2g3p - Revision history

OCA at 15:44, 4 October 2023

OCA — Wed, 04 Oct 2023 15:44:35 GMT

←Older revision		Revision as of 15:44, 4 October 2023
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	<StructureSection load='2g3p' size='340' side='right'caption='[[2g3p]], [[Resolution\|resolution]] 1.90Å' scene=''>		<StructureSection load='2g3p' size='340' side='right'caption='[[2g3p]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2g3p]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Bpfd Bpfd~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2G3P OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2G3P FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2g3p]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Enterobacteria_phage_fd Enterobacteria phage fd]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2G3P OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2G3P FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2g3p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2g3p OCA], [https://pdbe.org/2g3p PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2g3p RCSB], [https://www.ebi.ac.uk/pdbsum/2g3p PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2g3p ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.9Å</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2g3p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2g3p OCA], [https://pdbe.org/2g3p PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2g3p RCSB], [https://www.ebi.ac.uk/pdbsum/2g3p PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2g3p ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/G3P_BPFD G3P_BPFD]] Plays essential roles both in the penetration of the viral genome into the bacterial host via pilus retraction and in the extrusion process. During the initial step of infection, G3P mediates adsorption of the phage to its primary receptor, the tip of host F-pilus. Subsequent interaction with the host entry receptor tolA induces penetration of the viral DNA into the host cytoplasm. In the extrusion process, G3P mediates the release of the membrane-anchored virion from the cell via its C-terminal domain.<ref>PMID:12054858</ref> <ref>PMID:21110981</ref>	+	[https://www.uniprot.org/uniprot/G3P_BPFD G3P_BPFD] Plays essential roles both in the penetration of the viral genome into the bacterial host via pilus retraction and in the extrusion process. During the initial step of infection, G3P mediates adsorption of the phage to its primary receptor, the tip of host F-pilus. Subsequent interaction with the host entry receptor tolA induces penetration of the viral DNA into the host cytoplasm. In the extrusion process, G3P mediates the release of the membrane-anchored virion from the cell via its C-terminal domain.<ref>PMID:12054858</ref> <ref>PMID:21110981</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 24:		Line 25:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Bpfd~~]]	+	[[Category: Enterobacteria phage fd]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Holliger, P]]	+	[[Category: Holliger P]]
-	[[Category: Williams~~, R L]]~~	+	[[Category: Williams RL]]
-	~~[[Category: Beta barrel]]~~	+
-	~~[[Category: Conformational change]]~~	+
-	~~[[Category: Filamentous phage]]~~	+
-	~~[[Category: Infection]]~~	+
-	~~[[Category: Pilus binding]]~~	+
-	~~[[Category: Viral protein~~]]	+

OCA at 14:39, 29 December 2021

OCA — Wed, 29 Dec 2021 14:39:45 GMT

←Older revision		Revision as of 14:39, 29 December 2021
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	<StructureSection load='2g3p' size='340' side='right'caption='[[2g3p]], [[Resolution\|resolution]] 1.90Å' scene=''>		<StructureSection load='2g3p' size='340' side='right'caption='[[2g3p]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2g3p]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Bpfd Bpfd]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2G3P OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2G3P FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2g3p]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bpfd Bpfd]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2G3P OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2G3P FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2g3p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2g3p OCA], [~~http~~://pdbe.org/2g3p PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2g3p RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2g3p PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2g3p ProSAT]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2g3p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2g3p OCA], [https://pdbe.org/2g3p PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2g3p RCSB], [https://www.ebi.ac.uk/pdbsum/2g3p PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2g3p ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/G3P_BPFD G3P_BPFD]] Plays essential roles both in the penetration of the viral genome into the bacterial host via pilus retraction and in the extrusion process. During the initial step of infection, G3P mediates adsorption of the phage to its primary receptor, the tip of host F-pilus. Subsequent interaction with the host entry receptor tolA induces penetration of the viral DNA into the host cytoplasm. In the extrusion process, G3P mediates the release of the membrane-anchored virion from the cell via its C-terminal domain.<ref>PMID:12054858</ref> <ref>PMID:21110981</ref>	+	[[https://www.uniprot.org/uniprot/G3P_BPFD G3P_BPFD]] Plays essential roles both in the penetration of the viral genome into the bacterial host via pilus retraction and in the extrusion process. During the initial step of infection, G3P mediates adsorption of the phage to its primary receptor, the tip of host F-pilus. Subsequent interaction with the host entry receptor tolA induces penetration of the viral DNA into the host cytoplasm. In the extrusion process, G3P mediates the release of the membrane-anchored virion from the cell via its C-terminal domain.<ref>PMID:12054858</ref> <ref>PMID:21110981</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==

