2a7l - Revision history

OCA at 07:20, 23 August 2023

OCA — Wed, 23 Aug 2023 07:20:05 GMT

←Older revision		Revision as of 07:20, 23 August 2023
Line 3:		Line 3:
	<StructureSection load='2a7l' size='340' side='right'caption='[[2a7l]], [[Resolution\|resolution]] 1.82Å' scene=''>		<StructureSection load='2a7l' size='340' side='right'caption='[[2a7l]], [[Resolution\|resolution]] 1.82Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2a7l]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Human Human~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2A7L OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2A7L FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2a7l]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2A7L OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2A7L FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=NA:SODIUM+ION'>NA</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.82Å</td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat"~~>FLJ11011 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&~~id=~~9606 HUMAN])</td~~><~~/tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
-	~~<tr id~~='~~activity'><td class~~=~~"sblockLbl"><b>Activity~~:~~</b></td><td class="sblockDat"><span class='plainlinks~~'>~~[https://en.wikipedia.org/wiki/Ubiquitin--protein_ligase Ubiquitin--protein ligase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.3.2.19 6.3.2.19]~~ </~~span~~></td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2a7l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2a7l OCA], [https://pdbe.org/2a7l PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2a7l RCSB], [https://www.ebi.ac.uk/pdbsum/2a7l PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2a7l ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2a7l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2a7l OCA], [https://pdbe.org/2a7l PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2a7l RCSB], [https://www.ebi.ac.uk/pdbsum/2a7l PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2a7l ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/UBE2W_HUMAN UBE2W_HUMAN]] Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. Catalyzes monoubiquitination. Involved in degradation of misfolded chaperone substrates by mediating monoubiquitination of STUB1/CHIP, leading to recruitment of ATXN3 to monoubiquitinated STUB1/CHIP, and restriction of the length of ubiquitin chain attached to STUB1/CHIP substrates by ATXN3. After UV irradiation, but not after mitomycin-C (MMC) treatment, acts as a specific E2 ubiquitin-conjugating enzyme for the Fanconi anemia complex by associating with E3 ubiquitin-protein ligase FANCL and catalyzing monoubiquitination of FANCD2, a key step in the DNA damage pathway. In vitro catalyzes 'Lys-11'-linked polyubiquitination. Transfers ubiquitin in complex with RING/U-box type E3s that do not have active site cysteine residues to form thioester bonds with ubiquitin, and preferentially ubiquitinates the N-terminus of substrates, such as ATXN3, STUB1 and SUMO2.<ref>PMID:19111657</ref> <ref>PMID:20061386</ref> <ref>PMID:21229326</ref> <ref>PMID:23560854</ref> <ref>PMID:23696636</ref>	+	[https://www.uniprot.org/uniprot/UBE2W_HUMAN UBE2W_HUMAN] Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. Catalyzes monoubiquitination. Involved in degradation of misfolded chaperone substrates by mediating monoubiquitination of STUB1/CHIP, leading to recruitment of ATXN3 to monoubiquitinated STUB1/CHIP, and restriction of the length of ubiquitin chain attached to STUB1/CHIP substrates by ATXN3. After UV irradiation, but not after mitomycin-C (MMC) treatment, acts as a specific E2 ubiquitin-conjugating enzyme for the Fanconi anemia complex by associating with E3 ubiquitin-protein ligase FANCL and catalyzing monoubiquitination of FANCD2, a key step in the DNA damage pathway. In vitro catalyzes 'Lys-11'-linked polyubiquitination. Transfers ubiquitin in complex with RING/U-box type E3s that do not have active site cysteine residues to form thioester bonds with ubiquitin, and preferentially ubiquitinates the N-terminus of substrates, such as ATXN3, STUB1 and SUMO2.<ref>PMID:19111657</ref> <ref>PMID:20061386</ref> <ref>PMID:21229326</ref> <ref>PMID:23560854</ref> <ref>PMID:23696636</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 34:		Line 33:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Human~~]]	+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	~~[[Category: Ubiquitin--protein ligase]]~~	+	[[Category: Arrowsmith C]]
-	[[Category: Arrowsmith, C]]	+	[[Category: Avvakumov GV]]
-	[[Category: Avvakumov~~, G V~~]]	+	[[Category: Bochkarev A]]
-	[[Category: Bochkarev, A]]	+	[[Category: Dhe-Paganon S]]
-	[[Category: Dhe-Paganon, S]]	+	[[Category: Edwards A]]
-	[[Category: Edwards, A]]	+	[[Category: Mackenzie F]]
-	[[Category: Mackenzie, F]]	+	[[Category: Newman EM]]
-	[[Category: Newman~~, E M]]~~	+	[[Category: Sundstrom M]]
-	~~[[Category: Structural genomic~~]]	+	[[Category: Walker JR]]
-	[[Category: Sundstrom, M]]	+	[[Category: Weigelt J]]
-	[[Category: Walker~~, J R~~]]	+	[[Category: Xue S]]
-	[[Category: Weigelt, J]]	+
-	[[Category: Xue, S]]	+
-	~~[[Category: Ligase]]~~	+
-	~~[[Category: Ubiquitin]]~~	+
-	~~[[Category: Ubiquitin- conjugating enzyme~~]]	+

