2ab7 - Revision history

OCA at 16:31, 13 December 2023

OCA — Wed, 13 Dec 2023 16:31:59 GMT

←Older revision		Revision as of 16:31, 13 December 2023
Line 1:		Line 1:

	==Solution structures and characterization of HIV RRE IIB RNA targeting zinc finger proteins==		==Solution structures and characterization of HIV RRE IIB RNA targeting zinc finger proteins==
-	<StructureSection load='2ab7' size='340' side='right'caption='[[2ab7~~]], [[NMR_Ensembles_of_Models \| 1 NMR models~~]]' scene=''>	+	<StructureSection load='2ab7' size='340' side='right'caption='[[2ab7]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2ab7]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~"bacillus_coli"_migula_1895 "bacillus~~ coli~~" migula 1895~~]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AB7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2AB7 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2ab7]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AB7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2AB7 FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
-	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"><~~div style~~='~~overflow~~: ~~auto; max-height: 3em;~~'>~~[[2ab3\|2ab3]]~~</~~div~~></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ab7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ab7 OCA], [https://pdbe.org/2ab7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ab7 RCSB], [https://www.ebi.ac.uk/pdbsum/2ab7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ab7 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ab7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ab7 OCA], [https://pdbe.org/2ab7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ab7 RCSB], [https://www.ebi.ac.uk/pdbsum/2ab7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ab7 ProSAT]</span></td></tr>
	</table>		</table>
Line 21:		Line 21:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Bacillus~~ coli ~~migula 1895~~]]	+	[[Category: Escherichia coli]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Darby~~, M K~~]]	+	[[Category: Darby MK]]
-	[[Category: Germann~~, M W~~]]	+	[[Category: Germann MW]]
-	[[Category: Mishra~~, S H~~]]	+	[[Category: Mishra SH]]
-	[[Category: Shelley~~, C M]]~~	+	[[Category: Shelley CM]]
-	~~[[Category: Beta beta alpha]]~~	+
-	~~[[Category: Rna binding protein]]~~	+
-	~~[[Category: Rreiib-tr]]~~	+
-	~~[[Category: Zinc finger protein~~]]	+

OCA at 14:38, 17 November 2021

OCA — Wed, 17 Nov 2021 14:38:40 GMT

←Older revision		Revision as of 14:38, 17 November 2021
Line 3:		Line 3:
	<StructureSection load='2ab7' size='340' side='right'caption='[[2ab7]], [[NMR_Ensembles_of_Models \| 1 NMR models]]' scene=''>		<StructureSection load='2ab7' size='340' side='right'caption='[[2ab7]], [[NMR_Ensembles_of_Models \| 1 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2ab7]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AB7 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2AB7 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2ab7]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AB7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2AB7 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2ab3\|2ab3]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2ab3\|2ab3]]</div></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2ab7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ab7 OCA], [~~http~~://pdbe.org/2ab7 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2ab7 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2ab7 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2ab7 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ab7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ab7 OCA], [https://pdbe.org/2ab7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ab7 RCSB], [https://www.ebi.ac.uk/pdbsum/2ab7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ab7 ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 05:26, 13 February 2020

OCA — Thu, 13 Feb 2020 05:26:48 GMT

←Older revision		Revision as of 05:26, 13 February 2020
Line 1:		Line 1:

	==Solution structures and characterization of HIV RRE IIB RNA targeting zinc finger proteins==		==Solution structures and characterization of HIV RRE IIB RNA targeting zinc finger proteins==
-	<StructureSection load='2ab7' size='340' side='right' caption='[[2ab7]], [[NMR_Ensembles_of_Models \| 1 NMR models]]' scene=''>	+	<StructureSection load='2ab7' size='340' side='right'caption='[[2ab7]], [[NMR_Ensembles_of_Models \| 1 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2ab7]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AB7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2AB7 FirstGlance]. <br>		<table><tr><td colspan='2'>[[2ab7]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AB7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2AB7 FirstGlance]. <br>
Line 22:		Line 22:
	</StructureSection>		</StructureSection>
	[[Category: Bacillus coli migula 1895]]		[[Category: Bacillus coli migula 1895]]
		+	[[Category: Large Structures]]
	[[Category: Darby, M K]]		[[Category: Darby, M K]]
	[[Category: Germann, M W]]		[[Category: Germann, M W]]

