2fbx - Revision history

OCA at 09:24, 30 August 2023

OCA — Wed, 30 Aug 2023 09:24:42 GMT

←Older revision		Revision as of 09:24, 30 August 2023
Line 3:		Line 3:
	<StructureSection load='2fbx' size='340' side='right'caption='[[2fbx]], [[Resolution\|resolution]] 2.20Å' scene=''>		<StructureSection load='2fbx' size='340' side='right'caption='[[2fbx]], [[Resolution\|resolution]] 2.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2fbx]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Human Human~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FBX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2FBX FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2fbx]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FBX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2FBX FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.2Å</td></tr>
-	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"~~><div style~~=~~'overflow: auto; max-height: 3em;'~~>~~[[2fbt\|2fbt]], [[2fbv\|2fbv]], [[2fby\|2fby]], [[2fc0\|2fc0]]~~<~~/div></td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
-	~~<tr id~~='~~gene'><td class~~=~~"sblockLbl"><b>[[Gene\|Gene~~:~~]]</b~~></td>~~<td class="sblockDat">WRN, RECQ3, RECQL2 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])~~</td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2fbx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2fbx OCA], [https://pdbe.org/2fbx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2fbx RCSB], [https://www.ebi.ac.uk/pdbsum/2fbx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2fbx ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2fbx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2fbx OCA], [https://pdbe.org/2fbx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2fbx RCSB], [https://www.ebi.ac.uk/pdbsum/2fbx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2fbx ProSAT]</span></td></tr>
	</table>		</table>
	== Disease ==		== Disease ==
-	[[https://www.uniprot.org/uniprot/WRN_HUMAN WRN_HUMAN]] Defects in WRN are a cause of Werner syndrome (WRN) [MIM:[https://omim.org/entry/277700 277700]]. WRN is a rare autosomal recessive progeroid syndrome characterized by the premature onset of multiple age-related disorders, including atherosclerosis, cancer, non-insulin-dependent diabetes mellitus, ocular cataracts and osteoporosis. The major cause of death, at a median age of 47, is myocardial infarction. Currently all known WS mutations produces prematurely terminated proteins.<ref>PMID:16673358</ref> Defects in WRN may be a cause of colorectal cancer (CRC) [MIM:[https://omim.org/entry/114500 114500]].	+	[https://www.uniprot.org/uniprot/WRN_HUMAN WRN_HUMAN] Defects in WRN are a cause of Werner syndrome (WRN) [MIM:[https://omim.org/entry/277700 277700]. WRN is a rare autosomal recessive progeroid syndrome characterized by the premature onset of multiple age-related disorders, including atherosclerosis, cancer, non-insulin-dependent diabetes mellitus, ocular cataracts and osteoporosis. The major cause of death, at a median age of 47, is myocardial infarction. Currently all known WS mutations produces prematurely terminated proteins.<ref>PMID:16673358</ref> Defects in WRN may be a cause of colorectal cancer (CRC) [MIM:[https://omim.org/entry/114500 114500].
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/WRN_HUMAN WRN_HUMAN]] Multifunctional enzyme that has both magnesium and ATP-dependent DNA-helicase activity and 3'->5' exonuclease activity towards double-stranded DNA with a 5'-overhang. Has no nuclease activity towards single-stranded DNA or blunt-ended double-stranded DNA. Binds preferentially to DNA substrates containing alternate secondary structures, such as replication forks and Holliday junctions. May play an important role in the dissociation of joint DNA molecules that can arise as products of homologous recombination, at stalled replication forks or during DNA repair. Alleviates stalling of DNA polymerases at the site of DNA lesions. Important for genomic integrity. Plays a role in the formation of DNA replication focal centers; stably associates with foci elements generating binding sites for RP-A (By similarity).<ref>PMID:11863428</ref> <ref>PMID:17563354</ref> <ref>PMID:18596042</ref> <ref>PMID:19652551</ref> <ref>PMID:19283071</ref>	+	[https://www.uniprot.org/uniprot/WRN_HUMAN WRN_HUMAN] Multifunctional enzyme that has both magnesium and ATP-dependent DNA-helicase activity and 3'->5' exonuclease activity towards double-stranded DNA with a 5'-overhang. Has no nuclease activity towards single-stranded DNA or blunt-ended double-stranded DNA. Binds preferentially to DNA substrates containing alternate secondary structures, such as replication forks and Holliday junctions. May play an important role in the dissociation of joint DNA molecules that can arise as products of homologous recombination, at stalled replication forks or during DNA repair. Alleviates stalling of DNA polymerases at the site of DNA lesions. Important for genomic integrity. Plays a role in the formation of DNA replication focal centers; stably associates with foci elements generating binding sites for RP-A (By similarity).<ref>PMID:11863428</ref> <ref>PMID:17563354</ref> <ref>PMID:18596042</ref> <ref>PMID:19652551</ref> <ref>PMID:19283071</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Human~~]]	+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Perry~~, J J]]~~	+	[[Category: Perry JJ]]
-	~~[[Category: 3'-5' exonuclease]]~~	+
-	~~[[Category: Dnaq family]]~~	+
-	~~[[Category: Recq]]~~	+
-	~~[[Category: Transferase]]~~	+
-	~~[[Category: Werner syndrome]]~~	+
-	~~[[Category: Wrn~~]]	+

