2fhs - Revision history

OCA at 09:23, 14 February 2024

OCA — Wed, 14 Feb 2024 09:23:31 GMT

←Older revision		Revision as of 09:23, 14 February 2024
Line 3:		Line 3:
	<StructureSection load='2fhs' size='340' side='right'caption='[[2fhs]], [[Resolution\|resolution]] 2.70Å' scene=''>		<StructureSection load='2fhs' size='340' side='right'caption='[[2fhs]], [[Resolution\|resolution]] 2.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2fhs]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/~~"bacillus_coli"_migula_1895 "bacillus~~ coli~~" migula 1895~~] and [https://en.wikipedia.org/wiki/~~Ecow3 Ecow3~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FHS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2FHS FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2fhs]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] and [https://en.wikipedia.org/wiki/Escherichia_coli_str._K-12_substr._W3110 Escherichia coli str. K-12 substr. W3110]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FHS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2FHS FirstGlance]. <br>
-	</td></tr><tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat"~~>acpP ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&~~id=~~562~~ "~~Bacillus coli~~" ~~Migula 1895])</td~~>~~</tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.7Å</td></tr>
-	~~<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Enoyl~~-[~~acyl-carrier-protein]_reductase_(NADH) Enoyl-~~[~~acyl-carrier-protein~~] ~~reductase (NADH)~~]~~, with EC number [https://www~~.~~brenda-enzymes.info/php/result_flat.php4?ecno=1.3.1.9 1.3.1.9] </span>~~</td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2fhs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2fhs OCA], [https://pdbe.org/2fhs PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2fhs RCSB], [https://www.ebi.ac.uk/pdbsum/2fhs PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2fhs ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2fhs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2fhs OCA], [https://pdbe.org/2fhs PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2fhs RCSB], [https://www.ebi.ac.uk/pdbsum/2fhs PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2fhs ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/~~ACP_ECOLI ACP_ECOLI~~]~~] Carrier~~ of the ~~growing~~ fatty acid ~~chain~~ in ~~fatty acid~~ biosynthesis.~~[HAMAP-Rule~~:~~MF_01217]~~	+	[https://www.uniprot.org/uniprot/FABI_ECOLI FABI_ECOLI] Catalyzes the reduction of a carbon-carbon double bond in an enoyl moiety that is covalently linked to an acyl carrier protein (ACP). Involved in the elongation cycle of fatty acid which are used in the lipid metabolism and in the biotin biosynthesis.<ref>PMID:8119879</ref> <ref>PMID:7592873</ref> <ref>PMID:20693992</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 20:		Line 19:
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2fhs ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2fhs ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	Acyl carrier proteins play a central role in metabolism by transporting substrates in a wide variety of pathways including the biosynthesis of fatty acids and polyketides. However, despite their importance, there is a paucity of direct structural information concerning the interaction of ACPs with enzymes in these pathways. Here we report the structure of an acyl-ACP substrate bound to the Escherichia coli fatty acid biosynthesis enoyl reductase enzyme (FabI), based on a combination of x-ray crystallography and molecular dynamics simulation. The structural data are in agreement with kinetic studies on wild-type and mutant FabIs, and reveal that the complex is primarily stabilized by interactions between acidic residues in the ACP helix alpha2 and a patch of basic residues adjacent to the FabI substrate-binding loop. Unexpectedly, the acyl-pantetheine thioester carbonyl is not hydrogen-bonded to Tyr(156), a conserved component of the short chain alcohol dehydrogenase/reductase superfamily active site triad. FabI is a proven target for drug discovery and the present structure provides insight into the molecular determinants that regulate the interaction of ACPs with target proteins.
-
-	Structure of acyl carrier protein bound to FabI, the FASII enoyl reductase from Escherichia coli.,Rafi S, Novichenok P, Kolappan S, Zhang X, Stratton CF, Rawat R, Kisker C, Simmerling C, Tonge PJ J Biol Chem. 2006 Dec 22;281(51):39285-93. Epub 2006 Sep 29. PMID:17012233<ref>PMID:17012233</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 2fhs" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
	*[[Acyl carrier protein 3D structures\|Acyl carrier protein 3D structures]]		*[[Acyl carrier protein 3D structures\|Acyl carrier protein 3D structures]]
	*[[Enoyl-Acyl-Carrier Protein Reductase 3D structures\|Enoyl-Acyl-Carrier Protein Reductase 3D structures]]		*[[Enoyl-Acyl-Carrier Protein Reductase 3D structures\|Enoyl-Acyl-Carrier Protein Reductase 3D structures]]
-	*[[Fatty acid synthase 3D structures\|Fatty acid synthase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Bacillus~~ coli ~~migula 1895~~]]	+	[[Category: Escherichia coli]]
-	[[Category: ~~Ecow3~~]]	+	[[Category: Escherichia coli str. K-12 substr. W3110]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Kisker, C]]	+	[[Category: Kisker C]]
-	[[Category: Kolappan, S]]	+	[[Category: Kolappan S]]
-	[[Category: Novichenok, P]]	+	[[Category: Novichenok P]]
-	[[Category: Rafi, S]]	+	[[Category: Rafi S]]
-	[[Category: Simmerling, C]]	+	[[Category: Simmerling C]]
-	[[Category: Tonge~~, P J]]~~	+	[[Category: Tonge PJ]]
-	~~[[Category: Oxidoreductase-biosynthetic protein complex]]~~	+
-	~~[[Category: Protein-protein complex~~]]	+

