2g38 - Revision history

OCA at 09:26, 14 February 2024

OCA — Wed, 14 Feb 2024 09:26:19 GMT

←Older revision		Revision as of 09:26, 14 February 2024
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	<StructureSection load='2g38' size='340' side='right'caption='[[2g38]], [[Resolution\|resolution]] 2.20Å' scene=''>		<StructureSection load='2g38' size='340' side='right'caption='[[2g38]], [[Resolution\|resolution]] 2.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2g38]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Myctu Myctu~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2G38 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2G38 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2g38]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis_H37Rv Mycobacterium tuberculosis H37Rv]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2G38 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2G38 FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.2Å</td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat">~~Rv2431c ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode~~=~~Info&srchmode~~=~~5&id=83332 MYCTU]~~)~~, Rv2430c ([https:~~/~~/www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83332 MYCTU])~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2g38 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2g38 OCA], [https://pdbe.org/2g38 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2g38 RCSB], [https://www.ebi.ac.uk/pdbsum/2g38 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2g38 ProSAT], [https://www.topsan.org/Proteins/ISFI/2g38 TOPSAN]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2g38 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2g38 OCA], [https://pdbe.org/2g38 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2g38 RCSB], [https://www.ebi.ac.uk/pdbsum/2g38 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2g38 ProSAT], [https://www.topsan.org/Proteins/ISFI/2g38 TOPSAN]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/Q7D756_MYCTO Q7D756_MYCTO]
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2g38 ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2g38 ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	The developing science called structural genomics has focused to date mainly on high-throughput expression of individual proteins, followed by their purification and structure determination. In contrast, the term structural biology is used to denote the determination of structures, often complexes of several macromolecules, that illuminate aspects of biological function. Here we bridge structural genomics to structural biology with a procedure for determining protein complexes of previously unknown function from any organism with a sequenced genome. From computational genomic analysis, we identify functionally linked proteins and verify their interaction in vitro by coexpression/copurification. We illustrate this procedure by the structural determination of a previously unknown complex between a PE and PPE protein from the Mycobacterium tuberculosis genome, members of protein families that constitute approximately 10% of the coding capacity of this genome. The predicted complex was readily expressed, purified, and crystallized, although we had previously failed in expressing individual PE and PPE proteins on their own. The reason for the failure is clear from the structure, which shows that the PE and PPE proteins mate along an extended apolar interface to form a four-alpha-helical bundle, where two of the alpha-helices are contributed by the PE protein and two by the PPE protein. Our entire procedure for the identification, characterization, and structural determination of protein complexes can be scaled to a genome-wide level.
-
-	Toward the structural genomics of complexes: crystal structure of a PE/PPE protein complex from Mycobacterium tuberculosis.,Strong M, Sawaya MR, Wang S, Phillips M, Cascio D, Eisenberg D Proc Natl Acad Sci U S A. 2006 May 23;103(21):8060-5. Epub 2006 May 11. PMID:16690741<ref>PMID:16690741</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 2g38" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
	*[[PE/PPE Protein Complex\|PE/PPE Protein Complex]]		*[[PE/PPE Protein Complex\|PE/PPE Protein Complex]]
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Myctu~~]]	+	[[Category: Mycobacterium tuberculosis H37Rv]]
-	[[Category: Eisenberg, D]]	+	[[Category: Eisenberg D]]
-	[[Category: Sawaya~~, M R~~]]	+	[[Category: Sawaya MR]]
-	[[Category: Strong, M]]	+	[[Category: Strong M]]
-	~~[[Category: Structural genomic]]~~	+
-	~~[[Category: PSI, Protein structure initiative]]~~	+
-	~~[[Category: Protein-protein complex]]~~	+
-	~~[[Category: Tbsgc]]~~	+
-	~~[[Category: Unknown function~~]]	+

