2bz3 - Revision history

OCA at 13:59, 13 December 2023

OCA — Wed, 13 Dec 2023 13:59:26 GMT

←Older revision		Revision as of 13:59, 13 December 2023
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	<StructureSection load='2bz3' size='340' side='right'caption='[[2bz3]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='2bz3' size='340' side='right'caption='[[2bz3]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2bz3]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/~~"bacillus_coli"_migula_1895 "bacillus~~ coli~~" migula 1895~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BZ3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2BZ3 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2bz3]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BZ3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2BZ3 FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=DAO:LAURIC+ACID'>DAO</scene>~~, ~~<scene name='pdbligand=NH4:AMMONIUM+ION'>NH4</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2Å</td></tr>
-	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"><~~div style~~='~~overflow~~: ~~auto; max-height: 3em;~~'>[[1dd8\|1dd8]], [[1ek4\|1ek4]], [[1f91\|1f91]], [[1fj4\|1fj4]], [[1fj8\|1fj8]], [[1g5x\|1g5x]], [[1h4f\|1h4f]], [[2buh\|2buh]], [[2bui\|2bui]], [[2byw\|2byw]], [[2byx\|2byx]], [[2byy\|2byy]], [[2byz\|2byz]], [[2bz4\|2bz4]]</~~div~~><~~/td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DAO:LAURIC+ACID'>DAO</scene>, <scene name='pdbligand=NH4:AMMONIUM+ION'>NH4</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	~~<tr id~~='~~activity'><td class~~=~~"sblockLbl"><b>Activity~~:~~</b~~></td><~~td class~~=~~"sblockDat"><span class~~=~~'plainlinks~~'>[https://en.wikipedia.org/wiki/Beta-ketoacyl-[acyl-carrier-protein]_synthase_I Beta-ketoacyl-[acyl-carrier-protein] synthase I], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.3.1.41 2.3.1.41] </~~span~~></td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2bz3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2bz3 OCA], [https://pdbe.org/2bz3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2bz3 RCSB], [https://www.ebi.ac.uk/pdbsum/2bz3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2bz3 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2bz3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2bz3 OCA], [https://pdbe.org/2bz3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2bz3 RCSB], [https://www.ebi.ac.uk/pdbsum/2bz3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2bz3 ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/FABB_ECOLI FABB_ECOLI] Catalyzes the condensation reaction of fatty acid synthesis by the addition to an acyl acceptor of two carbons from malonyl-ACP. Specific for elongation from C-10 to unsaturated C-16 and C-18 fatty acids.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Bacillus~~ coli ~~migula 1895~~]]	+	[[Category: Escherichia coli]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Henriksen, A]]	+	[[Category: Henriksen A]]
-	[[Category: Olsen~~, J G~~]]	+	[[Category: Olsen JG]]
-	[[Category: Wettstein-Knowles, P ~~von]]~~	+	[[Category: Von Wettstein-Knowles P]]
-	~~[[Category: Acyltransferase]]~~	+
-	~~[[Category: Claisen condensation]]~~	+
-	~~[[Category: Fatty acid biosynthesis]]~~	+
-	~~[[Category: Fatty acid synthase]]~~	+
-	~~[[Category: Lipid synthesis]]~~	+
-	~~[[Category: Thiolase fold]]~~	+
-	~~[[Category: Transferase~~]]	+

