1y9g - Revision history

OCA at 08:01, 15 May 2024

OCA — Wed, 15 May 2024 08:01:34 GMT

←Older revision		Revision as of 08:01, 15 May 2024
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1y9g]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Aspergillus_awamori Aspergillus awamori]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Y9G OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1Y9G FirstGlance]. <br>		<table><tr><td colspan='2'>[[1y9g]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Aspergillus_awamori Aspergillus awamori]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Y9G OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1Y9G FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=FRU:FRUCTOSE'>FRU</scene>~~, ~~<scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.87Å</td></tr>
-	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"><~~div style~~='~~overflow~~: ~~auto; max-height: 3em;~~'>~~[[1y4w\|1y4w]]~~</~~div~~><~~/td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FRU:FRUCTOSE'>FRU</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
-	~~<tr id~~='~~activity'><td class~~=~~"sblockLbl"><b>Activity~~:~~</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Fructan_beta~~-~~fructosidase Fructan beta~~-~~fructosidase], with EC number [https://www.brenda~~-~~enzymes.info/php/result_flat.php4?ecno=3.2.1.80 3.2.1.80]~~ </~~span~~></td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1y9g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1y9g OCA], [https://pdbe.org/1y9g PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1y9g RCSB], [https://www.ebi.ac.uk/pdbsum/1y9g PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1y9g ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1y9g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1y9g OCA], [https://pdbe.org/1y9g PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1y9g RCSB], [https://www.ebi.ac.uk/pdbsum/1y9g PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1y9g ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/INUE_ASPAW INUE_ASPAW] Exo-inulinase involved in utilization of the plant storage polymer inulin, consisting of fructooligosaccharides with a degree of polymerization (DP) value from 2 to 60. Splits off terminal fructose units successively from the non-reducing end of the inulin molecule, and also hydrolyzes levan, stachyose and raffinose.<ref>PMID:11829749</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	</StructureSection>		</StructureSection>
	[[Category: Aspergillus awamori]]		[[Category: Aspergillus awamori]]
-	[[Category: Fructan beta-fructosidase]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Eneyskaya~~, E V~~]]	+	[[Category: Eneyskaya EV]]
-	[[Category: Golubev~~, A M~~]]	+	[[Category: Golubev AM]]
-	[[Category: Korneeva~~, O S~~]]	+	[[Category: Korneeva OS]]
-	[[Category: Kulminskaya~~, A A~~]]	+	[[Category: Kulminskaya AA]]
-	[[Category: Nagem~~, R A.P~~]]	+	[[Category: Nagem RAP]]
-	[[Category: Neustroev~~, K N~~]]	+	[[Category: Neustroev KN]]
-	[[Category: Polikarpov, I]]	+	[[Category: Polikarpov I]]
-	[[Category: Rojas~~, A L]]~~	+	[[Category: Rojas AL]]
-	~~[[Category: Complex with fructose]]~~	+
-	~~[[Category: Crystallographic structure]]~~	+
-	~~[[Category: Exo-inulinase]]~~	+
-	~~[[Category: Glycoside hydrolase family 32]]~~	+
-	~~[[Category: Hydrolase~~]]	+

OCA at 06:33, 6 October 2021

OCA — Wed, 06 Oct 2021 06:33:01 GMT

←Older revision		Revision as of 06:33, 6 October 2021
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	<StructureSection load='1y9g' size='340' side='right'caption='[[1y9g]], [[Resolution\|resolution]] 1.87Å' scene=''>		<StructureSection load='1y9g' size='340' side='right'caption='[[1y9g]], [[Resolution\|resolution]] 1.87Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1y9g]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Aspergillus_awamori Aspergillus awamori]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Y9G OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1Y9G FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1y9g]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Aspergillus_awamori Aspergillus awamori]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Y9G OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1Y9G FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FRU:FRUCTOSE'>FRU</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FRU:FRUCTOSE'>FRU</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1y4w\|1y4w]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1y4w\|1y4w]]</div></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Fructan_beta-fructosidase Fructan beta-fructosidase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.80 3.2.1.80] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Fructan_beta-fructosidase Fructan beta-fructosidase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.80 3.2.1.80] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1y9g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1y9g OCA], [~~http~~://pdbe.org/1y9g PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1y9g RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1y9g PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1y9g ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1y9g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1y9g OCA], [https://pdbe.org/1y9g PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1y9g RCSB], [https://www.ebi.ac.uk/pdbsum/1y9g PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1y9g ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==

