1yns - Revision history

OCA at 13:38, 13 March 2024

OCA — Wed, 13 Mar 2024 13:38:19 GMT

←Older revision		Revision as of 13:38, 13 March 2024
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	<StructureSection load='1yns' size='340' side='right'caption='[[1yns]], [[Resolution\|resolution]] 1.70Å' scene=''>		<StructureSection load='1yns' size='340' side='right'caption='[[1yns]], [[Resolution\|resolution]] 1.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1yns]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Human Human~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YNS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1YNS FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1yns]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YNS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1YNS FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=HPO:2~~-~~OXOHEPTYLPHOSPHONIC+ACID'>HPO</scene>~~, ~~<scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.7Å</td></tr>
-	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"><~~div style~~='~~overflow~~: ~~auto; max~~-~~height~~: ~~3em;~~'>~~[[1wdh\|1wdh]]~~</~~div~~></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HPO:2-OXOHEPTYLPHOSPHONIC+ACID'>HPO</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1yns FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1yns OCA], [https://pdbe.org/1yns PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1yns RCSB], [https://www.ebi.ac.uk/pdbsum/1yns PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1yns ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1yns FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1yns OCA], [https://pdbe.org/1yns PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1yns RCSB], [https://www.ebi.ac.uk/pdbsum/1yns PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1yns ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/ENOPH_HUMAN ENOPH_HUMAN]] Bifunctional enzyme that catalyzes the enolization of 2,3-diketo-5-methylthiopentyl-1-phosphate (DK-MTP-1-P) into the intermediate 2-hydroxy-3-keto-5-methylthiopentenyl-1-phosphate (HK-MTPenyl-1-P), which is then dephosphorylated to form the acireductone 1,2-dihydroxy-3-keto-5-methylthiopentene (DHK-MTPene).<ref>PMID:15843022</ref>	+	[https://www.uniprot.org/uniprot/ENOPH_HUMAN ENOPH_HUMAN] Bifunctional enzyme that catalyzes the enolization of 2,3-diketo-5-methylthiopentyl-1-phosphate (DK-MTP-1-P) into the intermediate 2-hydroxy-3-keto-5-methylthiopentenyl-1-phosphate (HK-MTPenyl-1-P), which is then dephosphorylated to form the acireductone 1,2-dihydroxy-3-keto-5-methylthiopentene (DHK-MTPene).<ref>PMID:15843022</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 20:		Line 20:
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1yns ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1yns ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	Enolase-phosphatase E1 (MASA) is a bifunctional enzyme in the ubiquitous methionine salvage pathway that catalyzes the continuous reactions of 2,3-diketo-5-methylthio-1-phosphopentane to yield the aci-reductone metabolite using Mg2+ as cofactor. In this study, we have determined the crystal structure of MASA and its complex with a substrate analog to 1.7A resolution by multi-wavelength anomalous diffraction and molecular replacement techniques, respectively. The structures support the proposed mechanism of phosphatase activity and further suggest the probable mechanism of enolization. We establish a model for substrate binding to describe in detail the enzymatic reaction and the formation of the transition state, which will provide insight into the reaction mechanisms of other enzymes in the same family.
-
-	Crystal structure of human E1 enzyme and its complex with a substrate analog reveals the mechanism of its phosphatase/enolase activity.,Wang H, Pang H, Bartlam M, Rao Z J Mol Biol. 2005 May 13;348(4):917-26. PMID:15843022<ref>PMID:15843022</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 1yns" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Human~~]]	+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Bartlam, M]]	+	[[Category: Bartlam M]]
-	[[Category: Pang, H]]	+	[[Category: Pang H]]
-	[[Category: Rao, Z]]	+	[[Category: Rao Z]]
-	[[Category: Wang, H]]	+	[[Category: Wang H]]
-	~~[[Category: Hydrolase]]~~	+
-	~~[[Category: Hydrolase fold~~]]	+

