1y2v - Revision history

OCA at 13:37, 13 March 2024

OCA — Wed, 13 Mar 2024 13:37:34 GMT

←Older revision		Revision as of 13:37, 13 March 2024
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1y2v]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Agaricus_bisporus Agaricus bisporus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Y2V OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1Y2V FirstGlance]. <br>		<table><tr><td colspan='2'>[[1y2v]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Agaricus_bisporus Agaricus bisporus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Y2V OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1Y2V FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~"><~~scene name~~='~~pdbligand~~=~~SER~~:~~SERINE'~~>~~SER~~</~~scene~~>, <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene>, <scene name='pdbligand=NGA:N-ACETYL-D-GALACTOSAMINE'>NGA</scene><~~/td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.9Å</td></tr>
-	~~<tr id~~='~~related'><td class~~=~~"sblockLbl"><b>[[Related_structure\|Related~~:~~]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;~~'>~~[[1y2t\|1y2t]], [[1y2u\|1y2u]], [[1y2w\|1y2w]], [[1y2x\|1y2x]]~~</~~div~~></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene>, <scene name='pdbligand=NGA:N-ACETYL-D-GALACTOSAMINE'>NGA</scene>, <scene name='pdbligand=SER:SERINE'>SER</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1y2v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1y2v OCA], [https://pdbe.org/1y2v PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1y2v RCSB], [https://www.ebi.ac.uk/pdbsum/1y2v PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1y2v ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1y2v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1y2v OCA], [https://pdbe.org/1y2v PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1y2v RCSB], [https://www.ebi.ac.uk/pdbsum/1y2v PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1y2v ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/ABL_AGABI ABL_AGABI] Lectin that recognizes O-linked galactose-beta-1,3-N-acetylgalactosamine, a disaccharide (Thomsen-Friedenreich antigen or T-disaccharide), present on cell surface glycoproteins. Can also bind galactose-beta-1,3-N-acetylglucosamine. Does not bind monosaccharides. Can be internalized by clathrin-coated vesicles after binding to surface glycoproteins. After internalization it inhibits nuclear import of nuclear localization signal dependent proteins. Inhibits proliferation of malignant cells without cytotoxicity for normal cells.<ref>PMID:4343164</ref> <ref>PMID:8402638</ref> <ref>PMID:9988731</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1y2v ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1y2v ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	The lectin from the common mushroom Agaricus bisporus, the most popular edible species in Western countries, has potent antiproliferative effects on human epithelial cancer cells, without any apparent cytotoxicity. This property confers to it an important therapeutic potential as an antineoplastic agent. The three-dimensional structure of the lectin was determined by x-ray diffraction. The protein is a tetramer with 222 symmetry, and each monomer presents a novel fold with two beta sheets connected by a helix-loop-helix motif. Selectivity was studied by examining the binding of four monosaccharides and seven disaccharides in two different crystal forms. The T-antigen disaccharide, Galbeta1-3GalNAc, mediator of the antiproliferative effects of the protein, binds at a shallow depression on the surface of the molecule. The binding of N-acetylgalactosamine overlaps with that moiety of the T antigen, but surprisingly, N-acetylglucosamine, which differs from N-acetylgalactosamine only in the configuration of epimeric hydroxyl 4, binds at a totally different site on the opposite side of the helix-loop-helix motif. The lectin thus has two distinct binding sites per monomer that recognize the different configuration of a single epimeric hydroxyl. The structure of the protein and its two carbohydrate-binding sites are described in detail in this study.
-
-	The antineoplastic lectin of the common edible mushroom (Agaricus bisporus) has two binding sites, each specific for a different configuration at a single epimeric hydroxyl.,Carrizo ME, Capaldi S, Perduca M, Irazoqui FJ, Nores GA, Monaco HL J Biol Chem. 2005 Mar 18;280(11):10614-23. Epub 2004 Dec 13. PMID:15596442<ref>PMID:15596442</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 1y2v" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
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	[[Category: Agaricus bisporus]]		[[Category: Agaricus bisporus]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Capaldi, S]]	+	[[Category: Capaldi S]]
-	[[Category: Carrizo~~, M E~~]]	+	[[Category: Carrizo ME]]
-	[[Category: Irazoqui~~, F J~~]]	+	[[Category: Irazoqui FJ]]
-	[[Category: Monaco~~, H L~~]]	+	[[Category: Monaco HL]]
-	[[Category: Nores~~, G A~~]]	+	[[Category: Nores GA]]
-	[[Category: Perduca, M]]	+	[[Category: Perduca M]]
-	~~[[Category: Abl]]~~	+
-	~~[[Category: Lectin]]~~	+
-	~~[[Category: Mushroom]]~~	+
-	~~[[Category: Sugar binding protein]]~~	+
-	~~[[Category: T-antigen~~]]	+

