1zyw - Revision history

OCA at 08:15, 25 October 2023

OCA — Wed, 25 Oct 2023 08:15:55 GMT

←Older revision		Revision as of 08:15, 25 October 2023
Line 3:		Line 3:
	<StructureSection load='1zyw' size='340' side='right'caption='[[1zyw]], [[Resolution\|resolution]] 1.30Å' scene=''>		<StructureSection load='1zyw' size='340' side='right'caption='[[1zyw]], [[Resolution\|resolution]] 1.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1zyw]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Buthus_martensi Buthus martensi~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZYW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1ZYW FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1zyw]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mesobuthus_martensii Mesobuthus martensii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZYW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1ZYW FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>~~, ~~<scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.3Å</td></tr>
-	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"><~~div style~~='~~overflow~~: ~~auto; max-height: 3em;~~'>~~[[1zyv\|1zyv]]~~</~~div~~><~~/td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	~~<tr id~~='~~gene'><td class~~=~~"sblockLbl"><b>[[Gene\|Gene~~:~~]]</b~~></td>~~<td class="sblockDat">BMK M1 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=34649 Buthus martensi])~~</td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1zyw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1zyw OCA], [https://pdbe.org/1zyw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1zyw RCSB], [https://www.ebi.ac.uk/pdbsum/1zyw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1zyw ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1zyw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1zyw OCA], [https://pdbe.org/1zyw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1zyw RCSB], [https://www.ebi.ac.uk/pdbsum/1zyw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1zyw ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/~~SCX1_MESMA SCX1_MESMA~~]~~] This alpha-like toxin binds~~ voltage-~~dependently~~ sodium channels and ~~inhibits~~ the inactivation of the activated channels, thereby blocking neuronal transmission. This toxin is active against mammals and insects. Is active on Nav1.4/SCN4A and Nav1.5/SCN5A. ~~Acts as~~ a ~~cardiotoxin~~. Is 6-fold more toxic than BmK-M2.<ref>PMID:11322948</ref> <ref>PMID:12705833</ref>	+	[https://www.uniprot.org/uniprot/SCM1_MESMA SCM1_MESMA] Alpha toxins bind voltage-independently at site-3 of sodium channels (Nav) and inhibit the inactivation of the activated channels thereby blocking neuronal transmission. This toxin is active against both mammals and insects, and is classified as an alpha-like toxin. It is active on Nav1.2/SCN2A (EC(50)=139-252 nM), Nav1.3/SCN3A (EC(50)=565 nM), Nav1.4/SCN4A and Nav1.5/SCN5A (EC(50)=195-500 nM), Nav1.6/SCN8A (EC(50)=214 nM), and drosophila DmNav1 (EC(50)=30 nM) (PubMed:11322948, PubMed:12705833, PubMed:15677695, PubMed:19162162, PubMed:20678086). In mNav1.6/SCN8A, the toxin induces a large increase in both transient and persistent currents, which correlates with a prominent reduction in the fast component of inactivating current (PubMed:20678086). In rNav1.2/SCN2A and rNav1.3/SCN3A, toxin-increased currents is much smaller (PubMed:19162162, PubMed:20678086). Moreover, the toxin only accelerates the slow inactivation development and delay recovery of mNav1.6/SCN8A through binding to the channel in the open state (PubMed:20678086). Is 6-fold more toxic than BmK-M2. In vivo, intrahippocampal injection into rat induces epileptiform responses (PubMed:16229835). In addition, intraplantar injection into rat induces spontaneous nociception and hyperalgesia (PubMed:14554105).<ref>PMID:11322948</ref> <ref>PMID:12705833</ref> <ref>PMID:14554105</ref> <ref>PMID:15677695</ref> <ref>PMID:16229835</ref> <ref>PMID:19162162</ref> <ref>PMID:20678086</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Buthus martensi]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Bosmans, F]]	+	[[Category: Mesobuthus martensii]]
-	[[Category: Li, C]]	+	[[Category: Bosmans F]]
-	[[Category: Tytgat, J]]	+	[[Category: Li C]]
-	[[Category: Wang~~, D C~~]]	+	[[Category: Tytgat J]]
-	[[Category: Ye, X]]	+	[[Category: Wang DC]]
-	[[Category: Zhang, Y]]	+	[[Category: Ye X]]
-	~~[[Category: Bmk m1]]~~	+	[[Category: Zhang Y]]
-	~~[[Category: Mammal/insect selectivity]]~~	+
-	~~[[Category: Mutant]]~~	+
-	~~[[Category: Scorpion alpha-like toxin]]~~	+
-	~~[[Category: Toxin~~]]	+

