1zb1 - Revision history

OCA at 09:03, 14 February 2024

OCA — Wed, 14 Feb 2024 09:03:18 GMT

←Older revision		Revision as of 09:03, 14 February 2024
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	<StructureSection load='1zb1' size='340' side='right'caption='[[1zb1]], [[Resolution\|resolution]] 1.95Å' scene=''>		<StructureSection load='1zb1' size='340' side='right'caption='[[1zb1]], [[Resolution\|resolution]] 1.95Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1zb1]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Atcc_18824 Atcc 18824~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZB1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1ZB1 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1zb1]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZB1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1ZB1 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1zb1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1zb1 OCA], [https://pdbe.org/1zb1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1zb1 RCSB], [https://www.ebi.ac.uk/pdbsum/1zb1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1zb1 ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.95Å</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1zb1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1zb1 OCA], [https://pdbe.org/1zb1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1zb1 RCSB], [https://www.ebi.ac.uk/pdbsum/1zb1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1zb1 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/BRO1_YEAST BRO1_YEAST]] Class E VPS protein involved in concentration and sorting of cargo proteins of the multivesicular body (MVB) for incorporation into intralumenal vesicles. Fusion between endosomes and the vacuole will then target the cargo proteins to the vacuolar lumen. Acts as an adapter that recruits the DOA4 deubiquitinase to the endosomes, leading to deubiquitination of cargo proteins prior to the lumenal sequestration. Its association to the endosomes depends on SNF7 and its dissociation requires VPS4. Interacts functionally with the Pkc1p-mitogen-activated protein kinase pathway.<ref>PMID:8649366</ref> <ref>PMID:11454748</ref> <ref>PMID:12062418</ref> <ref>PMID:12668726</ref> <ref>PMID:14523026</ref> <ref>PMID:15326198</ref>	+	[https://www.uniprot.org/uniprot/BRO1_YEAST BRO1_YEAST] Class E VPS protein involved in concentration and sorting of cargo proteins of the multivesicular body (MVB) for incorporation into intralumenal vesicles. Fusion between endosomes and the vacuole will then target the cargo proteins to the vacuolar lumen. Acts as an adapter that recruits the DOA4 deubiquitinase to the endosomes, leading to deubiquitination of cargo proteins prior to the lumenal sequestration. Its association to the endosomes depends on SNF7 and its dissociation requires VPS4. Interacts functionally with the Pkc1p-mitogen-activated protein kinase pathway.<ref>PMID:8649366</ref> <ref>PMID:11454748</ref> <ref>PMID:12062418</ref> <ref>PMID:12668726</ref> <ref>PMID:14523026</ref> <ref>PMID:15326198</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 18:		Line 19:
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1zb1 ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1zb1 ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	Proteins delivered to the lysosome or the yeast vacuole via late endosomes are sorted by the ESCRT complexes and by associated proteins, including Alix and its yeast homolog Bro1. Alix, Bro1, and several other late endosomal proteins share a conserved 160 residue Bro1 domain whose boundaries, structure, and function have not been characterized. The crystal structure of the Bro1 domain of Bro1 reveals a folded core of 367 residues. The extended Bro1 domain is necessary and sufficient for binding to the ESCRT-III subunit Snf7 and for the recruitment of Bro1 to late endosomes. The structure resembles a boomerang with its concave face filled in and contains a triple tetratricopeptide repeat domain as a substructure. Snf7 binds to a conserved hydrophobic patch on Bro1 that is required for protein complex formation and for the protein-sorting function of Bro1. These results define a conserved mechanism whereby Bro1 domain-containing proteins are targeted to endosomes by Snf7 and its orthologs.
-
-	Structural basis for endosomal targeting by the Bro1 domain.,Kim J, Sitaraman S, Hierro A, Beach BM, Odorizzi G, Hurley JH Dev Cell. 2005 Jun;8(6):937-47. PMID:15935782<ref>PMID:15935782</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 1zb1" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Atcc 18824]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Beach~~, B M~~]]	+	[[Category: Saccharomyces cerevisiae]]
-	[[Category: Hierro, A]]	+	[[Category: Beach BM]]
-	[[Category: Hurley~~, J H~~]]	+	[[Category: Hierro A]]
-	[[Category: Kim, J]]	+	[[Category: Hurley JH]]
-	[[Category: Odorizzi, G]]	+	[[Category: Kim J]]
-	[[Category: Sitaraman, S]]	+	[[Category: Odorizzi G]]
-	~~[[Category: Aip1]]~~	+	[[Category: Sitaraman S]]
-	~~[[Category: Bro1]]~~	+
-	~~[[Category: Bro1 domain]]~~	+
-	~~[[Category: Protein transport]]~~	+
-	~~[[Category: Snf7]]~~	+
-	~~[[Category: Trafficking~~]]	+

