2br4 - Revision history

OCA at 13:53, 13 December 2023

OCA — Wed, 13 Dec 2023 13:53:07 GMT

←Older revision		Revision as of 13:53, 13 December 2023
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	<StructureSection load='2br4' size='340' side='right'caption='[[2br4]], [[Resolution\|resolution]] 2.59Å' scene=''>		<StructureSection load='2br4' size='340' side='right'caption='[[2br4]], [[Resolution\|resolution]] 2.59Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2br4]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/~~As_4.1611 As 4.1611~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BR4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2BR4 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2br4]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_clavuligerus Streptomyces clavuligerus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BR4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2BR4 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=P4C:O-ACETALDEHYDYL-HEXAETHYLENE+GLYCOL'>P4C</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=SAM:S-ADENOSYLMETHIONINE'>SAM</scene~~></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.59Å</td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2bm8\|2bm8]], [[2bm9\|2bm9]], [[2br3\|2br3]], [[2br5\|2br5]]</div></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=P4C:O-ACETALDEHYDYL-HEXAETHYLENE+GLYCOL'>P4C</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=SAM:S-ADENOSYLMETHIONINE'>SAM</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2br4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2br4 OCA], [https://pdbe.org/2br4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2br4 RCSB], [https://www.ebi.ac.uk/pdbsum/2br4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2br4 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2br4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2br4 OCA], [https://pdbe.org/2br4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2br4 RCSB], [https://www.ebi.ac.uk/pdbsum/2br4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2br4 ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/B5GLB3_STRCL B5GLB3_STRCL] Catalyzes the O-methylation of the hydroxyl group located on C-2 of the first rhamnosyl residue linked to the phenolic group of glycosylated phenolphthiocerol dimycocerosates (PGL) and p-hydroxybenzoic acid derivatives (p-HBAD).[ARBA:ARBA00003116]
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: As 4 1611]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Andersson, I~~]]	+	[[Category: Streptomyces clavuligerus]]
-	[[Category: ~~Genereux, C~~]]	+	[[Category: Andersson I]]
-	[[Category: ~~Lester, D R~~]]	+	[[Category: Genereux C]]
-	[[Category: ~~Oster, L M~~]]	+	[[Category: Lester DR]]
-	[[Category: ~~Scheltinga, A Terwisscha van~~]]	+	[[Category: Oster LM]]
-	[[Category: Svenda, M]]	+	[[Category: Svenda M]]
-	[[Category: ~~Cephamycin biosynthesis]]~~	+	[[Category: Terwisscha van Scheltinga A]]
-	~~[[Category: Porin~~]]	+

OCA at 13:16, 24 November 2021

OCA — Wed, 24 Nov 2021 13:16:02 GMT

←Older revision		Revision as of 13:16, 24 November 2021
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	<StructureSection load='2br4' size='340' side='right'caption='[[2br4]], [[Resolution\|resolution]] 2.59Å' scene=''>		<StructureSection load='2br4' size='340' side='right'caption='[[2br4]], [[Resolution\|resolution]] 2.59Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2br4]] is a 6 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/As_4.1611 As 4.1611]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BR4 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2BR4 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2br4]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/As_4.1611 As 4.1611]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BR4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2BR4 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=P4C:O-ACETALDEHYDYL-HEXAETHYLENE+GLYCOL'>P4C</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=SAM:S-ADENOSYLMETHIONINE'>SAM</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=P4C:O-ACETALDEHYDYL-HEXAETHYLENE+GLYCOL'>P4C</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=SAM:S-ADENOSYLMETHIONINE'>SAM</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2bm8\|2bm8]], [[2bm9\|2bm9]], [[2br3\|2br3]], [[2br5\|2br5]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2bm8\|2bm8]], [[2bm9\|2bm9]], [[2br3\|2br3]], [[2br5\|2br5]]</div></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2br4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2br4 OCA], [~~http~~://pdbe.org/2br4 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2br4 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2br4 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2br4 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2br4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2br4 OCA], [https://pdbe.org/2br4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2br4 RCSB], [https://www.ebi.ac.uk/pdbsum/2br4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2br4 ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==

OCA at 08:22, 19 February 2020

OCA — Wed, 19 Feb 2020 08:22:32 GMT

←Older revision		Revision as of 08:22, 19 February 2020
Line 1:		Line 1:

	==cmcI-D160 Mg-SAM==		==cmcI-D160 Mg-SAM==
-	<StructureSection load='2br4' size='340' side='right' caption='[[2br4]], [[Resolution\|resolution]] 2.59Å' scene=''>	+	<StructureSection load='2br4' size='340' side='right'caption='[[2br4]], [[Resolution\|resolution]] 2.59Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2br4]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/As_4.1611 As 4.1611]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BR4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2BR4 FirstGlance]. <br>		<table><tr><td colspan='2'>[[2br4]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/As_4.1611 As 4.1611]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BR4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2BR4 FirstGlance]. <br>
Line 32:		Line 32:
	</StructureSection>		</StructureSection>
	[[Category: As 4 1611]]		[[Category: As 4 1611]]
		+	[[Category: Large Structures]]
	[[Category: Andersson, I]]		[[Category: Andersson, I]]
	[[Category: Genereux, C]]		[[Category: Genereux, C]]

OCA at 06:56, 16 May 2018

OCA — Wed, 16 May 2018 06:56:09 GMT

←Older revision		Revision as of 06:56, 16 May 2018
Line 1:		Line 1:
-	==~~CMCI~~-D160 MG-SAM==	+
		+	==cmcI-D160 Mg-SAM==
	<StructureSection load='2br4' size='340' side='right' caption='[[2br4]], [[Resolution\|resolution]] 2.59Å' scene=''>		<StructureSection load='2br4' size='340' side='right' caption='[[2br4]], [[Resolution\|resolution]] 2.59Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
Line 5:		Line 6:
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=P4C:O-ACETALDEHYDYL-HEXAETHYLENE+GLYCOL'>P4C</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=SAM:S-ADENOSYLMETHIONINE'>SAM</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=P4C:O-ACETALDEHYDYL-HEXAETHYLENE+GLYCOL'>P4C</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=SAM:S-ADENOSYLMETHIONINE'>SAM</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2bm8\|2bm8]], [[2bm9\|2bm9]], [[2br3\|2br3]], [[2br5\|2br5]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2bm8\|2bm8]], [[2bm9\|2bm9]], [[2br3\|2br3]], [[2br5\|2br5]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2br4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2br4 OCA], [http://pdbe.org/2br4 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2br4 RCSB], [http://www.ebi.ac.uk/pdbsum/2br4 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2br4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2br4 OCA], [http://pdbe.org/2br4 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2br4 RCSB], [http://www.ebi.ac.uk/pdbsum/2br4 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2br4 ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 11:		Line 12:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/br/2br4_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/br/2br4_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
Line 35:		Line 36:
	[[Category: Lester, D R]]		[[Category: Lester, D R]]
	[[Category: Oster, L M]]		[[Category: Oster, L M]]
-	[[Category: Scheltinga, A Terwisscha ~~Van~~]]	+	[[Category: Scheltinga, A Terwisscha van]]
	[[Category: Svenda, M]]		[[Category: Svenda, M]]
	[[Category: Cephamycin biosynthesis]]		[[Category: Cephamycin biosynthesis]]
	[[Category: Porin]]		[[Category: Porin]]

OCA at 09:58, 7 February 2016

OCA — Sun, 07 Feb 2016 09:58:05 GMT

←Older revision		Revision as of 09:58, 7 February 2016
Line 15:		Line 15:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2br4 ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 00:22, 10 September 2015

