2fwx - Revision history

OCA at 14:37, 29 December 2021

OCA — Wed, 29 Dec 2021 14:37:53 GMT

←Older revision		Revision as of 14:37, 29 December 2021
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	<StructureSection load='2fwx' size='340' side='right'caption='[[2fwx]]' scene=''>		<StructureSection load='2fwx' size='340' side='right'caption='[[2fwx]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2FWX FirstGlance]. <br>	+	<table><tr><td colspan='2'>For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2FWX FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2fwx FirstGlance], [~~http~~://www.ebi.ac.uk/pdbsum/2fwx PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2fwx ProSAT]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2fwx FirstGlance], [https://www.ebi.ac.uk/pdbsum/2fwx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2fwx ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 11:06, 27 March 2020

OCA — Fri, 27 Mar 2020 11:06:25 GMT

←Older revision		Revision as of 11:06, 27 March 2020
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	==HOMOLOGY MODEL OF BACILLUS SUBTILIS GLUTAMINE SYNTHETASE==		==HOMOLOGY MODEL OF BACILLUS SUBTILIS GLUTAMINE SYNTHETASE==
-	<StructureSection load='2fwx' size='340' side='right' caption='[[2fwx]]' scene=''>	+	<StructureSection load='2fwx' size='340' side='right'caption='[[2fwx]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2FWX FirstGlance]. <br>		<table><tr><td colspan='2'>For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2FWX FirstGlance]. <br>
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	</StructureSection>		</StructureSection>
	[[Category: Theoretical Model]]		[[Category: Theoretical Model]]
		+	[[Category: Large Structures]]
	[[Category: Fisher Jr, S H]]		[[Category: Fisher Jr, S H]]
	[[Category: Wray, L V]]		[[Category: Wray, L V]]

OCA at 07:05, 20 June 2018

OCA — Wed, 20 Jun 2018 07:05:38 GMT

←Older revision		Revision as of 07:05, 20 June 2018
Line 5:		Line 5:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2FWX FirstGlance]. <br>		<table><tr><td colspan='2'>For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2FWX FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2fwx FirstGlance], [http://www.ebi.ac.uk/pdbsum/2fwx PDBsum]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2fwx FirstGlance], [http://www.ebi.ac.uk/pdbsum/2fwx PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2fwx ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 10:37, 11 February 2016

OCA — Thu, 11 Feb 2016 10:37:29 GMT

←Older revision		Revision as of 10:37, 11 February 2016
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		+	{{Theoretical_model}}
		+
	==HOMOLOGY MODEL OF BACILLUS SUBTILIS GLUTAMINE SYNTHETASE==		==HOMOLOGY MODEL OF BACILLUS SUBTILIS GLUTAMINE SYNTHETASE==
	<StructureSection load='2fwx' size='340' side='right' caption='[[2fwx]]' scene=''>		<StructureSection load='2fwx' size='340' side='right' caption='[[2fwx]]' scene=''>
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Theoretical Model]]
	[[Category: Fisher Jr, S H]]		[[Category: Fisher Jr, S H]]
	[[Category: Wray, L V]]		[[Category: Wray, L V]]

OCA at 01:49, 11 September 2015

OCA — Fri, 11 Sep 2015 01:49:50 GMT

←Older revision		Revision as of 01:49, 11 September 2015
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	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 2fwx" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 08:20, 16 January 2015

OCA — Fri, 16 Jan 2015 08:20:56 GMT

←Older revision		Revision as of 08:20, 16 January 2015
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2FWX FirstGlance]. <br>		<table><tr><td colspan='2'>For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2FWX FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2fwx FirstGlance], [http://www.ebi.ac.uk/pdbsum/2fwx PDBsum]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2fwx FirstGlance], [http://www.ebi.ac.uk/pdbsum/2fwx PDBsum]</span></td></tr>
-	<table>	+	</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==

