2aef - Revision history

OCA at 07:23, 23 August 2023

OCA — Wed, 23 Aug 2023 07:23:03 GMT

←Older revision		Revision as of 07:23, 23 August 2023
Line 3:		Line 3:
	<StructureSection load='2aef' size='340' side='right'caption='[[2aef]], [[Resolution\|resolution]] 1.70Å' scene=''>		<StructureSection load='2aef' size='340' side='right'caption='[[2aef]], [[Resolution\|resolution]] 1.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2aef]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~"methanobacterium_thermoautotrophicus"_(sic)_zeikus_and_wolfe_1972 "methanobacterium thermoautotrophicus" (sic) zeikus and wolfe 1972~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AEF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2AEF FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2aef]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Methanothermobacter_thermautotrophicus Methanothermobacter thermautotrophicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AEF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2AEF FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.7Å</td></tr>
-	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"~~><div style~~=~~'overflow: auto; max-height: 3em;'~~>~~[[1lnq\|1lnq]], [[2aej\|2aej]], [[2aem\|2aem]]~~<~~/div></td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
-	~~<tr id~~='~~gene'><td class~~=~~"sblockLbl"><b>[[Gene\|Gene~~:~~]]</b~~></td>~~<td class="sblockDat">mthK ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=145262 "Methanobacterium thermoautotrophicus" (sic) Zeikus and Wolfe 1972])~~</td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2aef FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2aef OCA], [https://pdbe.org/2aef PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2aef RCSB], [https://www.ebi.ac.uk/pdbsum/2aef PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2aef ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2aef FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2aef OCA], [https://pdbe.org/2aef PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2aef RCSB], [https://www.ebi.ac.uk/pdbsum/2aef PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2aef ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/MTHK_METTH MTHK_METTH]] Calcium-gated potassium channel.	+	[https://www.uniprot.org/uniprot/MTHK_METTH MTHK_METTH] Calcium-gated potassium channel.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 38:		Line 37:
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Berke, I]]	+	[[Category: Methanothermobacter thermautotrophicus]]
-	[[Category: Chen, L]]	+	[[Category: Berke I]]
-	[[Category: Dong, J]]	+	[[Category: Chen L]]
-	[[Category: Jiang, Y]]	+	[[Category: Dong J]]
-	[[Category: Shi, N]]	+	[[Category: Jiang Y]]
-	~~[[Category: Bilobed architecture]]~~	+	[[Category: Shi N]]
-	~~[[Category: Ca2+ binding]]~~	+
-	~~[[Category: Flexible interface]]~~	+
-	~~[[Category: Helix-turn-helix]]~~	+
-	~~[[Category: Membrane protein]]~~	+
-	~~[[Category: Metal transport]]~~	+
-	~~[[Category: Rossmann fold~~]]	+

OCA at 08:30, 27 January 2021

OCA — Wed, 27 Jan 2021 08:30:14 GMT

←Older revision		Revision as of 08:30, 27 January 2021
Line 1:		Line 1:

	==Crystal Structures of the MthK RCK Domain in Ca2+ bound form==		==Crystal Structures of the MthK RCK Domain in Ca2+ bound form==
-	<StructureSection load='2aef' size='340' side='right' caption='[[2aef]], [[Resolution\|resolution]] 1.70Å' scene=''>	+	<StructureSection load='2aef' size='340' side='right'caption='[[2aef]], [[Resolution\|resolution]] 1.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2aef]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/"methanobacterium_thermoautotrophicus"_(sic)_zeikus_and_wolfe_1972 "methanobacterium thermoautotrophicus" (sic) zeikus and wolfe 1972]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AEF OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2AEF FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2aef]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/"methanobacterium_thermoautotrophicus"_(sic)_zeikus_and_wolfe_1972 "methanobacterium thermoautotrophicus" (sic) zeikus and wolfe 1972]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AEF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2AEF FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1lnq\|1lnq]], [[2aej\|2aej]], [[2aem\|2aem]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1lnq\|1lnq]], [[2aej\|2aej]], [[2aem\|2aem]]</div></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">mthK ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=145262 "Methanobacterium thermoautotrophicus" (sic) Zeikus and Wolfe 1972])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">mthK ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=145262 "Methanobacterium thermoautotrophicus" (sic) Zeikus and Wolfe 1972])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2aef FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2aef OCA], [~~http~~://pdbe.org/2aef PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2aef RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2aef PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2aef ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2aef FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2aef OCA], [https://pdbe.org/2aef PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2aef RCSB], [https://www.ebi.ac.uk/pdbsum/2aef PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2aef ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/MTHK_METTH MTHK_METTH]] Calcium-gated potassium channel.	+	[[https://www.uniprot.org/uniprot/MTHK_METTH MTHK_METTH]] Calcium-gated potassium channel.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 32:		Line 32:

	==See Also==		==See Also==
-	*[[Potassium ~~Channel~~\|Potassium ~~Channel~~]]	+	*[[Potassium channel 3D structures\|Potassium channel 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Berke, I]]		[[Category: Berke, I]]
	[[Category: Chen, L]]		[[Category: Chen, L]]

OCA at 06:58, 9 May 2018

OCA — Wed, 09 May 2018 06:58:36 GMT

←Older revision		Revision as of 06:58, 9 May 2018
Line 1:		Line 1:
		+
	==Crystal Structures of the MthK RCK Domain in Ca2+ bound form==		==Crystal Structures of the MthK RCK Domain in Ca2+ bound form==
	<StructureSection load='2aef' size='340' side='right' caption='[[2aef]], [[Resolution\|resolution]] 1.70Å' scene=''>		<StructureSection load='2aef' size='340' side='right' caption='[[2aef]], [[Resolution\|resolution]] 1.70Å' scene=''>
Line 6:		Line 7:
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1lnq\|1lnq]], [[2aej\|2aej]], [[2aem\|2aem]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1lnq\|1lnq]], [[2aej\|2aej]], [[2aem\|2aem]]</td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">mthK ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=145262 "Methanobacterium thermoautotrophicus" (sic) Zeikus and Wolfe 1972])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">mthK ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=145262 "Methanobacterium thermoautotrophicus" (sic) Zeikus and Wolfe 1972])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2aef FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2aef OCA], [http://pdbe.org/2aef PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2aef RCSB], [http://www.ebi.ac.uk/pdbsum/2aef PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2aef FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2aef OCA], [http://pdbe.org/2aef PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2aef RCSB], [http://www.ebi.ac.uk/pdbsum/2aef PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2aef ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 14:		Line 15:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ae/2aef_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ae/2aef_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2aef ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 15:13, 11 September 2015

OCA — Fri, 11 Sep 2015 15:13:04 GMT

←Older revision		Revision as of 15:13, 11 September 2015
Line 2:		Line 2:
	<StructureSection load='2aef' size='340' side='right' caption='[[2aef]], [[Resolution\|resolution]] 1.70Å' scene=''>		<StructureSection load='2aef' size='340' side='right' caption='[[2aef]], [[Resolution\|resolution]] 1.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2aef]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Methanothermobacter_thermautotrophicus Methanothermobacter thermautotrophicus~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AEF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2AEF FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2aef]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"methanobacterium_thermoautotrophicus"_(sic)_zeikus_and_wolfe_1972 "methanobacterium thermoautotrophicus" (sic) zeikus and wolfe 1972]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AEF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2AEF FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1lnq\|1lnq]], [[2aej\|2aej]], [[2aem\|2aem]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1lnq\|1lnq]], [[2aej\|2aej]], [[2aem\|2aem]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">mthK ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=145262 ~~Methanothermobacter thermautotrophicus~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">mthK ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=145262 "Methanobacterium thermoautotrophicus" (sic) Zeikus and Wolfe 1972])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2aef FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2aef OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2aef RCSB], [http://www.ebi.ac.uk/pdbsum/2aef PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2aef FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2aef OCA], [http://pdbe.org/2aef PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2aef RCSB], [http://www.ebi.ac.uk/pdbsum/2aef PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 28:		Line 28:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 2aef" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 35:		Line 36:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Methanothermobacter thermautotrophicus]]
	[[Category: Berke, I]]		[[Category: Berke, I]]
	[[Category: Chen, L]]		[[Category: Chen, L]]

