2cvr - Revision history

OCA at 10:33, 16 August 2023

OCA — Wed, 16 Aug 2023 10:33:55 GMT

←Older revision		Revision as of 10:33, 16 August 2023
Line 1:		Line 1:

	==NMR solution structure of sso7d mutant, K12L, 12 conformers==		==NMR solution structure of sso7d mutant, K12L, 12 conformers==
-	<StructureSection load='2cvr' size='340' side='right'caption='[[2cvr~~]], [[NMR_Ensembles_of_Models \| 12 NMR models~~]]' scene=''>	+	<StructureSection load='2cvr' size='340' side='right'caption='[[2cvr]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2cvr]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Atcc_35091 Atcc 35091~~]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1r83 1r83]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CVR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2CVR FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2cvr]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharolobus_solfataricus Saccharolobus solfataricus]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1r83 1r83]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CVR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2CVR FirstGlance]. <br>
-	</td></tr><tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"~~><div style~~=~~'overflow: auto; max-height: 3em;'>[[1jic\|1jic]], [[1b4o\|1b4o]]</div~~></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2cvr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2cvr OCA], [https://pdbe.org/2cvr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2cvr RCSB], [https://www.ebi.ac.uk/pdbsum/2cvr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2cvr ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2cvr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2cvr OCA], [https://pdbe.org/2cvr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2cvr RCSB], [https://www.ebi.ac.uk/pdbsum/2cvr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2cvr ProSAT]</span></td></tr>
	</table>		</table>
-	~~<div style~~=~~"background-color:#fffaf0;">~~	+	== Function ==
-	=~~= Publication Abstract from PubMed~~ ==	+	[https://www.uniprot.org/uniprot/DN7A_SACS2 DN7A_SACS2]
-	Sso7d is a basic 7-kDa DNA-binding protein from Sulfolobus solfataricus, also endowed with ribonuclease activity. The protein consists of a double-stranded antiparallel beta-sheet, onto which an orthogonal triple-stranded antiparallel beta-sheet is packed, and of a small helical stretch at the C-terminus. Furthermore, the two beta-sheets enclose an aromatic cluster displaying a fishbone geometry. We previously cloned the Sso7d-encoding gene, expressed it in Escherichia coli, and produced several single-point mutants, either of residues located in the hydrophobic core or of Trp23, which is exposed to the solvent and plays a major role in DNA binding. The mutation F31A was dramatically destabilizing, with a loss in thermo- and piezostabilities by at least 27 K and 10 kbar, respectively. Here, we report the solution structure of the F31A mutant, which was determined by NMR spectroscopy using 744 distance constraints obtained from analysis of multidimensional spectra in conjunction with simulated annealing protocols. The most remarkable finding is the change in orientation of the Trp23 side chain, which in the wild type is completely exposed to the solvent, whereas in the mutant is largely buried in the aromatic cluster. This prevents the formation of a cavity in the hydrophobic core of the mutant, which would arise in the absence of structural rearrangements. We found additional changes produced by the mutation, notably a strong distortion in the beta-sheets with loss in several hydrogen bonds, increased flexibility of some stretches of the backbone, and some local strains. On one hand, these features may justify the dramatic destabilization provoked by the mutation; on the other hand, they highlight the crucial role of the hydrophobic core in protein stability. To the best of our knowledge, no similar rearrangement has been so far described as a result of a single-point mutation.	+
-		+
-	A single-point mutation in the extreme heat- and pressure-resistant sso7d protein from sulfolobus solfataricus leads to a major rearrangement of the hydrophobic core.,Consonni R, Santomo L, Fusi P, Tortora P, Zetta L Biochemistry. 1999 Sep 28;38(39):~~12709-17. PMID:10504241<ref>PMID:10504241<~~/~~ref>~~	+
-		+
-	~~From MEDLINE®~~/~~PubMed®, a database of the U~~.S. ~~National Library of Medicine.<br>~~	+
-	</~~div>~~	+
-	~~<div class="pdbe-citations 2cvr" style="background-color:#fffaf0;"></div>~~	+
-	~~== References ==~~	+
-	~~<references~~/>	+
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Atcc 35091]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Alberti, E]]	+	[[Category: Saccharolobus solfataricus]]
-	[[Category: Arosio, I]]	+	[[Category: Alberti E]]
-	[[Category: Consonni, R]]	+	[[Category: Arosio I]]
-	[[Category: Fusi, P]]	+	[[Category: Consonni R]]
-	[[Category: Recca, T]]	+	[[Category: Fusi P]]
-	[[Category: Zetta, L]]	+	[[Category: Recca T]]
-	~~[[Category: Dna binding protein]]~~	+	[[Category: Zetta L]]
-	~~[[Category: Dna-binding protein]]~~	+
-	~~[[Category: Single point mutation]]~~	+
-	~~[[Category: Sulfolobus solfataricus]]~~	+
-	~~[[Category: Thermostable protein~~]]	+