OCA at 04:39, 29 September 2014

OCA — Mon, 29 Sep 2014 04:39:15 GMT

←Older revision		Revision as of 04:39, 29 September 2014
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-	~~{{Theoretical_model}}~~	+	==HOMOLOGY MODEL OF BACILLUS SUBTILIS GLUTAMINE SYNTHETASE==
		+	<StructureSection load='2fwx' size='340' side='right' caption='[[2fwx]]' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2FWX FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2fwx FirstGlance], [http://www.ebi.ac.uk/pdbsum/2fwx PDBsum]</span></td></tr>
		+	<table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The feedback-inhibited form of Bacillus subtilis glutamine synthetase regulates the activity of the TnrA transcription factor through a protein-protein interaction that prevents TnrA from binding to DNA. Five mutants containing feedback-resistant glutamine synthetases (E65G, S66P, M68I, H195Y, and P318S) were isolated by screening for colonies capable of cross-feeding Gln(-) cells. In vitro enzymatic assays revealed that the mutant enzymes had increased resistance to inhibition by glutamine, AMP, and methionine sulfoximine. The mutant proteins had a variety of enzymatic alterations that included changes in the levels of enzymatic activity and in substrate K(m) values. Constitutive expression of TnrA- and GlnR-regulated genes was seen in all five mutants. In gel mobility shift assays, the E65G and S66P enzymes were unable to inhibit TnrA DNA binding, while the other three mutant proteins (M68I, H195Y, and P318S) showed partial inhibition of TnrA DNA binding. A homology model of B. subtilis glutamine synthetase revealed that the five mutated amino acid residues are located in the enzyme active site. These observations are consistent with the hypothesis that glutamine and AMP bind at the active site to bring about feedback inhibition of glutamine synthetase.

-	~~[[Image~~:~~2fwx~~.~~png\|left\|200px]]~~	+	Feedback-resistant mutations in Bacillus subtilis glutamine synthetase are clustered in the active site.,Fisher SH, Wray LV Jr J Bacteriol. 2006 Aug;188(16):5966-74. PMID:16885465<ref>PMID:16885465</ref>

-	~~{{STRUCTURE_2fwx\| PDB=2fwx \| SCENE= }}~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	==~~=HOMOLOGY MODEL OF BACILLUS SUBTILIS GLUTAMINE SYNTHETASE===~~	+	== References ==
-		+	<references/>
-	~~{{ABSTRACT_PUBMED_16885465}}~~	+	__TOC__
-		+	</StructureSection>
-	~~==Reference~~==	+
-	<~~ref group="xtra">PMID:016885465<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Fisher Jr, S H]]		[[Category: Fisher Jr, S H]]
	[[Category: Wray, L V]]		[[Category: Wray, L V]]

OCA: Protected "2fwx" [edit=sysop:move=sysop]

OCA — Sun, 21 Oct 2012 09:02:28 GMT

Protected "2fwx" [edit=sysop:move=sysop]

←Older revision

Revision as of 09:02, 21 October 2012

OCA at 09:02, 21 October 2012

OCA — Sun, 21 Oct 2012 09:02:25 GMT

←Older revision		Revision as of 09:02, 21 October 2012
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	{{Theoretical_model}}		{{Theoretical_model}}
-	~~{{Seed}}~~	+
	[[Image:2fwx.png\|left\|200px]]		[[Image:2fwx.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_2fwx", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_2fwx\| PDB=2fwx \| SCENE= }}		{{STRUCTURE_2fwx\| PDB=2fwx \| SCENE= }}

	===HOMOLOGY MODEL OF BACILLUS SUBTILIS GLUTAMINE SYNTHETASE===		===HOMOLOGY MODEL OF BACILLUS SUBTILIS GLUTAMINE SYNTHETASE===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_16885465}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 16885465 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_16885465}}		{{ABSTRACT_PUBMED_16885465}}
-
-	==About this Structure==
-	Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FWX OCA].

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~16885465~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:016885465</ref><references group="xtra"/>
	[[Category: Fisher Jr, S H]]		[[Category: Fisher Jr, S H]]
	[[Category: Wray, L V]]		[[Category: Wray, L V]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Apr 8 07:32:17 2010''

OCA at 04:32, 8 April 2010

OCA — Thu, 08 Apr 2010 04:32:18 GMT

←Older revision		Revision as of 04:32, 8 April 2010
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-	~~'''Theoretical Model'''~~	+	{{Theoretical_model}}
		+	{{Seed}}
		+	[[Image:2fwx.png\|left\|200px]]

-	The ~~entry 2FWX~~ is ~~a Theoretical Model titled 'Homology Model of Bacillus subtilis Glutamine Synthetase'~~.	+	<!--
		+	The line below this paragraph, containing "STRUCTURE_2fwx", creates the "Structure Box" on the page.
		+	You may change the PDB parameter (which sets the PDB file loaded into the applet)
		+	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
		+	or leave the SCENE parameter empty for the default display.
		+	-->
		+	{{STRUCTURE_2fwx\| PDB=2fwx \| SCENE= }}

-	~~[[Category:Theoretical Model]]~~	+	===HOMOLOGY MODEL OF BACILLUS SUBTILIS GLUTAMINE SYNTHETASE===


-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Wed Jul 30 09~~:56:~~42 2008~~''	+	<!--
		+	The line below this paragraph, {{ABSTRACT_PUBMED_16885465}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 16885465 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_16885465}}
		+
		+	==About this Structure==
		+	Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FWX OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:16885465</ref><references group="xtra"/>
		+	[[Category: Fisher Jr, S H]]
		+	[[Category: Wray, L V]]
		+
		+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Apr 8 07:32:17 2010''