2af8 - Revision history

OCA at 06:37, 1 May 2024

OCA — Wed, 01 May 2024 06:37:32 GMT

←Older revision		Revision as of 06:37, 1 May 2024
Line 1:		Line 1:

	==ACTINORHODIN POLYKETIDE SYNTHASE ACYL CARRIER PROTEIN FROM STREPTOMYCES COELICOLOR A3(2), NMR, MINIMIZED AVERAGE STRUCTURE==		==ACTINORHODIN POLYKETIDE SYNTHASE ACYL CARRIER PROTEIN FROM STREPTOMYCES COELICOLOR A3(2), NMR, MINIMIZED AVERAGE STRUCTURE==
-	<StructureSection load='2af8' size='340' side='right'caption='[[2af8~~]], [[NMR_Ensembles_of_Models \| 1 NMR models~~]]' scene=''>	+	<StructureSection load='2af8' size='340' side='right'caption='[[2af8]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2af8]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Strco Strco~~]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AF8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2AF8 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2af8]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_coelicolor_A3(2) Streptomyces coelicolor A3(2)]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AF8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2AF8 FirstGlance]. <br>
-	</td></tr><tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"~~><div style='overflow: auto; max-height: 3em;'>[[1af8\|1af8]]</div></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
-	~~<tr~~ id~~='gene'><td class~~="~~sblockLbl~~">~~<b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ACTINORHODIN ACYL CARRIER PROTEIN ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=100226 STRCO])~~</td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2af8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2af8 OCA], [https://pdbe.org/2af8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2af8 RCSB], [https://www.ebi.ac.uk/pdbsum/2af8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2af8 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2af8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2af8 OCA], [https://pdbe.org/2af8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2af8 RCSB], [https://www.ebi.ac.uk/pdbsum/2af8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2af8 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/ACPX_STRCO ACPX_STRCO]] Acyl carrier protein.	+	[https://www.uniprot.org/uniprot/ACPX_STRCO ACPX_STRCO] Acyl carrier protein.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 20:		Line 19:
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2af8 ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2af8 ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	The solution structure of the actinorhodin acyl carrier protein (act apo-ACP) from the polyketide synthase (PKS) of Streptomyces coelicolor A3(2) has been determined using 1H NMR spectroscopy, representing the first polyketide synthase component for which detailed structural information has been obtained. Twenty-four structures were generated by simulated annealing, employing 699 distance restraints and 94 dihedral angle restraints. The structure is composed, principally, of three major helices (1, 2, and 4), a shorter helix (3) and a large loop region separating helices 1 and 2. The structure is well-defined, except for a portion of the loop region (residues 18-29), the N-terminus (1-4), and a short stretch (57-61) in the loop connecting helices 2 and 3. The RMS distribution of the 24 structures about the average structure is 1.47 A for backbone atoms, 1.84 A for all heavy atoms (residues 5-86), and 1.01 A for backbone atoms over the helical regions (5-18, 41-86). The tertiary fold of act apo-ACP shows a strong structural homology with Escherichia coli fatty acid synthase (FAS) ACP, though some structural differences exist. First, there is no evidence that act apo-ACP is conformationally averaged between two or more states as observed in E. coli FAS ACP. Second, act apo-ACP shows a disordered N-terminus (residues 1-4) and a longer flexible loop (19-41 with 19-29 disordered) as opposed to E. coli FAS ACP where the N-terminal helix starts at residue 3 and the loop region is three amino acids shorter (16-35). Most importantly, however, although the act apo-ACP structure contains a hydrophobic core, there are in addition a number of buried hydrophilic groups, principally Arg72 and Asn79, both of which are 100% conserved in the PKS ACPs and not the FAS ACPs and may therefore play a role in stabilizing the growing polyketide chain. The structure-function relationship of act ACP is discussed in the light of these structural data and recent genetic advances in the field.
-
-	Solution structure of the actinorhodin polyketide synthase acyl carrier protein from Streptomyces coelicolor A3(2).,Crump MP, Crosby J, Dempsey CE, Parkinson JA, Murray M, Hopwood DA, Simpson TJ Biochemistry. 1997 May 20;36(20):6000-8. PMID:9166770<ref>PMID:9166770</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 2af8" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
	*[[Acyl carrier protein synthase 3D structures\|Acyl carrier protein synthase 3D structures]]		*[[Acyl carrier protein synthase 3D structures\|Acyl carrier protein synthase 3D structures]]
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	~~[[Category: Strco]]~~	+	[[Category: Crosby J]]
-	[[Category: Crosby, J]]	+	[[Category: Crump MP]]
-	[[Category: Crump~~, M P~~]]	+	[[Category: Dempsey CE]]
-	[[Category: Dempsey~~, C E~~]]	+	[[Category: Hopwood DA]]
-	[[Category: Hopwood~~, D A~~]]	+	[[Category: Murray M]]
-	[[Category: Murray, M]]	+	[[Category: Parkinson JA]]
-	[[Category: Parkinson~~, J A~~]]	+	[[Category: Simpson TJ]]
-	[[Category: Simpson~~, T J]]~~	+
-	~~[[Category: Acyl carrier protein]]~~	+
-	~~[[Category: Antibiotic biosynthesis]]~~	+
-	~~[[Category: Polyketide]]~~	+
-	~~[[Category: Solution structure]]~~	+
-	~~[[Category: Streptomyce~~]]	+