OCA at 06:47, 10 November 2021

OCA — Wed, 10 Nov 2021 06:47:21 GMT

←Older revision		Revision as of 06:47, 10 November 2021
Line 3:		Line 3:
	<StructureSection load='2cvr' size='340' side='right'caption='[[2cvr]], [[NMR_Ensembles_of_Models \| 12 NMR models]]' scene=''>		<StructureSection load='2cvr' size='340' side='right'caption='[[2cvr]], [[NMR_Ensembles_of_Models \| 12 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2cvr]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~'saccharolobus_solfataricus' 'saccharolobus solfataricus'~~]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1r83 1r83]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CVR OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2CVR FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2cvr]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Atcc_35091 Atcc 35091]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1r83 1r83]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CVR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2CVR FirstGlance]. <br>
-	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1jic\|1jic]], [[1b4o\|1b4o]]</td></tr>	+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1jic\|1jic]], [[1b4o\|1b4o]]</div></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2cvr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2cvr OCA], [~~http~~://pdbe.org/2cvr PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2cvr RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2cvr PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2cvr ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2cvr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2cvr OCA], [https://pdbe.org/2cvr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2cvr RCSB], [https://www.ebi.ac.uk/pdbsum/2cvr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2cvr ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 20:		Line 20:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Saccharolobus solfataricus~~]]	+	[[Category: Atcc 35091]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Alberti, E]]		[[Category: Alberti, E]]

OCA at 11:02, 26 February 2020

OCA — Wed, 26 Feb 2020 11:02:30 GMT

←Older revision		Revision as of 11:02, 26 February 2020
Line 1:		Line 1:

	==NMR solution structure of sso7d mutant, K12L, 12 conformers==		==NMR solution structure of sso7d mutant, K12L, 12 conformers==
-	<StructureSection load='2cvr' size='340' side='right' caption='[[2cvr]], [[NMR_Ensembles_of_Models \| 12 NMR models]]' scene=''>	+	<StructureSection load='2cvr' size='340' side='right'caption='[[2cvr]], [[NMR_Ensembles_of_Models \| 12 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2cvr]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/'saccharolobus_solfataricus' 'saccharolobus solfataricus']. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1r83 1r83]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CVR OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2CVR FirstGlance]. <br>		<table><tr><td colspan='2'>[[2cvr]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/'saccharolobus_solfataricus' 'saccharolobus solfataricus']. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1r83 1r83]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CVR OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2CVR FirstGlance]. <br>
Line 21:		Line 21:
	</StructureSection>		</StructureSection>
	[[Category: Saccharolobus solfataricus]]		[[Category: Saccharolobus solfataricus]]
		+	[[Category: Large Structures]]
	[[Category: Alberti, E]]		[[Category: Alberti, E]]
	[[Category: Arosio, I]]		[[Category: Arosio, I]]

