1yrt - Revision history

OCA at 08:59, 14 February 2024

OCA — Wed, 14 Feb 2024 08:59:19 GMT

←Older revision		Revision as of 08:59, 14 February 2024
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	<StructureSection load='1yrt' size='340' side='right'caption='[[1yrt]], [[Resolution\|resolution]] 2.10Å' scene=''>		<StructureSection load='1yrt' size='340' side='right'caption='[[1yrt]], [[Resolution\|resolution]] 2.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1yrt]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~"bacterium_tussis-convulsivae"_lehmann_and_neumann_1927 "bacterium tussis-convulsivae" lehmann and neumann 1927~~] and [~~http~~://en.wikipedia.org/wiki/~~Human Human~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YRT OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=1YRT FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1yrt]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bordetella_pertussis Bordetella pertussis] and [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YRT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1YRT FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.1Å</td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>~~[[~~Related_structure~~\|~~Related:~~]]~~</b></td><td class="sblockDat"><div style='overflow: auto~~; ~~max-height: 3em;'>[[1yru\|1yru]]</div>~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat"~~>cya, cyaA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&~~id=~~520~~ "~~Bacterium tussis-convulsivae~~" ~~Lehmann and Neumann 1927]), CALM1, CALM2, CALM3 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td~~><~~/tr>~~	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1yrt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1yrt OCA], [https://pdbe.org/1yrt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1yrt RCSB], [https://www.ebi.ac.uk/pdbsum/1yrt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1yrt ProSAT]</span></td></tr>
-	~~<tr id~~='~~activity'><td class~~=~~"sblockLbl"><b>Activity~~:~~</b></td><td class="sblockDat"><span class='plainlinks~~'>~~[http://en.wikipedia.org/wiki/Adenylate_cyclase Adenylate cyclase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.6.1.1 4.6.1.1]~~ </~~span~~></td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=1yrt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1yrt OCA], [~~http~~://pdbe.org/1yrt PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1yrt RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1yrt PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1yrt ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/CYAA_BORPE CYAA_BORPE]] This adenylate cyclase belongs to a special class of bacterial toxin. It causes whooping cough by acting on mammalian cells by elevating cAMP-concentration and thus disrupts normal cell function.	+	[https://www.uniprot.org/uniprot/CYAA_BORPE CYAA_BORPE] This adenylate cyclase belongs to a special class of bacterial toxin. It causes whooping cough by acting on mammalian cells by elevating cAMP-concentration and thus disrupts normal cell function.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1yrt ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1yrt ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	CyaA is crucial for colonization by Bordetella pertussis, the etiologic agent of whooping cough. Here we report crystal structures of the adenylyl cyclase domain (ACD) of CyaA with the C-terminal domain of calmodulin. Four discrete regions of CyaA bind calcium-loaded calmodulin with a large buried contact surface. Of those, a tryptophan residue (W242) at an alpha-helix of CyaA makes extensive contacts with the calcium-induced, hydrophobic pocket of calmodulin. Mutagenic analyses show that all four regions of CyaA contribute to calmodulin binding and the calmodulin-induced conformational change of CyaA is crucial for catalytic activation. A crystal structure of CyaA-calmodulin with adefovir diphosphate, the metabolite of an approved antiviral drug, reveals the location of catalytic site of CyaA and how adefovir diphosphate tightly binds CyaA. The ACD of CyaA shares a similar structure and mechanism of activation with anthrax edema factor (EF). However, the interactions of CyaA with calmodulin completely diverge from those of EF. This provides molecular details of how two structurally homologous bacterial toxins evolved divergently to bind calmodulin, an evolutionarily conserved calcium sensor.
-
-	Structural basis for the interaction of Bordetella pertussis adenylyl cyclase toxin with calmodulin.,Guo Q, Shen Y, Lee YS, Gibbs CS, Mrksich M, Tang WJ EMBO J. 2005 Sep 21;24(18):3190-201. Epub 2005 Sep 1. PMID:16138079<ref>PMID:16138079</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 1yrt" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
	*[[Calmodulin 3D structures\|Calmodulin 3D structures]]		*[[Calmodulin 3D structures\|Calmodulin 3D structures]]
	*[[3D Adenylyl cyclase 3D structures\|3D Adenylyl cyclase 3D structures]]		*[[3D Adenylyl cyclase 3D structures\|3D Adenylyl cyclase 3D structures]]
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Bacterium tussis-convulsivae lehmann and neumann 1927~~]]	+	[[Category: Bordetella pertussis]]
-	[[Category: ~~Adenylate cyclase]]~~	+	[[Category: Homo sapiens]]
-	~~[[Category: Human~~]]	+
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Gibbs~~, C S~~]]	+	[[Category: Gibbs CS]]
-	[[Category: Guo, Q]]	+	[[Category: Guo Q]]
-	[[Category: Lee~~, Y S~~]]	+	[[Category: Lee YS]]
-	[[Category: Mrksich, M]]	+	[[Category: Mrksich M]]
-	[[Category: Shen, Y]]	+	[[Category: Shen Y]]
-	[[Category: Tang~~, W J]]~~	+	[[Category: Tang WJ]]
-	~~[[Category: Cam]]~~	+
-	~~[[Category: Cyaa]]~~	+
-	~~[[Category: Layse]]~~	+
-	~~[[Category: Toxin~~]]	+

