1yj7 - Revision history

OCA at 08:57, 14 February 2024

OCA — Wed, 14 Feb 2024 08:57:56 GMT

←Older revision		Revision as of 08:57, 14 February 2024
Line 3:		Line 3:
	<StructureSection load='1yj7' size='340' side='right'caption='[[1yj7]], [[Resolution\|resolution]] 1.80Å' scene=''>		<StructureSection load='1yj7' size='340' side='right'caption='[[1yj7]], [[Resolution\|resolution]] 1.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1yj7]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/~~"bacillus_coli"_migula_1895 "bacillus~~ coli~~" migula 1895~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YJ7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1YJ7 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1yj7]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YJ7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1YJ7 FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>~~, ~~<scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.8Å</td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat">~~EscJ ([https~~:/~~/www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode~~=~~Info&srchmode~~=~~5&id=562 "Bacillus coli" Migula 1895])~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1yj7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1yj7 OCA], [https://pdbe.org/1yj7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1yj7 RCSB], [https://www.ebi.ac.uk/pdbsum/1yj7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1yj7 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1yj7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1yj7 OCA], [https://pdbe.org/1yj7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1yj7 RCSB], [https://www.ebi.ac.uk/pdbsum/1yj7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1yj7 ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/Q8VQD3_ECOLX Q8VQD3_ECOLX]
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 18:		Line 20:
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1yj7 ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1yj7 ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	Type III secretion systems (TTSSs) are multi-protein macromolecular 'machines' that have a central function in the virulence of many Gram-negative pathogens by directly mediating the secretion and translocation of bacterial proteins (termed effectors) into the cytoplasm of eukaryotic cells. Most of the 20 unique structural components constituting this secretion apparatus are highly conserved among animal and plant pathogens and are also evolutionarily related to proteins in the flagellar-specific export system. Recent electron microscopy experiments have revealed the gross 'needle-shaped' morphology of the TTSS, yet a detailed understanding of the structural characteristics and organization of these protein components within the bacterial membranes is lacking. Here we report the 1.8-A crystal structure of EscJ from enteropathogenic Escherichia coli (EPEC), a member of the YscJ/PrgK family whose oligomerization represents one of the earliest events in TTSS assembly. Crystal packing analysis and molecular modelling indicate that EscJ could form a large 24-subunit 'ring' superstructure with extensive grooves, ridges and electrostatic features. Electron microscopy, labelling and mass spectrometry studies on the orthologous Salmonella typhimurium PrgK within the context of the assembled TTSS support the stoichiometry, membrane association and surface accessibility of the modelled ring. We propose that the YscJ/PrgK protein family functions as an essential molecular platform for TTSS assembly.
-
-	Structural characterization of the molecular platform for type III secretion system assembly.,Yip CK, Kimbrough TG, Felise HB, Vuckovic M, Thomas NA, Pfuetzner RA, Frey EA, Finlay BB, Miller SI, Strynadka NC Nature. 2005 Jun 2;435(7042):702-7. PMID:15931226<ref>PMID:15931226</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 1yj7" style="background-color:#fffaf0;"></div>
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Bacillus~~ coli ~~migula 1895~~]]	+	[[Category: Escherichia coli]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Felise~~, H B~~]]	+	[[Category: Felise HB]]
-	[[Category: Finlay~~, B B~~]]	+	[[Category: Finlay BB]]
-	[[Category: Frey~~, E A~~]]	+	[[Category: Frey EA]]
-	[[Category: Kimbrough~~, T G~~]]	+	[[Category: Kimbrough TG]]
-	[[Category: Miller~~, S I~~]]	+	[[Category: Miller SI]]
-	[[Category: Pfuetzner~~, R A~~]]	+	[[Category: Pfuetzner RA]]
-	[[Category: Strynadka~~, N C.J~~]]	+	[[Category: Strynadka NCJ]]
-	[[Category: Thomas~~, N A~~]]	+	[[Category: Thomas NA]]
-	[[Category: Vuckovic, M]]	+	[[Category: Vuckovic M]]
-	[[Category: Yip~~, C K]]~~	+	[[Category: Yip CK]]
-	~~[[Category: Extended linker]]~~	+
-	~~[[Category: Mixed alpha/beta]]~~	+
-	~~[[Category: Protein transport~~]]	+