OCA at 08:28, 23 May 2018

OCA — Wed, 23 May 2018 08:28:25 GMT

←Older revision		Revision as of 08:28, 23 May 2018
Line 1:		Line 1:
		+
	==NMR solution structure of sso7d mutant, K12L, 12 conformers==		==NMR solution structure of sso7d mutant, K12L, 12 conformers==
	<StructureSection load='2cvr' size='340' side='right' caption='[[2cvr]], [[NMR_Ensembles_of_Models \| 12 NMR models]]' scene=''>		<StructureSection load='2cvr' size='340' side='right' caption='[[2cvr]], [[NMR_Ensembles_of_Models \| 12 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2cvr]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Atcc_35091 Atcc 35091~~]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1r83 1r83]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CVR OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2CVR FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2cvr]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/'saccharolobus_solfataricus' 'saccharolobus solfataricus']. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1r83 1r83]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CVR OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2CVR FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1jic\|1jic]], [[1b4o\|1b4o]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1jic\|1jic]], [[1b4o\|1b4o]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2cvr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2cvr OCA], [http://pdbe.org/2cvr PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2cvr RCSB], [http://www.ebi.ac.uk/pdbsum/2cvr PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2cvr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2cvr OCA], [http://pdbe.org/2cvr PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2cvr RCSB], [http://www.ebi.ac.uk/pdbsum/2cvr PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2cvr ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 19:		Line 20:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Atcc 35091~~]]	+	[[Category: Saccharolobus solfataricus]]
	[[Category: Alberti, E]]		[[Category: Alberti, E]]
	[[Category: Arosio, I]]		[[Category: Arosio, I]]

OCA at 23:31, 10 September 2015

OCA — Thu, 10 Sep 2015 23:31:58 GMT

←Older revision		Revision as of 23:31, 10 September 2015
Line 2:		Line 2:
	<StructureSection load='2cvr' size='340' side='right' caption='[[2cvr]], [[NMR_Ensembles_of_Models \| 12 NMR models]]' scene=''>		<StructureSection load='2cvr' size='340' side='right' caption='[[2cvr]], [[NMR_Ensembles_of_Models \| 12 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2cvr]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Sulfolobus_solfataricus Sulfolobus solfataricus~~]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1r83 1r83]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CVR OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2CVR FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2cvr]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_35091 Atcc 35091]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1r83 1r83]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CVR OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2CVR FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1jic\|1jic]], [[1b4o\|1b4o]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1jic\|1jic]], [[1b4o\|1b4o]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2cvr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2cvr OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2cvr RCSB], [http://www.ebi.ac.uk/pdbsum/2cvr PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2cvr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2cvr OCA], [http://pdbe.org/2cvr PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2cvr RCSB], [http://www.ebi.ac.uk/pdbsum/2cvr PDBsum]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 14:		Line 14:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 2cvr" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Sulfolobus solfataricus~~]]	+	[[Category: Atcc 35091]]
	[[Category: Alberti, E]]		[[Category: Alberti, E]]
	[[Category: Arosio, I]]		[[Category: Arosio, I]]
Line 28:		Line 29:
	[[Category: Dna-binding protein]]		[[Category: Dna-binding protein]]
	[[Category: Single point mutation]]		[[Category: Single point mutation]]
		+	[[Category: Sulfolobus solfataricus]]
	[[Category: Thermostable protein]]		[[Category: Thermostable protein]]

