1ytz - Revision history

OCA at 08:59, 14 February 2024

OCA — Wed, 14 Feb 2024 08:59:54 GMT

←Older revision		Revision as of 08:59, 14 February 2024
Line 3:		Line 3:
	<StructureSection load='1ytz' size='340' side='right'caption='[[1ytz]], [[Resolution\|resolution]] 3.00Å' scene=''>		<StructureSection load='1ytz' size='340' side='right'caption='[[1ytz]], [[Resolution\|resolution]] 3.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1ytz]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Chick Chick~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YTZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1YTZ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1ytz]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YTZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1YTZ FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=DR6:ALPHA-[4-(1,1,3,3+-+TETRAMETHYLBUTYL)PHENYL]-OMEGA-HYDROXY-POLY(OXY-1,2-ETHANEDIYL)'>DR6</scene>~~</td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 3Å</td></tr>
-	~~<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">TNNC2 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9031 CHICK])~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=DR6:ALPHA-[4-(1,1,3,3+-+TETRAMETHYLBUTYL)PHENYL]-OMEGA-HYDROXY-POLY(OXY-1,2-ETHANEDIYL)'>DR6</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ytz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ytz OCA], [https://pdbe.org/1ytz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ytz RCSB], [https://www.ebi.ac.uk/pdbsum/1ytz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ytz ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ytz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ytz OCA], [https://pdbe.org/1ytz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ytz RCSB], [https://www.ebi.ac.uk/pdbsum/1ytz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ytz ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/TNNT3_CHICK TNNT3_CHICK]] Troponin T is the tropomyosin-binding subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity. [[https://www.uniprot.org/uniprot/TNNC2_CHICK TNNC2_CHICK]] Troponin is the central regulatory protein of striated muscle contraction. Tn consists of three components: Tn-I which is the inhibitor of actomyosin ATPase, Tn-T which contains the binding site for tropomyosin and Tn-C. The binding of calcium to Tn-C abolishes the inhibitory action of Tn on actin filaments. [[https://www.uniprot.org/uniprot/TNNI2_CHICK TNNI2_CHICK]] Troponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.	+	[https://www.uniprot.org/uniprot/TNNT3_CHICK TNNT3_CHICK] Troponin T is the tropomyosin-binding subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 20:		Line 20:
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ytz ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ytz ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	Troponin senses Ca2+ to regulate contraction in striated muscle. Structures of skeletal muscle troponin composed of TnC (the sensor), TnI (the regulator), and TnT (the link to the muscle thin filament) have been determined. The structure of troponin in the Ca(2+)-activated state features a nearly twofold symmetrical assembly of TnI and TnT subunits penetrated asymmetrically by the dumbbell-shaped TnC subunit. Ca ions are thought to regulate contraction by controlling the presentation to and withdrawal of the TnI inhibitory segment from the thin filament. Here, we show that the rigid central helix of the sensor binds the inhibitory segment of TnI in the Ca(2+)-activated state. Comparison of crystal structures of troponin in the Ca(2+)-activated state at 3.0 angstroms resolution and in the Ca(2+)-free state at 7.0 angstroms resolution shows that the long framework helices of TnI and TnT, presumed to be a Ca(2+)-independent structural domain of troponin are unchanged. Loss of Ca ions causes the rigid central helix of the sensor to collapse and to release the inhibitory segment of TnI. The inhibitory segment of TnI changes conformation from an extended loop in the presence of Ca2+ to a short alpha-helix in its absence. We also show that Anapoe, a detergent molecule, increases the contractile force of muscle fibers and binds specifically, together with the TnI switch helix, in a hydrophobic pocket of TnC upon activation by Ca ions.
-
-	Ca(2+)-regulated structural changes in troponin.,Vinogradova MV, Stone DB, Malanina GG, Karatzaferi C, Cooke R, Mendelson RA, Fletterick RJ Proc Natl Acad Sci U S A. 2005 Apr 5;102(14):5038-43. Epub 2005 Mar 22. PMID:15784741<ref>PMID:15784741</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 1ytz" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
-	*[[Troponin\|Troponin]]	+	*[[Troponin 3D structures\|Troponin 3D structures]]
-	~~== References ==~~	+
-	~~<references/>~~	+
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Chick~~]]	+	[[Category: Gallus gallus]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Cooke, R]]	+	[[Category: Cooke R]]
-	[[Category: Fletterick~~, R J~~]]	+	[[Category: Fletterick RJ]]
-	[[Category: Karatzaferi, C]]	+	[[Category: Karatzaferi C]]
-	[[Category: Malanina~~, G G~~]]	+	[[Category: Malanina GG]]
-	[[Category: Mendelson~~, R A~~]]	+	[[Category: Mendelson RA]]
-	[[Category: Stone~~, D B~~]]	+	[[Category: Stone DB]]
-	[[Category: Vinogradova~~, M V]]~~	+	[[Category: Vinogradova MV]]
-	~~[[Category: Actin binding]]~~	+
-	~~[[Category: Calcium]]~~	+
-	~~[[Category: Contractile protein]]~~	+
-	~~[[Category: Muscle]]~~	+
-	~~[[Category: Thin filament]]~~	+
-	~~[[Category: Troponin~~]]	+