OCA at 13:40, 13 October 2021

OCA — Wed, 13 Oct 2021 13:40:52 GMT

←Older revision		Revision as of 13:40, 13 October 2021
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	<StructureSection load='2bz3' size='340' side='right'caption='[[2bz3]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='2bz3' size='340' side='right'caption='[[2bz3]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2bz3]] is a 4 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BZ3 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2BZ3 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2bz3]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BZ3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2BZ3 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=DAO:LAURIC+ACID'>DAO</scene>, <scene name='pdbligand=NH4:AMMONIUM+ION'>NH4</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DAO:LAURIC+ACID'>DAO</scene>, <scene name='pdbligand=NH4:AMMONIUM+ION'>NH4</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1dd8\|1dd8]], [[1ek4\|1ek4]], [[1f91\|1f91]], [[1fj4\|1fj4]], [[1fj8\|1fj8]], [[1g5x\|1g5x]], [[1h4f\|1h4f]], [[2buh\|2buh]], [[2bui\|2bui]], [[2byw\|2byw]], [[2byx\|2byx]], [[2byy\|2byy]], [[2byz\|2byz]], [[2bz4\|2bz4]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1dd8\|1dd8]], [[1ek4\|1ek4]], [[1f91\|1f91]], [[1fj4\|1fj4]], [[1fj8\|1fj8]], [[1g5x\|1g5x]], [[1h4f\|1h4f]], [[2buh\|2buh]], [[2bui\|2bui]], [[2byw\|2byw]], [[2byx\|2byx]], [[2byy\|2byy]], [[2byz\|2byz]], [[2bz4\|2bz4]]</div></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Beta-ketoacyl-[acyl-carrier-protein]_synthase_I Beta-ketoacyl-[acyl-carrier-protein] synthase I], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.3.1.41 2.3.1.41] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Beta-ketoacyl-[acyl-carrier-protein]_synthase_I Beta-ketoacyl-[acyl-carrier-protein] synthase I], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.3.1.41 2.3.1.41] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2bz3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2bz3 OCA], [~~http~~://pdbe.org/2bz3 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2bz3 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2bz3 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2bz3 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2bz3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2bz3 OCA], [https://pdbe.org/2bz3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2bz3 RCSB], [https://www.ebi.ac.uk/pdbsum/2bz3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2bz3 ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==

OCA at 19:03, 11 December 2019

OCA — Wed, 11 Dec 2019 19:03:13 GMT

←Older revision		Revision as of 19:03, 11 December 2019
Line 1:		Line 1:

	==Structure of E.coli KAS I H298E mutant in complex with C12 fatty acid==		==Structure of E.coli KAS I H298E mutant in complex with C12 fatty acid==
-	<StructureSection load='2bz3' size='340' side='right' caption='[[2bz3]], [[Resolution\|resolution]] 2.00Å' scene=''>	+	<StructureSection load='2bz3' size='340' side='right'caption='[[2bz3]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2bz3]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BZ3 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2BZ3 FirstGlance]. <br>		<table><tr><td colspan='2'>[[2bz3]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BZ3 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2BZ3 FirstGlance]. <br>
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	</div>		</div>
	<div class="pdbe-citations 2bz3" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 2bz3" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Acyl carrier protein synthase 3D structures\|Acyl carrier protein synthase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
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	</StructureSection>		</StructureSection>
	[[Category: Bacillus coli migula 1895]]		[[Category: Bacillus coli migula 1895]]
		+	[[Category: Large Structures]]
	[[Category: Henriksen, A]]		[[Category: Henriksen, A]]
	[[Category: Olsen, J G]]		[[Category: Olsen, J G]]

OCA at 07:19, 17 January 2018

OCA — Wed, 17 Jan 2018 07:19:48 GMT

←Older revision		Revision as of 07:19, 17 January 2018
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		+
	==Structure of E.coli KAS I H298E mutant in complex with C12 fatty acid==		==Structure of E.coli KAS I H298E mutant in complex with C12 fatty acid==
	<StructureSection load='2bz3' size='340' side='right' caption='[[2bz3]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='2bz3' size='340' side='right' caption='[[2bz3]], [[Resolution\|resolution]] 2.00Å' scene=''>
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	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1dd8\|1dd8]], [[1ek4\|1ek4]], [[1f91\|1f91]], [[1fj4\|1fj4]], [[1fj8\|1fj8]], [[1g5x\|1g5x]], [[1h4f\|1h4f]], [[2buh\|2buh]], [[2bui\|2bui]], [[2byw\|2byw]], [[2byx\|2byx]], [[2byy\|2byy]], [[2byz\|2byz]], [[2bz4\|2bz4]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1dd8\|1dd8]], [[1ek4\|1ek4]], [[1f91\|1f91]], [[1fj4\|1fj4]], [[1fj8\|1fj8]], [[1g5x\|1g5x]], [[1h4f\|1h4f]], [[2buh\|2buh]], [[2bui\|2bui]], [[2byw\|2byw]], [[2byx\|2byx]], [[2byy\|2byy]], [[2byz\|2byz]], [[2bz4\|2bz4]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Beta-ketoacyl-[acyl-carrier-protein]_synthase_I Beta-ketoacyl-[acyl-carrier-protein] synthase I], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.3.1.41 2.3.1.41] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Beta-ketoacyl-[acyl-carrier-protein]_synthase_I Beta-ketoacyl-[acyl-carrier-protein] synthase I], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.3.1.41 2.3.1.41] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2bz3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2bz3 OCA], [http://pdbe.org/2bz3 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2bz3 RCSB], [http://www.ebi.ac.uk/pdbsum/2bz3 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2bz3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2bz3 OCA], [http://pdbe.org/2bz3 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2bz3 RCSB], [http://www.ebi.ac.uk/pdbsum/2bz3 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2bz3 ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
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	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/bz/2bz3_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/bz/2bz3_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
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	</div>		</div>
	<div class="pdbe-citations 2bz3" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 2bz3" style="background-color:#fffaf0;"></div>
-
-	==See Also==
-	*[[Acyl carrier protein synthase\|Acyl carrier protein synthase]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 10:34, 8 February 2016

