1zl6 - Revision history

OCA at 07:10, 23 August 2023

OCA — Wed, 23 Aug 2023 07:10:16 GMT

←Older revision		Revision as of 07:10, 23 August 2023
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	<StructureSection load='1zl6' size='340' side='right'caption='[[1zl6]], [[Resolution\|resolution]] 2.40Å' scene=''>		<StructureSection load='1zl6' size='340' side='right'caption='[[1zl6]], [[Resolution\|resolution]] 2.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1zl6]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~"bacillus_botulinus"_van_ermengem_1896 "bacillus botulinus" van ermengem 1896~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZL6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1ZL6 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1zl6]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Clostridium_botulinum Clostridium botulinum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZL6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1ZL6 FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>~~, ~~<scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.4Å</td></tr>
-	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"><~~div style~~='~~overflow~~: ~~auto; max-height: 3em;~~'>~~[[1t3a\|1t3a]], [[1t3c\|1t3c]], [[1zkw\|1zkw]], [[1zkx\|1zkx]], [[1zl5\|1zl5]]~~</~~div~~><~~/td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-	~~<tr id~~='~~activity'><td class~~=~~"sblockLbl"><b>Activity~~:~~</b></td><td class="sblockDat"><span class='plainlinks~~'>~~[https://en.wikipedia.org/wiki/Bontoxilysin Bontoxilysin], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.24.69 3.4.24.69]~~ </~~span~~></td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1zl6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1zl6 OCA], [https://pdbe.org/1zl6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1zl6 RCSB], [https://www.ebi.ac.uk/pdbsum/1zl6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1zl6 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1zl6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1zl6 OCA], [https://pdbe.org/1zl6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1zl6 RCSB], [https://www.ebi.ac.uk/pdbsum/1zl6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1zl6 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/BXE_CLOBO BXE_CLOBO]] Botulinum toxin acts by inhibiting neurotransmitter release. It binds to peripheral neuronal synapses, is internalized and moves by retrograde transport up the axon into the spinal cord where it can move between postsynaptic and presynaptic neurons. It inhibits neurotransmitter release by acting as a zinc endopeptidase that catalyzes the hydrolysis of the 180-Arg-\|-Ile-181 bond in SNAP-25.	+	[https://www.uniprot.org/uniprot/BXE_CLOBO BXE_CLOBO] Botulinum toxin acts by inhibiting neurotransmitter release. It binds to peripheral neuronal synapses, is internalized and moves by retrograde transport up the axon into the spinal cord where it can move between postsynaptic and presynaptic neurons. It inhibits neurotransmitter release by acting as a zinc endopeptidase that catalyzes the hydrolysis of the 180-Arg-\|-Ile-181 bond in SNAP-25.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Bacillus botulinus van ermengem 1896]]~~	+	[[Category: Clostridium botulinum]]
-	~~[[Category: Bontoxilysin~~]]	+
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Agarwal, R]]	+	[[Category: Agarwal R]]
-	[[Category: Binz, T]]	+	[[Category: Binz T]]
-	[[Category: Swaminathan, S]]	+	[[Category: Swaminathan S]]
-	~~[[Category: Botulinum neurotoxin e]]~~	+
-	~~[[Category: Catalytic domain]]~~	+
-	~~[[Category: Hydrolase]]~~	+
-	~~[[Category: Light chain]]~~	+
-	~~[[Category: Tyr350ala mutant~~]]	+