OCA at 16:55, 20 October 2021

OCA — Wed, 20 Oct 2021 16:55:34 GMT

←Older revision		Revision as of 16:55, 20 October 2021
Line 3:		Line 3:
	<StructureSection load='1ytz' size='340' side='right'caption='[[1ytz]], [[Resolution\|resolution]] 3.00Å' scene=''>		<StructureSection load='1ytz' size='340' side='right'caption='[[1ytz]], [[Resolution\|resolution]] 3.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1ytz]] is a 3 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Chick Chick]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YTZ OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=1YTZ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1ytz]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Chick Chick]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YTZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1YTZ FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=DR6:ALPHA-[4-(1,1,3,3+-+TETRAMETHYLBUTYL)PHENYL]-OMEGA-HYDROXY-POLY(OXY-1,2-ETHANEDIYL)'>DR6</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=DR6:ALPHA-[4-(1,1,3,3+-+TETRAMETHYLBUTYL)PHENYL]-OMEGA-HYDROXY-POLY(OXY-1,2-ETHANEDIYL)'>DR6</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">TNNC2 ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9031 CHICK])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">TNNC2 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9031 CHICK])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=1ytz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ytz OCA], [~~http~~://pdbe.org/1ytz PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1ytz RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1ytz PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1ytz ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ytz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ytz OCA], [https://pdbe.org/1ytz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ytz RCSB], [https://www.ebi.ac.uk/pdbsum/1ytz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ytz ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/TNNT3_CHICK TNNT3_CHICK]] Troponin T is the tropomyosin-binding subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity. [[~~http~~://www.uniprot.org/uniprot/TNNC2_CHICK TNNC2_CHICK]] Troponin is the central regulatory protein of striated muscle contraction. Tn consists of three components: Tn-I which is the inhibitor of actomyosin ATPase, Tn-T which contains the binding site for tropomyosin and Tn-C. The binding of calcium to Tn-C abolishes the inhibitory action of Tn on actin filaments. [[~~http~~://www.uniprot.org/uniprot/TNNI2_CHICK TNNI2_CHICK]] Troponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.	+	[[https://www.uniprot.org/uniprot/TNNT3_CHICK TNNT3_CHICK]] Troponin T is the tropomyosin-binding subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity. [[https://www.uniprot.org/uniprot/TNNC2_CHICK TNNC2_CHICK]] Troponin is the central regulatory protein of striated muscle contraction. Tn consists of three components: Tn-I which is the inhibitor of actomyosin ATPase, Tn-T which contains the binding site for tropomyosin and Tn-C. The binding of calcium to Tn-C abolishes the inhibitory action of Tn on actin filaments. [[https://www.uniprot.org/uniprot/TNNI2_CHICK TNNI2_CHICK]] Troponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 31:		Line 31:

	==See Also==		==See Also==
-	*[[Role of Troponin C in decreased muscle sensitivity to calcium\|Role of Troponin C in decreased muscle sensitivity to calcium]]
	*[[Troponin\|Troponin]]		*[[Troponin\|Troponin]]
	== References ==		== References ==

OCA at 08:52, 20 January 2021

OCA — Wed, 20 Jan 2021 08:52:40 GMT

←Older revision		Revision as of 08:52, 20 January 2021
Line 1:		Line 1:

	==Crystal structure of skeletal muscle troponin in the Ca2+-activated state==		==Crystal structure of skeletal muscle troponin in the Ca2+-activated state==
-	<StructureSection load='1ytz' size='340' side='right' caption='[[1ytz]], [[Resolution\|resolution]] 3.00Å' scene=''>	+	<StructureSection load='1ytz' size='340' side='right'caption='[[1ytz]], [[Resolution\|resolution]] 3.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1ytz]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Chick Chick]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YTZ OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1YTZ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1ytz]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Chick Chick]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YTZ OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=1YTZ FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=DR6:ALPHA-[4-(1,1,3,3+-+TETRAMETHYLBUTYL)PHENYL]-OMEGA-HYDROXY-POLY(OXY-1,2-ETHANEDIYL)'>DR6</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=DR6:ALPHA-[4-(1,1,3,3+-+TETRAMETHYLBUTYL)PHENYL]-OMEGA-HYDROXY-POLY(OXY-1,2-ETHANEDIYL)'>DR6</scene></td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">TNNC2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9031 CHICK])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">TNNC2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9031 CHICK])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1ytz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ytz OCA], [http://pdbe.org/1ytz PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1ytz RCSB], [http://www.ebi.ac.uk/pdbsum/1ytz PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1ytz ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=1ytz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ytz OCA], [http://pdbe.org/1ytz PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1ytz RCSB], [http://www.ebi.ac.uk/pdbsum/1ytz PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1ytz ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 38:		Line 38:
	</StructureSection>		</StructureSection>
	[[Category: Chick]]		[[Category: Chick]]
		+	[[Category: Large Structures]]
	[[Category: Cooke, R]]		[[Category: Cooke, R]]
	[[Category: Fletterick, R J]]		[[Category: Fletterick, R J]]

OCA at 06:51, 25 April 2018

OCA — Wed, 25 Apr 2018 06:51:58 GMT

←Older revision		Revision as of 06:51, 25 April 2018
Line 1:		Line 1:
		+
	==Crystal structure of skeletal muscle troponin in the Ca2+-activated state==		==Crystal structure of skeletal muscle troponin in the Ca2+-activated state==
	<StructureSection load='1ytz' size='340' side='right' caption='[[1ytz]], [[Resolution\|resolution]] 3.00Å' scene=''>		<StructureSection load='1ytz' size='340' side='right' caption='[[1ytz]], [[Resolution\|resolution]] 3.00Å' scene=''>
Line 5:		Line 6:
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=DR6:ALPHA-[4-(1,1,3,3+-+TETRAMETHYLBUTYL)PHENYL]-OMEGA-HYDROXY-POLY(OXY-1,2-ETHANEDIYL)'>DR6</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=DR6:ALPHA-[4-(1,1,3,3+-+TETRAMETHYLBUTYL)PHENYL]-OMEGA-HYDROXY-POLY(OXY-1,2-ETHANEDIYL)'>DR6</scene></td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">TNNC2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9031 CHICK])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">TNNC2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9031 CHICK])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ytz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ytz OCA], [http://pdbe.org/1ytz PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1ytz RCSB], [http://www.ebi.ac.uk/pdbsum/1ytz PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ytz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ytz OCA], [http://pdbe.org/1ytz PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1ytz RCSB], [http://www.ebi.ac.uk/pdbsum/1ytz PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1ytz ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 13:		Line 14:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/yt/1ytz_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/yt/1ytz_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ytz ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 45:		Line 46:
	[[Category: Vinogradova, M V]]		[[Category: Vinogradova, M V]]
	[[Category: Actin binding]]		[[Category: Actin binding]]
		+	[[Category: Calcium]]
	[[Category: Contractile protein]]		[[Category: Contractile protein]]
	[[Category: Muscle]]		[[Category: Muscle]]
	[[Category: Thin filament]]		[[Category: Thin filament]]
	[[Category: Troponin]]		[[Category: Troponin]]