OCA at 08:18, 10 November 2021

OCA — Wed, 10 Nov 2021 08:18:59 GMT

←Older revision		Revision as of 08:18, 10 November 2021
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	<StructureSection load='1zb1' size='340' side='right'caption='[[1zb1]], [[Resolution\|resolution]] 1.95Å' scene=''>		<StructureSection load='1zb1' size='340' side='right'caption='[[1zb1]], [[Resolution\|resolution]] 1.95Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1zb1]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Atcc_18824 Atcc 18824]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZB1 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1ZB1 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1zb1]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Atcc_18824 Atcc 18824]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZB1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1ZB1 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1zb1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1zb1 OCA], [~~http~~://pdbe.org/1zb1 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1zb1 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1zb1 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1zb1 ProSAT]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1zb1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1zb1 OCA], [https://pdbe.org/1zb1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1zb1 RCSB], [https://www.ebi.ac.uk/pdbsum/1zb1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1zb1 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/BRO1_YEAST BRO1_YEAST]] Class E VPS protein involved in concentration and sorting of cargo proteins of the multivesicular body (MVB) for incorporation into intralumenal vesicles. Fusion between endosomes and the vacuole will then target the cargo proteins to the vacuolar lumen. Acts as an adapter that recruits the DOA4 deubiquitinase to the endosomes, leading to deubiquitination of cargo proteins prior to the lumenal sequestration. Its association to the endosomes depends on SNF7 and its dissociation requires VPS4. Interacts functionally with the Pkc1p-mitogen-activated protein kinase pathway.<ref>PMID:8649366</ref> <ref>PMID:11454748</ref> <ref>PMID:12062418</ref> <ref>PMID:12668726</ref> <ref>PMID:14523026</ref> <ref>PMID:15326198</ref>	+	[[https://www.uniprot.org/uniprot/BRO1_YEAST BRO1_YEAST]] Class E VPS protein involved in concentration and sorting of cargo proteins of the multivesicular body (MVB) for incorporation into intralumenal vesicles. Fusion between endosomes and the vacuole will then target the cargo proteins to the vacuolar lumen. Acts as an adapter that recruits the DOA4 deubiquitinase to the endosomes, leading to deubiquitination of cargo proteins prior to the lumenal sequestration. Its association to the endosomes depends on SNF7 and its dissociation requires VPS4. Interacts functionally with the Pkc1p-mitogen-activated protein kinase pathway.<ref>PMID:8649366</ref> <ref>PMID:11454748</ref> <ref>PMID:12062418</ref> <ref>PMID:12668726</ref> <ref>PMID:14523026</ref> <ref>PMID:15326198</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 09:38, 5 February 2020

OCA — Wed, 05 Feb 2020 09:38:22 GMT

←Older revision		Revision as of 09:38, 5 February 2020
Line 1:		Line 1:

	==Structure basis for endosomal targeting by the Bro1 domain==		==Structure basis for endosomal targeting by the Bro1 domain==
-	<StructureSection load='1zb1' size='340' side='right' caption='[[1zb1]], [[Resolution\|resolution]] 1.95Å' scene=''>	+	<StructureSection load='1zb1' size='340' side='right'caption='[[1zb1]], [[Resolution\|resolution]] 1.95Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1zb1]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_18824 Atcc 18824]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZB1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ZB1 FirstGlance]. <br>		<table><tr><td colspan='2'>[[1zb1]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_18824 Atcc 18824]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZB1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ZB1 FirstGlance]. <br>
Line 32:		Line 32:
	</StructureSection>		</StructureSection>
	[[Category: Atcc 18824]]		[[Category: Atcc 18824]]
		+	[[Category: Large Structures]]
	[[Category: Beach, B M]]		[[Category: Beach, B M]]
	[[Category: Hierro, A]]		[[Category: Hierro, A]]

