2drf - Revision history

OCA at 10:35, 8 December 2021

OCA — Wed, 08 Dec 2021 10:35:03 GMT

←Older revision		Revision as of 10:35, 8 December 2021
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	<StructureSection load='2drf' size='340' side='right'caption='[[2drf]]' scene=''>		<StructureSection load='2drf' size='340' side='right'caption='[[2drf]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2DRF FirstGlance]. <br>	+	<table><tr><td colspan='2'>For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2DRF FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2drf FirstGlance], [~~http~~://www.ebi.ac.uk/pdbsum/2drf PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2drf ProSAT]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2drf FirstGlance], [https://www.ebi.ac.uk/pdbsum/2drf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2drf ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 07:52, 4 March 2020

OCA — Wed, 04 Mar 2020 07:52:43 GMT

←Older revision		Revision as of 07:52, 4 March 2020
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	==1-O-SINAPOYL-B-GLUCOSE:L-MALATE SINAPOYLTRANSFERASE==		==1-O-SINAPOYL-B-GLUCOSE:L-MALATE SINAPOYLTRANSFERASE==
-	<StructureSection load='2drf' size='340' side='right' caption='[[2drf]]' scene=''>	+	<StructureSection load='2drf' size='340' side='right'caption='[[2drf]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2DRF FirstGlance]. <br>		<table><tr><td colspan='2'>For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2DRF FirstGlance]. <br>
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	</StructureSection>		</StructureSection>
	[[Category: Theoretical Model]]		[[Category: Theoretical Model]]
		+	[[Category: Large Structures]]
	[[Category: Brandt, W]]		[[Category: Brandt, W]]
	[[Category: Stehle, F]]		[[Category: Stehle, F]]

OCA at 06:43, 30 May 2018

OCA — Wed, 30 May 2018 06:43:12 GMT

←Older revision		Revision as of 06:43, 30 May 2018
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2DRF FirstGlance]. <br>		<table><tr><td colspan='2'>For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2DRF FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2drf FirstGlance], [http://www.ebi.ac.uk/pdbsum/2drf PDBsum]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2drf FirstGlance], [http://www.ebi.ac.uk/pdbsum/2drf PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2drf ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 10:31, 11 February 2016

OCA — Thu, 11 Feb 2016 10:31:41 GMT

←Older revision		Revision as of 10:31, 11 February 2016
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		+	{{Theoretical_model}}
		+
	==1-O-SINAPOYL-B-GLUCOSE:L-MALATE SINAPOYLTRANSFERASE==		==1-O-SINAPOYL-B-GLUCOSE:L-MALATE SINAPOYLTRANSFERASE==
	<StructureSection load='2drf' size='340' side='right' caption='[[2drf]]' scene=''>		<StructureSection load='2drf' size='340' side='right' caption='[[2drf]]' scene=''>
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Theoretical Model]]
	[[Category: Brandt, W]]		[[Category: Brandt, W]]
	[[Category: Stehle, F]]		[[Category: Stehle, F]]

OCA at 01:06, 10 September 2015

OCA — Thu, 10 Sep 2015 01:06:38 GMT

←Older revision		Revision as of 01:06, 10 September 2015
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	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 2drf" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 16:59, 15 January 2015

OCA — Thu, 15 Jan 2015 16:59:43 GMT

←Older revision		Revision as of 16:59, 15 January 2015
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2DRF FirstGlance]. <br>		<table><tr><td colspan='2'>For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2DRF FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2drf FirstGlance], [http://www.ebi.ac.uk/pdbsum/2drf PDBsum]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2drf FirstGlance], [http://www.ebi.ac.uk/pdbsum/2drf PDBsum]</span></td></tr>
-	<table>	+	</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==

OCA at 04:47, 29 September 2014

OCA — Mon, 29 Sep 2014 04:47:40 GMT

←Older revision		Revision as of 04:47, 29 September 2014
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-	~~{{Theoretical_model}}~~	+	==1-O-SINAPOYL-B-GLUCOSE:L-MALATE SINAPOYLTRANSFERASE==
		+	<StructureSection load='2drf' size='340' side='right' caption='[[2drf]]' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2DRF FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2drf FirstGlance], [http://www.ebi.ac.uk/pdbsum/2drf PDBsum]</span></td></tr>
		+	<table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Structures of the serine carboxypeptidase-like enzymes 1-O-sinapoyl-beta-glucose:L-malate sinapoyltransferase (SMT) and 1-O-sinapoyl-beta-glucose:choline sinapoyltransferase (SCT) were modeled to gain insight into determinants of specificity and substrate recognition. The structures reveal the alpha/beta-hydrolase fold as scaffold for the catalytic triad Ser-His-Asp. The recombinant mutants of SMT Ser173Ala and His411Ala were inactive, whereas Asp358Ala displayed residual activity of 20%. 1-O-sinapoyl-beta-glucose recognition is mediated by a network of hydrogen bonds. The glucose moiety is recognized by a hydrogen bond network including Trp71, Asn73, Glu87 and Asp172. The conserved Asp172 at the sequence position preceding the catalytic serine meets sterical requirements for the glucose moiety. The mutant Asn73Ala with a residual activity of 13% underscores the importance of the intact hydrogen bond network. Arg322 is of key importance by hydrogen bonding of 1-O-sinapoyl-beta-glucose and L-malate. By conformational change, Arg322 transfers L-malate to a position favoring its activation by His411. Accordingly, the mutant Arg322Glu showed 1% residual activity. Glu215 and Arg219 establish hydrogen bonds with the sinapoyl moiety. The backbone amide hydrogens of Gly75 and Tyr174 were shown to form the oxyanion hole, stabilizing the transition state. SCT reveals also the catalytic triad and a hydrogen bond network for 1-O-sinapoyl-beta-glucose recognition, but Glu274, Glu447, Thr445 and Cys281 are crucial for positioning of choline.

