1ytr - Revision history

OCA at 09:31, 6 December 2023

OCA — Wed, 06 Dec 2023 09:31:06 GMT

←Older revision		Revision as of 09:31, 6 December 2023
Line 1:		Line 1:

	==NMR structure of plantaricin a in dpc micelles, 20 structures==		==NMR structure of plantaricin a in dpc micelles, 20 structures==
-	<StructureSection load='1ytr' size='340' side='right'caption='[[1ytr~~]], [[NMR_Ensembles_of_Models \| 20 NMR models~~]]' scene=''>	+	<StructureSection load='1ytr' size='340' side='right'caption='[[1ytr]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1ytr]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YTR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1YTR FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1ytr]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Lactiplantibacillus_plantarum_WCFS1 Lactiplantibacillus plantarum WCFS1]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YTR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1YTR FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ytr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ytr OCA], [https://pdbe.org/1ytr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ytr RCSB], [https://www.ebi.ac.uk/pdbsum/1ytr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ytr ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ytr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ytr OCA], [https://pdbe.org/1ytr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ytr RCSB], [https://www.ebi.ac.uk/pdbsum/1ytr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ytr ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/PLNA_LACPL PLNA_LACPL]] This heat stable bacteriocin inhibits the growth of closely related Lactobacillus species. It may act as a pore-forming protein, creating a channel in the cell membrane through a "barrel stave" mechanism.	+	[https://www.uniprot.org/uniprot/PLNA_LACPL PLNA_LACPL] This heat stable bacteriocin inhibits the growth of closely related Lactobacillus species. It may act as a pore-forming protein, creating a channel in the cell membrane through a "barrel stave" mechanism.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 21:		Line 22:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Lactiplantibacillus plantarum WCFS1]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Fimland, G]]	+	[[Category: Fimland G]]
-	[[Category: Kristiansen~~, P E~~]]	+	[[Category: Kristiansen PE]]
-	[[Category: Mantzilas, D]]	+	[[Category: Mantzilas D]]
-	[[Category: Nissen-Meyer, J]]	+	[[Category: Nissen-Meyer J]]
-	~~[[Category: Amphipathic helix]]~~	+
-	~~[[Category: Antibiotic]]~~	+
-	~~[[Category: Micelle]]~~	+
-	~~[[Category: Pheromone~~]]	+

OCA at 16:32, 3 November 2021

OCA — Wed, 03 Nov 2021 16:32:46 GMT

←Older revision		Revision as of 16:32, 3 November 2021
Line 3:		Line 3:
	<StructureSection load='1ytr' size='340' side='right'caption='[[1ytr]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>		<StructureSection load='1ytr' size='340' side='right'caption='[[1ytr]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1ytr]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YTR OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1YTR FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1ytr]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YTR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1YTR FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1ytr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ytr OCA], [~~http~~://pdbe.org/1ytr PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1ytr RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1ytr PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1ytr ProSAT]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ytr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ytr OCA], [https://pdbe.org/1ytr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ytr RCSB], [https://www.ebi.ac.uk/pdbsum/1ytr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ytr ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/PLNA_LACPL PLNA_LACPL]] This heat stable bacteriocin inhibits the growth of closely related Lactobacillus species. It may act as a pore-forming protein, creating a channel in the cell membrane through a "barrel stave" mechanism.	+	[[https://www.uniprot.org/uniprot/PLNA_LACPL PLNA_LACPL]] This heat stable bacteriocin inhibits the growth of closely related Lactobacillus species. It may act as a pore-forming protein, creating a channel in the cell membrane through a "barrel stave" mechanism.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==

OCA at 17:15, 29 January 2020

OCA — Wed, 29 Jan 2020 17:15:51 GMT

←Older revision		Revision as of 17:15, 29 January 2020
Line 1:		Line 1:

