2bs5 - Revision history

OCA at 06:38, 1 May 2024

OCA — Wed, 01 May 2024 06:38:21 GMT

←Older revision		Revision as of 06:38, 1 May 2024
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2bs5]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Ralstonia_solanacearum Ralstonia solanacearum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BS5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2BS5 FirstGlance]. <br>		<table><tr><td colspan='2'>[[2bs5]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Ralstonia_solanacearum Ralstonia solanacearum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BS5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2BS5 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=FUC:ALPHA-L-FUCOSE'>FUC</scene>, <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene>, <scene name='pdbligand=PRD_900070:2-fucosyllactose'>PRD_900070</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.1Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=FUC:ALPHA-L-FUCOSE'>FUC</scene>, <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene>, <scene name='pdbligand=PRD_900070:2-fucosyllactose'>PRD_900070</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2bs5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2bs5 OCA], [https://pdbe.org/2bs5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2bs5 RCSB], [https://www.ebi.ac.uk/pdbsum/2bs5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2bs5 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2bs5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2bs5 OCA], [https://pdbe.org/2bs5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2bs5 RCSB], [https://www.ebi.ac.uk/pdbsum/2bs5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2bs5 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[https://www.uniprot.org/uniprot/A0A0S4TLR1_RALSL A0A0S4TLR1_RALSL]		[https://www.uniprot.org/uniprot/A0A0S4TLR1_RALSL A0A0S4TLR1_RALSL]
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	Plant pathogens, like animal ones, use protein-carbohydrate interactions in their strategy for host recognition, attachment, and invasion. The bacterium Ralstonia solanacearum, which is distributed worldwide and causes lethal wilt in many agricultural crops, was shown to produce a potent L-fucose-binding lectin, R. solanacearum lectin, a small protein of 90 amino acids with a tandem repeat in its amino acid sequence. In the present study, surface plasmon resonance experiments conducted on a series of oligosaccharides show a preference for binding to alphaFuc1-2Gal and alphaFuc1-6Gal epitopes. Titration microcalorimetry demonstrates the presence of two binding sites per monomer and an unusually high affinity of the lectin for alphaFuc1-2Gal-containing oligosaccharides (KD = 2.5 x 10(-7) M for 2-fucosyllactose). R. solanacearum lectin has been crystallized with a methyl derivative of fucose and with the highest affinity ligand, 2-fucosyllactose. X-ray crystal structures, the one with alpha-methyl-fucoside being at ultrahigh resolution, reveal that each monomer consists of two small four-stranded anti-parallel beta-sheets. Trimerization through a 3-fold or pseudo-3-fold axis generates a six-bladed beta-propeller architecture, very similar to that previously described for the fungal lectin of Aleuria aurantia. This is the first report of a beta-propeller formed by oligomerization and not by sequential domains. Each monomer presents two fucose binding sites, resulting in six symmetrically arranged sugar binding sites for the beta-propeller. Crystals were also obtained for a mutated lectin complexed with a fragment of xyloglucan, a fucosylated polysaccharide from the primary cell wall of plants, which may be the biological target of the lectin.
-
-	The fucose-binding lectin from Ralstonia solanacearum. A new type of beta-propeller architecture formed by oligomerization and interacting with fucoside, fucosyllactose, and plant xyloglucan.,Kostlanova N, Mitchell EP, Lortat-Jacob H, Oscarson S, Lahmann M, Gilboa-Garber N, Chambat G, Wimmerova M, Imberty A J Biol Chem. 2005 Jul 29;280(30):27839-49. Epub 2005 May 27. PMID:15923179<ref>PMID:15923179</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 2bs5" style="background-color:#fffaf0;"></div>
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 11:21, 30 November 2022