OCA at 15:27, 3 March 2021

OCA — Wed, 03 Mar 2021 15:27:11 GMT

←Older revision		Revision as of 15:27, 3 March 2021
Line 1:		Line 1:

	==Structure of Acyl Carrier Protein Bound to FabI, the Enoyl Reductase from Escherichia Coli==		==Structure of Acyl Carrier Protein Bound to FabI, the Enoyl Reductase from Escherichia Coli==
-	<StructureSection load='2fhs' size='340' side='right' caption='[[2fhs]], [[Resolution\|resolution]] 2.70Å' scene=''>	+	<StructureSection load='2fhs' size='340' side='right'caption='[[2fhs]], [[Resolution\|resolution]] 2.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2fhs]] is a 3 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895] and [~~http~~://en.wikipedia.org/wiki/Ecow3 Ecow3]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FHS OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2FHS FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2fhs]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895] and [https://en.wikipedia.org/wiki/Ecow3 Ecow3]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FHS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2FHS FirstGlance]. <br>
-	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">acpP ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 "Bacillus coli" Migula 1895])</td></tr>	+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">acpP ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 "Bacillus coli" Migula 1895])</td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Enoyl-[acyl-carrier-protein]_reductase_(NADH) Enoyl-[acyl-carrier-protein] reductase (NADH)], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.3.1.9 1.3.1.9] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Enoyl-[acyl-carrier-protein]_reductase_(NADH) Enoyl-[acyl-carrier-protein] reductase (NADH)], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.3.1.9 1.3.1.9] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2fhs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2fhs OCA], [~~http~~://pdbe.org/2fhs PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2fhs RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2fhs PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2fhs ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2fhs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2fhs OCA], [https://pdbe.org/2fhs PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2fhs RCSB], [https://www.ebi.ac.uk/pdbsum/2fhs PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2fhs ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/ACP_ECOLI ACP_ECOLI]] Carrier of the growing fatty acid chain in fatty acid biosynthesis.[HAMAP-Rule:MF_01217]	+	[[https://www.uniprot.org/uniprot/ACP_ECOLI ACP_ECOLI]] Carrier of the growing fatty acid chain in fatty acid biosynthesis.[HAMAP-Rule:MF_01217]
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 31:		Line 31:

	==See Also==		==See Also==
-	*[[Acyl carrier protein\|Acyl carrier protein]]	+	*[[Acyl carrier protein 3D structures\|Acyl carrier protein 3D structures]]
-	*[[Enoyl-Acyl-Carrier Protein Reductase\|Enoyl-Acyl-Carrier Protein Reductase]]	+	*[[Enoyl-Acyl-Carrier Protein Reductase 3D structures\|Enoyl-Acyl-Carrier Protein Reductase 3D structures]]
-	*[[Fatty acid synthase\|Fatty acid synthase]]	+	*[[Fatty acid synthase 3D structures\|Fatty acid synthase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 40:		Line 40:
	[[Category: Bacillus coli migula 1895]]		[[Category: Bacillus coli migula 1895]]
	[[Category: Ecow3]]		[[Category: Ecow3]]
		+	[[Category: Large Structures]]
	[[Category: Kisker, C]]		[[Category: Kisker, C]]
	[[Category: Kolappan, S]]		[[Category: Kolappan, S]]