OCA at 08:26, 27 January 2021

OCA — Wed, 27 Jan 2021 08:26:42 GMT

←Older revision		Revision as of 08:26, 27 January 2021
Line 1:		Line 1:

	==Structure of the human hypothetical ubiquitin-conjugating enzyme, LOC55284==		==Structure of the human hypothetical ubiquitin-conjugating enzyme, LOC55284==
-	<StructureSection load='2a7l' size='340' side='right' caption='[[2a7l]], [[Resolution\|resolution]] 1.82Å' scene=''>	+	<StructureSection load='2a7l' size='340' side='right'caption='[[2a7l]], [[Resolution\|resolution]] 1.82Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2a7l]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2A7L OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2A7L FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2a7l]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2A7L OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2A7L FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">FLJ11011 ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">FLJ11011 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Ubiquitin--protein_ligase Ubiquitin--protein ligase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.3.2.19 6.3.2.19] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Ubiquitin--protein_ligase Ubiquitin--protein ligase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.3.2.19 6.3.2.19] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2a7l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2a7l OCA], [~~http~~://pdbe.org/2a7l PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2a7l RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2a7l PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2a7l ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2a7l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2a7l OCA], [https://pdbe.org/2a7l PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2a7l RCSB], [https://www.ebi.ac.uk/pdbsum/2a7l PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2a7l ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/UBE2W_HUMAN UBE2W_HUMAN]] Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. Catalyzes monoubiquitination. Involved in degradation of misfolded chaperone substrates by mediating monoubiquitination of STUB1/CHIP, leading to recruitment of ATXN3 to monoubiquitinated STUB1/CHIP, and restriction of the length of ubiquitin chain attached to STUB1/CHIP substrates by ATXN3. After UV irradiation, but not after mitomycin-C (MMC) treatment, acts as a specific E2 ubiquitin-conjugating enzyme for the Fanconi anemia complex by associating with E3 ubiquitin-protein ligase FANCL and catalyzing monoubiquitination of FANCD2, a key step in the DNA damage pathway. In vitro catalyzes 'Lys-11'-linked polyubiquitination. Transfers ubiquitin in complex with RING/U-box type E3s that do not have active site cysteine residues to form thioester bonds with ubiquitin, and preferentially ubiquitinates the N-terminus of substrates, such as ATXN3, STUB1 and SUMO2.<ref>PMID:19111657</ref> <ref>PMID:20061386</ref> <ref>PMID:21229326</ref> <ref>PMID:23560854</ref> <ref>PMID:23696636</ref>	+	[[https://www.uniprot.org/uniprot/UBE2W_HUMAN UBE2W_HUMAN]] Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. Catalyzes monoubiquitination. Involved in degradation of misfolded chaperone substrates by mediating monoubiquitination of STUB1/CHIP, leading to recruitment of ATXN3 to monoubiquitinated STUB1/CHIP, and restriction of the length of ubiquitin chain attached to STUB1/CHIP substrates by ATXN3. After UV irradiation, but not after mitomycin-C (MMC) treatment, acts as a specific E2 ubiquitin-conjugating enzyme for the Fanconi anemia complex by associating with E3 ubiquitin-protein ligase FANCL and catalyzing monoubiquitination of FANCD2, a key step in the DNA damage pathway. In vitro catalyzes 'Lys-11'-linked polyubiquitination. Transfers ubiquitin in complex with RING/U-box type E3s that do not have active site cysteine residues to form thioester bonds with ubiquitin, and preferentially ubiquitinates the N-terminus of substrates, such as ATXN3, STUB1 and SUMO2.<ref>PMID:19111657</ref> <ref>PMID:20061386</ref> <ref>PMID:21229326</ref> <ref>PMID:23560854</ref> <ref>PMID:23696636</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 35:		Line 35:
	</StructureSection>		</StructureSection>
	[[Category: Human]]		[[Category: Human]]
		+	[[Category: Large Structures]]
	[[Category: Ubiquitin--protein ligase]]		[[Category: Ubiquitin--protein ligase]]
	[[Category: Arrowsmith, C]]		[[Category: Arrowsmith, C]]

