1z7i - Revision history

OCA at 09:02, 14 February 2024

OCA — Wed, 14 Feb 2024 09:02:40 GMT

←Older revision		Revision as of 09:02, 14 February 2024
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	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1z7i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1z7i OCA], [https://pdbe.org/1z7i PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1z7i RCSB], [https://www.ebi.ac.uk/pdbsum/1z7i PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1z7i ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1z7i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1z7i OCA], [https://pdbe.org/1z7i PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1z7i RCSB], [https://www.ebi.ac.uk/pdbsum/1z7i PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1z7i ProSAT]</span></td></tr>
	</table>		</table>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	The high-resolution structure of the DNA (5'-GTGTACA-C-3') with the selenium derivatization at the 2'-position of T2 was determined via MAD and SAD phasing. The selenium-derivatized structure (1.28 A resolution) with the 2'-Se modification in the minor groove is isomorphorous to the native structure (2.0 A). To directly compare with the conventional bromine derivatization, we incorporated bromine into the 5-postion of T4, determined the bromine-derivatized DNA structure at 1.5 A resolution, and found that the local backbone torsion angles and solvent hydration patterns were altered in the structure with the Br incorporation in the major groove. Furthermore, while the native and Br-derivatized DNAs needed over a week to form reasonable-size crystals, we observed that the Se-derivatized DNAs grew crystals overnight with high-diffraction quality, suggesting that the Se derivatization facilitated the crystal formation. In addition, the Se-derivatized DNA sequences crystallized under a broader range of buffer conditions, and generally had a faster crystal growth rate. Our experimental results indicate that the selenium derivatization of DNAs may facilitate the determination of nucleic acid X-ray crystal structures in phasing and high-quality crystal growth. In addition, our results suggest that the Se derivatization can be an alternative to the conventional Br derivatization.
-
-	Selenium derivatization of nucleic acids for crystallography.,Jiang J, Sheng J, Carrasco N, Huang Z Nucleic Acids Res. 2007;35(2):477-85. Epub 2006 Dec 14. PMID:17169989<ref>PMID:17169989</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 1z7i" style="background-color:#fffaf0;"></div>
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 10:30, 16 August 2023

OCA — Wed, 16 Aug 2023 10:30:29 GMT

←Older revision		Revision as of 10:30, 16 August 2023
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1z7i]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Z7I OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1Z7I FirstGlance]. <br>		<table><tr><td colspan='2'>[[1z7i]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Z7I OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1Z7I FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=SPM:SPERMINE'>SPM</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.28Å</td></tr>
-	<tr id='~~NonStdRes~~'><td class="sblockLbl"><b>[[~~Non-Standard_Residue~~\|~~NonStd Res~~:]]</b></td><td class="sblockDat"><scene name='pdbligand=UMS:2-METHYLSELENYL-2-DEOXYURIDINE-5-PHOSPHATE'>UMS</scene~~></td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SPM:SPERMINE'>SPM</scene>, <scene name='pdbligand=UMS:2-METHYLSELENYL-2-DEOXYURIDINE-5-PHOSPHATE'>UMS</scene></td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1dns\|1dns]], [[1d78\|1d78]], [[1d79\|1d79]]</div~~></td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1z7i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1z7i OCA], [https://pdbe.org/1z7i PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1z7i RCSB], [https://www.ebi.ac.uk/pdbsum/1z7i PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1z7i ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1z7i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1z7i OCA], [https://pdbe.org/1z7i PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1z7i RCSB], [https://www.ebi.ac.uk/pdbsum/1z7i PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1z7i ProSAT]</span></td></tr>
	</table>		</table>
Line 23:		Line 22:
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Carrasco, N]]	+	[[Category: Carrasco N]]
-	[[Category: Huang, Z]]	+	[[Category: Huang Z]]
-	[[Category: Jiang, J]]	+	[[Category: Jiang J]]
-	~~[[Category: 2'-methylseleno-uridine]]~~	+
-	~~[[Category: Dna]]~~	+
-	~~[[Category: Se-dna]]~~	+
-	~~[[Category: Selenium derivatization~~]]	+

OCA at 08:18, 10 November 2021

OCA — Wed, 10 Nov 2021 08:18:13 GMT

←Older revision		Revision as of 08:18, 10 November 2021
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	<StructureSection load='1z7i' size='340' side='right'caption='[[1z7i]], [[Resolution\|resolution]] 1.28Å' scene=''>		<StructureSection load='1z7i' size='340' side='right'caption='[[1z7i]], [[Resolution\|resolution]] 1.28Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1z7i]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Z7I OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1Z7I FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1z7i]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Z7I OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1Z7I FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SPM:SPERMINE'>SPM</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SPM:SPERMINE'>SPM</scene></td></tr>
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=UMS:2-METHYLSELENYL-2-DEOXYURIDINE-5-PHOSPHATE'>UMS</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=UMS:2-METHYLSELENYL-2-DEOXYURIDINE-5-PHOSPHATE'>UMS</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1dns\|1dns]], [[1d78\|1d78]], [[1d79\|1d79]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1dns\|1dns]], [[1d78\|1d78]], [[1d79\|1d79]]</div></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1z7i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1z7i OCA], [~~http~~://pdbe.org/1z7i PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1z7i RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1z7i PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1z7i ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1z7i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1z7i OCA], [https://pdbe.org/1z7i PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1z7i RCSB], [https://www.ebi.ac.uk/pdbsum/1z7i PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1z7i ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 09:35, 5 February 2020