OCA at 09:11, 14 June 2018

OCA — Thu, 14 Jun 2018 09:11:59 GMT

←Older revision		Revision as of 09:11, 14 June 2018
Line 1:		Line 1:
		+
	==Structure of Acyl Carrier Protein Bound to FabI, the Enoyl Reductase from Escherichia Coli==		==Structure of Acyl Carrier Protein Bound to FabI, the Enoyl Reductase from Escherichia Coli==
	<StructureSection load='2fhs' size='340' side='right' caption='[[2fhs]], [[Resolution\|resolution]] 2.70Å' scene=''>		<StructureSection load='2fhs' size='340' side='right' caption='[[2fhs]], [[Resolution\|resolution]] 2.70Å' scene=''>
Line 5:		Line 6:
	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">acpP ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 "Bacillus coli" Migula 1895])</td></tr>		</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">acpP ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 "Bacillus coli" Migula 1895])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Enoyl-[acyl-carrier-protein]_reductase_(NADH) Enoyl-[acyl-carrier-protein] reductase (NADH)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.3.1.9 1.3.1.9] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Enoyl-[acyl-carrier-protein]_reductase_(NADH) Enoyl-[acyl-carrier-protein] reductase (NADH)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.3.1.9 1.3.1.9] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2fhs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2fhs OCA], [http://pdbe.org/2fhs PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2fhs RCSB], [http://www.ebi.ac.uk/pdbsum/2fhs PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2fhs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2fhs OCA], [http://pdbe.org/2fhs PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2fhs RCSB], [http://www.ebi.ac.uk/pdbsum/2fhs PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2fhs ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 13:		Line 14:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fh/2fhs_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fh/2fhs_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2fhs ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 22:30, 11 September 2015

OCA — Fri, 11 Sep 2015 22:30:08 GMT

←Older revision		Revision as of 22:30, 11 September 2015
Line 2:		Line 2:
	<StructureSection load='2fhs' size='340' side='right' caption='[[2fhs]], [[Resolution\|resolution]] 2.70Å' scene=''>		<StructureSection load='2fhs' size='340' side='right' caption='[[2fhs]], [[Resolution\|resolution]] 2.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2fhs]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Escherichia_coli Escherichia~~ coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FHS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2FHS FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2fhs]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895] and [http://en.wikipedia.org/wiki/Ecow3 Ecow3]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FHS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2FHS FirstGlance]. <br>
-	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">acpP ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 ~~Escherichia~~ coli])</td></tr>	+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">acpP ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 "Bacillus coli" Migula 1895])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Enoyl-[acyl-carrier-protein]_reductase_(NADH) Enoyl-[acyl-carrier-protein] reductase (NADH)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.3.1.9 1.3.1.9] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Enoyl-[acyl-carrier-protein]_reductase_(NADH) Enoyl-[acyl-carrier-protein] reductase (NADH)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.3.1.9 1.3.1.9] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2fhs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2fhs OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2fhs RCSB], [http://www.ebi.ac.uk/pdbsum/2fhs PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2fhs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2fhs OCA], [http://pdbe.org/2fhs PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2fhs RCSB], [http://www.ebi.ac.uk/pdbsum/2fhs PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 27:		Line 27:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 2fhs" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 36:		Line 37:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Escherichia~~ coli]]	+	[[Category: Bacillus coli migula 1895]]
		+	[[Category: Ecow3]]
	[[Category: Kisker, C]]		[[Category: Kisker, C]]
	[[Category: Kolappan, S]]		[[Category: Kolappan, S]]

OCA at 20:18, 25 December 2014

OCA — Thu, 25 Dec 2014 20:18:33 GMT

←Older revision		Revision as of 20:18, 25 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2fhs]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FHS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2FHS FirstGlance]. <br>		<table><tr><td colspan='2'>[[2fhs]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FHS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2FHS FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">acpP ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])</td></tr>	+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">acpP ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])</td></tr>
-	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Enoyl-[acyl-carrier-protein]_reductase_(NADH) Enoyl-[acyl-carrier-protein] reductase (NADH)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.3.1.9 1.3.1.9] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Enoyl-[acyl-carrier-protein]_reductase_(NADH) Enoyl-[acyl-carrier-protein] reductase (NADH)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.3.1.9 1.3.1.9] </span></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2fhs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2fhs OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2fhs RCSB], [http://www.ebi.ac.uk/pdbsum/2fhs PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2fhs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2fhs OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2fhs RCSB], [http://www.ebi.ac.uk/pdbsum/2fhs PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/ACP_ECOLI ACP_ECOLI]] Carrier of the growing fatty acid chain in fatty acid biosynthesis.[HAMAP-Rule:MF_01217]
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 35:		Line 37:
	</StructureSection>		</StructureSection>
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
-	[[Category: Kisker, C.]]	+	[[Category: Kisker, C]]
-	[[Category: Kolappan, S.]]	+	[[Category: Kolappan, S]]
-	[[Category: Novichenok, P.]]	+	[[Category: Novichenok, P]]
-	[[Category: Rafi, S.]]	+	[[Category: Rafi, S]]
-	[[Category: Simmerling, C.]]	+	[[Category: Simmerling, C]]
-	[[Category: Tonge, P J.]]	+	[[Category: Tonge, P J]]
	[[Category: Oxidoreductase-biosynthetic protein complex]]		[[Category: Oxidoreductase-biosynthetic protein complex]]
	[[Category: Protein-protein complex]]		[[Category: Protein-protein complex]]