OCA at 06:07, 28 April 2021

OCA — Wed, 28 Apr 2021 06:07:16 GMT

←Older revision		Revision as of 06:07, 28 April 2021
Line 3:		Line 3:
	<StructureSection load='1yj7' size='340' side='right'caption='[[1yj7]], [[Resolution\|resolution]] 1.80Å' scene=''>		<StructureSection load='1yj7' size='340' side='right'caption='[[1yj7]], [[Resolution\|resolution]] 1.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1yj7]] is a 4 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YJ7 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1YJ7 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1yj7]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YJ7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1YJ7 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">EscJ ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 "Bacillus coli" Migula 1895])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">EscJ ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 "Bacillus coli" Migula 1895])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1yj7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1yj7 OCA], [~~http~~://pdbe.org/1yj7 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1yj7 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1yj7 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1yj7 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1yj7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1yj7 OCA], [https://pdbe.org/1yj7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1yj7 RCSB], [https://www.ebi.ac.uk/pdbsum/1yj7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1yj7 ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==

OCA at 07:08, 24 July 2019

OCA — Wed, 24 Jul 2019 07:08:31 GMT

←Older revision		Revision as of 07:08, 24 July 2019
Line 1:		Line 1:

	==Crystal structure of enteropathogenic E.coli (EPEC) type III secretion system protein EscJ==		==Crystal structure of enteropathogenic E.coli (EPEC) type III secretion system protein EscJ==
-	<StructureSection load='1yj7' size='340' side='right' caption='[[1yj7]], [[Resolution\|resolution]] 1.80Å' scene=''>	+	<StructureSection load='1yj7' size='340' side='right'caption='[[1yj7]], [[Resolution\|resolution]] 1.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1yj7]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YJ7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1YJ7 FirstGlance]. <br>		<table><tr><td colspan='2'>[[1yj7]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YJ7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1YJ7 FirstGlance]. <br>
Line 32:		Line 32:
	</StructureSection>		</StructureSection>
	[[Category: Bacillus coli migula 1895]]		[[Category: Bacillus coli migula 1895]]
		+	[[Category: Large Structures]]
	[[Category: Felise, H B]]		[[Category: Felise, H B]]
	[[Category: Finlay, B B]]		[[Category: Finlay, B B]]

OCA at 07:03, 25 April 2018

OCA — Wed, 25 Apr 2018 07:03:52 GMT

←Older revision		Revision as of 07:03, 25 April 2018
Line 1:		Line 1:
		+
	==Crystal structure of enteropathogenic E.coli (EPEC) type III secretion system protein EscJ==		==Crystal structure of enteropathogenic E.coli (EPEC) type III secretion system protein EscJ==
	<StructureSection load='1yj7' size='340' side='right' caption='[[1yj7]], [[Resolution\|resolution]] 1.80Å' scene=''>		<StructureSection load='1yj7' size='340' side='right' caption='[[1yj7]], [[Resolution\|resolution]] 1.80Å' scene=''>
Line 5:		Line 6:
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">EscJ ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 "Bacillus coli" Migula 1895])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">EscJ ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 "Bacillus coli" Migula 1895])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1yj7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1yj7 OCA], [http://pdbe.org/1yj7 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1yj7 RCSB], [http://www.ebi.ac.uk/pdbsum/1yj7 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1yj7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1yj7 OCA], [http://pdbe.org/1yj7 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1yj7 RCSB], [http://www.ebi.ac.uk/pdbsum/1yj7 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1yj7 ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 11:		Line 12:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/yj/1yj7_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/yj/1yj7_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>

OCA at 14:06, 9 February 2016

OCA — Tue, 09 Feb 2016 14:06:09 GMT

←Older revision		Revision as of 14:06, 9 February 2016
Line 15:		Line 15:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1yj7 ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 22:57, 10 September 2015