OCA at 06:09, 28 April 2021

OCA — Wed, 28 Apr 2021 06:09:16 GMT

←Older revision		Revision as of 06:09, 28 April 2021
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	<StructureSection load='1zl6' size='340' side='right'caption='[[1zl6]], [[Resolution\|resolution]] 2.40Å' scene=''>		<StructureSection load='1zl6' size='340' side='right'caption='[[1zl6]], [[Resolution\|resolution]] 2.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1zl6]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/"bacillus_botulinus"_van_ermengem_1896 "bacillus botulinus" van ermengem 1896]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZL6 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1ZL6 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1zl6]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/"bacillus_botulinus"_van_ermengem_1896 "bacillus botulinus" van ermengem 1896]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZL6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1ZL6 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1t3a\|1t3a]], [[1t3c\|1t3c]], [[1zkw\|1zkw]], [[1zkx\|1zkx]], [[1zl5\|1zl5]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1t3a\|1t3a]], [[1t3c\|1t3c]], [[1zkw\|1zkw]], [[1zkx\|1zkx]], [[1zl5\|1zl5]]</div></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Bontoxilysin Bontoxilysin], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.24.69 3.4.24.69] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Bontoxilysin Bontoxilysin], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.24.69 3.4.24.69] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1zl6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1zl6 OCA], [~~http~~://pdbe.org/1zl6 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1zl6 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1zl6 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1zl6 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1zl6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1zl6 OCA], [https://pdbe.org/1zl6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1zl6 RCSB], [https://www.ebi.ac.uk/pdbsum/1zl6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1zl6 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/BXE_CLOBO BXE_CLOBO]] Botulinum toxin acts by inhibiting neurotransmitter release. It binds to peripheral neuronal synapses, is internalized and moves by retrograde transport up the axon into the spinal cord where it can move between postsynaptic and presynaptic neurons. It inhibits neurotransmitter release by acting as a zinc endopeptidase that catalyzes the hydrolysis of the 180-Arg-\|-Ile-181 bond in SNAP-25.	+	[[https://www.uniprot.org/uniprot/BXE_CLOBO BXE_CLOBO]] Botulinum toxin acts by inhibiting neurotransmitter release. It binds to peripheral neuronal synapses, is internalized and moves by retrograde transport up the axon into the spinal cord where it can move between postsynaptic and presynaptic neurons. It inhibits neurotransmitter release by acting as a zinc endopeptidase that catalyzes the hydrolysis of the 180-Arg-\|-Ile-181 bond in SNAP-25.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 09:02, 24 July 2019

OCA — Wed, 24 Jul 2019 09:02:02 GMT

←Older revision		Revision as of 09:02, 24 July 2019
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	==Crystal structure of Tyr350Ala mutant of Clostridium botulinum neurotoxin E catalytic domain==		==Crystal structure of Tyr350Ala mutant of Clostridium botulinum neurotoxin E catalytic domain==
-	<StructureSection load='1zl6' size='340' side='right' caption='[[1zl6]], [[Resolution\|resolution]] 2.40Å' scene=''>	+	<StructureSection load='1zl6' size='340' side='right'caption='[[1zl6]], [[Resolution\|resolution]] 2.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1zl6]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_botulinus"_van_ermengem_1896 "bacillus botulinus" van ermengem 1896]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZL6 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ZL6 FirstGlance]. <br>		<table><tr><td colspan='2'>[[1zl6]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_botulinus"_van_ermengem_1896 "bacillus botulinus" van ermengem 1896]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZL6 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ZL6 FirstGlance]. <br>
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	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/zl/1zl6_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/zl/1zl6_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
Line 32:		Line 32:

	==See Also==		==See Also==
-	*[[Botulinum neurotoxin\|Botulinum neurotoxin]]	+	*[[Botulinum neurotoxin 3D structures\|Botulinum neurotoxin 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
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	[[Category: Bacillus botulinus van ermengem 1896]]		[[Category: Bacillus botulinus van ermengem 1896]]
	[[Category: Bontoxilysin]]		[[Category: Bontoxilysin]]
		+	[[Category: Large Structures]]
	[[Category: Agarwal, R]]		[[Category: Agarwal, R]]
	[[Category: Binz, T]]		[[Category: Binz, T]]

OCA at 12:37, 12 October 2017

OCA — Thu, 12 Oct 2017 12:37:50 GMT

←Older revision		Revision as of 12:37, 12 October 2017
Line 1:		Line 1:
		+
	==Crystal structure of Tyr350Ala mutant of Clostridium botulinum neurotoxin E catalytic domain==		==Crystal structure of Tyr350Ala mutant of Clostridium botulinum neurotoxin E catalytic domain==
	<StructureSection load='1zl6' size='340' side='right' caption='[[1zl6]], [[Resolution\|resolution]] 2.40Å' scene=''>		<StructureSection load='1zl6' size='340' side='right' caption='[[1zl6]], [[Resolution\|resolution]] 2.40Å' scene=''>
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	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1t3a\|1t3a]], [[1t3c\|1t3c]], [[1zkw\|1zkw]], [[1zkx\|1zkx]], [[1zl5\|1zl5]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1t3a\|1t3a]], [[1t3c\|1t3c]], [[1zkw\|1zkw]], [[1zkx\|1zkx]], [[1zl5\|1zl5]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Bontoxilysin Bontoxilysin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.24.69 3.4.24.69] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Bontoxilysin Bontoxilysin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.24.69 3.4.24.69] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1zl6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1zl6 OCA], [http://pdbe.org/1zl6 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1zl6 RCSB], [http://www.ebi.ac.uk/pdbsum/1zl6 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1zl6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1zl6 OCA], [http://pdbe.org/1zl6 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1zl6 RCSB], [http://www.ebi.ac.uk/pdbsum/1zl6 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1zl6 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==