OCA at 08:51, 20 January 2021

OCA — Wed, 20 Jan 2021 08:51:13 GMT

←Older revision		Revision as of 08:51, 20 January 2021
Line 1:		Line 1:

	==Crystal Structure analysis of the adenylyl cyclaes catalytic domain of adenylyl cyclase toxin of Bordetella pertussis in presence of c-terminal calmodulin==		==Crystal Structure analysis of the adenylyl cyclaes catalytic domain of adenylyl cyclase toxin of Bordetella pertussis in presence of c-terminal calmodulin==
-	<StructureSection load='1yrt' size='340' side='right' caption='[[1yrt]], [[Resolution\|resolution]] 2.10Å' scene=''>	+	<StructureSection load='1yrt' size='340' side='right'caption='[[1yrt]], [[Resolution\|resolution]] 2.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1yrt]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacterium_tussis-convulsivae"_lehmann_and_neumann_1927 "bacterium tussis-convulsivae" lehmann and neumann 1927] and [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YRT OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1YRT FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1yrt]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacterium_tussis-convulsivae"_lehmann_and_neumann_1927 "bacterium tussis-convulsivae" lehmann and neumann 1927] and [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YRT OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=1YRT FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1yru\|1yru]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1yru\|1yru]]</div></td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">cya, cyaA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=520 "Bacterium tussis-convulsivae" Lehmann and Neumann 1927]), CALM1, CALM2, CALM3 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">cya, cyaA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=520 "Bacterium tussis-convulsivae" Lehmann and Neumann 1927]), CALM1, CALM2, CALM3 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Adenylate_cyclase Adenylate cyclase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.6.1.1 4.6.1.1] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Adenylate_cyclase Adenylate cyclase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.6.1.1 4.6.1.1] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1yrt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1yrt OCA], [http://pdbe.org/1yrt PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1yrt RCSB], [http://www.ebi.ac.uk/pdbsum/1yrt PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1yrt ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=1yrt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1yrt OCA], [http://pdbe.org/1yrt PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1yrt RCSB], [http://www.ebi.ac.uk/pdbsum/1yrt PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1yrt ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
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	==See Also==		==See Also==
-	*[[~~Adenylyl cyclase~~\|~~Adenylyl cyclase~~]]	+	*[[Calmodulin 3D structures\|Calmodulin 3D structures]]
-	*[[~~Calmodulin~~\|~~Calmodulin~~]]	+	*[[3D Adenylyl cyclase 3D structures\|3D Adenylyl cyclase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
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	[[Category: Adenylate cyclase]]		[[Category: Adenylate cyclase]]
	[[Category: Human]]		[[Category: Human]]
		+	[[Category: Large Structures]]
	[[Category: Gibbs, C S]]		[[Category: Gibbs, C S]]
	[[Category: Guo, Q]]		[[Category: Guo, Q]]

OCA at 06:47, 25 April 2018

OCA — Wed, 25 Apr 2018 06:47:37 GMT

←Older revision		Revision as of 06:47, 25 April 2018
Line 1:		Line 1:
		+
	==Crystal Structure analysis of the adenylyl cyclaes catalytic domain of adenylyl cyclase toxin of Bordetella pertussis in presence of c-terminal calmodulin==		==Crystal Structure analysis of the adenylyl cyclaes catalytic domain of adenylyl cyclase toxin of Bordetella pertussis in presence of c-terminal calmodulin==
	<StructureSection load='1yrt' size='340' side='right' caption='[[1yrt]], [[Resolution\|resolution]] 2.10Å' scene=''>		<StructureSection load='1yrt' size='340' side='right' caption='[[1yrt]], [[Resolution\|resolution]] 2.10Å' scene=''>
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	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">cya, cyaA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=520 "Bacterium tussis-convulsivae" Lehmann and Neumann 1927]), CALM1, CALM2, CALM3 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">cya, cyaA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=520 "Bacterium tussis-convulsivae" Lehmann and Neumann 1927]), CALM1, CALM2, CALM3 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Adenylate_cyclase Adenylate cyclase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.6.1.1 4.6.1.1] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Adenylate_cyclase Adenylate cyclase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.6.1.1 4.6.1.1] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1yrt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1yrt OCA], [http://pdbe.org/1yrt PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1yrt RCSB], [http://www.ebi.ac.uk/pdbsum/1yrt PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1yrt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1yrt OCA], [http://pdbe.org/1yrt PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1yrt RCSB], [http://www.ebi.ac.uk/pdbsum/1yrt PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1yrt ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 15:		Line 16:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/yr/1yrt_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/yr/1yrt_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1yrt ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 16:07, 11 September 2015