OCA — Wed, 30 Nov 2022 11:21:20 GMT

←Older revision		Revision as of 11:21, 30 November 2022
Line 3:		Line 3:
	<StructureSection load='2bs5' size='340' side='right'caption='[[2bs5]], [[Resolution\|resolution]] 2.10Å' scene=''>		<StructureSection load='2bs5' size='340' side='right'caption='[[2bs5]], [[Resolution\|resolution]] 2.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2bs5]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~"bacillus_solanacearum"_smith_1896 "bacillus~~ solanacearum~~" smith 1896~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BS5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2BS5 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2bs5]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Ralstonia_solanacearum Ralstonia solanacearum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BS5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2BS5 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=FUC:ALPHA-L-FUCOSE'>FUC</scene>, <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=FUC:ALPHA-L-FUCOSE'>FUC</scene>, <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene>, <scene name='pdbligand=PRD_900070:2-fucosyllactose'>PRD_900070</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2bs5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2bs5 OCA], [https://pdbe.org/2bs5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2bs5 RCSB], [https://www.ebi.ac.uk/pdbsum/2bs5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2bs5 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2bs5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2bs5 OCA], [https://pdbe.org/2bs5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2bs5 RCSB], [https://www.ebi.ac.uk/pdbsum/2bs5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2bs5 ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/A0A0S4TLR1_RALSL A0A0S4TLR1_RALSL]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 20:		Line 22:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Bacillus solanacearum smith 1896]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Imberty, A]]	+	[[Category: Ralstonia solanacearum]]
-	[[Category: Kostlanova, N]]	+	[[Category: Imberty A]]
-	[[Category: Mitchell~~, E P~~]]	+	[[Category: Kostlanova N]]
-	[[Category: Wimmerova, M]]	+	[[Category: Mitchell EP]]
-	~~[[Category: Beta-propeller]]~~	+	[[Category: Wimmerova M]]
-	~~[[Category: Sugar binding protein]]~~	+
-	~~[[Category: Sugar recognition~~]]	+

OCA at 09:17, 24 February 2021

OCA — Wed, 24 Feb 2021 09:17:18 GMT

←Older revision		Revision as of 09:17, 24 February 2021
Line 1:		Line 1:

-	====	+	==LECTIN FROM RALSTONIA SOLANACEARUM COMPLEXED WITH 2-FUCOSYLLACTOSE==
-	<StructureSection load='2bs5' size='340' side='right'caption='[[2bs5]]' scene=''>	+	<StructureSection load='2bs5' size='340' side='right'caption='[[2bs5]], [[Resolution\|resolution]] 2.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2bs5]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/"bacillus_solanacearum"_smith_1896 "bacillus solanacearum" smith 1896]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BS5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2BS5 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2bs5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2bs5 OCA], [https://pdbe.org/2bs5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2bs5 RCSB], [https://www.ebi.ac.uk/pdbsum/2bs5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2bs5 ProSAT]</span></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=FUC:ALPHA-L-FUCOSE'>FUC</scene>, <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2bs5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2bs5 OCA], [https://pdbe.org/2bs5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2bs5 RCSB], [https://www.ebi.ac.uk/pdbsum/2bs5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2bs5 ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Plant pathogens, like animal ones, use protein-carbohydrate interactions in their strategy for host recognition, attachment, and invasion. The bacterium Ralstonia solanacearum, which is distributed worldwide and causes lethal wilt in many agricultural crops, was shown to produce a potent L-fucose-binding lectin, R. solanacearum lectin, a small protein of 90 amino acids with a tandem repeat in its amino acid sequence. In the present study, surface plasmon resonance experiments conducted on a series of oligosaccharides show a preference for binding to alphaFuc1-2Gal and alphaFuc1-6Gal epitopes. Titration microcalorimetry demonstrates the presence of two binding sites per monomer and an unusually high affinity of the lectin for alphaFuc1-2Gal-containing oligosaccharides (KD = 2.5 x 10(-7) M for 2-fucosyllactose). R. solanacearum lectin has been crystallized with a methyl derivative of fucose and with the highest affinity ligand, 2-fucosyllactose. X-ray crystal structures, the one with alpha-methyl-fucoside being at ultrahigh resolution, reveal that each monomer consists of two small four-stranded anti-parallel beta-sheets. Trimerization through a 3-fold or pseudo-3-fold axis generates a six-bladed beta-propeller architecture, very similar to that previously described for the fungal lectin of Aleuria aurantia. This is the first report of a beta-propeller formed by oligomerization and not by sequential domains. Each monomer presents two fucose binding sites, resulting in six symmetrically arranged sugar binding sites for the beta-propeller. Crystals were also obtained for a mutated lectin complexed with a fragment of xyloglucan, a fucosylated polysaccharide from the primary cell wall of plants, which may be the biological target of the lectin.
		+
		+	The fucose-binding lectin from Ralstonia solanacearum. A new type of beta-propeller architecture formed by oligomerization and interacting with fucoside, fucosyllactose, and plant xyloglucan.,Kostlanova N, Mitchell EP, Lortat-Jacob H, Oscarson S, Lahmann M, Gilboa-Garber N, Chambat G, Wimmerova M, Imberty A J Biol Chem. 2005 Jul 29;280(30):27839-49. Epub 2005 May 27. PMID:15923179<ref>PMID:15923179</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 2bs5" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Bacillus solanacearum smith 1896]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Z-~~disk~~]]	+	[[Category: Imberty, A]]
		+	[[Category: Kostlanova, N]]
		+	[[Category: Mitchell, E P]]
		+	[[Category: Wimmerova, M]]
		+	[[Category: Beta-propeller]]
		+	[[Category: Sugar binding protein]]
		+	[[Category: Sugar recognition]]

