1y3f - Revision history

OCA at 06:52, 23 August 2023

OCA — Wed, 23 Aug 2023 06:52:06 GMT

←Older revision		Revision as of 06:52, 23 August 2023
Line 3:		Line 3:
	<StructureSection load='1y3f' size='340' side='right'caption='[[1y3f]], [[Resolution\|resolution]] 1.72Å' scene=''>		<StructureSection load='1y3f' size='340' side='right'caption='[[1y3f]], [[Resolution\|resolution]] 1.72Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1y3f]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~"bacillus_amyloliquifaciens"_(sic)_fukumoto_1943 "bacillus amyloliquifaciens" (sic) fukumoto 1943~~] and [https://en.wikipedia.org/wiki/~~Barley Barley~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Y3F OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1Y3F FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1y3f]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_amyloliquefaciens Bacillus amyloliquefaciens] and [https://en.wikipedia.org/wiki/Hordeum_vulgare Hordeum vulgare]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Y3F OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1Y3F FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=15P:POLYETHYLENE+GLYCOL+(N=34)'>15P</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CIT:CITRIC+ACID'>CIT</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene~~></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.72Å</td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1y1k\|1y1k]], [[1y33\|1y33]], [[1y34\|1y34]], [[1y3b\|1y3b]], [[1y3c\|1y3c]], [[1y3d\|1y3d]], [[1y48\|1y48]], [[1y4a\|1y4a]], [[1y4d\|1y4d]]</div></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=15P:POLYETHYLENE+GLYCOL+(N=34)'>15P</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CIT:CITRIC+ACID'>CIT</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">apr ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1390 "Bacillus amyloliquifaciens" (sic) Fukumoto 1943])</td></tr>	+
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Subtilisin Subtilisin], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.62 3.4.21.62] </span></td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1y3f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1y3f OCA], [https://pdbe.org/1y3f PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1y3f RCSB], [https://www.ebi.ac.uk/pdbsum/1y3f PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1y3f ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1y3f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1y3f OCA], [https://pdbe.org/1y3f PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1y3f RCSB], [https://www.ebi.ac.uk/pdbsum/1y3f PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1y3f ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/SUBT_BACAM SUBT_BACAM]] Subtilisin is an extracellular alkaline serine protease, it catalyzes the hydrolysis of proteins and peptide amides. Has a high substrate specificity to fibrin.<ref>PMID:12524032</ref>	+	[https://www.uniprot.org/uniprot/SUBT_BACAM SUBT_BACAM] Subtilisin is an extracellular alkaline serine protease, it catalyzes the hydrolysis of proteins and peptide amides. Has a high substrate specificity to fibrin.<ref>PMID:12524032</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Barley~~]]	+	[[Category: Bacillus amyloliquefaciens]]
		+	[[Category: Hordeum vulgare]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	~~[[Category: Subtilisin]]~~	+	[[Category: Koshland Jr DE]]
-	[[Category: Koshland~~, D E~~]]	+	[[Category: Kwan G]]
-	[[Category: Kwan, G]]	+	[[Category: Lu CJ]]
-	[[Category: Lu~~, C J~~]]	+	[[Category: Radisky ES]]
-	[[Category: Radisky~~, E S]]~~	+
-	~~[[Category: Hydrolase-hydrolase inhibitor complex]]~~	+
-	~~[[Category: Inhibitor]]~~	+
-	~~[[Category: Serine protease~~]]	+

