1zn1 - Revision history

OCA at 09:05, 14 February 2024

OCA — Wed, 14 Feb 2024 09:05:54 GMT

←Older revision		Revision as of 09:05, 14 February 2024
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1zn1]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZN1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1ZN1 FirstGlance]. <br>		<table><tr><td colspan='2'>[[1zn1]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZN1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1ZN1 FirstGlance]. <br>
-	</td></tr><tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"~~><div style~~=~~'overflow: auto; max-height: 3em;'~~>~~[[1p6g\|1p6g]]~~, [[~~1p85~~\|~~1p85~~]]~~, [[1ek8\|1ek8]], [[1zn0\|1zn0]]</div>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution\|Resolution]] 14.1Å</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1zn1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1zn1 OCA], [https://pdbe.org/1zn1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1zn1 RCSB], [https://www.ebi.ac.uk/pdbsum/1zn1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1zn1 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1zn1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1zn1 OCA], [https://pdbe.org/1zn1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1zn1 RCSB], [https://www.ebi.ac.uk/pdbsum/1zn1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1zn1 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/RS12_ECOLI RS12_ECOLI]] With S4 and S5 plays an important role in translational accuracy.[HAMAP-Rule:MF_00403_B] Interacts with and stabilizes bases of the 16S rRNA that are involved in tRNA selection in the A site and with the mRNA backbone. Located at the interface of the 30S and 50S subunits, it traverses the body of the 30S subunit contacting proteins on the other side and probably holding the rRNA structure together. The combined cluster of proteins S8, S12 and S17 appears to hold together the shoulder and platform of the 30S subunit (By similarity).[HAMAP-Rule:MF_00403_B] Cryo-EM studies suggest that S12 contacts the EF-Tu bound tRNA in the A-site during codon-recognition. This contact is most likely broken as the aminoacyl-tRNA moves into the peptidyl transferase center in the 50S subunit.[HAMAP-Rule:MF_00403_B] [[https://www.uniprot.org/uniprot/RRF_ECOLI RRF_ECOLI]] Responsible for the release of ribosomes from messenger RNA at the termination of protein biosynthesis. May increase the efficiency of translation by recycling ribosomes from one round of translation to another.[HAMAP-Rule:MF_00040]	+	[https://www.uniprot.org/uniprot/RRF_ECOLI RRF_ECOLI] Responsible for the release of ribosomes from messenger RNA at the termination of protein biosynthesis. May increase the efficiency of translation by recycling ribosomes from one round of translation to another.[HAMAP-Rule:MF_00040]
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1zn1 ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1zn1 ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	Ribosome recycling, the disassembly of the posttermination complex after each round of protein synthesis, is an essential step in mRNA translation, but its mechanism has remained obscure. In eubacteria, recycling is catalyzed by RRF (ribosome recycling factor) and EF-G (elongation factor G). By using cryo-electron microscopy, we have obtained two density maps, one of the RRF bound posttermination complex and one of the 50S subunit bound with both EF-G and RRF. Comparing the two maps, we found domain I of RRF to be in the same orientation, while domain II in the EF-G-containing 50S subunit is extensively rotated (approximately 60 degrees) compared to its orientation in the 70S complex. Mapping the 50S conformation of RRF onto the 70S posttermination complex suggests that it can disrupt the intersubunit bridges B2a and B3, and thus effect a separation of the two subunits. These observations provide the structural basis for the mechanism by which the posttermination complex is split into subunits by the joint action of RRF and EF-G.
-
-	Mechanism for the disassembly of the posttermination complex inferred from cryo-EM studies.,Gao N, Zavialov AV, Li W, Sengupta J, Valle M, Gursky RP, Ehrenberg M, Frank J Mol Cell. 2005 Jun 10;18(6):663-74. PMID:15949441<ref>PMID:15949441</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 1zn1" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
	*[[Ribosomal protein S12\|Ribosomal protein S12]]		*[[Ribosomal protein S12\|Ribosomal protein S12]]
	*[[Ribosome recycling factor\|Ribosome recycling factor]]		*[[Ribosome recycling factor\|Ribosome recycling factor]]
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</SX>		</SX>
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Ehrenberg, M]]	+	[[Category: Ehrenberg M]]
-	[[Category: Frank, J]]	+	[[Category: Frank J]]
-	[[Category: Gao, N]]	+	[[Category: Gao N]]
-	[[Category: Gursky~~, R P~~]]	+	[[Category: Gursky RP]]
-	[[Category: Li, W]]	+	[[Category: Li W]]
-	[[Category: Sengupta, J]]	+	[[Category: Sengupta J]]
-	[[Category: Valle, M]]	+	[[Category: Valle M]]
-	[[Category: Zavialov~~, A V]]~~	+	[[Category: Zavialov AV]]
-	~~[[Category: Biosynthetic-structural protein-rna complex]]~~	+
-	~~[[Category: Elongation factor g]]~~	+
-	~~[[Category: Post-termination complex]]~~	+
-	~~[[Category: Ribosome recycling factor~~]]	+