OCA at 07:10, 24 February 2021

OCA — Wed, 24 Feb 2021 07:10:43 GMT

←Older revision		Revision as of 07:10, 24 February 2021
Line 1:		Line 1:

-	==~~LECTIN FROM RALSTONIA SOLANACEARUM COMPLEXED WITH 2-FUCOSYLLACTOSE~~==	+	====
-	<StructureSection load='2bs5' size='340' side='right'caption='[[2bs5]]~~, [[Resolution\|resolution]] 2.10Å~~' scene=''>	+	<StructureSection load='2bs5' size='340' side='right'caption='[[2bs5]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>~~[[2bs5]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_solanacearum"_smith_1896 "bacillus solanacearum" smith 1896].~~ Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=~~2BS5~~ OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=~~2BS5~~ FirstGlance]. <br>	+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=FUC:ALPHA-L-FUCOSE'>FUC</scene>, <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2bs5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2bs5 OCA], [https://pdbe.org/2bs5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2bs5 RCSB], [https://www.ebi.ac.uk/pdbsum/2bs5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2bs5 ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2bs5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2bs5 OCA], [~~http~~://pdbe.org/2bs5 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2bs5 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2bs5 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2bs5 ProSAT]</span></td></tr>	+
	</table>		</table>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	Plant pathogens, like animal ones, use protein-carbohydrate interactions in their strategy for host recognition, attachment, and invasion. The bacterium Ralstonia solanacearum, which is distributed worldwide and causes lethal wilt in many agricultural crops, was shown to produce a potent L-fucose-binding lectin, R. solanacearum lectin, a small protein of 90 amino acids with a tandem repeat in its amino acid sequence. In the present study, surface plasmon resonance experiments conducted on a series of oligosaccharides show a preference for binding to alphaFuc1-2Gal and alphaFuc1-6Gal epitopes. Titration microcalorimetry demonstrates the presence of two binding sites per monomer and an unusually high affinity of the lectin for alphaFuc1-2Gal-containing oligosaccharides (KD = 2.5 x 10(-7) M for 2-fucosyllactose). R. solanacearum lectin has been crystallized with a methyl derivative of fucose and with the highest affinity ligand, 2-fucosyllactose. X-ray crystal structures, the one with alpha-methyl-fucoside being at ultrahigh resolution, reveal that each monomer consists of two small four-stranded anti-parallel beta-sheets. Trimerization through a 3-fold or pseudo-3-fold axis generates a six-bladed beta-propeller architecture, very similar to that previously described for the fungal lectin of Aleuria aurantia. This is the first report of a beta-propeller formed by oligomerization and not by sequential domains. Each monomer presents two fucose binding sites, resulting in six symmetrically arranged sugar binding sites for the beta-propeller. Crystals were also obtained for a mutated lectin complexed with a fragment of xyloglucan, a fucosylated polysaccharide from the primary cell wall of plants, which may be the biological target of the lectin.
-
-	The fucose-binding lectin from Ralstonia solanacearum. A new type of beta-propeller architecture formed by oligomerization and interacting with fucoside, fucosyllactose, and plant xyloglucan.,Kostlanova N, Mitchell EP, Lortat-Jacob H, Oscarson S, Lahmann M, Gilboa-Garber N, Chambat G, Wimmerova M, Imberty A J Biol Chem. 2005 Jul 29;280(30):27839-49. Epub 2005 May 27. PMID:15923179<ref>PMID:15923179</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 2bs5" style="background-color:#fffaf0;"></div>
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Bacillus solanacearum smith 1896]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Imberty, A]]~~	+	[[Category: Z-disk]]
-	~~[[Category: Kostlanova, N]]~~	+
-	~~[[Category: Mitchell, E P]]~~	+
-	~~[[Category: Wimmerova, M]]~~	+
-	~~[[Category: Beta~~-~~propeller]]~~	+
-	~~[[Category: Sugar binding protein]]~~	+
-	~~[[Category: Sugar recognition~~]]	+