OCA at 09:06, 21 April 2021

OCA — Wed, 21 Apr 2021 09:06:48 GMT

←Older revision		Revision as of 09:06, 21 April 2021
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	<StructureSection load='1y2v' size='340' side='right'caption='[[1y2v]], [[Resolution\|resolution]] 1.90Å' scene=''>		<StructureSection load='1y2v' size='340' side='right'caption='[[1y2v]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1y2v]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Agabi Agabi~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Y2V OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1Y2V FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1y2v]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Agaricus_bisporus Agaricus bisporus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Y2V OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1Y2V FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=~~GAL~~:~~BETA-D-GALACTOSE~~'>~~GAL~~</scene>, <scene name='pdbligand=~~NGA~~:~~N-ACETYL~~-D-~~GALACTOSAMINE~~'>~~NGA~~</scene>, <scene name='pdbligand=~~SER~~:~~SERINE~~'>~~SER~~</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SER:SERINE'>SER</scene>, <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene>, <scene name='pdbligand=NGA:N-ACETYL-D-GALACTOSAMINE'>NGA</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1y2t\|1y2t]], [[1y2u\|1y2u]], [[1y2w\|1y2w]], [[1y2x\|1y2x]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1y2t\|1y2t]], [[1y2u\|1y2u]], [[1y2w\|1y2w]], [[1y2x\|1y2x]]</div></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1y2v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1y2v OCA], [~~http~~://pdbe.org/1y2v PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1y2v RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1y2v PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1y2v ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1y2v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1y2v OCA], [https://pdbe.org/1y2v PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1y2v RCSB], [https://www.ebi.ac.uk/pdbsum/1y2v PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1y2v ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 31:		Line 31:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Agabi~~]]	+	[[Category: Agaricus bisporus]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Capaldi, S]]		[[Category: Capaldi, S]]
Line 40:		Line 40:
	[[Category: Perduca, M]]		[[Category: Perduca, M]]
	[[Category: Abl]]		[[Category: Abl]]
-	[[Category: Agaricus bisporus]]
	[[Category: Lectin]]		[[Category: Lectin]]
	[[Category: Mushroom]]		[[Category: Mushroom]]
	[[Category: Sugar binding protein]]		[[Category: Sugar binding protein]]
	[[Category: T-antigen]]		[[Category: T-antigen]]

OCA at 13:13, 17 July 2019

OCA — Wed, 17 Jul 2019 13:13:15 GMT

←Older revision		Revision as of 13:13, 17 July 2019
Line 1:		Line 1:

	==Crystal structure of the common edible mushroom (Agaricus bisporus) lectin in complex with T-antigen==		==Crystal structure of the common edible mushroom (Agaricus bisporus) lectin in complex with T-antigen==
-	<StructureSection load='1y2v' size='340' side='right' caption='[[1y2v]], [[Resolution\|resolution]] 1.90Å' scene=''>	+	<StructureSection load='1y2v' size='340' side='right'caption='[[1y2v]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1y2v]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Agabi Agabi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Y2V OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1Y2V FirstGlance]. <br>		<table><tr><td colspan='2'>[[1y2v]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Agabi Agabi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Y2V OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1Y2V FirstGlance]. <br>
Line 12:		Line 12:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/y2/1y2v_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/y2/1y2v_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
Line 32:		Line 32:
	</StructureSection>		</StructureSection>
	[[Category: Agabi]]		[[Category: Agabi]]
		+	[[Category: Large Structures]]
	[[Category: Capaldi, S]]		[[Category: Capaldi, S]]
	[[Category: Carrizo, M E]]		[[Category: Carrizo, M E]]

