1y4e - Revision history

OCA at 09:30, 6 December 2023

OCA — Wed, 06 Dec 2023 09:30:32 GMT

←Older revision		Revision as of 09:30, 6 December 2023
Line 1:		Line 1:

	==NMR structure of transmembrane segment IV of the NHE1 isoform of the Na+/H+ exchanger==		==NMR structure of transmembrane segment IV of the NHE1 isoform of the Na+/H+ exchanger==
-	<StructureSection load='1y4e' size='340' side='right'caption='[[1y4e~~]], [[NMR_Ensembles_of_Models \| 100 NMR models~~]]' scene=''>	+	<StructureSection load='1y4e' size='340' side='right'caption='[[1y4e]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1y4e]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Human Human~~]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Y4E OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1Y4E FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1y4e]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Y4E OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1Y4E FirstGlance]. <br>
-	</td></tr><tr id='~~NonStdRes~~'><td class="sblockLbl"><b>[[~~Non-Standard_Residue~~\|~~NonStd Res~~:]]</b></td><td class="sblockDat"~~><scene name~~=~~'pdbligand=HSL:HOMOSERINE+LACTONE'>HSL</scene~~></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat">~~SLC9A1, APNH1, NHE1 ([https~~:/~~/www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HSL:HOMOSERINE+LACTONE'>HSL</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1y4e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1y4e OCA], [https://pdbe.org/1y4e PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1y4e RCSB], [https://www.ebi.ac.uk/pdbsum/1y4e PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1y4e ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1y4e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1y4e OCA], [https://pdbe.org/1y4e PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1y4e RCSB], [https://www.ebi.ac.uk/pdbsum/1y4e PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1y4e ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/SL9A1_HUMAN SL9A1_HUMAN]] Involved in pH regulation to eliminate acids generated by active metabolism or to counter adverse environmental conditions. Major proton extruding system driven by the inward sodium ion chemical gradient. Plays an important role in signal transduction.<ref>PMID:8901634</ref> <ref>PMID:11350981</ref> <ref>PMID:15035633</ref>	+	[https://www.uniprot.org/uniprot/SL9A1_HUMAN SL9A1_HUMAN] Involved in pH regulation to eliminate acids generated by active metabolism or to counter adverse environmental conditions. Major proton extruding system driven by the inward sodium ion chemical gradient. Plays an important role in signal transduction.<ref>PMID:8901634</ref> <ref>PMID:11350981</ref> <ref>PMID:15035633</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 23:		Line 23:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Human~~]]	+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Fliegel, L]]	+	[[Category: Fliegel L]]
-	[[Category: Li, X]]	+	[[Category: Li X]]
-	[[Category: Lindhout~~, D A~~]]	+	[[Category: Lindhout DA]]
-	[[Category: Liu, Y]]	+	[[Category: Liu Y]]
-	[[Category: Rainey~~, J K~~]]	+	[[Category: Rainey JK]]
-	[[Category: Slepkov~~, E R~~]]	+	[[Category: Slepkov ER]]
-	[[Category: Sykes~~, B D]]~~	+	[[Category: Sykes BD]]
-	~~[[Category: Membrane protein]]~~	+
-	~~[[Category: Nhe1 isoform]]~~	+
-	~~[[Category: Transmembrane~~]]	+

