1ygm - Revision history

OCA at 09:30, 6 December 2023

OCA — Wed, 06 Dec 2023 09:30:47 GMT

←Older revision		Revision as of 09:30, 6 December 2023
Line 1:		Line 1:

	==NMR structure of Mistic==		==NMR structure of Mistic==
-	<StructureSection load='1ygm' size='340' side='right'caption='[[1ygm~~]], [[NMR_Ensembles_of_Models \| 10 NMR models~~]]' scene=''>	+	<StructureSection load='1ygm' size='340' side='right'caption='[[1ygm]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1ygm]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Bacsu Bacsu~~]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YGM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1YGM FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1ygm]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_subtilis_subsp._subtilis_str._168 Bacillus subtilis subsp. subtilis str. 168]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YGM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1YGM FirstGlance]. <br>
-	</td></tr><tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat"~~>YugO-a ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&~~id=~~224308 BACSU])~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ygm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ygm OCA], [https://pdbe.org/1ygm PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ygm RCSB], [https://www.ebi.ac.uk/pdbsum/1ygm PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ygm ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ygm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ygm OCA], [https://pdbe.org/1ygm PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ygm RCSB], [https://www.ebi.ac.uk/pdbsum/1ygm PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ygm ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/MSTX_BACSU MSTX_BACSU]] Chaperone that facilitates the production and integration of integral membrane proteins into the bacterial lipid bilayer.<ref>PMID:15731457</ref>	+	[https://www.uniprot.org/uniprot/MSTX_BACSU MSTX_BACSU] Chaperone that facilitates the production and integration of integral membrane proteins into the bacterial lipid bilayer.<ref>PMID:15731457</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 22:		Line 22:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Bacsu~~]]	+	[[Category: Bacillus subtilis subsp. subtilis str. 168]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Castronovo, S]]	+	[[Category: Castronovo S]]
-	[[Category: Choe, S]]	+	[[Category: Choe S]]
-	[[Category: Greenwald, J]]	+	[[Category: Greenwald J]]
-	[[Category: Riek, R]]	+	[[Category: Riek R]]
-	[[Category: Roosild~~, T P~~]]	+	[[Category: Roosild TP]]
-	[[Category: Vega, M]]	+	[[Category: Vega M]]
-	~~[[Category: Alpha-helical bundle]]~~	+
-	~~[[Category: Integral membrane protein]]~~	+
-	~~[[Category: Membrane protein~~]]	+

OCA at 07:12, 2 March 2022

OCA — Wed, 02 Mar 2022 07:12:07 GMT

←Older revision		Revision as of 07:12, 2 March 2022
Line 3:		Line 3:
	<StructureSection load='1ygm' size='340' side='right'caption='[[1ygm]], [[NMR_Ensembles_of_Models \| 10 NMR models]]' scene=''>		<StructureSection load='1ygm' size='340' side='right'caption='[[1ygm]], [[NMR_Ensembles_of_Models \| 10 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1ygm]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Bacsu Bacsu]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YGM OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=1YGM FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1ygm]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacsu Bacsu]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YGM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1YGM FirstGlance]. <br>
-	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">YugO-a ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=224308 BACSU])</td></tr>	+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">YugO-a ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=224308 BACSU])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=1ygm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ygm OCA], [~~http~~://pdbe.org/1ygm PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1ygm RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1ygm PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1ygm ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ygm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ygm OCA], [https://pdbe.org/1ygm PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ygm RCSB], [https://www.ebi.ac.uk/pdbsum/1ygm PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ygm ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/MSTX_BACSU MSTX_BACSU]] Chaperone that facilitates the production and integration of integral membrane proteins into the bacterial lipid bilayer.<ref>PMID:15731457</ref>	+	[[https://www.uniprot.org/uniprot/MSTX_BACSU MSTX_BACSU]] Chaperone that facilitates the production and integration of integral membrane proteins into the bacterial lipid bilayer.<ref>PMID:15731457</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==

OCA at 08:45, 20 January 2021

OCA — Wed, 20 Jan 2021 08:45:22 GMT

←Older revision		Revision as of 08:45, 20 January 2021
Line 1:		Line 1:

	==NMR structure of Mistic==		==NMR structure of Mistic==
-	<StructureSection load='1ygm' size='340' side='right' caption='[[1ygm]], [[NMR_Ensembles_of_Models \| 10 NMR models]]' scene=''>	+	<StructureSection load='1ygm' size='340' side='right'caption='[[1ygm]], [[NMR_Ensembles_of_Models \| 10 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1ygm]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacsu Bacsu]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YGM OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1YGM FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1ygm]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacsu Bacsu]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YGM OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=1YGM FirstGlance]. <br>
	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">YugO-a ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=224308 BACSU])</td></tr>		</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">YugO-a ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=224308 BACSU])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1ygm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ygm OCA], [http://pdbe.org/1ygm PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1ygm RCSB], [http://www.ebi.ac.uk/pdbsum/1ygm PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1ygm ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=1ygm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ygm OCA], [http://pdbe.org/1ygm PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1ygm RCSB], [http://www.ebi.ac.uk/pdbsum/1ygm PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1ygm ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 23:		Line 23:
	</StructureSection>		</StructureSection>
	[[Category: Bacsu]]		[[Category: Bacsu]]
		+	[[Category: Large Structures]]
	[[Category: Castronovo, S]]		[[Category: Castronovo, S]]
	[[Category: Choe, S]]		[[Category: Choe, S]]

OCA at 07:10, 25 April 2018

OCA — Wed, 25 Apr 2018 07:10:56 GMT

←Older revision		Revision as of 07:10, 25 April 2018
Line 1:		Line 1:
		+
	==NMR structure of Mistic==		==NMR structure of Mistic==
	<StructureSection load='1ygm' size='340' side='right' caption='[[1ygm]], [[NMR_Ensembles_of_Models \| 10 NMR models]]' scene=''>		<StructureSection load='1ygm' size='340' side='right' caption='[[1ygm]], [[NMR_Ensembles_of_Models \| 10 NMR models]]' scene=''>
Line 4:		Line 5:
	<table><tr><td colspan='2'>[[1ygm]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacsu Bacsu]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YGM OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1YGM FirstGlance]. <br>		<table><tr><td colspan='2'>[[1ygm]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacsu Bacsu]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YGM OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1YGM FirstGlance]. <br>
	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">YugO-a ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=224308 BACSU])</td></tr>		</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">YugO-a ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=224308 BACSU])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ygm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ygm OCA], [http://pdbe.org/1ygm PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1ygm RCSB], [http://www.ebi.ac.uk/pdbsum/1ygm PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ygm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ygm OCA], [http://pdbe.org/1ygm PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1ygm RCSB], [http://www.ebi.ac.uk/pdbsum/1ygm PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1ygm ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==

OCA at 03:02, 10 September 2015

OCA — Thu, 10 Sep 2015 03:02:01 GMT

←Older revision		Revision as of 03:02, 10 September 2015
Line 2:		Line 2:
	<StructureSection load='1ygm' size='340' side='right' caption='[[1ygm]], [[NMR_Ensembles_of_Models \| 10 NMR models]]' scene=''>		<StructureSection load='1ygm' size='340' side='right' caption='[[1ygm]], [[NMR_Ensembles_of_Models \| 10 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1ygm]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Bacillus_subtilis_subsp._subtilis_str._168 Bacillus subtilis subsp. subtilis str. 168~~]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YGM OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1YGM FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1ygm]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacsu Bacsu]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YGM OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1YGM FirstGlance]. <br>
-	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">YugO-a ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=224308 ~~Bacillus subtilis subsp. subtilis str. 168~~])</td></tr>	+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">YugO-a ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=224308 BACSU])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ygm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ygm OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1ygm RCSB], [http://www.ebi.ac.uk/pdbsum/1ygm PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ygm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ygm OCA], [http://pdbe.org/1ygm PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1ygm RCSB], [http://www.ebi.ac.uk/pdbsum/1ygm PDBsum]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/MSTX_BACSU MSTX_BACSU]] Chaperone that facilitates the production and integration of integral membrane proteins into the bacterial lipid bilayer.<ref>PMID:15731457</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 14:		Line 16:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1ygm" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Bacillus subtilis subsp. subtilis str. 168~~]]	+	[[Category: Bacsu]]
	[[Category: Castronovo, S]]		[[Category: Castronovo, S]]
	[[Category: Choe, S]]		[[Category: Choe, S]]