OCA at 10:53, 12 October 2017

OCA — Thu, 12 Oct 2017 10:53:37 GMT

←Older revision		Revision as of 10:53, 12 October 2017
Line 1:		Line 1:
		+
	==Crystal structure of the common edible mushroom (Agaricus bisporus) lectin in complex with T-antigen==		==Crystal structure of the common edible mushroom (Agaricus bisporus) lectin in complex with T-antigen==
	<StructureSection load='1y2v' size='340' side='right' caption='[[1y2v]], [[Resolution\|resolution]] 1.90Å' scene=''>		<StructureSection load='1y2v' size='340' side='right' caption='[[1y2v]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1y2v]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Agaricus_bisporus Agaricus bisporus~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Y2V OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1Y2V FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1y2v]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Agabi Agabi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Y2V OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1Y2V FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=~~SER~~:~~SERINE~~'>~~SER~~</scene>, <scene name='pdbligand=~~GAL~~:~~BETA~~-D-~~GALACTOSE~~'>~~GAL~~</scene>, <scene name='pdbligand=~~NGA~~:~~N-ACETYL-D-GALACTOSAMINE~~'>~~NGA~~</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene>, <scene name='pdbligand=NGA:N-ACETYL-D-GALACTOSAMINE'>NGA</scene>, <scene name='pdbligand=SER:SERINE'>SER</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1y2t\|1y2t]], [[1y2u\|1y2u]], [[1y2w\|1y2w]], [[1y2x\|1y2x]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1y2t\|1y2t]], [[1y2u\|1y2u]], [[1y2w\|1y2w]], [[1y2x\|1y2x]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1y2v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1y2v OCA], [http://pdbe.org/1y2v PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1y2v RCSB], [http://www.ebi.ac.uk/pdbsum/1y2v PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1y2v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1y2v OCA], [http://pdbe.org/1y2v PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1y2v RCSB], [http://www.ebi.ac.uk/pdbsum/1y2v PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1y2v ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 30:		Line 31:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Agaricus bisporus~~]]	+	[[Category: Agabi]]
	[[Category: Capaldi, S]]		[[Category: Capaldi, S]]
	[[Category: Carrizo, M E]]		[[Category: Carrizo, M E]]
Line 38:		Line 39:
	[[Category: Perduca, M]]		[[Category: Perduca, M]]
	[[Category: Abl]]		[[Category: Abl]]
		+	[[Category: Agaricus bisporus]]
	[[Category: Lectin]]		[[Category: Lectin]]
	[[Category: Mushroom]]		[[Category: Mushroom]]
	[[Category: Sugar binding protein]]		[[Category: Sugar binding protein]]
	[[Category: T-antigen]]		[[Category: T-antigen]]

OCA at 14:54, 9 February 2016

OCA — Tue, 09 Feb 2016 14:54:58 GMT

←Older revision		Revision as of 14:54, 9 February 2016
Line 15:		Line 15:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1y2v ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 23:20, 10 September 2015