OCA at 07:29, 9 May 2018

OCA — Wed, 09 May 2018 07:29:28 GMT

←Older revision		Revision as of 07:29, 9 May 2018
Line 1:		Line 1:
		+
	==Structure of the human hypothetical ubiquitin-conjugating enzyme, LOC55284==		==Structure of the human hypothetical ubiquitin-conjugating enzyme, LOC55284==
	<StructureSection load='2a7l' size='340' side='right' caption='[[2a7l]], [[Resolution\|resolution]] 1.82Å' scene=''>		<StructureSection load='2a7l' size='340' side='right' caption='[[2a7l]], [[Resolution\|resolution]] 1.82Å' scene=''>
Line 6:		Line 7:
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">FLJ11011 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">FLJ11011 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Ubiquitin--protein_ligase Ubiquitin--protein ligase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.3.2.19 6.3.2.19] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Ubiquitin--protein_ligase Ubiquitin--protein ligase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.3.2.19 6.3.2.19] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2a7l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2a7l OCA], [http://pdbe.org/2a7l PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2a7l RCSB], [http://www.ebi.ac.uk/pdbsum/2a7l PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2a7l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2a7l OCA], [http://pdbe.org/2a7l PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2a7l RCSB], [http://www.ebi.ac.uk/pdbsum/2a7l PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2a7l ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 14:		Line 15:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/a7/2a7l_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/a7/2a7l_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2a7l ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 22:43, 11 September 2015

OCA — Fri, 11 Sep 2015 22:43:33 GMT

←Older revision		Revision as of 22:43, 11 September 2015
Line 2:		Line 2:
	<StructureSection load='2a7l' size='340' side='right' caption='[[2a7l]], [[Resolution\|resolution]] 1.82Å' scene=''>		<StructureSection load='2a7l' size='340' side='right' caption='[[2a7l]], [[Resolution\|resolution]] 1.82Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2a7l]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Homo_sapiens Homo sapiens~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2A7L OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2A7L FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2a7l]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2A7L OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2A7L FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">FLJ11011 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 ~~Homo sapiens~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">FLJ11011 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Ubiquitin--protein_ligase Ubiquitin--protein ligase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.3.2.19 6.3.2.19] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Ubiquitin--protein_ligase Ubiquitin--protein ligase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.3.2.19 6.3.2.19] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2a7l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2a7l OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2a7l RCSB], [http://www.ebi.ac.uk/pdbsum/2a7l PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2a7l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2a7l OCA], [http://pdbe.org/2a7l PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2a7l RCSB], [http://www.ebi.ac.uk/pdbsum/2a7l PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 28:		Line 28:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 2a7l" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Homo sapiens~~]]	+	[[Category: Human]]
	[[Category: Ubiquitin--protein ligase]]		[[Category: Ubiquitin--protein ligase]]
	[[Category: Arrowsmith, C]]		[[Category: Arrowsmith, C]]