OCA at 07:11, 2 March 2022

OCA — Wed, 02 Mar 2022 07:11:16 GMT

←Older revision		Revision as of 07:11, 2 March 2022
Line 3:		Line 3:
	<StructureSection load='1y4e' size='340' side='right'caption='[[1y4e]], [[NMR_Ensembles_of_Models \| 100 NMR models]]' scene=''>		<StructureSection load='1y4e' size='340' side='right'caption='[[1y4e]], [[NMR_Ensembles_of_Models \| 100 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1y4e]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Human Human]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Y4E OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=1Y4E FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1y4e]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Human Human]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Y4E OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1Y4E FirstGlance]. <br>
	</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=HSL:HOMOSERINE+LACTONE'>HSL</scene></td></tr>		</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=HSL:HOMOSERINE+LACTONE'>HSL</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">SLC9A1, APNH1, NHE1 ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">SLC9A1, APNH1, NHE1 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=1y4e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1y4e OCA], [~~http~~://pdbe.org/1y4e PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1y4e RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1y4e PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1y4e ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1y4e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1y4e OCA], [https://pdbe.org/1y4e PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1y4e RCSB], [https://www.ebi.ac.uk/pdbsum/1y4e PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1y4e ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/SL9A1_HUMAN SL9A1_HUMAN]] Involved in pH regulation to eliminate acids generated by active metabolism or to counter adverse environmental conditions. Major proton extruding system driven by the inward sodium ion chemical gradient. Plays an important role in signal transduction.<ref>PMID:8901634</ref> <ref>PMID:11350981</ref> <ref>PMID:15035633</ref>	+	[[https://www.uniprot.org/uniprot/SL9A1_HUMAN SL9A1_HUMAN]] Involved in pH regulation to eliminate acids generated by active metabolism or to counter adverse environmental conditions. Major proton extruding system driven by the inward sodium ion chemical gradient. Plays an important role in signal transduction.<ref>PMID:8901634</ref> <ref>PMID:11350981</ref> <ref>PMID:15035633</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==

OCA at 10:33, 6 January 2021

OCA — Wed, 06 Jan 2021 10:33:22 GMT

←Older revision		Revision as of 10:33, 6 January 2021
Line 1:		Line 1:

	==NMR structure of transmembrane segment IV of the NHE1 isoform of the Na+/H+ exchanger==		==NMR structure of transmembrane segment IV of the NHE1 isoform of the Na+/H+ exchanger==
-	<StructureSection load='1y4e' size='340' side='right' caption='[[1y4e]], [[NMR_Ensembles_of_Models \| 100 NMR models]]' scene=''>	+	<StructureSection load='1y4e' size='340' side='right'caption='[[1y4e]], [[NMR_Ensembles_of_Models \| 100 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1y4e]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Y4E OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1Y4E FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1y4e]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Y4E OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=1Y4E FirstGlance]. <br>
	</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=HSL:HOMOSERINE+LACTONE'>HSL</scene></td></tr>		</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=HSL:HOMOSERINE+LACTONE'>HSL</scene></td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">SLC9A1, APNH1, NHE1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">SLC9A1, APNH1, NHE1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1y4e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1y4e OCA], [http://pdbe.org/1y4e PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1y4e RCSB], [http://www.ebi.ac.uk/pdbsum/1y4e PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1y4e ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=1y4e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1y4e OCA], [http://pdbe.org/1y4e PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1y4e RCSB], [http://www.ebi.ac.uk/pdbsum/1y4e PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1y4e ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 24:		Line 24:
	</StructureSection>		</StructureSection>
	[[Category: Human]]		[[Category: Human]]
		+	[[Category: Large Structures]]
	[[Category: Fliegel, L]]		[[Category: Fliegel, L]]
	[[Category: Li, X]]		[[Category: Li, X]]

OCA at 06:54, 18 April 2018

OCA — Wed, 18 Apr 2018 06:54:43 GMT

←Older revision		Revision as of 06:54, 18 April 2018
Line 1:		Line 1:
		+
	==NMR structure of transmembrane segment IV of the NHE1 isoform of the Na+/H+ exchanger==		==NMR structure of transmembrane segment IV of the NHE1 isoform of the Na+/H+ exchanger==
	<StructureSection load='1y4e' size='340' side='right' caption='[[1y4e]], [[NMR_Ensembles_of_Models \| 100 NMR models]]' scene=''>		<StructureSection load='1y4e' size='340' side='right' caption='[[1y4e]], [[NMR_Ensembles_of_Models \| 100 NMR models]]' scene=''>
Line 5:		Line 6:
	</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=HSL:HOMOSERINE+LACTONE'>HSL</scene></td></tr>		</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=HSL:HOMOSERINE+LACTONE'>HSL</scene></td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">SLC9A1, APNH1, NHE1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">SLC9A1, APNH1, NHE1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1y4e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1y4e OCA], [http://pdbe.org/1y4e PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1y4e RCSB], [http://www.ebi.ac.uk/pdbsum/1y4e PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1y4e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1y4e OCA], [http://pdbe.org/1y4e PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1y4e RCSB], [http://www.ebi.ac.uk/pdbsum/1y4e PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1y4e ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==