OCA at 16:47, 20 October 2021

OCA — Wed, 20 Oct 2021 16:47:33 GMT

OCA at 10:32, 6 January 2021

OCA — Wed, 06 Jan 2021 10:32:20 GMT

←Older revision		Revision as of 10:32, 6 January 2021
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	==Crystal structure of the complex of subtilisin BPN' with chymotrypsin inhibitor 2 F69A mutant==		==Crystal structure of the complex of subtilisin BPN' with chymotrypsin inhibitor 2 F69A mutant==
-	<StructureSection load='1y3f' size='340' side='right' caption='[[1y3f]], [[Resolution\|resolution]] 1.72Å' scene=''>	+	<StructureSection load='1y3f' size='340' side='right'caption='[[1y3f]], [[Resolution\|resolution]] 1.72Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1y3f]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_amyloliquifaciens"_(sic)_fukumoto_1943 "bacillus amyloliquifaciens" (sic) fukumoto 1943] and [http://en.wikipedia.org/wiki/Barley Barley]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Y3F OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1Y3F FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1y3f]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_amyloliquifaciens"_(sic)_fukumoto_1943 "bacillus amyloliquifaciens" (sic) fukumoto 1943] and [http://en.wikipedia.org/wiki/Barley Barley]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Y3F OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=1Y3F FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=15P:POLYETHYLENE+GLYCOL+(N=34)'>15P</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CIT:CITRIC+ACID'>CIT</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=15P:POLYETHYLENE+GLYCOL+(N=34)'>15P</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CIT:CITRIC+ACID'>CIT</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1y1k\|1y1k]], [[1y33\|1y33]], [[1y34\|1y34]], [[1y3b\|1y3b]], [[1y3c\|1y3c]], [[1y3d\|1y3d]], [[1y48\|1y48]], [[1y4a\|1y4a]], [[1y4d\|1y4d]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1y1k\|1y1k]], [[1y33\|1y33]], [[1y34\|1y34]], [[1y3b\|1y3b]], [[1y3c\|1y3c]], [[1y3d\|1y3d]], [[1y48\|1y48]], [[1y4a\|1y4a]], [[1y4d\|1y4d]]</div></td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">apr ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1390 "Bacillus amyloliquifaciens" (sic) Fukumoto 1943])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">apr ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1390 "Bacillus amyloliquifaciens" (sic) Fukumoto 1943])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Subtilisin Subtilisin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.62 3.4.21.62] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Subtilisin Subtilisin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.62 3.4.21.62] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1y3f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1y3f OCA], [http://pdbe.org/1y3f PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1y3f RCSB], [http://www.ebi.ac.uk/pdbsum/1y3f PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1y3f ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=1y3f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1y3f OCA], [http://pdbe.org/1y3f PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1y3f RCSB], [http://www.ebi.ac.uk/pdbsum/1y3f PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1y3f ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 16:		Line 16:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/y3/1y3f_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/y3/1y3f_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
Line 31:		Line 31:
	</div>		</div>
	<div class="pdbe-citations 1y3f" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 1y3f" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Chymotrypsin inhibitor 3D structures\|Chymotrypsin inhibitor 3D structures]]
		+	*[[Subtilisin 3D structures\|Subtilisin 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 36:		Line 40:
	</StructureSection>		</StructureSection>
	[[Category: Barley]]		[[Category: Barley]]
		+	[[Category: Large Structures]]
	[[Category: Subtilisin]]		[[Category: Subtilisin]]
	[[Category: Koshland, D E]]		[[Category: Koshland, D E]]

OCA at 13:27, 12 October 2017

OCA — Thu, 12 Oct 2017 13:27:41 GMT

←Older revision		Revision as of 13:27, 12 October 2017
Line 1:		Line 1:
		+
	==Crystal structure of the complex of subtilisin BPN' with chymotrypsin inhibitor 2 F69A mutant==		==Crystal structure of the complex of subtilisin BPN' with chymotrypsin inhibitor 2 F69A mutant==
	<StructureSection load='1y3f' size='340' side='right' caption='[[1y3f]], [[Resolution\|resolution]] 1.72Å' scene=''>		<StructureSection load='1y3f' size='340' side='right' caption='[[1y3f]], [[Resolution\|resolution]] 1.72Å' scene=''>
Line 7:		Line 8:
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">apr ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1390 "Bacillus amyloliquifaciens" (sic) Fukumoto 1943])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">apr ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1390 "Bacillus amyloliquifaciens" (sic) Fukumoto 1943])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Subtilisin Subtilisin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.62 3.4.21.62] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Subtilisin Subtilisin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.62 3.4.21.62] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1y3f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1y3f OCA], [http://pdbe.org/1y3f PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1y3f RCSB], [http://www.ebi.ac.uk/pdbsum/1y3f PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1y3f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1y3f OCA], [http://pdbe.org/1y3f PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1y3f RCSB], [http://www.ebi.ac.uk/pdbsum/1y3f PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1y3f ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
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	</div>		</div>
	<div class="pdbe-citations 1y3f" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 1y3f" style="background-color:#fffaf0;"></div>
-
-	==See Also==
-	*[[Chymotrypsin Inhibitor\|Chymotrypsin Inhibitor]]
-	*[[Subtilisin\|Subtilisin]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 23:43, 9 February 2016

OCA — Tue, 09 Feb 2016 23:43:20 GMT

←Older revision		Revision as of 23:43, 9 February 2016
Line 19:		Line 19:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1y3f ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 03:14, 11 September 2015