OCA at 23:14, 11 September 2015

OCA — Fri, 11 Sep 2015 23:14:28 GMT

←Older revision		Revision as of 23:14, 11 September 2015
Line 2:		Line 2:
	<StructureSection load='1ytz' size='340' side='right' caption='[[1ytz]], [[Resolution\|resolution]] 3.00Å' scene=''>		<StructureSection load='1ytz' size='340' side='right' caption='[[1ytz]], [[Resolution\|resolution]] 3.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1ytz]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Gallus_gallus Gallus gallus~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YTZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1YTZ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1ytz]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Chick Chick]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YTZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1YTZ FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=DR6:ALPHA-[4-(1,1,3,3+-+TETRAMETHYLBUTYL)PHENYL]-OMEGA-HYDROXY-POLY(OXY-1,2-ETHANEDIYL)'>DR6</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=DR6:ALPHA-[4-(1,1,3,3+-+TETRAMETHYLBUTYL)PHENYL]-OMEGA-HYDROXY-POLY(OXY-1,2-ETHANEDIYL)'>DR6</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">TNNC2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9031 ~~Gallus gallus~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">TNNC2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9031 CHICK])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ytz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ytz OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1ytz RCSB], [http://www.ebi.ac.uk/pdbsum/1ytz PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ytz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ytz OCA], [http://pdbe.org/1ytz PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1ytz RCSB], [http://www.ebi.ac.uk/pdbsum/1ytz PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 27:		Line 27:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1ytz" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 35:		Line 36:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Gallus gallus~~]]	+	[[Category: Chick]]
	[[Category: Cooke, R]]		[[Category: Cooke, R]]
	[[Category: Fletterick, R J]]		[[Category: Fletterick, R J]]

OCA at 20:42, 25 December 2014

OCA — Thu, 25 Dec 2014 20:42:45 GMT

←Older revision		Revision as of 20:42, 25 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1ytz]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YTZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1YTZ FirstGlance]. <br>		<table><tr><td colspan='2'>[[1ytz]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YTZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1YTZ FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=DR6:ALPHA-[4-(1,1,3,3+-+TETRAMETHYLBUTYL)PHENYL]-OMEGA-HYDROXY-POLY(OXY-1,2-ETHANEDIYL)'>DR6</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=DR6:ALPHA-[4-(1,1,3,3+-+TETRAMETHYLBUTYL)PHENYL]-OMEGA-HYDROXY-POLY(OXY-1,2-ETHANEDIYL)'>DR6</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">TNNC2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9031 Gallus gallus])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">TNNC2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9031 Gallus gallus])</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ytz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ytz OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1ytz RCSB], [http://www.ebi.ac.uk/pdbsum/1ytz PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ytz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ytz OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1ytz RCSB], [http://www.ebi.ac.uk/pdbsum/1ytz PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/TNNT3_CHICK TNNT3_CHICK]] Troponin T is the tropomyosin-binding subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity. [[http://www.uniprot.org/uniprot/TNNC2_CHICK TNNC2_CHICK]] Troponin is the central regulatory protein of striated muscle contraction. Tn consists of three components: Tn-I which is the inhibitor of actomyosin ATPase, Tn-T which contains the binding site for tropomyosin and Tn-C. The binding of calcium to Tn-C abolishes the inhibitory action of Tn on actin filaments. [[http://www.uniprot.org/uniprot/TNNI2_CHICK TNNI2_CHICK]] Troponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 34:		Line 36:
	</StructureSection>		</StructureSection>
	[[Category: Gallus gallus]]		[[Category: Gallus gallus]]
-	[[Category: Cooke, R.]]	+	[[Category: Cooke, R]]
-	[[Category: Fletterick, R J.]]	+	[[Category: Fletterick, R J]]
-	[[Category: Karatzaferi, C.]]	+	[[Category: Karatzaferi, C]]
-	[[Category: Malanina, G G.]]	+	[[Category: Malanina, G G]]
-	[[Category: Mendelson, R A.]]	+	[[Category: Mendelson, R A]]
-	[[Category: Stone, D B.]]	+	[[Category: Stone, D B]]
-	[[Category: Vinogradova, M V.]]	+	[[Category: Vinogradova, M V]]
	[[Category: Actin binding]]		[[Category: Actin binding]]
	[[Category: Contractile protein]]		[[Category: Contractile protein]]

