2ahw - Revision history

OCA at 07:24, 23 August 2023

OCA — Wed, 23 Aug 2023 07:24:32 GMT

←Older revision		Revision as of 07:24, 23 August 2023
Line 3:		Line 3:
	<StructureSection load='2ahw' size='340' side='right'caption='[[2ahw]], [[Resolution\|resolution]] 2.15Å' scene=''>		<StructureSection load='2ahw' size='340' side='right'caption='[[2ahw]], [[Resolution\|resolution]] 2.15Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2ahw]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Eco57 Eco57~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AHW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2AHW FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2ahw]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_O157:H7 Escherichia coli O157:H7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AHW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2AHW FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=COA:COENZYME+A'>COA</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.15Å</td></tr>
-	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"~~><div style~~=~~'overflow: auto; max-height: 3em;'~~>~~[[2ahu\|2ahu]], [[2ahv\|2ahv]]~~<~~/div></td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=COA:COENZYME+A'>COA</scene></td></tr>
-	~~<tr id~~='~~gene'><td class~~=~~"sblockLbl"><b>[[Gene\|Gene~~:~~]]</b~~></td>~~<td class="sblockDat">YdiF ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83334 ECO57])~~</td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ahw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ahw OCA], [https://pdbe.org/2ahw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ahw RCSB], [https://www.ebi.ac.uk/pdbsum/2ahw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ahw ProSAT], [https://www.topsan.org/Proteins/BSGI/2ahw TOPSAN]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ahw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ahw OCA], [https://pdbe.org/2ahw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ahw RCSB], [https://www.ebi.ac.uk/pdbsum/2ahw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ahw ProSAT], [https://www.topsan.org/Proteins/BSGI/2ahw TOPSAN]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/YDIF_ECO57 YDIF_ECO57]] CoA transferase having broad substrate specificity for short-chain acyl-CoA thioesters with the activity decreasing when the length of the carboxylic acid chain exceeds four carbons. Exhibits high activity with acetoacetyl-CoA, propionyl-CoA, crotonoyl-CoA or butyryl-CoA as donors, with acetate as an acceptor. When acetyl-CoA is used as the donor, propionate, acetoacetate, butyrate, isobutyrate, and 4-hydroxybutyrate can be utilized as acceptors but not isovalerate. May play a role in short-chain fatty acid metabolism in E.coli.<ref>PMID:16253988</ref>	+	[https://www.uniprot.org/uniprot/YDIF_ECO57 YDIF_ECO57] CoA transferase having broad substrate specificity for short-chain acyl-CoA thioesters with the activity decreasing when the length of the carboxylic acid chain exceeds four carbons. Exhibits high activity with acetoacetyl-CoA, propionyl-CoA, crotonoyl-CoA or butyryl-CoA as donors, with acetate as an acceptor. When acetyl-CoA is used as the donor, propionate, acetoacetate, butyrate, isobutyrate, and 4-hydroxybutyrate can be utilized as acceptors but not isovalerate. May play a role in short-chain fatty acid metabolism in E.coli.<ref>PMID:16253988</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 34:		Line 33:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Eco57~~]]	+	[[Category: Escherichia coli O157:H7]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Ajamian, E]]	+	[[Category: Ajamian E]]
-	~~[[Category: Structural genomic~~]]	+	[[Category: Cylger M]]
-	[[Category: Cylger, M]]	+	[[Category: Fraser ME]]
-	[[Category: Fraser~~, M E~~]]	+	[[Category: Iannuzzi P]]
-	[[Category: Iannuzzi, P]]	+	[[Category: Kernaghan SD]]
-	[[Category: Kernaghan~~, S D~~]]	+	[[Category: Li Y]]
-	[[Category: Li, Y]]	+	[[Category: Matte A]]
-	[[Category: Matte, A]]	+	[[Category: Rangarajan ES]]
-	[[Category: Rangarajan~~, E S]]~~	+
-	~~[[Category: Bsgi]]~~	+
-	~~[[Category: Coa transferase]]~~	+
-	~~[[Category: Glutamyl thioester]]~~	+
-	~~[[Category: Transferase]]~~	+
-	~~[[Category: Ydif~~]]	+