OCA at 11:08, 27 March 2020

OCA — Fri, 27 Mar 2020 11:08:38 GMT

←Older revision		Revision as of 11:08, 27 March 2020
Line 1:		Line 1:

	==STRUCTURE OF THE N-TERMINAL TWO DOMAINS OF THE INFECTIVITY PROTEIN G3P OF FILAMENTOUS PHAGE FD==		==STRUCTURE OF THE N-TERMINAL TWO DOMAINS OF THE INFECTIVITY PROTEIN G3P OF FILAMENTOUS PHAGE FD==
-	<StructureSection load='2g3p' size='340' side='right' caption='[[2g3p]], [[Resolution\|resolution]] 1.90Å' scene=''>	+	<StructureSection load='2g3p' size='340' side='right'caption='[[2g3p]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2g3p]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bpfd Bpfd]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2G3P OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2G3P FirstGlance]. <br>		<table><tr><td colspan='2'>[[2g3p]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bpfd Bpfd]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2G3P OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2G3P FirstGlance]. <br>
Line 25:		Line 25:
	</StructureSection>		</StructureSection>
	[[Category: Bpfd]]		[[Category: Bpfd]]
		+	[[Category: Large Structures]]
	[[Category: Holliger, P]]		[[Category: Holliger, P]]
	[[Category: Williams, R L]]		[[Category: Williams, R L]]

OCA at 07:05, 20 June 2018

OCA — Wed, 20 Jun 2018 07:05:30 GMT

←Older revision		Revision as of 07:05, 20 June 2018
Line 1:		Line 1:
		+
	==STRUCTURE OF THE N-TERMINAL TWO DOMAINS OF THE INFECTIVITY PROTEIN G3P OF FILAMENTOUS PHAGE FD==		==STRUCTURE OF THE N-TERMINAL TWO DOMAINS OF THE INFECTIVITY PROTEIN G3P OF FILAMENTOUS PHAGE FD==
	<StructureSection load='2g3p' size='340' side='right' caption='[[2g3p]], [[Resolution\|resolution]] 1.90Å' scene=''>		<StructureSection load='2g3p' size='340' side='right' caption='[[2g3p]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2g3p]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bpfd Bpfd]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2G3P OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2G3P FirstGlance]. <br>		<table><tr><td colspan='2'>[[2g3p]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bpfd Bpfd]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2G3P OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2G3P FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2g3p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2g3p OCA], [http://pdbe.org/2g3p PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2g3p RCSB], [http://www.ebi.ac.uk/pdbsum/2g3p PDBsum]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2g3p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2g3p OCA], [http://pdbe.org/2g3p PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2g3p RCSB], [http://www.ebi.ac.uk/pdbsum/2g3p PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2g3p ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==

OCA at 08:32, 11 September 2015

OCA — Fri, 11 Sep 2015 08:32:57 GMT

←Older revision		Revision as of 08:32, 11 September 2015
Line 2:		Line 2:
	<StructureSection load='2g3p' size='340' side='right' caption='[[2g3p]], [[Resolution\|resolution]] 1.90Å' scene=''>		<StructureSection load='2g3p' size='340' side='right' caption='[[2g3p]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2g3p]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Enterobacteria_phage_fd Enterobacteria phage fd~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2G3P OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2G3P FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2g3p]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bpfd Bpfd]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2G3P OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2G3P FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2g3p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2g3p OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2g3p RCSB], [http://www.ebi.ac.uk/pdbsum/2g3p PDBsum]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2g3p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2g3p OCA], [http://pdbe.org/2g3p PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2g3p RCSB], [http://www.ebi.ac.uk/pdbsum/2g3p PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[http://www.uniprot.org/uniprot/~~COATA_BPFD COATA_BPFD~~]] Plays essential roles both in the penetration of the viral genome into the bacterial host via pilus retraction and in the extrusion process. During the initial step of infection, G3P mediates adsorption of the phage to its primary receptor, the tip of host F-pilus. Subsequent interaction with the host entry receptor tolA induces penetration of the viral DNA into the host cytoplasm. In the extrusion process, G3P mediates the release of the membrane-anchored virion from the cell via its C-terminal domain.<ref>PMID:12054858</ref> <ref>PMID:21110981</ref>	+	[[http://www.uniprot.org/uniprot/G3P_BPFD G3P_BPFD]] Plays essential roles both in the penetration of the viral genome into the bacterial host via pilus retraction and in the extrusion process. During the initial step of infection, G3P mediates adsorption of the phage to its primary receptor, the tip of host F-pilus. Subsequent interaction with the host entry receptor tolA induces penetration of the viral DNA into the host cytoplasm. In the extrusion process, G3P mediates the release of the membrane-anchored virion from the cell via its C-terminal domain.<ref>PMID:12054858</ref> <ref>PMID:21110981</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 15:		Line 15:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 2g3p" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 22:		Line 23:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Enterobacteria phage fd~~]]	+	[[Category: Bpfd]]
	[[Category: Holliger, P]]		[[Category: Holliger, P]]
	[[Category: Williams, R L]]		[[Category: Williams, R L]]