OCA at 10:20, 12 January 2022

OCA — Wed, 12 Jan 2022 10:20:10 GMT

←Older revision		Revision as of 10:20, 12 January 2022
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	<SX load='1zn1' size='340' side='right' viewer='molstar' caption='[[1zn1]], [[Resolution\|resolution]] 14.10Å' scene=''>		<SX load='1zn1' size='340' side='right' viewer='molstar' caption='[[1zn1]], [[Resolution\|resolution]] 14.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1zn1]] is a 4 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZN1 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=1ZN1 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1zn1]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZN1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1ZN1 FirstGlance]. <br>
-	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1p6g\|1p6g]], [[1p85\|1p85]], [[1ek8\|1ek8]], [[1zn0\|1zn0]]</td></tr>	+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1p6g\|1p6g]], [[1p85\|1p85]], [[1ek8\|1ek8]], [[1zn0\|1zn0]]</div></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=1zn1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1zn1 OCA], [~~http~~://pdbe.org/1zn1 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1zn1 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1zn1 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1zn1 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1zn1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1zn1 OCA], [https://pdbe.org/1zn1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1zn1 RCSB], [https://www.ebi.ac.uk/pdbsum/1zn1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1zn1 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/RS12_ECOLI RS12_ECOLI]] With S4 and S5 plays an important role in translational accuracy.[HAMAP-Rule:MF_00403_B] Interacts with and stabilizes bases of the 16S rRNA that are involved in tRNA selection in the A site and with the mRNA backbone. Located at the interface of the 30S and 50S subunits, it traverses the body of the 30S subunit contacting proteins on the other side and probably holding the rRNA structure together. The combined cluster of proteins S8, S12 and S17 appears to hold together the shoulder and platform of the 30S subunit (By similarity).[HAMAP-Rule:MF_00403_B] Cryo-EM studies suggest that S12 contacts the EF-Tu bound tRNA in the A-site during codon-recognition. This contact is most likely broken as the aminoacyl-tRNA moves into the peptidyl transferase center in the 50S subunit.[HAMAP-Rule:MF_00403_B] [[~~http~~://www.uniprot.org/uniprot/RRF_ECOLI RRF_ECOLI]] Responsible for the release of ribosomes from messenger RNA at the termination of protein biosynthesis. May increase the efficiency of translation by recycling ribosomes from one round of translation to another.[HAMAP-Rule:MF_00040]	+	[[https://www.uniprot.org/uniprot/RS12_ECOLI RS12_ECOLI]] With S4 and S5 plays an important role in translational accuracy.[HAMAP-Rule:MF_00403_B] Interacts with and stabilizes bases of the 16S rRNA that are involved in tRNA selection in the A site and with the mRNA backbone. Located at the interface of the 30S and 50S subunits, it traverses the body of the 30S subunit contacting proteins on the other side and probably holding the rRNA structure together. The combined cluster of proteins S8, S12 and S17 appears to hold together the shoulder and platform of the 30S subunit (By similarity).[HAMAP-Rule:MF_00403_B] Cryo-EM studies suggest that S12 contacts the EF-Tu bound tRNA in the A-site during codon-recognition. This contact is most likely broken as the aminoacyl-tRNA moves into the peptidyl transferase center in the 50S subunit.[HAMAP-Rule:MF_00403_B] [[https://www.uniprot.org/uniprot/RRF_ECOLI RRF_ECOLI]] Responsible for the release of ribosomes from messenger RNA at the termination of protein biosynthesis. May increase the efficiency of translation by recycling ribosomes from one round of translation to another.[HAMAP-Rule:MF_00040]
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 16:45, 10 April 2020