-	~~[[Image~~:~~2drf~~.~~png\|left\|200px]]~~	+	Structure determinants and substrate recognition of serine carboxypeptidase-like acyltransferases from plant secondary metabolism.,Stehle F, Brandt W, Milkowski C, Strack D FEBS Lett. 2006 Nov 27;580(27):6366-74. Epub 2006 Nov 10. PMID:17094968<ref>PMID:17094968</ref>

-	~~{{STRUCTURE_2drf\| PDB=2drf \| SCENE= }}~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	==~~=1-O-SINAPOYL-B-GLUCOSE:L-MALATE SINAPOYLTRANSFERASE===~~	+	== References ==
-		+	<references/>
-	~~{{ABSTRACT_PUBMED_17094968}}~~	+	__TOC__
-		+	</StructureSection>
-	~~==Reference~~==	+
-	<~~ref group="xtra">PMID:017094968<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Brandt, W]]		[[Category: Brandt, W]]
	[[Category: Stehle, F]]		[[Category: Stehle, F]]

OCA: Protected "2drf" [edit=sysop:move=sysop]

OCA — Sun, 21 Oct 2012 09:12:17 GMT

Protected "2drf" [edit=sysop:move=sysop]

←Older revision

Revision as of 09:12, 21 October 2012

OCA at 09:12, 21 October 2012

OCA — Sun, 21 Oct 2012 09:12:13 GMT

←Older revision		Revision as of 09:12, 21 October 2012
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	{{Theoretical_model}}		{{Theoretical_model}}
-	~~{{Seed}}~~	+
	[[Image:2drf.png\|left\|200px]]		[[Image:2drf.png\|left\|200px]]

-	<!--
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	{{STRUCTURE_2drf\| PDB=2drf \| SCENE= }}		{{STRUCTURE_2drf\| PDB=2drf \| SCENE= }}

	===1-O-SINAPOYL-B-GLUCOSE:L-MALATE SINAPOYLTRANSFERASE===		===1-O-SINAPOYL-B-GLUCOSE:L-MALATE SINAPOYLTRANSFERASE===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_17094968}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 17094968 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_17094968}}		{{ABSTRACT_PUBMED_17094968}}
-
-	==About this Structure==
-	Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2DRF OCA].

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~17094968~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:017094968</ref><references group="xtra"/>
	[[Category: Brandt, W]]		[[Category: Brandt, W]]
	[[Category: Stehle, F]]		[[Category: Stehle, F]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Apr 8 07:52:51 2010''

OCA at 04:52, 8 April 2010

OCA — Thu, 08 Apr 2010 04:52:51 GMT

←Older revision		Revision as of 04:52, 8 April 2010
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-	~~'''Theoretical Model'''~~	+	{{Theoretical_model}}
		+	{{Seed}}
		+	[[Image:2drf.png\|left\|200px]]

-	The ~~entry 2DRF~~ is ~~a Theoretical Model titled '1~~-O-~~sinapoyl-b-glucose:L-malate sinapoyltransferase'.~~	+	<!--
		+	The line below this paragraph, containing "STRUCTURE_2drf", creates the "Structure Box" on the page.
		+	You may change the PDB parameter (which sets the PDB file loaded into the applet)
		+	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
		+	or leave the SCENE parameter empty for the default display.
		+	-->
		+	{{STRUCTURE_2drf\| PDB=2drf \| SCENE= }}

-	~~[[Category~~:~~Theoretical Model]]~~	+	===1-O-SINAPOYL-B-GLUCOSE:L-MALATE SINAPOYLTRANSFERASE===


-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Wed Jul 30 09~~:56:~~18 2008~~''	+	<!--
		+	The line below this paragraph, {{ABSTRACT_PUBMED_17094968}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 17094968 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_17094968}}
		+
		+	==About this Structure==
		+	Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2DRF OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:17094968</ref><references group="xtra"/>
		+	[[Category: Brandt, W]]
		+	[[Category: Stehle, F]]
		+
		+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Apr 8 07:52:51 2010''