2b2c - Revision history

OCA at 13:42, 13 March 2024

OCA — Wed, 13 Mar 2024 13:42:34 GMT

←Older revision		Revision as of 13:42, 13 March 2024
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	<StructureSection load='2b2c' size='340' side='right'caption='[[2b2c]], [[Resolution\|resolution]] 2.50Å' scene=''>		<StructureSection load='2b2c' size='340' side='right'caption='[[2b2c]], [[Resolution\|resolution]] 2.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2b2c]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Caeel Caeel~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2B2C OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2B2C FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2b2c]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Caenorhabditis_elegans Caenorhabditis elegans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2B2C OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2B2C FirstGlance]. <br>
-	</td></tr><tr id='~~activity~~'><td class="sblockLbl"><b>~~Activity~~:</b></td><td class="sblockDat"~~><span class~~=~~'plainlinks'~~>[~~https://en.wikipedia.org/wiki/Spermidine_synthase Spermidine synthase~~]~~, with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=~~2.5~~.1.16 2.5.1.16] </span>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.5Å</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2b2c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2b2c OCA], [https://pdbe.org/2b2c PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2b2c RCSB], [https://www.ebi.ac.uk/pdbsum/2b2c PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2b2c ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2b2c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2b2c OCA], [https://pdbe.org/2b2c PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2b2c RCSB], [https://www.ebi.ac.uk/pdbsum/2b2c PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2b2c ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/G5EFG3_CAEEL G5EFG3_CAEEL]
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2b2c ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2b2c ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	The polyamine synthesis enzyme spermidine synthase (SPDS) has been cloned from the model nematode Caenorhabditis elegans. Biochemical characterisation of the recombinantly expressed protein revealed a high degree of similarity to other eukaryotic SPDS with the exception of a low affinity towards the substrate decarboxylated S-adenosylmethionine (Km = 110 microM) and a less pronounced feedback inhibition by the second reaction product 5'-methylthioadenosine (IC50 = 430 microM). The C. elegans protein that carries a nematode-specific insertion of 27 amino acids close to its N-terminus was crystallized, leading to the first X-ray structure of a dimeric eukaryotic SPDS.
-
-	Cloning, expression, characterisation and three-dimensional structure determination of Caenorhabditis elegans spermidine synthase.,Dufe VT, Luersen K, Eschbach ML, Haider N, Karlberg T, Walter RD, Al-Karadaghi S FEBS Lett. 2005 Nov 7;579(27):6037-43. Epub 2005 Oct 5. PMID:16226262<ref>PMID:16226262</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 2b2c" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
	*[[Spermidine synthase 3D structures\|Spermidine synthase 3D structures]]		*[[Spermidine synthase 3D structures\|Spermidine synthase 3D structures]]
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Caeel~~]]	+	[[Category: Caenorhabditis elegans]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	~~[[Category: Spermidine synthase]]~~	+	[[Category: Al-Karadaghi S]]
-	[[Category: Al-Karadaghi, S]]	+	[[Category: Dufe VT]]
-	[[Category: Dufe~~, V T~~]]	+	[[Category: Eschbach ML]]
-	[[Category: Eschbach~~, M L~~]]	+	[[Category: Haider N]]
-	[[Category: Haider, N]]	+	[[Category: Karlberg T]]
-	[[Category: Karlberg, T]]	+	[[Category: Luersen K]]
-	[[Category: Luersen, K]]	+	[[Category: Walter RD]]
-	[[Category: Walter~~, R D]]~~	+
-	~~[[Category: Beta-alpha]]~~	+
-	~~[[Category: Transferase~~]]	+

OCA at 09:42, 5 May 2021

OCA — Wed, 05 May 2021 09:42:21 GMT

←Older revision		Revision as of 09:42, 5 May 2021
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	<StructureSection load='2b2c' size='340' side='right'caption='[[2b2c]], [[Resolution\|resolution]] 2.50Å' scene=''>		<StructureSection load='2b2c' size='340' side='right'caption='[[2b2c]], [[Resolution\|resolution]] 2.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2b2c]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Caeel Caeel]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2B2C OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2B2C FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2b2c]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Caeel Caeel]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2B2C OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2B2C FirstGlance]. <br>
-	</td></tr><tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Spermidine_synthase Spermidine synthase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.16 2.5.1.16] </span></td></tr>	+	</td></tr><tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Spermidine_synthase Spermidine synthase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.16 2.5.1.16] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2b2c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2b2c OCA], [~~http~~://pdbe.org/2b2c PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2b2c RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2b2c PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2b2c ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2b2c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2b2c OCA], [https://pdbe.org/2b2c PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2b2c RCSB], [https://www.ebi.ac.uk/pdbsum/2b2c PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2b2c ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
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	==See Also==		==See Also==
-	*[[Spermidine ~~Synthase~~\|Spermidine ~~Synthase~~]]	+	*[[Spermidine synthase 3D structures\|Spermidine synthase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 09:34, 24 July 2019

