1z1p - Revision history

OCA at 07:03, 23 August 2023

OCA — Wed, 23 Aug 2023 07:03:05 GMT

←Older revision		Revision as of 07:03, 23 August 2023
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	<StructureSection load='1z1p' size='340' side='right'caption='[[1z1p]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='1z1p' size='340' side='right'caption='[[1z1p]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1z1p]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Aeqvi Aeqvi~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Z1P OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1Z1P FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1z1p]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Aequorea_victoria Aequorea victoria]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Z1P OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1Z1P FirstGlance]. <br>
-	</td></tr><tr id='~~NonStdRes~~'><td class="sblockLbl"><b>[[~~Non-Standard_Residue~~\|~~NonStd Res~~:]]</b></td><td class="sblockDat">~~<scene name='pdbligand=CR0:[2~~-~~(1-AMINO-2-HYDROXYPROPYL)-2-HYDROXY-4-ISOBUTYL-5-OXO-2~~,~~5-DIHYDRO-1H-IMIDAZOL-1-YL~~]~~ACETALDEHYDE'>CR0</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2Å</td></tr>
-	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"><~~div style~~='~~overflow~~: ~~auto; max~~-~~height: 3em;~~'>~~[[1s6z\|1s6z]]~~</~~div~~></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CR0:[2-(1-AMINO-2-HYDROXYPROPYL)-2-HYDROXY-4-ISOBUTYL-5-OXO-2,5-DIHYDRO-1H-IMIDAZOL-1-YL]ACETALDEHYDE'>CR0</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1z1p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1z1p OCA], [https://pdbe.org/1z1p PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1z1p RCSB], [https://www.ebi.ac.uk/pdbsum/1z1p PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1z1p ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1z1p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1z1p OCA], [https://pdbe.org/1z1p PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1z1p RCSB], [https://www.ebi.ac.uk/pdbsum/1z1p PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1z1p ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/GFP_AEQVI GFP_AEQVI] Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca(2+)-activated photoprotein aequorin.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Aeqvi~~]]	+	[[Category: Aequorea victoria]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Patel~~, H N~~]]	+	[[Category: Patel HN]]
-	[[Category: Rosenow~~, M A~~]]	+	[[Category: Rosenow MA]]
-	[[Category: Wachter~~, R M]]~~	+	[[Category: Wachter RM]]
-	~~[[Category: Beta barrel]]~~	+
-	~~[[Category: Gfp]]~~	+
-	~~[[Category: Luminescent protein]]~~	+
-	~~[[Category: Uv/vis absorbing yellow chromophore~~]]	+

OCA at 13:35, 13 October 2021

OCA — Wed, 13 Oct 2021 13:35:59 GMT

←Older revision		Revision as of 13:35, 13 October 2021
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	<StructureSection load='1z1p' size='340' side='right'caption='[[1z1p]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='1z1p' size='340' side='right'caption='[[1z1p]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1z1p]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Aeqvi Aeqvi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Z1P OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1Z1P FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1z1p]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Aeqvi Aeqvi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Z1P OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1Z1P FirstGlance]. <br>
	</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CR0:[2-(1-AMINO-2-HYDROXYPROPYL)-2-HYDROXY-4-ISOBUTYL-5-OXO-2,5-DIHYDRO-1H-IMIDAZOL-1-YL]ACETALDEHYDE'>CR0</scene></td></tr>		</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CR0:[2-(1-AMINO-2-HYDROXYPROPYL)-2-HYDROXY-4-ISOBUTYL-5-OXO-2,5-DIHYDRO-1H-IMIDAZOL-1-YL]ACETALDEHYDE'>CR0</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1s6z\|1s6z]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1s6z\|1s6z]]</div></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1z1p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1z1p OCA], [~~http~~://pdbe.org/1z1p PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1z1p RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1z1p PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1z1p ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1z1p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1z1p OCA], [https://pdbe.org/1z1p PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1z1p RCSB], [https://www.ebi.ac.uk/pdbsum/1z1p PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1z1p ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==

OCA at 10:03, 8 January 2020

OCA — Wed, 08 Jan 2020 10:03:17 GMT

←Older revision		Revision as of 10:03, 8 January 2020
Line 1:		Line 1:

	==Y66L variant of Enhanced Green Fluorescent Protein with 412-nm Absorbing Chromophore==		==Y66L variant of Enhanced Green Fluorescent Protein with 412-nm Absorbing Chromophore==
-	<StructureSection load='1z1p' size='340' side='right' caption='[[1z1p]], [[Resolution\|resolution]] 2.00Å' scene=''>	+	<StructureSection load='1z1p' size='340' side='right'caption='[[1z1p]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1z1p]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Aeqvi Aeqvi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Z1P OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1Z1P FirstGlance]. <br>		<table><tr><td colspan='2'>[[1z1p]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Aeqvi Aeqvi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Z1P OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1Z1P FirstGlance]. <br>
Line 29:		Line 29:

	==See Also==		==See Also==
-	*[[Green Fluorescent Protein\|Green Fluorescent Protein]]	+	*[[Green Fluorescent Protein 3D structures\|Green Fluorescent Protein 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 35:		Line 35:
	</StructureSection>		</StructureSection>
	[[Category: Aeqvi]]		[[Category: Aeqvi]]
		+	[[Category: Large Structures]]
	[[Category: Patel, H N]]		[[Category: Patel, H N]]
	[[Category: Rosenow, M A]]		[[Category: Rosenow, M A]]

OCA at 06:23, 4 April 2018

OCA — Wed, 04 Apr 2018 06:23:54 GMT

←Older revision		Revision as of 06:23, 4 April 2018
Line 1:		Line 1:
		+
	==Y66L variant of Enhanced Green Fluorescent Protein with 412-nm Absorbing Chromophore==		==Y66L variant of Enhanced Green Fluorescent Protein with 412-nm Absorbing Chromophore==
	<StructureSection load='1z1p' size='340' side='right' caption='[[1z1p]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='1z1p' size='340' side='right' caption='[[1z1p]], [[Resolution\|resolution]] 2.00Å' scene=''>
Line 5:		Line 6:
	</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CR0:[2-(1-AMINO-2-HYDROXYPROPYL)-2-HYDROXY-4-ISOBUTYL-5-OXO-2,5-DIHYDRO-1H-IMIDAZOL-1-YL]ACETALDEHYDE'>CR0</scene></td></tr>		</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CR0:[2-(1-AMINO-2-HYDROXYPROPYL)-2-HYDROXY-4-ISOBUTYL-5-OXO-2,5-DIHYDRO-1H-IMIDAZOL-1-YL]ACETALDEHYDE'>CR0</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1s6z\|1s6z]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1s6z\|1s6z]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1z1p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1z1p OCA], [http://pdbe.org/1z1p PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1z1p RCSB], [http://www.ebi.ac.uk/pdbsum/1z1p PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1z1p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1z1p OCA], [http://pdbe.org/1z1p PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1z1p RCSB], [http://www.ebi.ac.uk/pdbsum/1z1p PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1z1p ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 11:		Line 12:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/z1/1z1p_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/z1/1z1p_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1z1p ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 13:28, 11 September 2015

OCA — Fri, 11 Sep 2015 13:28:39 GMT

←Older revision		Revision as of 13:28, 11 September 2015
Line 2:		Line 2:
	<StructureSection load='1z1p' size='340' side='right' caption='[[1z1p]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='1z1p' size='340' side='right' caption='[[1z1p]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1z1p]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Aequorea_victoria Aequorea victoria~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Z1P OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1Z1P FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1z1p]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Aeqvi Aeqvi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Z1P OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1Z1P FirstGlance]. <br>
	</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CR0:[2-(1-AMINO-2-HYDROXYPROPYL)-2-HYDROXY-4-ISOBUTYL-5-OXO-2,5-DIHYDRO-1H-IMIDAZOL-1-YL]ACETALDEHYDE'>CR0</scene></td></tr>		</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CR0:[2-(1-AMINO-2-HYDROXYPROPYL)-2-HYDROXY-4-ISOBUTYL-5-OXO-2,5-DIHYDRO-1H-IMIDAZOL-1-YL]ACETALDEHYDE'>CR0</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1s6z\|1s6z]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1s6z\|1s6z]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1z1p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1z1p OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1z1p RCSB], [http://www.ebi.ac.uk/pdbsum/1z1p PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1z1p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1z1p OCA], [http://pdbe.org/1z1p PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1z1p RCSB], [http://www.ebi.ac.uk/pdbsum/1z1p PDBsum]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 25:		Line 25:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1z1p" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 32:		Line 33:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Aequorea victoria~~]]	+	[[Category: Aeqvi]]
	[[Category: Patel, H N]]		[[Category: Patel, H N]]
	[[Category: Rosenow, M A]]		[[Category: Rosenow, M A]]