OCA at 15:39, 3 March 2021

OCA — Wed, 03 Mar 2021 15:39:03 GMT

←Older revision		Revision as of 15:39, 3 March 2021
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	==A PE/PPE Protein Complex from Mycobacterium tuberculosis==		==A PE/PPE Protein Complex from Mycobacterium tuberculosis==
-	<StructureSection load='2g38' size='340' side='right' caption='[[2g38]], [[Resolution\|resolution]] 2.20Å' scene=''>	+	<StructureSection load='2g38' size='340' side='right'caption='[[2g38]], [[Resolution\|resolution]] 2.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2g38]] is a 4 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Myctu Myctu]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2G38 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2G38 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2g38]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Myctu Myctu]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2G38 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2G38 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Rv2431c ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83332 MYCTU]), Rv2430c ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83332 MYCTU])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Rv2431c ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83332 MYCTU]), Rv2430c ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83332 MYCTU])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2g38 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2g38 OCA], [~~http~~://pdbe.org/2g38 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2g38 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2g38 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2g38 ProSAT], [~~http~~://www.topsan.org/Proteins/ISFI/2g38 TOPSAN]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2g38 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2g38 OCA], [https://pdbe.org/2g38 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2g38 RCSB], [https://www.ebi.ac.uk/pdbsum/2g38 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2g38 ProSAT], [https://www.topsan.org/Proteins/ISFI/2g38 TOPSAN]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 12:		Line 12:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/g3/2g38_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/g3/2g38_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
Line 27:		Line 27:
	</div>		</div>
	<div class="pdbe-citations 2g38" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 2g38" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[PE/PPE Protein Complex\|PE/PPE Protein Complex]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Myctu]]		[[Category: Myctu]]
	[[Category: Eisenberg, D]]		[[Category: Eisenberg, D]]

OCA at 07:47, 18 October 2017

OCA — Wed, 18 Oct 2017 07:47:44 GMT

←Older revision		Revision as of 07:47, 18 October 2017
Line 1:		Line 1:
		+
	==A PE/PPE Protein Complex from Mycobacterium tuberculosis==		==A PE/PPE Protein Complex from Mycobacterium tuberculosis==
	<StructureSection load='2g38' size='340' side='right' caption='[[2g38]], [[Resolution\|resolution]] 2.20Å' scene=''>		<StructureSection load='2g38' size='340' side='right' caption='[[2g38]], [[Resolution\|resolution]] 2.20Å' scene=''>
Line 5:		Line 6:
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Rv2431c ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83332 MYCTU]), Rv2430c ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83332 MYCTU])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Rv2431c ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83332 MYCTU]), Rv2430c ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83332 MYCTU])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2g38 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2g38 OCA], [http://pdbe.org/2g38 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2g38 RCSB], [http://www.ebi.ac.uk/pdbsum/2g38 PDBsum], [http://www.topsan.org/Proteins/ISFI/2g38 TOPSAN]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2g38 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2g38 OCA], [http://pdbe.org/2g38 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2g38 RCSB], [http://www.ebi.ac.uk/pdbsum/2g38 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2g38 ProSAT], [http://www.topsan.org/Proteins/ISFI/2g38 TOPSAN]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
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	</div>		</div>
	<div class="pdbe-citations 2g38" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 2g38" style="background-color:#fffaf0;"></div>
-
-	==See Also==
-	*[[PE/PPE Protein Complex\|PE/PPE Protein Complex]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 03:18, 10 February 2016

OCA — Wed, 10 Feb 2016 03:18:09 GMT

←Older revision		Revision as of 03:18, 10 February 2016
Line 15:		Line 15:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2g38 ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 04:50, 11 September 2015