OCA at 13:55, 25 December 2014

OCA — Thu, 25 Dec 2014 13:55:12 GMT

←Older revision		Revision as of 13:55, 25 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2g3p]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Enterobacteria_phage_fd Enterobacteria phage fd]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2G3P OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2G3P FirstGlance]. <br>		<table><tr><td colspan='2'>[[2g3p]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Enterobacteria_phage_fd Enterobacteria phage fd]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2G3P OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2G3P FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2g3p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2g3p OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2g3p RCSB], [http://www.ebi.ac.uk/pdbsum/2g3p PDBsum]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2g3p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2g3p OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2g3p RCSB], [http://www.ebi.ac.uk/pdbsum/2g3p PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/COATA_BPFD COATA_BPFD]] Plays essential roles both in the penetration of the viral genome into the bacterial host via pilus retraction and in the extrusion process. During the initial step of infection, G3P mediates adsorption of the phage to its primary receptor, the tip of host F-pilus. Subsequent interaction with the host entry receptor tolA induces penetration of the viral DNA into the host cytoplasm. In the extrusion process, G3P mediates the release of the membrane-anchored virion from the cell via its C-terminal domain.<ref>PMID:12054858</ref> <ref>PMID:21110981</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 21:		Line 23:
	</StructureSection>		</StructureSection>
	[[Category: Enterobacteria phage fd]]		[[Category: Enterobacteria phage fd]]
-	[[Category: Holliger, P.]]	+	[[Category: Holliger, P]]
-	[[Category: Williams, R L.]]	+	[[Category: Williams, R L]]
	[[Category: Beta barrel]]		[[Category: Beta barrel]]
	[[Category: Conformational change]]		[[Category: Conformational change]]

OCA at 05:11, 29 September 2014

OCA — Mon, 29 Sep 2014 05:11:38 GMT

←Older revision		Revision as of 05:11, 29 September 2014
Line 1:		Line 1:
-	[[~~Image~~:2g3p.~~png\|left\|200px~~]]	+	==STRUCTURE OF THE N-TERMINAL TWO DOMAINS OF THE INFECTIVITY PROTEIN G3P OF FILAMENTOUS PHAGE FD==
		+	<StructureSection load='2g3p' size='340' side='right' caption='[[2g3p]], [[Resolution\|resolution]] 1.90Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[2g3p]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Enterobacteria_phage_fd Enterobacteria phage fd]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2G3P OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2G3P FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2g3p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2g3p OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2g3p RCSB], [http://www.ebi.ac.uk/pdbsum/2g3p PDBsum]</span></td></tr>
		+	<table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Infection of Escherichia coli by filamentous bacteriophages is mediated by the minor phage coat protein g3p and involves two distinct cellular receptors, the F' pilus and the periplasmic protein TolA. Recently we have shown that the two receptors are contacted in a sequential manner, such that binding of TolA by the N-terminal domain g3p-D1 is conditional on a primary interaction of the second g3p domain D2 with the F' pilus. In order to better understand this process, we have solved the crystal structure of the g3p-D1D2 fragment (residues 2-217) from filamentous phage fd to 1.9 A resolution and compared it to the recently published structure of the same fragment from the related Ff phage M13. While the structure of individual domains D1 and D2 of the two phages are very similar (rms<0.7 A), there is comparatively poor agreement for the overall D1D2 structure (rms>1.2 A). This is due to an apparent movement of domain D2 with respect to D1, which results in a widening of the inter-domain groove compared to the structure of the homologous M13 protein. The movement of D2 can be described as a rigid-body rotation around a hinge located at the end of a short anti-parallel beta-sheet connecting domains D1 and D2. Structural flexibility of at least parts of the D1D2 structure was also suggested by studying the thermal unfolding of g3p: the TolA binding site on D1, while fully blocked by D2 at 37 degrees C, becomes accessible after incubation at temperatures as low as 45 degrees C. Our results support a model for the early steps of phage infection whereby exposure of the coreceptor binding site on D1 is facilitated by a conformational change in the D1D2 structure, which in vivo is induced by binding to the F' pilus on the host cell and which can be mimicked in vitro by thermal unfolding.