OCA — Fri, 11 Sep 2015 16:07:34 GMT

←Older revision		Revision as of 16:07, 11 September 2015
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	<StructureSection load='1yrt' size='340' side='right' caption='[[1yrt]], [[Resolution\|resolution]] 2.10Å' scene=''>		<StructureSection load='1yrt' size='340' side='right' caption='[[1yrt]], [[Resolution\|resolution]] 2.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1yrt]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Bordetella_pertussis Bordetella pertussis~~] and [http://en.wikipedia.org/wiki/~~Homo_sapiens Homo sapiens~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YRT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1YRT FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1yrt]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacterium_tussis-convulsivae"_lehmann_and_neumann_1927 "bacterium tussis-convulsivae" lehmann and neumann 1927] and [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YRT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1YRT FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1yru\|1yru]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1yru\|1yru]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">cya, cyaA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=520 ~~Bordetella pertussis~~]), CALM1, CALM2, CALM3 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 ~~Homo sapiens~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">cya, cyaA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=520 "Bacterium tussis-convulsivae" Lehmann and Neumann 1927]), CALM1, CALM2, CALM3 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Adenylate_cyclase Adenylate cyclase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.6.1.1 4.6.1.1] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Adenylate_cyclase Adenylate cyclase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.6.1.1 4.6.1.1] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1yrt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1yrt OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1yrt RCSB], [http://www.ebi.ac.uk/pdbsum/1yrt PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1yrt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1yrt OCA], [http://pdbe.org/1yrt PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1yrt RCSB], [http://www.ebi.ac.uk/pdbsum/1yrt PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 29:		Line 29:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1yrt" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 37:		Line 38:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Bacterium tussis-convulsivae lehmann and neumann 1927]]
	[[Category: Adenylate cyclase]]		[[Category: Adenylate cyclase]]
-	[[Category: ~~Bordetella pertussis]]~~	+	[[Category: Human]]
-	~~[[Category: Homo sapiens~~]]	+
	[[Category: Gibbs, C S]]		[[Category: Gibbs, C S]]
	[[Category: Guo, Q]]		[[Category: Guo, Q]]

OCA at 18:48, 25 December 2014

OCA — Thu, 25 Dec 2014 18:48:36 GMT

←Older revision		Revision as of 18:48, 25 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1yrt]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bordetella_pertussis Bordetella pertussis] and [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YRT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1YRT FirstGlance]. <br>		<table><tr><td colspan='2'>[[1yrt]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bordetella_pertussis Bordetella pertussis] and [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YRT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1YRT FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1yru\|1yru]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1yru\|1yru]]</td></tr>
-	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">cya, cyaA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=520 Bordetella pertussis]), CALM1, CALM2, CALM3 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">cya, cyaA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=520 Bordetella pertussis]), CALM1, CALM2, CALM3 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])</td></tr>
-	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Adenylate_cyclase Adenylate cyclase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.6.1.1 4.6.1.1] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Adenylate_cyclase Adenylate cyclase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.6.1.1 4.6.1.1] </span></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1yrt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1yrt OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1yrt RCSB], [http://www.ebi.ac.uk/pdbsum/1yrt PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1yrt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1yrt OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1yrt RCSB], [http://www.ebi.ac.uk/pdbsum/1yrt PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/CYAA_BORPE CYAA_BORPE]] This adenylate cyclase belongs to a special class of bacterial toxin. It causes whooping cough by acting on mammalian cells by elevating cAMP-concentration and thus disrupts normal cell function.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 38:		Line 40:
	[[Category: Bordetella pertussis]]		[[Category: Bordetella pertussis]]
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
-	[[Category: Gibbs, C S.]]	+	[[Category: Gibbs, C S]]
-	[[Category: Guo, Q.]]	+	[[Category: Guo, Q]]
-	[[Category: Lee, Y S.]]	+	[[Category: Lee, Y S]]
-	[[Category: Mrksich, M.]]	+	[[Category: Mrksich, M]]
-	[[Category: Shen, Y.]]	+	[[Category: Shen, Y]]
-	[[Category: Tang, W J.]]	+	[[Category: Tang, W J]]
	[[Category: Cam]]		[[Category: Cam]]
	[[Category: Cyaa]]		[[Category: Cyaa]]
	[[Category: Layse]]		[[Category: Layse]]
	[[Category: Toxin]]		[[Category: Toxin]]