OCA at 07:14, 24 February 2021

OCA — Wed, 24 Feb 2021 07:14:33 GMT

←Older revision		Revision as of 07:14, 24 February 2021
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	==WRN exonuclease, Mg complex==		==WRN exonuclease, Mg complex==
-	<StructureSection load='2fbx' size='340' side='right' caption='[[2fbx]], [[Resolution\|resolution]] 2.20Å' scene=''>	+	<StructureSection load='2fbx' size='340' side='right'caption='[[2fbx]], [[Resolution\|resolution]] 2.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2fbx]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FBX OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2FBX FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2fbx]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FBX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2FBX FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2fbt\|2fbt]], [[2fbv\|2fbv]], [[2fby\|2fby]], [[2fc0\|2fc0]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2fbt\|2fbt]], [[2fbv\|2fbv]], [[2fby\|2fby]], [[2fc0\|2fc0]]</div></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">WRN, RECQ3, RECQL2 ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">WRN, RECQ3, RECQL2 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2fbx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2fbx OCA], [~~http~~://pdbe.org/2fbx PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2fbx RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2fbx PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2fbx ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2fbx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2fbx OCA], [https://pdbe.org/2fbx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2fbx RCSB], [https://www.ebi.ac.uk/pdbsum/2fbx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2fbx ProSAT]</span></td></tr>
	</table>		</table>
	== Disease ==		== Disease ==
-	[[~~http~~://www.uniprot.org/uniprot/WRN_HUMAN WRN_HUMAN]] Defects in WRN are a cause of Werner syndrome (WRN) [MIM:[~~http~~://omim.org/entry/277700 277700]]. WRN is a rare autosomal recessive progeroid syndrome characterized by the premature onset of multiple age-related disorders, including atherosclerosis, cancer, non-insulin-dependent diabetes mellitus, ocular cataracts and osteoporosis. The major cause of death, at a median age of 47, is myocardial infarction. Currently all known WS mutations produces prematurely terminated proteins.<ref>PMID:16673358</ref> Defects in WRN may be a cause of colorectal cancer (CRC) [MIM:[~~http~~://omim.org/entry/114500 114500]].	+	[[https://www.uniprot.org/uniprot/WRN_HUMAN WRN_HUMAN]] Defects in WRN are a cause of Werner syndrome (WRN) [MIM:[https://omim.org/entry/277700 277700]]. WRN is a rare autosomal recessive progeroid syndrome characterized by the premature onset of multiple age-related disorders, including atherosclerosis, cancer, non-insulin-dependent diabetes mellitus, ocular cataracts and osteoporosis. The major cause of death, at a median age of 47, is myocardial infarction. Currently all known WS mutations produces prematurely terminated proteins.<ref>PMID:16673358</ref> Defects in WRN may be a cause of colorectal cancer (CRC) [MIM:[https://omim.org/entry/114500 114500]].
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/WRN_HUMAN WRN_HUMAN]] Multifunctional enzyme that has both magnesium and ATP-dependent DNA-helicase activity and 3'->5' exonuclease activity towards double-stranded DNA with a 5'-overhang. Has no nuclease activity towards single-stranded DNA or blunt-ended double-stranded DNA. Binds preferentially to DNA substrates containing alternate secondary structures, such as replication forks and Holliday junctions. May play an important role in the dissociation of joint DNA molecules that can arise as products of homologous recombination, at stalled replication forks or during DNA repair. Alleviates stalling of DNA polymerases at the site of DNA lesions. Important for genomic integrity. Plays a role in the formation of DNA replication focal centers; stably associates with foci elements generating binding sites for RP-A (By similarity).<ref>PMID:11863428</ref> <ref>PMID:17563354</ref> <ref>PMID:18596042</ref> <ref>PMID:19652551</ref> <ref>PMID:19283071</ref>	+	[[https://www.uniprot.org/uniprot/WRN_HUMAN WRN_HUMAN]] Multifunctional enzyme that has both magnesium and ATP-dependent DNA-helicase activity and 3'->5' exonuclease activity towards double-stranded DNA with a 5'-overhang. Has no nuclease activity towards single-stranded DNA or blunt-ended double-stranded DNA. Binds preferentially to DNA substrates containing alternate secondary structures, such as replication forks and Holliday junctions. May play an important role in the dissociation of joint DNA molecules that can arise as products of homologous recombination, at stalled replication forks or during DNA repair. Alleviates stalling of DNA polymerases at the site of DNA lesions. Important for genomic integrity. Plays a role in the formation of DNA replication focal centers; stably associates with foci elements generating binding sites for RP-A (By similarity).<ref>PMID:11863428</ref> <ref>PMID:17563354</ref> <ref>PMID:18596042</ref> <ref>PMID:19652551</ref> <ref>PMID:19283071</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fb/2fbx_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fb/2fbx_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
Line 32:		Line 32:
	</div>		</div>
	<div class="pdbe-citations 2fbx" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 2fbx" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Helicase 3D structures\|Helicase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 37:		Line 40:
	</StructureSection>		</StructureSection>
	[[Category: Human]]		[[Category: Human]]
		+	[[Category: Large Structures]]
	[[Category: Perry, J J]]		[[Category: Perry, J J]]
	[[Category: 3'-5' exonuclease]]		[[Category: 3'-5' exonuclease]]