OCA at 07:19, 9 May 2018

OCA — Wed, 09 May 2018 07:19:24 GMT

←Older revision		Revision as of 07:19, 9 May 2018
Line 1:		Line 1:
		+
	==Solution structures and characterization of HIV RRE IIB RNA targeting zinc finger proteins==		==Solution structures and characterization of HIV RRE IIB RNA targeting zinc finger proteins==
	<StructureSection load='2ab7' size='340' side='right' caption='[[2ab7]], [[NMR_Ensembles_of_Models \| 1 NMR models]]' scene=''>		<StructureSection load='2ab7' size='340' side='right' caption='[[2ab7]], [[NMR_Ensembles_of_Models \| 1 NMR models]]' scene=''>
Line 5:		Line 6:
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2ab3\|2ab3]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2ab3\|2ab3]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ab7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ab7 OCA], [http://pdbe.org/2ab7 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2ab7 RCSB], [http://www.ebi.ac.uk/pdbsum/2ab7 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ab7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ab7 OCA], [http://pdbe.org/2ab7 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2ab7 RCSB], [http://www.ebi.ac.uk/pdbsum/2ab7 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2ab7 ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 02:01, 11 September 2015

OCA — Fri, 11 Sep 2015 02:01:28 GMT

←Older revision		Revision as of 02:01, 11 September 2015
Line 2:		Line 2:
	<StructureSection load='2ab7' size='340' side='right' caption='[[2ab7]], [[NMR_Ensembles_of_Models \| 1 NMR models]]' scene=''>		<StructureSection load='2ab7' size='340' side='right' caption='[[2ab7]], [[NMR_Ensembles_of_Models \| 1 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2ab7]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Escherichia_coli Escherichia~~ coli]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AB7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2AB7 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2ab7]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AB7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2AB7 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2ab3\|2ab3]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2ab3\|2ab3]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ab7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ab7 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2ab7 RCSB], [http://www.ebi.ac.uk/pdbsum/2ab7 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ab7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ab7 OCA], [http://pdbe.org/2ab7 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2ab7 RCSB], [http://www.ebi.ac.uk/pdbsum/2ab7 PDBsum]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 15:		Line 15:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 2ab7" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Escherichia~~ coli]]	+	[[Category: Bacillus coli migula 1895]]
	[[Category: Darby, M K]]		[[Category: Darby, M K]]
	[[Category: Germann, M W]]		[[Category: Germann, M W]]

OCA at 05:57, 22 December 2014

OCA — Mon, 22 Dec 2014 05:57:12 GMT

←Older revision		Revision as of 05:57, 22 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2ab7]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AB7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2AB7 FirstGlance]. <br>		<table><tr><td colspan='2'>[[2ab7]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AB7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2AB7 FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2ab3\|2ab3]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2ab3\|2ab3]]</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ab7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ab7 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2ab7 RCSB], [http://www.ebi.ac.uk/pdbsum/2ab7 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ab7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ab7 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2ab7 RCSB], [http://www.ebi.ac.uk/pdbsum/2ab7 PDBsum]</span></td></tr>
-	<table>	+	</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 20:		Line 20:
	</StructureSection>		</StructureSection>
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
-	[[Category: Darby, M K.]]	+	[[Category: Darby, M K]]
-	[[Category: Germann, M W.]]	+	[[Category: Germann, M W]]
-	[[Category: Mishra, S H.]]	+	[[Category: Mishra, S H]]
-	[[Category: Shelley, C M.]]	+	[[Category: Shelley, C M]]
	[[Category: Beta beta alpha]]		[[Category: Beta beta alpha]]
	[[Category: Rna binding protein]]		[[Category: Rna binding protein]]
	[[Category: Rreiib-tr]]		[[Category: Rreiib-tr]]
	[[Category: Zinc finger protein]]		[[Category: Zinc finger protein]]