OCA at 23:58, 28 September 2014

OCA — Sun, 28 Sep 2014 23:58:43 GMT

←Older revision		Revision as of 23:58, 28 September 2014
Line 1:		Line 1:
-	[[~~Image:~~1yrt.~~png~~\|~~left~~\|200px]]	+	==Crystal Structure analysis of the adenylyl cyclaes catalytic domain of adenylyl cyclase toxin of Bordetella pertussis in presence of c-terminal calmodulin==
		+	<StructureSection load='1yrt' size='340' side='right' caption='[[1yrt]], [[Resolution\|resolution]] 2.10Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1yrt]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bordetella_pertussis Bordetella pertussis] and [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YRT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1YRT FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1yru\|1yru]]</td></tr>
		+	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">cya, cyaA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=520 Bordetella pertussis]), CALM1, CALM2, CALM3 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])</td></tr>
		+	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Adenylate_cyclase Adenylate cyclase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.6.1.1 4.6.1.1] </span></td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1yrt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1yrt OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1yrt RCSB], [http://www.ebi.ac.uk/pdbsum/1yrt PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/yr/1yrt_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	CyaA is crucial for colonization by Bordetella pertussis, the etiologic agent of whooping cough. Here we report crystal structures of the adenylyl cyclase domain (ACD) of CyaA with the C-terminal domain of calmodulin. Four discrete regions of CyaA bind calcium-loaded calmodulin with a large buried contact surface. Of those, a tryptophan residue (W242) at an alpha-helix of CyaA makes extensive contacts with the calcium-induced, hydrophobic pocket of calmodulin. Mutagenic analyses show that all four regions of CyaA contribute to calmodulin binding and the calmodulin-induced conformational change of CyaA is crucial for catalytic activation. A crystal structure of CyaA-calmodulin with adefovir diphosphate, the metabolite of an approved antiviral drug, reveals the location of catalytic site of CyaA and how adefovir diphosphate tightly binds CyaA. The ACD of CyaA shares a similar structure and mechanism of activation with anthrax edema factor (EF). However, the interactions of CyaA with calmodulin completely diverge from those of EF. This provides molecular details of how two structurally homologous bacterial toxins evolved divergently to bind calmodulin, an evolutionarily conserved calcium sensor.

-	~~{{STRUCTURE_1yrt\| PDB=1yrt \| SCENE= }}~~	+	Structural basis for the interaction of Bordetella pertussis adenylyl cyclase toxin with calmodulin.,Guo Q, Shen Y, Lee YS, Gibbs CS, Mrksich M, Tang WJ EMBO J. 2005 Sep 21;24(18):3190-201. Epub 2005 Sep 1. PMID:16138079<ref>PMID:16138079</ref>

-	~~===Crystal Structure analysis~~ of the ~~adenylyl cyclaes catalytic domain of adenylyl cyclase toxin of Bordetella pertussis in presence of c-terminal calmodulin===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_16138079}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[1yrt]] is a 2 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Bordetella_pertussis Bordetella pertussis] and [http://en~~.~~wikipedia.org~~/~~wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YRT OCA].~~	+

	==See Also==		==See Also==
	*[[Adenylyl cyclase\|Adenylyl cyclase]]		*[[Adenylyl cyclase\|Adenylyl cyclase]]
	*[[Calmodulin\|Calmodulin]]		*[[Calmodulin\|Calmodulin]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	<~~ref group="xtra">PMID:016138079<~~/~~ref~~><~~references group="xtra"~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Adenylate cyclase]]		[[Category: Adenylate cyclase]]
	[[Category: Bordetella pertussis]]		[[Category: Bordetella pertussis]]

OCA at 20:10, 20 October 2012

OCA — Sat, 20 Oct 2012 20:10:13 GMT

←Older revision		Revision as of 20:10, 20 October 2012
Line 8:		Line 8:

	==About this Structure==		==About this Structure==
-	[[1yrt]] is a 2 chain structure ~~of [[Adenylyl cyclase]] and [[Calmodulin]]~~ with sequence from [http://en.wikipedia.org/wiki/Bordetella_pertussis Bordetella pertussis] and [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YRT OCA].	+	[[1yrt]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bordetella_pertussis Bordetella pertussis] and [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YRT OCA].