OCA — Fri, 11 Sep 2015 04:50:58 GMT

←Older revision		Revision as of 04:50, 11 September 2015
Line 5:		Line 5:
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Rv2431c ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83332 MYCTU]), Rv2430c ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83332 MYCTU])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Rv2431c ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83332 MYCTU]), Rv2430c ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83332 MYCTU])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2g38 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2g38 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2g38 RCSB], [http://www.ebi.ac.uk/pdbsum/2g38 PDBsum], [http://www.topsan.org/Proteins/ISFI/2g38 TOPSAN]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2g38 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2g38 OCA], [http://pdbe.org/2g38 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2g38 RCSB], [http://www.ebi.ac.uk/pdbsum/2g38 PDBsum], [http://www.topsan.org/Proteins/ISFI/2g38 TOPSAN]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 25:		Line 25:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 2g38" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==

OCA at 11:25, 25 December 2014

OCA — Thu, 25 Dec 2014 11:25:47 GMT

←Older revision		Revision as of 11:25, 25 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2g38]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Myctu Myctu]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2G38 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2G38 FirstGlance]. <br>		<table><tr><td colspan='2'>[[2g38]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Myctu Myctu]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2G38 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2G38 FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Rv2431c ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83332 MYCTU]), Rv2430c ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83332 MYCTU])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Rv2431c ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83332 MYCTU]), Rv2430c ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83332 MYCTU])</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2g38 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2g38 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2g38 RCSB], [http://www.ebi.ac.uk/pdbsum/2g38 PDBsum], [http://www.topsan.org/Proteins/ISFI/2g38 TOPSAN]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2g38 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2g38 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2g38 RCSB], [http://www.ebi.ac.uk/pdbsum/2g38 PDBsum], [http://www.topsan.org/Proteins/ISFI/2g38 TOPSAN]</span></td></tr>
-	<table>	+	</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 33:		Line 33:
	</StructureSection>		</StructureSection>
	[[Category: Myctu]]		[[Category: Myctu]]
-	[[Category: Eisenberg, D.]]	+	[[Category: Eisenberg, D]]
-	[[Category: Sawaya, M R.]]	+	[[Category: Sawaya, M R]]
-	[[Category: Strong, M~~.]]~~	+	[[Category: Strong, M]]
-	~~[[Category: TBSGC, TB Structural Genomics Consortium.]]~~	+
-	~~[[Category: Protein structure initiative]]~~	+
-	~~[[Category: Protein-protein complex]]~~	+
-	~~[[Category: Psi~~]]	+
	[[Category: Structural genomic]]		[[Category: Structural genomic]]
-	[[Category: ~~Tb structural genomics consortium~~]]	+	[[Category: PSI, Protein structure initiative]]
		+	[[Category: Protein-protein complex]]
	[[Category: Tbsgc]]		[[Category: Tbsgc]]
	[[Category: Unknown function]]		[[Category: Unknown function]]

OCA at 08:34, 3 October 2014

OCA — Fri, 03 Oct 2014 08:34:40 GMT

←Older revision		Revision as of 08:34, 3 October 2014
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-	~~{{STRUCTURE_2g38\| PDB=2g38 \| SCENE= }}~~	+	==A PE/PPE Protein Complex from Mycobacterium tuberculosis==
-	===A PE/PPE Protein Complex from Mycobacterium tuberculosis===	+	<StructureSection load='2g38' size='340' side='right' caption='[[2g38]], [[Resolution\|resolution]] 2.20Å' scene=''>
-	~~{{ABSTRACT_PUBMED_16690741}}~~	+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[2g38]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Myctu Myctu]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2G38 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2G38 FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Rv2431c ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83332 MYCTU]), Rv2430c ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83332 MYCTU])</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2g38 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2g38 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2g38 RCSB], [http://www.ebi.ac.uk/pdbsum/2g38 PDBsum], [http://www.topsan.org/Proteins/ISFI/2g38 TOPSAN]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/g3/2g38_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The developing science called structural genomics has focused to date mainly on high-throughput expression of individual proteins, followed by their purification and structure determination. In contrast, the term structural biology is used to denote the determination of structures, often complexes of several macromolecules, that illuminate aspects of biological function. Here we bridge structural genomics to structural biology with a procedure for determining protein complexes of previously unknown function from any organism with a sequenced genome. From computational genomic analysis, we identify functionally linked proteins and verify their interaction in vitro by coexpression/copurification. We illustrate this procedure by the structural determination of a previously unknown complex between a PE and PPE protein from the Mycobacterium tuberculosis genome, members of protein families that constitute approximately 10% of the coding capacity of this genome. The predicted complex was readily expressed, purified, and crystallized, although we had previously failed in expressing individual PE and PPE proteins on their own. The reason for the failure is clear from the structure, which shows that the PE and PPE proteins mate along an extended apolar interface to form a four-alpha-helical bundle, where two of the alpha-helices are contributed by the PE protein and two by the PPE protein. Our entire procedure for the identification, characterization, and structural determination of protein complexes can be scaled to a genome-wide level.

