2ex0 - Revision history

OCA at 09:20, 14 February 2024

OCA — Wed, 14 Feb 2024 09:20:06 GMT

←Older revision		Revision as of 09:20, 14 February 2024
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2ex0]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Pasteurella_multocida Pasteurella multocida]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EX0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2EX0 FirstGlance]. <br>		<table><tr><td colspan='2'>[[2ex0]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Pasteurella_multocida Pasteurella multocida]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EX0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2EX0 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ex0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ex0 OCA], [https://pdbe.org/2ex0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ex0 RCSB], [https://www.ebi.ac.uk/pdbsum/2ex0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ex0 ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.65Å</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ex0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ex0 OCA], [https://pdbe.org/2ex0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ex0 RCSB], [https://www.ebi.ac.uk/pdbsum/2ex0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ex0 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[https://www.uniprot.org/uniprot/Q15KI8_PASMD Q15KI8_PASMD]		[https://www.uniprot.org/uniprot/Q15KI8_PASMD Q15KI8_PASMD]
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	Sialyltransferases catalyze reactions that transfer a sialic acid from CMP-sialic acid to an acceptor (a structure terminated with galactose, N-acetylgalactosamine, or sialic acid). They are key enzymes that catalyze the synthesis of sialic acid-containing oligosaccharides, polysaccharides, and glycoconjugates that play pivotal roles in many critical physiological and pathological processes. The structures of a truncated multifunctional Pasteurella multocida sialyltransferase (Delta24PmST1), in the absence and presence of CMP, have been determined by X-ray crystallography at 1.65 and 2.0 A resolutions, respectively. The Delta24PmST1 exists as a monomer in solution and in crystals. Different from the reported crystal structure of a bifunctional sialyltransferase CstII that has only one Rossmann domain, the overall structure of the Delta24PmST1 consists of two separate Rossmann nucleotide-binding domains. The Delta24PmST1 structure, thus, represents the first sialyltransferase structure that belongs to the glycosyltransferase-B (GT-B) structural group. Unlike all other known GT-B structures, however, there is no C-terminal extension that interacts with the N-terminal domain in the Delta24PmST1 structure. The CMP binding site is located in the deep cleft between the two Rossmann domains. Nevertheless, the CMP only forms interactions with residues in the C-terminal domain. The binding of CMP to the protein causes a large closure movement of the N-terminal Rossmann domain toward the C-terminal nucleotide-binding domain. Ser 143 of the N-terminal domain moves up to hydrogen-bond to Tyr 388 of the C-terminal domain. Both Ser 143 and Tyr 388 form hydrogen bonds to a water molecule, which in turn hydrogen-bonds to the terminal phosphate oxygen of CMP. These interactions may trigger the closure between the two domains. Additionally, a short helix near the active site seen in the apo structure becomes disordered upon binding to CMP. This helix may swing down upon binding to donor CMP-sialic acid to form the binding pocket for an acceptor.
-
-	Cytidine 5'-monophosphate (CMP)-induced structural changes in a multifunctional sialyltransferase from Pasteurella multocida.,Ni L, Sun M, Yu H, Chokhawala H, Chen X, Fisher AJ Biochemistry. 2006 Feb 21;45(7):2139-48. PMID:16475803<ref>PMID:16475803</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 2ex0" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
	*[[Sialyltransferase 3D structures\|Sialyltransferase 3D structures]]		*[[Sialyltransferase 3D structures\|Sialyltransferase 3D structures]]
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 20:39, 16 November 2022