OCA — Fri, 10 Apr 2020 16:45:12 GMT

←Older revision		Revision as of 16:45, 10 April 2020
Line 3:		Line 3:
	<SX load='1zn1' size='340' side='right' viewer='molstar' caption='[[1zn1]], [[Resolution\|resolution]] 14.10Å' scene=''>		<SX load='1zn1' size='340' side='right' viewer='molstar' caption='[[1zn1]], [[Resolution\|resolution]] 14.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1zn1]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZN1 OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1ZN1 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1zn1]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZN1 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=1ZN1 FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1p6g\|1p6g]], [[1p85\|1p85]], [[1ek8\|1ek8]], [[1zn0\|1zn0]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1p6g\|1p6g]], [[1p85\|1p85]], [[1ek8\|1ek8]], [[1zn0\|1zn0]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1zn1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1zn1 OCA], [http://pdbe.org/1zn1 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1zn1 RCSB], [http://www.ebi.ac.uk/pdbsum/1zn1 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1zn1 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=1zn1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1zn1 OCA], [http://pdbe.org/1zn1 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1zn1 RCSB], [http://www.ebi.ac.uk/pdbsum/1zn1 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1zn1 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==

OCA at 16:51, 6 March 2020

OCA — Fri, 06 Mar 2020 16:51:33 GMT

←Older revision		Revision as of 16:51, 6 March 2020
Line 1:		Line 1:

	==Coordinates of RRF fitted into Cryo-EM map of the 70S post-termination complex==		==Coordinates of RRF fitted into Cryo-EM map of the 70S post-termination complex==
-	<~~StructureSection~~ load='1zn1' size='340' side='right'caption='[[1zn1]], [[Resolution\|resolution]] 14.10Å' scene=''>	+	<SX load='1zn1' size='340' side='right' viewer='molstar' caption='[[1zn1]], [[Resolution\|resolution]] 14.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1zn1]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZN1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ZN1 FirstGlance]. <br>		<table><tr><td colspan='2'>[[1zn1]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZN1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ZN1 FirstGlance]. <br>
Line 35:		Line 35:
	<references/>		<references/>
	__TOC__		__TOC__
-	</~~StructureSection~~>	+	</SX>
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
	[[Category: Large Structures]]		[[Category: Large Structures]]

OCA at 11:47, 4 December 2019

OCA — Wed, 04 Dec 2019 11:47:57 GMT

←Older revision		Revision as of 11:47, 4 December 2019
Line 1:		Line 1:

	==Coordinates of RRF fitted into Cryo-EM map of the 70S post-termination complex==		==Coordinates of RRF fitted into Cryo-EM map of the 70S post-termination complex==
-	<StructureSection load='1zn1' size='340' side='right' caption='[[1zn1]], [[Resolution\|resolution]] 14.10Å' scene=''>	+	<StructureSection load='1zn1' size='340' side='right'caption='[[1zn1]], [[Resolution\|resolution]] 14.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1zn1]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZN1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ZN1 FirstGlance]. <br>		<table><tr><td colspan='2'>[[1zn1]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZN1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ZN1 FirstGlance]. <br>
Line 28:		Line 28:
	</div>		</div>
	<div class="pdbe-citations 1zn1" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 1zn1" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Ribosomal protein S12\|Ribosomal protein S12]]
		+	*[[Ribosome recycling factor\|Ribosome recycling factor]]
	== References ==		== References ==
	<references/>		<references/>
Line 33:		Line 37:
	</StructureSection>		</StructureSection>
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
		+	[[Category: Large Structures]]
	[[Category: Ehrenberg, M]]		[[Category: Ehrenberg, M]]
	[[Category: Frank, J]]		[[Category: Frank, J]]