OCA — Thu, 10 Sep 2015 22:57:39 GMT

←Older revision		Revision as of 22:57, 10 September 2015
Line 2:		Line 2:
	<StructureSection load='1yj7' size='340' side='right' caption='[[1yj7]], [[Resolution\|resolution]] 1.80Å' scene=''>		<StructureSection load='1yj7' size='340' side='right' caption='[[1yj7]], [[Resolution\|resolution]] 1.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1yj7]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Escherichia_coli Escherichia~~ coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YJ7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1YJ7 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1yj7]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YJ7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1YJ7 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">EscJ ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 ~~Escherichia~~ coli])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">EscJ ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 "Bacillus coli" Migula 1895])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1yj7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1yj7 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1yj7 RCSB], [http://www.ebi.ac.uk/pdbsum/1yj7 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1yj7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1yj7 OCA], [http://pdbe.org/1yj7 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1yj7 RCSB], [http://www.ebi.ac.uk/pdbsum/1yj7 PDBsum]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 25:		Line 25:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1yj7" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Escherichia~~ coli]]	+	[[Category: Bacillus coli migula 1895]]
	[[Category: Felise, H B]]		[[Category: Felise, H B]]
	[[Category: Finlay, B B]]		[[Category: Finlay, B B]]

OCA at 09:45, 8 January 2015

OCA — Thu, 08 Jan 2015 09:45:57 GMT

←Older revision		Revision as of 09:45, 8 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1yj7]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YJ7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1YJ7 FirstGlance]. <br>		<table><tr><td colspan='2'>[[1yj7]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YJ7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1YJ7 FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">EscJ ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">EscJ ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1yj7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1yj7 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1yj7 RCSB], [http://www.ebi.ac.uk/pdbsum/1yj7 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1yj7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1yj7 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1yj7 RCSB], [http://www.ebi.ac.uk/pdbsum/1yj7 PDBsum]</span></td></tr>
-	<table>	+	</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 30:		Line 30:
	</StructureSection>		</StructureSection>
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
-	[[Category: Felise, H B.]]	+	[[Category: Felise, H B]]
-	[[Category: Finlay, B B.]]	+	[[Category: Finlay, B B]]
-	[[Category: Frey, E A.]]	+	[[Category: Frey, E A]]
-	[[Category: Kimbrough, T G.]]	+	[[Category: Kimbrough, T G]]
-	[[Category: Miller, S I.]]	+	[[Category: Miller, S I]]
-	[[Category: Pfuetzner, R A.]]	+	[[Category: Pfuetzner, R A]]
-	[[Category: Strynadka, N C.J.]]	+	[[Category: Strynadka, N C.J]]
-	[[Category: Thomas, N A.]]	+	[[Category: Thomas, N A]]
-	[[Category: Vuckovic, M.]]	+	[[Category: Vuckovic, M]]
-	[[Category: Yip, C K.]]	+	[[Category: Yip, C K]]
	[[Category: Extended linker]]		[[Category: Extended linker]]
	[[Category: Mixed alpha/beta]]		[[Category: Mixed alpha/beta]]
	[[Category: Protein transport]]		[[Category: Protein transport]]

