2d05 - Revision history

OCA at 11:33, 22 May 2024

OCA — Wed, 22 May 2024 11:33:02 GMT

←Older revision		Revision as of 11:33, 22 May 2024
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	<StructureSection load='2d05' size='340' side='right'caption='[[2d05]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='2d05' size='340' side='right'caption='[[2d05]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2d05]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Atcc_24 Atcc 24~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2D05 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2D05 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2d05]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Niallia_circulans Niallia circulans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2D05 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2D05 FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2Å</td></tr>
-	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"~~><div style='overflow: auto; max-height: 3em;'>[[1qgi\|1qgi]]</div></td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	~~<tr~~ id~~='activity'><td class~~="~~sblockLbl~~"><~~b>Activity:</b></td><td class~~=~~"sblockDat"><span class~~=~~'plainlinks~~'>~~[https://en.wikipedia.org/wiki/Chitosanase Chitosanase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.132 3.2.1.132]~~ </~~span~~></td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2d05 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2d05 OCA], [https://pdbe.org/2d05 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2d05 RCSB], [https://www.ebi.ac.uk/pdbsum/2d05 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2d05 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2d05 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2d05 OCA], [https://pdbe.org/2d05 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2d05 RCSB], [https://www.ebi.ac.uk/pdbsum/2d05 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2d05 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/~~CHIS_BACCI CHIS_BACCI]~~] Aids in the defense against invading fungal pathogens by degrading their cell wall chitosan.	+	[https://www.uniprot.org/uniprot/CHIS_NIACI CHIS_NIACI] Aids in the defense against invading fungal pathogens by degrading their cell wall chitosan.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 34:		Line 33:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Atcc 24]]
-	[[Category: Chitosanase]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Amano, S]]	+	[[Category: Niallia circulans]]
-	[[Category: Ando, A]]	+	[[Category: Amano S]]
-	[[Category: Fukamizo, T]]	+	[[Category: Ando A]]
-	[[Category: Katsumi, T]]	+	[[Category: Fukamizo T]]
-	[[Category: Miki, K]]	+	[[Category: Katsumi T]]
-	[[Category: Nagata, Y]]	+	[[Category: Miki K]]
-	[[Category: Saito, J]]	+	[[Category: Nagata Y]]
-	[[Category: Suzuki, M]]	+	[[Category: Saito J]]
-	[[Category: Yamaguchi, K]]	+	[[Category: Suzuki M]]
-	[[Category: Yoshikawa, T]]	+	[[Category: Yamaguchi K]]
-	~~[[Category: Chitosan degradation]]~~	+	[[Category: Yoshikawa T]]
-	~~[[Category: Hydrolase~~]]	+

OCA at 06:47, 10 November 2021

OCA — Wed, 10 Nov 2021 06:47:44 GMT

←Older revision		Revision as of 06:47, 10 November 2021
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	<StructureSection load='2d05' size='340' side='right'caption='[[2d05]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='2d05' size='340' side='right'caption='[[2d05]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2d05]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Atcc_24 Atcc 24]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2D05 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2D05 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2d05]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Atcc_24 Atcc 24]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2D05 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2D05 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1qgi\|1qgi]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1qgi\|1qgi]]</div></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Chitosanase Chitosanase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.132 3.2.1.132] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Chitosanase Chitosanase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.132 3.2.1.132] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2d05 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2d05 OCA], [~~http~~://pdbe.org/2d05 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2d05 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2d05 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2d05 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2d05 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2d05 OCA], [https://pdbe.org/2d05 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2d05 RCSB], [https://www.ebi.ac.uk/pdbsum/2d05 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2d05 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/CHIS_BACCI CHIS_BACCI]] Aids in the defense against invading fungal pathogens by degrading their cell wall chitosan.	+	[[https://www.uniprot.org/uniprot/CHIS_BACCI CHIS_BACCI]] Aids in the defense against invading fungal pathogens by degrading their cell wall chitosan.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 07:58, 4 March 2020

OCA — Wed, 04 Mar 2020 07:58:15 GMT

←Older revision		Revision as of 07:58, 4 March 2020
Line 1:		Line 1:

	==Chitosanase From Bacillus circulans mutant K218P==		==Chitosanase From Bacillus circulans mutant K218P==
-	<StructureSection load='2d05' size='340' side='right' caption='[[2d05]], [[Resolution\|resolution]] 2.00Å' scene=''>	+	<StructureSection load='2d05' size='340' side='right'caption='[[2d05]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2d05]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_24 Atcc 24]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2D05 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2D05 FirstGlance]. <br>		<table><tr><td colspan='2'>[[2d05]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_24 Atcc 24]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2D05 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2D05 FirstGlance]. <br>
Line 36:		Line 36:
	[[Category: Atcc 24]]		[[Category: Atcc 24]]
	[[Category: Chitosanase]]		[[Category: Chitosanase]]
		+	[[Category: Large Structures]]
	[[Category: Amano, S]]		[[Category: Amano, S]]
	[[Category: Ando, A]]		[[Category: Ando, A]]