OCA at 06:36, 31 January 2018

OCA — Wed, 31 Jan 2018 06:36:00 GMT

←Older revision		Revision as of 06:36, 31 January 2018
Line 1:		Line 1:
		+
	==Coordinates of RRF fitted into Cryo-EM map of the 70S post-termination complex==		==Coordinates of RRF fitted into Cryo-EM map of the 70S post-termination complex==
	<StructureSection load='1zn1' size='340' side='right' caption='[[1zn1]], [[Resolution\|resolution]] 14.10Å' scene=''>		<StructureSection load='1zn1' size='340' side='right' caption='[[1zn1]], [[Resolution\|resolution]] 14.10Å' scene=''>
Line 4:		Line 5:
	<table><tr><td colspan='2'>[[1zn1]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZN1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ZN1 FirstGlance]. <br>		<table><tr><td colspan='2'>[[1zn1]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZN1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ZN1 FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1p6g\|1p6g]], [[1p85\|1p85]], [[1ek8\|1ek8]], [[1zn0\|1zn0]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1p6g\|1p6g]], [[1p85\|1p85]], [[1ek8\|1ek8]], [[1zn0\|1zn0]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1zn1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1zn1 OCA], [http://pdbe.org/1zn1 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1zn1 RCSB], [http://www.ebi.ac.uk/pdbsum/1zn1 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1zn1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1zn1 OCA], [http://pdbe.org/1zn1 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1zn1 RCSB], [http://www.ebi.ac.uk/pdbsum/1zn1 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1zn1 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 12:		Line 13:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/zn/1zn1_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/zn/1zn1_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1zn1 ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 27:		Line 28:
	</div>		</div>
	<div class="pdbe-citations 1zn1" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 1zn1" style="background-color:#fffaf0;"></div>
-
-	==See Also==
-	*[[Ribosomal protein S12\|Ribosomal protein S12]]
-	*[[Ribosome recycling factor\|Ribosome recycling factor]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 00:15, 12 September 2015

OCA — Sat, 12 Sep 2015 00:15:19 GMT

←Older revision		Revision as of 00:15, 12 September 2015
Line 4:		Line 4:
	<table><tr><td colspan='2'>[[1zn1]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZN1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ZN1 FirstGlance]. <br>		<table><tr><td colspan='2'>[[1zn1]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZN1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ZN1 FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1p6g\|1p6g]], [[1p85\|1p85]], [[1ek8\|1ek8]], [[1zn0\|1zn0]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1p6g\|1p6g]], [[1p85\|1p85]], [[1ek8\|1ek8]], [[1zn0\|1zn0]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1zn1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1zn1 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1zn1 RCSB], [http://www.ebi.ac.uk/pdbsum/1zn1 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1zn1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1zn1 OCA], [http://pdbe.org/1zn1 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1zn1 RCSB], [http://www.ebi.ac.uk/pdbsum/1zn1 PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 26:		Line 26:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1zn1" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Ribosomal protein S12\|Ribosomal protein S12]]
		+	*[[Ribosome recycling factor\|Ribosome recycling factor]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 21:16, 25 December 2014

OCA — Thu, 25 Dec 2014 21:16:51 GMT

←Older revision		Revision as of 21:16, 25 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1zn1]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZN1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ZN1 FirstGlance]. <br>		<table><tr><td colspan='2'>[[1zn1]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZN1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ZN1 FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1p6g\|1p6g]], [[1p85\|1p85]], [[1ek8\|1ek8]], [[1zn0\|1zn0]]</td></tr>	+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1p6g\|1p6g]], [[1p85\|1p85]], [[1ek8\|1ek8]], [[1zn0\|1zn0]]</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1zn1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1zn1 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1zn1 RCSB], [http://www.ebi.ac.uk/pdbsum/1zn1 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1zn1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1zn1 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1zn1 RCSB], [http://www.ebi.ac.uk/pdbsum/1zn1 PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/RS12_ECOLI RS12_ECOLI]] With S4 and S5 plays an important role in translational accuracy.[HAMAP-Rule:MF_00403_B] Interacts with and stabilizes bases of the 16S rRNA that are involved in tRNA selection in the A site and with the mRNA backbone. Located at the interface of the 30S and 50S subunits, it traverses the body of the 30S subunit contacting proteins on the other side and probably holding the rRNA structure together. The combined cluster of proteins S8, S12 and S17 appears to hold together the shoulder and platform of the 30S subunit (By similarity).[HAMAP-Rule:MF_00403_B] Cryo-EM studies suggest that S12 contacts the EF-Tu bound tRNA in the A-site during codon-recognition. This contact is most likely broken as the aminoacyl-tRNA moves into the peptidyl transferase center in the 50S subunit.[HAMAP-Rule:MF_00403_B] [[http://www.uniprot.org/uniprot/RRF_ECOLI RRF_ECOLI]] Responsible for the release of ribosomes from messenger RNA at the termination of protein biosynthesis. May increase the efficiency of translation by recycling ribosomes from one round of translation to another.[HAMAP-Rule:MF_00040]
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	</StructureSection>		</StructureSection>
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
-	[[Category: Ehrenberg, M.]]	+	[[Category: Ehrenberg, M]]
-	[[Category: Frank, J.]]	+	[[Category: Frank, J]]
-	[[Category: Gao, N.]]	+	[[Category: Gao, N]]
-	[[Category: Gursky, R P.]]	+	[[Category: Gursky, R P]]
-	[[Category: Li, W.]]	+	[[Category: Li, W]]
-	[[Category: Sengupta, J.]]	+	[[Category: Sengupta, J]]
-	[[Category: Valle, M.]]	+	[[Category: Valle, M]]
-	[[Category: Zavialov, A V~~.]]~~	+	[[Category: Zavialov, A V]]
-	~~[[Category: 70~~]]	+
	[[Category: Biosynthetic-structural protein-rna complex]]		[[Category: Biosynthetic-structural protein-rna complex]]
	[[Category: Elongation factor g]]		[[Category: Elongation factor g]]
	[[Category: Post-termination complex]]		[[Category: Post-termination complex]]
	[[Category: Ribosome recycling factor]]		[[Category: Ribosome recycling factor]]