OCA at 17:20, 15 January 2015

OCA — Thu, 15 Jan 2015 17:20:33 GMT

←Older revision		Revision as of 17:20, 15 January 2015
Line 19:		Line 19:
	</StructureSection>		</StructureSection>
	[[Category: Bacillus subtilis subsp. subtilis str. 168]]		[[Category: Bacillus subtilis subsp. subtilis str. 168]]
-	[[Category: Castronovo, S.]]	+	[[Category: Castronovo, S]]
-	[[Category: Choe, S.]]	+	[[Category: Choe, S]]
-	[[Category: Greenwald, J.]]	+	[[Category: Greenwald, J]]
-	[[Category: Riek, R.]]	+	[[Category: Riek, R]]
-	[[Category: Roosild, T P.]]	+	[[Category: Roosild, T P]]
-	[[Category: Vega, M.]]	+	[[Category: Vega, M]]
	[[Category: Alpha-helical bundle]]		[[Category: Alpha-helical bundle]]
	[[Category: Integral membrane protein]]		[[Category: Integral membrane protein]]
	[[Category: Membrane protein]]		[[Category: Membrane protein]]

OCA at 08:34, 8 October 2014

OCA — Wed, 08 Oct 2014 08:34:49 GMT

←Older revision		Revision as of 08:34, 8 October 2014
Line 1:		Line 1:
-	[[~~Image:~~1ygm.~~png~~\|~~left\|200px~~]]	+	==NMR structure of Mistic==
		+	<StructureSection load='1ygm' size='340' side='right' caption='[[1ygm]], [[NMR_Ensembles_of_Models \| 10 NMR models]]' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1ygm]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis_subsp._subtilis_str._168 Bacillus subtilis subsp. subtilis str. 168]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YGM OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1YGM FirstGlance]. <br>
		+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">YugO-a ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=224308 Bacillus subtilis subsp. subtilis str. 168])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ygm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ygm OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1ygm RCSB], [http://www.ebi.ac.uk/pdbsum/1ygm PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Although structure determination of soluble proteins has become routine, our understanding of membrane proteins has been limited by experimental bottlenecks in obtaining both sufficient yields of protein and ordered crystals. Mistic is an unusual Bacillus subtilis integral membrane protein that folds autonomously into the membrane, bypassing the cellular translocon machinery. Using paramagnetic probes, we determined by nuclear magnetic resonance (NMR) spectroscopy that the protein forms a helical bundle with a surprisingly polar lipid-facing surface. Additional experiments suggest that Mistic can be used for high-level production of other membrane proteins in their native conformations, including many eukaryotic proteins that have previously been intractable to bacterial expression.

-	~~{{STRUCTURE_1ygm\| PDB=1ygm \| SCENE= }}~~	+	NMR structure of Mistic, a membrane-integrating protein for membrane protein expression.,Roosild TP, Greenwald J, Vega M, Castronovo S, Riek R, Choe S Science. 2005 Feb 25;307(5713):1317-21. PMID:15731457<ref>PMID:15731457</ref>

-	~~===NMR structure~~ of ~~Mistic===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_15731457}}~~	+	== References ==
-		+	<references/>
-	==~~About this Structure~~==	+	__TOC__
-	~~[[1ygm]] is a 1 chain structure with sequence from [http:~~//en.wikipedia.org/wiki/Bacillus_subtilis_subsp._subtilis_str._168 Bacillus subtilis subsp. subtilis str. 168]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YGM OCA].	+	</StructureSection>
	[[Category: Bacillus subtilis subsp. subtilis str. 168]]		[[Category: Bacillus subtilis subsp. subtilis str. 168]]
	[[Category: Castronovo, S.]]		[[Category: Castronovo, S.]]

