1zlw - Revision history

OCA: Redirecting to 6mnf

OCA — Wed, 31 Oct 2018 06:42:44 GMT

Redirecting to 6mnf

←Older revision		Revision as of 06:42, 31 October 2018
Line 1:		Line 1:
-		+	#REDIRECT [[6mnf]] This PDB entry is obsolete and replaced by 6mnf
-	~~==Fab 2G12 + Man8==~~	+
-	~~<StructureSection load='1zlw' size='340' side='right' caption='~~[[~~1zlw]], [[Resolution\|resolution]] 2.85Å' scene=''>~~	+
-	~~== Structural highlights ==~~	+
-	~~<table><tr><td colspan='2'>[[1zlw~~]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZLW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ZLW FirstGlance]. <br>	+
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene></td></tr>	+
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1om3\|1om3]], [[1op3\|1op3]], [[1op5\|1op5]], [[1zls\|1zls]], [[1zlu\|1zlu]], [[1zlv\|1zlv]]</td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1zlw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1zlw OCA], [http://pdbe.org/1zlw PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1zlw RCSB], [http://www.ebi.ac.uk/pdbsum/1zlw PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1zlw ProSAT]</span></td></tr>	+
-	~~</table>~~	+
-	~~== Evolutionary Conservation ==~~	+
-	~~[[Image:Consurf_key_small.gif\|200px\|right]]~~	+
-	~~Check<jmol>~~	+
-	~~<jmolCheckbox>~~	+
-	~~<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/zl/1zlw_consurf.spt"</scriptWhenChecked>~~	+
-	~~<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>~~	+
-	~~<text>to colour the structure~~ by ~~Evolutionary Conservation</text>~~	+
-	~~</jmolCheckbox>~~	+
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1zlw ConSurf].	+
-	~~<div style="clear:both"></div>~~	+
-	~~<div style="background-color:#fffaf0;">~~	+
-	~~== Publication Abstract from PubMed ==~~	+
-	Human antibody 2G12 neutralizes a broad range of HIV-1 isolates. Hence, molecular characterization of its epitope, which corresponds to a conserved cluster of oligomannoses on the viral envelope glycoprotein gp120, is a high priority in HIV vaccine design. A prior crystal structure of 2G12 in complex with Man(9)GlcNAc(2) highlighted the central importance of the D1 arm in antibody binding. To characterize the specificity of 2G12 more precisely, we performed solution-phase ELISA, carbohydrate microarray analysis, and cocrystallized Fab 2G12 with four different oligomannose derivatives (Man(4), Man(5), Man(7), and Man(8)) that compete with gp120 for binding to 2G12. Our combined studies reveal that 2G12 is capable of binding both the D1 and D3 arms of the Man(9)GlcNAc(2) moiety, which would provide more flexibility to make the required multivalent interactions between the antibody and the gp120 oligomannose cluster than thought previously. These results have important consequences for the design of immunogens to elicit 2G12-like neutralizing antibodies as a component of an HIV vaccine.	+
-		+
-	Dissection of the carbohydrate specificity of the broadly neutralizing anti-HIV-1 antibody 2G12.,Calarese DA, Lee HK, Huang CY, Best MD, Astronomo RD, Stanfield RL, Katinger H, Burton DR, Wong CH, Wilson IA Proc Natl Acad Sci U S A. 2005 Sep 20;102(38):13372-7. Epub 2005 Sep 7. PMID:16174734<ref>PMID:16174734</ref>	+
-		+
-	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~	+
-	~~</div>~~	+
-	~~<div class="pdbe-citations 1zlw" style="background-color:#fffaf0;"></div>~~	+
-	~~== References ==~~	+
-	~~<references/>~~	+
-	~~__TOC__~~	+
-	~~</StructureSection>~~	+
-	~~[[Category: Human]]~~	+
-	~~[[Category: Astronomo, R D]]~~	+
-	~~[[Category: Best, M D]]~~	+
-	~~[[Category: Burton, D R]]~~	+
-	~~[[Category: Calarese, D A]]~~	+
-	~~[[Category: Lee, H K]]~~	+
-	~~[[Category: Stanfield, R L]]~~	+
-	~~[[Category: Wilson, I A]]~~	+
-	~~[[Category: Wong, C H]]~~	+
-	~~[[Category: Anti-carbohydrate]]~~	+
-	~~[[Category: Domain-swapping]]~~	+
-	~~[[Category: Hiv neutralizing antibody]]~~	+
-	~~[[Category: Immune system]]~~	+