OCA at 08:30, 27 January 2021

OCA — Wed, 27 Jan 2021 08:30:48 GMT

←Older revision		Revision as of 08:30, 27 January 2021
Line 1:		Line 1:

	==ACTINORHODIN POLYKETIDE SYNTHASE ACYL CARRIER PROTEIN FROM STREPTOMYCES COELICOLOR A3(2), NMR, MINIMIZED AVERAGE STRUCTURE==		==ACTINORHODIN POLYKETIDE SYNTHASE ACYL CARRIER PROTEIN FROM STREPTOMYCES COELICOLOR A3(2), NMR, MINIMIZED AVERAGE STRUCTURE==
-	<StructureSection load='2af8' size='340' side='right' caption='[[2af8]], [[NMR_Ensembles_of_Models \| 1 NMR models]]' scene=''>	+	<StructureSection load='2af8' size='340' side='right'caption='[[2af8]], [[NMR_Ensembles_of_Models \| 1 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2af8]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Strco Strco]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AF8 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2AF8 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2af8]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Strco Strco]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AF8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2AF8 FirstGlance]. <br>
-	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1af8\|1af8]]</td></tr>	+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1af8\|1af8]]</div></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ACTINORHODIN ACYL CARRIER PROTEIN ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=100226 STRCO])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ACTINORHODIN ACYL CARRIER PROTEIN ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=100226 STRCO])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2af8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2af8 OCA], [~~http~~://pdbe.org/2af8 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2af8 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2af8 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2af8 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2af8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2af8 OCA], [https://pdbe.org/2af8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2af8 RCSB], [https://www.ebi.ac.uk/pdbsum/2af8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2af8 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/ACPX_STRCO ACPX_STRCO]] Acyl carrier protein.	+	[[https://www.uniprot.org/uniprot/ACPX_STRCO ACPX_STRCO]] Acyl carrier protein.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 31:		Line 31:

	==See Also==		==See Also==
-	*[[Acyl carrier protein synthase\|Acyl carrier protein synthase]]	+	*[[Acyl carrier protein synthase 3D structures\|Acyl carrier protein synthase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Strco]]		[[Category: Strco]]
	[[Category: Crosby, J]]		[[Category: Crosby, J]]

OCA at 06:53, 9 May 2018

OCA — Wed, 09 May 2018 06:53:52 GMT

←Older revision		Revision as of 06:53, 9 May 2018
Line 1:		Line 1:
		+
	==ACTINORHODIN POLYKETIDE SYNTHASE ACYL CARRIER PROTEIN FROM STREPTOMYCES COELICOLOR A3(2), NMR, MINIMIZED AVERAGE STRUCTURE==		==ACTINORHODIN POLYKETIDE SYNTHASE ACYL CARRIER PROTEIN FROM STREPTOMYCES COELICOLOR A3(2), NMR, MINIMIZED AVERAGE STRUCTURE==
	<StructureSection load='2af8' size='340' side='right' caption='[[2af8]], [[NMR_Ensembles_of_Models \| 1 NMR models]]' scene=''>		<StructureSection load='2af8' size='340' side='right' caption='[[2af8]], [[NMR_Ensembles_of_Models \| 1 NMR models]]' scene=''>
Line 5:		Line 6:
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1af8\|1af8]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1af8\|1af8]]</td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ACTINORHODIN ACYL CARRIER PROTEIN ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=100226 STRCO])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ACTINORHODIN ACYL CARRIER PROTEIN ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=100226 STRCO])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2af8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2af8 OCA], [http://pdbe.org/2af8 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2af8 RCSB], [http://www.ebi.ac.uk/pdbsum/2af8 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2af8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2af8 OCA], [http://pdbe.org/2af8 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2af8 RCSB], [http://www.ebi.ac.uk/pdbsum/2af8 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2af8 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 13:		Line 14:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/af/2af8_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/af/2af8_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2af8 ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 15:07, 11 September 2015