OCA at 09:04, 2 May 2018

OCA — Wed, 02 May 2018 09:04:01 GMT

←Older revision		Revision as of 09:04, 2 May 2018
Line 1:		Line 1:
		+
	==Structure basis for endosomal targeting by the Bro1 domain==		==Structure basis for endosomal targeting by the Bro1 domain==
	<StructureSection load='1zb1' size='340' side='right' caption='[[1zb1]], [[Resolution\|resolution]] 1.95Å' scene=''>		<StructureSection load='1zb1' size='340' side='right' caption='[[1zb1]], [[Resolution\|resolution]] 1.95Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1zb1]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_18824 Atcc 18824]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZB1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ZB1 FirstGlance]. <br>		<table><tr><td colspan='2'>[[1zb1]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_18824 Atcc 18824]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZB1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ZB1 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1zb1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1zb1 OCA], [http://pdbe.org/1zb1 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1zb1 RCSB], [http://www.ebi.ac.uk/pdbsum/1zb1 PDBsum]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1zb1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1zb1 OCA], [http://pdbe.org/1zb1 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1zb1 RCSB], [http://www.ebi.ac.uk/pdbsum/1zb1 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1zb1 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
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	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/zb/1zb1_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/zb/1zb1_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>

OCA at 03:05, 10 February 2016

OCA — Wed, 10 Feb 2016 03:05:46 GMT

←Older revision		Revision as of 03:05, 10 February 2016
Line 15:		Line 15:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1zb1 ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 04:45, 11 September 2015

OCA — Fri, 11 Sep 2015 04:45:59 GMT

←Older revision		Revision as of 04:45, 11 September 2015
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	<StructureSection load='1zb1' size='340' side='right' caption='[[1zb1]], [[Resolution\|resolution]] 1.95Å' scene=''>		<StructureSection load='1zb1' size='340' side='right' caption='[[1zb1]], [[Resolution\|resolution]] 1.95Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1zb1]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Saccharomyces_cerevisiae Saccharomyces cerevisiae~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZB1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ZB1 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1zb1]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_18824 Atcc 18824]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZB1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ZB1 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1zb1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1zb1 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1zb1 RCSB], [http://www.ebi.ac.uk/pdbsum/1zb1 PDBsum]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1zb1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1zb1 OCA], [http://pdbe.org/1zb1 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1zb1 RCSB], [http://www.ebi.ac.uk/pdbsum/1zb1 PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 25:		Line 25:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1zb1" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Saccharomyces cerevisiae~~]]	+	[[Category: Atcc 18824]]
	[[Category: Beach, B M]]		[[Category: Beach, B M]]
	[[Category: Hierro, A]]		[[Category: Hierro, A]]

OCA at 11:21, 25 December 2014

OCA — Thu, 25 Dec 2014 11:21:56 GMT

←Older revision		Revision as of 11:21, 25 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1zb1]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZB1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ZB1 FirstGlance]. <br>		<table><tr><td colspan='2'>[[1zb1]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZB1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ZB1 FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1zb1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1zb1 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1zb1 RCSB], [http://www.ebi.ac.uk/pdbsum/1zb1 PDBsum]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1zb1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1zb1 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1zb1 RCSB], [http://www.ebi.ac.uk/pdbsum/1zb1 PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/BRO1_YEAST BRO1_YEAST]] Class E VPS protein involved in concentration and sorting of cargo proteins of the multivesicular body (MVB) for incorporation into intralumenal vesicles. Fusion between endosomes and the vacuole will then target the cargo proteins to the vacuolar lumen. Acts as an adapter that recruits the DOA4 deubiquitinase to the endosomes, leading to deubiquitination of cargo proteins prior to the lumenal sequestration. Its association to the endosomes depends on SNF7 and its dissociation requires VPS4. Interacts functionally with the Pkc1p-mitogen-activated protein kinase pathway.<ref>PMID:8649366</ref> <ref>PMID:11454748</ref> <ref>PMID:12062418</ref> <ref>PMID:12668726</ref> <ref>PMID:14523026</ref> <ref>PMID:15326198</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 28:		Line 30:
	</StructureSection>		</StructureSection>
	[[Category: Saccharomyces cerevisiae]]		[[Category: Saccharomyces cerevisiae]]
-	[[Category: Beach, B M.]]	+	[[Category: Beach, B M]]
-	[[Category: Hierro, A.]]	+	[[Category: Hierro, A]]
-	[[Category: Hurley, J H.]]	+	[[Category: Hurley, J H]]
-	[[Category: Kim, J.]]	+	[[Category: Kim, J]]
-	[[Category: Odorizzi, G.]]	+	[[Category: Odorizzi, G]]
-	[[Category: Sitaraman, S.]]	+	[[Category: Sitaraman, S]]
	[[Category: Aip1]]		[[Category: Aip1]]
	[[Category: Bro1]]		[[Category: Bro1]]

