1yzx - Revision history

OCA at 13:38, 13 March 2024

OCA — Wed, 13 Mar 2024 13:38:39 GMT

←Older revision		Revision as of 13:38, 13 March 2024
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	<StructureSection load='1yzx' size='340' side='right'caption='[[1yzx]], [[Resolution\|resolution]] 1.93Å' scene=''>		<StructureSection load='1yzx' size='340' side='right'caption='[[1yzx]], [[Resolution\|resolution]] 1.93Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1yzx]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Human Human~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YZX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1YZX FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1yzx]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YZX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1YZX FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=GSF:L~~-~~GAMMA-GLUTAMYL-3-SULFINO-L-ALANYLGLYCINE'>GSF</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.93Å</td></tr>
-	<tr id='~~activity~~'><td class="sblockLbl"><b>~~Activity~~:</b></td><td class="sblockDat"><~~span class~~='~~plainlinks~~'>~~[https://en.wikipedia.org/wiki/Glutathione_transferase Glutathione transferase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.18 2.5.1.18]~~ </~~span~~></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GSF:L-GAMMA-GLUTAMYL-3-SULFINO-L-ALANYLGLYCINE'>GSF</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1yzx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1yzx OCA], [https://pdbe.org/1yzx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1yzx RCSB], [https://www.ebi.ac.uk/pdbsum/1yzx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1yzx ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1yzx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1yzx OCA], [https://pdbe.org/1yzx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1yzx RCSB], [https://www.ebi.ac.uk/pdbsum/1yzx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1yzx ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/GSTK1_HUMAN GSTK1_HUMAN]] Significant glutathione conjugating activity is found only with the model substrate, 1-chloro-2,4-dinitrobenzene (CDNB).	+	[https://www.uniprot.org/uniprot/GSTK1_HUMAN GSTK1_HUMAN] Significant glutathione conjugating activity is found only with the model substrate, 1-chloro-2,4-dinitrobenzene (CDNB).
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1yzx ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1yzx ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	Glutathione transferases (GSTs) are a superfamily of enzymes that play a vital functional role in the cellular detoxification process. They catalyze the conjugation of the thiol group of glutathione (GSH) to the electrophilic groups of a wide range of hydrophobic substrates, leading to an easier removal of the latter from the cells. The kappa class is the least studied one among various classes within the superfamily. We report here the expression, purification, and crystal structure of human kappa class GST (hGSTK), which has been determined by the multiple-isomorphous replacement method and refined to 1.93 A resolution. The overall structure of hGSTK is similar to the recently reported structure of kappa class GST from rat mitochondrion. Each subunit of the dimeric hGSTK contains a thioredoxin (TRX)-like domain and a helical domain. A molecule of glutathione sulfinate, an oxidized product of GSH, is found to bind at the G site of each monomer. One oxygen atom of the sulfino group of GSF forms a hydrogen bond with the hydroxyl group of the catalytic residue Ser16. The TRX-like domain of hGSTK shares 19% sequence identity and structure similarity with human theta class GST, suggesting that the kappa class of GST is more closely related to the theta class enzyme within the GST superfamily. The structure of the TRX-like domain of hGSTK is also similar to that of glutathione peroxidase (GPx), implying an evolutionary relationship between GST and GPx.
-
-	Thioredoxin-like domain of human kappa class glutathione transferase reveals sequence homology and structure similarity to the theta class enzyme.,Li J, Xia Z, Ding J Protein Sci. 2005 Sep;14(9):2361-9. Epub 2005 Aug 4. PMID:16081649<ref>PMID:16081649</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 1yzx" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
	*[[Glutathione S-transferase 3D structures\|Glutathione S-transferase 3D structures]]		*[[Glutathione S-transferase 3D structures\|Glutathione S-transferase 3D structures]]
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Glutathione transferase]]~~	+	[[Category: Homo sapiens]]
-	~~[[Category: Human~~]]	+
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Ding, J]]	+	[[Category: Ding J]]
-	[[Category: Li, J]]	+	[[Category: Li J]]
-	[[Category: Xia, Z]]	+	[[Category: Xia Z]]
-	~~[[Category: Glutathione sulfinate]]~~	+
-	~~[[Category: Peroxidase]]~~	+
-	~~[[Category: Transferase~~]]	+