OCA at 08:32, 27 January 2021

OCA — Wed, 27 Jan 2021 08:32:04 GMT

←Older revision		Revision as of 08:32, 27 January 2021
Line 1:		Line 1:

	==Crystal Structure of Acyl-CoA transferase from E. coli O157:H7 (YdiF)-thioester complex with CoA- 2==		==Crystal Structure of Acyl-CoA transferase from E. coli O157:H7 (YdiF)-thioester complex with CoA- 2==
-	<StructureSection load='2ahw' size='340' side='right' caption='[[2ahw]], [[Resolution\|resolution]] 2.15Å' scene=''>	+	<StructureSection load='2ahw' size='340' side='right'caption='[[2ahw]], [[Resolution\|resolution]] 2.15Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2ahw]] is a 4 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Eco57 Eco57]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AHW OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2AHW FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2ahw]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Eco57 Eco57]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AHW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2AHW FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=COA:COENZYME+A'>COA</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=COA:COENZYME+A'>COA</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2ahu\|2ahu]], [[2ahv\|2ahv]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2ahu\|2ahu]], [[2ahv\|2ahv]]</div></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">YdiF ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83334 ECO57])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">YdiF ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83334 ECO57])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2ahw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ahw OCA], [~~http~~://pdbe.org/2ahw PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2ahw RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2ahw PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2ahw ProSAT], [~~http~~://www.topsan.org/Proteins/BSGI/2ahw TOPSAN]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ahw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ahw OCA], [https://pdbe.org/2ahw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ahw RCSB], [https://www.ebi.ac.uk/pdbsum/2ahw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ahw ProSAT], [https://www.topsan.org/Proteins/BSGI/2ahw TOPSAN]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/YDIF_ECO57 YDIF_ECO57]] CoA transferase having broad substrate specificity for short-chain acyl-CoA thioesters with the activity decreasing when the length of the carboxylic acid chain exceeds four carbons. Exhibits high activity with acetoacetyl-CoA, propionyl-CoA, crotonoyl-CoA or butyryl-CoA as donors, with acetate as an acceptor. When acetyl-CoA is used as the donor, propionate, acetoacetate, butyrate, isobutyrate, and 4-hydroxybutyrate can be utilized as acceptors but not isovalerate. May play a role in short-chain fatty acid metabolism in E.coli.<ref>PMID:16253988</ref>	+	[[https://www.uniprot.org/uniprot/YDIF_ECO57 YDIF_ECO57]] CoA transferase having broad substrate specificity for short-chain acyl-CoA thioesters with the activity decreasing when the length of the carboxylic acid chain exceeds four carbons. Exhibits high activity with acetoacetyl-CoA, propionyl-CoA, crotonoyl-CoA or butyryl-CoA as donors, with acetate as an acceptor. When acetyl-CoA is used as the donor, propionate, acetoacetate, butyrate, isobutyrate, and 4-hydroxybutyrate can be utilized as acceptors but not isovalerate. May play a role in short-chain fatty acid metabolism in E.coli.<ref>PMID:16253988</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 35:		Line 35:
	</StructureSection>		</StructureSection>
	[[Category: Eco57]]		[[Category: Eco57]]
		+	[[Category: Large Structures]]
	[[Category: Ajamian, E]]		[[Category: Ajamian, E]]
	[[Category: Structural genomic]]		[[Category: Structural genomic]]