-	~~==About this Structure==~~	+	Toward the structural genomics of complexes: crystal structure of a PE/PPE protein complex from Mycobacterium tuberculosis.,Strong M, Sawaya MR, Wang S, Phillips M, Cascio D, Eisenberg D Proc Natl Acad Sci U S A. 2006 May 23;103(21):8060-5. Epub 2006 May 11. PMID:16690741<ref>PMID:16690741</ref>
-	~~[[2g38]] is a 4 chain structure with sequence from [http~~:/~~/en~~.~~wikipedia~~.~~org/wiki/Myctu Myctu]~~. ~~Full crystallographic information is available from [http~~://~~oca~~.~~weizmann~~.ac.il/~~oca-bin/ocashort?id=2G38 OCA].~~	+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>

	==See Also==		==See Also==
	*[[PE/PPE Protein Complex\|PE/PPE Protein Complex]]		*[[PE/PPE Protein Complex\|PE/PPE Protein Complex]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	~~<ref group="xtra">PMID:016690741</ref>~~<references ~~group="xtra"~~/><~~references~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Myctu]]		[[Category: Myctu]]
	[[Category: Eisenberg, D.]]		[[Category: Eisenberg, D.]]

OCA at 08:18, 23 April 2014

OCA — Wed, 23 Apr 2014 08:18:47 GMT

←Older revision		Revision as of 08:18, 23 April 2014
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-	[[Image:2g38.png\|left\|200px]]
-
-	<!--
-	The line below this paragraph, containing "STRUCTURE_2g38", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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	{{STRUCTURE_2g38\| PDB=2g38 \| SCENE= }}		{{STRUCTURE_2g38\| PDB=2g38 \| SCENE= }}
-
	===A PE/PPE Protein Complex from Mycobacterium tuberculosis===		===A PE/PPE Protein Complex from Mycobacterium tuberculosis===
-
-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_16690741}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 16690741 is the PubMed ID number.
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	{{ABSTRACT_PUBMED_16690741}}		{{ABSTRACT_PUBMED_16690741}}

	==About this Structure==		==About this Structure==
-	[[2g38]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Mycobacterium_tuberculosis Mycobacterium tuberculosis~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2G38 OCA].	+	[[2g38]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Myctu Myctu]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2G38 OCA].

	==See Also==		==See Also==
-	*[[~~User:Michael Strong~~\|~~User:Michael Strong~~]]	+	*[[PE/PPE Protein Complex\|PE/PPE Protein Complex]]

	==Reference==		==Reference==
-	<ref group="xtra">PMID:016690741</ref><references group="xtra"/>	+	<ref group="xtra">PMID:016690741</ref><references group="xtra"/><references/>
-	[[Category: ~~Mycobacterium tuberculosis~~]]	+	[[Category: Myctu]]
	[[Category: Eisenberg, D.]]		[[Category: Eisenberg, D.]]
	[[Category: Sawaya, M R.]]		[[Category: Sawaya, M R.]]

OCA: Protected "2g38" [edit=sysop:move=sysop]

OCA — Sun, 10 Jun 2012 15:55:13 GMT

Protected "2g38" [edit=sysop:move=sysop]

←Older revision

Revision as of 15:55, 10 June 2012