OCA — Fri, 11 Sep 2015 15:07:11 GMT

←Older revision		Revision as of 15:07, 11 September 2015
Line 2:		Line 2:
	<StructureSection load='2af8' size='340' side='right' caption='[[2af8]], [[NMR_Ensembles_of_Models \| 1 NMR models]]' scene=''>		<StructureSection load='2af8' size='340' side='right' caption='[[2af8]], [[NMR_Ensembles_of_Models \| 1 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2af8]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Streptomyces_coelicolor Streptomyces coelicolor~~]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AF8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2AF8 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2af8]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Strco Strco]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AF8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2AF8 FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1af8\|1af8]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1af8\|1af8]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ACTINORHODIN ACYL CARRIER PROTEIN ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=~~1902 Streptomyces coelicolor~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ACTINORHODIN ACYL CARRIER PROTEIN ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=100226 STRCO])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2af8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2af8 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2af8 RCSB], [http://www.ebi.ac.uk/pdbsum/2af8 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2af8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2af8 OCA], [http://pdbe.org/2af8 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2af8 RCSB], [http://www.ebi.ac.uk/pdbsum/2af8 PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 27:		Line 27:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 2af8" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 34:		Line 35:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Streptomyces coelicolor~~]]	+	[[Category: Strco]]
	[[Category: Crosby, J]]		[[Category: Crosby, J]]
	[[Category: Crump, M P]]		[[Category: Crump, M P]]

OCA at 18:10, 25 December 2014

OCA — Thu, 25 Dec 2014 18:10:01 GMT

←Older revision		Revision as of 18:10, 25 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2af8]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptomyces_coelicolor Streptomyces coelicolor]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AF8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2AF8 FirstGlance]. <br>		<table><tr><td colspan='2'>[[2af8]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptomyces_coelicolor Streptomyces coelicolor]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AF8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2AF8 FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1af8\|1af8]]</td></tr>	+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1af8\|1af8]]</td></tr>
-	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ACTINORHODIN ACYL CARRIER PROTEIN ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1902 Streptomyces coelicolor])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ACTINORHODIN ACYL CARRIER PROTEIN ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1902 Streptomyces coelicolor])</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2af8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2af8 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2af8 RCSB], [http://www.ebi.ac.uk/pdbsum/2af8 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2af8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2af8 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2af8 RCSB], [http://www.ebi.ac.uk/pdbsum/2af8 PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/ACPX_STRCO ACPX_STRCO]] Acyl carrier protein.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 33:		Line 35:
	</StructureSection>		</StructureSection>
	[[Category: Streptomyces coelicolor]]		[[Category: Streptomyces coelicolor]]
-	[[Category: Crosby, J.]]	+	[[Category: Crosby, J]]
-	[[Category: Crump, M P.]]	+	[[Category: Crump, M P]]
-	[[Category: Dempsey, C E.]]	+	[[Category: Dempsey, C E]]
-	[[Category: Hopwood, D A.]]	+	[[Category: Hopwood, D A]]
-	[[Category: Murray, M.]]	+	[[Category: Murray, M]]
-	[[Category: Parkinson, J A.]]	+	[[Category: Parkinson, J A]]
-	[[Category: Simpson, T J.]]	+	[[Category: Simpson, T J]]
	[[Category: Acyl carrier protein]]		[[Category: Acyl carrier protein]]
	[[Category: Antibiotic biosynthesis]]		[[Category: Antibiotic biosynthesis]]