OCA at 09:08, 21 April 2021

OCA — Wed, 21 Apr 2021 09:08:03 GMT

←Older revision		Revision as of 09:08, 21 April 2021
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	<StructureSection load='1yns' size='340' side='right'caption='[[1yns]], [[Resolution\|resolution]] 1.70Å' scene=''>		<StructureSection load='1yns' size='340' side='right'caption='[[1yns]], [[Resolution\|resolution]] 1.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1yns]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YNS OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1YNS FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1yns]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YNS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1YNS FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HPO:2-OXOHEPTYLPHOSPHONIC+ACID'>HPO</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HPO:2-OXOHEPTYLPHOSPHONIC+ACID'>HPO</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1wdh\|1wdh]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1wdh\|1wdh]]</div></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1yns FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1yns OCA], [~~http~~://pdbe.org/1yns PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1yns RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1yns PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1yns ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1yns FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1yns OCA], [https://pdbe.org/1yns PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1yns RCSB], [https://www.ebi.ac.uk/pdbsum/1yns PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1yns ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/ENOPH_HUMAN ENOPH_HUMAN]] Bifunctional enzyme that catalyzes the enolization of 2,3-diketo-5-methylthiopentyl-1-phosphate (DK-MTP-1-P) into the intermediate 2-hydroxy-3-keto-5-methylthiopentenyl-1-phosphate (HK-MTPenyl-1-P), which is then dephosphorylated to form the acireductone 1,2-dihydroxy-3-keto-5-methylthiopentene (DHK-MTPene).<ref>PMID:15843022</ref>	+	[[https://www.uniprot.org/uniprot/ENOPH_HUMAN ENOPH_HUMAN]] Bifunctional enzyme that catalyzes the enolization of 2,3-diketo-5-methylthiopentyl-1-phosphate (DK-MTP-1-P) into the intermediate 2-hydroxy-3-keto-5-methylthiopentenyl-1-phosphate (HK-MTPenyl-1-P), which is then dephosphorylated to form the acireductone 1,2-dihydroxy-3-keto-5-methylthiopentene (DHK-MTPene).<ref>PMID:15843022</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 13:26, 17 July 2019

OCA — Wed, 17 Jul 2019 13:26:35 GMT

←Older revision		Revision as of 13:26, 17 July 2019
Line 1:		Line 1:

	==Crystal Structure Of Human Enolase-phosphatase E1 and its complex with a substrate analog==		==Crystal Structure Of Human Enolase-phosphatase E1 and its complex with a substrate analog==
-	<StructureSection load='1yns' size='340' side='right' caption='[[1yns]], [[Resolution\|resolution]] 1.70Å' scene=''>	+	<StructureSection load='1yns' size='340' side='right'caption='[[1yns]], [[Resolution\|resolution]] 1.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1yns]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YNS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1YNS FirstGlance]. <br>		<table><tr><td colspan='2'>[[1yns]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YNS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1YNS FirstGlance]. <br>
Line 14:		Line 14:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/yn/1yns_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/yn/1yns_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
Line 34:		Line 34:
	</StructureSection>		</StructureSection>
	[[Category: Human]]		[[Category: Human]]
		+	[[Category: Large Structures]]
	[[Category: Bartlam, M]]		[[Category: Bartlam, M]]
	[[Category: Pang, H]]		[[Category: Pang, H]]

OCA at 16:01, 12 October 2017

OCA — Thu, 12 Oct 2017 16:01:29 GMT

←Older revision		Revision as of 16:01, 12 October 2017
Line 1:		Line 1:
		+
	==Crystal Structure Of Human Enolase-phosphatase E1 and its complex with a substrate analog==		==Crystal Structure Of Human Enolase-phosphatase E1 and its complex with a substrate analog==
	<StructureSection load='1yns' size='340' side='right' caption='[[1yns]], [[Resolution\|resolution]] 1.70Å' scene=''>		<StructureSection load='1yns' size='340' side='right' caption='[[1yns]], [[Resolution\|resolution]] 1.70Å' scene=''>
Line 5:		Line 6:
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HPO:2-OXOHEPTYLPHOSPHONIC+ACID'>HPO</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HPO:2-OXOHEPTYLPHOSPHONIC+ACID'>HPO</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1wdh\|1wdh]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1wdh\|1wdh]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1yns FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1yns OCA], [http://pdbe.org/1yns PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1yns RCSB], [http://www.ebi.ac.uk/pdbsum/1yns PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1yns FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1yns OCA], [http://pdbe.org/1yns PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1yns RCSB], [http://www.ebi.ac.uk/pdbsum/1yns PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1yns ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==