OCA at 20:05, 29 September 2014

OCA — Mon, 29 Sep 2014 20:05:03 GMT

←Older revision		Revision as of 20:05, 29 September 2014
Line 1:		Line 1:
-	~~{{STRUCTURE_1ytz~~\| ~~PDB~~=1ytz \| ~~SCENE~~= }}	+	==Crystal structure of skeletal muscle troponin in the Ca2+-activated state==
-	===~~Crystal~~ structure of skeletal muscle troponin in the ~~Ca2~~+-activated state~~===~~	+	<StructureSection load='1ytz' size='340' side='right' caption='[[1ytz]], [[Resolution\|resolution]] 3.00Å' scene=''>
-	~~{{ABSTRACT_PUBMED_15784741}}~~	+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1ytz]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YTZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1YTZ FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=DR6:ALPHA-[4-(1,1,3,3+-+TETRAMETHYLBUTYL)PHENYL]-OMEGA-HYDROXY-POLY(OXY-1,2-ETHANEDIYL)'>DR6</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">TNNC2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9031 Gallus gallus])</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ytz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ytz OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1ytz RCSB], [http://www.ebi.ac.uk/pdbsum/1ytz PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/yt/1ytz_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Troponin senses Ca2+ to regulate contraction in striated muscle. Structures of skeletal muscle troponin composed of TnC (the sensor), TnI (the regulator), and TnT (the link to the muscle thin filament) have been determined. The structure of troponin in the Ca(2+)-activated state features a nearly twofold symmetrical assembly of TnI and TnT subunits penetrated asymmetrically by the dumbbell-shaped TnC subunit. Ca ions are thought to regulate contraction by controlling the presentation to and withdrawal of the TnI inhibitory segment from the thin filament. Here, we show that the rigid central helix of the sensor binds the inhibitory segment of TnI in the Ca(2+)-activated state. Comparison of crystal structures of troponin in the Ca(2+)-activated state at 3.0 angstroms resolution and in the Ca(2+)-free state at 7.0 angstroms resolution shows that the long framework helices of TnI and TnT, presumed to be a Ca(2+)-independent structural domain of troponin are unchanged. Loss of Ca ions causes the rigid central helix of the sensor to collapse and to release the inhibitory segment of TnI. The inhibitory segment of TnI changes conformation from an extended loop in the presence of Ca2+ to a short alpha-helix in its absence. We also show that Anapoe, a detergent molecule, increases the contractile force of muscle fibers and binds specifically, together with the TnI switch helix, in a hydrophobic pocket of TnC upon activation by Ca ions.