OCA at 20:25, 25 December 2014

OCA — Thu, 25 Dec 2014 20:25:44 GMT

←Older revision		Revision as of 20:25, 25 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2a7l]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2A7L OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2A7L FirstGlance]. <br>		<table><tr><td colspan='2'>[[2a7l]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2A7L OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2A7L FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">FLJ11011 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">FLJ11011 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])</td></tr>
-	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Ubiquitin--protein_ligase Ubiquitin--protein ligase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.3.2.19 6.3.2.19] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Ubiquitin--protein_ligase Ubiquitin--protein ligase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.3.2.19 6.3.2.19] </span></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2a7l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2a7l OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2a7l RCSB], [http://www.ebi.ac.uk/pdbsum/2a7l PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2a7l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2a7l OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2a7l RCSB], [http://www.ebi.ac.uk/pdbsum/2a7l PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/UBE2W_HUMAN UBE2W_HUMAN]] Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. Catalyzes monoubiquitination. Involved in degradation of misfolded chaperone substrates by mediating monoubiquitination of STUB1/CHIP, leading to recruitment of ATXN3 to monoubiquitinated STUB1/CHIP, and restriction of the length of ubiquitin chain attached to STUB1/CHIP substrates by ATXN3. After UV irradiation, but not after mitomycin-C (MMC) treatment, acts as a specific E2 ubiquitin-conjugating enzyme for the Fanconi anemia complex by associating with E3 ubiquitin-protein ligase FANCL and catalyzing monoubiquitination of FANCD2, a key step in the DNA damage pathway. In vitro catalyzes 'Lys-11'-linked polyubiquitination. Transfers ubiquitin in complex with RING/U-box type E3s that do not have active site cysteine residues to form thioester bonds with ubiquitin, and preferentially ubiquitinates the N-terminus of substrates, such as ATXN3, STUB1 and SUMO2.<ref>PMID:19111657</ref> <ref>PMID:20061386</ref> <ref>PMID:21229326</ref> <ref>PMID:23560854</ref> <ref>PMID:23696636</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 32:		Line 34:
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
	[[Category: Ubiquitin--protein ligase]]		[[Category: Ubiquitin--protein ligase]]
-	[[Category: Arrowsmith, C.]]	+	[[Category: Arrowsmith, C]]
-	[[Category: Avvakumov, G V.]]	+	[[Category: Avvakumov, G V]]
-	[[Category: Bochkarev, A.]]	+	[[Category: Bochkarev, A]]
-	[[Category: Dhe-Paganon, S.]]	+	[[Category: Dhe-Paganon, S]]
-	[[Category: Edwards, A.]]	+	[[Category: Edwards, A]]
-	[[Category: Mackenzie, F.]]	+	[[Category: Mackenzie, F]]
-	[[Category: Newman, E M.]]	+	[[Category: Newman, E M]]
-	[[Category: ~~SGC,~~ Structural ~~Genomics Consortium.~~]]	+	[[Category: Structural genomic]]
-	[[Category: Sundstrom, M.]]	+	[[Category: Sundstrom, M]]
-	[[Category: Walker, J R.]]	+	[[Category: Walker, J R]]
-	[[Category: Weigelt, J.]]	+	[[Category: Weigelt, J]]
-	[[Category: Xue, S.]]	+	[[Category: Xue, S]]
	[[Category: Ligase]]		[[Category: Ligase]]
-	[[Category: Structural genomics consortium]]
	[[Category: Ubiquitin]]		[[Category: Ubiquitin]]
	[[Category: Ubiquitin- conjugating enzyme]]		[[Category: Ubiquitin- conjugating enzyme]]