OCA at 03:41, 29 September 2014

OCA — Mon, 29 Sep 2014 03:41:04 GMT

←Older revision		Revision as of 03:41, 29 September 2014
Line 1:		Line 1:
-	[[~~Image~~:2fhs.~~png\|left~~\|200px]]	+	==Structure of Acyl Carrier Protein Bound to FabI, the Enoyl Reductase from Escherichia Coli==
		+	<StructureSection load='2fhs' size='340' side='right' caption='[[2fhs]], [[Resolution\|resolution]] 2.70Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[2fhs]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FHS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2FHS FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">acpP ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])</td></tr>
		+	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Enoyl-[acyl-carrier-protein]_reductase_(NADH) Enoyl-[acyl-carrier-protein] reductase (NADH)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.3.1.9 1.3.1.9] </span></td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2fhs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2fhs OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2fhs RCSB], [http://www.ebi.ac.uk/pdbsum/2fhs PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fh/2fhs_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Acyl carrier proteins play a central role in metabolism by transporting substrates in a wide variety of pathways including the biosynthesis of fatty acids and polyketides. However, despite their importance, there is a paucity of direct structural information concerning the interaction of ACPs with enzymes in these pathways. Here we report the structure of an acyl-ACP substrate bound to the Escherichia coli fatty acid biosynthesis enoyl reductase enzyme (FabI), based on a combination of x-ray crystallography and molecular dynamics simulation. The structural data are in agreement with kinetic studies on wild-type and mutant FabIs, and reveal that the complex is primarily stabilized by interactions between acidic residues in the ACP helix alpha2 and a patch of basic residues adjacent to the FabI substrate-binding loop. Unexpectedly, the acyl-pantetheine thioester carbonyl is not hydrogen-bonded to Tyr(156), a conserved component of the short chain alcohol dehydrogenase/reductase superfamily active site triad. FabI is a proven target for drug discovery and the present structure provides insight into the molecular determinants that regulate the interaction of ACPs with target proteins.

-	~~{{STRUCTURE_2fhs\| PDB=2fhs \| SCENE= }}~~	+	Structure of acyl carrier protein bound to FabI, the FASII enoyl reductase from Escherichia coli.,Rafi S, Novichenok P, Kolappan S, Zhang X, Stratton CF, Rawat R, Kisker C, Simmerling C, Tonge PJ J Biol Chem. 2006 Dec 22;281(51):39285-93. Epub 2006 Sep 29. PMID:17012233<ref>PMID:17012233</ref>

-	~~===Structure~~ of ~~Acyl Carrier Protein Bound to FabI,~~ the ~~Enoyl Reductase from Escherichia Coli===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_17012233}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[2fhs]] is a 3 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Escherichia_coli Escherichia coli]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=2FHS OCA].~~	+

	==See Also==		==See Also==
		+	*[[Acyl carrier protein\|Acyl carrier protein]]
	*[[Enoyl-Acyl-Carrier Protein Reductase\|Enoyl-Acyl-Carrier Protein Reductase]]		*[[Enoyl-Acyl-Carrier Protein Reductase\|Enoyl-Acyl-Carrier Protein Reductase]]
	*[[Fatty acid synthase\|Fatty acid synthase]]		*[[Fatty acid synthase\|Fatty acid synthase]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	<~~ref group="xtra">PMID:017012233<~~/~~ref~~><~~references group="xtra"~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
	[[Category: Kisker, C.]]		[[Category: Kisker, C.]]