OCA — Wed, 16 Nov 2022 20:39:46 GMT

←Older revision		Revision as of 20:39, 16 November 2022
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	<StructureSection load='2ex0' size='340' side='right'caption='[[2ex0]], [[Resolution\|resolution]] 1.65Å' scene=''>		<StructureSection load='2ex0' size='340' side='right'caption='[[2ex0]], [[Resolution\|resolution]] 1.65Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2ex0]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~"bacterium_multocidum"_lehmann_and_neumann_1899 "bacterium multocidum" lehmann and neumann 1899~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EX0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2EX0 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2ex0]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Pasteurella_multocida Pasteurella multocida]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EX0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2EX0 FirstGlance]. <br>
-	</td></tr>~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2ex1\|2ex1]]</div></td></tr>~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ex0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ex0 OCA], [https://pdbe.org/2ex0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ex0 RCSB], [https://www.ebi.ac.uk/pdbsum/2ex0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ex0 ProSAT]</span></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Pm0188 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=747 "Bacterium multocidum" Lehmann and Neumann 1899])</td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ex0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ex0 OCA], [https://pdbe.org/2ex0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ex0 RCSB], [https://www.ebi.ac.uk/pdbsum/2ex0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ex0 ProSAT]</span></td></tr>	+
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/Q15KI8_PASMD Q15KI8_PASMD]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Bacterium multocidum lehmann and neumann 1899]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Chen, X]]	+	[[Category: Pasteurella multocida]]
-	[[Category: Fisher~~, A J~~]]	+	[[Category: Chen X]]
-	[[Category: Ni, L]]	+	[[Category: Fisher AJ]]
-	[[Category: Sun, M]]	+	[[Category: Ni L]]
-	~~[[Category: Transferase]]~~	+	[[Category: Sun M]]
-	~~[[Category: Two rossman fold~~]]	+

OCA at 12:33, 10 February 2021

OCA — Wed, 10 Feb 2021 12:33:00 GMT

←Older revision		Revision as of 12:33, 10 February 2021
Line 1:		Line 1:

	==Crystal structure of multifunctional sialyltransferase from Pasteurella Multocida==		==Crystal structure of multifunctional sialyltransferase from Pasteurella Multocida==
-	<StructureSection load='2ex0' size='340' side='right' caption='[[2ex0]], [[Resolution\|resolution]] 1.65Å' scene=''>	+	<StructureSection load='2ex0' size='340' side='right'caption='[[2ex0]], [[Resolution\|resolution]] 1.65Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2ex0]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/"bacterium_multocidum"_lehmann_and_neumann_1899 "bacterium multocidum" lehmann and neumann 1899]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EX0 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2EX0 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2ex0]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/"bacterium_multocidum"_lehmann_and_neumann_1899 "bacterium multocidum" lehmann and neumann 1899]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EX0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2EX0 FirstGlance]. <br>
-	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2ex1\|2ex1]]</td></tr>	+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2ex1\|2ex1]]</div></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Pm0188 ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=747 "Bacterium multocidum" Lehmann and Neumann 1899])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Pm0188 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=747 "Bacterium multocidum" Lehmann and Neumann 1899])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2ex0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ex0 OCA], [~~http~~://pdbe.org/2ex0 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2ex0 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2ex0 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2ex0 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ex0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ex0 OCA], [https://pdbe.org/2ex0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ex0 RCSB], [https://www.ebi.ac.uk/pdbsum/2ex0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ex0 ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 19:		Line 19:

	==See Also==		==See Also==
-	*[[Sialyltransferase\|Sialyltransferase]]	+	*[[Sialyltransferase 3D structures\|Sialyltransferase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 25:		Line 25:
	</StructureSection>		</StructureSection>
	[[Category: Bacterium multocidum lehmann and neumann 1899]]		[[Category: Bacterium multocidum lehmann and neumann 1899]]
		+	[[Category: Large Structures]]
	[[Category: Chen, X]]		[[Category: Chen, X]]
	[[Category: Fisher, A J]]		[[Category: Fisher, A J]]

OCA at 08:34, 14 June 2018

OCA — Thu, 14 Jun 2018 08:34:51 GMT

←Older revision		Revision as of 08:34, 14 June 2018
Line 1:		Line 1:
		+
	==Crystal structure of multifunctional sialyltransferase from Pasteurella Multocida==		==Crystal structure of multifunctional sialyltransferase from Pasteurella Multocida==
	<StructureSection load='2ex0' size='340' side='right' caption='[[2ex0]], [[Resolution\|resolution]] 1.65Å' scene=''>		<StructureSection load='2ex0' size='340' side='right' caption='[[2ex0]], [[Resolution\|resolution]] 1.65Å' scene=''>
Line 5:		Line 6:
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2ex1\|2ex1]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2ex1\|2ex1]]</td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Pm0188 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=747 "Bacterium multocidum" Lehmann and Neumann 1899])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Pm0188 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=747 "Bacterium multocidum" Lehmann and Neumann 1899])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ex0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ex0 OCA], [http://pdbe.org/2ex0 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2ex0 RCSB], [http://www.ebi.ac.uk/pdbsum/2ex0 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ex0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ex0 OCA], [http://pdbe.org/2ex0 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2ex0 RCSB], [http://www.ebi.ac.uk/pdbsum/2ex0 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2ex0 ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	</div>		</div>
	<div class="pdbe-citations 2ex0" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 2ex0" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Sialyltransferase\|Sialyltransferase]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 20:36, 9 September 2015