OCA at 07:23, 9 May 2018

OCA — Wed, 09 May 2018 07:23:33 GMT

←Older revision		Revision as of 07:23, 9 May 2018
Line 1:		Line 1:
		+
	==Crystal Structure of Acyl-CoA transferase from E. coli O157:H7 (YdiF)-thioester complex with CoA- 2==		==Crystal Structure of Acyl-CoA transferase from E. coli O157:H7 (YdiF)-thioester complex with CoA- 2==
	<StructureSection load='2ahw' size='340' side='right' caption='[[2ahw]], [[Resolution\|resolution]] 2.15Å' scene=''>		<StructureSection load='2ahw' size='340' side='right' caption='[[2ahw]], [[Resolution\|resolution]] 2.15Å' scene=''>
Line 6:		Line 7:
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2ahu\|2ahu]], [[2ahv\|2ahv]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2ahu\|2ahu]], [[2ahv\|2ahv]]</td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">YdiF ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83334 ECO57])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">YdiF ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83334 ECO57])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ahw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ahw OCA], [http://pdbe.org/2ahw PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2ahw RCSB], [http://www.ebi.ac.uk/pdbsum/2ahw PDBsum], [http://www.topsan.org/Proteins/BSGI/2ahw TOPSAN]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ahw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ahw OCA], [http://pdbe.org/2ahw PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2ahw RCSB], [http://www.ebi.ac.uk/pdbsum/2ahw PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2ahw ProSAT], [http://www.topsan.org/Proteins/BSGI/2ahw TOPSAN]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 14:		Line 15:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ah/2ahw_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ah/2ahw_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2ahw ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 08:04, 11 September 2015

OCA — Fri, 11 Sep 2015 08:04:21 GMT

←Older revision		Revision as of 08:04, 11 September 2015
Line 2:		Line 2:
	<StructureSection load='2ahw' size='340' side='right' caption='[[2ahw]], [[Resolution\|resolution]] 2.15Å' scene=''>		<StructureSection load='2ahw' size='340' side='right' caption='[[2ahw]], [[Resolution\|resolution]] 2.15Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2ahw]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Escherichia_coli Escherichia coli~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AHW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2AHW FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2ahw]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Eco57 Eco57]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AHW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2AHW FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=COA:COENZYME+A'>COA</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=COA:COENZYME+A'>COA</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2ahu\|2ahu]], [[2ahv\|2ahv]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2ahu\|2ahu]], [[2ahv\|2ahv]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">YdiF ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=~~562 Escherichia coli~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">YdiF ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83334 ECO57])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ahw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ahw OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2ahw RCSB], [http://www.ebi.ac.uk/pdbsum/2ahw PDBsum], [http://www.topsan.org/Proteins/BSGI/2ahw TOPSAN]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ahw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ahw OCA], [http://pdbe.org/2ahw PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2ahw RCSB], [http://www.ebi.ac.uk/pdbsum/2ahw PDBsum], [http://www.topsan.org/Proteins/BSGI/2ahw TOPSAN]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[http://www.uniprot.org/uniprot/~~Q8X5X6_ECO57 Q8X5X6_ECO57~~]] CoA transferase having broad substrate specificity for short-chain acyl-CoA thioesters with the activity decreasing when the length of the carboxylic acid chain exceeds four carbons. Exhibits high activity with acetoacetyl-CoA, propionyl-CoA, crotonoyl-CoA or butyryl-CoA as donors, with acetate as an acceptor. When acetyl-CoA is used as the donor, propionate, acetoacetate, butyrate, isobutyrate, and 4-hydroxybutyrate can be utilized as acceptors but not isovalerate. May play a role in short-chain fatty acid metabolism in E.coli.	+	[[http://www.uniprot.org/uniprot/YDIF_ECO57 YDIF_ECO57]] CoA transferase having broad substrate specificity for short-chain acyl-CoA thioesters with the activity decreasing when the length of the carboxylic acid chain exceeds four carbons. Exhibits high activity with acetoacetyl-CoA, propionyl-CoA, crotonoyl-CoA or butyryl-CoA as donors, with acetate as an acceptor. When acetyl-CoA is used as the donor, propionate, acetoacetate, butyrate, isobutyrate, and 4-hydroxybutyrate can be utilized as acceptors but not isovalerate. May play a role in short-chain fatty acid metabolism in E.coli.<ref>PMID:16253988</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 28:		Line 28:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 2ahw" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Escherichia coli~~]]	+	[[Category: Eco57]]
	[[Category: Ajamian, E]]		[[Category: Ajamian, E]]
	[[Category: Structural genomic]]		[[Category: Structural genomic]]