OCA — Fri, 11 Sep 2015 03:14:12 GMT

←Older revision		Revision as of 03:14, 11 September 2015
Line 2:		Line 2:
	<StructureSection load='1y3f' size='340' side='right' caption='[[1y3f]], [[Resolution\|resolution]] 1.72Å' scene=''>		<StructureSection load='1y3f' size='340' side='right' caption='[[1y3f]], [[Resolution\|resolution]] 1.72Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1y3f]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Bacillus_amyloliquefaciens Bacillus amyloliquefaciens~~] and [http://en.wikipedia.org/wiki/~~Hordeum_vulgare Hordeum vulgare~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Y3F OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1Y3F FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1y3f]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_amyloliquifaciens"_(sic)_fukumoto_1943 "bacillus amyloliquifaciens" (sic) fukumoto 1943] and [http://en.wikipedia.org/wiki/Barley Barley]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Y3F OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1Y3F FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=15P:POLYETHYLENE+GLYCOL+(N=34)'>15P</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CIT:CITRIC+ACID'>CIT</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=15P:POLYETHYLENE+GLYCOL+(N=34)'>15P</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CIT:CITRIC+ACID'>CIT</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1y1k\|1y1k]], [[1y33\|1y33]], [[1y34\|1y34]], [[1y3b\|1y3b]], [[1y3c\|1y3c]], [[1y3d\|1y3d]], [[1y48\|1y48]], [[1y4a\|1y4a]], [[1y4d\|1y4d]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1y1k\|1y1k]], [[1y33\|1y33]], [[1y34\|1y34]], [[1y3b\|1y3b]], [[1y3c\|1y3c]], [[1y3d\|1y3d]], [[1y48\|1y48]], [[1y4a\|1y4a]], [[1y4d\|1y4d]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">apr ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1390 Bacillus ~~amyloliquefaciens~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">apr ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1390 "Bacillus amyloliquifaciens" (sic) Fukumoto 1943])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Subtilisin Subtilisin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.62 3.4.21.62] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Subtilisin Subtilisin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.62 3.4.21.62] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1y3f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1y3f OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1y3f RCSB], [http://www.ebi.ac.uk/pdbsum/1y3f PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1y3f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1y3f OCA], [http://pdbe.org/1y3f PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1y3f RCSB], [http://www.ebi.ac.uk/pdbsum/1y3f PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 29:		Line 29:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1y3f" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 37:		Line 38:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Bacillus amyloliquefaciens]]~~	+	[[Category: Barley]]
-	~~[[Category: Hordeum vulgare~~]]	+
	[[Category: Subtilisin]]		[[Category: Subtilisin]]
	[[Category: Koshland, D E]]		[[Category: Koshland, D E]]

OCA at 10:20, 25 December 2014

OCA — Thu, 25 Dec 2014 10:20:03 GMT

←Older revision		Revision as of 10:20, 25 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1y3f]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_amyloliquefaciens Bacillus amyloliquefaciens] and [http://en.wikipedia.org/wiki/Hordeum_vulgare Hordeum vulgare]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Y3F OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1Y3F FirstGlance]. <br>		<table><tr><td colspan='2'>[[1y3f]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_amyloliquefaciens Bacillus amyloliquefaciens] and [http://en.wikipedia.org/wiki/Hordeum_vulgare Hordeum vulgare]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Y3F OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1Y3F FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=15P:POLYETHYLENE+GLYCOL+(N=34)'>15P</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CIT:CITRIC+ACID'>CIT</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=15P:POLYETHYLENE+GLYCOL+(N=34)'>15P</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CIT:CITRIC+ACID'>CIT</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1y1k\|1y1k]], [[1y33\|1y33]], [[1y34\|1y34]], [[1y3b\|1y3b]], [[1y3c\|1y3c]], [[1y3d\|1y3d]], [[1y48\|1y48]], [[1y4a\|1y4a]], [[1y4d\|1y4d]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1y1k\|1y1k]], [[1y33\|1y33]], [[1y34\|1y34]], [[1y3b\|1y3b]], [[1y3c\|1y3c]], [[1y3d\|1y3d]], [[1y48\|1y48]], [[1y4a\|1y4a]], [[1y4d\|1y4d]]</td></tr>
-	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">apr ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1390 Bacillus amyloliquefaciens])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">apr ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1390 Bacillus amyloliquefaciens])</td></tr>
-	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Subtilisin Subtilisin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.62 3.4.21.62] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Subtilisin Subtilisin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.62 3.4.21.62] </span></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1y3f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1y3f OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1y3f RCSB], [http://www.ebi.ac.uk/pdbsum/1y3f PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1y3f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1y3f OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1y3f RCSB], [http://www.ebi.ac.uk/pdbsum/1y3f PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/SUBT_BACAM SUBT_BACAM]] Subtilisin is an extracellular alkaline serine protease, it catalyzes the hydrolysis of proteins and peptide amides. Has a high substrate specificity to fibrin.<ref>PMID:12524032</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 38:		Line 40:
	[[Category: Hordeum vulgare]]		[[Category: Hordeum vulgare]]
	[[Category: Subtilisin]]		[[Category: Subtilisin]]
-	[[Category: Koshland, D E.]]	+	[[Category: Koshland, D E]]
-	[[Category: Kwan, G.]]	+	[[Category: Kwan, G]]
-	[[Category: Lu, C J.]]	+	[[Category: Lu, C J]]
-	[[Category: Radisky, E S.]]	+	[[Category: Radisky, E S]]
	[[Category: Hydrolase-hydrolase inhibitor complex]]		[[Category: Hydrolase-hydrolase inhibitor complex]]
	[[Category: Inhibitor]]		[[Category: Inhibitor]]
	[[Category: Serine protease]]		[[Category: Serine protease]]