OCA at 06:52, 30 May 2018

OCA — Wed, 30 May 2018 06:52:13 GMT

←Older revision		Revision as of 06:52, 30 May 2018
Line 1:		Line 1:
		+
	==Chitosanase From Bacillus circulans mutant K218P==		==Chitosanase From Bacillus circulans mutant K218P==
	<StructureSection load='2d05' size='340' side='right' caption='[[2d05]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='2d05' size='340' side='right' caption='[[2d05]], [[Resolution\|resolution]] 2.00Å' scene=''>
Line 6:		Line 7:
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1qgi\|1qgi]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1qgi\|1qgi]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Chitosanase Chitosanase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.132 3.2.1.132] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Chitosanase Chitosanase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.132 3.2.1.132] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2d05 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2d05 OCA], [http://pdbe.org/2d05 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2d05 RCSB], [http://www.ebi.ac.uk/pdbsum/2d05 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2d05 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2d05 OCA], [http://pdbe.org/2d05 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2d05 RCSB], [http://www.ebi.ac.uk/pdbsum/2d05 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2d05 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 14:		Line 15:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/d0/2d05_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/d0/2d05_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>

OCA at 08:38, 9 February 2016

OCA — Tue, 09 Feb 2016 08:38:51 GMT

←Older revision		Revision as of 08:38, 9 February 2016
Line 18:		Line 18:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2d05 ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 20:31, 10 September 2015

OCA — Thu, 10 Sep 2015 20:31:41 GMT

←Older revision		Revision as of 20:31, 10 September 2015
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	<StructureSection load='2d05' size='340' side='right' caption='[[2d05]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='2d05' size='340' side='right' caption='[[2d05]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2d05]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Bacillus_circulans Bacillus circulans~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2D05 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2D05 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2d05]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_24 Atcc 24]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2D05 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2D05 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1qgi\|1qgi]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1qgi\|1qgi]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Chitosanase Chitosanase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.132 3.2.1.132] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Chitosanase Chitosanase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.132 3.2.1.132] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2d05 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2d05 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2d05 RCSB], [http://www.ebi.ac.uk/pdbsum/2d05 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2d05 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2d05 OCA], [http://pdbe.org/2d05 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2d05 RCSB], [http://www.ebi.ac.uk/pdbsum/2d05 PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 28:		Line 28:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 2d05" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Bacillus circulans~~]]	+	[[Category: Atcc 24]]
	[[Category: Chitosanase]]		[[Category: Chitosanase]]
	[[Category: Amano, S]]		[[Category: Amano, S]]

OCA at 05:46, 25 December 2014

OCA — Thu, 25 Dec 2014 05:46:58 GMT

←Older revision		Revision as of 05:46, 25 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2d05]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_circulans Bacillus circulans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2D05 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2D05 FirstGlance]. <br>		<table><tr><td colspan='2'>[[2d05]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_circulans Bacillus circulans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2D05 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2D05 FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1qgi\|1qgi]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1qgi\|1qgi]]</td></tr>
-	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Chitosanase Chitosanase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.132 3.2.1.132] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Chitosanase Chitosanase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.132 3.2.1.132] </span></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2d05 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2d05 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2d05 RCSB], [http://www.ebi.ac.uk/pdbsum/2d05 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2d05 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2d05 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2d05 RCSB], [http://www.ebi.ac.uk/pdbsum/2d05 PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/CHIS_BACCI CHIS_BACCI]] Aids in the defense against invading fungal pathogens by degrading their cell wall chitosan.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 32:		Line 34:
	[[Category: Bacillus circulans]]		[[Category: Bacillus circulans]]
	[[Category: Chitosanase]]		[[Category: Chitosanase]]
-	[[Category: Amano, S.]]	+	[[Category: Amano, S]]
-	[[Category: Ando, A.]]	+	[[Category: Ando, A]]
-	[[Category: Fukamizo, T.]]	+	[[Category: Fukamizo, T]]
-	[[Category: Katsumi, T.]]	+	[[Category: Katsumi, T]]
-	[[Category: Miki, K.]]	+	[[Category: Miki, K]]
-	[[Category: Nagata, Y.]]	+	[[Category: Nagata, Y]]
-	[[Category: Saito, J.]]	+	[[Category: Saito, J]]
-	[[Category: Suzuki, M.]]	+	[[Category: Suzuki, M]]
-	[[Category: Yamaguchi, K.]]	+	[[Category: Yamaguchi, K]]
-	[[Category: Yoshikawa, T.]]	+	[[Category: Yoshikawa, T]]
	[[Category: Chitosan degradation]]		[[Category: Chitosan degradation]]
	[[Category: Hydrolase]]		[[Category: Hydrolase]]

