2f5w - Revision history

OCA at 07:43, 23 August 2023

OCA — Wed, 23 Aug 2023 07:43:35 GMT

←Older revision		Revision as of 07:43, 23 August 2023
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	<StructureSection load='2f5w' size='340' side='right'caption='[[2f5w]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='2f5w' size='340' side='right'caption='[[2f5w]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2f5w]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/~~"bacillus_amyloliquifaciens"_(sic)_fukumoto_1943 "bacillus amyloliquifaciens" (sic) fukumoto 1943~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2F5W OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2F5W FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2f5w]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_amyloliquefaciens Bacillus amyloliquefaciens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2F5W OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2F5W FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=TOU:THIOUREA'>TOU</scene>~~, ~~<scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.001Å</td></tr>
-	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"><~~div style~~='~~overflow~~: ~~auto; max-height: 3em;~~'>~~[[2f4y\|2f4y]]~~, ~~[[2f56\|2f56]], [[2f5m\|2f5m]]~~</~~div~~></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=TOU:THIOUREA'>TOU</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2f5w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2f5w OCA], [https://pdbe.org/2f5w PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2f5w RCSB], [https://www.ebi.ac.uk/pdbsum/2f5w PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2f5w ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2f5w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2f5w OCA], [https://pdbe.org/2f5w PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2f5w RCSB], [https://www.ebi.ac.uk/pdbsum/2f5w PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2f5w ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/RNBR_BACAM RNBR_BACAM]] Hydrolyzes phosphodiester bonds in RNA, poly- and oligoribonucleotides resulting in 3'-nucleoside monophosphates via 2',3'-cyclophosphate intermediates.	+	[https://www.uniprot.org/uniprot/RNBR_BACAM RNBR_BACAM] Hydrolyzes phosphodiester bonds in RNA, poly- and oligoribonucleotides resulting in 3'-nucleoside monophosphates via 2',3'-cyclophosphate intermediates.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Bacillus amyloliquefaciens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Prange, T]]	+	[[Category: Prange T]]
-	[[Category: Salem, M]]	+	[[Category: Salem M]]
-	~~[[Category: Barnase]]~~	+
-	~~[[Category: Bromoethanol]]~~	+
-	~~[[Category: Cross-linked crystal]]~~	+
-	~~[[Category: Denaturation]]~~	+
-	~~[[Category: Glutaraldehyde]]~~	+
-	~~[[Category: Hydrolase]]~~	+
-	~~[[Category: Lysozyme]]~~	+
-	~~[[Category: Thiourea]]~~	+
-	~~[[Category: Urea~~]]	+

OCA at 09:53, 5 May 2021

OCA — Wed, 05 May 2021 09:53:23 GMT

←Older revision		Revision as of 09:53, 5 May 2021
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	<StructureSection load='2f5w' size='340' side='right'caption='[[2f5w]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='2f5w' size='340' side='right'caption='[[2f5w]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2f5w]] is a 3 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/"bacillus_amyloliquifaciens"_(sic)_fukumoto_1943 "bacillus amyloliquifaciens" (sic) fukumoto 1943]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2F5W OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2F5W FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2f5w]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/"bacillus_amyloliquifaciens"_(sic)_fukumoto_1943 "bacillus amyloliquifaciens" (sic) fukumoto 1943]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2F5W OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2F5W FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=TOU:THIOUREA'>TOU</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=TOU:THIOUREA'>TOU</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2f4y\|2f4y]], [[2f56\|2f56]], [[2f5m\|2f5m]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2f4y\|2f4y]], [[2f56\|2f56]], [[2f5m\|2f5m]]</div></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2f5w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2f5w OCA], [~~http~~://pdbe.org/2f5w PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2f5w RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2f5w PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2f5w ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2f5w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2f5w OCA], [https://pdbe.org/2f5w PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2f5w RCSB], [https://www.ebi.ac.uk/pdbsum/2f5w PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2f5w ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/RNBR_BACAM RNBR_BACAM]] Hydrolyzes phosphodiester bonds in RNA, poly- and oligoribonucleotides resulting in 3'-nucleoside monophosphates via 2',3'-cyclophosphate intermediates.	+	[[https://www.uniprot.org/uniprot/RNBR_BACAM RNBR_BACAM]] Hydrolyzes phosphodiester bonds in RNA, poly- and oligoribonucleotides resulting in 3'-nucleoside monophosphates via 2',3'-cyclophosphate intermediates.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	==See Also==		==See Also==
	*[[Barnase 3D structures\|Barnase 3D structures]]		*[[Barnase 3D structures\|Barnase 3D structures]]
-	*[[Ribonuclease\|Ribonuclease]]	+	*[[Ribonuclease 3D structures\|Ribonuclease 3D structures]]
-	*[[Temp\|Temp]]	+
	== References ==		== References ==
	<references/>		<references/>