OCA at 09:30, 12 October 2017

OCA — Thu, 12 Oct 2017 09:30:58 GMT

←Older revision		Revision as of 09:30, 12 October 2017
Line 1:		Line 1:
		+
	==WRN exonuclease, Mg complex==		==WRN exonuclease, Mg complex==
	<StructureSection load='2fbx' size='340' side='right' caption='[[2fbx]], [[Resolution\|resolution]] 2.20Å' scene=''>		<StructureSection load='2fbx' size='340' side='right' caption='[[2fbx]], [[Resolution\|resolution]] 2.20Å' scene=''>
Line 6:		Line 7:
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2fbt\|2fbt]], [[2fbv\|2fbv]], [[2fby\|2fby]], [[2fc0\|2fc0]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2fbt\|2fbt]], [[2fbv\|2fbv]], [[2fby\|2fby]], [[2fc0\|2fc0]]</td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">WRN, RECQ3, RECQL2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">WRN, RECQ3, RECQL2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2fbx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2fbx OCA], [http://pdbe.org/2fbx PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2fbx RCSB], [http://www.ebi.ac.uk/pdbsum/2fbx PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2fbx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2fbx OCA], [http://pdbe.org/2fbx PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2fbx RCSB], [http://www.ebi.ac.uk/pdbsum/2fbx PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2fbx ProSAT]</span></td></tr>
	</table>		</table>
	== Disease ==		== Disease ==
Line 31:		Line 32:
	</div>		</div>
	<div class="pdbe-citations 2fbx" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 2fbx" style="background-color:#fffaf0;"></div>
-
-	==See Also==
-	*[[Helicase\|Helicase]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 10:01, 8 February 2016

OCA — Mon, 08 Feb 2016 10:01:30 GMT

←Older revision		Revision as of 10:01, 8 February 2016
Line 20:		Line 20:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2fbx ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 10:28, 10 September 2015

OCA — Thu, 10 Sep 2015 10:28:02 GMT

←Older revision		Revision as of 10:28, 10 September 2015
Line 2:		Line 2:
	<StructureSection load='2fbx' size='340' side='right' caption='[[2fbx]], [[Resolution\|resolution]] 2.20Å' scene=''>		<StructureSection load='2fbx' size='340' side='right' caption='[[2fbx]], [[Resolution\|resolution]] 2.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2fbx]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Homo_sapiens Homo sapiens~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FBX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2FBX FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2fbx]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FBX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2FBX FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2fbt\|2fbt]], [[2fbv\|2fbv]], [[2fby\|2fby]], [[2fc0\|2fc0]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2fbt\|2fbt]], [[2fbv\|2fbv]], [[2fby\|2fby]], [[2fc0\|2fc0]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">WRN, RECQ3, RECQL2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 ~~Homo sapiens~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">WRN, RECQ3, RECQL2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2fbx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2fbx OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2fbx RCSB], [http://www.ebi.ac.uk/pdbsum/2fbx PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2fbx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2fbx OCA], [http://pdbe.org/2fbx PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2fbx RCSB], [http://www.ebi.ac.uk/pdbsum/2fbx PDBsum]</span></td></tr>
	</table>		</table>
	== Disease ==		== Disease ==
Line 30:		Line 30:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 2fbx" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 37:		Line 38:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Homo sapiens~~]]	+	[[Category: Human]]
	[[Category: Perry, J J]]		[[Category: Perry, J J]]
	[[Category: 3'-5' exonuclease]]		[[Category: 3'-5' exonuclease]]