OCA at 07:08, 9 June 2014

OCA — Mon, 09 Jun 2014 07:08:11 GMT

←Older revision		Revision as of 07:08, 9 June 2014
Line 1:		Line 1:
-	[[~~Image:~~2ab7.~~png~~\|~~left~~\|~~200px~~]]	+	==Solution structures and characterization of HIV RRE IIB RNA targeting zinc finger proteins==
		+	<StructureSection load='2ab7' size='340' side='right' caption='[[2ab7]], [[NMR_Ensembles_of_Models \| 1 NMR models]]' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[2ab7]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AB7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2AB7 FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2ab3\|2ab3]]</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ab7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ab7 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2ab7 RCSB], [http://www.ebi.ac.uk/pdbsum/2ab7 PDBsum]</span></td></tr>
		+	<table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The Rev responsive element (RRE), a part of unspliced human immunodeficiency virus (HIV) RNA, serves a crucial role in the production of infectious HIV virions. The viral protein Rev binds to RRE and facilitates transport of mRNA to the cytoplasm. Inhibition of the Rev-RRE interaction disrupts the viral life cycle. Using a phage display protocol, dual zinc finger proteins (ZNFs) were generated that bind specifically to RREIIB at the high affinity Rev binding site. These proteins were further shortened and simplified, and they still retained their RNA binding affinity. The solution structures of ZNF29 and a mutant, ZNF29G29R, have been determined by nuclear magnetic resonance (NMR) spectroscopy. Both proteins form C(2)H(2)-type zinc fingers with essentially identical structures. RNA protein interactions were evaluated quantitatively by isothermal titration calorimetry, which revealed dissociation constants (K(d)'s) in the nanomolar range. The interaction with the RNA is dependent upon the zinc finger structure; in the presence of EDTA, RNA binding is abolished. For both proteins, RNA binding is mediated by the alpha-helical portion of the zinc fingers and target the bulge region of RREIIB-TR. However, ZNF29G29R exhibits significantly stronger binding to the RNA target than ZNF29; this illustrates that the binding of the zinc finger scaffold is amenable to further improvements.

-	~~{{STRUCTURE_2ab7\| PDB=2ab7 \| SCENE= }}~~	+	Solution structures and characterization of human immunodeficiency virus Rev responsive element IIB RNA targeting zinc finger proteins.,Mishra SH, Shelley CM, Barrow DJ Jr, Darby MK, Germann MW Biopolymers. 2006 Nov;83(4):352-64. PMID:16826557<ref>PMID:16826557</ref>

-	~~===Solution structures and characterization~~ of ~~HIV RRE IIB RNA targeting zinc finger proteins===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_16826557}}~~	+	== References ==
-		+	<references/>
-	~~==About this Structure==~~	+	__TOC__
-	~~[[2ab7]] is a 1 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Escherichia_coli Escherichia coli]~~. ~~Full experimental information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=2AB7 OCA].~~	+	</StructureSection>
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:016826557<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
	[[Category: Darby, M K.]]		[[Category: Darby, M K.]]

OCA: Protected "2ab7" [edit=sysop:move=sysop]

OCA — Tue, 14 Aug 2012 16:26:25 GMT

Protected "2ab7" [edit=sysop:move=sysop]

←Older revision

Revision as of 16:26, 14 August 2012

OCA at 16:26, 14 August 2012

OCA — Tue, 14 Aug 2012 16:26:23 GMT

←Older revision		Revision as of 16:26, 14 August 2012
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-	{{Seed}}
	[[Image:2ab7.png\|left\|200px]]		[[Image:2ab7.png\|left\|200px]]

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-	-->
	{{STRUCTURE_2ab7\| PDB=2ab7 \| SCENE= }}		{{STRUCTURE_2ab7\| PDB=2ab7 \| SCENE= }}

	===Solution structures and characterization of HIV RRE IIB RNA targeting zinc finger proteins===		===Solution structures and characterization of HIV RRE IIB RNA targeting zinc finger proteins===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_16826557}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 16826557 is the PubMed ID number.
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	{{ABSTRACT_PUBMED_16826557}}		{{ABSTRACT_PUBMED_16826557}}

	==About this Structure==		==About this Structure==
-	Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AB7 OCA].	+	[[2ab7]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AB7 OCA].