OCA at 09:02, 2 May 2018

OCA — Wed, 02 May 2018 09:02:01 GMT

←Older revision		Revision as of 09:02, 2 May 2018
Line 1:		Line 1:
		+
	==Fab 2G12 + Man8==		==Fab 2G12 + Man8==
	<StructureSection load='1zlw' size='340' side='right' caption='[[1zlw]], [[Resolution\|resolution]] 2.85Å' scene=''>		<StructureSection load='1zlw' size='340' side='right' caption='[[1zlw]], [[Resolution\|resolution]] 2.85Å' scene=''>
Line 5:		Line 6:
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1om3\|1om3]], [[1op3\|1op3]], [[1op5\|1op5]], [[1zls\|1zls]], [[1zlu\|1zlu]], [[1zlv\|1zlv]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1om3\|1om3]], [[1op3\|1op3]], [[1op5\|1op5]], [[1zls\|1zls]], [[1zlu\|1zlu]], [[1zlv\|1zlv]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1zlw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1zlw OCA], [http://pdbe.org/1zlw PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1zlw RCSB], [http://www.ebi.ac.uk/pdbsum/1zlw PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1zlw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1zlw OCA], [http://pdbe.org/1zlw PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1zlw RCSB], [http://www.ebi.ac.uk/pdbsum/1zlw PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1zlw ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 11:		Line 12:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/zl/1zlw_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/zl/1zlw_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
Line 26:		Line 27:
	</div>		</div>
	<div class="pdbe-citations 1zlw" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 1zlw" style="background-color:#fffaf0;"></div>
-
-	==See Also==
-	*[[Antibody\|Antibody]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 16:50, 7 February 2016

OCA — Sun, 07 Feb 2016 16:50:26 GMT

←Older revision		Revision as of 16:50, 7 February 2016
Line 15:		Line 15:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1zlw ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 03:23, 10 September 2015

OCA — Thu, 10 Sep 2015 03:23:57 GMT

←Older revision		Revision as of 03:23, 10 September 2015
Line 2:		Line 2:
	<StructureSection load='1zlw' size='340' side='right' caption='[[1zlw]], [[Resolution\|resolution]] 2.85Å' scene=''>		<StructureSection load='1zlw' size='340' side='right' caption='[[1zlw]], [[Resolution\|resolution]] 2.85Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1zlw]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Homo_sapiens Homo sapiens~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZLW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ZLW FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1zlw]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZLW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ZLW FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1om3\|1om3]], [[1op3\|1op3]], [[1op5\|1op5]], [[1zls\|1zls]], [[1zlu\|1zlu]], [[1zlv\|1zlv]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1om3\|1om3]], [[1op3\|1op3]], [[1op5\|1op5]], [[1zls\|1zls]], [[1zlu\|1zlu]], [[1zlv\|1zlv]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1zlw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1zlw OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1zlw RCSB], [http://www.ebi.ac.uk/pdbsum/1zlw PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1zlw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1zlw OCA], [http://pdbe.org/1zlw PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1zlw RCSB], [http://www.ebi.ac.uk/pdbsum/1zlw PDBsum]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 25:		Line 25:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1zlw" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 32:		Line 33:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Homo sapiens~~]]	+	[[Category: Human]]
	[[Category: Astronomo, R D]]		[[Category: Astronomo, R D]]
	[[Category: Best, M D]]		[[Category: Best, M D]]