OCA at 23:21, 28 September 2014

OCA — Sun, 28 Sep 2014 23:21:35 GMT

←Older revision		Revision as of 23:21, 28 September 2014
Line 1:		Line 1:
-	[[~~Image:~~1y3f.~~png~~\|~~left~~\|200px]]	+	==Crystal structure of the complex of subtilisin BPN' with chymotrypsin inhibitor 2 F69A mutant==
		+	<StructureSection load='1y3f' size='340' side='right' caption='[[1y3f]], [[Resolution\|resolution]] 1.72Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1y3f]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_amyloliquefaciens Bacillus amyloliquefaciens] and [http://en.wikipedia.org/wiki/Hordeum_vulgare Hordeum vulgare]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Y3F OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1Y3F FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=15P:POLYETHYLENE+GLYCOL+(N=34)'>15P</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CIT:CITRIC+ACID'>CIT</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1y1k\|1y1k]], [[1y33\|1y33]], [[1y34\|1y34]], [[1y3b\|1y3b]], [[1y3c\|1y3c]], [[1y3d\|1y3d]], [[1y48\|1y48]], [[1y4a\|1y4a]], [[1y4d\|1y4d]]</td></tr>
		+	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">apr ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1390 Bacillus amyloliquefaciens])</td></tr>
		+	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Subtilisin Subtilisin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.62 3.4.21.62] </span></td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1y3f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1y3f OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1y3f RCSB], [http://www.ebi.ac.uk/pdbsum/1y3f PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/y3/1y3f_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	A series of mutants of chymotrypsin inhibitor 2 (CI2), at residues involved in intramolecular interactions that shape and constrain the binding loop, were studied to determine their relative importance for inhibition of the serine protease subtilisin BPN', and for resistance of the inhibitor to proteolysis. These functional properties were investigated in tandem with the crystal structures of the mutant inhibitor-enzyme complexes. A dense hydrogen bonding network that supports the binding loop in the vicinity of the scissile bond was found to be important both for enzyme affinity and for stability to proteolysis. Structural analysis, in combination with biochemical measurements, allows differentiation of the structural components most important for resistance to proteolysis and/or binding. The most critical participating residues in the network were found to be Thr-58, Glu-60, Arg-65, and Gly-83. Glu-60 is more important for resistance to proteolysis than for binding, while Arg-65 and two other Arg residues play a greater role in binding than in resistance to proteolysis. Structural comparisons reveal a wide variety of subtle conformational changes in response to mutation, with built-in robustness in the hydrogen bond network, such that loss of one contact is compensated by other new contacts.

-	~~{{STRUCTURE_1y3f\| PDB=1y3f \| SCENE= }}~~	+	Role of the intramolecular hydrogen bond network in the inhibitory power of chymotrypsin inhibitor 2.,Radisky ES, Lu CJ, Kwan G, Koshland DE Jr Biochemistry. 2005 May 10;44(18):6823-30. PMID:15865427<ref>PMID:15865427</ref>

-	~~===Crystal structure~~ of the ~~complex of subtilisin BPN' with chymotrypsin inhibitor 2 F69A mutant===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_15865427}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[1y3f]] is a 2 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Bacillus_amyloliquefaciens Bacillus amyloliquefaciens] and [http://en~~.~~wikipedia.org~~/~~wiki/Hordeum_vulgare Hordeum vulgare]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Y3F OCA].~~	+