OCA — Thu, 10 Sep 2015 23:20:37 GMT

←Older revision		Revision as of 23:20, 10 September 2015
Line 5:		Line 5:
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SER:SERINE'>SER</scene>, <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene>, <scene name='pdbligand=NGA:N-ACETYL-D-GALACTOSAMINE'>NGA</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SER:SERINE'>SER</scene>, <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene>, <scene name='pdbligand=NGA:N-ACETYL-D-GALACTOSAMINE'>NGA</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1y2t\|1y2t]], [[1y2u\|1y2u]], [[1y2w\|1y2w]], [[1y2x\|1y2x]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1y2t\|1y2t]], [[1y2u\|1y2u]], [[1y2w\|1y2w]], [[1y2x\|1y2x]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1y2v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1y2v OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1y2v RCSB], [http://www.ebi.ac.uk/pdbsum/1y2v PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1y2v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1y2v OCA], [http://pdbe.org/1y2v PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1y2v RCSB], [http://www.ebi.ac.uk/pdbsum/1y2v PDBsum]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 25:		Line 25:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1y2v" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 10:12, 8 January 2015

OCA — Thu, 08 Jan 2015 10:12:47 GMT

←Older revision		Revision as of 10:12, 8 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1y2v]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Agaricus_bisporus Agaricus bisporus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Y2V OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1Y2V FirstGlance]. <br>		<table><tr><td colspan='2'>[[1y2v]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Agaricus_bisporus Agaricus bisporus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Y2V OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1Y2V FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SER:SERINE'>SER</scene>, <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene>, <scene name='pdbligand=NGA:N-ACETYL-D-GALACTOSAMINE'>NGA</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SER:SERINE'>SER</scene>, <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene>, <scene name='pdbligand=NGA:N-ACETYL-D-GALACTOSAMINE'>NGA</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1y2t\|1y2t]], [[1y2u\|1y2u]], [[1y2w\|1y2w]], [[1y2x\|1y2x]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1y2t\|1y2t]], [[1y2u\|1y2u]], [[1y2w\|1y2w]], [[1y2x\|1y2x]]</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1y2v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1y2v OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1y2v RCSB], [http://www.ebi.ac.uk/pdbsum/1y2v PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1y2v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1y2v OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1y2v RCSB], [http://www.ebi.ac.uk/pdbsum/1y2v PDBsum]</span></td></tr>
-	<table>	+	</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 30:		Line 30:
	</StructureSection>		</StructureSection>
	[[Category: Agaricus bisporus]]		[[Category: Agaricus bisporus]]
-	[[Category: Capaldi, S.]]	+	[[Category: Capaldi, S]]
-	[[Category: Carrizo, M E.]]	+	[[Category: Carrizo, M E]]
-	[[Category: Irazoqui, F J.]]	+	[[Category: Irazoqui, F J]]
-	[[Category: Monaco, H L.]]	+	[[Category: Monaco, H L]]
-	[[Category: Nores, G A.]]	+	[[Category: Nores, G A]]
-	[[Category: Perduca, M.]]	+	[[Category: Perduca, M]]
	[[Category: Abl]]		[[Category: Abl]]
-	[[Category: Agaricus bisporus]]
	[[Category: Lectin]]		[[Category: Lectin]]
	[[Category: Mushroom]]		[[Category: Mushroom]]
	[[Category: Sugar binding protein]]		[[Category: Sugar binding protein]]
	[[Category: T-antigen]]		[[Category: T-antigen]]