OCA at 10:15, 8 January 2015

OCA — Thu, 08 Jan 2015 10:15:27 GMT

←Older revision		Revision as of 10:15, 8 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1zlw]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZLW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ZLW FirstGlance]. <br>		<table><tr><td colspan='2'>[[1zlw]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZLW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ZLW FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1om3\|1om3]], [[1op3\|1op3]], [[1op5\|1op5]], [[1zls\|1zls]], [[1zlu\|1zlu]], [[1zlv\|1zlv]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1om3\|1om3]], [[1op3\|1op3]], [[1op5\|1op5]], [[1zls\|1zls]], [[1zlu\|1zlu]], [[1zlv\|1zlv]]</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1zlw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1zlw OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1zlw RCSB], [http://www.ebi.ac.uk/pdbsum/1zlw PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1zlw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1zlw OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1zlw RCSB], [http://www.ebi.ac.uk/pdbsum/1zlw PDBsum]</span></td></tr>
-	<table>	+	</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 33:		Line 33:
	</StructureSection>		</StructureSection>
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
-	[[Category: Astronomo, R D.]]	+	[[Category: Astronomo, R D]]
-	[[Category: Best, M D.]]	+	[[Category: Best, M D]]
-	[[Category: Burton, D R.]]	+	[[Category: Burton, D R]]
-	[[Category: Calarese, D A.]]	+	[[Category: Calarese, D A]]
-	[[Category: Lee, H K.]]	+	[[Category: Lee, H K]]
-	[[Category: Stanfield, R L.]]	+	[[Category: Stanfield, R L]]
-	[[Category: Wilson, I A.]]	+	[[Category: Wilson, I A]]
-	[[Category: Wong, C H.]]	+	[[Category: Wong, C H]]
	[[Category: Anti-carbohydrate]]		[[Category: Anti-carbohydrate]]
	[[Category: Domain-swapping]]		[[Category: Domain-swapping]]
	[[Category: Hiv neutralizing antibody]]		[[Category: Hiv neutralizing antibody]]
	[[Category: Immune system]]		[[Category: Immune system]]

OCA at 09:53, 29 September 2014

OCA — Mon, 29 Sep 2014 09:53:18 GMT

←Older revision		Revision as of 09:53, 29 September 2014
Line 1:		Line 1:
-	[[~~Image:~~1zlw.~~png~~\|~~left~~\|200px]]	+	==Fab 2G12 + Man8==
		+	<StructureSection load='1zlw' size='340' side='right' caption='[[1zlw]], [[Resolution\|resolution]] 2.85Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1zlw]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZLW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ZLW FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1om3\|1om3]], [[1op3\|1op3]], [[1op5\|1op5]], [[1zls\|1zls]], [[1zlu\|1zlu]], [[1zlv\|1zlv]]</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1zlw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1zlw OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1zlw RCSB], [http://www.ebi.ac.uk/pdbsum/1zlw PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/zl/1zlw_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Human antibody 2G12 neutralizes a broad range of HIV-1 isolates. Hence, molecular characterization of its epitope, which corresponds to a conserved cluster of oligomannoses on the viral envelope glycoprotein gp120, is a high priority in HIV vaccine design. A prior crystal structure of 2G12 in complex with Man(9)GlcNAc(2) highlighted the central importance of the D1 arm in antibody binding. To characterize the specificity of 2G12 more precisely, we performed solution-phase ELISA, carbohydrate microarray analysis, and cocrystallized Fab 2G12 with four different oligomannose derivatives (Man(4), Man(5), Man(7), and Man(8)) that compete with gp120 for binding to 2G12. Our combined studies reveal that 2G12 is capable of binding both the D1 and D3 arms of the Man(9)GlcNAc(2) moiety, which would provide more flexibility to make the required multivalent interactions between the antibody and the gp120 oligomannose cluster than thought previously. These results have important consequences for the design of immunogens to elicit 2G12-like neutralizing antibodies as a component of an HIV vaccine.

-	~~{{STRUCTURE_1zlw\| PDB=1zlw \| SCENE= }}~~	+	Dissection of the carbohydrate specificity of the broadly neutralizing anti-HIV-1 antibody 2G12.,Calarese DA, Lee HK, Huang CY, Best MD, Astronomo RD, Stanfield RL, Katinger H, Burton DR, Wong CH, Wilson IA Proc Natl Acad Sci U S A. 2005 Sep 20;102(38):13372-7. Epub 2005 Sep 7. PMID:16174734<ref>PMID:16174734</ref>

-	~~===Fab 2G12 + Man8===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_16174734}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[1zlw]] is~~ a ~~4 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Homo_sapiens Homo sapiens]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=1ZLW OCA].~~	+

	==See Also==		==See Also==
	*[[Antibody\|Antibody]]		*[[Antibody\|Antibody]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	<~~ref group="xtra">PMID:016174734<~~/~~ref~~><~~references group="xtra"~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
	[[Category: Astronomo, R D.]]		[[Category: Astronomo, R D.]]