OCA at 01:24, 30 September 2014

OCA — Tue, 30 Sep 2014 01:24:53 GMT

←Older revision		Revision as of 01:24, 30 September 2014
Line 1:		Line 1:
-	[[~~Image:~~2d05.~~png~~\|~~left~~\|200px]]	+	==Chitosanase From Bacillus circulans mutant K218P==
		+	<StructureSection load='2d05' size='340' side='right' caption='[[2d05]], [[Resolution\|resolution]] 2.00Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[2d05]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_circulans Bacillus circulans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2D05 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2D05 FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1qgi\|1qgi]]</td></tr>
		+	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Chitosanase Chitosanase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.132 3.2.1.132] </span></td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2d05 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2d05 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2d05 RCSB], [http://www.ebi.ac.uk/pdbsum/2d05 PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/d0/2d05_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	To identify the amino acids responsible for the substrate binding of chitosanase from Bacillus circulans MH-K1 (MH-K1 chitosanase), Tyr148 and Lys218 of the chitosanase were mutated to serine and proline, respectively, and the mutated chitosanases were characterized. The enzymatic activities of Y148S and K218P were found to be 12.5% and 0.16% of the wild type, respectively. When the (GlcN)3 binding ability to the chitosanase was evaluated by fluorescence spectroscopy and thermal unfolding experiments, the binding abilities of both mutant enzymes were markedly reduced as compared with the wild type enzyme. The affinity of the enzyme for the trisaccharide decreased by 1.0 kcal/mol of binding free energy for Y148S, and 3.7 kcal/mol for K218P. The crystal structure of K218P revealed that Pro218 forms a cis-peptide bond and that the state of the flexible loop containing the 218th residue is considerably affected by the mutation. Thus, we conclude that the flexible loop containing Lys218 plays an important role in substrate binding, and that the role of Tyr148 is less critical, but still important, due to a stacking interaction or hydrogen bond.

-	~~{{STRUCTURE_2d05\| PDB=2d05 \| SCENE= }}~~	+	Bacillus circulans MH-K1 chitosanase: amino acid residues responsible for substrate binding.,Fukamizo T, Amano S, Yamaguchi K, Yoshikawa T, Katsumi T, Saito J, Suzuki M, Miki K, Nagata Y, Ando A J Biochem. 2005 Nov;138(5):563-9. PMID:16272568<ref>PMID:16272568</ref>

-	~~===Chitosanase~~ From ~~Bacillus circulans mutant K218P===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_16272568}}~~	+	== References ==
-		+	<references/>
-	~~==About this Structure==~~	+	__TOC__
-	~~[[2d05]] is~~ a ~~1 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Bacillus_circulans Bacillus circulans]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=2D05 OCA].~~	+	</StructureSection>
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:016272568<~~/~~ref~~><~~ref group="xtra">PMID:010521473</ref><ref group="xtra">PMID:015299827</ref><references group="xtra"~~/>	+
	[[Category: Bacillus circulans]]		[[Category: Bacillus circulans]]
	[[Category: Chitosanase]]		[[Category: Chitosanase]]

OCA at 12:34, 23 January 2013

OCA — Wed, 23 Jan 2013 12:34:36 GMT

←Older revision		Revision as of 12:34, 23 January 2013
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	[[Image:2d05.png\|left\|200px]]		[[Image:2d05.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_2d05", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_2d05\| PDB=2d05 \| SCENE= }}		{{STRUCTURE_2d05\| PDB=2d05 \| SCENE= }}

	===Chitosanase From Bacillus circulans mutant K218P===		===Chitosanase From Bacillus circulans mutant K218P===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_16272568}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 16272568 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_16272568}}		{{ABSTRACT_PUBMED_16272568}}

Line 22:		Line 11:

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~16272568~~</ref><ref group="xtra">PMID:~~10521473~~</ref><ref group="xtra">PMID:~~15299827~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:016272568</ref><ref group="xtra">PMID:010521473</ref><ref group="xtra">PMID:015299827</ref><references group="xtra"/>
	[[Category: Bacillus circulans]]		[[Category: Bacillus circulans]]
	[[Category: Chitosanase]]		[[Category: Chitosanase]]

OCA: Protected "2d05" [edit=sysop:move=sysop]

OCA — Mon, 14 Mar 2011 21:06:58 GMT

Protected "2d05" [edit=sysop:move=sysop]

←Older revision

Revision as of 21:06, 14 March 2011