OCA at 21:01, 20 October 2012

OCA — Sat, 20 Oct 2012 21:01:49 GMT

←Older revision		Revision as of 21:01, 20 October 2012
Line 8:		Line 8:

	==About this Structure==		==About this Structure==
-	[[2fhs]] is a 3 chain structure ~~of [[Enoyl-Acyl-Carrier Protein Reductase]] and [[Fatty acid synthase]]~~ with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FHS OCA].	+	[[2fhs]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FHS OCA].

	==See Also==		==See Also==

OCA: Protected "2fhs" [edit=sysop:move=sysop]

OCA — Fri, 27 Jul 2012 12:22:12 GMT

Protected "2fhs" [edit=sysop:move=sysop]

←Older revision

Revision as of 12:22, 27 July 2012

OCA at 12:22, 27 July 2012

OCA — Fri, 27 Jul 2012 12:22:10 GMT

←Older revision		Revision as of 12:22, 27 July 2012
Line 1:		Line 1:
-	{{Seed}}
	[[Image:2fhs.png\|left\|200px]]		[[Image:2fhs.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_2fhs", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_2fhs\| PDB=2fhs \| SCENE= }}		{{STRUCTURE_2fhs\| PDB=2fhs \| SCENE= }}

	===Structure of Acyl Carrier Protein Bound to FabI, the Enoyl Reductase from Escherichia Coli===		===Structure of Acyl Carrier Protein Bound to FabI, the Enoyl Reductase from Escherichia Coli===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_17012233}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 17012233 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_17012233}}		{{ABSTRACT_PUBMED_17012233}}

	==About this Structure==		==About this Structure==
-	~~2FHS~~ is a 3 ~~chains~~ structure of ~~sequences from~~ [~~http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli~~] and [http://en.wikipedia.org/wiki/~~Escherichia_coli_w3110~~ Escherichia coli ~~w3110~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FHS OCA].	+	[[2fhs]] is a 3 chain structure of [[Enoyl-Acyl-Carrier Protein Reductase]] and [[Fatty acid synthase]] with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FHS OCA].
		+
		+	==See Also==
		+	*[[Enoyl-Acyl-Carrier Protein Reductase\|Enoyl-Acyl-Carrier Protein Reductase]]
		+	*[[Fatty acid synthase\|Fatty acid synthase]]

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~17012233~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:017012233</ref><references group="xtra"/>
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
-	[[Category: Escherichia coli w3110]]
	[[Category: Kisker, C.]]		[[Category: Kisker, C.]]
	[[Category: Kolappan, S.]]		[[Category: Kolappan, S.]]
Line 32:		Line 23:
	[[Category: Simmerling, C.]]		[[Category: Simmerling, C.]]
	[[Category: Tonge, P J.]]		[[Category: Tonge, P J.]]
		+	[[Category: Oxidoreductase-biosynthetic protein complex]]
	[[Category: Protein-protein complex]]		[[Category: Protein-protein complex]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Feb 18 05:57:24 2009''

OCA at 03:57, 18 February 2009

OCA — Wed, 18 Feb 2009 03:57:26 GMT

←Older revision		Revision as of 03:57, 18 February 2009
Line 20:		Line 20:

	==About this Structure==		==About this Structure==
-	2FHS is a ~~[[Protein complex]]~~ structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] and [http://en.wikipedia.org/wiki/Escherichia_coli_w3110 Escherichia coli w3110]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FHS OCA].	+	2FHS is a 3 chains structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] and [http://en.wikipedia.org/wiki/Escherichia_coli_w3110 Escherichia coli w3110]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FHS OCA].

	==Reference==		==Reference==
-	Structure of acyl carrier protein bound to FabI, the FASII enoyl reductase from Escherichia coli., Rafi S, Novichenok P, Kolappan S, Zhang X, Stratton CF, Rawat R, Kisker C, Simmerling C, Tonge PJ, J Biol Chem. 2006 Dec 22;281(51):39285-93. Epub 2006 Sep 29. PMID:~~[http:~~//~~www.ncbi.nlm.nih.gov/pubmed/17012233 17012233]~~	+	<ref group="xtra">PMID:17012233</ref><references group="xtra"/>
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
	[[Category: Escherichia coli w3110]]		[[Category: Escherichia coli w3110]]
-	[[Category: Protein complex]]
	[[Category: Kisker, C.]]		[[Category: Kisker, C.]]
	[[Category: Kolappan, S.]]		[[Category: Kolappan, S.]]
Line 35:		Line 34:
	[[Category: Protein-protein complex]]		[[Category: Protein-protein complex]]

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Tue Jul 29 12~~:41:~~43 2008~~''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Feb 18 05:57:24 2009''