-	~~==About this Structure==~~	+	Ca(2+)-regulated structural changes in troponin.,Vinogradova MV, Stone DB, Malanina GG, Karatzaferi C, Cooke R, Mendelson RA, Fletterick RJ Proc Natl Acad Sci U S A. 2005 Apr 5;102(14):5038-43. Epub 2005 Mar 22. PMID:15784741<ref>PMID:15784741</ref>
-	~~[[1ytz]] is a 3 chain structure with sequence from [http~~:~~//en~~.~~wikipedia.org/wiki/Gallus_gallus Gallus gallus]~~. ~~Full crystallographic information is available from [http~~://~~oca~~.~~weizmann~~.ac.il/~~oca-bin/ocashort?id=1YTZ OCA].~~	+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>

	==See Also==		==See Also==
	*[[Role of Troponin C in decreased muscle sensitivity to calcium\|Role of Troponin C in decreased muscle sensitivity to calcium]]		*[[Role of Troponin C in decreased muscle sensitivity to calcium\|Role of Troponin C in decreased muscle sensitivity to calcium]]
	*[[Troponin\|Troponin]]		*[[Troponin\|Troponin]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	<~~ref group="xtra">PMID:015784741<~~/~~ref~~><~~references group="xtra"~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Gallus gallus]]		[[Category: Gallus gallus]]
	[[Category: Cooke, R.]]		[[Category: Cooke, R.]]

OCA at 11:04, 11 March 2013

OCA — Mon, 11 Mar 2013 11:04:08 GMT

←Older revision		Revision as of 11:04, 11 March 2013
Line 1:		Line 1:
-	[[Image:1ytz.png\|left\|200px]]
-
	{{STRUCTURE_1ytz\| PDB=1ytz \| SCENE= }}		{{STRUCTURE_1ytz\| PDB=1ytz \| SCENE= }}
-
	===Crystal structure of skeletal muscle troponin in the Ca2+-activated state===		===Crystal structure of skeletal muscle troponin in the Ca2+-activated state===
-
	{{ABSTRACT_PUBMED_15784741}}		{{ABSTRACT_PUBMED_15784741}}

Line 11:		Line 7:

	==See Also==		==See Also==
		+	*[[Role of Troponin C in decreased muscle sensitivity to calcium\|Role of Troponin C in decreased muscle sensitivity to calcium]]
	*[[Troponin\|Troponin]]		*[[Troponin\|Troponin]]

OCA at 21:23, 20 October 2012

OCA — Sat, 20 Oct 2012 21:23:38 GMT

←Older revision		Revision as of 21:23, 20 October 2012
Line 8:		Line 8:

	==About this Structure==		==About this Structure==
-	[[1ytz]] is a 3 chain structure ~~of [[Troponin]]~~ with sequence from [http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YTZ OCA].	+	[[1ytz]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YTZ OCA].

	==See Also==		==See Also==

OCA at 13:07, 27 July 2012

OCA — Fri, 27 Jul 2012 13:07:17 GMT

←Older revision		Revision as of 13:07, 27 July 2012
Line 1:		Line 1:
	[[Image:1ytz.png\|left\|200px]]		[[Image:1ytz.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_1ytz", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_1ytz\| PDB=1ytz \| SCENE= }}		{{STRUCTURE_1ytz\| PDB=1ytz \| SCENE= }}

	===Crystal structure of skeletal muscle troponin in the Ca2+-activated state===		===Crystal structure of skeletal muscle troponin in the Ca2+-activated state===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_15784741}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 15784741 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_15784741}}		{{ABSTRACT_PUBMED_15784741}}

Line 22:		Line 11:

	==See Also==		==See Also==
-	*[[Troponin]]	+	*[[Troponin\|Troponin]]

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~15784741~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:015784741</ref><references group="xtra"/>
	[[Category: Gallus gallus]]		[[Category: Gallus gallus]]
	[[Category: Cooke, R.]]		[[Category: Cooke, R.]]
Line 35:		Line 24:
	[[Category: Vinogradova, M V.]]		[[Category: Vinogradova, M V.]]
	[[Category: Actin binding]]		[[Category: Actin binding]]
-	[[Category: Calcium]]
	[[Category: Contractile protein]]		[[Category: Contractile protein]]
	[[Category: Muscle]]		[[Category: Muscle]]
	[[Category: Thin filament]]		[[Category: Thin filament]]
	[[Category: Troponin]]		[[Category: Troponin]]