OCA — Wed, 09 Sep 2015 20:36:26 GMT

←Older revision		Revision as of 20:36, 9 September 2015
Line 5:		Line 5:
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2ex1\|2ex1]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2ex1\|2ex1]]</td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Pm0188 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=747 "Bacterium multocidum" Lehmann and Neumann 1899])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Pm0188 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=747 "Bacterium multocidum" Lehmann and Neumann 1899])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ex0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ex0 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2ex0 RCSB], [http://www.ebi.ac.uk/pdbsum/2ex0 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ex0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ex0 OCA], [http://pdbe.org/2ex0 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2ex0 RCSB], [http://www.ebi.ac.uk/pdbsum/2ex0 PDBsum]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 15:		Line 15:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 2ex0" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 05:01, 22 December 2014

OCA — Mon, 22 Dec 2014 05:01:14 GMT

←Older revision		Revision as of 05:01, 22 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2ex0]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacterium_multocidum"_lehmann_and_neumann_1899 "bacterium multocidum" lehmann and neumann 1899]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EX0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2EX0 FirstGlance]. <br>		<table><tr><td colspan='2'>[[2ex0]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacterium_multocidum"_lehmann_and_neumann_1899 "bacterium multocidum" lehmann and neumann 1899]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EX0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2EX0 FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2ex1\|2ex1]]</td></tr>	+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2ex1\|2ex1]]</td></tr>
-	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Pm0188 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=747 "Bacterium multocidum" Lehmann and Neumann 1899])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Pm0188 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=747 "Bacterium multocidum" Lehmann and Neumann 1899])</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ex0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ex0 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2ex0 RCSB], [http://www.ebi.ac.uk/pdbsum/2ex0 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ex0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ex0 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2ex0 RCSB], [http://www.ebi.ac.uk/pdbsum/2ex0 PDBsum]</span></td></tr>
-	<table>	+	</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	</StructureSection>		</StructureSection>
	[[Category: Bacterium multocidum lehmann and neumann 1899]]		[[Category: Bacterium multocidum lehmann and neumann 1899]]
-	[[Category: Chen, X.]]	+	[[Category: Chen, X]]
-	[[Category: Fisher, A J.]]	+	[[Category: Fisher, A J]]
-	[[Category: Ni, L.]]	+	[[Category: Ni, L]]
-	[[Category: Sun, M.]]	+	[[Category: Sun, M]]
	[[Category: Transferase]]		[[Category: Transferase]]
	[[Category: Two rossman fold]]		[[Category: Two rossman fold]]