OCA at 06:34, 6 October 2021

OCA — Wed, 06 Oct 2021 06:34:28 GMT

←Older revision		Revision as of 06:34, 6 October 2021
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	<StructureSection load='1yzx' size='340' side='right'caption='[[1yzx]], [[Resolution\|resolution]] 1.93Å' scene=''>		<StructureSection load='1yzx' size='340' side='right'caption='[[1yzx]], [[Resolution\|resolution]] 1.93Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1yzx]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YZX OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1YZX FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1yzx]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YZX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1YZX FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GSF:L-GAMMA-GLUTAMYL-3-SULFINO-L-ALANYLGLYCINE'>GSF</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GSF:L-GAMMA-GLUTAMYL-3-SULFINO-L-ALANYLGLYCINE'>GSF</scene></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Glutathione_transferase Glutathione transferase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.18 2.5.1.18] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Glutathione_transferase Glutathione transferase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.18 2.5.1.18] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1yzx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1yzx OCA], [~~http~~://pdbe.org/1yzx PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1yzx RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1yzx PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1yzx ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1yzx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1yzx OCA], [https://pdbe.org/1yzx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1yzx RCSB], [https://www.ebi.ac.uk/pdbsum/1yzx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1yzx ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/GSTK1_HUMAN GSTK1_HUMAN]] Significant glutathione conjugating activity is found only with the model substrate, 1-chloro-2,4-dinitrobenzene (CDNB).	+	[[https://www.uniprot.org/uniprot/GSTK1_HUMAN GSTK1_HUMAN]] Significant glutathione conjugating activity is found only with the model substrate, 1-chloro-2,4-dinitrobenzene (CDNB).
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 11:30, 4 December 2019

OCA — Wed, 04 Dec 2019 11:30:14 GMT

←Older revision		Revision as of 11:30, 4 December 2019
Line 1:		Line 1:

	==Crystal structure of human kappa class glutathione transferase==		==Crystal structure of human kappa class glutathione transferase==
-	<StructureSection load='1yzx' size='340' side='right' caption='[[1yzx]], [[Resolution\|resolution]] 1.93Å' scene=''>	+	<StructureSection load='1yzx' size='340' side='right'caption='[[1yzx]], [[Resolution\|resolution]] 1.93Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1yzx]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YZX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1YZX FirstGlance]. <br>		<table><tr><td colspan='2'>[[1yzx]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YZX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1YZX FirstGlance]. <br>
Line 29:		Line 29:
	</div>		</div>
	<div class="pdbe-citations 1yzx" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 1yzx" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Glutathione S-transferase 3D structures\|Glutathione S-transferase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 35:		Line 38:
	[[Category: Glutathione transferase]]		[[Category: Glutathione transferase]]
	[[Category: Human]]		[[Category: Human]]
		+	[[Category: Large Structures]]
	[[Category: Ding, J]]		[[Category: Ding, J]]
	[[Category: Li, J]]		[[Category: Li, J]]

OCA at 06:31, 20 December 2017

OCA — Wed, 20 Dec 2017 06:31:17 GMT

←Older revision		Revision as of 06:31, 20 December 2017
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	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/yz/1yzx_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/yz/1yzx_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>

OCA at 08:58, 12 October 2017

OCA — Thu, 12 Oct 2017 08:58:51 GMT

←Older revision		Revision as of 08:58, 12 October 2017
Line 1:		Line 1:
		+
	==Crystal structure of human kappa class glutathione transferase==		==Crystal structure of human kappa class glutathione transferase==
	<StructureSection load='1yzx' size='340' side='right' caption='[[1yzx]], [[Resolution\|resolution]] 1.93Å' scene=''>		<StructureSection load='1yzx' size='340' side='right' caption='[[1yzx]], [[Resolution\|resolution]] 1.93Å' scene=''>
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	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GSF:L-GAMMA-GLUTAMYL-3-SULFINO-L-ALANYLGLYCINE'>GSF</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GSF:L-GAMMA-GLUTAMYL-3-SULFINO-L-ALANYLGLYCINE'>GSF</scene></td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glutathione_transferase Glutathione transferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.18 2.5.1.18] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glutathione_transferase Glutathione transferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.18 2.5.1.18] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1yzx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1yzx OCA], [http://pdbe.org/1yzx PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1yzx RCSB], [http://www.ebi.ac.uk/pdbsum/1yzx PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1yzx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1yzx OCA], [http://pdbe.org/1yzx PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1yzx RCSB], [http://www.ebi.ac.uk/pdbsum/1yzx PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1yzx ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 17:		Line 18:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1yzx ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 28:		Line 29:
	</div>		</div>
	<div class="pdbe-citations 1yzx" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 1yzx" style="background-color:#fffaf0;"></div>
-
-	==See Also==
-	*[[Glutathione S-transferase\|Glutathione S-transferase]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 23:57, 11 September 2015

