2ajj - Revision history

OCA at 09:14, 9 May 2024

OCA — Thu, 09 May 2024 09:14:58 GMT

←Older revision		Revision as of 09:14, 9 May 2024
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2ajj]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bovine_viral_diarrhea_virus_1-CP7 Bovine viral diarrhea virus 1-CP7]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AJJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2AJJ FirstGlance]. <br>		<table><tr><td colspan='2'>[[2ajj]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bovine_viral_diarrhea_virus_1-CP7 Bovine viral diarrhea virus 1-CP7]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AJJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2AJJ FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ajj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ajj OCA], [https://pdbe.org/2ajj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ajj RCSB], [https://www.ebi.ac.uk/pdbsum/2ajj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ajj ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ajj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ajj OCA], [https://pdbe.org/2ajj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ajj RCSB], [https://www.ebi.ac.uk/pdbsum/2ajj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ajj ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==

OCA at 08:24, 14 June 2023

OCA — Wed, 14 Jun 2023 08:24:48 GMT

←Older revision		Revision as of 08:24, 14 June 2023
Line 1:		Line 1:

	==NMR structure of the in-plane membrane anchor domain [1-28] of the monotopic Non Structural Protein 5A (NS5A) of Bovine Viral Diarrhea Virus (BVDV)==		==NMR structure of the in-plane membrane anchor domain [1-28] of the monotopic Non Structural Protein 5A (NS5A) of Bovine Viral Diarrhea Virus (BVDV)==
-	<StructureSection load='2ajj' size='340' side='right'caption='[[2ajj~~]], [[NMR_Ensembles_of_Models \| 31 NMR models~~]]' scene=''>	+	<StructureSection load='2ajj' size='340' side='right'caption='[[2ajj]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2ajj]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AJJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2AJJ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2ajj]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bovine_viral_diarrhea_virus_1-CP7 Bovine viral diarrhea virus 1-CP7]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AJJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2AJJ FirstGlance]. <br>
-	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2ajn\|2ajn]], [[2ajm\|2ajm]], [[2ajo\|2ajo]]</div></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ajj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ajj OCA], [https://pdbe.org/2ajj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ajj RCSB], [https://www.ebi.ac.uk/pdbsum/2ajj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ajj ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ajj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ajj OCA], [https://pdbe.org/2ajj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ajj RCSB], [https://www.ebi.ac.uk/pdbsum/2ajj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ajj ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/POLG_BVDVC POLG_BVDVC]] Initial binding to target cell probably involves interaction of E(rns) with glycosaminoglycans. E1 and/or E2 are responsible of cell attachment with CD46 and subsequent fusion after internalization of the virion by endocytosis (By similarity). P7 forms a leader sequence to properly orient NS2 in the membrane (By similarity). Uncleaved NS2-3 is required for production of infectious virus. NS2 protease seems to play a vital role in viral RNA replication control and in the pathogenicity of the virus. NS3 displays three enzymatic activities: serine protease, NTPase and RNA helicase. NS4A is a cofactor for the NS3 protease activity (By similarity). RNA-directed RNA polymerase NS5 replicates the viral (+) and (-) genome.	+	[https://www.uniprot.org/uniprot/POLG_BVDVC POLG_BVDVC] Initial binding to target cell probably involves interaction of E(rns) with glycosaminoglycans. E1 and/or E2 are responsible of cell attachment with CD46 and subsequent fusion after internalization of the virion by endocytosis (By similarity). P7 forms a leader sequence to properly orient NS2 in the membrane (By similarity). Uncleaved NS2-3 is required for production of infectious virus. NS2 protease seems to play a vital role in viral RNA replication control and in the pathogenicity of the virus. NS3 displays three enzymatic activities: serine protease, NTPase and RNA helicase. NS4A is a cofactor for the NS3 protease activity (By similarity). RNA-directed RNA polymerase NS5 replicates the viral (+) and (-) genome.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 25:		Line 24:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Bovine viral diarrhea virus 1-CP7]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Brass, V]]	+	[[Category: Brass V]]
-	[[Category: Chipot, C]]	+	[[Category: Chipot C]]
-	[[Category: Deleage, G]]	+	[[Category: Deleage G]]
-	[[Category: Montserret, R]]	+	[[Category: Montserret R]]
-	[[Category: Moradpour, D]]	+	[[Category: Moradpour D]]
-	[[Category: Penin, F]]	+	[[Category: Penin F]]
-	[[Category: Sapay, N]]	+	[[Category: Sapay N]]
-	~~[[Category: Amphipathic alpha-helix]]~~	+
-	~~[[Category: In-plane membrane anchor domain]]~~	+
-	~~[[Category: Membrane protein~~]]	+