OCA at 08:20, 27 January 2021

OCA — Wed, 27 Jan 2021 08:20:38 GMT

←Older revision		Revision as of 08:20, 27 January 2021
Line 1:		Line 1:

	==Crystal Structure Of Mutant K8DP9SR58KP60G Of Scorpion alpha-Like Neurotoxin Bmk M1 From Buthus Martensii Karsch==		==Crystal Structure Of Mutant K8DP9SR58KP60G Of Scorpion alpha-Like Neurotoxin Bmk M1 From Buthus Martensii Karsch==
-	<StructureSection load='1zyw' size='340' side='right' caption='[[1zyw]], [[Resolution\|resolution]] 1.30Å' scene=''>	+	<StructureSection load='1zyw' size='340' side='right'caption='[[1zyw]], [[Resolution\|resolution]] 1.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1zyw]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Buthus_martensi Buthus martensi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZYW OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1ZYW FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1zyw]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Buthus_martensi Buthus martensi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZYW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1ZYW FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1zyv\|1zyv]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1zyv\|1zyv]]</div></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">BMK M1 ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=34649 Buthus martensi])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">BMK M1 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=34649 Buthus martensi])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1zyw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1zyw OCA], [~~http~~://pdbe.org/1zyw PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1zyw RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1zyw PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1zyw ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1zyw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1zyw OCA], [https://pdbe.org/1zyw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1zyw RCSB], [https://www.ebi.ac.uk/pdbsum/1zyw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1zyw ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/SCX1_MESMA SCX1_MESMA]] This alpha-like toxin binds voltage-dependently sodium channels and inhibits the inactivation of the activated channels, thereby blocking neuronal transmission. This toxin is active against mammals and insects. Is active on Nav1.4/SCN4A and Nav1.5/SCN5A. Acts as a cardiotoxin. Is 6-fold more toxic than BmK-M2.<ref>PMID:11322948</ref> <ref>PMID:12705833</ref>	+	[[https://www.uniprot.org/uniprot/SCX1_MESMA SCX1_MESMA]] This alpha-like toxin binds voltage-dependently sodium channels and inhibits the inactivation of the activated channels, thereby blocking neuronal transmission. This toxin is active against mammals and insects. Is active on Nav1.4/SCN4A and Nav1.5/SCN5A. Acts as a cardiotoxin. Is 6-fold more toxic than BmK-M2.<ref>PMID:11322948</ref> <ref>PMID:12705833</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/zy/1zyw_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/zy/1zyw_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
Line 35:		Line 35:
	</StructureSection>		</StructureSection>
	[[Category: Buthus martensi]]		[[Category: Buthus martensi]]
		+	[[Category: Large Structures]]
	[[Category: Bosmans, F]]		[[Category: Bosmans, F]]
	[[Category: Li, C]]		[[Category: Li, C]]

OCA at 14:05, 12 October 2017

OCA — Thu, 12 Oct 2017 14:05:43 GMT

←Older revision		Revision as of 14:05, 12 October 2017
Line 1:		Line 1:
		+
	==Crystal Structure Of Mutant K8DP9SR58KP60G Of Scorpion alpha-Like Neurotoxin Bmk M1 From Buthus Martensii Karsch==		==Crystal Structure Of Mutant K8DP9SR58KP60G Of Scorpion alpha-Like Neurotoxin Bmk M1 From Buthus Martensii Karsch==
	<StructureSection load='1zyw' size='340' side='right' caption='[[1zyw]], [[Resolution\|resolution]] 1.30Å' scene=''>		<StructureSection load='1zyw' size='340' side='right' caption='[[1zyw]], [[Resolution\|resolution]] 1.30Å' scene=''>
Line 6:		Line 7:
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1zyv\|1zyv]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1zyv\|1zyv]]</td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">BMK M1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=34649 Buthus martensi])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">BMK M1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=34649 Buthus martensi])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1zyw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1zyw OCA], [http://pdbe.org/1zyw PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1zyw RCSB], [http://www.ebi.ac.uk/pdbsum/1zyw PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1zyw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1zyw OCA], [http://pdbe.org/1zyw PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1zyw RCSB], [http://www.ebi.ac.uk/pdbsum/1zyw PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1zyw ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 18:		Line 19:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1zyw ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 22:29, 11 September 2015