OCA — Mon, 08 Feb 2016 10:34:52 GMT

←Older revision		Revision as of 10:34, 8 February 2016
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	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2bz3 ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 10:42, 10 September 2015

OCA — Thu, 10 Sep 2015 10:42:41 GMT

←Older revision		Revision as of 10:42, 10 September 2015
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	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1dd8\|1dd8]], [[1ek4\|1ek4]], [[1f91\|1f91]], [[1fj4\|1fj4]], [[1fj8\|1fj8]], [[1g5x\|1g5x]], [[1h4f\|1h4f]], [[2buh\|2buh]], [[2bui\|2bui]], [[2byw\|2byw]], [[2byx\|2byx]], [[2byy\|2byy]], [[2byz\|2byz]], [[2bz4\|2bz4]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1dd8\|1dd8]], [[1ek4\|1ek4]], [[1f91\|1f91]], [[1fj4\|1fj4]], [[1fj8\|1fj8]], [[1g5x\|1g5x]], [[1h4f\|1h4f]], [[2buh\|2buh]], [[2bui\|2bui]], [[2byw\|2byw]], [[2byx\|2byx]], [[2byy\|2byy]], [[2byz\|2byz]], [[2bz4\|2bz4]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Beta-ketoacyl-[acyl-carrier-protein]_synthase_I Beta-ketoacyl-[acyl-carrier-protein] synthase I], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.3.1.41 2.3.1.41] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Beta-ketoacyl-[acyl-carrier-protein]_synthase_I Beta-ketoacyl-[acyl-carrier-protein] synthase I], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.3.1.41 2.3.1.41] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2bz3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2bz3 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2bz3 RCSB], [http://www.ebi.ac.uk/pdbsum/2bz3 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2bz3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2bz3 OCA], [http://pdbe.org/2bz3 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2bz3 RCSB], [http://www.ebi.ac.uk/pdbsum/2bz3 PDBsum]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
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	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 2bz3" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Acyl carrier protein synthase\|Acyl carrier protein synthase]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 17:23, 15 January 2015

OCA — Thu, 15 Jan 2015 17:23:33 GMT

←Older revision		Revision as of 17:23, 15 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2bz3]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BZ3 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2BZ3 FirstGlance]. <br>		<table><tr><td colspan='2'>[[2bz3]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BZ3 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2BZ3 FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=DAO:LAURIC+ACID'>DAO</scene>, <scene name='pdbligand=NH4:AMMONIUM+ION'>NH4</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=DAO:LAURIC+ACID'>DAO</scene>, <scene name='pdbligand=NH4:AMMONIUM+ION'>NH4</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1dd8\|1dd8]], [[1ek4\|1ek4]], [[1f91\|1f91]], [[1fj4\|1fj4]], [[1fj8\|1fj8]], [[1g5x\|1g5x]], [[1h4f\|1h4f]], [[2buh\|2buh]], [[2bui\|2bui]], [[2byw\|2byw]], [[2byx\|2byx]], [[2byy\|2byy]], [[2byz\|2byz]], [[2bz4\|2bz4]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1dd8\|1dd8]], [[1ek4\|1ek4]], [[1f91\|1f91]], [[1fj4\|1fj4]], [[1fj8\|1fj8]], [[1g5x\|1g5x]], [[1h4f\|1h4f]], [[2buh\|2buh]], [[2bui\|2bui]], [[2byw\|2byw]], [[2byx\|2byx]], [[2byy\|2byy]], [[2byz\|2byz]], [[2bz4\|2bz4]]</td></tr>
-	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Beta-ketoacyl-[acyl-carrier-protein]_synthase_I Beta-ketoacyl-[acyl-carrier-protein] synthase I], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.3.1.41 2.3.1.41] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Beta-ketoacyl-[acyl-carrier-protein]_synthase_I Beta-ketoacyl-[acyl-carrier-protein] synthase I], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.3.1.41 2.3.1.41] </span></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2bz3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2bz3 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2bz3 RCSB], [http://www.ebi.ac.uk/pdbsum/2bz3 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2bz3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2bz3 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2bz3 RCSB], [http://www.ebi.ac.uk/pdbsum/2bz3 PDBsum]</span></td></tr>
-	<table>	+	</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 26:		Line 26:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
-
-	==See Also==
-	*[[Acyl carrier protein synthase\|Acyl carrier protein synthase]]
	== References ==		== References ==
	<references/>		<references/>
Line 34:		Line 31:
	</StructureSection>		</StructureSection>
	[[Category: Bacillus coli migula 1895]]		[[Category: Bacillus coli migula 1895]]
-	[[Category: Henriksen, A.]]	+	[[Category: Henriksen, A]]
-	[[Category: Olsen, J G.]]	+	[[Category: Olsen, J G]]
-	[[Category: Wettstein-Knowles, P von.]]	+	[[Category: Wettstein-Knowles, P von]]
	[[Category: Acyltransferase]]		[[Category: Acyltransferase]]
	[[Category: Claisen condensation]]		[[Category: Claisen condensation]]