OCA — Wed, 24 Jul 2019 09:34:07 GMT

←Older revision		Revision as of 09:34, 24 July 2019
Line 1:		Line 1:

	==Cloning, expression, characterisation and three- dimensional structure determination of the Caenorhabditis elegans spermidine synthase==		==Cloning, expression, characterisation and three- dimensional structure determination of the Caenorhabditis elegans spermidine synthase==
-	<StructureSection load='2b2c' size='340' side='right' caption='[[2b2c]], [[Resolution\|resolution]] 2.50Å' scene=''>	+	<StructureSection load='2b2c' size='340' side='right'caption='[[2b2c]], [[Resolution\|resolution]] 2.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2b2c]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Caeel Caeel]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2B2C OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2B2C FirstGlance]. <br>		<table><tr><td colspan='2'>[[2b2c]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Caeel Caeel]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2B2C OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2B2C FirstGlance]. <br>
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	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/b2/2b2c_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/b2/2b2c_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
Line 26:		Line 26:
	</div>		</div>
	<div class="pdbe-citations 2b2c" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 2b2c" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Spermidine Synthase\|Spermidine Synthase]]
	== References ==		== References ==
	<references/>		<references/>
Line 31:		Line 34:
	</StructureSection>		</StructureSection>
	[[Category: Caeel]]		[[Category: Caeel]]
		+	[[Category: Large Structures]]
	[[Category: Spermidine synthase]]		[[Category: Spermidine synthase]]
	[[Category: Al-Karadaghi, S]]		[[Category: Al-Karadaghi, S]]

OCA at 16:31, 12 October 2017

OCA — Thu, 12 Oct 2017 16:31:50 GMT

←Older revision		Revision as of 16:31, 12 October 2017
Line 1:		Line 1:
		+
	==Cloning, expression, characterisation and three- dimensional structure determination of the Caenorhabditis elegans spermidine synthase==		==Cloning, expression, characterisation and three- dimensional structure determination of the Caenorhabditis elegans spermidine synthase==
	<StructureSection load='2b2c' size='340' side='right' caption='[[2b2c]], [[Resolution\|resolution]] 2.50Å' scene=''>		<StructureSection load='2b2c' size='340' side='right' caption='[[2b2c]], [[Resolution\|resolution]] 2.50Å' scene=''>
Line 4:		Line 5:
	<table><tr><td colspan='2'>[[2b2c]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Caeel Caeel]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2B2C OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2B2C FirstGlance]. <br>		<table><tr><td colspan='2'>[[2b2c]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Caeel Caeel]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2B2C OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2B2C FirstGlance]. <br>
	</td></tr><tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Spermidine_synthase Spermidine synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.16 2.5.1.16] </span></td></tr>		</td></tr><tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Spermidine_synthase Spermidine synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.16 2.5.1.16] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2b2c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2b2c OCA], [http://pdbe.org/2b2c PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2b2c RCSB], [http://www.ebi.ac.uk/pdbsum/2b2c PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2b2c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2b2c OCA], [http://pdbe.org/2b2c PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2b2c RCSB], [http://www.ebi.ac.uk/pdbsum/2b2c PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2b2c ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
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	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2b2c ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 25:		Line 26:
	</div>		</div>
	<div class="pdbe-citations 2b2c" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 2b2c" style="background-color:#fffaf0;"></div>
-
-	==See Also==
-	*[[Spermidine Synthase\|Spermidine Synthase]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 10:16, 11 September 2015