OCA at 05:35, 29 September 2014

OCA — Mon, 29 Sep 2014 05:35:33 GMT

←Older revision		Revision as of 05:35, 29 September 2014
Line 1:		Line 1:
-	[[~~Image:~~2af8.~~png~~\|~~left~~\|200px]]	+	==ACTINORHODIN POLYKETIDE SYNTHASE ACYL CARRIER PROTEIN FROM STREPTOMYCES COELICOLOR A3(2), NMR, MINIMIZED AVERAGE STRUCTURE==
		+	<StructureSection load='2af8' size='340' side='right' caption='[[2af8]], [[NMR_Ensembles_of_Models \| 1 NMR models]]' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[2af8]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptomyces_coelicolor Streptomyces coelicolor]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AF8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2AF8 FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1af8\|1af8]]</td></tr>
		+	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ACTINORHODIN ACYL CARRIER PROTEIN ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1902 Streptomyces coelicolor])</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2af8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2af8 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2af8 RCSB], [http://www.ebi.ac.uk/pdbsum/2af8 PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/af/2af8_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The solution structure of the actinorhodin acyl carrier protein (act apo-ACP) from the polyketide synthase (PKS) of Streptomyces coelicolor A3(2) has been determined using 1H NMR spectroscopy, representing the first polyketide synthase component for which detailed structural information has been obtained. Twenty-four structures were generated by simulated annealing, employing 699 distance restraints and 94 dihedral angle restraints. The structure is composed, principally, of three major helices (1, 2, and 4), a shorter helix (3) and a large loop region separating helices 1 and 2. The structure is well-defined, except for a portion of the loop region (residues 18-29), the N-terminus (1-4), and a short stretch (57-61) in the loop connecting helices 2 and 3. The RMS distribution of the 24 structures about the average structure is 1.47 A for backbone atoms, 1.84 A for all heavy atoms (residues 5-86), and 1.01 A for backbone atoms over the helical regions (5-18, 41-86). The tertiary fold of act apo-ACP shows a strong structural homology with Escherichia coli fatty acid synthase (FAS) ACP, though some structural differences exist. First, there is no evidence that act apo-ACP is conformationally averaged between two or more states as observed in E. coli FAS ACP. Second, act apo-ACP shows a disordered N-terminus (residues 1-4) and a longer flexible loop (19-41 with 19-29 disordered) as opposed to E. coli FAS ACP where the N-terminal helix starts at residue 3 and the loop region is three amino acids shorter (16-35). Most importantly, however, although the act apo-ACP structure contains a hydrophobic core, there are in addition a number of buried hydrophilic groups, principally Arg72 and Asn79, both of which are 100% conserved in the PKS ACPs and not the FAS ACPs and may therefore play a role in stabilizing the growing polyketide chain. The structure-function relationship of act ACP is discussed in the light of these structural data and recent genetic advances in the field.

-	~~{{STRUCTURE_2af8\| PDB=2af8 \| SCENE= }}~~	+	Solution structure of the actinorhodin polyketide synthase acyl carrier protein from Streptomyces coelicolor A3(2).,Crump MP, Crosby J, Dempsey CE, Parkinson JA, Murray M, Hopwood DA, Simpson TJ Biochemistry. 1997 May 20;36(20):6000-8. PMID:9166770<ref>PMID:9166770</ref>

-	~~===ACTINORHODIN POLYKETIDE SYNTHASE ACYL CARRIER PROTEIN FROM STREPTOMYCES COELICOLOR A3(2)~~, ~~NMR, MINIMIZED AVERAGE STRUCTURE===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_9166770}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[2af8]] is~~ a ~~1 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Streptomyces_coelicolor Streptomyces coelicolor]~~. ~~Full experimental information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=2AF8 OCA].~~	+

	==See Also==		==See Also==
	*[[Acyl carrier protein synthase\|Acyl carrier protein synthase]]		*[[Acyl carrier protein synthase\|Acyl carrier protein synthase]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	<~~ref group="xtra">PMID:009166770<~~/~~ref~~><~~ref group="xtra">PMID:017168537</ref><references group="xtra"~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Streptomyces coelicolor]]		[[Category: Streptomyces coelicolor]]
	[[Category: Crosby, J.]]		[[Category: Crosby, J.]]

OCA at 19:48, 20 October 2012

OCA — Sat, 20 Oct 2012 19:48:45 GMT

←Older revision		Revision as of 19:48, 20 October 2012
Line 8:		Line 8:

	==About this Structure==		==About this Structure==
-	[[2af8]] is a 1 chain structure ~~of [[Acyl carrier protein synthase]]~~ with sequence from [http://en.wikipedia.org/wiki/Streptomyces_coelicolor Streptomyces coelicolor]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AF8 OCA].	+	[[2af8]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptomyces_coelicolor Streptomyces coelicolor]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AF8 OCA].