	==See Also==		==See Also==

OCA: Protected "1yrt" [edit=sysop:move=sysop]

OCA — Fri, 27 Jul 2012 09:37:58 GMT

Protected "1yrt" [edit=sysop:move=sysop]

←Older revision

Revision as of 09:37, 27 July 2012

OCA at 09:37, 27 July 2012

OCA — Fri, 27 Jul 2012 09:37:55 GMT

←Older revision		Revision as of 09:37, 27 July 2012
Line 1:		Line 1:
-	{{Seed}}
	[[Image:1yrt.png\|left\|200px]]		[[Image:1yrt.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_1yrt", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_1yrt\| PDB=1yrt \| SCENE= }}		{{STRUCTURE_1yrt\| PDB=1yrt \| SCENE= }}

	===Crystal Structure analysis of the adenylyl cyclaes catalytic domain of adenylyl cyclase toxin of Bordetella pertussis in presence of c-terminal calmodulin===		===Crystal Structure analysis of the adenylyl cyclaes catalytic domain of adenylyl cyclase toxin of Bordetella pertussis in presence of c-terminal calmodulin===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_16138079}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 16138079 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_16138079}}		{{ABSTRACT_PUBMED_16138079}}

	==About this Structure==		==About this Structure==
-	~~1YRT~~ is a 2 ~~chains~~ structure of ~~sequences~~ from [http://en.wikipedia.org/wiki/Bordetella_pertussis Bordetella pertussis] and [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YRT OCA].	+	[[1yrt]] is a 2 chain structure of [[Adenylyl cyclase]] and [[Calmodulin]] with sequence from [http://en.wikipedia.org/wiki/Bordetella_pertussis Bordetella pertussis] and [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YRT OCA].
		+
		+	==See Also==
		+	*[[Adenylyl cyclase\|Adenylyl cyclase]]
		+	*[[Calmodulin\|Calmodulin]]

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~16138079~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:016138079</ref><references group="xtra"/>
	[[Category: Adenylate cyclase]]		[[Category: Adenylate cyclase]]
	[[Category: Bordetella pertussis]]		[[Category: Bordetella pertussis]]
Line 35:		Line 27:
	[[Category: Cam]]		[[Category: Cam]]
	[[Category: Cyaa]]		[[Category: Cyaa]]
-		+	[[Category: Layse]]
-	~~''Page seeded by~~ [~~http~~:~~//oca.weizmann.ac.il/oca OCA~~ ] ~~on Wed Feb 18 02~~:~~59:06 2009''~~	+	[[Category: Toxin]]

OCA at 00:59, 18 February 2009

OCA — Wed, 18 Feb 2009 00:59:07 GMT

←Older revision		Revision as of 00:59, 18 February 2009
Line 20:		Line 20:

	==About this Structure==		==About this Structure==
-	1YRT is a ~~[[Protein complex]]~~ structure of sequences from [http://en.wikipedia.org/wiki/Bordetella_pertussis Bordetella pertussis] and [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YRT OCA].	+	1YRT is a 2 chains structure of sequences from [http://en.wikipedia.org/wiki/Bordetella_pertussis Bordetella pertussis] and [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YRT OCA].

	==Reference==		==Reference==
-	Structural basis for the interaction of Bordetella pertussis adenylyl cyclase toxin with calmodulin., Guo Q, Shen Y, Lee YS, Gibbs CS, Mrksich M, Tang WJ, EMBO J. 2005 Sep 21;24(18):3190-201. Epub 2005 Sep 1. PMID:~~[http:~~//~~www.ncbi.nlm.nih.gov/pubmed/16138079 16138079]~~	+	<ref group="xtra">PMID:16138079</ref><references group="xtra"/>
	[[Category: Adenylate cyclase]]		[[Category: Adenylate cyclase]]
	[[Category: Bordetella pertussis]]		[[Category: Bordetella pertussis]]
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
-	[[Category: Protein complex]]
	[[Category: Gibbs, C S.]]		[[Category: Gibbs, C S.]]
	[[Category: Guo, Q.]]		[[Category: Guo, Q.]]
Line 37:		Line 36:
	[[Category: Cyaa]]		[[Category: Cyaa]]

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Tue Jul 29 09~~:39:~~27 2008~~''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Feb 18 02:59:06 2009''