OCA — Fri, 11 Sep 2015 10:16:24 GMT

←Older revision		Revision as of 10:16, 11 September 2015
Line 2:		Line 2:
	<StructureSection load='2b2c' size='340' side='right' caption='[[2b2c]], [[Resolution\|resolution]] 2.50Å' scene=''>		<StructureSection load='2b2c' size='340' side='right' caption='[[2b2c]], [[Resolution\|resolution]] 2.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2b2c]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Caenorhabditis_elegans Caenorhabditis elegans~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2B2C OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2B2C FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2b2c]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Caeel Caeel]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2B2C OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2B2C FirstGlance]. <br>
	</td></tr><tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Spermidine_synthase Spermidine synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.16 2.5.1.16] </span></td></tr>		</td></tr><tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Spermidine_synthase Spermidine synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.16 2.5.1.16] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2b2c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2b2c OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2b2c RCSB], [http://www.ebi.ac.uk/pdbsum/2b2c PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2b2c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2b2c OCA], [http://pdbe.org/2b2c PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2b2c RCSB], [http://www.ebi.ac.uk/pdbsum/2b2c PDBsum]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
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	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 2b2c" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 31:		Line 32:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Caenorhabditis elegans~~]]	+	[[Category: Caeel]]
	[[Category: Spermidine synthase]]		[[Category: Spermidine synthase]]
	[[Category: Al-Karadaghi, S]]		[[Category: Al-Karadaghi, S]]

OCA at 10:39, 8 January 2015

OCA — Thu, 08 Jan 2015 10:39:37 GMT

←Older revision		Revision as of 10:39, 8 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2b2c]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Caenorhabditis_elegans Caenorhabditis elegans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2B2C OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2B2C FirstGlance]. <br>		<table><tr><td colspan='2'>[[2b2c]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Caenorhabditis_elegans Caenorhabditis elegans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2B2C OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2B2C FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Spermidine_synthase Spermidine synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.16 2.5.1.16] </span></td></tr>	+	</td></tr><tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Spermidine_synthase Spermidine synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.16 2.5.1.16] </span></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2b2c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2b2c OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2b2c RCSB], [http://www.ebi.ac.uk/pdbsum/2b2c PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2b2c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2b2c OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2b2c RCSB], [http://www.ebi.ac.uk/pdbsum/2b2c PDBsum]</span></td></tr>
-	<table>	+	</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	[[Category: Caenorhabditis elegans]]		[[Category: Caenorhabditis elegans]]
	[[Category: Spermidine synthase]]		[[Category: Spermidine synthase]]
-	[[Category: Al-Karadaghi, S.]]	+	[[Category: Al-Karadaghi, S]]
-	[[Category: Dufe, V T.]]	+	[[Category: Dufe, V T]]
-	[[Category: Eschbach, M L.]]	+	[[Category: Eschbach, M L]]
-	[[Category: Haider, N.]]	+	[[Category: Haider, N]]
-	[[Category: Karlberg, T.]]	+	[[Category: Karlberg, T]]
-	[[Category: Luersen, K.]]	+	[[Category: Luersen, K]]
-	[[Category: Walter, R D.]]	+	[[Category: Walter, R D]]
	[[Category: Beta-alpha]]		[[Category: Beta-alpha]]
	[[Category: Transferase]]		[[Category: Transferase]]

OCA at 10:26, 29 September 2014

OCA — Mon, 29 Sep 2014 10:26:11 GMT

←Older revision		Revision as of 10:26, 29 September 2014
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-	[[~~Image~~:2b2c.~~png\|left~~\|200px]]	+	==Cloning, expression, characterisation and three- dimensional structure determination of the Caenorhabditis elegans spermidine synthase==
		+	<StructureSection load='2b2c' size='340' side='right' caption='[[2b2c]], [[Resolution\|resolution]] 2.50Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[2b2c]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Caenorhabditis_elegans Caenorhabditis elegans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2B2C OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2B2C FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Spermidine_synthase Spermidine synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.16 2.5.1.16] </span></td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2b2c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2b2c OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2b2c RCSB], [http://www.ebi.ac.uk/pdbsum/2b2c PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/b2/2b2c_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The polyamine synthesis enzyme spermidine synthase (SPDS) has been cloned from the model nematode Caenorhabditis elegans. Biochemical characterisation of the recombinantly expressed protein revealed a high degree of similarity to other eukaryotic SPDS with the exception of a low affinity towards the substrate decarboxylated S-adenosylmethionine (Km = 110 microM) and a less pronounced feedback inhibition by the second reaction product 5'-methylthioadenosine (IC50 = 430 microM). The C. elegans protein that carries a nematode-specific insertion of 27 amino acids close to its N-terminus was crystallized, leading to the first X-ray structure of a dimeric eukaryotic SPDS.