OCA at 04:51, 29 September 2014

OCA — Mon, 29 Sep 2014 04:51:50 GMT

←Older revision		Revision as of 04:51, 29 September 2014
Line 1:		Line 1:
-	[[~~Image:~~1zn1.~~png~~\|~~left~~\|200px]]	+	==Coordinates of RRF fitted into Cryo-EM map of the 70S post-termination complex==
		+	<StructureSection load='1zn1' size='340' side='right' caption='[[1zn1]], [[Resolution\|resolution]] 14.10Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1zn1]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZN1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ZN1 FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1p6g\|1p6g]], [[1p85\|1p85]], [[1ek8\|1ek8]], [[1zn0\|1zn0]]</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1zn1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1zn1 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1zn1 RCSB], [http://www.ebi.ac.uk/pdbsum/1zn1 PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/zn/1zn1_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Ribosome recycling, the disassembly of the posttermination complex after each round of protein synthesis, is an essential step in mRNA translation, but its mechanism has remained obscure. In eubacteria, recycling is catalyzed by RRF (ribosome recycling factor) and EF-G (elongation factor G). By using cryo-electron microscopy, we have obtained two density maps, one of the RRF bound posttermination complex and one of the 50S subunit bound with both EF-G and RRF. Comparing the two maps, we found domain I of RRF to be in the same orientation, while domain II in the EF-G-containing 50S subunit is extensively rotated (approximately 60 degrees) compared to its orientation in the 70S complex. Mapping the 50S conformation of RRF onto the 70S posttermination complex suggests that it can disrupt the intersubunit bridges B2a and B3, and thus effect a separation of the two subunits. These observations provide the structural basis for the mechanism by which the posttermination complex is split into subunits by the joint action of RRF and EF-G.

-	~~{{STRUCTURE_1zn1\| PDB=1zn1 \| SCENE= }}~~	+	Mechanism for the disassembly of the posttermination complex inferred from cryo-EM studies.,Gao N, Zavialov AV, Li W, Sengupta J, Valle M, Gursky RP, Ehrenberg M, Frank J Mol Cell. 2005 Jun 10;18(6):663-74. PMID:15949441<ref>PMID:15949441</ref>

-	~~===Coordinates of RRF fitted into Cryo-EM map~~ of the ~~70S post-termination complex===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_15949441}}~~	+	== References ==
-		+	<references/>
-	~~==About this Structure==~~	+	__TOC__
-	~~[[1zn1]] is a 4 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Escherichia_coli Escherichia coli]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=1ZN1 OCA].~~	+	</StructureSection>
-		+
-	==~~See Also==~~	+
-	*[[Ribosomal protein S12\|Ribosomal protein S12]]	+
-		+
-	~~==Reference~~==	+
-	<~~ref group="xtra">PMID:015949441<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
	[[Category: Ehrenberg, M.]]		[[Category: Ehrenberg, M.]]

OCA at 21:45, 20 October 2012

OCA — Sat, 20 Oct 2012 21:45:47 GMT

←Older revision		Revision as of 21:45, 20 October 2012
Line 8:		Line 8:

	==About this Structure==		==About this Structure==
-	[[1zn1]] is a 4 chain structure ~~of [[Ribosomal protein S12]]~~ with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZN1 OCA].	+	[[1zn1]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZN1 OCA].

	==See Also==		==See Also==