OCA at 09:33, 24 July 2019

OCA — Wed, 24 Jul 2019 09:33:39 GMT

←Older revision		Revision as of 09:33, 24 July 2019
Line 1:		Line 1:

	==Cross-linked barnase soaked in 3 M thiourea==		==Cross-linked barnase soaked in 3 M thiourea==
-	<StructureSection load='2f5w' size='340' side='right' caption='[[2f5w]], [[Resolution\|resolution]] 2.00Å' scene=''>	+	<StructureSection load='2f5w' size='340' side='right'caption='[[2f5w]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2f5w]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_amyloliquifaciens"_(sic)_fukumoto_1943 "bacillus amyloliquifaciens" (sic) fukumoto 1943]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2F5W OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2F5W FirstGlance]. <br>		<table><tr><td colspan='2'>[[2f5w]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_amyloliquifaciens"_(sic)_fukumoto_1943 "bacillus amyloliquifaciens" (sic) fukumoto 1943]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2F5W OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2F5W FirstGlance]. <br>
Line 14:		Line 14:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/f5/2f5w_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/f5/2f5w_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
Line 31:		Line 31:

	==See Also==		==See Also==
		+	*[[Barnase 3D structures\|Barnase 3D structures]]
	*[[Ribonuclease\|Ribonuclease]]		*[[Ribonuclease\|Ribonuclease]]
	*[[Temp\|Temp]]		*[[Temp\|Temp]]
Line 37:		Line 38:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Prange, T]]		[[Category: Prange, T]]
	[[Category: Salem, M]]		[[Category: Salem, M]]

OCA at 07:34, 18 October 2017

OCA — Wed, 18 Oct 2017 07:34:00 GMT

←Older revision		Revision as of 07:34, 18 October 2017
Line 1:		Line 1:
		+
	==Cross-linked barnase soaked in 3 M thiourea==		==Cross-linked barnase soaked in 3 M thiourea==
	<StructureSection load='2f5w' size='340' side='right' caption='[[2f5w]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='2f5w' size='340' side='right' caption='[[2f5w]], [[Resolution\|resolution]] 2.00Å' scene=''>
Line 5:		Line 6:
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=TOU:THIOUREA'>TOU</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=TOU:THIOUREA'>TOU</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2f4y\|2f4y]], [[2f56\|2f56]], [[2f5m\|2f5m]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2f4y\|2f4y]], [[2f56\|2f56]], [[2f5m\|2f5m]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2f5w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2f5w OCA], [http://pdbe.org/2f5w PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2f5w RCSB], [http://www.ebi.ac.uk/pdbsum/2f5w PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2f5w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2f5w OCA], [http://pdbe.org/2f5w PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2f5w RCSB], [http://www.ebi.ac.uk/pdbsum/2f5w PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2f5w ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
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	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2f5w ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	==See Also==		==See Also==
-	*[[Barnase\|Barnase]]
	*[[Ribonuclease\|Ribonuclease]]		*[[Ribonuclease\|Ribonuclease]]
	*[[Temp\|Temp]]		*[[Temp\|Temp]]