OCA at 18:13, 25 December 2014

OCA — Thu, 25 Dec 2014 18:13:48 GMT

←Older revision		Revision as of 18:13, 25 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2aef]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Methanothermobacter_thermautotrophicus Methanothermobacter thermautotrophicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AEF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2AEF FirstGlance]. <br>		<table><tr><td colspan='2'>[[2aef]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Methanothermobacter_thermautotrophicus Methanothermobacter thermautotrophicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AEF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2AEF FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1lnq\|1lnq]], [[2aej\|2aej]], [[2aem\|2aem]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1lnq\|1lnq]], [[2aej\|2aej]], [[2aem\|2aem]]</td></tr>
-	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">mthK ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=145262 Methanothermobacter thermautotrophicus])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">mthK ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=145262 Methanothermobacter thermautotrophicus])</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2aef FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2aef OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2aef RCSB], [http://www.ebi.ac.uk/pdbsum/2aef PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2aef FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2aef OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2aef RCSB], [http://www.ebi.ac.uk/pdbsum/2aef PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/MTHK_METTH MTHK_METTH]] Calcium-gated potassium channel.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 34:		Line 36:
	</StructureSection>		</StructureSection>
	[[Category: Methanothermobacter thermautotrophicus]]		[[Category: Methanothermobacter thermautotrophicus]]
-	[[Category: Berke, I.]]	+	[[Category: Berke, I]]
-	[[Category: Chen, L.]]	+	[[Category: Chen, L]]
-	[[Category: Dong, J.]]	+	[[Category: Dong, J]]
-	[[Category: Jiang, Y.]]	+	[[Category: Jiang, Y]]
-	[[Category: Shi, N.]]	+	[[Category: Shi, N]]
	[[Category: Bilobed architecture]]		[[Category: Bilobed architecture]]
	[[Category: Ca2+ binding]]		[[Category: Ca2+ binding]]

OCA at 05:39, 29 September 2014

OCA — Mon, 29 Sep 2014 05:39:43 GMT

←Older revision		Revision as of 05:39, 29 September 2014
Line 1:		Line 1:
-	[[~~Image:~~2aef.~~png~~\|~~left~~\|200px]]	+	==Crystal Structures of the MthK RCK Domain in Ca2+ bound form==
		+	<StructureSection load='2aef' size='340' side='right' caption='[[2aef]], [[Resolution\|resolution]] 1.70Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[2aef]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Methanothermobacter_thermautotrophicus Methanothermobacter thermautotrophicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AEF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2AEF FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1lnq\|1lnq]], [[2aej\|2aej]], [[2aem\|2aem]]</td></tr>
		+	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">mthK ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=145262 Methanothermobacter thermautotrophicus])</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2aef FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2aef OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2aef RCSB], [http://www.ebi.ac.uk/pdbsum/2aef PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ae/2aef_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	MthK is a Ca2+-gated K+ channel from Methanobacterium autotrophicum. The crystal structure of the MthK channel in a Ca2+-bound open state was previously determined at 3.3 A and revealed an octameric gating ring composed of eight intracellular ligand-binding RCK (regulate the conductance of K+) domains. It was suggested that Ca2+ binding regulates the gating ring conformation, which in turn leads to the opening and closing of the channel. However, at 3.3 AA resolution, the molecular details of the structure are not well defined, and many of the conclusions drawn from that structure were hypothetical. Here we have presented high resolution structures of the MthK RCK domain with and without Ca2+ bound from three different crystals. These structures revealed a dimeric architecture of the RCK domain and allowed us to visualize the Ca2+ binding and protein-protein contacts at atomic detail. The dimerization of RCK domains is also conserved in other RCK-regulated K+ channels and transporters, suggesting that the RCK dimer serves as a basic unit in the gating ring assembly. A comparison of these dimer structures confirmed that the dimer interface is indeed flexible as suggested previously. However, the conformational change at the flexible interface is of an extent smaller than the previously hypothesized gating ring movement, and a reconstruction of these dimers into octamers by applying protein-protein contacts at the fixed interface did not generate enclosed gating rings. This indicated that there is a high probability that the previously defined fixed interface may not be fixed during channel gating. In addition to the structural studies, we have also carried out biochemical analyses and have shown that near physiological pH, isolated RCK domains form a stable octamer in solution, supporting the notion that the formation of octameric gating ring is a functionally relevant event in MthK gating. Additionally, our stability studies indicated that Ca2+ binding stabilizes the RCK domains in this octameric state.