	==See Also==		==See Also==
	*[[Chymotrypsin Inhibitor\|Chymotrypsin Inhibitor]]		*[[Chymotrypsin Inhibitor\|Chymotrypsin Inhibitor]]
	*[[Subtilisin\|Subtilisin]]		*[[Subtilisin\|Subtilisin]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	<~~ref group="xtra">PMID:015865427<~~/~~ref~~><~~references group="xtra"~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Bacillus amyloliquefaciens]]		[[Category: Bacillus amyloliquefaciens]]
	[[Category: Hordeum vulgare]]		[[Category: Hordeum vulgare]]

OCA at 15:33, 20 October 2012

OCA — Sat, 20 Oct 2012 15:33:20 GMT

←Older revision		Revision as of 15:33, 20 October 2012
Line 8:		Line 8:

	==About this Structure==		==About this Structure==
-	[[1y3f]] is a 2 chain structure ~~of [[Chymotrypsin Inhibitor]] and [[Subtilisin]]~~ with sequence from [http://en.wikipedia.org/wiki/Bacillus_amyloliquefaciens Bacillus amyloliquefaciens] and [http://en.wikipedia.org/wiki/Hordeum_vulgare Hordeum vulgare]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Y3F OCA].	+	[[1y3f]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_amyloliquefaciens Bacillus amyloliquefaciens] and [http://en.wikipedia.org/wiki/Hordeum_vulgare Hordeum vulgare]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Y3F OCA].

	==See Also==		==See Also==

OCA: Protected "1y3f" [edit=sysop:move=sysop]

OCA — Thu, 26 Jul 2012 18:44:36 GMT

Protected "1y3f" [edit=sysop:move=sysop]

←Older revision

Revision as of 18:44, 26 July 2012

←Older revision		Revision as of 16:47, 20 October 2021
Line 3:		Line 3:
	<StructureSection load='1y3f' size='340' side='right'caption='[[1y3f]], [[Resolution\|resolution]] 1.72Å' scene=''>		<StructureSection load='1y3f' size='340' side='right'caption='[[1y3f]], [[Resolution\|resolution]] 1.72Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1y3f]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/"bacillus_amyloliquifaciens"_(sic)_fukumoto_1943 "bacillus amyloliquifaciens" (sic) fukumoto 1943] and [~~http~~://en.wikipedia.org/wiki/Barley Barley]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Y3F OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=1Y3F FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1y3f]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/"bacillus_amyloliquifaciens"_(sic)_fukumoto_1943 "bacillus amyloliquifaciens" (sic) fukumoto 1943] and [https://en.wikipedia.org/wiki/Barley Barley]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Y3F OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1Y3F FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=15P:POLYETHYLENE+GLYCOL+(N=34)'>15P</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CIT:CITRIC+ACID'>CIT</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=15P:POLYETHYLENE+GLYCOL+(N=34)'>15P</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CIT:CITRIC+ACID'>CIT</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1y1k\|1y1k]], [[1y33\|1y33]], [[1y34\|1y34]], [[1y3b\|1y3b]], [[1y3c\|1y3c]], [[1y3d\|1y3d]], [[1y48\|1y48]], [[1y4a\|1y4a]], [[1y4d\|1y4d]]</div></td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1y1k\|1y1k]], [[1y33\|1y33]], [[1y34\|1y34]], [[1y3b\|1y3b]], [[1y3c\|1y3c]], [[1y3d\|1y3d]], [[1y48\|1y48]], [[1y4a\|1y4a]], [[1y4d\|1y4d]]</div></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">apr ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1390 "Bacillus amyloliquifaciens" (sic) Fukumoto 1943])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">apr ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1390 "Bacillus amyloliquifaciens" (sic) Fukumoto 1943])</td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Subtilisin Subtilisin], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.62 3.4.21.62] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Subtilisin Subtilisin], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.62 3.4.21.62] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=1y3f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1y3f OCA], [~~http~~://pdbe.org/1y3f PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1y3f RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1y3f PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1y3f ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1y3f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1y3f OCA], [https://pdbe.org/1y3f PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1y3f RCSB], [https://www.ebi.ac.uk/pdbsum/1y3f PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1y3f ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/SUBT_BACAM SUBT_BACAM]] Subtilisin is an extracellular alkaline serine protease, it catalyzes the hydrolysis of proteins and peptide amides. Has a high substrate specificity to fibrin.<ref>PMID:12524032</ref>	+	[[https://www.uniprot.org/uniprot/SUBT_BACAM SUBT_BACAM]] Subtilisin is an extracellular alkaline serine protease, it catalyzes the hydrolysis of proteins and peptide amides. Has a high substrate specificity to fibrin.<ref>PMID:12524032</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]