OCA at 11:52, 10 May 2019

OCA — Fri, 10 May 2019 11:52:14 GMT

←Older revision		Revision as of 11:52, 10 May 2019
Line 1:		Line 1:

	==LECTIN FROM RALSTONIA SOLANACEARUM COMPLEXED WITH 2-FUCOSYLLACTOSE==		==LECTIN FROM RALSTONIA SOLANACEARUM COMPLEXED WITH 2-FUCOSYLLACTOSE==
-	<StructureSection load='2bs5' size='340' side='right' caption='[[2bs5]], [[Resolution\|resolution]] 2.10Å' scene=''>	+	<StructureSection load='2bs5' size='340' side='right'caption='[[2bs5]], [[Resolution\|resolution]] 2.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2bs5]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_solanacearum"_smith_1896 "bacillus solanacearum" smith 1896]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BS5 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2BS5 FirstGlance]. <br>		<table><tr><td colspan='2'>[[2bs5]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_solanacearum"_smith_1896 "bacillus solanacearum" smith 1896]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BS5 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2BS5 FirstGlance]. <br>
Line 21:		Line 21:
	</StructureSection>		</StructureSection>
	[[Category: Bacillus solanacearum smith 1896]]		[[Category: Bacillus solanacearum smith 1896]]
		+	[[Category: Large Structures]]
	[[Category: Imberty, A]]		[[Category: Imberty, A]]
	[[Category: Kostlanova, N]]		[[Category: Kostlanova, N]]

OCA at 06:38, 16 May 2018

OCA — Wed, 16 May 2018 06:38:23 GMT

←Older revision		Revision as of 06:38, 16 May 2018
Line 1:		Line 1:
		+
	==LECTIN FROM RALSTONIA SOLANACEARUM COMPLEXED WITH 2-FUCOSYLLACTOSE==		==LECTIN FROM RALSTONIA SOLANACEARUM COMPLEXED WITH 2-FUCOSYLLACTOSE==
	<StructureSection load='2bs5' size='340' side='right' caption='[[2bs5]], [[Resolution\|resolution]] 2.10Å' scene=''>		<StructureSection load='2bs5' size='340' side='right' caption='[[2bs5]], [[Resolution\|resolution]] 2.10Å' scene=''>
Line 4:		Line 5:
	<table><tr><td colspan='2'>[[2bs5]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_solanacearum"_smith_1896 "bacillus solanacearum" smith 1896]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BS5 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2BS5 FirstGlance]. <br>		<table><tr><td colspan='2'>[[2bs5]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_solanacearum"_smith_1896 "bacillus solanacearum" smith 1896]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BS5 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2BS5 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=FUC:ALPHA-L-FUCOSE'>FUC</scene>, <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=FUC:ALPHA-L-FUCOSE'>FUC</scene>, <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2bs5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2bs5 OCA], [http://pdbe.org/2bs5 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2bs5 RCSB], [http://www.ebi.ac.uk/pdbsum/2bs5 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2bs5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2bs5 OCA], [http://pdbe.org/2bs5 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2bs5 RCSB], [http://www.ebi.ac.uk/pdbsum/2bs5 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2bs5 ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 15:24, 11 September 2015