OCA at 06:50, 9 June 2014

OCA — Mon, 09 Jun 2014 06:50:54 GMT

←Older revision		Revision as of 06:50, 9 June 2014
Line 1:		Line 1:
-	[[~~Image:~~2ex0.~~png~~\|~~left~~\|~~200px~~]]	+	==Crystal structure of multifunctional sialyltransferase from Pasteurella Multocida==
		+	<StructureSection load='2ex0' size='340' side='right' caption='[[2ex0]], [[Resolution\|resolution]] 1.65Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[2ex0]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacterium_multocidum"_lehmann_and_neumann_1899 "bacterium multocidum" lehmann and neumann 1899]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EX0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2EX0 FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2ex1\|2ex1]]</td></tr>
		+	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Pm0188 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=747 "Bacterium multocidum" Lehmann and Neumann 1899])</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ex0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ex0 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2ex0 RCSB], [http://www.ebi.ac.uk/pdbsum/2ex0 PDBsum]</span></td></tr>
		+	<table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Sialyltransferases catalyze reactions that transfer a sialic acid from CMP-sialic acid to an acceptor (a structure terminated with galactose, N-acetylgalactosamine, or sialic acid). They are key enzymes that catalyze the synthesis of sialic acid-containing oligosaccharides, polysaccharides, and glycoconjugates that play pivotal roles in many critical physiological and pathological processes. The structures of a truncated multifunctional Pasteurella multocida sialyltransferase (Delta24PmST1), in the absence and presence of CMP, have been determined by X-ray crystallography at 1.65 and 2.0 A resolutions, respectively. The Delta24PmST1 exists as a monomer in solution and in crystals. Different from the reported crystal structure of a bifunctional sialyltransferase CstII that has only one Rossmann domain, the overall structure of the Delta24PmST1 consists of two separate Rossmann nucleotide-binding domains. The Delta24PmST1 structure, thus, represents the first sialyltransferase structure that belongs to the glycosyltransferase-B (GT-B) structural group. Unlike all other known GT-B structures, however, there is no C-terminal extension that interacts with the N-terminal domain in the Delta24PmST1 structure. The CMP binding site is located in the deep cleft between the two Rossmann domains. Nevertheless, the CMP only forms interactions with residues in the C-terminal domain. The binding of CMP to the protein causes a large closure movement of the N-terminal Rossmann domain toward the C-terminal nucleotide-binding domain. Ser 143 of the N-terminal domain moves up to hydrogen-bond to Tyr 388 of the C-terminal domain. Both Ser 143 and Tyr 388 form hydrogen bonds to a water molecule, which in turn hydrogen-bonds to the terminal phosphate oxygen of CMP. These interactions may trigger the closure between the two domains. Additionally, a short helix near the active site seen in the apo structure becomes disordered upon binding to CMP. This helix may swing down upon binding to donor CMP-sialic acid to form the binding pocket for an acceptor.

-	~~{{STRUCTURE_2ex0\| PDB=2ex0 \| SCENE= }}~~	+	Cytidine 5'-monophosphate (CMP)-induced structural changes in a multifunctional sialyltransferase from Pasteurella multocida.,Ni L, Sun M, Yu H, Chokhawala H, Chen X, Fisher AJ Biochemistry. 2006 Feb 21;45(7):2139-48. PMID:16475803<ref>PMID:16475803</ref>

-	~~===Crystal structure~~ of ~~multifunctional sialyltransferase from Pasteurella Multocida===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_16475803}}~~	+	== References ==
-		+	<references/>
-	~~==About this Structure==~~	+	__TOC__
-	~~[[2ex0]] is a 2 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Pasteurella_multocida Pasteurella multocida]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=2EX0 OCA].~~	+	</StructureSection>
-		+	[[Category: Bacterium multocidum lehmann and neumann 1899]]
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:016475803<~~/~~ref~~><~~references group="xtra"~~/>	+
-	[[Category: ~~Pasteurella multocida~~]]	+
	[[Category: Chen, X.]]		[[Category: Chen, X.]]
	[[Category: Fisher, A J.]]		[[Category: Fisher, A J.]]

OCA: Protected "2ex0" [edit=sysop:move=sysop]

OCA — Tue, 14 Aug 2012 14:58:57 GMT

Protected "2ex0" [edit=sysop:move=sysop]

←Older revision

Revision as of 14:58, 14 August 2012

OCA at 14:58, 14 August 2012

OCA — Tue, 14 Aug 2012 14:58:55 GMT

←Older revision		Revision as of 14:58, 14 August 2012
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-	{{Seed}}
	[[Image:2ex0.png\|left\|200px]]		[[Image:2ex0.png\|left\|200px]]

-	<!--
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	{{STRUCTURE_2ex0\| PDB=2ex0 \| SCENE= }}		{{STRUCTURE_2ex0\| PDB=2ex0 \| SCENE= }}

	===Crystal structure of multifunctional sialyltransferase from Pasteurella Multocida===		===Crystal structure of multifunctional sialyltransferase from Pasteurella Multocida===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_16475803}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 16475803 is the PubMed ID number.
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	{{ABSTRACT_PUBMED_16475803}}		{{ABSTRACT_PUBMED_16475803}}

	==About this Structure==		==About this Structure==
-	Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EX0 OCA].	+	[[2ex0]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Pasteurella_multocida Pasteurella multocida]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EX0 OCA].