OCA at 07:16, 20 October 2012

OCA — Sat, 20 Oct 2012 07:16:59 GMT

←Older revision		Revision as of 07:16, 20 October 2012
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	==About this Structure==		==About this Structure==
-	[[1zlw]] is a 4 chain structure ~~of [[Antibody]]~~ with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZLW OCA].	+	[[1zlw]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZLW OCA].

	==See Also==		==See Also==

OCA: Protected "1zlw" [edit=sysop:move=sysop]

OCA — Wed, 25 Jul 2012 15:49:50 GMT

Protected "1zlw" [edit=sysop:move=sysop]

←Older revision

Revision as of 15:49, 25 July 2012

OCA at 15:49, 25 July 2012

OCA — Wed, 25 Jul 2012 15:49:48 GMT

←Older revision		Revision as of 15:49, 25 July 2012
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-	{{Seed}}
	[[Image:1zlw.png\|left\|200px]]		[[Image:1zlw.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_1zlw", creates the "Structure Box" on the page.
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	{{STRUCTURE_1zlw\| PDB=1zlw \| SCENE= }}		{{STRUCTURE_1zlw\| PDB=1zlw \| SCENE= }}

	===Fab 2G12 + Man8===		===Fab 2G12 + Man8===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_16174734}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 16174734 is the PubMed ID number.
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	{{ABSTRACT_PUBMED_16174734}}		{{ABSTRACT_PUBMED_16174734}}

	==About this Structure==		==About this Structure==
-	~~1ZLW~~ is a 4 ~~chains~~ structure of ~~sequences~~ from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZLW OCA].	+	[[1zlw]] is a 4 chain structure of [[Antibody]] with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZLW OCA].
		+
		+	==See Also==
		+	*[[Antibody\|Antibody]]

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~16174734~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:016174734</ref><references group="xtra"/>
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
	[[Category: Astronomo, R D.]]		[[Category: Astronomo, R D.]]
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	[[Category: Domain-swapping]]		[[Category: Domain-swapping]]
	[[Category: Hiv neutralizing antibody]]		[[Category: Hiv neutralizing antibody]]
-		+	[[Category: Immune system]]
-	~~''Page seeded by~~ [~~http~~:~~//oca.weizmann.ac.il/oca OCA~~ ] ~~on Mon Feb 16 15:14:17 2009''~~	+

OCA at 13:14, 16 February 2009

OCA — Mon, 16 Feb 2009 13:14:18 GMT

←Older revision		Revision as of 13:14, 16 February 2009
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	==About this Structure==		==About this Structure==
-	Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZLW OCA].	+	1ZLW is a 4 chains structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZLW OCA].

	==Reference==		==Reference==
-	Dissection of the carbohydrate specificity of the broadly neutralizing anti-HIV-1 antibody 2G12., Calarese DA, Lee HK, Huang CY, Best MD, Astronomo RD, Stanfield RL, Katinger H, Burton DR, Wong CH, Wilson IA, Proc Natl Acad Sci U S A. 2005 Sep 20;102(38):13372-7. Epub 2005 Sep 7. PMID:[~~http~~:~~//www.ncbi.nlm.nih.gov/pubmed/16174734 16174734~~]	+	<ref group="xtra">PMID:16174734</ref><references group="xtra"/>
		+	[[Category: Homo sapiens]]
	[[Category: Astronomo, R D.]]		[[Category: Astronomo, R D.]]
	[[Category: Best, M D.]]		[[Category: Best, M D.]]
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	[[Category: Hiv neutralizing antibody]]		[[Category: Hiv neutralizing antibody]]

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Sun Jul 27 18~~:04:~~41 2008~~''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Feb 16 15:14:17 2009''