OCA at 08:50, 8 January 2015

OCA — Thu, 08 Jan 2015 08:50:51 GMT

←Older revision		Revision as of 08:50, 8 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1z1p]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Aequorea_victoria Aequorea victoria]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Z1P OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1Z1P FirstGlance]. <br>		<table><tr><td colspan='2'>[[1z1p]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Aequorea_victoria Aequorea victoria]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Z1P OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1Z1P FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CR0:[2-(1-AMINO-2-HYDROXYPROPYL)-2-HYDROXY-4-ISOBUTYL-5-OXO-2,5-DIHYDRO-1H-IMIDAZOL-1-YL]ACETALDEHYDE'>CR0</scene></td></tr>	+	</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CR0:[2-(1-AMINO-2-HYDROXYPROPYL)-2-HYDROXY-4-ISOBUTYL-5-OXO-2,5-DIHYDRO-1H-IMIDAZOL-1-YL]ACETALDEHYDE'>CR0</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1s6z\|1s6z]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1s6z\|1s6z]]</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1z1p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1z1p OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1z1p RCSB], [http://www.ebi.ac.uk/pdbsum/1z1p PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1z1p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1z1p OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1z1p RCSB], [http://www.ebi.ac.uk/pdbsum/1z1p PDBsum]</span></td></tr>
-	<table>	+	</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 33:		Line 33:
	</StructureSection>		</StructureSection>
	[[Category: Aequorea victoria]]		[[Category: Aequorea victoria]]
-	[[Category: Patel, H N.]]	+	[[Category: Patel, H N]]
-	[[Category: Rosenow, M A.]]	+	[[Category: Rosenow, M A]]
-	[[Category: Wachter, R M.]]	+	[[Category: Wachter, R M]]
	[[Category: Beta barrel]]		[[Category: Beta barrel]]
	[[Category: Gfp]]		[[Category: Gfp]]
	[[Category: Luminescent protein]]		[[Category: Luminescent protein]]
	[[Category: Uv/vis absorbing yellow chromophore]]		[[Category: Uv/vis absorbing yellow chromophore]]

OCA at 05:12, 3 October 2014

OCA — Fri, 03 Oct 2014 05:12:19 GMT

←Older revision		Revision as of 05:12, 3 October 2014
Line 1:		Line 1:
-	[[~~Image:~~1z1p.~~png~~\|~~left~~\|200px]]	+	==Y66L variant of Enhanced Green Fluorescent Protein with 412-nm Absorbing Chromophore==
		+	<StructureSection load='1z1p' size='340' side='right' caption='[[1z1p]], [[Resolution\|resolution]] 2.00Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1z1p]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Aequorea_victoria Aequorea victoria]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Z1P OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1Z1P FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CR0:[2-(1-AMINO-2-HYDROXYPROPYL)-2-HYDROXY-4-ISOBUTYL-5-OXO-2,5-DIHYDRO-1H-IMIDAZOL-1-YL]ACETALDEHYDE'>CR0</scene></td></tr>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1s6z\|1s6z]]</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1z1p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1z1p OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1z1p RCSB], [http://www.ebi.ac.uk/pdbsum/1z1p PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/z1/1z1p_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The mechanism of chromophore biosynthesis in green fluorescent protein (GFP) is triggered by a spontaneous main chain cyclization reaction of residues 65-67. Here, we demonstrate that the initially colorless Y66L variant, designed to trap chromophore precursor states, is oxidatively modified to generate yellow chromophores that absorb at 412 and 374 nm. High- and low-pH crystal structures determined to 2.0 and 1.5 A resolution, respectively, are consistent with pi-orbital conjugation of a planar Leu66-derived adduct with the imidazolinone ring, which is approximately 90 and 100% dehydrated, respectively. Time-, base-, and oxygen-dependent optical properties suggest that the yellow chromophores are generated from a 338 nm-absorbing intermediate, interpreted to be the Y66L analogue of the wild-type GFP chromophore. Generation of this species is catalyzed by a general base such as formate, and proceeds via a cyclization-oxidation-dehydration mechanism. The data suggest that a hydration-dehydration equilibrium exists in the cyclic form of the peptide, and that dehydration is favored upon extensive conjugation with the modified side chain. We conclude that the mechanism of GFP chromophore biosynthesis is not driven by the aromatic character of residue 66. In the low-pH X-ray structure, a highly unusual cross-link is observed between His148 and the oxidized Leu66 side chain, suggesting a conjugate addition reaction of the imidazole nitrogen to the highly electrophilic diene group of the yellow chromophore. The reactivity described here further expands the chemical diversity observed in the active site of GFP-like proteins, and may allow for covalent attachment of functional groups to the protein scaffold for catalytic purposes.