	==NMR structure of plantaricin a in dpc micelles, 20 structures==		==NMR structure of plantaricin a in dpc micelles, 20 structures==
-	<StructureSection load='1ytr' size='340' side='right' caption='[[1ytr]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>	+	<StructureSection load='1ytr' size='340' side='right'caption='[[1ytr]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1ytr]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YTR OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1YTR FirstGlance]. <br>		<table><tr><td colspan='2'>[[1ytr]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YTR OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1YTR FirstGlance]. <br>
Line 21:		Line 21:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Fimland, G]]		[[Category: Fimland, G]]
	[[Category: Kristiansen, P E]]		[[Category: Kristiansen, P E]]

OCA at 06:58, 25 April 2018

OCA — Wed, 25 Apr 2018 06:58:42 GMT

←Older revision		Revision as of 06:58, 25 April 2018
Line 1:		Line 1:
		+
	==NMR structure of plantaricin a in dpc micelles, 20 structures==		==NMR structure of plantaricin a in dpc micelles, 20 structures==
	<StructureSection load='1ytr' size='340' side='right' caption='[[1ytr]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>		<StructureSection load='1ytr' size='340' side='right' caption='[[1ytr]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1ytr]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YTR OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1YTR FirstGlance]. <br>		<table><tr><td colspan='2'>[[1ytr]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YTR OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1YTR FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ytr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ytr OCA], [http://pdbe.org/1ytr PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1ytr RCSB], [http://www.ebi.ac.uk/pdbsum/1ytr PDBsum]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ytr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ytr OCA], [http://pdbe.org/1ytr PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1ytr RCSB], [http://www.ebi.ac.uk/pdbsum/1ytr PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1ytr ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==

OCA at 12:26, 11 September 2015

OCA — Fri, 11 Sep 2015 12:26:32 GMT

←Older revision		Revision as of 12:26, 11 September 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1ytr]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YTR OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1YTR FirstGlance]. <br>		<table><tr><td colspan='2'>[[1ytr]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YTR OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1YTR FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ytr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ytr OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1ytr RCSB], [http://www.ebi.ac.uk/pdbsum/1ytr PDBsum]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ytr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ytr OCA], [http://pdbe.org/1ytr PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1ytr RCSB], [http://www.ebi.ac.uk/pdbsum/1ytr PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 15:		Line 15:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1ytr" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 16:26, 25 December 2014

OCA — Thu, 25 Dec 2014 16:26:01 GMT

←Older revision		Revision as of 16:26, 25 December 2014
Line 5:		Line 5:
	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ytr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ytr OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1ytr RCSB], [http://www.ebi.ac.uk/pdbsum/1ytr PDBsum]</span></td></tr>		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ytr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ytr OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1ytr RCSB], [http://www.ebi.ac.uk/pdbsum/1ytr PDBsum]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/PLNA_LACPL PLNA_LACPL]] This heat stable bacteriocin inhibits the growth of closely related Lactobacillus species. It may act as a pore-forming protein, creating a channel in the cell membrane through a "barrel stave" mechanism.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 17:		Line 19:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Fimland, G.]]	+	[[Category: Fimland, G]]
-	[[Category: Kristiansen, P E.]]	+	[[Category: Kristiansen, P E]]
-	[[Category: Mantzilas, D.]]	+	[[Category: Mantzilas, D]]
-	[[Category: Nissen-Meyer, J.]]	+	[[Category: Nissen-Meyer, J]]
	[[Category: Amphipathic helix]]		[[Category: Amphipathic helix]]
	[[Category: Antibiotic]]		[[Category: Antibiotic]]
	[[Category: Micelle]]		[[Category: Micelle]]
	[[Category: Pheromone]]		[[Category: Pheromone]]