OCA — Thu, 10 Sep 2015 00:22:06 GMT

←Older revision		Revision as of 00:22, 10 September 2015
Line 2:		Line 2:
	<StructureSection load='2br4' size='340' side='right' caption='[[2br4]], [[Resolution\|resolution]] 2.59Å' scene=''>		<StructureSection load='2br4' size='340' side='right' caption='[[2br4]], [[Resolution\|resolution]] 2.59Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2br4]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Streptomyces_clavuligerus Streptomyces clavuligerus~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BR4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2BR4 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2br4]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/As_4.1611 As 4.1611]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BR4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2BR4 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=P4C:O-ACETALDEHYDYL-HEXAETHYLENE+GLYCOL'>P4C</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=SAM:S-ADENOSYLMETHIONINE'>SAM</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=P4C:O-ACETALDEHYDYL-HEXAETHYLENE+GLYCOL'>P4C</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=SAM:S-ADENOSYLMETHIONINE'>SAM</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2bm8\|2bm8]], [[2bm9\|2bm9]], [[2br3\|2br3]], [[2br5\|2br5]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2bm8\|2bm8]], [[2bm9\|2bm9]], [[2br3\|2br3]], [[2br5\|2br5]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2br4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2br4 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2br4 RCSB], [http://www.ebi.ac.uk/pdbsum/2br4 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2br4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2br4 OCA], [http://pdbe.org/2br4 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2br4 RCSB], [http://www.ebi.ac.uk/pdbsum/2br4 PDBsum]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 25:		Line 25:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 2br4" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Streptomyces clavuligerus~~]]	+	[[Category: As 4 1611]]
	[[Category: Andersson, I]]		[[Category: Andersson, I]]
	[[Category: Genereux, C]]		[[Category: Genereux, C]]

OCA at 10:46, 8 January 2015

OCA — Thu, 08 Jan 2015 10:46:01 GMT

←Older revision		Revision as of 10:46, 8 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2br4]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptomyces_clavuligerus Streptomyces clavuligerus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BR4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2BR4 FirstGlance]. <br>		<table><tr><td colspan='2'>[[2br4]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptomyces_clavuligerus Streptomyces clavuligerus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BR4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2BR4 FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=P4C:O-ACETALDEHYDYL-HEXAETHYLENE+GLYCOL'>P4C</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=SAM:S-ADENOSYLMETHIONINE'>SAM</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=P4C:O-ACETALDEHYDYL-HEXAETHYLENE+GLYCOL'>P4C</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=SAM:S-ADENOSYLMETHIONINE'>SAM</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2bm8\|2bm8]], [[2bm9\|2bm9]], [[2br3\|2br3]], [[2br5\|2br5]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2bm8\|2bm8]], [[2bm9\|2bm9]], [[2br3\|2br3]], [[2br5\|2br5]]</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2br4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2br4 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2br4 RCSB], [http://www.ebi.ac.uk/pdbsum/2br4 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2br4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2br4 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2br4 RCSB], [http://www.ebi.ac.uk/pdbsum/2br4 PDBsum]</span></td></tr>
-	<table>	+	</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 30:		Line 30:
	</StructureSection>		</StructureSection>
	[[Category: Streptomyces clavuligerus]]		[[Category: Streptomyces clavuligerus]]
-	[[Category: Andersson, I.]]	+	[[Category: Andersson, I]]
-	[[Category: Genereux, C.]]	+	[[Category: Genereux, C]]
-	[[Category: Lester, D R.]]	+	[[Category: Lester, D R]]
-	[[Category: Oster, L M.]]	+	[[Category: Oster, L M]]
-	[[Category: Scheltinga, A Terwisscha Van.]]	+	[[Category: Scheltinga, A Terwisscha Van]]
-	[[Category: Svenda, M.]]	+	[[Category: Svenda, M]]
	[[Category: Cephamycin biosynthesis]]		[[Category: Cephamycin biosynthesis]]
	[[Category: Porin]]		[[Category: Porin]]

