteine-rich domain (CRD) has relatively independent flexibility, especially for the C-terminal long loop (loop I) of CRD to participate in the interface of two domains. On the basis of previous studies of CNG channel and L-Ca(2+) channel blockers, and the sequence and structural comparison of natrin and stecrisp, the deviation of the vital loop I of CRD is suggested to contribute to different effects of some CRISPs in protein-protein interaction.

-	~~Purification, partial characterization, crystallization~~ and ~~preliminary X-ray diffraction~~ of ~~two~~ cysteine-rich secretory ~~proteins~~ from Naja atra ~~and Trimeresurus stejnegeri venoms~~.~~,Tu X~~, Wang J, Guo M, ~~Zheng D~~, Teng M, Niu L, Liu Q, Huang Q, Hao Q ~~Acta Crystallogr D Biol Crystallogr~~. ~~2004 Jun~~;60(~~Pt 6~~):~~1108~~-~~11. Epub 2004, May 21~~. PMID:~~15159571~~<ref>PMID:~~15159571~~</ref>	+	Blocking effect and crystal structure of natrin toxin, a cysteine-rich secretory protein from Naja atra venom that targets the BKCa channel.,Wang J, Shen B, Guo M, Lou X, Duan Y, Cheng XP, Teng M, Niu L, Liu Q, Huang Q, Hao Q Biochemistry. 2005 Aug 2;44(30):10145-52. PMID:16042391<ref>PMID:16042391</ref>

	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
Line 29:		Line 29:
	</StructureSection>		</StructureSection>
	[[Category: Naja atra]]		[[Category: Naja atra]]
-	[[Category: Guo, M.]]	+	[[Category: Guo, M]]
-	[[Category: Lou, X H.]]	+	[[Category: Lou, X H]]
-	[[Category: Niu, L W.]]	+	[[Category: Niu, L W]]
-	[[Category: Shen, B.]]	+	[[Category: Shen, B]]
-	[[Category: Teng, M K.]]	+	[[Category: Teng, M K]]
-	[[Category: Wang, J.]]	+	[[Category: Wang, J]]
	[[Category: Crisp]]		[[Category: Crisp]]
-	[[Category: Naja atra]]
	[[Category: Natrin]]		[[Category: Natrin]]
	[[Category: Toxin]]		[[Category: Toxin]]

OCA at 18:31, 29 September 2014

OCA — Mon, 29 Sep 2014 18:31:03 GMT

←Older revision		Revision as of 18:31, 29 September 2014
Line 1:		Line 1:
-	[[~~Image~~:1xx5.~~png\|left~~\|200px]]	+	==Crystal Structure of Natrin from Naja atra snake venom==
		+	<StructureSection load='1xx5' size='340' side='right' caption='[[1xx5]], [[Resolution\|resolution]] 2.40Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1xx5]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Naja_atra Naja atra]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XX5 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1XX5 FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EOH:ETHANOL'>EOH</scene><br>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1xx5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1xx5 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1xx5 RCSB], [http://www.ebi.ac.uk/pdbsum/1xx5 PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/xx/1xx5_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Cysteine-rich secretory proteins (CRISPs) are widely distributed in mammals and snake venoms. They possess apparent homology but varying functions. The structure of CRISPs has remained elusive. Two novel members of the family, natrin and stecrisp, have been purified from Naja atra and Trimeresurus stejnegeri venoms, respectively. Their crystals diffract X-rays to resolution limits of 2.1 and 1.6 angstroms, respectively, and belong to the orthorhombic system with different space groups, unit-cell parameters and numbers of molecules per asymmetric unit. Their structures will contribute a structural basis for further functional studies of this family.

-	~~{{STRUCTURE_1xx5\| PDB=1xx5 \| SCENE= }}~~	+	Purification, partial characterization, crystallization and preliminary X-ray diffraction of two cysteine-rich secretory proteins from Naja atra and Trimeresurus stejnegeri venoms.,Tu X, Wang J, Guo M, Zheng D, Teng M, Niu L, Liu Q, Huang Q, Hao Q Acta Crystallogr D Biol Crystallogr. 2004 Jun;60(Pt 6):1108-11. Epub 2004, May 21. PMID:15159571<ref>PMID:15159571</ref>

-	~~===Crystal Structure~~ of ~~Natrin from Naja atra snake venom===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_15159571}}~~	+	== References ==
-		+	<references/>
-	~~==About this Structure==~~	+	__TOC__
-	~~[[1xx5]] is a 3 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Naja_atra Naja atra]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=1XX5 OCA].~~	+	</StructureSection>
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:015159571<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Naja atra]]		[[Category: Naja atra]]
	[[Category: Guo, M.]]		[[Category: Guo, M.]]

OCA: Protected "1xx5" [edit=sysop:move=sysop]

OCA — Sat, 05 Jan 2013 11:10:44 GMT

Protected "1xx5" [edit=sysop:move=sysop]

←Older revision

Revision as of 11:10, 5 January 2013