OCA — Wed, 05 Feb 2020 09:35:50 GMT

←Older revision		Revision as of 09:35, 5 February 2020
Line 1:		Line 1:

	==2'-Me-Se Derivitation of A-DNA Octamer G(UMSe)GTACAC==		==2'-Me-Se Derivitation of A-DNA Octamer G(UMSe)GTACAC==
-	<StructureSection load='1z7i' size='340' side='right' caption='[[1z7i]], [[Resolution\|resolution]] 1.28Å' scene=''>	+	<StructureSection load='1z7i' size='340' side='right'caption='[[1z7i]], [[Resolution\|resolution]] 1.28Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1z7i]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Z7I OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1Z7I FirstGlance]. <br>		<table><tr><td colspan='2'>[[1z7i]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Z7I OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1Z7I FirstGlance]. <br>
Line 22:		Line 22:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Carrasco, N]]		[[Category: Carrasco, N]]
	[[Category: Huang, Z]]		[[Category: Huang, Z]]

OCA at 09:01, 2 May 2018

OCA — Wed, 02 May 2018 09:01:14 GMT

←Older revision		Revision as of 09:01, 2 May 2018
Line 1:		Line 1:
		+
	==2'-Me-Se Derivitation of A-DNA Octamer G(UMSe)GTACAC==		==2'-Me-Se Derivitation of A-DNA Octamer G(UMSe)GTACAC==
	<StructureSection load='1z7i' size='340' side='right' caption='[[1z7i]], [[Resolution\|resolution]] 1.28Å' scene=''>		<StructureSection load='1z7i' size='340' side='right' caption='[[1z7i]], [[Resolution\|resolution]] 1.28Å' scene=''>
Line 6:		Line 7:
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=UMS:2-METHYLSELENYL-2-DEOXYURIDINE-5-PHOSPHATE'>UMS</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=UMS:2-METHYLSELENYL-2-DEOXYURIDINE-5-PHOSPHATE'>UMS</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1dns\|1dns]], [[1d78\|1d78]], [[1d79\|1d79]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1dns\|1dns]], [[1d78\|1d78]], [[1d79\|1d79]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1z7i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1z7i OCA], [http://pdbe.org/1z7i PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1z7i RCSB], [http://www.ebi.ac.uk/pdbsum/1z7i PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1z7i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1z7i OCA], [http://pdbe.org/1z7i PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1z7i RCSB], [http://www.ebi.ac.uk/pdbsum/1z7i PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1z7i ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 08:34, 10 September 2015

OCA — Thu, 10 Sep 2015 08:34:07 GMT

←Older revision		Revision as of 08:34, 10 September 2015
Line 6:		Line 6:
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=UMS:2-METHYLSELENYL-2-DEOXYURIDINE-5-PHOSPHATE'>UMS</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=UMS:2-METHYLSELENYL-2-DEOXYURIDINE-5-PHOSPHATE'>UMS</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1dns\|1dns]], [[1d78\|1d78]], [[1d79\|1d79]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1dns\|1dns]], [[1d78\|1d78]], [[1d79\|1d79]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1z7i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1z7i OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1z7i RCSB], [http://www.ebi.ac.uk/pdbsum/1z7i PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1z7i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1z7i OCA], [http://pdbe.org/1z7i PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1z7i RCSB], [http://www.ebi.ac.uk/pdbsum/1z7i PDBsum]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 16:		Line 16:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1z7i" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 09:16, 8 January 2015