OCA at 08:27, 10 November 2021

OCA — Wed, 10 Nov 2021 08:27:21 GMT

←Older revision		Revision as of 08:27, 10 November 2021
Line 3:		Line 3:
	<StructureSection load='2ajj' size='340' side='right'caption='[[2ajj]], [[NMR_Ensembles_of_Models \| 31 NMR models]]' scene=''>		<StructureSection load='2ajj' size='340' side='right'caption='[[2ajj]], [[NMR_Ensembles_of_Models \| 31 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2ajj]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AJJ OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2AJJ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2ajj]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AJJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2AJJ FirstGlance]. <br>
-	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2ajn\|2ajn]], [[2ajm\|2ajm]], [[2ajo\|2ajo]]</td></tr>	+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2ajn\|2ajn]], [[2ajm\|2ajm]], [[2ajo\|2ajo]]</div></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2ajj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ajj OCA], [~~http~~://pdbe.org/2ajj PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2ajj RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2ajj PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2ajj ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ajj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ajj OCA], [https://pdbe.org/2ajj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ajj RCSB], [https://www.ebi.ac.uk/pdbsum/2ajj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ajj ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/POLG_BVDVC POLG_BVDVC]] Initial binding to target cell probably involves interaction of E(rns) with glycosaminoglycans. E1 and/or E2 are responsible of cell attachment with CD46 and subsequent fusion after internalization of the virion by endocytosis (By similarity). P7 forms a leader sequence to properly orient NS2 in the membrane (By similarity). Uncleaved NS2-3 is required for production of infectious virus. NS2 protease seems to play a vital role in viral RNA replication control and in the pathogenicity of the virus. NS3 displays three enzymatic activities: serine protease, NTPase and RNA helicase. NS4A is a cofactor for the NS3 protease activity (By similarity). RNA-directed RNA polymerase NS5 replicates the viral (+) and (-) genome.	+	[[https://www.uniprot.org/uniprot/POLG_BVDVC POLG_BVDVC]] Initial binding to target cell probably involves interaction of E(rns) with glycosaminoglycans. E1 and/or E2 are responsible of cell attachment with CD46 and subsequent fusion after internalization of the virion by endocytosis (By similarity). P7 forms a leader sequence to properly orient NS2 in the membrane (By similarity). Uncleaved NS2-3 is required for production of infectious virus. NS2 protease seems to play a vital role in viral RNA replication control and in the pathogenicity of the virus. NS3 displays three enzymatic activities: serine protease, NTPase and RNA helicase. NS4A is a cofactor for the NS3 protease activity (By similarity). RNA-directed RNA polymerase NS5 replicates the viral (+) and (-) genome.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==

OCA at 08:22, 5 February 2020

OCA — Wed, 05 Feb 2020 08:22:13 GMT

←Older revision		Revision as of 08:22, 5 February 2020
Line 1:		Line 1:

	==NMR structure of the in-plane membrane anchor domain [1-28] of the monotopic Non Structural Protein 5A (NS5A) of Bovine Viral Diarrhea Virus (BVDV)==		==NMR structure of the in-plane membrane anchor domain [1-28] of the monotopic Non Structural Protein 5A (NS5A) of Bovine Viral Diarrhea Virus (BVDV)==
-	<StructureSection load='2ajj' size='340' side='right' caption='[[2ajj]], [[NMR_Ensembles_of_Models \| 31 NMR models]]' scene=''>	+	<StructureSection load='2ajj' size='340' side='right'caption='[[2ajj]], [[NMR_Ensembles_of_Models \| 31 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2ajj]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AJJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2AJJ FirstGlance]. <br>		<table><tr><td colspan='2'>[[2ajj]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AJJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2AJJ FirstGlance]. <br>
Line 20:		Line 20:

	==See Also==		==See Also==
-	*[[Nonstructural protein\|Nonstructural protein]]	+	*[[Nonstructural protein 3D structures\|Nonstructural protein 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Brass, V]]		[[Category: Brass, V]]
	[[Category: Chipot, C]]		[[Category: Chipot, C]]