OCA at 08:32, 16 January 2015

OCA — Fri, 16 Jan 2015 08:32:50 GMT

←Older revision		Revision as of 08:32, 16 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2fbx]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FBX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2FBX FirstGlance]. <br>		<table><tr><td colspan='2'>[[2fbx]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FBX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2FBX FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2fbt\|2fbt]], [[2fbv\|2fbv]], [[2fby\|2fby]], [[2fc0\|2fc0]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2fbt\|2fbt]], [[2fbv\|2fbv]], [[2fby\|2fby]], [[2fc0\|2fc0]]</td></tr>
-	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">WRN, RECQ3, RECQL2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">WRN, RECQ3, RECQL2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2fbx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2fbx OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2fbx RCSB], [http://www.ebi.ac.uk/pdbsum/2fbx PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2fbx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2fbx OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2fbx RCSB], [http://www.ebi.ac.uk/pdbsum/2fbx PDBsum]</span></td></tr>
-	<table>	+	</table>
	== Disease ==		== Disease ==
	[[http://www.uniprot.org/uniprot/WRN_HUMAN WRN_HUMAN]] Defects in WRN are a cause of Werner syndrome (WRN) [MIM:[http://omim.org/entry/277700 277700]]. WRN is a rare autosomal recessive progeroid syndrome characterized by the premature onset of multiple age-related disorders, including atherosclerosis, cancer, non-insulin-dependent diabetes mellitus, ocular cataracts and osteoporosis. The major cause of death, at a median age of 47, is myocardial infarction. Currently all known WS mutations produces prematurely terminated proteins.<ref>PMID:16673358</ref> Defects in WRN may be a cause of colorectal cancer (CRC) [MIM:[http://omim.org/entry/114500 114500]].		[[http://www.uniprot.org/uniprot/WRN_HUMAN WRN_HUMAN]] Defects in WRN are a cause of Werner syndrome (WRN) [MIM:[http://omim.org/entry/277700 277700]]. WRN is a rare autosomal recessive progeroid syndrome characterized by the premature onset of multiple age-related disorders, including atherosclerosis, cancer, non-insulin-dependent diabetes mellitus, ocular cataracts and osteoporosis. The major cause of death, at a median age of 47, is myocardial infarction. Currently all known WS mutations produces prematurely terminated proteins.<ref>PMID:16673358</ref> Defects in WRN may be a cause of colorectal cancer (CRC) [MIM:[http://omim.org/entry/114500 114500]].
Line 38:		Line 38:
	</StructureSection>		</StructureSection>
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
-	[[Category: Perry, J J.]]	+	[[Category: Perry, J J]]
	[[Category: 3'-5' exonuclease]]		[[Category: 3'-5' exonuclease]]
	[[Category: Dnaq family]]		[[Category: Dnaq family]]