OCA at 13:37, 25 December 2014

OCA — Thu, 25 Dec 2014 13:37:09 GMT

←Older revision		Revision as of 13:37, 25 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2ahw]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AHW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2AHW FirstGlance]. <br>		<table><tr><td colspan='2'>[[2ahw]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AHW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2AHW FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=COA:COENZYME+A'>COA</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=COA:COENZYME+A'>COA</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2ahu\|2ahu]], [[2ahv\|2ahv]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2ahu\|2ahu]], [[2ahv\|2ahv]]</td></tr>
-	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">YdiF ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">YdiF ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ahw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ahw OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2ahw RCSB], [http://www.ebi.ac.uk/pdbsum/2ahw PDBsum], [http://www.topsan.org/Proteins/BSGI/2ahw TOPSAN]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ahw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ahw OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2ahw RCSB], [http://www.ebi.ac.uk/pdbsum/2ahw PDBsum], [http://www.topsan.org/Proteins/BSGI/2ahw TOPSAN]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/Q8X5X6_ECO57 Q8X5X6_ECO57]] CoA transferase having broad substrate specificity for short-chain acyl-CoA thioesters with the activity decreasing when the length of the carboxylic acid chain exceeds four carbons. Exhibits high activity with acetoacetyl-CoA, propionyl-CoA, crotonoyl-CoA or butyryl-CoA as donors, with acetate as an acceptor. When acetyl-CoA is used as the donor, propionate, acetoacetate, butyrate, isobutyrate, and 4-hydroxybutyrate can be utilized as acceptors but not isovalerate. May play a role in short-chain fatty acid metabolism in E.coli.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 31:		Line 33:
	</StructureSection>		</StructureSection>
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
-	[[Category: Ajamian, E.]]	+	[[Category: Ajamian, E]]
-	[[Category: ~~BSGI, Montreal-Kingston Bacterial~~ Structural ~~Genomics Initiative.~~]]	+	[[Category: Structural genomic]]
-	[[Category: Cylger, M.]]	+	[[Category: Cylger, M]]
-	[[Category: Fraser, M E.]]	+	[[Category: Fraser, M E]]
-	[[Category: Iannuzzi, P.]]	+	[[Category: Iannuzzi, P]]
-	[[Category: Kernaghan, S D.]]	+	[[Category: Kernaghan, S D]]
-	[[Category: Li, Y.]]	+	[[Category: Li, Y]]
-	[[Category: Matte, A.]]	+	[[Category: Matte, A]]
-	[[Category: Rangarajan, E S.]]	+	[[Category: Rangarajan, E S]]
	[[Category: Bsgi]]		[[Category: Bsgi]]
	[[Category: Coa transferase]]		[[Category: Coa transferase]]
	[[Category: Glutamyl thioester]]		[[Category: Glutamyl thioester]]
-	[[Category: Montreal-kingston bacterial structural genomics initiative]]
-	[[Category: Structural genomic]]
	[[Category: Transferase]]		[[Category: Transferase]]
	[[Category: Ydif]]		[[Category: Ydif]]