OCA at 07:12, 9 May 2018

OCA — Wed, 09 May 2018 07:12:27 GMT

←Older revision		Revision as of 07:12, 9 May 2018
Line 1:		Line 1:
		+
	==NMR structure of the in-plane membrane anchor domain [1-28] of the monotopic Non Structural Protein 5A (NS5A) of Bovine Viral Diarrhea Virus (BVDV)==		==NMR structure of the in-plane membrane anchor domain [1-28] of the monotopic Non Structural Protein 5A (NS5A) of Bovine Viral Diarrhea Virus (BVDV)==
	<StructureSection load='2ajj' size='340' side='right' caption='[[2ajj]], [[NMR_Ensembles_of_Models \| 31 NMR models]]' scene=''>		<StructureSection load='2ajj' size='340' side='right' caption='[[2ajj]], [[NMR_Ensembles_of_Models \| 31 NMR models]]' scene=''>
Line 4:		Line 5:
	<table><tr><td colspan='2'>[[2ajj]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AJJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2AJJ FirstGlance]. <br>		<table><tr><td colspan='2'>[[2ajj]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AJJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2AJJ FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2ajn\|2ajn]], [[2ajm\|2ajm]], [[2ajo\|2ajo]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2ajn\|2ajn]], [[2ajm\|2ajm]], [[2ajo\|2ajo]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ajj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ajj OCA], [http://pdbe.org/2ajj PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2ajj RCSB], [http://www.ebi.ac.uk/pdbsum/2ajj PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ajj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ajj OCA], [http://pdbe.org/2ajj PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2ajj RCSB], [http://www.ebi.ac.uk/pdbsum/2ajj PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2ajj ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==

OCA at 04:10, 11 September 2015

OCA — Fri, 11 Sep 2015 04:10:42 GMT

←Older revision		Revision as of 04:10, 11 September 2015
Line 4:		Line 4:
	<table><tr><td colspan='2'>[[2ajj]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AJJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2AJJ FirstGlance]. <br>		<table><tr><td colspan='2'>[[2ajj]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AJJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2AJJ FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2ajn\|2ajn]], [[2ajm\|2ajm]], [[2ajo\|2ajo]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2ajn\|2ajn]], [[2ajm\|2ajm]], [[2ajo\|2ajo]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ajj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ajj OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2ajj RCSB], [http://www.ebi.ac.uk/pdbsum/2ajj PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ajj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ajj OCA], [http://pdbe.org/2ajj PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2ajj RCSB], [http://www.ebi.ac.uk/pdbsum/2ajj PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 16:		Line 16:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 2ajj" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==

OCA at 10:57, 25 December 2014

OCA — Thu, 25 Dec 2014 10:57:48 GMT

←Older revision		Revision as of 10:57, 25 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2ajj]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AJJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2AJJ FirstGlance]. <br>		<table><tr><td colspan='2'>[[2ajj]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AJJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2AJJ FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2ajn\|2ajn]], [[2ajm\|2ajm]], [[2ajo\|2ajo]]</td></tr>	+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2ajn\|2ajn]], [[2ajm\|2ajm]], [[2ajo\|2ajo]]</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ajj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ajj OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2ajj RCSB], [http://www.ebi.ac.uk/pdbsum/2ajj PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ajj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ajj OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2ajj RCSB], [http://www.ebi.ac.uk/pdbsum/2ajj PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/POLG_BVDVC POLG_BVDVC]] Initial binding to target cell probably involves interaction of E(rns) with glycosaminoglycans. E1 and/or E2 are responsible of cell attachment with CD46 and subsequent fusion after internalization of the virion by endocytosis (By similarity). P7 forms a leader sequence to properly orient NS2 in the membrane (By similarity). Uncleaved NS2-3 is required for production of infectious virus. NS2 protease seems to play a vital role in viral RNA replication control and in the pathogenicity of the virus. NS3 displays three enzymatic activities: serine protease, NTPase and RNA helicase. NS4A is a cofactor for the NS3 protease activity (By similarity). RNA-directed RNA polymerase NS5 replicates the viral (+) and (-) genome.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 21:		Line 23:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Brass, V.]]	+	[[Category: Brass, V]]
-	[[Category: Chipot, C.]]	+	[[Category: Chipot, C]]
-	[[Category: Deleage, G.]]	+	[[Category: Deleage, G]]
-	[[Category: Montserret, R.]]	+	[[Category: Montserret, R]]
-	[[Category: Moradpour, D.]]	+	[[Category: Moradpour, D]]
-	[[Category: Penin, F.]]	+	[[Category: Penin, F]]
-	[[Category: Sapay, N.]]	+	[[Category: Sapay, N]]
	[[Category: Amphipathic alpha-helix]]		[[Category: Amphipathic alpha-helix]]
	[[Category: In-plane membrane anchor domain]]		[[Category: In-plane membrane anchor domain]]
	[[Category: Membrane protein]]		[[Category: Membrane protein]]