OCA at 08:45, 3 October 2014

OCA — Fri, 03 Oct 2014 08:45:54 GMT

←Older revision		Revision as of 08:45, 3 October 2014
Line 1:		Line 1:
-	~~{{STRUCTURE_2bz3\| PDB=2bz3 \| SCENE= }}~~	+	==Structure of E.coli KAS I H298E mutant in complex with C12 fatty acid==
-	===Structure of E.coli KAS I H298E mutant in complex with C12 fatty acid===	+	<StructureSection load='2bz3' size='340' side='right' caption='[[2bz3]], [[Resolution\|resolution]] 2.00Å' scene=''>
-	~~{{ABSTRACT_PUBMED_16441657}}~~	+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[2bz3]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BZ3 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2BZ3 FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=DAO:LAURIC+ACID'>DAO</scene>, <scene name='pdbligand=NH4:AMMONIUM+ION'>NH4</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1dd8\|1dd8]], [[1ek4\|1ek4]], [[1f91\|1f91]], [[1fj4\|1fj4]], [[1fj8\|1fj8]], [[1g5x\|1g5x]], [[1h4f\|1h4f]], [[2buh\|2buh]], [[2bui\|2bui]], [[2byw\|2byw]], [[2byx\|2byx]], [[2byy\|2byy]], [[2byz\|2byz]], [[2bz4\|2bz4]]</td></tr>
		+	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Beta-ketoacyl-[acyl-carrier-protein]_synthase_I Beta-ketoacyl-[acyl-carrier-protein] synthase I], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.3.1.41 2.3.1.41] </span></td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2bz3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2bz3 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2bz3 RCSB], [http://www.ebi.ac.uk/pdbsum/2bz3 PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/bz/2bz3_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Beta-ketoacyl-acyl carrier protein (ACP) synthase enzymes join short carbon units to construct fatty acyl chains by a three-step Claisen condensation reaction. The reaction starts with a trans thioesterification of the acyl primer substrate from ACP to the enzyme. Subsequently, the donor substrate malonyl-ACP is decarboxylated to form a carbanion intermediate, which in the third step attacks C1 of the primer substrate giving rise to an elongated acyl chain. A subgroup of beta-ketoacyl-ACP synthases, including mitochondrial beta-ketoacyl-ACP synthase, bacterial plus plastid beta-ketoacyl-ACP synthases I and II, and a domain of human fatty acid synthase, have a Cys-His-His triad and also a completely conserved Lys in the active site. To examine the role of these residues in catalysis, H298Q, H298E and six K328 mutants of Escherichia colibeta-ketoacyl-ACP synthase I were constructed and their ability to carry out the trans thioesterification, decarboxylation and/or condensation steps of the reaction was ascertained. The crystal structures of wild-type and eight mutant enzymes with and/or without bound substrate were determined. The H298E enzyme shows residual decarboxylase activity in the pH range 6-8, whereas the H298Q enzyme appears to be completely decarboxylation deficient, showing that H298 serves as a catalytic base in the decarboxylation step. Lys328 has a dual role in catalysis: its charge influences acyl transfer to the active site Cys, and the steric restraint imposed on H333 is of critical importance for decarboxylation activity. This restraint makes H333 an obligate hydrogen bond donor at Nepsilon, directed only towards the active site and malonyl-ACP binding area in the fatty acid complex.