OCA at 06:19, 7 February 2016

OCA — Sun, 07 Feb 2016 06:19:42 GMT

←Older revision		Revision as of 06:19, 7 February 2016
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	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1zl6 ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 22:40, 9 September 2015

OCA — Wed, 09 Sep 2015 22:40:01 GMT

←Older revision		Revision as of 22:40, 9 September 2015
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	<StructureSection load='1zl6' size='340' side='right' caption='[[1zl6]], [[Resolution\|resolution]] 2.40Å' scene=''>		<StructureSection load='1zl6' size='340' side='right' caption='[[1zl6]], [[Resolution\|resolution]] 2.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1zl6]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Clostridium_botulinum Clostridium botulinum~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZL6 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ZL6 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1zl6]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_botulinus"_van_ermengem_1896 "bacillus botulinus" van ermengem 1896]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZL6 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ZL6 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1t3a\|1t3a]], [[1t3c\|1t3c]], [[1zkw\|1zkw]], [[1zkx\|1zkx]], [[1zl5\|1zl5]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1t3a\|1t3a]], [[1t3c\|1t3c]], [[1zkw\|1zkw]], [[1zkx\|1zkx]], [[1zl5\|1zl5]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Bontoxilysin Bontoxilysin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.24.69 3.4.24.69] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Bontoxilysin Bontoxilysin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.24.69 3.4.24.69] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1zl6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1zl6 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1zl6 RCSB], [http://www.ebi.ac.uk/pdbsum/1zl6 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1zl6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1zl6 OCA], [http://pdbe.org/1zl6 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1zl6 RCSB], [http://www.ebi.ac.uk/pdbsum/1zl6 PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
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	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1zl6" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Botulinum neurotoxin\|Botulinum neurotoxin]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Bacillus botulinus van ermengem 1896]]
	[[Category: Bontoxilysin]]		[[Category: Bontoxilysin]]
-	[[Category: Clostridium botulinum]]
	[[Category: Agarwal, R]]		[[Category: Agarwal, R]]
	[[Category: Binz, T]]		[[Category: Binz, T]]

OCA at 08:34, 24 December 2014

OCA — Wed, 24 Dec 2014 08:34:42 GMT

←Older revision		Revision as of 08:34, 24 December 2014
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	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1zl6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1zl6 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1zl6 RCSB], [http://www.ebi.ac.uk/pdbsum/1zl6 PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1zl6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1zl6 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1zl6 RCSB], [http://www.ebi.ac.uk/pdbsum/1zl6 PDBsum]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/BXE_CLOBO BXE_CLOBO]] Botulinum toxin acts by inhibiting neurotransmitter release. It binds to peripheral neuronal synapses, is internalized and moves by retrograde transport up the axon into the spinal cord where it can move between postsynaptic and presynaptic neurons. It inhibits neurotransmitter release by acting as a zinc endopeptidase that catalyzes the hydrolysis of the 180-Arg-\|-Ile-181 bond in SNAP-25.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 08:32, 8 December 2014

OCA — Mon, 08 Dec 2014 08:32:36 GMT

←Older revision		Revision as of 08:32, 8 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1zl6]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Clostridium_botulinum Clostridium botulinum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZL6 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ZL6 FirstGlance]. <br>		<table><tr><td colspan='2'>[[1zl6]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Clostridium_botulinum Clostridium botulinum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZL6 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ZL6 FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1t3a\|1t3a]], [[1t3c\|1t3c]], [[1zkw\|1zkw]], [[1zkx\|1zkx]], [[1zl5\|1zl5]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1t3a\|1t3a]], [[1t3c\|1t3c]], [[1zkw\|1zkw]], [[1zkx\|1zkx]], [[1zl5\|1zl5]]</td></tr>
-	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Bontoxilysin Bontoxilysin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.24.69 3.4.24.69] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Bontoxilysin Bontoxilysin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.24.69 3.4.24.69] </span></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1zl6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1zl6 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1zl6 RCSB], [http://www.ebi.ac.uk/pdbsum/1zl6 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1zl6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1zl6 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1zl6 RCSB], [http://www.ebi.ac.uk/pdbsum/1zl6 PDBsum]</span></td></tr>
-	<table>	+	</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 32:		Line 32:
	[[Category: Bontoxilysin]]		[[Category: Bontoxilysin]]
	[[Category: Clostridium botulinum]]		[[Category: Clostridium botulinum]]
-	[[Category: Agarwal, R.]]	+	[[Category: Agarwal, R]]
-	[[Category: Binz, T.]]	+	[[Category: Binz, T]]
-	[[Category: Swaminathan, S.]]	+	[[Category: Swaminathan, S]]
	[[Category: Botulinum neurotoxin e]]		[[Category: Botulinum neurotoxin e]]
	[[Category: Catalytic domain]]		[[Category: Catalytic domain]]