OCA at 03:49, 29 September 2014

OCA — Mon, 29 Sep 2014 03:49:05 GMT

←Older revision		Revision as of 03:49, 29 September 2014
Line 1:		Line 1:
-	[[~~Image:~~2a7l.~~png~~\|~~left~~\|200px]]	+	==Structure of the human hypothetical ubiquitin-conjugating enzyme, LOC55284==
		+	<StructureSection load='2a7l' size='340' side='right' caption='[[2a7l]], [[Resolution\|resolution]] 1.82Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[2a7l]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2A7L OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2A7L FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">FLJ11011 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])</td></tr>
		+	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Ubiquitin--protein_ligase Ubiquitin--protein ligase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.3.2.19 6.3.2.19] </span></td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2a7l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2a7l OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2a7l RCSB], [http://www.ebi.ac.uk/pdbsum/2a7l PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/a7/2a7l_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Here we describe a systematic structure-function analysis of the human ubiquitin (Ub) E2 conjugating proteins, consisting of the determination of 15 new high-resolution three-dimensional structures of E2 catalytic domains, and autoubiquitylation assays for 26 Ub-loading E2s screened against a panel of nine different HECT (homologous to E6-AP carboxyl terminus) E3 ligase domains. Integration of our structural and biochemical data revealed several E2 surface properties associated with Ub chain building activity; (1) net positive or neutral E2 charge, (2) an "acidic trough" located near the catalytic Cys, surrounded by an extensive basic region, and (3) similarity to the previously described HECT binding signature in UBE2L3 (UbcH7). Mass spectrometry was used to characterize the autoubiquitylation products of a number of functional E2-HECT pairs, and demonstrated that HECT domains from different subfamilies catalyze the formation of very different types of Ub chains, largely independent of the E2 in the reaction. Our data set represents the first comprehensive analysis of E2-HECT E3 interactions, and thus provides a framework for better understanding the molecular mechanisms of ubiquitylation.

-	~~{{STRUCTURE_2a7l\| PDB=2a7l \| SCENE= }}~~	+	A human ubiquitin conjugating enzyme (E2)-HECT E3 ligase structure-function screen.,Sheng Y, Hong JH, Doherty R, Srikumar T, Shloush J, Avvakumov GV, Walker JR, Xue S, Neculai D, Wan JW, Kim SK, Arrowsmith CH, Raught B, Dhe-Paganon S Mol Cell Proteomics. 2012 Aug;11(8):329-41. Epub 2012 Apr 10. PMID:22496338<ref>PMID:22496338</ref>

-	~~===Structure~~ of the ~~human hypothetical ubiquitin-conjugating enzyme, LOC55284===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_22496338}}~~	+	== References ==
-		+	<references/>
-	==~~About this Structure~~==	+	__TOC__
-	~~[[2a7l]] is a 2 chain structure with sequence from [http:~~//~~en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2A7L OCA].~~	+	</StructureSection>
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
	[[Category: Ubiquitin--protein ligase]]		[[Category: Ubiquitin--protein ligase]]

OCA: Protected "2a7l" [edit=sysop:move=sysop]

OCA — Wed, 28 Nov 2012 14:32:17 GMT

Protected "2a7l" [edit=sysop:move=sysop]

←Older revision

Revision as of 14:32, 28 November 2012

OCA at 14:32, 28 November 2012

OCA — Wed, 28 Nov 2012 14:32:15 GMT

←Older revision		Revision as of 14:32, 28 November 2012
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-	{{Seed}}
	[[Image:2a7l.png\|left\|200px]]		[[Image:2a7l.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_2a7l", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_2a7l\| PDB=2a7l \| SCENE= }}		{{STRUCTURE_2a7l\| PDB=2a7l \| SCENE= }}

	===Structure of the human hypothetical ubiquitin-conjugating enzyme, LOC55284===		===Structure of the human hypothetical ubiquitin-conjugating enzyme, LOC55284===

		+	{{ABSTRACT_PUBMED_22496338}}

	==About this Structure==		==About this Structure==
-	~~2A7L~~ is a 2 ~~chains~~ structure ~~of sequences~~ from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2A7L OCA].	+	[[2a7l]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2A7L OCA].
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
	[[Category: Ubiquitin--protein ligase]]		[[Category: Ubiquitin--protein ligase]]
Line 33:		Line 27:
	[[Category: Ubiquitin]]		[[Category: Ubiquitin]]
	[[Category: Ubiquitin- conjugating enzyme]]		[[Category: Ubiquitin- conjugating enzyme]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Feb 18 06:11:12 2009''