OCA at 18:46, 29 September 2014

OCA — Mon, 29 Sep 2014 18:46:26 GMT

←Older revision		Revision as of 18:46, 29 September 2014
Line 1:		Line 1:
-	[[~~Image:~~1yj7.~~png~~\|~~left~~\|200px]]	+	==Crystal structure of enteropathogenic E.coli (EPEC) type III secretion system protein EscJ==
		+	<StructureSection load='1yj7' size='340' side='right' caption='[[1yj7]], [[Resolution\|resolution]] 1.80Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1yj7]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YJ7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1YJ7 FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">EscJ ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1yj7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1yj7 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1yj7 RCSB], [http://www.ebi.ac.uk/pdbsum/1yj7 PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/yj/1yj7_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Type III secretion systems (TTSSs) are multi-protein macromolecular 'machines' that have a central function in the virulence of many Gram-negative pathogens by directly mediating the secretion and translocation of bacterial proteins (termed effectors) into the cytoplasm of eukaryotic cells. Most of the 20 unique structural components constituting this secretion apparatus are highly conserved among animal and plant pathogens and are also evolutionarily related to proteins in the flagellar-specific export system. Recent electron microscopy experiments have revealed the gross 'needle-shaped' morphology of the TTSS, yet a detailed understanding of the structural characteristics and organization of these protein components within the bacterial membranes is lacking. Here we report the 1.8-A crystal structure of EscJ from enteropathogenic Escherichia coli (EPEC), a member of the YscJ/PrgK family whose oligomerization represents one of the earliest events in TTSS assembly. Crystal packing analysis and molecular modelling indicate that EscJ could form a large 24-subunit 'ring' superstructure with extensive grooves, ridges and electrostatic features. Electron microscopy, labelling and mass spectrometry studies on the orthologous Salmonella typhimurium PrgK within the context of the assembled TTSS support the stoichiometry, membrane association and surface accessibility of the modelled ring. We propose that the YscJ/PrgK protein family functions as an essential molecular platform for TTSS assembly.

-	~~{{STRUCTURE_1yj7\| PDB=1yj7 \| SCENE= }}~~	+	Structural characterization of the molecular platform for type III secretion system assembly.,Yip CK, Kimbrough TG, Felise HB, Vuckovic M, Thomas NA, Pfuetzner RA, Frey EA, Finlay BB, Miller SI, Strynadka NC Nature. 2005 Jun 2;435(7042):702-7. PMID:15931226<ref>PMID:15931226</ref>

-	~~===Crystal structure~~ of ~~enteropathogenic E~~.~~coli (EPEC) type III secretion system protein EscJ===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_15931226}}~~	+	== References ==
-		+	<references/>
-	~~==About this Structure==~~	+	__TOC__
-	~~[[1yj7]] is a 4 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org~~/~~wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YJ7 OCA].~~	+	</StructureSection>
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:015931226<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
	[[Category: Felise, H B.]]		[[Category: Felise, H B.]]

OCA: Protected "1yj7" [edit=sysop:move=sysop]

OCA — Sat, 05 Jan 2013 17:47:58 GMT

Protected "1yj7" [edit=sysop:move=sysop]

←Older revision

Revision as of 17:47, 5 January 2013

OCA at 17:47, 5 January 2013

OCA — Sat, 05 Jan 2013 17:47:56 GMT

←Older revision		Revision as of 17:47, 5 January 2013
Line 1:		Line 1:
-	{{Seed}}
	[[Image:1yj7.png\|left\|200px]]		[[Image:1yj7.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_1yj7", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_1yj7\| PDB=1yj7 \| SCENE= }}		{{STRUCTURE_1yj7\| PDB=1yj7 \| SCENE= }}

	===Crystal structure of enteropathogenic E.coli (EPEC) type III secretion system protein EscJ===		===Crystal structure of enteropathogenic E.coli (EPEC) type III secretion system protein EscJ===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_15931226}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 15931226 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_15931226}}		{{ABSTRACT_PUBMED_15931226}}

	==About this Structure==		==About this Structure==
-	~~1YJ7~~ is a 4 ~~chains~~ structure ~~of sequences~~ from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YJ7 OCA].	+	[[1yj7]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YJ7 OCA].

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~15931226~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:015931226</ref><references group="xtra"/>
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
	[[Category: Felise, H B.]]		[[Category: Felise, H B.]]
Line 37:		Line 25:
	[[Category: Extended linker]]		[[Category: Extended linker]]
	[[Category: Mixed alpha/beta]]		[[Category: Mixed alpha/beta]]
-		+	[[Category: Protein transport]]
-	~~''Page seeded by~~ [~~http~~:~~//oca.weizmann.ac.il/oca OCA~~ ] ~~on Tue Feb 17 10:21:19 2009''~~	+