	==Reference==		==Reference==
-	Solution structures and characterization of human immunodeficiency virus Rev responsive element IIB RNA targeting zinc finger proteins., Mishra SH, Shelley CM, Barrow DJ Jr, Darby MK, Germann MW, Biopolymers. 2006 Nov;83(4):352-64. PMID:[~~http~~:~~//www.ncbi.nlm.nih.gov/pubmed/16826557 16826557~~]	+	<ref group="xtra">PMID:016826557</ref><references group="xtra"/>
		+	[[Category: Escherichia coli]]
	[[Category: Darby, M K.]]		[[Category: Darby, M K.]]
	[[Category: Germann, M W.]]		[[Category: Germann, M W.]]
Line 29:		Line 18:
	[[Category: Shelley, C M.]]		[[Category: Shelley, C M.]]
	[[Category: Beta beta alpha]]		[[Category: Beta beta alpha]]
		+	[[Category: Rna binding protein]]
	[[Category: Rreiib-tr]]		[[Category: Rreiib-tr]]
	[[Category: Zinc finger protein]]		[[Category: Zinc finger protein]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 17:08:41 2008''

OCA at 14:08, 28 July 2008

OCA — Mon, 28 Jul 2008 14:08:48 GMT

←Older revision		Revision as of 14:08, 28 July 2008
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-	[[Image:2ab7.~~gif~~\|left\|200px]]	+	{{Seed}}
		+	[[Image:2ab7.png\|left\|200px]]

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	{{STRUCTURE_2ab7\| PDB=2ab7 \| SCENE= }}		{{STRUCTURE_2ab7\| PDB=2ab7 \| SCENE= }}

-	~~'''~~Solution structures and characterization of HIV RRE IIB RNA targeting zinc finger proteins~~'''~~	+	===Solution structures and characterization of HIV RRE IIB RNA targeting zinc finger proteins===


-	~~==Overview==~~	+	<!--
-	The ~~Rev responsive element (RRE)~~, ~~a part of unspliced human immunodeficiency virus (HIV) RNA~~, ~~serves a crucial role in~~ the ~~production of infectious HIV virions. The viral protein Rev binds to RRE and facilitates transport of mRNA~~ to the ~~cytoplasm. Inhibition of the Rev-RRE interaction disrupts the viral life cycle. Using a phage display protocol, dual zinc finger proteins~~ (~~ZNFs) were generated that bind specifically to RREIIB~~ at ~~the high affinity Rev binding site~~. ~~These proteins were further shortened and simplified, and they still retained their RNA binding affinity~~. ~~The solution structures of ZNF29 and a mutant, ZNF29G29R, have been determined by nuclear magnetic resonance (NMR~~) ~~spectroscopy. Both proteins form C(2)H(2)-type zinc fingers with essentially identical structures. RNA protein interactions were evaluated quantitatively by isothermal titration calorimetry~~, ~~which revealed dissociation constants (K(d)'s) in the nanomolar range. The interaction with the RNA~~ is ~~dependent upon~~ the ~~zinc finger structure; in the presence of EDTA, RNA binding is abolished~~. ~~For both proteins, RNA binding is mediated by the alpha~~-~~helical portion of the zinc fingers and target the bulge region of RREIIB~~-~~TR. However, ZNF29G29R exhibits significantly stronger binding to the RNA target than ZNF29; this illustrates that the binding of the zinc finger scaffold is amenable to further improvements.~~	+	The line below this paragraph, {{ABSTRACT_PUBMED_16826557}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 16826557 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_16826557}}

	==About this Structure==		==About this Structure==
-	Full ~~crystallographic~~ information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AB7 OCA].	+	Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AB7 OCA].

	==Reference==		==Reference==
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	[[Category: Rreiib-tr]]		[[Category: Rreiib-tr]]
	[[Category: Zinc finger protein]]		[[Category: Zinc finger protein]]
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Sat May 3 18~~:50:37 2008''	+
		+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 17:08:41 2008''