OCA at 19:01, 29 September 2014

OCA — Mon, 29 Sep 2014 19:01:01 GMT

←Older revision		Revision as of 19:01, 29 September 2014
Line 1:		Line 1:
-	[[~~Image:~~1y2v.~~png~~\|~~left~~\|200px]]	+	==Crystal structure of the common edible mushroom (Agaricus bisporus) lectin in complex with T-antigen==
		+	<StructureSection load='1y2v' size='340' side='right' caption='[[1y2v]], [[Resolution\|resolution]] 1.90Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1y2v]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Agaricus_bisporus Agaricus bisporus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Y2V OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1Y2V FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SER:SERINE'>SER</scene>, <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene>, <scene name='pdbligand=NGA:N-ACETYL-D-GALACTOSAMINE'>NGA</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1y2t\|1y2t]], [[1y2u\|1y2u]], [[1y2w\|1y2w]], [[1y2x\|1y2x]]</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1y2v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1y2v OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1y2v RCSB], [http://www.ebi.ac.uk/pdbsum/1y2v PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/y2/1y2v_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The lectin from the common mushroom Agaricus bisporus, the most popular edible species in Western countries, has potent antiproliferative effects on human epithelial cancer cells, without any apparent cytotoxicity. This property confers to it an important therapeutic potential as an antineoplastic agent. The three-dimensional structure of the lectin was determined by x-ray diffraction. The protein is a tetramer with 222 symmetry, and each monomer presents a novel fold with two beta sheets connected by a helix-loop-helix motif. Selectivity was studied by examining the binding of four monosaccharides and seven disaccharides in two different crystal forms. The T-antigen disaccharide, Galbeta1-3GalNAc, mediator of the antiproliferative effects of the protein, binds at a shallow depression on the surface of the molecule. The binding of N-acetylgalactosamine overlaps with that moiety of the T antigen, but surprisingly, N-acetylglucosamine, which differs from N-acetylgalactosamine only in the configuration of epimeric hydroxyl 4, binds at a totally different site on the opposite side of the helix-loop-helix motif. The lectin thus has two distinct binding sites per monomer that recognize the different configuration of a single epimeric hydroxyl. The structure of the protein and its two carbohydrate-binding sites are described in detail in this study.

-	~~{{STRUCTURE_1y2v\| PDB=1y2v \| SCENE= }}~~	+	The antineoplastic lectin of the common edible mushroom (Agaricus bisporus) has two binding sites, each specific for a different configuration at a single epimeric hydroxyl.,Carrizo ME, Capaldi S, Perduca M, Irazoqui FJ, Nores GA, Monaco HL J Biol Chem. 2005 Mar 18;280(11):10614-23. Epub 2004 Dec 13. PMID:15596442<ref>PMID:15596442</ref>

-	~~===Crystal structure~~ of the ~~common edible mushroom (Agaricus bisporus) lectin in complex with T-antigen===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_15596442}}~~	+	== References ==
-		+	<references/>
-	~~==About this Structure==~~	+	__TOC__
-	~~[[1y2v]] is a 2 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Agaricus_bisporus Agaricus bisporus]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=1Y2V OCA].~~	+	</StructureSection>
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:015596442<~~/~~ref~~><~~ref group="xtra">PMID:015039564</ref><references group="xtra"~~/>	+
	[[Category: Agaricus bisporus]]		[[Category: Agaricus bisporus]]
	[[Category: Capaldi, S.]]		[[Category: Capaldi, S.]]

OCA at 12:31, 23 January 2013

OCA — Wed, 23 Jan 2013 12:31:22 GMT

←Older revision		Revision as of 12:31, 23 January 2013
Line 1:		Line 1:
	[[Image:1y2v.png\|left\|200px]]		[[Image:1y2v.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_1y2v", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_1y2v\| PDB=1y2v \| SCENE= }}		{{STRUCTURE_1y2v\| PDB=1y2v \| SCENE= }}

	===Crystal structure of the common edible mushroom (Agaricus bisporus) lectin in complex with T-antigen===		===Crystal structure of the common edible mushroom (Agaricus bisporus) lectin in complex with T-antigen===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_15596442}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 15596442 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_15596442}}		{{ABSTRACT_PUBMED_15596442}}

Line 22:		Line 11:

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~15596442~~</ref><ref group="xtra">PMID:~~15039564~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:015596442</ref><ref group="xtra">PMID:015039564</ref><references group="xtra"/>
	[[Category: Agaricus bisporus]]		[[Category: Agaricus bisporus]]
	[[Category: Capaldi, S.]]		[[Category: Capaldi, S.]]

OCA: Protected "1y2v" [edit=sysop:move=sysop]

OCA — Mon, 14 Mar 2011 20:10:44 GMT

Protected "1y2v" [edit=sysop:move=sysop]

←Older revision

Revision as of 20:10, 14 March 2011