OCA at 00:31, 30 September 2014

OCA — Tue, 30 Sep 2014 00:31:04 GMT

←Older revision		Revision as of 00:31, 30 September 2014
Line 1:		Line 1:
-	[[~~Image:~~2ahw.~~png~~\|~~left~~\|200px]]	+	==Crystal Structure of Acyl-CoA transferase from E. coli O157:H7 (YdiF)-thioester complex with CoA- 2==
		+	<StructureSection load='2ahw' size='340' side='right' caption='[[2ahw]], [[Resolution\|resolution]] 2.15Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[2ahw]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AHW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2AHW FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=COA:COENZYME+A'>COA</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2ahu\|2ahu]], [[2ahv\|2ahv]]</td></tr>
		+	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">YdiF ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ahw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ahw OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2ahw RCSB], [http://www.ebi.ac.uk/pdbsum/2ahw PDBsum], [http://www.topsan.org/Proteins/BSGI/2ahw TOPSAN]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ah/2ahw_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Coenzyme A transferases are involved in a broad range of biochemical processes in both prokaryotes and eukaryotes, and exhibit a diverse range of substrate specificities. The YdiF protein from Escherichia coli O157:H7 is an acyl-CoA transferase of unknown physiological function, and belongs to a large sequence family of CoA transferases, present in bacteria to humans, which utilize oxoacids as acceptors. In vitro measurements showed that YdiF displays enzymatic activity with short-chain acyl-CoAs. The crystal structures of YdiF and its complex with CoA, the first co-crystal structure for any Family I CoA transferase, have been determined and refined at 1.9 and 2.0 A resolution, respectively. YdiF is organized into tetramers, with each monomer having an open alpha/beta structure characteristic of Family I CoA transferases. Co-crystallization of YdiF with a variety of CoA thioesters in the absence of acceptor carboxylic acid resulted in trapping a covalent gamma-glutamyl-CoA thioester intermediate. The CoA binds within a well defined pocket at the N- and C-terminal domain interface, but makes contact only with the C-terminal domain. The structure of the YdiF complex provides a basis for understanding the different catalytic steps in the reaction of Family I CoA transferases.

-	~~{{STRUCTURE_2ahw\| PDB=2ahw \| SCENE= }}~~	+	Crystallographic trapping of the glutamyl-CoA thioester intermediate of family I CoA transferases.,Rangarajan ES, Li Y, Ajamian E, Iannuzzi P, Kernaghan SD, Fraser ME, Cygler M, Matte A J Biol Chem. 2005 Dec 30;280(52):42919-28. Epub 2005 Oct 27. PMID:16253988<ref>PMID:16253988</ref>

-	~~===Crystal Structure~~ of ~~Acyl-CoA transferase from E~~. ~~coli O157:H7 (YdiF)-thioester complex with CoA- 2===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_16253988}}~~	+	== References ==
-		+	<references/>
-	~~==About this Structure==~~	+	__TOC__
-	~~[[2ahw]] is a 4 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org~~/~~wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AHW OCA].~~	+	</StructureSection>
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:016253988<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
	[[Category: Ajamian, E.]]		[[Category: Ajamian, E.]]

OCA: Protected "2ahw" [edit=sysop:move=sysop]

OCA — Sun, 06 Jan 2013 09:53:24 GMT

Protected "2ahw" [edit=sysop:move=sysop]

←Older revision

Revision as of 09:53, 6 January 2013

OCA at 09:53, 6 January 2013

OCA — Sun, 06 Jan 2013 09:53:21 GMT

←Older revision		Revision as of 09:53, 6 January 2013
Line 1:		Line 1:
-	{{Seed}}
	[[Image:2ahw.png\|left\|200px]]		[[Image:2ahw.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_2ahw", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_2ahw\| PDB=2ahw \| SCENE= }}		{{STRUCTURE_2ahw\| PDB=2ahw \| SCENE= }}

	===Crystal Structure of Acyl-CoA transferase from E. coli O157:H7 (YdiF)-thioester complex with CoA- 2===		===Crystal Structure of Acyl-CoA transferase from E. coli O157:H7 (YdiF)-thioester complex with CoA- 2===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_16253988}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 16253988 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_16253988}}		{{ABSTRACT_PUBMED_16253988}}

	==About this Structure==		==About this Structure==
-	~~2AHW~~ is a 4 ~~chains~~ structure ~~of sequences~~ from [http://en.wikipedia.org/wiki/~~Escherichia_coli_o157:h7~~ Escherichia coli ~~o157:h7~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AHW OCA].	+	[[2ahw]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AHW OCA].