OCA at 00:24, 30 September 2014

OCA — Tue, 30 Sep 2014 00:24:17 GMT

←Older revision		Revision as of 00:24, 30 September 2014
Line 1:		Line 1:
-	~~{{STRUCTURE_2fbx~~\| ~~PDB~~=2fbx \| ~~SCENE~~= }}	+	==WRN exonuclease, Mg complex==
-	===WRN exonuclease, ~~Mg complex~~===	+	<StructureSection load='2fbx' size='340' side='right' caption='[[2fbx]], [[Resolution\|resolution]] 2.20Å' scene=''>
-	~~{{ABSTRACT_PUBMED_16622405}}~~	+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[2fbx]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FBX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2FBX FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2fbt\|2fbt]], [[2fbv\|2fbv]], [[2fby\|2fby]], [[2fc0\|2fc0]]</td></tr>
		+	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">WRN, RECQ3, RECQL2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2fbx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2fbx OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2fbx RCSB], [http://www.ebi.ac.uk/pdbsum/2fbx PDBsum]</span></td></tr>
		+	<table>
		+	== Disease ==
		+	[[http://www.uniprot.org/uniprot/WRN_HUMAN WRN_HUMAN]] Defects in WRN are a cause of Werner syndrome (WRN) [MIM:[http://omim.org/entry/277700 277700]]. WRN is a rare autosomal recessive progeroid syndrome characterized by the premature onset of multiple age-related disorders, including atherosclerosis, cancer, non-insulin-dependent diabetes mellitus, ocular cataracts and osteoporosis. The major cause of death, at a median age of 47, is myocardial infarction. Currently all known WS mutations produces prematurely terminated proteins.<ref>PMID:16673358</ref> Defects in WRN may be a cause of colorectal cancer (CRC) [MIM:[http://omim.org/entry/114500 114500]].
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/WRN_HUMAN WRN_HUMAN]] Multifunctional enzyme that has both magnesium and ATP-dependent DNA-helicase activity and 3'->5' exonuclease activity towards double-stranded DNA with a 5'-overhang. Has no nuclease activity towards single-stranded DNA or blunt-ended double-stranded DNA. Binds preferentially to DNA substrates containing alternate secondary structures, such as replication forks and Holliday junctions. May play an important role in the dissociation of joint DNA molecules that can arise as products of homologous recombination, at stalled replication forks or during DNA repair. Alleviates stalling of DNA polymerases at the site of DNA lesions. Important for genomic integrity. Plays a role in the formation of DNA replication focal centers; stably associates with foci elements generating binding sites for RP-A (By similarity).<ref>PMID:11863428</ref> <ref>PMID:17563354</ref> <ref>PMID:18596042</ref> <ref>PMID:19652551</ref> <ref>PMID:19283071</ref>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fb/2fbx_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	WRN is unique among the five human RecQ DNA helicases in having a functional exonuclease domain (WRN-exo) and being defective in the premature aging and cancer-related disorder Werner syndrome. Here, we characterize WRN-exo crystal structures, biochemical activity and participation in DNA end joining. Metal-ion complex structures, active site mutations and activity assays reveal a nuclease mechanism mediated by two metal ions. The DNA end-binding Ku70/80 complex specifically stimulates WRN-exo activity, and structure-based mutational inactivation of WRN-exo alters DNA end joining in human cells. We furthermore establish structural and biochemical similarities of WRN-exo to DnaQ-family replicative proofreading exonucleases, describing WRN-specific adaptations consistent with double-stranded DNA specificity and functionally important conformational changes. These results indicate WRN-exo is a human DnaQ family member and support DnaQ-like proofreading activities stimulated by Ku70/80, with implications for WRN functions in age-related pathologies and maintenance of genomic integrity.

-	~~==Disease==~~	+	WRN exonuclease structure and molecular mechanism imply an editing role in DNA end processing.,Perry JJ, Yannone SM, Holden LG, Hitomi C, Asaithamby A, Han S, Cooper PK, Chen DJ, Tainer JA Nat Struct Mol Biol. 2006 May;13(5):414-22. Epub 2006 Apr 23. PMID:16622405<ref>PMID:16622405</ref>
-	~~[[http://www.uniprot.org/uniprot/WRN_HUMAN WRN_HUMAN]] Defects~~ in ~~WRN are a cause of Werner syndrome (WRN) [MIM:[http://omim~~.~~org/entry/277700 277700]]. WRN is a rare autosomal recessive progeroid syndrome characterized by the premature onset of multiple age-related disorders~~, ~~including atherosclerosis~~, ~~cancer~~, ~~non-insulin-dependent diabetes mellitus~~, ~~ocular cataracts and osteoporosis. The major cause of death~~, ~~at a median age of 47~~, ~~is myocardial infarction~~. ~~Currently all known WS mutations produces prematurely terminated proteins~~.<ref>PMID:~~16673358~~</ref> ~~Defects in WRN may be a cause of colorectal cancer (CRC) [MIM:[http://omim.org/entry/114500 114500]].~~	+