	==See Also==		==See Also==

OCA: Protected "2af8" [edit=sysop:move=sysop]

OCA — Fri, 27 Jul 2012 08:34:00 GMT

Protected "2af8" [edit=sysop:move=sysop]

←Older revision

Revision as of 08:34, 27 July 2012

OCA at 08:33, 27 July 2012

OCA — Fri, 27 Jul 2012 08:33:58 GMT

←Older revision		Revision as of 08:33, 27 July 2012
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-	{{Seed}}
	[[Image:2af8.png\|left\|200px]]		[[Image:2af8.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_2af8", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
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	{{STRUCTURE_2af8\| PDB=2af8 \| SCENE= }}		{{STRUCTURE_2af8\| PDB=2af8 \| SCENE= }}

	===ACTINORHODIN POLYKETIDE SYNTHASE ACYL CARRIER PROTEIN FROM STREPTOMYCES COELICOLOR A3(2), NMR, MINIMIZED AVERAGE STRUCTURE===		===ACTINORHODIN POLYKETIDE SYNTHASE ACYL CARRIER PROTEIN FROM STREPTOMYCES COELICOLOR A3(2), NMR, MINIMIZED AVERAGE STRUCTURE===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_9166770}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 9166770 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_9166770}}		{{ABSTRACT_PUBMED_9166770}}

	==About this Structure==		==About this Structure==
-	~~2AF8~~ is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Streptomyces_coelicolor Streptomyces coelicolor]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AF8 OCA].	+	[[2af8]] is a 1 chain structure of [[Acyl carrier protein synthase]] with sequence from [http://en.wikipedia.org/wiki/Streptomyces_coelicolor Streptomyces coelicolor]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AF8 OCA].
		+
		+	==See Also==
		+	*[[Acyl carrier protein synthase\|Acyl carrier protein synthase]]

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~9166770~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:009166770</ref><ref group="xtra">PMID:017168537</ref><references group="xtra"/>
	[[Category: Streptomyces coelicolor]]		[[Category: Streptomyces coelicolor]]
	[[Category: Crosby, J.]]		[[Category: Crosby, J.]]
Line 34:		Line 25:
	[[Category: Acyl carrier protein]]		[[Category: Acyl carrier protein]]
	[[Category: Antibiotic biosynthesis]]		[[Category: Antibiotic biosynthesis]]
-	[[Category: Nmr structure]]
	[[Category: Polyketide]]		[[Category: Polyketide]]
	[[Category: Solution structure]]		[[Category: Solution structure]]
	[[Category: Streptomyce]]		[[Category: Streptomyce]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Feb 18 01:20:40 2009''

OCA at 23:20, 17 February 2009

OCA — Tue, 17 Feb 2009 23:20:45 GMT

←Older revision		Revision as of 23:20, 17 February 2009
Line 20:		Line 20:

	==About this Structure==		==About this Structure==
-	2AF8 is a ~~[[Single protein]]~~ structure of sequence from [http://en.wikipedia.org/wiki/Streptomyces_coelicolor Streptomyces coelicolor]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AF8 OCA].	+	2AF8 is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Streptomyces_coelicolor Streptomyces coelicolor]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AF8 OCA].

	==Reference==		==Reference==
-	Solution structure of the actinorhodin polyketide synthase acyl carrier protein from Streptomyces coelicolor A3(2)., Crump MP, Crosby J, Dempsey CE, Parkinson JA, Murray M, Hopwood DA, Simpson TJ, Biochemistry. 1997 May 20;36(20):6000-8. PMID:~~[http:~~//~~www.ncbi.nlm.nih.gov/pubmed/9166770 9166770]~~	+	<ref group="xtra">PMID:9166770</ref><references group="xtra"/>
-	~~[[Category: Single protein]]~~	+
	[[Category: Streptomyces coelicolor]]		[[Category: Streptomyces coelicolor]]
	[[Category: Crosby, J.]]		[[Category: Crosby, J.]]
Line 40:		Line 39:
	[[Category: Streptomyce]]		[[Category: Streptomyce]]

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Tue Jul 29 08~~:35:~~27 2008~~''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Feb 18 01:20:40 2009''