OCA at 01:47, 10 February 2016

OCA — Wed, 10 Feb 2016 01:47:28 GMT

←Older revision		Revision as of 01:47, 10 February 2016
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	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1yns ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 04:09, 11 September 2015

OCA — Fri, 11 Sep 2015 04:09:43 GMT

←Older revision		Revision as of 04:09, 11 September 2015
Line 2:		Line 2:
	<StructureSection load='1yns' size='340' side='right' caption='[[1yns]], [[Resolution\|resolution]] 1.70Å' scene=''>		<StructureSection load='1yns' size='340' side='right' caption='[[1yns]], [[Resolution\|resolution]] 1.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1yns]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Homo_sapiens Homo sapiens~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YNS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1YNS FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1yns]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YNS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1YNS FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HPO:2-OXOHEPTYLPHOSPHONIC+ACID'>HPO</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HPO:2-OXOHEPTYLPHOSPHONIC+ACID'>HPO</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1wdh\|1wdh]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1wdh\|1wdh]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1yns FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1yns OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1yns RCSB], [http://www.ebi.ac.uk/pdbsum/1yns PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1yns FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1yns OCA], [http://pdbe.org/1yns PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1yns RCSB], [http://www.ebi.ac.uk/pdbsum/1yns PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
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	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1yns" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Homo sapiens~~]]	+	[[Category: Human]]
	[[Category: Bartlam, M]]		[[Category: Bartlam, M]]
	[[Category: Pang, H]]		[[Category: Pang, H]]

OCA at 10:56, 25 December 2014

OCA — Thu, 25 Dec 2014 10:56:59 GMT

←Older revision		Revision as of 10:56, 25 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1yns]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YNS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1YNS FirstGlance]. <br>		<table><tr><td colspan='2'>[[1yns]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YNS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1YNS FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HPO:2-OXOHEPTYLPHOSPHONIC+ACID'>HPO</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HPO:2-OXOHEPTYLPHOSPHONIC+ACID'>HPO</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1wdh\|1wdh]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1wdh\|1wdh]]</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1yns FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1yns OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1yns RCSB], [http://www.ebi.ac.uk/pdbsum/1yns PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1yns FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1yns OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1yns RCSB], [http://www.ebi.ac.uk/pdbsum/1yns PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/ENOPH_HUMAN ENOPH_HUMAN]] Bifunctional enzyme that catalyzes the enolization of 2,3-diketo-5-methylthiopentyl-1-phosphate (DK-MTP-1-P) into the intermediate 2-hydroxy-3-keto-5-methylthiopentenyl-1-phosphate (HK-MTPenyl-1-P), which is then dephosphorylated to form the acireductone 1,2-dihydroxy-3-keto-5-methylthiopentene (DHK-MTPene).<ref>PMID:15843022</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 30:		Line 32:
	</StructureSection>		</StructureSection>
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
-	[[Category: Bartlam, M.]]	+	[[Category: Bartlam, M]]
-	[[Category: Pang, H.]]	+	[[Category: Pang, H]]
-	[[Category: Rao, Z.]]	+	[[Category: Rao, Z]]
-	[[Category: Wang, H.]]	+	[[Category: Wang, H]]
	[[Category: Hydrolase]]		[[Category: Hydrolase]]
	[[Category: Hydrolase fold]]		[[Category: Hydrolase fold]]