OCA at 00:57, 2 October 2014

OCA — Thu, 02 Oct 2014 00:57:07 GMT

←Older revision		Revision as of 00:57, 2 October 2014
Line 1:		Line 1:
-	[[~~Image:~~2ajj.~~png~~\|~~left~~\|~~200px~~]]	+	==NMR structure of the in-plane membrane anchor domain [1-28] of the monotopic Non Structural Protein 5A (NS5A) of Bovine Viral Diarrhea Virus (BVDV)==
		+	<StructureSection load='2ajj' size='340' side='right' caption='[[2ajj]], [[NMR_Ensembles_of_Models \| 31 NMR models]]' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[2ajj]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AJJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2AJJ FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2ajn\|2ajn]], [[2ajm\|2ajm]], [[2ajo\|2ajo]]</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ajj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ajj OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2ajj RCSB], [http://www.ebi.ac.uk/pdbsum/2ajj PDBsum]</span></td></tr>
		+	<table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Hepatitis C virus (HCV) nonstructural protein 5A (NS5A) is a monotopic membrane protein anchored to the membrane by an N-terminal in-plane amphipathic alpha-helix. This membrane anchor is essential for the assembly of a functional viral replication complex. Although amino acid sequences differ considerably, putative membrane anchors with amphipathic features were predicted in NS5A from related Flaviviridae family members, in particular bovine viral diarrhea virus (BVDV), the prototype representative of the genus Pestivirus. We report here the NMR structure of the membrane anchor 1-28 of NS5A from BVDV in the presence of different membrane mimetic media. This anchor includes a long amphipathic alpha-helix of 21 residues interacting in-plane with the membrane interface and including a putative flexible region. Molecular dynamic simulation at a water-dodecane interface used to mimic the surface separating a lipid bilayer and an aqueous medium demonstrated the stability of the helix orientation and the location at the hydrophobic-hydrophilic interface. The flexible region of the helix appears to be required to allow the most favorable interaction of hydrophobic and hydrophilic side chain residues with their respective environment at the membrane interface. Despite the lack of amino acid sequence similarity, this amphipathic helix shares common structural features with that of the HCV counterpart, including a stable, hydrophobic N-terminal segment separated from the more hydrophilic C-terminal segment by a local, flexible region. These structural conservations point toward conserved roles of the N-terminal in-plane membrane anchors of NS5A in replication complex formation of HCV, BVDV, and other related viruses.

-	~~{{STRUCTURE_2ajj\| PDB=2ajj \| SCENE= }}~~	+	NMR structure and molecular dynamics of the in-plane membrane anchor of nonstructural protein 5A from bovine viral diarrhea virus.,Sapay N, Montserret R, Chipot C, Brass V, Moradpour D, Deleage G, Penin F Biochemistry. 2006 Feb 21;45(7):2221-33. PMID:16475810<ref>PMID:16475810</ref>

-	~~===NMR structure~~ of the ~~in-plane membrane anchor domain [1-28] of the monotopic Non Structural Protein 5A (NS5A) of Bovine Viral Diarrhea Virus (BVDV)===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_16475810}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[2ajj]] is a 1 chain structure~~. ~~Full experimental information is available from [http://oca~~.~~weizmann~~.~~ac.il~~/~~oca-bin/ocashort?id=2AJJ OCA].~~	+

	==See Also==		==See Also==
	*[[Nonstructural protein\|Nonstructural protein]]		*[[Nonstructural protein\|Nonstructural protein]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	<~~ref group="xtra">PMID:016475810<~~/~~ref~~><~~references group="xtra"~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Brass, V.]]		[[Category: Brass, V.]]
	[[Category: Chipot, C.]]		[[Category: Chipot, C.]]

OCA at 15:53, 20 October 2012

OCA — Sat, 20 Oct 2012 15:53:47 GMT

←Older revision		Revision as of 15:53, 20 October 2012
Line 8:		Line 8:

	==About this Structure==		==About this Structure==
-	[[2ajj]] is a 1 chain structure ~~of [[Nonstructural protein]]~~. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AJJ OCA].	+	[[2ajj]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AJJ OCA].

	==See Also==		==See Also==

OCA: Protected "2ajj" [edit=sysop:move=sysop]

OCA — Thu, 26 Jul 2012 19:50:08 GMT

Protected "2ajj" [edit=sysop:move=sysop]

←Older revision

Revision as of 19:50, 26 July 2012