OCA — Fri, 11 Sep 2015 15:24:58 GMT

←Older revision		Revision as of 15:24, 11 September 2015
Line 2:		Line 2:
	<StructureSection load='2bs5' size='340' side='right' caption='[[2bs5]], [[Resolution\|resolution]] 2.10Å' scene=''>		<StructureSection load='2bs5' size='340' side='right' caption='[[2bs5]], [[Resolution\|resolution]] 2.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2bs5]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Ralstonia_solanacearum Ralstonia~~ solanacearum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BS5 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2BS5 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2bs5]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_solanacearum"_smith_1896 "bacillus solanacearum" smith 1896]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BS5 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2BS5 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=FUC:ALPHA-L-FUCOSE'>FUC</scene>, <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=FUC:ALPHA-L-FUCOSE'>FUC</scene>, <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2bs5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2bs5 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2bs5 RCSB], [http://www.ebi.ac.uk/pdbsum/2bs5 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2bs5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2bs5 OCA], [http://pdbe.org/2bs5 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2bs5 RCSB], [http://www.ebi.ac.uk/pdbsum/2bs5 PDBsum]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 14:		Line 14:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 2bs5" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Ralstonia~~ solanacearum]]	+	[[Category: Bacillus solanacearum smith 1896]]
	[[Category: Imberty, A]]		[[Category: Imberty, A]]
	[[Category: Kostlanova, N]]		[[Category: Kostlanova, N]]

OCA at 10:32, 25 January 2015

OCA — Sun, 25 Jan 2015 10:32:42 GMT

←Older revision		Revision as of 10:32, 25 January 2015
Line 19:		Line 19:
	</StructureSection>		</StructureSection>
	[[Category: Ralstonia solanacearum]]		[[Category: Ralstonia solanacearum]]
-	[[Category: Imberty, A.]]	+	[[Category: Imberty, A]]
-	[[Category: Kostlanova, N.]]	+	[[Category: Kostlanova, N]]
-	[[Category: Mitchell, E P.]]	+	[[Category: Mitchell, E P]]
-	[[Category: Wimmerova, M.]]	+	[[Category: Wimmerova, M]]
	[[Category: Beta-propeller]]		[[Category: Beta-propeller]]
	[[Category: Sugar binding protein]]		[[Category: Sugar binding protein]]
	[[Category: Sugar recognition]]		[[Category: Sugar recognition]]