OCA at 06:29, 22 December 2014

OCA — Mon, 22 Dec 2014 06:29:36 GMT

←Older revision		Revision as of 06:29, 22 December 2014
Line 1:		Line 1:
-	~~{{STRUCTURE_2cvr\| PDB=2cvr \| SCENE= }}~~	+	==NMR solution structure of sso7d mutant, K12L, 12 conformers==
-	===NMR solution structure of sso7d mutant, K12L, 12 conformers===	+	<StructureSection load='2cvr' size='340' side='right' caption='[[2cvr]], [[NMR_Ensembles_of_Models \| 12 NMR models]]' scene=''>
-	~~{{ABSTRACT_PUBMED_10504241}}~~	+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[2cvr]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Sulfolobus_solfataricus Sulfolobus solfataricus]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1r83 1r83]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CVR OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2CVR FirstGlance]. <br>
		+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1jic\|1jic]], [[1b4o\|1b4o]]</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2cvr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2cvr OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2cvr RCSB], [http://www.ebi.ac.uk/pdbsum/2cvr PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Sso7d is a basic 7-kDa DNA-binding protein from Sulfolobus solfataricus, also endowed with ribonuclease activity. The protein consists of a double-stranded antiparallel beta-sheet, onto which an orthogonal triple-stranded antiparallel beta-sheet is packed, and of a small helical stretch at the C-terminus. Furthermore, the two beta-sheets enclose an aromatic cluster displaying a fishbone geometry. We previously cloned the Sso7d-encoding gene, expressed it in Escherichia coli, and produced several single-point mutants, either of residues located in the hydrophobic core or of Trp23, which is exposed to the solvent and plays a major role in DNA binding. The mutation F31A was dramatically destabilizing, with a loss in thermo- and piezostabilities by at least 27 K and 10 kbar, respectively. Here, we report the solution structure of the F31A mutant, which was determined by NMR spectroscopy using 744 distance constraints obtained from analysis of multidimensional spectra in conjunction with simulated annealing protocols. The most remarkable finding is the change in orientation of the Trp23 side chain, which in the wild type is completely exposed to the solvent, whereas in the mutant is largely buried in the aromatic cluster. This prevents the formation of a cavity in the hydrophobic core of the mutant, which would arise in the absence of structural rearrangements. We found additional changes produced by the mutation, notably a strong distortion in the beta-sheets with loss in several hydrogen bonds, increased flexibility of some stretches of the backbone, and some local strains. On one hand, these features may justify the dramatic destabilization provoked by the mutation; on the other hand, they highlight the crucial role of the hydrophobic core in protein stability. To the best of our knowledge, no similar rearrangement has been so far described as a result of a single-point mutation.

-	~~==About this Structure==~~	+	A single-point mutation in the extreme heat- and pressure-resistant sso7d protein from sulfolobus solfataricus leads to a major rearrangement of the hydrophobic core.,Consonni R, Santomo L, Fusi P, Tortora P, Zetta L Biochemistry. 1999 Sep 28;38(39):12709-17. PMID:10504241<ref>PMID:10504241</ref>
-	~~[[2cvr]] is a 1 chain structure with sequence~~ from ~~[http://en.wikipedia.org/wiki/Sulfolobus_solfataricus Sulfolobus~~ solfataricus~~]. This structure supersedes~~ the ~~now removed PDB entry [http://oca~~.~~weizmann~~.~~ac.il/oca~~-~~bin/send-pdb?obs=1&id=1r83 1r83]~~. ~~Full experimental information is available from [http~~:/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=2CVR OCA].~~	+

-	==~~Reference~~==	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~<ref group="xtra">PMID:010504241</ref>~~<references ~~group="xtra"~~/><~~references~~/>	+	</div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
	[[Category: Sulfolobus solfataricus]]		[[Category: Sulfolobus solfataricus]]
-	[[Category: Alberti, E.]]	+	[[Category: Alberti, E]]
-	[[Category: Arosio, I.]]	+	[[Category: Arosio, I]]
-	[[Category: Consonni, R.]]	+	[[Category: Consonni, R]]
-	[[Category: Fusi, P.]]	+	[[Category: Fusi, P]]
-	[[Category: Recca, T.]]	+	[[Category: Recca, T]]
-	[[Category: Zetta, L.]]	+	[[Category: Zetta, L]]
	[[Category: Dna binding protein]]		[[Category: Dna binding protein]]
	[[Category: Dna-binding protein]]		[[Category: Dna-binding protein]]
	[[Category: Single point mutation]]		[[Category: Single point mutation]]
-	[[Category: Sulfolobus solfataricus]]
	[[Category: Thermostable protein]]		[[Category: Thermostable protein]]