OCA — Fri, 11 Sep 2015 22:29:16 GMT

←Older revision		Revision as of 22:29, 11 September 2015
Line 2:		Line 2:
	<StructureSection load='1zyw' size='340' side='right' caption='[[1zyw]], [[Resolution\|resolution]] 1.30Å' scene=''>		<StructureSection load='1zyw' size='340' side='right' caption='[[1zyw]], [[Resolution\|resolution]] 1.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1zyw]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Mesobuthus_martensii Mesobuthus martensii~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZYW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ZYW FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1zyw]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Buthus_martensi Buthus martensi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZYW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ZYW FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1zyv\|1zyv]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1zyv\|1zyv]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">BMK M1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=34649 ~~Mesobuthus martensii~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">BMK M1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=34649 Buthus martensi])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1zyw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1zyw OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1zyw RCSB], [http://www.ebi.ac.uk/pdbsum/1zyw PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1zyw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1zyw OCA], [http://pdbe.org/1zyw PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1zyw RCSB], [http://www.ebi.ac.uk/pdbsum/1zyw PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 28:		Line 28:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1zyw" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Mesobuthus martensii~~]]	+	[[Category: Buthus martensi]]
	[[Category: Bosmans, F]]		[[Category: Bosmans, F]]
	[[Category: Li, C]]		[[Category: Li, C]]

OCA at 20:18, 25 December 2014

OCA — Thu, 25 Dec 2014 20:18:01 GMT

←Older revision		Revision as of 20:18, 25 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1zyw]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Mesobuthus_martensii Mesobuthus martensii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZYW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ZYW FirstGlance]. <br>		<table><tr><td colspan='2'>[[1zyw]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Mesobuthus_martensii Mesobuthus martensii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZYW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ZYW FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1zyv\|1zyv]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1zyv\|1zyv]]</td></tr>
-	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">BMK M1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=34649 Mesobuthus martensii])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">BMK M1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=34649 Mesobuthus martensii])</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1zyw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1zyw OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1zyw RCSB], [http://www.ebi.ac.uk/pdbsum/1zyw PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1zyw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1zyw OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1zyw RCSB], [http://www.ebi.ac.uk/pdbsum/1zyw PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/SCX1_MESMA SCX1_MESMA]] This alpha-like toxin binds voltage-dependently sodium channels and inhibits the inactivation of the activated channels, thereby blocking neuronal transmission. This toxin is active against mammals and insects. Is active on Nav1.4/SCN4A and Nav1.5/SCN5A. Acts as a cardiotoxin. Is 6-fold more toxic than BmK-M2.<ref>PMID:11322948</ref> <ref>PMID:12705833</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 31:		Line 33:
	</StructureSection>		</StructureSection>
	[[Category: Mesobuthus martensii]]		[[Category: Mesobuthus martensii]]
-	[[Category: Bosmans, F.]]	+	[[Category: Bosmans, F]]
-	[[Category: Li, C.]]	+	[[Category: Li, C]]
-	[[Category: Tytgat, J.]]	+	[[Category: Tytgat, J]]
-	[[Category: Wang, D C.]]	+	[[Category: Wang, D C]]
-	[[Category: Ye, X.]]	+	[[Category: Ye, X]]
-	[[Category: Zhang, Y.]]	+	[[Category: Zhang, Y]]
	[[Category: Bmk m1]]		[[Category: Bmk m1]]
	[[Category: Mammal/insect selectivity]]		[[Category: Mammal/insect selectivity]]