OCA at 04:11, 18 February 2009

OCA — Wed, 18 Feb 2009 04:11:13 GMT

←Older revision		Revision as of 04:11, 18 February 2009
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-	[[Image:2a7l.~~gif~~\|left\|200px]]	+	{{Seed}}
		+	[[Image:2a7l.png\|left\|200px]]

	<!--		<!--
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	{{STRUCTURE_2a7l\| PDB=2a7l \| SCENE= }}		{{STRUCTURE_2a7l\| PDB=2a7l \| SCENE= }}

-	~~'''~~Structure of the human hypothetical ubiquitin-conjugating enzyme, LOC55284~~'''~~	+	===Structure of the human hypothetical ubiquitin-conjugating enzyme, LOC55284===


	==About this Structure==		==About this Structure==
-	2A7L is a ~~[[Single protein]]~~ structure of ~~sequence~~ from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2A7L OCA].	+	2A7L is a 2 chains structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2A7L OCA].
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
-	[[Category: Single protein]]
	[[Category: Ubiquitin--protein ligase]]		[[Category: Ubiquitin--protein ligase]]
	[[Category: Arrowsmith, C.]]		[[Category: Arrowsmith, C.]]
Line 33:		Line 33:
	[[Category: Ubiquitin]]		[[Category: Ubiquitin]]
	[[Category: Ubiquitin- conjugating enzyme]]		[[Category: Ubiquitin- conjugating enzyme]]
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Sat May 3~~ 18:42:~~35 2008~~''	+
		+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Feb 18 06:11:12 2009''

OCA at 15:42, 3 May 2008

OCA — Sat, 03 May 2008 15:42:35 GMT

←Older revision		Revision as of 15:42, 3 May 2008
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	[[Image:2a7l.gif\|left\|200px]]		[[Image:2a7l.gif\|left\|200px]]

-	~~{{Structure~~	+	<!--
-	~~\|PDB= 2a7l \|SIZE=350\|CAPTION= <scene name='initialview01'>2a7l</scene>~~, ~~resolution 1~~.~~82Å~~	+	The line below this paragraph, containing "STRUCTURE_2a7l", creates the "Structure Box" on the page.
-	~~\|SITE=~~	+	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	~~\|LIGAND= <~~scene ~~name='pdbligand=NA:SODIUM+ION'>NA</scene>~~	+	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	~~\|ACTIVITY= <span class='plainlinks'>[http://en~~.~~wikipedia.org/wiki/Ubiquitin~~--~~protein_ligase Ubiquitin--protein ligase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.3.2.19 6.3.2.19] </span~~>	+	or leave the SCENE parameter empty for the default display.
-	\|~~GENE~~= ~~FLJ11011 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])~~	+	-->
-	\|~~DOMAIN~~=	+	{{STRUCTURE_2a7l\| PDB=2a7l \| SCENE= }}
-	~~\|RELATEDENTRY=~~	+
-	\|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2a7l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2a7l OCA], [http://www.ebi.ac.uk/pdbsum/2a7l PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2a7l RCSB]</span>	+
-	}}	+

	'''Structure of the human hypothetical ubiquitin-conjugating enzyme, LOC55284'''		'''Structure of the human hypothetical ubiquitin-conjugating enzyme, LOC55284'''
Line 32:		Line 29:
	[[Category: Weigelt, J.]]		[[Category: Weigelt, J.]]
	[[Category: Xue, S.]]		[[Category: Xue, S.]]
-	[[Category: ~~(sgc)~~]]	+	[[Category: Ligase]]
-	[[Category: ~~ligase]]~~	+	[[Category: Structural genomics consortium]]
-	~~[[Category: structural~~ genomics consortium]]	+	[[Category: Ubiquitin]]
-	[[Category: ~~ubiquitin~~]]	+	[[Category: Ubiquitin- conjugating enzyme]]
-	[[Category: ~~ubiquitin~~- conjugating enzyme]]	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 18:42:35 2008''
-		+
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Mon Mar 31 01~~:48:54 2008''	+