OCA — Fri, 11 Sep 2015 23:57:25 GMT

←Older revision		Revision as of 23:57, 11 September 2015
Line 2:		Line 2:
	<StructureSection load='1yzx' size='340' side='right' caption='[[1yzx]], [[Resolution\|resolution]] 1.93Å' scene=''>		<StructureSection load='1yzx' size='340' side='right' caption='[[1yzx]], [[Resolution\|resolution]] 1.93Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1yzx]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Homo_sapiens Homo sapiens~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YZX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1YZX FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1yzx]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YZX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1YZX FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GSF:L-GAMMA-GLUTAMYL-3-SULFINO-L-ALANYLGLYCINE'>GSF</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GSF:L-GAMMA-GLUTAMYL-3-SULFINO-L-ALANYLGLYCINE'>GSF</scene></td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glutathione_transferase Glutathione transferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.18 2.5.1.18] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glutathione_transferase Glutathione transferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.18 2.5.1.18] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1yzx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1yzx OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1yzx RCSB], [http://www.ebi.ac.uk/pdbsum/1yzx PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1yzx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1yzx OCA], [http://pdbe.org/1yzx PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1yzx RCSB], [http://www.ebi.ac.uk/pdbsum/1yzx PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
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	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1yzx" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 35:		Line 36:
	</StructureSection>		</StructureSection>
	[[Category: Glutathione transferase]]		[[Category: Glutathione transferase]]
-	[[Category: ~~Homo sapiens~~]]	+	[[Category: Human]]
	[[Category: Ding, J]]		[[Category: Ding, J]]
	[[Category: Li, J]]		[[Category: Li, J]]

OCA at 21:07, 25 December 2014

OCA — Thu, 25 Dec 2014 21:07:09 GMT

←Older revision		Revision as of 21:07, 25 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1yzx]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YZX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1YZX FirstGlance]. <br>		<table><tr><td colspan='2'>[[1yzx]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YZX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1YZX FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GSF:L-GAMMA-GLUTAMYL-3-SULFINO-L-ALANYLGLYCINE'>GSF</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GSF:L-GAMMA-GLUTAMYL-3-SULFINO-L-ALANYLGLYCINE'>GSF</scene></td></tr>
-	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glutathione_transferase Glutathione transferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.18 2.5.1.18] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glutathione_transferase Glutathione transferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.18 2.5.1.18] </span></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1yzx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1yzx OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1yzx RCSB], [http://www.ebi.ac.uk/pdbsum/1yzx PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1yzx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1yzx OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1yzx RCSB], [http://www.ebi.ac.uk/pdbsum/1yzx PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/GSTK1_HUMAN GSTK1_HUMAN]] Significant glutathione conjugating activity is found only with the model substrate, 1-chloro-2,4-dinitrobenzene (CDNB).
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 34:		Line 36:
	[[Category: Glutathione transferase]]		[[Category: Glutathione transferase]]
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
-	[[Category: Ding, J.]]	+	[[Category: Ding, J]]
-	[[Category: Li, J.]]	+	[[Category: Li, J]]
-	[[Category: Xia, Z.]]	+	[[Category: Xia, Z]]
	[[Category: Glutathione sulfinate]]		[[Category: Glutathione sulfinate]]
	[[Category: Peroxidase]]		[[Category: Peroxidase]]
	[[Category: Transferase]]		[[Category: Transferase]]