OCA at 08:27, 8 October 2014

OCA — Wed, 08 Oct 2014 08:27:00 GMT

←Older revision		Revision as of 08:27, 8 October 2014
Line 1:		Line 1:
-	[[~~Image~~:1ytr.~~png\|left\|200px~~]]	+	==NMR structure of plantaricin a in dpc micelles, 20 structures==
		+	<StructureSection load='1ytr' size='340' side='right' caption='[[1ytr]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1ytr]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YTR OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1YTR FirstGlance]. <br>
		+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ytr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ytr OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1ytr RCSB], [http://www.ebi.ac.uk/pdbsum/1ytr PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The three-dimensional structure in dodecyl phosphocholine micelles of the 26-mer membrane-permeabilizing bacteriocin-like pheromone plantaricin A (PlnA) has been determined by use of nuclear magnetic resonance spectroscopy. The peptide was unstructured in water but became partly structured upon exposure to micelles. An amphiphilic alpha-helix stretching from residue 12 to 21 (possibly also including residues 22 and 23) was then formed in the C-terminal part of the peptide, whereas the N-terminal part remained largely unstructured. PlnA exerted its membrane-permeabilizing antimicrobial activity through a nonchiral interaction with the target cell membrane because the d-enantiomeric form had the same activity as the natural l-form. This nonchiral interaction involved the amphiphilic alpha-helical region in the C-terminal half of PlnA because a 17-mer fragment that contains the amphiphilic alpha-helical part of the peptide had antimicrobial potency that was similar to that of the l- and d-enantiomeric forms of PlnA. Also the pheromone activity of PlnA depended on this nonchiral interaction because both the l- and d-enantiomeric forms of the 17-mer fragment inhibited the pheromone activity. The pheromone activity also involved, however, a chiral interaction between the N-terminal part of PlnA and its receptor because high concentrations of the l-form (but not the d-form) of a 5-mer fragment derived from the N-terminal part of PlnA had pheromone activity. The results thus reveal a novel mechanism whereby peptide pheromones such as PlnA may function. An initial nonchiral interaction with membrane lipids induces alpha-helical structuring in a segment of the peptide pheromone. The peptide becomes thereby sufficiently structured and properly positioned in the membrane interface, thus enabling it to engage in a chiral interaction with its receptor in or near the membrane water interface. This membrane-interacting mode of action explains why some peptide pheromones/hormones such as PlnA sometimes display antimicrobial activity in addition to their pheromone activity.

-	~~{{STRUCTURE_1ytr\| PDB=1ytr \| SCENE= }}~~	+	Structure and mode of action of the membrane-permeabilizing antimicrobial peptide pheromone plantaricin A.,Kristiansen PE, Fimland G, Mantzilas D, Nissen-Meyer J J Biol Chem. 2005 Jun 17;280(24):22945-50. Epub 2005 Apr 1. PMID:15805109<ref>PMID:15805109</ref>

-	~~===NMR structure~~ of ~~plantaricin a in dpc micelles, 20 structures===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_15805109}}~~	+	== References ==
-		+	<references/>
-	==~~About this Structure~~==	+	__TOC__
-	~~[[1ytr]] is a 1 chain structure. Full experimental information is available from [http:~~//~~oca.weizmann.ac.il/oca-bin/ocashort?id=1YTR OCA].~~	+	</StructureSection>
	[[Category: Fimland, G.]]		[[Category: Fimland, G.]]
	[[Category: Kristiansen, P E.]]		[[Category: Kristiansen, P E.]]

OCA: Protected "1ytr" [edit=sysop:move=sysop]

OCA — Sat, 05 Jan 2013 17:41:06 GMT

Protected "1ytr" [edit=sysop:move=sysop]

←Older revision

Revision as of 17:41, 5 January 2013

OCA at 17:41, 5 January 2013

OCA — Sat, 05 Jan 2013 17:41:04 GMT

←Older revision		Revision as of 17:41, 5 January 2013
Line 1:		Line 1:
-	{{Seed}}
	[[Image:1ytr.png\|left\|200px]]		[[Image:1ytr.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_1ytr", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_1ytr\| PDB=1ytr \| SCENE= }}		{{STRUCTURE_1ytr\| PDB=1ytr \| SCENE= }}

	===NMR structure of plantaricin a in dpc micelles, 20 structures===		===NMR structure of plantaricin a in dpc micelles, 20 structures===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_15805109}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 15805109 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_15805109}}		{{ABSTRACT_PUBMED_15805109}}