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~16253988~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:016253988</ref><references group="xtra"/>
-	[[Category: Escherichia coli ~~o157:h7~~]]	+	[[Category: Escherichia coli]]
	[[Category: Ajamian, E.]]		[[Category: Ajamian, E.]]
	[[Category: BSGI, Montreal-Kingston Bacterial Structural Genomics Initiative.]]		[[Category: BSGI, Montreal-Kingston Bacterial Structural Genomics Initiative.]]
Line 39:		Line 27:
	[[Category: Montreal-kingston bacterial structural genomics initiative]]		[[Category: Montreal-kingston bacterial structural genomics initiative]]
	[[Category: Structural genomic]]		[[Category: Structural genomic]]
		+	[[Category: Transferase]]
	[[Category: Ydif]]		[[Category: Ydif]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 17:35:47 2009''

OCA at 15:35, 17 February 2009

OCA — Tue, 17 Feb 2009 15:35:48 GMT

←Older revision		Revision as of 15:35, 17 February 2009
Line 23:		Line 23:

	==Reference==		==Reference==
-	Crystallographic trapping of the glutamyl-CoA thioester intermediate of family I CoA transferases., Rangarajan ES, Li Y, Ajamian E, Iannuzzi P, Kernaghan SD, Fraser ME, Cygler M, Matte A, J Biol Chem. 2005 Dec 30;280(52):42919-28. Epub 2005 Oct 27. PMID:~~[http:~~//~~www.ncbi.nlm.nih.gov/pubmed/16253988 16253988]~~	+	<ref group="xtra">PMID:16253988</ref><references group="xtra"/>
	[[Category: Escherichia coli o157:h7]]		[[Category: Escherichia coli o157:h7]]
	[[Category: Ajamian, E.]]		[[Category: Ajamian, E.]]
Line 41:		Line 41:
	[[Category: Ydif]]		[[Category: Ydif]]

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Wed Dec 10 15~~:45:~~53 2008~~''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 17:35:47 2009''

OCA at 13:45, 10 December 2008

OCA — Wed, 10 Dec 2008 13:45:53 GMT

←Older revision		Revision as of 13:45, 10 December 2008
Line 25:		Line 25:
	Crystallographic trapping of the glutamyl-CoA thioester intermediate of family I CoA transferases., Rangarajan ES, Li Y, Ajamian E, Iannuzzi P, Kernaghan SD, Fraser ME, Cygler M, Matte A, J Biol Chem. 2005 Dec 30;280(52):42919-28. Epub 2005 Oct 27. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16253988 16253988]		Crystallographic trapping of the glutamyl-CoA thioester intermediate of family I CoA transferases., Rangarajan ES, Li Y, Ajamian E, Iannuzzi P, Kernaghan SD, Fraser ME, Cygler M, Matte A, J Biol Chem. 2005 Dec 30;280(52):42919-28. Epub 2005 Oct 27. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16253988 16253988]
	[[Category: Escherichia coli o157:h7]]		[[Category: Escherichia coli o157:h7]]
-	[[Category: ~~Pdbx_ordinal=~~, ~~<PDBx~~:~~audit_author~~.]]	+	[[Category: Ajamian, E.]]
		+	[[Category: BSGI, Montreal-Kingston Bacterial Structural Genomics Initiative.]]
		+	[[Category: Cylger, M.]]
		+	[[Category: Fraser, M E.]]
		+	[[Category: Iannuzzi, P.]]
		+	[[Category: Kernaghan, S D.]]
		+	[[Category: Li, Y.]]
		+	[[Category: Matte, A.]]
		+	[[Category: Rangarajan, E S.]]
	[[Category: Bsgi]]		[[Category: Bsgi]]
	[[Category: Coa transferase]]		[[Category: Coa transferase]]
Line 33:		Line 41:
	[[Category: Ydif]]		[[Category: Ydif]]

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Fri~~ Dec ~~5 09~~:44:22 2008''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Dec 10 15:45:53 2008''