OCA at 02:03, 30 September 2014

OCA — Tue, 30 Sep 2014 02:03:58 GMT

←Older revision		Revision as of 02:03, 30 September 2014
Line 1:		Line 1:
-	[[~~Image:~~1zyw.~~png~~\|~~left~~\|200px]]	+	==Crystal Structure Of Mutant K8DP9SR58KP60G Of Scorpion alpha-Like Neurotoxin Bmk M1 From Buthus Martensii Karsch==
		+	<StructureSection load='1zyw' size='340' side='right' caption='[[1zyw]], [[Resolution\|resolution]] 1.30Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1zyw]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Mesobuthus_martensii Mesobuthus martensii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZYW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ZYW FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1zyv\|1zyv]]</td></tr>
		+	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">BMK M1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=34649 Mesobuthus martensii])</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1zyw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1zyw OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1zyw RCSB], [http://www.ebi.ac.uk/pdbsum/1zyw PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/zy/1zyw_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Scorpion alpha-like toxins are proteins that act on mammalian and insect voltage-gated Na+ channels. Therefore, these toxins constitute an excellent target for examining the foundations that underlie their target specificity. With this motive we dissected the role of six critical amino acids located in the five-residue reverse turn (RT) and C-tail (CT) of the scorpion alpha-like toxin BmK M1. These residues were individually substituted resulting in 11 mutants and were subjected to a bioassay on mice, an electrophysiological characterization on three cloned voltage-gated Na+ channels (Nav1.2, Nav1.5 and para), a CD analysis and X-ray crystallography. The results reveal two molecular sites, a couplet of residues (8-9) in the RT and a hydrophobic surface consisting of residues 57 and 59-61 in the CT, where the substitution with specific residues can redirect the alpha-like characteristics of BmK M1 to either total insect or much higher mammal specificity. Crystal structures reveal that the pharmacological ramification of these mutants is accompanied by the reshaping of the 3D structure surrounding position 8. Furthermore, our results also reveal that residues 57 and 59-61, located at the CT, enclose the critical residue 58 in order to form a hydrophobic "gasket". Mutants of BmK M1 that interrupt this hydrophobic surface significantly gain insect selectivity.

-	~~{{STRUCTURE_1zyw\| PDB=1zyw \| SCENE= }}~~	+	Structural basis for the voltage-gated Na+ channel selectivity of the scorpion alpha-like toxin BmK M1.,Ye X, Bosmans F, Li C, Zhang Y, Wang DC, Tytgat J J Mol Biol. 2005 Nov 4;353(4):788-803. Epub 2005 Sep 22. PMID:16209876<ref>PMID:16209876</ref>

-	~~===Crystal Structure Of Mutant K8DP9SR58KP60G Of Scorpion alpha-Like Neurotoxin Bmk M1~~ From ~~Buthus Martensii Karsch===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_16209876}}~~	+	== References ==
-		+	<references/>
-	~~==About this Structure==~~	+	__TOC__
-	~~[[1zyw]] is~~ a ~~1 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Mesobuthus_martensii Mesobuthus martensii]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=1ZYW OCA].~~	+	</StructureSection>
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:016209876<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Mesobuthus martensii]]		[[Category: Mesobuthus martensii]]
	[[Category: Bosmans, F.]]		[[Category: Bosmans, F.]]

OCA: Protected "1zyw" [edit=sysop:move=sysop]

OCA — Sun, 06 Jan 2013 11:17:25 GMT

Protected "1zyw" [edit=sysop:move=sysop]

←Older revision

Revision as of 11:17, 6 January 2013

OCA at 11:17, 6 January 2013

OCA — Sun, 06 Jan 2013 11:17:23 GMT

←Older revision		Revision as of 11:17, 6 January 2013
Line 1:		Line 1:
-	{{Seed}}
	[[Image:1zyw.png\|left\|200px]]		[[Image:1zyw.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_1zyw", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_1zyw\| PDB=1zyw \| SCENE= }}		{{STRUCTURE_1zyw\| PDB=1zyw \| SCENE= }}

	===Crystal Structure Of Mutant K8DP9SR58KP60G Of Scorpion alpha-Like Neurotoxin Bmk M1 From Buthus Martensii Karsch===		===Crystal Structure Of Mutant K8DP9SR58KP60G Of Scorpion alpha-Like Neurotoxin Bmk M1 From Buthus Martensii Karsch===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_16209876}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 16209876 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_16209876}}		{{ABSTRACT_PUBMED_16209876}}

	==About this Structure==		==About this Structure==
-	~~1ZYW~~ is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Mesobuthus_martensii Mesobuthus martensii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZYW OCA].	+	[[1zyw]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Mesobuthus_martensii Mesobuthus martensii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZYW OCA].

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~16209876~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:016209876</ref><references group="xtra"/>
	[[Category: Mesobuthus martensii]]		[[Category: Mesobuthus martensii]]
	[[Category: Bosmans, F.]]		[[Category: Bosmans, F.]]
Line 35:		Line 23:
	[[Category: Mutant]]		[[Category: Mutant]]
	[[Category: Scorpion alpha-like toxin]]		[[Category: Scorpion alpha-like toxin]]
-		+	[[Category: Toxin]]
-	~~''Page seeded by~~ [~~http~~:~~//oca.weizmann.ac.il/oca OCA~~ ] ~~on Wed Feb 18 05:56:48 2009''~~	+

OCA at 03:56, 18 February 2009

OCA — Wed, 18 Feb 2009 03:56:51 GMT

←Older revision		Revision as of 03:56, 18 February 2009
Line 20:		Line 20:

	==About this Structure==		==About this Structure==
-	1ZYW is a ~~[[Single protein]]~~ structure of sequence from [http://en.wikipedia.org/wiki/Mesobuthus_martensii Mesobuthus martensii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZYW OCA].	+	1ZYW is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Mesobuthus_martensii Mesobuthus martensii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZYW OCA].