OCA: Protected "1ygm" [edit=sysop:move=sysop]

OCA — Sat, 05 Jan 2013 17:55:42 GMT

Protected "1ygm" [edit=sysop:move=sysop]

←Older revision

Revision as of 17:55, 5 January 2013

OCA at 17:55, 5 January 2013

OCA — Sat, 05 Jan 2013 17:55:39 GMT

←Older revision		Revision as of 17:55, 5 January 2013
Line 1:		Line 1:
-	{{Seed}}
	[[Image:1ygm.png\|left\|200px]]		[[Image:1ygm.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_1ygm", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
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	{{STRUCTURE_1ygm\| PDB=1ygm \| SCENE= }}		{{STRUCTURE_1ygm\| PDB=1ygm \| SCENE= }}

	===NMR structure of Mistic===		===NMR structure of Mistic===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_15731457}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 15731457 is the PubMed ID number.
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	{{ABSTRACT_PUBMED_15731457}}		{{ABSTRACT_PUBMED_15731457}}

	==About this Structure==		==About this Structure==
-	~~1YGM is a~~ [[~~Single protein~~]] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis_subsp._subtilis_str._168 Bacillus subtilis subsp. subtilis str. 168]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YGM OCA].	+	[[1ygm]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis_subsp._subtilis_str._168 Bacillus subtilis subsp. subtilis str. 168]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YGM OCA].
-		+
-	~~==Reference==~~	+
-	NMR structure of Mistic, a membrane-integrating protein for membrane protein expression., Roosild TP, Greenwald J, Vega M, Castronovo S, Riek R, Choe S, Science. 2005 Feb 25;307(5713):1317-21. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15731457 15731457]	+
	[[Category: Bacillus subtilis subsp. subtilis str. 168]]		[[Category: Bacillus subtilis subsp. subtilis str. 168]]
-	[[Category: Single protein]]
	[[Category: Castronovo, S.]]		[[Category: Castronovo, S.]]
	[[Category: Choe, S.]]		[[Category: Choe, S.]]
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	[[Category: Alpha-helical bundle]]		[[Category: Alpha-helical bundle]]
	[[Category: Integral membrane protein]]		[[Category: Integral membrane protein]]
-		+	[[Category: Membrane protein]]
-	~~''Page seeded by~~ [~~http~~:~~//oca.weizmann.ac.il/oca OCA~~ ] ~~on Sun Jul 27 17:44:14 2008''~~	+

OCA at 14:44, 27 July 2008

OCA — Sun, 27 Jul 2008 14:44:18 GMT

←Older revision		Revision as of 14:44, 27 July 2008
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-	[[Image:1ygm.~~gif~~\|left\|200px]]	+	{{Seed}}
		+	[[Image:1ygm.png\|left\|200px]]

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	{{STRUCTURE_1ygm\| PDB=1ygm \| SCENE= }}		{{STRUCTURE_1ygm\| PDB=1ygm \| SCENE= }}

-	~~'''~~NMR structure of Mistic~~'''~~	+	===NMR structure of Mistic===


-	~~==Overview==~~	+	<!--
-	~~Although structure determination of soluble proteins has become routine~~, our understanding of membrane proteins has been limited by experimental bottlenecks in obtaining both sufficient yields of protein and ordered crystals. Mistic is an unusual Bacillus subtilis integral membrane protein that folds autonomously into the membrane, ~~bypassing~~ the ~~cellular translocon machinery. Using paramagnetic probes, we determined by nuclear magnetic resonance~~ (~~NMR~~) ~~spectroscopy that~~ the ~~protein forms a helical bundle with a surprisingly polar lipid-facing surface~~. ~~Additional experiments suggest that Mistic can be used for high~~-~~level production of other membrane proteins in their native conformations, including many eukaryotic proteins that have previously been intractable to bacterial expression.~~	+	The line below this paragraph, {{ABSTRACT_PUBMED_15731457}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 15731457 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_15731457}}

	==About this Structure==		==About this Structure==
-	1YGM is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis_subsp._subtilis_str._168 Bacillus subtilis subsp. subtilis str. 168]. Full ~~crystallographic~~ information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YGM OCA].	+	1YGM is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis_subsp._subtilis_str._168 Bacillus subtilis subsp. subtilis str. 168]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YGM OCA].

	==Reference==		==Reference==
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	[[Category: Alpha-helical bundle]]		[[Category: Alpha-helical bundle]]
	[[Category: Integral membrane protein]]		[[Category: Integral membrane protein]]
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Sat May 3 16:~~17:35 2008''	+
		+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 17:44:14 2008''