OCA at 11:53, 29 October 2014

OCA — Wed, 29 Oct 2014 11:53:00 GMT

←Older revision		Revision as of 11:53, 29 October 2014
Line 1:		Line 1:
-	[[~~Image:~~2bs5.~~png~~\|~~left\|200px~~]]	+	==LECTIN FROM RALSTONIA SOLANACEARUM COMPLEXED WITH 2-FUCOSYLLACTOSE==
		+	<StructureSection load='2bs5' size='340' side='right' caption='[[2bs5]], [[Resolution\|resolution]] 2.10Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[2bs5]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Ralstonia_solanacearum Ralstonia solanacearum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BS5 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2BS5 FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=FUC:ALPHA-L-FUCOSE'>FUC</scene>, <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2bs5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2bs5 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2bs5 RCSB], [http://www.ebi.ac.uk/pdbsum/2bs5 PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Plant pathogens, like animal ones, use protein-carbohydrate interactions in their strategy for host recognition, attachment, and invasion. The bacterium Ralstonia solanacearum, which is distributed worldwide and causes lethal wilt in many agricultural crops, was shown to produce a potent L-fucose-binding lectin, R. solanacearum lectin, a small protein of 90 amino acids with a tandem repeat in its amino acid sequence. In the present study, surface plasmon resonance experiments conducted on a series of oligosaccharides show a preference for binding to alphaFuc1-2Gal and alphaFuc1-6Gal epitopes. Titration microcalorimetry demonstrates the presence of two binding sites per monomer and an unusually high affinity of the lectin for alphaFuc1-2Gal-containing oligosaccharides (KD = 2.5 x 10(-7) M for 2-fucosyllactose). R. solanacearum lectin has been crystallized with a methyl derivative of fucose and with the highest affinity ligand, 2-fucosyllactose. X-ray crystal structures, the one with alpha-methyl-fucoside being at ultrahigh resolution, reveal that each monomer consists of two small four-stranded anti-parallel beta-sheets. Trimerization through a 3-fold or pseudo-3-fold axis generates a six-bladed beta-propeller architecture, very similar to that previously described for the fungal lectin of Aleuria aurantia. This is the first report of a beta-propeller formed by oligomerization and not by sequential domains. Each monomer presents two fucose binding sites, resulting in six symmetrically arranged sugar binding sites for the beta-propeller. Crystals were also obtained for a mutated lectin complexed with a fragment of xyloglucan, a fucosylated polysaccharide from the primary cell wall of plants, which may be the biological target of the lectin.

-	~~{{STRUCTURE_2bs5\| PDB=2bs5 \| SCENE= }}~~	+	The fucose-binding lectin from Ralstonia solanacearum. A new type of beta-propeller architecture formed by oligomerization and interacting with fucoside, fucosyllactose, and plant xyloglucan.,Kostlanova N, Mitchell EP, Lortat-Jacob H, Oscarson S, Lahmann M, Gilboa-Garber N, Chambat G, Wimmerova M, Imberty A J Biol Chem. 2005 Jul 29;280(30):27839-49. Epub 2005 May 27. PMID:15923179<ref>PMID:15923179</ref>

-	~~===LECTIN FROM RALSTONIA SOLANACEARUM COMPLEXED WITH 2-FUCOSYLLACTOSE===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_15923179}}~~	+	== References ==
-		+	<references/>
-	~~==About this Structure==~~	+	__TOC__
-	~~[[2bs5]] is~~ a ~~1 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Ralstonia_solanacearum Ralstonia solanacearum]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=2BS5 OCA].~~	+	</StructureSection>
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:015923179<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Ralstonia solanacearum]]		[[Category: Ralstonia solanacearum]]
	[[Category: Imberty, A.]]		[[Category: Imberty, A.]]

OCA at 12:16, 23 January 2013

OCA — Wed, 23 Jan 2013 12:16:45 GMT

←Older revision		Revision as of 12:16, 23 January 2013
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	[[Image:2bs5.png\|left\|200px]]		[[Image:2bs5.png\|left\|200px]]

-	<!--
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-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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	{{STRUCTURE_2bs5\| PDB=2bs5 \| SCENE= }}		{{STRUCTURE_2bs5\| PDB=2bs5 \| SCENE= }}

	===LECTIN FROM RALSTONIA SOLANACEARUM COMPLEXED WITH 2-FUCOSYLLACTOSE===		===LECTIN FROM RALSTONIA SOLANACEARUM COMPLEXED WITH 2-FUCOSYLLACTOSE===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_15923179}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 15923179 is the PubMed ID number.
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	{{ABSTRACT_PUBMED_15923179}}		{{ABSTRACT_PUBMED_15923179}}

Line 22:		Line 11:

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~15923179~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:015923179</ref><references group="xtra"/>
	[[Category: Ralstonia solanacearum]]		[[Category: Ralstonia solanacearum]]
	[[Category: Imberty, A.]]		[[Category: Imberty, A.]]
Line 28:		Line 17:
	[[Category: Mitchell, E P.]]		[[Category: Mitchell, E P.]]
	[[Category: Wimmerova, M.]]		[[Category: Wimmerova, M.]]
		+	[[Category: Beta-propeller]]
		+	[[Category: Sugar binding protein]]
		+	[[Category: Sugar recognition]]