OCA at 09:04, 10 September 2015

OCA — Thu, 10 Sep 2015 09:04:24 GMT

←Older revision		Revision as of 09:04, 10 September 2015
Line 2:		Line 2:
	<StructureSection load='1y4e' size='340' side='right' caption='[[1y4e]], [[NMR_Ensembles_of_Models \| 100 NMR models]]' scene=''>		<StructureSection load='1y4e' size='340' side='right' caption='[[1y4e]], [[NMR_Ensembles_of_Models \| 100 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1y4e]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Homo_sapiens Homo sapiens~~]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Y4E OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1Y4E FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1y4e]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Y4E OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1Y4E FirstGlance]. <br>
	</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=HSL:HOMOSERINE+LACTONE'>HSL</scene></td></tr>		</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=HSL:HOMOSERINE+LACTONE'>HSL</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">SLC9A1, APNH1, NHE1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 ~~Homo sapiens~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">SLC9A1, APNH1, NHE1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1y4e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1y4e OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1y4e RCSB], [http://www.ebi.ac.uk/pdbsum/1y4e PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1y4e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1y4e OCA], [http://pdbe.org/1y4e PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1y4e RCSB], [http://www.ebi.ac.uk/pdbsum/1y4e PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 17:		Line 17:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1y4e" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Homo sapiens~~]]	+	[[Category: Human]]
	[[Category: Fliegel, L]]		[[Category: Fliegel, L]]
	[[Category: Li, X]]		[[Category: Li, X]]

OCA at 21:30, 24 December 2014

OCA — Wed, 24 Dec 2014 21:30:40 GMT

←Older revision		Revision as of 21:30, 24 December 2014
Line 7:		Line 7:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1y4e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1y4e OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1y4e RCSB], [http://www.ebi.ac.uk/pdbsum/1y4e PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1y4e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1y4e OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1y4e RCSB], [http://www.ebi.ac.uk/pdbsum/1y4e PDBsum]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/SL9A1_HUMAN SL9A1_HUMAN]] Involved in pH regulation to eliminate acids generated by active metabolism or to counter adverse environmental conditions. Major proton extruding system driven by the inward sodium ion chemical gradient. Plays an important role in signal transduction.<ref>PMID:8901634</ref> <ref>PMID:11350981</ref> <ref>PMID:15035633</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 20:		Line 22:
	</StructureSection>		</StructureSection>
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
-	[[Category: Fliegel, L.]]	+	[[Category: Fliegel, L]]
-	[[Category: Li, X.]]	+	[[Category: Li, X]]
-	[[Category: Lindhout, D A.]]	+	[[Category: Lindhout, D A]]
-	[[Category: Liu, Y.]]	+	[[Category: Liu, Y]]
-	[[Category: Rainey, J K.]]	+	[[Category: Rainey, J K]]
-	[[Category: Slepkov, E R.]]	+	[[Category: Slepkov, E R]]
-	[[Category: Sykes, B D.]]	+	[[Category: Sykes, B D]]
	[[Category: Membrane protein]]		[[Category: Membrane protein]]
	[[Category: Nhe1 isoform]]		[[Category: Nhe1 isoform]]
	[[Category: Transmembrane]]		[[Category: Transmembrane]]