OCA at 17:08, 11 September 2015

OCA — Fri, 11 Sep 2015 17:08:38 GMT

←Older revision		Revision as of 17:08, 11 September 2015
Line 2:		Line 2:
	<StructureSection load='2f5w' size='340' side='right' caption='[[2f5w]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='2f5w' size='340' side='right' caption='[[2f5w]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2f5w]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Bacillus_amyloliquefaciens Bacillus amyloliquefaciens~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2F5W OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2F5W FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2f5w]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_amyloliquifaciens"_(sic)_fukumoto_1943 "bacillus amyloliquifaciens" (sic) fukumoto 1943]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2F5W OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2F5W FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=TOU:THIOUREA'>TOU</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=TOU:THIOUREA'>TOU</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2f4y\|2f4y]], [[2f56\|2f56]], [[2f5m\|2f5m]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2f4y\|2f4y]], [[2f56\|2f56]], [[2f5m\|2f5m]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2f5w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2f5w OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2f5w RCSB], [http://www.ebi.ac.uk/pdbsum/2f5w PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2f5w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2f5w OCA], [http://pdbe.org/2f5w PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2f5w RCSB], [http://www.ebi.ac.uk/pdbsum/2f5w PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 27:		Line 27:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 2f5w" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 32:		Line 33:
	*[[Ribonuclease\|Ribonuclease]]		*[[Ribonuclease\|Ribonuclease]]
	*[[Temp\|Temp]]		*[[Temp\|Temp]]
-	*[[User:Jaime.Prilusky/Test/tree\|User:Jaime.Prilusky/Test/tree]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Bacillus amyloliquefaciens]]
	[[Category: Prange, T]]		[[Category: Prange, T]]
	[[Category: Salem, M]]		[[Category: Salem, M]]

OCA at 19:24, 25 December 2014

OCA — Thu, 25 Dec 2014 19:24:26 GMT

←Older revision		Revision as of 19:24, 25 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2f5w]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_amyloliquefaciens Bacillus amyloliquefaciens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2F5W OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2F5W FirstGlance]. <br>		<table><tr><td colspan='2'>[[2f5w]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_amyloliquefaciens Bacillus amyloliquefaciens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2F5W OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2F5W FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=TOU:THIOUREA'>TOU</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=TOU:THIOUREA'>TOU</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2f4y\|2f4y]], [[2f56\|2f56]], [[2f5m\|2f5m]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2f4y\|2f4y]], [[2f56\|2f56]], [[2f5m\|2f5m]]</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2f5w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2f5w OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2f5w RCSB], [http://www.ebi.ac.uk/pdbsum/2f5w PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2f5w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2f5w OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2f5w RCSB], [http://www.ebi.ac.uk/pdbsum/2f5w PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/RNBR_BACAM RNBR_BACAM]] Hydrolyzes phosphodiester bonds in RNA, poly- and oligoribonucleotides resulting in 3'-nucleoside monophosphates via 2',3'-cyclophosphate intermediates.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 29:		Line 31:
	*[[Barnase\|Barnase]]		*[[Barnase\|Barnase]]
	*[[Ribonuclease\|Ribonuclease]]		*[[Ribonuclease\|Ribonuclease]]
		+	*[[Temp\|Temp]]
		+	*[[User:Jaime.Prilusky/Test/tree\|User:Jaime.Prilusky/Test/tree]]
	== References ==		== References ==
	<references/>		<references/>
Line 34:		Line 38:
	</StructureSection>		</StructureSection>
	[[Category: Bacillus amyloliquefaciens]]		[[Category: Bacillus amyloliquefaciens]]
-	[[Category: Prange, T.]]	+	[[Category: Prange, T]]
-	[[Category: Salem, M.]]	+	[[Category: Salem, M]]
	[[Category: Barnase]]		[[Category: Barnase]]
	[[Category: Bromoethanol]]		[[Category: Bromoethanol]]