-	~~{{STRUCTURE_2b2c\| PDB=2b2c \| SCENE= }}~~	+	Cloning, expression, characterisation and three-dimensional structure determination of Caenorhabditis elegans spermidine synthase.,Dufe VT, Luersen K, Eschbach ML, Haider N, Karlberg T, Walter RD, Al-Karadaghi S FEBS Lett. 2005 Nov 7;579(27):6037-43. Epub 2005 Oct 5. PMID:16226262<ref>PMID:16226262</ref>

-	~~===Cloning~~, ~~expression, characterisation and three- dimensional structure determination~~ of the ~~Caenorhabditis elegans spermidine synthase===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_16226262}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[2b2c]] is a 2 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Caenorhabditis_elegans Caenorhabditis elegans]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=2B2C OCA].~~	+

	==See Also==		==See Also==
	*[[Spermidine Synthase\|Spermidine Synthase]]		*[[Spermidine Synthase\|Spermidine Synthase]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	<~~ref group="xtra">PMID:016226262<~~/~~ref~~><~~references group="xtra"~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Caenorhabditis elegans]]		[[Category: Caenorhabditis elegans]]
	[[Category: Spermidine synthase]]		[[Category: Spermidine synthase]]

OCA: Protected "2b2c" [edit=sysop:move=sysop]

OCA — Sat, 20 Oct 2012 18:06:42 GMT

Protected "2b2c" [edit=sysop:move=sysop]

←Older revision

Revision as of 18:06, 20 October 2012

OCA at 18:06, 20 October 2012

OCA — Sat, 20 Oct 2012 18:06:40 GMT

←Older revision		Revision as of 18:06, 20 October 2012
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-	{{Seed}}
	[[Image:2b2c.png\|left\|200px]]		[[Image:2b2c.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_2b2c", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_2b2c\| PDB=2b2c \| SCENE= }}		{{STRUCTURE_2b2c\| PDB=2b2c \| SCENE= }}

	===Cloning, expression, characterisation and three- dimensional structure determination of the Caenorhabditis elegans spermidine synthase===		===Cloning, expression, characterisation and three- dimensional structure determination of the Caenorhabditis elegans spermidine synthase===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_16226262}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 16226262 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_16226262}}		{{ABSTRACT_PUBMED_16226262}}

	==About this Structure==		==About this Structure==
-	~~2B2C~~ is a 2 ~~chains~~ structure ~~of sequences~~ from [http://en.wikipedia.org/wiki/Caenorhabditis_elegans Caenorhabditis elegans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2B2C OCA].	+	[[2b2c]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Caenorhabditis_elegans Caenorhabditis elegans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2B2C OCA].
		+
		+	==See Also==
		+	*[[Spermidine Synthase\|Spermidine Synthase]]

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~16226262~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:016226262</ref><references group="xtra"/>
	[[Category: Caenorhabditis elegans]]		[[Category: Caenorhabditis elegans]]
	[[Category: Spermidine synthase]]		[[Category: Spermidine synthase]]
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	[[Category: Walter, R D.]]		[[Category: Walter, R D.]]
	[[Category: Beta-alpha]]		[[Category: Beta-alpha]]
-		+	[[Category: Transferase]]
-	~~''Page seeded by~~ [~~http~~:~~//oca.weizmann.ac.il/oca OCA~~ ] ~~on Tue Feb 17 19:42:41 2009''~~	+

OCA at 17:42, 17 February 2009

OCA — Tue, 17 Feb 2009 17:42:42 GMT

←Older revision		Revision as of 17:42, 17 February 2009
Line 20:		Line 20:

	==About this Structure==		==About this Structure==
-	2B2C is a ~~[[Single protein]]~~ structure of ~~sequence~~ from [http://en.wikipedia.org/wiki/Caenorhabditis_elegans Caenorhabditis elegans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2B2C OCA].	+	2B2C is a 2 chains structure of sequences from [http://en.wikipedia.org/wiki/Caenorhabditis_elegans Caenorhabditis elegans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2B2C OCA].

	==Reference==		==Reference==
-	Cloning, expression, characterisation and three-dimensional structure determination of Caenorhabditis elegans spermidine synthase., Dufe VT, Luersen K, Eschbach ML, Haider N, Karlberg T, Walter RD, Al-Karadaghi S, FEBS Lett. 2005 Nov 7;579(27):6037-43. Epub 2005 Oct 5. PMID:~~[http:~~//~~www.ncbi.nlm.nih.gov/pubmed/16226262 16226262]~~	+	<ref group="xtra">PMID:16226262</ref><references group="xtra"/>
	[[Category: Caenorhabditis elegans]]		[[Category: Caenorhabditis elegans]]
-	[[Category: Single protein]]
	[[Category: Spermidine synthase]]		[[Category: Spermidine synthase]]
	[[Category: Al-Karadaghi, S.]]		[[Category: Al-Karadaghi, S.]]
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	[[Category: Beta-alpha]]		[[Category: Beta-alpha]]

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue ~~Jul 29 03~~:35:~~36 2008~~''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 19:42:41 2009''