OCA at 19:25, 29 September 2014

OCA — Mon, 29 Sep 2014 19:25:08 GMT

←Older revision		Revision as of 19:25, 29 September 2014
Line 1:		Line 1:
-	[[~~Image:~~1zl6.~~png~~\|~~left~~\|200px]]	+	==Crystal structure of Tyr350Ala mutant of Clostridium botulinum neurotoxin E catalytic domain==
		+	<StructureSection load='1zl6' size='340' side='right' caption='[[1zl6]], [[Resolution\|resolution]] 2.40Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1zl6]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Clostridium_botulinum Clostridium botulinum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZL6 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ZL6 FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1t3a\|1t3a]], [[1t3c\|1t3c]], [[1zkw\|1zkw]], [[1zkx\|1zkx]], [[1zl5\|1zl5]]</td></tr>
		+	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Bontoxilysin Bontoxilysin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.24.69 3.4.24.69] </span></td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1zl6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1zl6 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1zl6 RCSB], [http://www.ebi.ac.uk/pdbsum/1zl6 PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/zl/1zl6_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Clostridial neurotoxins comprising the seven serotypes of botulinum neurotoxins and tetanus neurotoxin are the most potent toxins known to humans. Their potency coupled with their specificity and selectivity underscores the importance in understanding their mechanism of action in order to develop a strategy for designing counter measures against them. To develop an effective vaccine against the toxin, it is imperative to achieve an inactive form of the protein which preserves the overall conformation and immunogenicity. Inactive mutants can be achieved either by targeting active site residues or by modifying the surface charges farther away from the active site. The latter affects the long-range forces such as electrostatic potentials in a subtle way without disturbing the structural integrity of the toxin causing some drastic changes in the activity/environment. Here we report structural and biochemical analysis on several mutations on Clostridium botulinum neurotoxin type E light chain with at least two producing dramatic effects: Glu335Gln causes the toxin to transform into a persistent apoenzyme devoid of zinc, and Tyr350Ala has no hydrolytic activity. The structural analysis of several mutants has led to a better understanding of the catalytic mechanism of this family of proteins. The residues forming the S1' subsite have been identified by comparing this structure with a thermolysin-inhibitor complex structure.

-	~~{{STRUCTURE_1zl6\| PDB=1zl6 \| SCENE= }}~~	+	Analysis of active site residues of botulinum neurotoxin E by mutational, functional, and structural studies: Glu335Gln is an apoenzyme.,Agarwal R, Binz T, Swaminathan S Biochemistry. 2005 Jun 14;44(23):8291-302. PMID:15938619<ref>PMID:15938619</ref>

-	~~===Crystal structure~~ of ~~Tyr350Ala mutant of Clostridium botulinum neurotoxin E catalytic domain===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_15938619}}~~	+	== References ==
-		+	<references/>
-	~~==About this Structure==~~	+	__TOC__
-	~~[[1zl6]] is a 2 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Clostridium_botulinum Clostridium botulinum]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=1ZL6 OCA].~~	+	</StructureSection>
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:015938619<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Bontoxilysin]]		[[Category: Bontoxilysin]]
	[[Category: Clostridium botulinum]]		[[Category: Clostridium botulinum]]

OCA at 17:06, 5 January 2013

OCA — Sat, 05 Jan 2013 17:06:12 GMT

←Older revision		Revision as of 17:06, 5 January 2013
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	[[Image:1zl6.png\|left\|200px]]		[[Image:1zl6.png\|left\|200px]]

-	<!--
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	{{STRUCTURE_1zl6\| PDB=1zl6 \| SCENE= }}		{{STRUCTURE_1zl6\| PDB=1zl6 \| SCENE= }}

	===Crystal structure of Tyr350Ala mutant of Clostridium botulinum neurotoxin E catalytic domain===		===Crystal structure of Tyr350Ala mutant of Clostridium botulinum neurotoxin E catalytic domain===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_15938619}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 15938619 is the PubMed ID number.
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	{{ABSTRACT_PUBMED_15938619}}		{{ABSTRACT_PUBMED_15938619}}

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	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~15938619~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:015938619</ref><references group="xtra"/>
	[[Category: Bontoxilysin]]		[[Category: Bontoxilysin]]
	[[Category: Clostridium botulinum]]		[[Category: Clostridium botulinum]]