-	~~==Function==~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[http:~~/~~/www.uniprot.org/uniprot/WRN_HUMAN WRN_HUMAN]] Multifunctional enzyme that has both magnesium and ATP-dependent DNA-helicase activity and 3'->5' exonuclease activity towards double-stranded DNA with~~ a 5'-overhang. Has no nuclease activity towards single-stranded DNA or blunt-ended double-stranded DNA. Binds preferentially to DNA substrates containing alternate secondary structures, such as replication forks and Holliday junctions. May play an important role in the dissociation of joint DNA molecules that can arise as products of homologous recombination, at stalled replication forks or during DNA repair. Alleviates stalling of ~~DNA polymerases at~~ the ~~site of DNA lesions~~. ~~Important for genomic integrity~~. ~~Plays a role in the formation~~ of ~~DNA replication focal centers; stably associates with foci elements generating binding sites for RP-A (By similarity)~~.<~~ref~~>~~PMID:11863428~~</~~ref~~>~~<ref>PMID:17563354</ref><ref>PMID:18596042</ref><ref>PMID:19652551</ref><ref>PMID:19283071</ref>~~	+	</div>
-		+
-	~~==About this Structure==~~	+
-	[[2fbx]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FBX OCA].	+

	==See Also==		==See Also==
	*[[Helicase\|Helicase]]		*[[Helicase\|Helicase]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	~~<ref group="xtra">PMID:016622405</ref>~~<references ~~group="xtra"~~/><~~references~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
	[[Category: Perry, J J.]]		[[Category: Perry, J J.]]

OCA at 10:10, 24 March 2013

OCA — Sun, 24 Mar 2013 10:10:55 GMT

←Older revision		Revision as of 10:10, 24 March 2013
Line 1:		Line 1:
-	[[Image:2fbx.png\|left\|200px]]
-
	{{STRUCTURE_2fbx\| PDB=2fbx \| SCENE= }}		{{STRUCTURE_2fbx\| PDB=2fbx \| SCENE= }}
-
	===WRN exonuclease, Mg complex===		===WRN exonuclease, Mg complex===
		+	{{ABSTRACT_PUBMED_16622405}}

-	~~{{ABSTRACT_PUBMED_16622405}}~~	+	==Disease==
		+	[[http://www.uniprot.org/uniprot/WRN_HUMAN WRN_HUMAN]] Defects in WRN are a cause of Werner syndrome (WRN) [MIM:[http://omim.org/entry/277700 277700]]. WRN is a rare autosomal recessive progeroid syndrome characterized by the premature onset of multiple age-related disorders, including atherosclerosis, cancer, non-insulin-dependent diabetes mellitus, ocular cataracts and osteoporosis. The major cause of death, at a median age of 47, is myocardial infarction. Currently all known WS mutations produces prematurely terminated proteins.<ref>PMID:16673358</ref> Defects in WRN may be a cause of colorectal cancer (CRC) [MIM:[http://omim.org/entry/114500 114500]].
		+
		+	==Function==
		+	[[http://www.uniprot.org/uniprot/WRN_HUMAN WRN_HUMAN]] Multifunctional enzyme that has both magnesium and ATP-dependent DNA-helicase activity and 3'->5' exonuclease activity towards double-stranded DNA with a 5'-overhang. Has no nuclease activity towards single-stranded DNA or blunt-ended double-stranded DNA. Binds preferentially to DNA substrates containing alternate secondary structures, such as replication forks and Holliday junctions. May play an important role in the dissociation of joint DNA molecules that can arise as products of homologous recombination, at stalled replication forks or during DNA repair. Alleviates stalling of DNA polymerases at the site of DNA lesions. Important for genomic integrity. Plays a role in the formation of DNA replication focal centers; stably associates with foci elements generating binding sites for RP-A (By similarity).<ref>PMID:11863428</ref><ref>PMID:17563354</ref><ref>PMID:18596042</ref><ref>PMID:19652551</ref><ref>PMID:19283071</ref>

	==About this Structure==		==About this Structure==
Line 14:		Line 16:

	==Reference==		==Reference==
-	<ref group="xtra">PMID:016622405</ref><references group="xtra"/>	+	<ref group="xtra">PMID:016622405</ref><references group="xtra"/><references/>
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
	[[Category: Perry, J J.]]		[[Category: Perry, J J.]]

OCA at 09:47, 20 October 2012

OCA — Sat, 20 Oct 2012 09:47:24 GMT

←Older revision		Revision as of 09:47, 20 October 2012
Line 8:		Line 8:

	==About this Structure==		==About this Structure==
-	[[2fbx]] is a 1 chain structure ~~of [[Helicase]]~~ with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FBX OCA].	+	[[2fbx]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FBX OCA].

	==See Also==		==See Also==

OCA: Protected "2fbx" [edit=sysop:move=sysop]

OCA — Wed, 25 Jul 2012 23:57:47 GMT

Protected "2fbx" [edit=sysop:move=sysop]

←Older revision

Revision as of 23:57, 25 July 2012