OCA — Thu, 08 Jan 2015 09:16:53 GMT

←Older revision		Revision as of 09:16, 8 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1z7i]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Z7I OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1Z7I FirstGlance]. <br>		<table><tr><td colspan='2'>[[1z7i]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Z7I OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1Z7I FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SPM:SPERMINE'>SPM</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SPM:SPERMINE'>SPM</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=UMS:2-METHYLSELENYL-2-DEOXYURIDINE-5-PHOSPHATE'>UMS</scene></td></tr>	+	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=UMS:2-METHYLSELENYL-2-DEOXYURIDINE-5-PHOSPHATE'>UMS</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1dns\|1dns]], [[1d78\|1d78]], [[1d79\|1d79]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1dns\|1dns]], [[1d78\|1d78]], [[1d79\|1d79]]</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1z7i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1z7i OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1z7i RCSB], [http://www.ebi.ac.uk/pdbsum/1z7i PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1z7i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1z7i OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1z7i RCSB], [http://www.ebi.ac.uk/pdbsum/1z7i PDBsum]</span></td></tr>
-	<table>	+	</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 20:		Line 20:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Carrasco, N.]]	+	[[Category: Carrasco, N]]
-	[[Category: Huang, Z.]]	+	[[Category: Huang, Z]]
-	[[Category: Jiang, J.]]	+	[[Category: Jiang, J]]
	[[Category: 2'-methylseleno-uridine]]		[[Category: 2'-methylseleno-uridine]]
	[[Category: Dna]]		[[Category: Dna]]
	[[Category: Se-dna]]		[[Category: Se-dna]]
	[[Category: Selenium derivatization]]		[[Category: Selenium derivatization]]

OCA at 23:21, 28 September 2014

OCA — Sun, 28 Sep 2014 23:21:18 GMT

←Older revision		Revision as of 23:21, 28 September 2014
Line 1:		Line 1:
-	[[~~Image:~~1z7i.~~png~~\|~~left~~\|~~200px~~]]	+	==2'-Me-Se Derivitation of A-DNA Octamer G(UMSe)GTACAC==
		+	<StructureSection load='1z7i' size='340' side='right' caption='[[1z7i]], [[Resolution\|resolution]] 1.28Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1z7i]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Z7I OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1Z7I FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SPM:SPERMINE'>SPM</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=UMS:2-METHYLSELENYL-2-DEOXYURIDINE-5-PHOSPHATE'>UMS</scene></td></tr>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1dns\|1dns]], [[1d78\|1d78]], [[1d79\|1d79]]</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1z7i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1z7i OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1z7i RCSB], [http://www.ebi.ac.uk/pdbsum/1z7i PDBsum]</span></td></tr>
		+	<table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The high-resolution structure of the DNA (5'-GTGTACA-C-3') with the selenium derivatization at the 2'-position of T2 was determined via MAD and SAD phasing. The selenium-derivatized structure (1.28 A resolution) with the 2'-Se modification in the minor groove is isomorphorous to the native structure (2.0 A). To directly compare with the conventional bromine derivatization, we incorporated bromine into the 5-postion of T4, determined the bromine-derivatized DNA structure at 1.5 A resolution, and found that the local backbone torsion angles and solvent hydration patterns were altered in the structure with the Br incorporation in the major groove. Furthermore, while the native and Br-derivatized DNAs needed over a week to form reasonable-size crystals, we observed that the Se-derivatized DNAs grew crystals overnight with high-diffraction quality, suggesting that the Se derivatization facilitated the crystal formation. In addition, the Se-derivatized DNA sequences crystallized under a broader range of buffer conditions, and generally had a faster crystal growth rate. Our experimental results indicate that the selenium derivatization of DNAs may facilitate the determination of nucleic acid X-ray crystal structures in phasing and high-quality crystal growth. In addition, our results suggest that the Se derivatization can be an alternative to the conventional Br derivatization.

-	~~{{STRUCTURE_1z7i\| PDB=1z7i \| SCENE= }}~~	+	Selenium derivatization of nucleic acids for crystallography.,Jiang J, Sheng J, Carrasco N, Huang Z Nucleic Acids Res. 2007;35(2):477-85. Epub 2006 Dec 14. PMID:17169989<ref>PMID:17169989</ref>

-	~~===2'-Me-Se Derivitation~~ of ~~A-DNA Octamer G(UMSe)GTACAC===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_17169989}}~~	+	== References ==
-		+	<references/>
-	~~==About this Structure==~~	+	__TOC__
-	~~[[1z7i]] is a 1 chain structure~~. ~~Full crystallographic information is available from [http://oca~~.~~weizmann~~.~~ac.il~~/~~oca-bin/ocashort?id=1Z7I OCA].~~	+	</StructureSection>
-		+
-	==~~See Also==~~	+
-	*[[Transthyretin\|Transthyretin]]	+
-		+
-	~~==Reference~~==	+
-	<~~ref group="xtra">PMID:017169989<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Carrasco, N.]]		[[Category: Carrasco, N.]]
	[[Category: Huang, Z.]]		[[Category: Huang, Z.]]

OCA at 09:09, 20 October 2012

OCA — Sat, 20 Oct 2012 09:09:17 GMT

←Older revision		Revision as of 09:09, 20 October 2012
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	==About this Structure==		==About this Structure==
-	[[1z7i]] is a 1 chain structure ~~of [[Transthyretin]]~~. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Z7I OCA].	+	[[1z7i]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Z7I OCA].

	==See Also==		==See Also==

OCA: Protected "1z7i" [edit=sysop:move=sysop]

OCA — Wed, 25 Jul 2012 21:54:48 GMT

Protected "1z7i" [edit=sysop:move=sysop]

←Older revision

Revision as of 21:54, 25 July 2012