	==Reference==		==Reference==
-	Structural basis for the voltage-gated Na+ channel selectivity of the scorpion alpha-like toxin BmK M1., Ye X, Bosmans F, Li C, Zhang Y, Wang DC, Tytgat J, J Mol Biol. 2005 Nov 4;353(4):788-803. Epub 2005 Sep 22. PMID:~~[http:~~//~~www.ncbi.nlm.nih.gov/pubmed/16209876 16209876]~~	+	<ref group="xtra">PMID:16209876</ref><references group="xtra"/>
	[[Category: Mesobuthus martensii]]		[[Category: Mesobuthus martensii]]
-	[[Category: Single protein]]
	[[Category: Bosmans, F.]]		[[Category: Bosmans, F.]]
	[[Category: Li, C.]]		[[Category: Li, C.]]
Line 37:		Line 36:
	[[Category: Scorpion alpha-like toxin]]		[[Category: Scorpion alpha-like toxin]]

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Tue Jul 29 12~~:41:~~11 2008~~''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Feb 18 05:56:48 2009''

OCA at 09:41, 29 July 2008

OCA — Tue, 29 Jul 2008 09:41:15 GMT

←Older revision		Revision as of 09:41, 29 July 2008
Line 1:		Line 1:
-	[[Image:1zyw.~~gif~~\|left\|200px]]	+	{{Seed}}
		+	[[Image:1zyw.png\|left\|200px]]

	<!--		<!--
Line 9:		Line 10:
	{{STRUCTURE_1zyw\| PDB=1zyw \| SCENE= }}		{{STRUCTURE_1zyw\| PDB=1zyw \| SCENE= }}

-	~~'''~~Crystal Structure Of Mutant K8DP9SR58KP60G Of Scorpion alpha-Like Neurotoxin Bmk M1 From Buthus Martensii Karsch~~'''~~	+	===Crystal Structure Of Mutant K8DP9SR58KP60G Of Scorpion alpha-Like Neurotoxin Bmk M1 From Buthus Martensii Karsch===


-	~~==Overview==~~	+	<!--
-	~~Scorpion alpha~~-~~like toxins are proteins that act on mammalian and insect voltage~~-~~gated Na+ channels. Therefore~~, ~~these toxins constitute an excellent target for examining~~ the ~~foundations that underlie their target specificity. With this motive we dissected~~ the ~~role of six critical amino acids located in the five-residue reverse turn~~ (~~RT) and C-tail (CT) of the scorpion alpha-like toxin BmK M1. These residues were individually substituted resulting in 11 mutants and were subjected to a bioassay~~ on ~~mice, an electrophysiological characterization on three cloned voltage-gated Na+ channels (Nav1~~.~~2, Nav1~~.~~5 and para~~)~~, a CD analysis and X-ray crystallography. The results reveal two molecular sites, a couplet of residues (8-9) in the RT and a hydrophobic surface consisting of residues 57 and 59-61 in the CT~~, where the substitution with specific residues can redirect the alpha-like characteristics of BmK M1 to either total insect or much higher mammal specificity. Crystal structures reveal that the pharmacological ramification of these mutants is ~~accompanied by~~ the ~~reshaping of the 3D structure surrounding position 8~~. ~~Furthermore, our results also reveal that residues 57 and 59~~-~~61, located at the CT, enclose the critical residue 58 in order to form a hydrophobic "gasket". Mutants of BmK M1 that interrupt this hydrophobic surface significantly gain insect selectivity.~~	+	The line below this paragraph, {{ABSTRACT_PUBMED_16209876}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 16209876 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_16209876}}

	==About this Structure==		==About this Structure==
Line 32:		Line 36:
	[[Category: Mutant]]		[[Category: Mutant]]
	[[Category: Scorpion alpha-like toxin]]		[[Category: Scorpion alpha-like toxin]]
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Sat May 3 18~~:15:15 2008''	+
		+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 12:41:11 2008''