-	~~==About this Structure==~~	+	Fatty acid synthesis. Role of active site histidines and lysine in Cys-His-His-type beta-ketoacyl-acyl carrier protein synthases.,von Wettstein-Knowles P, Olsen JG, McGuire KA, Henriksen A FEBS J. 2006 Feb;273(4):695-710. PMID:16441657<ref>PMID:16441657</ref>
-	~~[[2bz3]] is a 4 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]~~. ~~Full crystallographic information is available from [http~~://~~oca~~.~~weizmann~~.ac.il/~~oca-bin/ocashort?id=2BZ3 OCA].~~	+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>

	==See Also==		==See Also==
	*[[Acyl carrier protein synthase\|Acyl carrier protein synthase]]		*[[Acyl carrier protein synthase\|Acyl carrier protein synthase]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	~~<ref group="xtra">PMID:016441657</ref>~~<references ~~group="xtra"~~/><~~references~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Bacillus coli migula 1895]]		[[Category: Bacillus coli migula 1895]]
	[[Category: Henriksen, A.]]		[[Category: Henriksen, A.]]

OCA at 14:10, 19 February 2014

OCA — Wed, 19 Feb 2014 14:10:01 GMT

←Older revision		Revision as of 14:10, 19 February 2014
Line 1:		Line 1:
-	[[Image:2bz3.png\|left\|200px]]
-
	{{STRUCTURE_2bz3\| PDB=2bz3 \| SCENE= }}		{{STRUCTURE_2bz3\| PDB=2bz3 \| SCENE= }}
-		+	===Structure of E.coli KAS I H298E mutant in complex with C12 fatty acid===
-	===~~STRUCTURE OF~~ E. ~~COLI~~ KAS I H298E ~~MUTANT IN COMPLEX WITH~~ C12 ~~FATTY ACID~~===	+
-		+
	{{ABSTRACT_PUBMED_16441657}}		{{ABSTRACT_PUBMED_16441657}}

	==About this Structure==		==About this Structure==
-	[[2bz3]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Escherichia_coli Escherichia~~ coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BZ3 OCA].	+	[[2bz3]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BZ3 OCA].

	==See Also==		==See Also==
Line 14:		Line 10:

	==Reference==		==Reference==
-	<ref group="xtra">PMID:016441657</ref><~~ref~~ group="xtra"~~>PMID:011502177<~~/~~ref~~><references ~~group="xtra"~~/>	+	<ref group="xtra">PMID:016441657</ref><references group="xtra"/><references/>
-	[[Category: ~~Beta-ketoacyl-acyl-carrier-protein synthase I]]~~	+	[[Category: Bacillus coli migula 1895]]
-	~~[[Category: Escherichia~~ coli]]	+
	[[Category: Henriksen, A.]]		[[Category: Henriksen, A.]]
	[[Category: Olsen, J G.]]		[[Category: Olsen, J G.]]
-	[[Category: Wettstein-Knowles, P ~~Von~~.]]	+	[[Category: Wettstein-Knowles, P von.]]
	[[Category: Acyltransferase]]		[[Category: Acyltransferase]]
	[[Category: Claisen condensation]]		[[Category: Claisen condensation]]

OCA at 09:52, 20 October 2012

OCA — Sat, 20 Oct 2012 09:52:43 GMT

←Older revision		Revision as of 09:52, 20 October 2012
Line 8:		Line 8:

	==About this Structure==		==About this Structure==
-	[[2bz3]] is a 4 chain structure ~~of [[Acyl carrier protein synthase]]~~ with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BZ3 OCA].	+	[[2bz3]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BZ3 OCA].

	==See Also==		==See Also==
Line 14:		Line 14:

	==Reference==		==Reference==
-	<ref group="xtra">PMID:016441657</ref><ref group="xtra">PMID:011502177~~</ref><ref group="xtra">PMID:010571059~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:016441657</ref><ref group="xtra">PMID:011502177</ref><references group="xtra"/>
	[[Category: Beta-ketoacyl-acyl-carrier-protein synthase I]]		[[Category: Beta-ketoacyl-acyl-carrier-protein synthase I]]
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]