OCA at 18:33, 29 September 2014

OCA — Mon, 29 Sep 2014 18:33:55 GMT

←Older revision		Revision as of 18:33, 29 September 2014
Line 1:		Line 1:
-	[[~~Image~~:1zb1.~~png\|left~~\|200px]]	+	==Structure basis for endosomal targeting by the Bro1 domain==
		+	<StructureSection load='1zb1' size='340' side='right' caption='[[1zb1]], [[Resolution\|resolution]] 1.95Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1zb1]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZB1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ZB1 FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1zb1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1zb1 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1zb1 RCSB], [http://www.ebi.ac.uk/pdbsum/1zb1 PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/zb/1zb1_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Proteins delivered to the lysosome or the yeast vacuole via late endosomes are sorted by the ESCRT complexes and by associated proteins, including Alix and its yeast homolog Bro1. Alix, Bro1, and several other late endosomal proteins share a conserved 160 residue Bro1 domain whose boundaries, structure, and function have not been characterized. The crystal structure of the Bro1 domain of Bro1 reveals a folded core of 367 residues. The extended Bro1 domain is necessary and sufficient for binding to the ESCRT-III subunit Snf7 and for the recruitment of Bro1 to late endosomes. The structure resembles a boomerang with its concave face filled in and contains a triple tetratricopeptide repeat domain as a substructure. Snf7 binds to a conserved hydrophobic patch on Bro1 that is required for protein complex formation and for the protein-sorting function of Bro1. These results define a conserved mechanism whereby Bro1 domain-containing proteins are targeted to endosomes by Snf7 and its orthologs.

-	~~{{STRUCTURE_1zb1\| PDB=1zb1 \| SCENE= }}~~	+	Structural basis for endosomal targeting by the Bro1 domain.,Kim J, Sitaraman S, Hierro A, Beach BM, Odorizzi G, Hurley JH Dev Cell. 2005 Jun;8(6):937-47. PMID:15935782<ref>PMID:15935782</ref>

-	~~===Structure basis for endosomal targeting by~~ the ~~Bro1 domain===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_15935782}}~~	+	== References ==
-		+	<references/>
-	~~==About this Structure==~~	+	__TOC__
-	~~[[1zb1]] is a 2 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=1ZB1 OCA].~~	+	</StructureSection>
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:015935782<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Saccharomyces cerevisiae]]		[[Category: Saccharomyces cerevisiae]]
	[[Category: Beach, B M.]]		[[Category: Beach, B M.]]

OCA: Protected "1zb1" [edit=sysop:move=sysop]

OCA — Sat, 05 Jan 2013 17:22:08 GMT

Protected "1zb1" [edit=sysop:move=sysop]

←Older revision

Revision as of 17:22, 5 January 2013

OCA at 17:22, 5 January 2013

OCA — Sat, 05 Jan 2013 17:22:05 GMT

←Older revision		Revision as of 17:22, 5 January 2013
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-	{{Seed}}
	[[Image:1zb1.png\|left\|200px]]		[[Image:1zb1.png\|left\|200px]]

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	{{STRUCTURE_1zb1\| PDB=1zb1 \| SCENE= }}		{{STRUCTURE_1zb1\| PDB=1zb1 \| SCENE= }}

	===Structure basis for endosomal targeting by the Bro1 domain===		===Structure basis for endosomal targeting by the Bro1 domain===

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	{{ABSTRACT_PUBMED_15935782}}		{{ABSTRACT_PUBMED_15935782}}

	==About this Structure==		==About this Structure==
-	~~1ZB1~~ is a 2 ~~chains~~ structure ~~of sequences~~ from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZB1 OCA].	+	[[1zb1]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZB1 OCA].

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~15935782~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:015935782</ref><references group="xtra"/>
	[[Category: Saccharomyces cerevisiae]]		[[Category: Saccharomyces cerevisiae]]
	[[Category: Beach, B M.]]		[[Category: Beach, B M.]]
Line 34:		Line 22:
	[[Category: Bro1]]		[[Category: Bro1]]
	[[Category: Bro1 domain]]		[[Category: Bro1 domain]]
		+	[[Category: Protein transport]]
	[[Category: Snf7]]		[[Category: Snf7]]
	[[Category: Trafficking]]		[[Category: Trafficking]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 14:55:37 2009''