	==About this Structure==		==About this Structure==
-	~~1YTR is a~~ [[~~Single protein~~]] structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YTR OCA].	+	[[1ytr]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YTR OCA].
-		+
-	~~==Reference==~~	+
-	Structure and mode of action of the membrane-permeabilizing antimicrobial peptide pheromone plantaricin A., Kristiansen PE, Fimland G, Mantzilas D, Nissen-Meyer J, J Biol Chem. 2005 Jun 17;280(24):22945-50. Epub 2005 Apr 1. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15805109 15805109]	+
-	~~[[Category: Single protein]]~~	+
	[[Category: Fimland, G.]]		[[Category: Fimland, G.]]
	[[Category: Kristiansen, P E.]]		[[Category: Kristiansen, P E.]]
Line 33:		Line 17:
	[[Category: Micelle]]		[[Category: Micelle]]
	[[Category: Pheromone]]		[[Category: Pheromone]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 05:52:39 2008''

OCA at 02:52, 29 July 2008

OCA — Tue, 29 Jul 2008 02:52:45 GMT

←Older revision		Revision as of 02:52, 29 July 2008
Line 1:		Line 1:
-	[[Image:1ytr.~~gif~~\|left\|200px]]	+	{{Seed}}
		+	[[Image:1ytr.png\|left\|200px]]

	<!--		<!--
Line 9:		Line 10:
	{{STRUCTURE_1ytr\| PDB=1ytr \| SCENE= }}		{{STRUCTURE_1ytr\| PDB=1ytr \| SCENE= }}

-	~~'''~~NMR structure of plantaricin a in dpc micelles, 20 structures~~'''~~	+	===NMR structure of plantaricin a in dpc micelles, 20 structures===


-	~~==Overview==~~	+	<!--
-	~~The three~~-~~dimensional structure in dodecyl phosphocholine micelles of the 26~~-~~mer membrane-permeabilizing bacteriocin-like pheromone plantaricin A (PlnA) has been determined by use of nuclear magnetic resonance spectroscopy.~~ The peptide was unstructured in water but became partly structured upon exposure to micelles. An amphiphilic alpha-helix stretching from residue 12 to 21 (possibly also including residues 22 and 23) was then formed in the C-terminal part of the peptide, whereas the N-terminal part remained largely unstructured. PlnA exerted its membrane-permeabilizing antimicrobial activity through a nonchiral interaction with the target cell membrane because the d-enantiomeric form had the same activity as the natural l-form. This nonchiral interaction involved the amphiphilic alpha-helical region in the C-terminal half of PlnA because a 17-mer fragment that contains the amphiphilic alpha-helical part of the peptide had antimicrobial potency that was similar to that of the l- and d-enantiomeric forms of PlnA. Also the pheromone activity of PlnA depended on this ~~nonchiral interaction because both the l- and d-enantiomeric forms of the 17-mer fragment inhibited the pheromone activity. The pheromone activity also involved~~, ~~however~~, ~~a chiral interaction between~~ the ~~N-terminal part of PlnA and its receptor because high concentrations of~~ the ~~l-form~~ (~~but not the d-form) of a 5-mer fragment derived from the N-terminal part of PlnA had pheromone activity. The results thus reveal a novel mechanism whereby peptide pheromones such~~ as ~~PlnA may function~~. ~~An initial nonchiral interaction with membrane lipids induces alpha-helical structuring in a segment of the peptide pheromone~~. ~~The peptide becomes thereby sufficiently structured and properly positioned in the membrane interface~~, ~~thus enabling it to engage in a chiral interaction with its receptor in or near~~ the ~~membrane water interface~~. ~~This membrane~~-~~interacting mode of action explains why some peptide pheromones/hormones such as PlnA sometimes display antimicrobial activity in addition to their pheromone activity.~~	+	The line below this paragraph, {{ABSTRACT_PUBMED_15805109}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 15805109 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_15805109}}

	==About this Structure==		==About this Structure==
-	1YTR is a [[Single protein]] structure. Full ~~crystallographic~~ information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YTR OCA].	+	1YTR is a [[Single protein]] structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YTR OCA].

	==Reference==		==Reference==
Line 29:		Line 33:
	[[Category: Micelle]]		[[Category: Micelle]]
	[[Category: Pheromone]]		[[Category: Pheromone]]
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Sat May 3 16~~:46:46 2008''	+
		+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 05:52:39 2008''