OCA at 11:16, 4 December 2019

OCA — Wed, 04 Dec 2019 11:16:29 GMT

←Older revision		Revision as of 11:16, 4 December 2019
Line 1:		Line 1:

	==Crystal structure of exo-inulinase from Aspergillus awamori complexed with fructose==		==Crystal structure of exo-inulinase from Aspergillus awamori complexed with fructose==
-	<StructureSection load='1y9g' size='340' side='right' caption='[[1y9g]], [[Resolution\|resolution]] 1.87Å' scene=''>	+	<StructureSection load='1y9g' size='340' side='right'caption='[[1y9g]], [[Resolution\|resolution]] 1.87Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1y9g]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Aspergillus_awamori Aspergillus awamori]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Y9G OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1Y9G FirstGlance]. <br>		<table><tr><td colspan='2'>[[1y9g]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Aspergillus_awamori Aspergillus awamori]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Y9G OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1Y9G FirstGlance]. <br>
Line 34:		Line 34:
	[[Category: Aspergillus awamori]]		[[Category: Aspergillus awamori]]
	[[Category: Fructan beta-fructosidase]]		[[Category: Fructan beta-fructosidase]]
		+	[[Category: Large Structures]]
	[[Category: Eneyskaya, E V]]		[[Category: Eneyskaya, E V]]
	[[Category: Golubev, A M]]		[[Category: Golubev, A M]]

OCA at 06:31, 20 December 2017

OCA — Wed, 20 Dec 2017 06:31:04 GMT

←Older revision		Revision as of 06:31, 20 December 2017
Line 3:		Line 3:
	<StructureSection load='1y9g' size='340' side='right' caption='[[1y9g]], [[Resolution\|resolution]] 1.87Å' scene=''>		<StructureSection load='1y9g' size='340' side='right' caption='[[1y9g]], [[Resolution\|resolution]] 1.87Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1y9g]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Aspaw Aspaw~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Y9G OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1Y9G FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1y9g]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Aspergillus_awamori Aspergillus awamori]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Y9G OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1Y9G FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FRU:FRUCTOSE'>FRU</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FRU:FRUCTOSE'>FRU</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1y4w\|1y4w]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1y4w\|1y4w]]</td></tr>
Line 13:		Line 13:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/y9/1y9g_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/y9/1y9g_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
Line 32:		Line 32:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Aspaw~~]]	+	[[Category: Aspergillus awamori]]
	[[Category: Fructan beta-fructosidase]]		[[Category: Fructan beta-fructosidase]]
	[[Category: Eneyskaya, E V]]		[[Category: Eneyskaya, E V]]
Line 42:		Line 42:
	[[Category: Polikarpov, I]]		[[Category: Polikarpov, I]]
	[[Category: Rojas, A L]]		[[Category: Rojas, A L]]
-	[[Category: Aspergillus awamori]]
	[[Category: Complex with fructose]]		[[Category: Complex with fructose]]
	[[Category: Crystallographic structure]]		[[Category: Crystallographic structure]]

OCA at 08:16, 12 October 2017

OCA — Thu, 12 Oct 2017 08:16:35 GMT

←Older revision		Revision as of 08:16, 12 October 2017
Line 1:		Line 1:
		+
	==Crystal structure of exo-inulinase from Aspergillus awamori complexed with fructose==		==Crystal structure of exo-inulinase from Aspergillus awamori complexed with fructose==
	<StructureSection load='1y9g' size='340' side='right' caption='[[1y9g]], [[Resolution\|resolution]] 1.87Å' scene=''>		<StructureSection load='1y9g' size='340' side='right' caption='[[1y9g]], [[Resolution\|resolution]] 1.87Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1y9g]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Aspergillus_awamori Aspergillus awamori~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Y9G OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1Y9G FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1y9g]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Aspaw Aspaw]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Y9G OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1Y9G FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FRU:FRUCTOSE'>FRU</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FRU:FRUCTOSE'>FRU</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1y4w\|1y4w]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1y4w\|1y4w]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Fructan_beta-fructosidase Fructan beta-fructosidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.80 3.2.1.80] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Fructan_beta-fructosidase Fructan beta-fructosidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.80 3.2.1.80] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1y9g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1y9g OCA], [http://pdbe.org/1y9g PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1y9g RCSB], [http://www.ebi.ac.uk/pdbsum/1y9g PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1y9g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1y9g OCA], [http://pdbe.org/1y9g PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1y9g RCSB], [http://www.ebi.ac.uk/pdbsum/1y9g PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1y9g ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 16:		Line 17:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1y9g ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 31:		Line 32:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Aspergillus awamori~~]]	+	[[Category: Aspaw]]
	[[Category: Fructan beta-fructosidase]]		[[Category: Fructan beta-fructosidase]]
	[[Category: Eneyskaya, E V]]		[[Category: Eneyskaya, E V]]
Line 41:		Line 42:
	[[Category: Polikarpov, I]]		[[Category: Polikarpov, I]]
	[[Category: Rojas, A L]]		[[Category: Rojas, A L]]
		+	[[Category: Aspergillus awamori]]
	[[Category: Complex with fructose]]		[[Category: Complex with fructose]]
	[[Category: Crystallographic structure]]		[[Category: Crystallographic structure]]