OCA at 08:19, 23 April 2014

OCA — Wed, 23 Apr 2014 08:19:08 GMT

←Older revision		Revision as of 08:19, 23 April 2014
Line 1:		Line 1:
-	[[Image:2cvr.png\|left\|200px]]
-
-	<!--
-	The line below this paragraph, containing "STRUCTURE_2cvr", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_2cvr\| PDB=2cvr \| SCENE= }}		{{STRUCTURE_2cvr\| PDB=2cvr \| SCENE= }}
-
	===NMR solution structure of sso7d mutant, K12L, 12 conformers===		===NMR solution structure of sso7d mutant, K12L, 12 conformers===
-
-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_10504241}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 10504241 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_10504241}}		{{ABSTRACT_PUBMED_10504241}}

Line 22:		Line 7:

	==Reference==		==Reference==
-	<ref group="xtra">PMID:010504241</ref><~~ref~~ group="xtra"~~>PMID:012578354<~~/~~ref~~><references ~~group="xtra"~~/>	+	<ref group="xtra">PMID:010504241</ref><references group="xtra"/><references/>
	[[Category: Sulfolobus solfataricus]]		[[Category: Sulfolobus solfataricus]]
	[[Category: Alberti, E.]]		[[Category: Alberti, E.]]

OCA at 05:12, 4 April 2012

OCA — Wed, 04 Apr 2012 05:12:23 GMT

←Older revision		Revision as of 05:12, 4 April 2012
Line 19:		Line 19:

	==About this Structure==		==About this Structure==
-	[[2cvr]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Sulfolobus_solfataricus Sulfolobus solfataricus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CVR OCA].	+	[[2cvr]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Sulfolobus_solfataricus Sulfolobus solfataricus]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1r83 1r83]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CVR OCA].

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~10504241~~</ref><ref group="xtra">PMID:~~12578354~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:010504241</ref><ref group="xtra">PMID:012578354</ref><references group="xtra"/>
	[[Category: Sulfolobus solfataricus]]		[[Category: Sulfolobus solfataricus]]
	[[Category: Alberti, E.]]		[[Category: Alberti, E.]]
Line 32:		Line 32:
	[[Category: Dna binding protein]]		[[Category: Dna binding protein]]
	[[Category: Dna-binding protein]]		[[Category: Dna-binding protein]]
-	[[Category: Nmr]]
	[[Category: Single point mutation]]		[[Category: Single point mutation]]
	[[Category: Sulfolobus solfataricus]]		[[Category: Sulfolobus solfataricus]]
	[[Category: Thermostable protein]]		[[Category: Thermostable protein]]

OCA: Protected "2cvr" [edit=sysop:move=sysop]

OCA — Mon, 14 Mar 2011 20:34:30 GMT

Protected "2cvr" [edit=sysop:move=sysop]

←Older revision

Revision as of 20:34, 14 March 2011

OCA at 20:34, 14 March 2011

OCA — Mon, 14 Mar 2011 20:34:27 GMT

←Older revision		Revision as of 20:34, 14 March 2011
Line 1:		Line 1:
-	[[Image:2cvr.~~gif~~\|left\|200px]]	+	[[Image:2cvr.png\|left\|200px]]

	<!--		<!--
Line 9:		Line 9:
	{{STRUCTURE_2cvr\| PDB=2cvr \| SCENE= }}		{{STRUCTURE_2cvr\| PDB=2cvr \| SCENE= }}

-	~~'''~~NMR solution structure of sso7d mutant, K12L, 12 conformers~~'''~~	+	===NMR solution structure of sso7d mutant, K12L, 12 conformers===