OCA at 03:07, 30 September 2014

OCA — Tue, 30 Sep 2014 03:07:50 GMT

←Older revision		Revision as of 03:07, 30 September 2014
Line 1:		Line 1:
-	[[~~Image:~~2br4.~~png~~\|~~left~~\|200px]]	+	==CMCI-D160 MG-SAM==
		+	<StructureSection load='2br4' size='340' side='right' caption='[[2br4]], [[Resolution\|resolution]] 2.59Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[2br4]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptomyces_clavuligerus Streptomyces clavuligerus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BR4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2BR4 FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=P4C:O-ACETALDEHYDYL-HEXAETHYLENE+GLYCOL'>P4C</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=SAM:S-ADENOSYLMETHIONINE'>SAM</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2bm8\|2bm8]], [[2bm9\|2bm9]], [[2br3\|2br3]], [[2br5\|2br5]]</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2br4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2br4 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2br4 RCSB], [http://www.ebi.ac.uk/pdbsum/2br4 PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/br/2br4_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Cephamycin C-producing microorganisms use two enzymes to convert cephalosporins to their 7alpha-methoxy derivatives. Here we report the X-ray structure of one of these enzymes, CmcI, from Streptomyces clavuligerus. The polypeptide chain of the enzyme folds into a C-terminal Rossmann domain and a smaller N-terminal domain, and the molecule packs as a hexamer in the crystal. The Rossmann domain binds S-adenosyl-L-methionine (SAM) and the demethylated product, S-adenosyl-L-homocysteine, in a fashion similar to the common binding mode of this cofactor in SAM-dependent methyltransferases. There is a magnesium-binding site in the vicinity of the SAM site with a bound magnesium ion ligated by residues Asp160, Glu186 and Asp187. The expected cephalosporin binding site near the magnesium ion is occupied by polyethyleneglycol (PEG) from the crystallisation medium. The geometry of the SAM and the magnesium binding sites is similar to that found in cathechol O-methyltransferase. The results suggest CmcI is a methyltransferase, and its most likely function is to catalyse the transfer of a methyl group from SAM to the 7alpha-hydroxy cephalosporin in the second catalytic reaction of cephamycin formation. Based on the docking of the putative substrate, 7alpha-hydroxy-O-carbamoyldeacetylcephalosporin C, to the structure of the ternary CmcI-Mg2+-SAM complex, we propose a model for substrate binding and catalysis. In this model, the 7-hydroxy group of the beta-lactam ring ligates the Mg2+ with its alpha-side facing the methyl group of SAM at a distance that would allow methylation of the hydroxyl-group.

-	~~{{STRUCTURE_2br4\| PDB=2br4 \| SCENE= }}~~	+	Insights into cephamycin biosynthesis: the crystal structure of CmcI from Streptomyces clavuligerus.,Oster LM, Lester DR, Terwisscha van Scheltinga A, Svenda M, van Lun M, Genereux C, Andersson I J Mol Biol. 2006 Apr 28;358(2):546-58. Epub 2006 Feb 21. PMID:16527306<ref>PMID:16527306</ref>

-	~~===CMCI-D160 MG-SAM===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_16527306}}~~	+	== References ==
-		+	<references/>
-	~~==About this Structure==~~	+	__TOC__
-	~~[[2br4]] is~~ a ~~6 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Streptomyces_clavuligerus Streptomyces clavuligerus]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=2BR4 OCA].~~	+	</StructureSection>
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:016527306<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Streptomyces clavuligerus]]		[[Category: Streptomyces clavuligerus]]
	[[Category: Andersson, I.]]		[[Category: Andersson, I.]]

OCA: Protected "2br4" [edit=sysop:move=sysop]

OCA — Sun, 06 Jan 2013 12:17:30 GMT

Protected "2br4" [edit=sysop:move=sysop]

←Older revision

Revision as of 12:17, 6 January 2013

OCA at 12:17, 6 January 2013

OCA — Sun, 06 Jan 2013 12:17:28 GMT

←Older revision		Revision as of 12:17, 6 January 2013
Line 1:		Line 1:
-	{{Seed}}
	[[Image:2br4.png\|left\|200px]]		[[Image:2br4.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_2br4", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_2br4\| PDB=2br4 \| SCENE= }}		{{STRUCTURE_2br4\| PDB=2br4 \| SCENE= }}

	===CMCI-D160 MG-SAM===		===CMCI-D160 MG-SAM===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_16527306}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 16527306 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_16527306}}		{{ABSTRACT_PUBMED_16527306}}

	==About this Structure==		==About this Structure==
-	~~2BR4~~ is a 6 ~~chains~~ structure ~~of sequences~~ from [http://en.wikipedia.org/wiki/Streptomyces_clavuligerus Streptomyces clavuligerus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BR4 OCA].	+	[[2br4]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptomyces_clavuligerus Streptomyces clavuligerus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BR4 OCA].

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~16527306~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:016527306</ref><references group="xtra"/>
	[[Category: Streptomyces clavuligerus]]		[[Category: Streptomyces clavuligerus]]
	[[Category: Andersson, I.]]		[[Category: Andersson, I.]]
Line 33:		Line 21:
	[[Category: Cephamycin biosynthesis]]		[[Category: Cephamycin biosynthesis]]
	[[Category: Porin]]		[[Category: Porin]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Feb 16 13:02:20 2009''