OCA at 21:58, 29 September 2014

OCA — Mon, 29 Sep 2014 21:58:24 GMT

←Older revision		Revision as of 21:58, 29 September 2014
Line 1:		Line 1:
-	[[~~Image:~~1yns.~~png~~\|~~left~~\|200px]]	+	==Crystal Structure Of Human Enolase-phosphatase E1 and its complex with a substrate analog==
		+	<StructureSection load='1yns' size='340' side='right' caption='[[1yns]], [[Resolution\|resolution]] 1.70Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1yns]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YNS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1YNS FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HPO:2-OXOHEPTYLPHOSPHONIC+ACID'>HPO</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1wdh\|1wdh]]</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1yns FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1yns OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1yns RCSB], [http://www.ebi.ac.uk/pdbsum/1yns PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/yn/1yns_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Enolase-phosphatase E1 (MASA) is a bifunctional enzyme in the ubiquitous methionine salvage pathway that catalyzes the continuous reactions of 2,3-diketo-5-methylthio-1-phosphopentane to yield the aci-reductone metabolite using Mg2+ as cofactor. In this study, we have determined the crystal structure of MASA and its complex with a substrate analog to 1.7A resolution by multi-wavelength anomalous diffraction and molecular replacement techniques, respectively. The structures support the proposed mechanism of phosphatase activity and further suggest the probable mechanism of enolization. We establish a model for substrate binding to describe in detail the enzymatic reaction and the formation of the transition state, which will provide insight into the reaction mechanisms of other enzymes in the same family.

-	~~{{STRUCTURE_1yns\| PDB=1yns \| SCENE= }}~~	+	Crystal structure of human E1 enzyme and its complex with a substrate analog reveals the mechanism of its phosphatase/enolase activity.,Wang H, Pang H, Bartlam M, Rao Z J Mol Biol. 2005 May 13;348(4):917-26. PMID:15843022<ref>PMID:15843022</ref>

-	~~===Crystal Structure Of Human Enolase-phosphatase E1 and its complex with~~ a ~~substrate analog===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_15843022}}~~	+	== References ==
-		+	<references/>
-	~~==About this Structure==~~	+	__TOC__
-	~~[[1yns]] is a 1 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Homo_sapiens Homo sapiens]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=1YNS OCA].~~	+	</StructureSection>
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:015843022<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
	[[Category: Bartlam, M.]]		[[Category: Bartlam, M.]]

OCA: Protected "1yns" [edit=sysop:move=sysop]

OCA — Sat, 05 Jan 2013 17:32:14 GMT

Protected "1yns" [edit=sysop:move=sysop]

←Older revision

Revision as of 17:32, 5 January 2013

OCA at 17:32, 5 January 2013

OCA — Sat, 05 Jan 2013 17:32:11 GMT

←Older revision		Revision as of 17:32, 5 January 2013
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-	{{Seed}}
	[[Image:1yns.png\|left\|200px]]		[[Image:1yns.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_1yns", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_1yns\| PDB=1yns \| SCENE= }}		{{STRUCTURE_1yns\| PDB=1yns \| SCENE= }}

	===Crystal Structure Of Human Enolase-phosphatase E1 and its complex with a substrate analog===		===Crystal Structure Of Human Enolase-phosphatase E1 and its complex with a substrate analog===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_15843022}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 15843022 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_15843022}}		{{ABSTRACT_PUBMED_15843022}}

	==About this Structure==		==About this Structure==
-	~~1YNS~~ is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YNS OCA].	+	[[1yns]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YNS OCA].

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~15843022~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:015843022</ref><references group="xtra"/>
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
	[[Category: Bartlam, M.]]		[[Category: Bartlam, M.]]
Line 29:		Line 17:
	[[Category: Rao, Z.]]		[[Category: Rao, Z.]]
	[[Category: Wang, H.]]		[[Category: Wang, H.]]
		+	[[Category: Hydrolase]]
	[[Category: Hydrolase fold]]		[[Category: Hydrolase fold]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 14:26:01 2009''