-	~~==Overview==~~	+	<!--
-	~~Sso7d is a basic 7~~-~~kDa DNA~~-~~binding protein from Sulfolobus solfataricus, also endowed with ribonuclease activity.~~ The ~~protein consists of a double-stranded antiparallel beta-sheet~~, ~~onto which an orthogonal triple-stranded antiparallel beta-sheet is packed~~, ~~and of a small helical stretch at~~ the ~~C-terminus. Furthermore,~~ the ~~two beta-sheets enclose an aromatic cluster displaying a fishbone geometry. We previously cloned the Sso7d-encoding gene, expressed~~ it in Escherichia coli, and produced several single-point mutants, either of residues located in the hydrophobic core or of Trp23, which is exposed to the solvent and plays a major role in DNA binding. The mutation F31A was dramatically destabilizing, with a loss in thermo- and piezostabilities by at ~~least 27 K and 10 kbar, respectively~~. Here, we report the solution structure of the F31A mutant, which was determined by NMR spectroscopy using 744 distance constraints obtained from analysis of multidimensional spectra in conjunction with simulated annealing protocols. ~~The most remarkable finding is the change in orientation of the Trp23 side chain~~, ~~which in the wild type~~ is ~~completely exposed to~~ the ~~solvent, whereas in the mutant is largely buried in the aromatic cluster~~. This prevents the formation of a cavity in the hydrophobic core of the mutant, which would arise in the absence of structural rearrangements. We found additional changes produced by the mutation, notably a strong distortion in the beta-sheets with loss in several hydrogen bonds, increased flexibility of some stretches of the backbone, and some local strains. On one hand, these features may justify the dramatic destabilization provoked by the mutation; on the other hand, they highlight the crucial role of the hydrophobic core in protein stability. To the best of our knowledge, no similar rearrangement has been so far described as a result of a single-~~point mutation.~~	+	The line below this paragraph, {{ABSTRACT_PUBMED_10504241}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 10504241 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_10504241}}

	==About this Structure==		==About this Structure==
-	~~2CVR is a~~ [[~~Single protein~~]] structure of sequence from [http://en.wikipedia.org/wiki/Sulfolobus_solfataricus Sulfolobus solfataricus]. Full ~~crystallographic~~ information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CVR OCA].	+	[[2cvr]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Sulfolobus_solfataricus Sulfolobus solfataricus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CVR OCA].

	==Reference==		==Reference==
-	A single-point mutation in the extreme heat- and pressure-resistant sso7d protein from sulfolobus solfataricus leads to a major rearrangement of the hydrophobic core., Consonni R, Santomo L, Fusi P, Tortora P, Zetta L, Biochemistry. 1999 Sep 28;38(39):~~12709-17.~~ PMID:~~[http:~~//~~www.ncbi.nlm.nih.gov/pubmed/10504241 10504241]~~	+	<ref group="xtra">PMID:10504241</ref><ref group="xtra">PMID:12578354</ref><references group="xtra"/>
-	~~[[Category: Single protein]]~~	+
	[[Category: Sulfolobus solfataricus]]		[[Category: Sulfolobus solfataricus]]
	[[Category: Alberti, E.]]		[[Category: Alberti, E.]]
Line 28:		Line 30:
	[[Category: Recca, T.]]		[[Category: Recca, T.]]
	[[Category: Zetta, L.]]		[[Category: Zetta, L.]]
		+	[[Category: Dna binding protein]]
	[[Category: Dna-binding protein]]		[[Category: Dna-binding protein]]
	[[Category: Nmr]]		[[Category: Nmr]]
Line 33:		Line 36:
	[[Category: Sulfolobus solfataricus]]		[[Category: Sulfolobus solfataricus]]
	[[Category: Thermostable protein]]		[[Category: Thermostable protein]]
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 23:09:58 2008''