OCA at 07:33, 11 September 2015

OCA — Fri, 11 Sep 2015 07:33:38 GMT

←Older revision		Revision as of 07:33, 11 September 2015
Line 6:		Line 6:
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1y4w\|1y4w]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1y4w\|1y4w]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Fructan_beta-fructosidase Fructan beta-fructosidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.80 3.2.1.80] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Fructan_beta-fructosidase Fructan beta-fructosidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.80 3.2.1.80] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1y9g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1y9g OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1y9g RCSB], [http://www.ebi.ac.uk/pdbsum/1y9g PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1y9g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1y9g OCA], [http://pdbe.org/1y9g PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1y9g RCSB], [http://www.ebi.ac.uk/pdbsum/1y9g PDBsum]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 26:		Line 26:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1y9g" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 09:02, 8 January 2015

OCA — Thu, 08 Jan 2015 09:02:50 GMT

←Older revision		Revision as of 09:02, 8 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1y9g]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Aspergillus_awamori Aspergillus awamori]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Y9G OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1Y9G FirstGlance]. <br>		<table><tr><td colspan='2'>[[1y9g]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Aspergillus_awamori Aspergillus awamori]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Y9G OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1Y9G FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FRU:FRUCTOSE'>FRU</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FRU:FRUCTOSE'>FRU</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1y4w\|1y4w]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1y4w\|1y4w]]</td></tr>
-	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Fructan_beta-fructosidase Fructan beta-fructosidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.80 3.2.1.80] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Fructan_beta-fructosidase Fructan beta-fructosidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.80 3.2.1.80] </span></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1y9g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1y9g OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1y9g RCSB], [http://www.ebi.ac.uk/pdbsum/1y9g PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1y9g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1y9g OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1y9g RCSB], [http://www.ebi.ac.uk/pdbsum/1y9g PDBsum]</span></td></tr>
-	<table>	+	</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 32:		Line 32:
	[[Category: Aspergillus awamori]]		[[Category: Aspergillus awamori]]
	[[Category: Fructan beta-fructosidase]]		[[Category: Fructan beta-fructosidase]]
-	[[Category: Eneyskaya, E V.]]	+	[[Category: Eneyskaya, E V]]
-	[[Category: Golubev, A M.]]	+	[[Category: Golubev, A M]]
-	[[Category: Korneeva, O S.]]	+	[[Category: Korneeva, O S]]
-	[[Category: Kulminskaya, A A.]]	+	[[Category: Kulminskaya, A A]]
-	[[Category: Nagem, R A.P.]]	+	[[Category: Nagem, R A.P]]
-	[[Category: Neustroev, K N.]]	+	[[Category: Neustroev, K N]]
-	[[Category: Polikarpov, I.]]	+	[[Category: Polikarpov, I]]
-	[[Category: Rojas, A L~~.]]~~	+	[[Category: Rojas, A L]]
-	~~[[Category: Aspergillus awamori~~]]	+
	[[Category: Complex with fructose]]		[[Category: Complex with fructose]]
	[[Category: Crystallographic structure]]		[[Category: Crystallographic structure]]