OCA at 08:12, 8 October 2014

OCA — Wed, 08 Oct 2014 08:12:39 GMT

←Older revision		Revision as of 08:12, 8 October 2014
Line 1:		Line 1:
-	[[~~Image:~~1y4e.~~png~~\|~~left~~\|~~200px~~]]	+	==NMR structure of transmembrane segment IV of the NHE1 isoform of the Na+/H+ exchanger==
		+	<StructureSection load='1y4e' size='340' side='right' caption='[[1y4e]], [[NMR_Ensembles_of_Models \| 100 NMR models]]' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1y4e]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Y4E OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1Y4E FirstGlance]. <br>
		+	</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=HSL:HOMOSERINE+LACTONE'>HSL</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">SLC9A1, APNH1, NHE1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1y4e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1y4e OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1y4e RCSB], [http://www.ebi.ac.uk/pdbsum/1y4e PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The Na(+)/H(+) exchanger isoform 1 is a ubiquitously expressed integral membrane protein that regulates intracellular pH in mammals. We characterized the structural and functional aspects of the critical transmembrane (TM) segment IV. Each residue was mutated to cysteine in cysteine-less NHE1. TM IV was exquisitely sensitive to mutation with 10 of 23 mutations causing greatly reduced expression and/or activity. The Phe(161) --> Cys mutant was inhibited by treatment with the water-soluble sulfhydryl-reactive compounds [2-(trimethylammonium)ethyl]methanethiosulfonate and [2-sulfonatoethyl]methanethiosulfonate, suggesting it is a pore-lining residue. The structure of purified TM IV peptide was determined using high resolution NMR in a CD(3)OH:CDCl(3):H(2)O mixture and in Me(2)SO. In CD(3)OH: CDCl(3):H(2)O, TM IV was structured but not as a canonical alpha-helix. Residues Asp(159)-Leu(162) were a series of beta-turns; residues Leu(165)-Pro(168) showed an extended structure, and residues Ile(169)-Phe(176) were helical in character. These three structured regions rotated quite freely with respect to the others. In Me(2)SO, the structure was much less defined. Our results demonstrate that TM IV is an unusually structured transmembrane segment that is exquisitely sensitive to mutagenesis and that Phe(161) is a pore-lining residue.

-	~~{{STRUCTURE_1y4e\| PDB=1y4e \| SCENE= }}~~	+	Structural and functional characterization of transmembrane segment IV of the NHE1 isoform of the Na+/H+ exchanger.,Slepkov ER, Rainey JK, Li X, Liu Y, Cheng FJ, Lindhout DA, Sykes BD, Fliegel L J Biol Chem. 2005 May 6;280(18):17863-72. Epub 2005 Jan 26. PMID:15677483<ref>PMID:15677483</ref>

-	~~===NMR structure of transmembrane segment IV~~ of the ~~NHE1 isoform~~ of ~~the Na+~~/~~H+ exchanger===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_15677483}}~~	+	== References ==
-		+	<references/>
-	==~~About this Structure~~==	+	__TOC__
-	~~[[1y4e]] is a 1 chain structure with sequence from [http:~~//~~en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Y4E OCA].~~	+	</StructureSection>
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
	[[Category: Fliegel, L.]]		[[Category: Fliegel, L.]]

OCA: Protected "1y4e" [edit=sysop:move=sysop]

OCA — Sat, 05 Jan 2013 13:08:23 GMT

Protected "1y4e" [edit=sysop:move=sysop]

←Older revision

Revision as of 13:08, 5 January 2013

OCA at 13:08, 5 January 2013

OCA — Sat, 05 Jan 2013 13:08:18 GMT

←Older revision		Revision as of 13:08, 5 January 2013
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-	{{Seed}}
	[[Image:1y4e.png\|left\|200px]]		[[Image:1y4e.png\|left\|200px]]

-	<!--
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	{{STRUCTURE_1y4e\| PDB=1y4e \| SCENE= }}		{{STRUCTURE_1y4e\| PDB=1y4e \| SCENE= }}

	===NMR structure of transmembrane segment IV of the NHE1 isoform of the Na+/H+ exchanger===		===NMR structure of transmembrane segment IV of the NHE1 isoform of the Na+/H+ exchanger===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_15677483}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 15677483 is the PubMed ID number.
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	{{ABSTRACT_PUBMED_15677483}}		{{ABSTRACT_PUBMED_15677483}}