-	~~{{STRUCTURE_1z1p\| PDB=1z1p \| SCENE= }}~~	+	Oxidative chemistry in the GFP active site leads to covalent cross-linking of a modified leucine side chain with a histidine imidazole: implications for the mechanism of chromophore formation.,Rosenow MA, Patel HN, Wachter RM Biochemistry. 2005 Jun 14;44(23):8303-11. PMID:15938620<ref>PMID:15938620</ref>

-	~~===Y66L variant~~ of ~~Enhanced Green Fluorescent Protein with 412-nm Absorbing Chromophore===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_15938620}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[1z1p]] is a 1 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Aequorea_victoria Aequorea victoria]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=1Z1P OCA].~~	+

	==See Also==		==See Also==
-	*[[Alyssa Marsico/Sandbox 1\|Alyssa Marsico/Sandbox 1]]
-	*[[Devon McCarthy/Sandbox 1\|Devon McCarthy/Sandbox 1]]
	*[[Green Fluorescent Protein\|Green Fluorescent Protein]]		*[[Green Fluorescent Protein\|Green Fluorescent Protein]]
-	*[[Sandbox104\|Sandbox104]]	+	== References ==
-	*[[User:Joanne Lau/Sandbox 5\|User:Joanne Lau/Sandbox 5]]	+	<references/>
-		+	__TOC__
-	==~~Reference~~==	+	</StructureSection>
-	<~~ref group="xtra">PMID:015938620<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Aequorea victoria]]		[[Category: Aequorea victoria]]
	[[Category: Patel, H N.]]		[[Category: Patel, H N.]]

OCA at 19:14, 20 October 2012

OCA — Sat, 20 Oct 2012 19:14:27 GMT

←Older revision		Revision as of 19:14, 20 October 2012
Line 8:		Line 8:

	==About this Structure==		==About this Structure==
-	[[1z1p]] is a 1 chain structure ~~of [[Alyssa Marsico/Sandbox 1]], [[Devon McCarthy/Sandbox 1]], [[Green Fluorescent Protein]], [[Sandbox104]] and [[User:Joanne Lau/Sandbox 5]]~~ with sequence from [http://en.wikipedia.org/wiki/Aequorea_victoria Aequorea victoria]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Z1P OCA].	+	[[1z1p]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Aequorea_victoria Aequorea victoria]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Z1P OCA].

	==See Also==		==See Also==

OCA: Protected "1z1p" [edit=sysop:move=sysop]

OCA — Fri, 27 Jul 2012 06:43:08 GMT

Protected "1z1p" [edit=sysop:move=sysop]

←Older revision

Revision as of 06:43, 27 July 2012

OCA at 06:43, 27 July 2012

OCA — Fri, 27 Jul 2012 06:43:07 GMT

←Older revision		Revision as of 06:43, 27 July 2012
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-	{{Seed}}
	[[Image:1z1p.png\|left\|200px]]		[[Image:1z1p.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_1z1p", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
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-	or leave the SCENE parameter empty for the default display.
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	{{STRUCTURE_1z1p\| PDB=1z1p \| SCENE= }}		{{STRUCTURE_1z1p\| PDB=1z1p \| SCENE= }}

	===Y66L variant of Enhanced Green Fluorescent Protein with 412-nm Absorbing Chromophore===		===Y66L variant of Enhanced Green Fluorescent Protein with 412-nm Absorbing Chromophore===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_15938620}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 15938620 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_15938620}}		{{ABSTRACT_PUBMED_15938620}}

	==About this Structure==		==About this Structure==
-	~~1Z1P~~ is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Aequorea_victoria Aequorea victoria]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Z1P OCA].	+	[[1z1p]] is a 1 chain structure of [[Alyssa Marsico/Sandbox 1]], [[Devon McCarthy/Sandbox 1]], [[Green Fluorescent Protein]], [[Sandbox104]] and [[User:Joanne Lau/Sandbox 5]] with sequence from [http://en.wikipedia.org/wiki/Aequorea_victoria Aequorea victoria]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Z1P OCA].
		+
		+	==See Also==
		+	*[[Alyssa Marsico/Sandbox 1\|Alyssa Marsico/Sandbox 1]]
		+	*[[Devon McCarthy/Sandbox 1\|Devon McCarthy/Sandbox 1]]
		+	*[[Green Fluorescent Protein\|Green Fluorescent Protein]]
		+	*[[Sandbox104\|Sandbox104]]
		+	*[[User:Joanne Lau/Sandbox 5\|User:Joanne Lau/Sandbox 5]]

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~15938620~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:015938620</ref><references group="xtra"/>
	[[Category: Aequorea victoria]]		[[Category: Aequorea victoria]]
	[[Category: Patel, H N.]]		[[Category: Patel, H N.]]
Line 32:		Line 27:
	[[Category: Luminescent protein]]		[[Category: Luminescent protein]]
	[[Category: Uv/vis absorbing yellow chromophore]]		[[Category: Uv/vis absorbing yellow chromophore]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jul 1 09:47:10 2009''