-	~~{{STRUCTURE_2aef\| PDB=2aef \| SCENE= }}~~	+	Structures of the MthK RCK domain and the effect of Ca2+ on gating ring stability.,Dong J, Shi N, Berke I, Chen L, Jiang Y J Biol Chem. 2005 Dec 16;280(50):41716-24. Epub 2005 Oct 14. PMID:16227203<ref>PMID:16227203</ref>

-	~~===Crystal Structures~~ of the ~~MthK RCK Domain in Ca2+ bound form===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_16227203}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[2aef]] is a 2 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Methanothermobacter_thermautotrophicus Methanothermobacter thermautotrophicus]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=2AEF OCA].~~	+

	==See Also==		==See Also==
	*[[Potassium Channel\|Potassium Channel]]		*[[Potassium Channel\|Potassium Channel]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	<~~ref group="xtra">PMID:016227203<~~/~~ref~~><~~references group="xtra"~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Methanothermobacter thermautotrophicus]]		[[Category: Methanothermobacter thermautotrophicus]]
	[[Category: Berke, I.]]		[[Category: Berke, I.]]

OCA at 19:50, 20 October 2012

OCA — Sat, 20 Oct 2012 19:50:56 GMT

←Older revision		Revision as of 19:50, 20 October 2012
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	==About this Structure==		==About this Structure==
-	[[2aef]] is a 2 chain structure ~~of [[Potassium Channel]]~~ with sequence from [http://en.wikipedia.org/wiki/Methanothermobacter_thermautotrophicus Methanothermobacter thermautotrophicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AEF OCA].	+	[[2aef]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Methanothermobacter_thermautotrophicus Methanothermobacter thermautotrophicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AEF OCA].

	==See Also==		==See Also==

OCA at 18:55, 6 August 2012

OCA — Mon, 06 Aug 2012 18:55:32 GMT

←Older revision		Revision as of 18:55, 6 August 2012
Line 8:		Line 8:

	==About this Structure==		==About this Structure==
-	[[2aef]] is a 2 chain structure of ~~[[Ion channels (Part II)]] and~~ [[Potassium Channel]] with sequence from [http://en.wikipedia.org/wiki/Methanothermobacter_thermautotrophicus Methanothermobacter thermautotrophicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AEF OCA].	+	[[2aef]] is a 2 chain structure of [[Potassium Channel]] with sequence from [http://en.wikipedia.org/wiki/Methanothermobacter_thermautotrophicus Methanothermobacter thermautotrophicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AEF OCA].

	==See Also==		==See Also==
-	*[[Ion channels (Part II)\|Ion channels (Part II)]]
	*[[Potassium Channel\|Potassium Channel]]		*[[Potassium Channel\|Potassium Channel]]

OCA at 08:40, 27 July 2012

OCA — Fri, 27 Jul 2012 08:40:35 GMT

←Older revision		Revision as of 08:40, 27 July 2012
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	[[Image:2aef.png\|left\|200px]]		[[Image:2aef.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_2aef", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_2aef\| PDB=2aef \| SCENE= }}		{{STRUCTURE_2aef\| PDB=2aef \| SCENE= }}

	===Crystal Structures of the MthK RCK Domain in Ca2+ bound form===		===Crystal Structures of the MthK RCK Domain in Ca2+ bound form===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_16227203}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 16227203 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_16227203}}		{{ABSTRACT_PUBMED_16227203}}

	==About this Structure==		==About this Structure==
-	[[2aef]] is a 2 chain structure of [[Ion channels]] and [[~~Ion channels (Part II)~~]] with sequence from [http://en.wikipedia.org/wiki/Methanothermobacter_thermautotrophicus Methanothermobacter thermautotrophicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AEF OCA].	+	[[2aef]] is a 2 chain structure of [[Ion channels (Part II)]] and [[Potassium Channel]] with sequence from [http://en.wikipedia.org/wiki/Methanothermobacter_thermautotrophicus Methanothermobacter thermautotrophicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AEF OCA].

	==See Also==		==See Also==
-	*[[Ion channels]]	+	*[[Ion channels (Part II)\|Ion channels (Part II)]]
-	*[[Ion channels (Part II)]]	+	*[[Potassium Channel\|Potassium Channel]]

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~16227203~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:016227203</ref><references group="xtra"/>
	[[Category: Methanothermobacter thermautotrophicus]]		[[Category: Methanothermobacter thermautotrophicus]]
	[[Category: Berke, I.]]		[[Category: Berke, I.]]

OCA: Protected "2aef" [edit=sysop:move=sysop]

OCA — Mon, 27 Dec 2010 02:34:48 GMT

Protected "2aef" [edit=sysop:move=sysop]

←Older revision

Revision as of 02:34, 27 December 2010