OCA at 20:31, 29 September 2014

OCA — Mon, 29 Sep 2014 20:31:58 GMT

←Older revision		Revision as of 20:31, 29 September 2014
Line 1:		Line 1:
-	[[~~Image~~:1yzx.~~png\|left~~\|200px]]	+	==Crystal structure of human kappa class glutathione transferase==
		+	<StructureSection load='1yzx' size='340' side='right' caption='[[1yzx]], [[Resolution\|resolution]] 1.93Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1yzx]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YZX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1YZX FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GSF:L-GAMMA-GLUTAMYL-3-SULFINO-L-ALANYLGLYCINE'>GSF</scene><br>
		+	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glutathione_transferase Glutathione transferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.18 2.5.1.18] </span></td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1yzx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1yzx OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1yzx RCSB], [http://www.ebi.ac.uk/pdbsum/1yzx PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/yz/1yzx_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Glutathione transferases (GSTs) are a superfamily of enzymes that play a vital functional role in the cellular detoxification process. They catalyze the conjugation of the thiol group of glutathione (GSH) to the electrophilic groups of a wide range of hydrophobic substrates, leading to an easier removal of the latter from the cells. The kappa class is the least studied one among various classes within the superfamily. We report here the expression, purification, and crystal structure of human kappa class GST (hGSTK), which has been determined by the multiple-isomorphous replacement method and refined to 1.93 A resolution. The overall structure of hGSTK is similar to the recently reported structure of kappa class GST from rat mitochondrion. Each subunit of the dimeric hGSTK contains a thioredoxin (TRX)-like domain and a helical domain. A molecule of glutathione sulfinate, an oxidized product of GSH, is found to bind at the G site of each monomer. One oxygen atom of the sulfino group of GSF forms a hydrogen bond with the hydroxyl group of the catalytic residue Ser16. The TRX-like domain of hGSTK shares 19% sequence identity and structure similarity with human theta class GST, suggesting that the kappa class of GST is more closely related to the theta class enzyme within the GST superfamily. The structure of the TRX-like domain of hGSTK is also similar to that of glutathione peroxidase (GPx), implying an evolutionary relationship between GST and GPx.

-	~~{{STRUCTURE_1yzx\| PDB=1yzx \| SCENE= }}~~	+	Thioredoxin-like domain of human kappa class glutathione transferase reveals sequence homology and structure similarity to the theta class enzyme.,Li J, Xia Z, Ding J Protein Sci. 2005 Sep;14(9):2361-9. Epub 2005 Aug 4. PMID:16081649<ref>PMID:16081649</ref>

-	~~===Crystal structure~~ of ~~human kappa class glutathione transferase===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_16081649}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[1yzx]] is a 2 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Homo_sapiens Homo sapiens]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=1YZX OCA].~~	+

	==See Also==		==See Also==
	*[[Glutathione S-transferase\|Glutathione S-transferase]]		*[[Glutathione S-transferase\|Glutathione S-transferase]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	<~~ref group="xtra">PMID:016081649<~~/~~ref~~><~~references group="xtra"~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Glutathione transferase]]		[[Category: Glutathione transferase]]
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]

OCA: Protected "1yzx" [edit=sysop:move=sysop]

OCA — Sat, 05 Jan 2013 17:25:47 GMT

Protected "1yzx" [edit=sysop:move=sysop]

←Older revision

Revision as of 17:25, 5 January 2013

OCA at 17:25, 5 January 2013

OCA — Sat, 05 Jan 2013 17:25:43 GMT

←Older revision		Revision as of 17:25, 5 January 2013
Line 1:		Line 1:
-	{{Seed}}
	[[Image:1yzx.png\|left\|200px]]		[[Image:1yzx.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_1yzx", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_1yzx\| PDB=1yzx \| SCENE= }}		{{STRUCTURE_1yzx\| PDB=1yzx \| SCENE= }}

	===Crystal structure of human kappa class glutathione transferase===		===Crystal structure of human kappa class glutathione transferase===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_16081649}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 16081649 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_16081649}}		{{ABSTRACT_PUBMED_16081649}}

	==About this Structure==		==About this Structure==
-	~~1YZX~~ is a 2 ~~chains~~ structure ~~of sequences~~ from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YZX OCA].	+	[[1yzx]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YZX OCA].
		+
		+	==See Also==
		+	*[[Glutathione S-transferase\|Glutathione S-transferase]]

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~16081649~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:016081649</ref><references group="xtra"/>
	[[Category: Glutathione transferase]]		[[Category: Glutathione transferase]]
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
Line 32:		Line 23:
	[[Category: Peroxidase]]		[[Category: Peroxidase]]
	[[Category: Transferase]]		[[Category: Transferase]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Feb 18 07:35:06 2009''