	==About this Structure==		==About this Structure==
-	~~1Y4E is a~~ [[~~Single protein~~]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Y4E OCA].	+	[[1y4e]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Y4E OCA].
-		+
-	~~==Reference==~~	+
-	Structural and functional characterization of transmembrane segment IV of the NHE1 isoform of the Na+/H+ exchanger., Slepkov ER, Rainey JK, Li X, Liu Y, Cheng FJ, Lindhout DA, Sykes BD, Fliegel L, J Biol Chem. 2005 May 6;280(18):17863-72. Epub 2005 Jan 26. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15677483 15677483]	+
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
-	[[Category: Single protein]]
	[[Category: Fliegel, L.]]		[[Category: Fliegel, L.]]
	[[Category: Li, X.]]		[[Category: Li, X.]]
Line 33:		Line 17:
	[[Category: Slepkov, E R.]]		[[Category: Slepkov, E R.]]
	[[Category: Sykes, B D.]]		[[Category: Sykes, B D.]]
		+	[[Category: Membrane protein]]
	[[Category: Nhe1 isoform]]		[[Category: Nhe1 isoform]]
	[[Category: Transmembrane]]		[[Category: Transmembrane]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 00:09:13 2008''

OCA at 21:09, 27 July 2008

OCA — Sun, 27 Jul 2008 21:09:18 GMT

←Older revision		Revision as of 21:09, 27 July 2008
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-	[[Image:1y4e.~~gif~~\|left\|200px]]	+	{{Seed}}
		+	[[Image:1y4e.png\|left\|200px]]

	<!--		<!--
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	{{STRUCTURE_1y4e\| PDB=1y4e \| SCENE= }}		{{STRUCTURE_1y4e\| PDB=1y4e \| SCENE= }}

-	~~'''~~NMR structure of transmembrane segment IV of the NHE1 isoform of the Na+/H+ exchanger~~'''~~	+	===NMR structure of transmembrane segment IV of the NHE1 isoform of the Na+/H+ exchanger===


-	~~==Overview==~~	+	<!--
-	The ~~Na(+)/H(+) exchanger isoform 1 is a ubiquitously expressed integral membrane protein that regulates intracellular pH in mammals. We characterized~~ the ~~structural and functional aspects of the critical transmembrane (TM) segment IV. Each residue was mutated~~ to cysteine in cysteine-less NHE1. TM IV was exquisitely sensitive to mutation with 10 of 23 mutations causing greatly reduced expression and/or activity. The Phe(161) --> Cys mutant was inhibited by treatment with the ~~water-soluble sulfhydryl-reactive compounds [2-~~(~~trimethylammonium)ethyl]methanethiosulfonate and [2-sulfonatoethyl]methanethiosulfonate, suggesting~~ it ~~is a pore-lining residue. The structure of purified TM IV peptide was determined using high resolution NMR in a CD(3)OH~~:~~CDCl(3):H(2)O mixture and in Me(2)SO~~. ~~In CD(3)OH: CDCl(3):H(2)O, TM IV was structured but not as a canonical alpha-helix~~. ~~Residues Asp(159~~)~~-Leu(162) were a series of beta-turns; residues Leu(165)-Pro(168) showed an extended structure~~, ~~and residues Ile(169)-Phe(176) were helical in character. These three structured regions rotated quite freely with respect to~~ the ~~others~~. In Me(2)SO, the structure was much less defined. Our results demonstrate that TM IV is an unusually structured transmembrane segment that is exquisitely sensitive to mutagenesis and that Phe(161) is a pore-~~lining residue.~~	+	The line below this paragraph, {{ABSTRACT_PUBMED_15677483}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 15677483 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_15677483}}

	==About this Structure==		==About this Structure==
-	1Y4E is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full ~~crystallographic~~ information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Y4E OCA].	+	1Y4E is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Y4E OCA].

	==Reference==		==Reference==
Line 31:		Line 35:
	[[Category: Nhe1 isoform]]		[[Category: Nhe1 isoform]]
	[[Category: Transmembrane]]		[[Category: Transmembrane]]
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Sat May 3 15~~:51:49 2008''	+
		+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 00:09:13 2008''