	==Reference==		==Reference==
-	Cytidine 5'-monophosphate (CMP)-induced structural changes in a multifunctional sialyltransferase from Pasteurella multocida., Ni L, Sun M, Yu H, Chokhawala H, Chen X, Fisher AJ, Biochemistry. 2006 Feb 21;45(7):2139-48. PMID:[~~http~~:~~//www.ncbi.nlm.nih.gov/pubmed/16475803 16475803~~]	+	<ref group="xtra">PMID:016475803</ref><references group="xtra"/>
		+	[[Category: Pasteurella multocida]]
	[[Category: Chen, X.]]		[[Category: Chen, X.]]
	[[Category: Fisher, A J.]]		[[Category: Fisher, A J.]]
	[[Category: Ni, L.]]		[[Category: Ni, L.]]
	[[Category: Sun, M.]]		[[Category: Sun, M.]]
		+	[[Category: Transferase]]
	[[Category: Two rossman fold]]		[[Category: Two rossman fold]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 12:25:40 2008''

OCA at 09:25, 27 July 2008

OCA — Sun, 27 Jul 2008 09:25:49 GMT

←Older revision		Revision as of 09:25, 27 July 2008
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-	[[Image:2ex0.~~gif~~\|left\|200px]]	+	{{Seed}}
		+	[[Image:2ex0.png\|left\|200px]]

	<!--		<!--
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	{{STRUCTURE_2ex0\| PDB=2ex0 \| SCENE= }}		{{STRUCTURE_2ex0\| PDB=2ex0 \| SCENE= }}

-	~~'''~~Crystal structure of multifunctional sialyltransferase from Pasteurella Multocida~~'''~~	+	===Crystal structure of multifunctional sialyltransferase from Pasteurella Multocida===


-	~~==Overview==~~	+	<!--
-	~~Sialyltransferases catalyze reactions that transfer a sialic acid from CMP~~-~~sialic acid to an acceptor (a structure terminated with galactose, N~~-acetylgalactosamine, or sialic acid). They are key enzymes that catalyze the synthesis of sialic acid-containing oligosaccharides, polysaccharides, and glycoconjugates that play pivotal roles in many critical physiological and pathological processes. The ~~structures of a truncated multifunctional Pasteurella multocida sialyltransferase (Delta24PmST1)~~, ~~in the absence and presence of CMP~~, have been determined by X-ray crystallography at 1.65 and 2.0 A resolutions, respectively. The Delta24PmST1 exists as a monomer in solution and in crystals. Different from the reported crystal structure of a bifunctional sialyltransferase CstII that has only one Rossmann domain, the overall structure of the Delta24PmST1 consists of two separate Rossmann nucleotide-binding domains. The Delta24PmST1 structure, thus, represents the ~~first sialyltransferase structure that belongs~~ to the ~~glycosyltransferase-B~~ (~~GT-B~~) ~~structural group. Unlike all other known GT-B structures, however~~, ~~there~~ is ~~no C-terminal extension that interacts with~~ the ~~N-terminal domain in the Delta24PmST1 structure~~. ~~The CMP binding site is located in the deep cleft between the two Rossmann domains. Nevertheless, the CMP only forms interactions with residues in the C~~-~~terminal domain. The binding of CMP to the protein causes a large closure movement of the N~~-terminal Rossmann domain toward the C-terminal nucleotide-binding domain. Ser 143 of the N-terminal domain moves up to hydrogen-bond to Tyr 388 of the C-terminal domain. Both Ser 143 and Tyr 388 form hydrogen bonds to a water molecule, which in turn hydrogen-bonds to the terminal phosphate oxygen of CMP. These interactions may trigger the closure between the two domains. Additionally, a short helix near the active site seen in the apo structure becomes disordered upon binding to CMP. This helix may swing down upon binding to donor CMP-sialic acid to form the binding pocket for an acceptor.	+	The line below this paragraph, {{ABSTRACT_PUBMED_16475803}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 16475803 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_16475803}}

	==About this Structure==		==About this Structure==
Line 25:		Line 29:
	[[Category: Sun, M.]]		[[Category: Sun, M.]]
	[[Category: Two rossman fold]]		[[Category: Two rossman fold]]
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun ~~May 4 03:~~12:36 2008''	+
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