OCA at 20:22, 29 September 2014

OCA — Mon, 29 Sep 2014 20:22:26 GMT

←Older revision		Revision as of 20:22, 29 September 2014
Line 1:		Line 1:
-	[[~~Image:~~1y9g.~~png~~\|~~left~~\|200px]]	+	==Crystal structure of exo-inulinase from Aspergillus awamori complexed with fructose==
		+	<StructureSection load='1y9g' size='340' side='right' caption='[[1y9g]], [[Resolution\|resolution]] 1.87Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1y9g]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Aspergillus_awamori Aspergillus awamori]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Y9G OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1Y9G FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FRU:FRUCTOSE'>FRU</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1y4w\|1y4w]]</td></tr>
		+	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Fructan_beta-fructosidase Fructan beta-fructosidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.80 3.2.1.80] </span></td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1y9g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1y9g OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1y9g RCSB], [http://www.ebi.ac.uk/pdbsum/1y9g PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/y9/1y9g_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Exo-inulinases hydrolyze terminal, non-reducing 2,1-linked and 2,6-linked beta-d-fructofuranose residues in inulin, levan and sucrose releasing beta-d-fructose. We present the X-ray structure at 1.55A resolution of exo-inulinase from Aspergillus awamori, a member of glycoside hydrolase family 32, solved by single isomorphous replacement with the anomalous scattering method using the heavy-atom sites derived from a quick cryo-soaking technique. The tertiary structure of this enzyme folds into two domains: the N-terminal catalytic domain of an unusual five-bladed beta-propeller fold and the C-terminal domain folded into a beta-sandwich-like structure. Its structural architecture is very similar to that of another member of glycoside hydrolase family 32, invertase (beta-fructosidase) from Thermotoga maritima, determined recently by X-ray crystallography The exo-inulinase is a glycoprotein containing five N-linked oligosaccharides. Two crystal forms obtained under similar crystallization conditions differ by the degree of protein glycosylation. The X-ray structure of the enzyme:fructose complex, at a resolution of 1.87A, reveals two catalytically important residues: Asp41 and Glu241, a nucleophile and a catalytic acid/base, respectively. The distance between the side-chains of these residues is consistent with a double displacement mechanism of reaction. Asp189, which is part of the Arg-Asp-Pro motif, provides hydrogen bonds important for substrate recognition.

-	~~{{STRUCTURE_1y9g\| PDB=1y9g \| SCENE= }}~~	+	Crystal structure of exo-inulinase from Aspergillus awamori: the enzyme fold and structural determinants of substrate recognition.,Nagem RA, Rojas AL, Golubev AM, Korneeva OS, Eneyskaya EV, Kulminskaya AA, Neustroev KN, Polikarpov I J Mol Biol. 2004 Nov 19;344(2):471-80. PMID:15522299<ref>PMID:15522299</ref>

-	~~===Crystal structure~~ of ~~exo-inulinase from Aspergillus awamori complexed with fructose===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_15522299}}~~	+	== References ==
-		+	<references/>
-	~~==About this Structure==~~	+	__TOC__
-	~~[[1y9g]] is a 1 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Aspergillus_awamori Aspergillus awamori]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=1Y9G OCA].~~	+	</StructureSection>
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:015522299<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Aspergillus awamori]]		[[Category: Aspergillus awamori]]
	[[Category: Fructan beta-fructosidase]]		[[Category: Fructan beta-fructosidase]]

OCA at 12:22, 23 January 2013

OCA — Wed, 23 Jan 2013 12:22:30 GMT

←Older revision		Revision as of 12:22, 23 January 2013
Line 1:		Line 1:
	[[Image:1y9g.png\|left\|200px]]		[[Image:1y9g.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_1y9g", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_1y9g\| PDB=1y9g \| SCENE= }}		{{STRUCTURE_1y9g\| PDB=1y9g \| SCENE= }}

	===Crystal structure of exo-inulinase from Aspergillus awamori complexed with fructose===		===Crystal structure of exo-inulinase from Aspergillus awamori complexed with fructose===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_15522299}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 15522299 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_15522299}}		{{ABSTRACT_PUBMED_15522299}}

Line 22:		Line 11:

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~15522299~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:015522299</ref><references group="xtra"/>
	[[Category: Aspergillus awamori]]		[[Category: Aspergillus awamori]]
	[[Category: Fructan beta-fructosidase]]		[[Category: Fructan beta-fructosidase]]
Line 33:		Line 22:
	[[Category: Polikarpov, I.]]		[[Category: Polikarpov, I.]]
	[[Category: Rojas, A L.]]		[[Category: Rojas, A L.]]
		+	[[Category: Aspergillus awamori]]
		+	[[Category: Complex with fructose]]
		+	[[Category: Crystallographic structure]]
		+	[[Category: Exo-inulinase]]
		+	[[Category: Glycoside hydrolase family 32]]
		+	[[Category: Hydrolase]]

OCA: Protected "1y9g" [edit=sysop:move=sysop]

OCA — Wed, 16 Mar 2011 07:30:00 GMT

Protected "1y9g" [edit=sysop:move=sysop]

←Older revision

Revision as of 07:30, 16 March 2011