-	~~{{STRUCTURE_2g3p\| PDB=2g3p \| SCENE= }}~~	+	Crystal structure of the two N-terminal domains of g3p from filamentous phage fd at 1.9 A: evidence for conformational lability.,Holliger P, Riechmann L, Williams RL J Mol Biol. 1999 May 14;288(4):649-57. PMID:10329170<ref>PMID:10329170</ref>

-	~~===STRUCTURE OF THE N-TERMINAL TWO DOMAINS OF THE INFECTIVITY PROTEIN G3P OF FILAMENTOUS PHAGE FD===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_10329170}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[2g3p]] is~~ a ~~2 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Enterobacteria_phage_fd Enterobacteria phage fd]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=2G3P OCA].~~	+

	==See Also==		==See Also==
	*[[G3p\|G3p]]		*[[G3p\|G3p]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	<~~ref group="xtra">PMID:010329170<~~/~~ref~~><~~references group="xtra"~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Enterobacteria phage fd]]		[[Category: Enterobacteria phage fd]]
	[[Category: Holliger, P.]]		[[Category: Holliger, P.]]

OCA at 17:30, 20 October 2012

OCA — Sat, 20 Oct 2012 17:30:04 GMT

←Older revision		Revision as of 17:30, 20 October 2012
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	==About this Structure==		==About this Structure==
-	[[2g3p]] is a 2 chain structure ~~of [[G3p]]~~ with sequence from [http://en.wikipedia.org/wiki/Enterobacteria_phage_fd Enterobacteria phage fd]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2G3P OCA].	+	[[2g3p]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Enterobacteria_phage_fd Enterobacteria phage fd]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2G3P OCA].

	==See Also==		==See Also==

OCA: Protected "2g3p" [edit=sysop:move=sysop]

OCA — Fri, 27 Jul 2012 01:05:23 GMT

Protected "2g3p" [edit=sysop:move=sysop]

←Older revision

Revision as of 01:05, 27 July 2012

OCA at 01:05, 27 July 2012

OCA — Fri, 27 Jul 2012 01:05:22 GMT

←Older revision		Revision as of 01:05, 27 July 2012
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-	{{Seed}}
	[[Image:2g3p.png\|left\|200px]]		[[Image:2g3p.png\|left\|200px]]

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	{{STRUCTURE_2g3p\| PDB=2g3p \| SCENE= }}		{{STRUCTURE_2g3p\| PDB=2g3p \| SCENE= }}

	===STRUCTURE OF THE N-TERMINAL TWO DOMAINS OF THE INFECTIVITY PROTEIN G3P OF FILAMENTOUS PHAGE FD===		===STRUCTURE OF THE N-TERMINAL TWO DOMAINS OF THE INFECTIVITY PROTEIN G3P OF FILAMENTOUS PHAGE FD===

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	{{ABSTRACT_PUBMED_10329170}}		{{ABSTRACT_PUBMED_10329170}}

	==About this Structure==		==About this Structure==
-	~~2G3P is a~~ [[~~Single protein~~]] structure of sequence from [http://en.wikipedia.org/wiki/Enterobacteria_phage_fd Enterobacteria phage fd]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2G3P OCA].	+	[[2g3p]] is a 2 chain structure of [[G3p]] with sequence from [http://en.wikipedia.org/wiki/Enterobacteria_phage_fd Enterobacteria phage fd]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2G3P OCA].
		+
		+	==See Also==
		+	*[[G3p\|G3p]]

	==Reference==		==Reference==
-	Crystal structure of the two N-terminal domains of g3p from filamentous phage fd at 1.9 A: evidence for conformational lability., Holliger P, Riechmann L, Williams RL, J Mol Biol. 1999 May 14;288(4):649-57. PMID:~~[http://www.ncbi.nlm.nih.gov~~/~~pubmed~~/~~10329170 10329170]~~	+	<ref group="xtra">PMID:010329170</ref><references group="xtra"/>
	[[Category: Enterobacteria phage fd]]		[[Category: Enterobacteria phage fd]]
-	[[Category: Single protein]]
	[[Category: Holliger, P.]]		[[Category: Holliger, P.]]
	[[Category: Williams, R L.]]		[[Category: Williams, R L.]]
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	[[Category: Infection]]		[[Category: Infection]]
	[[Category: Pilus binding]]		[[Category: Pilus binding]]
-		+	[[Category: Viral protein]]
-	~~''Page seeded by~~ [~~http~~:~~//oca.weizmann.ac.il/oca OCA~~ ] ~~on Tue Jul 29 01:15:10 2008''~~	+