OCA at 02:38, 29 September 2014

OCA — Mon, 29 Sep 2014 02:38:23 GMT

←Older revision		Revision as of 02:38, 29 September 2014
Line 1:		Line 1:
-	[[~~Image:~~2f5w.~~png~~\|~~left~~\|200px]]	+	==Cross-linked barnase soaked in 3 M thiourea==
		+	<StructureSection load='2f5w' size='340' side='right' caption='[[2f5w]], [[Resolution\|resolution]] 2.00Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[2f5w]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_amyloliquefaciens Bacillus amyloliquefaciens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2F5W OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2F5W FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=TOU:THIOUREA'>TOU</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2f4y\|2f4y]], [[2f56\|2f56]], [[2f5m\|2f5m]]</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2f5w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2f5w OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2f5w RCSB], [http://www.ebi.ac.uk/pdbsum/2f5w PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/f5/2f5w_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Structural data about the early step of protein denaturation were obtained from cross-linked crystals for two small proteins: barnase and lysozyme. Several denaturant agents like urea, bromoethanol or thiourea were used at increasing concentrations up to a limit leading to crystal disruption (>or=2 to 6 M). Before the complete destruction of the crystal order started, specific binding sites were observed at the protein surfaces, an indication that the preliminary step of denaturation is the disproportion of intermolecular polar bonds to the benefit of the agent "parasiting" the surface. The analysis of the thermal factors first agree with a stabilization effect at low or moderate concentration of denaturants rapidly followed by a destabilization at specific weak points when the number of sites increase (overflooding effect).

-	~~{{STRUCTURE_2f5w\| PDB=2f5w \| SCENE= }}~~	+	On the edge of the denaturation process: application of X-ray diffraction to barnase and lysozyme cross-linked crystals with denaturants in molar concentrations.,Salem M, Mauguen Y, Prange T Biochim Biophys Acta. 2006 May;1764(5):903-12. Epub 2006 Mar 20. PMID:16600702<ref>PMID:16600702</ref>

-	~~===Cross-linked barnase soaked in 3 M thiourea===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_16600702}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[2f5w]] is~~ a ~~3 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Bacillus_amyloliquefaciens Bacillus amyloliquefaciens]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=2F5W OCA].~~	+

	==See Also==		==See Also==
	*[[Barnase\|Barnase]]		*[[Barnase\|Barnase]]
	*[[Ribonuclease\|Ribonuclease]]		*[[Ribonuclease\|Ribonuclease]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	<~~ref group="xtra">PMID:016600702<~~/~~ref~~><~~references group="xtra"~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Bacillus amyloliquefaciens]]		[[Category: Bacillus amyloliquefaciens]]
	[[Category: Prange, T.]]		[[Category: Prange, T.]]

OCA at 20:30, 20 October 2012

OCA — Sat, 20 Oct 2012 20:30:54 GMT

←Older revision		Revision as of 20:30, 20 October 2012
Line 8:		Line 8:

	==About this Structure==		==About this Structure==
-	[[2f5w]] is a 3 chain structure ~~of [[Barnase]] and [[Ribonuclease]]~~ with sequence from [http://en.wikipedia.org/wiki/Bacillus_amyloliquefaciens Bacillus amyloliquefaciens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2F5W OCA].	+	[[2f5w]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_amyloliquefaciens Bacillus amyloliquefaciens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2F5W OCA].

	==See Also==		==See Also==

OCA at 10:44, 27 July 2012

OCA — Fri, 27 Jul 2012 10:44:19 GMT

←Older revision		Revision as of 10:44, 27 July 2012
Line 1:		Line 1:
	[[Image:2f5w.png\|left\|200px]]		[[Image:2f5w.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_2f5w", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_2f5w\| PDB=2f5w \| SCENE= }}		{{STRUCTURE_2f5w\| PDB=2f5w \| SCENE= }}

	===Cross-linked barnase soaked in 3 M thiourea===		===Cross-linked barnase soaked in 3 M thiourea===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_16600702}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 16600702 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_16600702}}		{{ABSTRACT_PUBMED_16600702}}

Line 22:		Line 11:

	==See Also==		==See Also==
-	*[[Barnase]]	+	*[[Barnase\|Barnase]]
-	*[[Ribonuclease]]	+	*[[Ribonuclease\|Ribonuclease]]

	==Reference==		==Reference==

OCA: Protected "2f5w" [edit=sysop:move=sysop]

OCA — Thu, 08 Mar 2012 04:56:35 GMT

Protected "2f5w" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:56, 8 March 2012