2g8c - Revision history

OCA at 09:36, 30 August 2023

OCA — Wed, 30 Aug 2023 09:36:59 GMT

←Older revision		Revision as of 09:36, 30 August 2023
Line 3:		Line 3:
	<StructureSection load='2g8c' size='340' side='right'caption='[[2g8c]], [[Resolution\|resolution]] 1.15Å' scene=''>		<StructureSection load='2g8c' size='340' side='right'caption='[[2g8c]], [[Resolution\|resolution]] 1.15Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2g8c]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Atcc_35210 Atcc 35210~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2G8C OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2G8C FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2g8c]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Borreliella_burgdorferi Borreliella burgdorferi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2G8C OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2G8C FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=IMD:IMIDAZOLE'>IMD</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.15Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=IMD:IMIDAZOLE'>IMD</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2g8c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2g8c OCA], [https://pdbe.org/2g8c PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2g8c RCSB], [https://www.ebi.ac.uk/pdbsum/2g8c PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2g8c ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2g8c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2g8c OCA], [https://pdbe.org/2g8c PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2g8c RCSB], [https://www.ebi.ac.uk/pdbsum/2g8c PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2g8c ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/OSPA_BORBU OSPA_BORBU]
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Atcc 35210~~]]	+	[[Category: Borreliella burgdorferi]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Koide, S]]	+	[[Category: Koide S]]
-	[[Category: Makabe, K]]	+	[[Category: Makabe K]]
-	[[Category: Tereshko, V]]	+	[[Category: Tereshko V]]
-	~~[[Category: Beta sheet]]~~	+
-	~~[[Category: Lipid binding protein~~]]	+

OCA at 17:30, 20 October 2021

OCA — Wed, 20 Oct 2021 17:30:55 GMT

←Older revision		Revision as of 17:30, 20 October 2021
Line 3:		Line 3:
	<StructureSection load='2g8c' size='340' side='right'caption='[[2g8c]], [[Resolution\|resolution]] 1.15Å' scene=''>		<StructureSection load='2g8c' size='340' side='right'caption='[[2g8c]], [[Resolution\|resolution]] 1.15Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2g8c]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Atcc_35210 Atcc 35210]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2G8C OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2G8C FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2g8c]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Atcc_35210 Atcc 35210]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2G8C OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2G8C FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=IMD:IMIDAZOLE'>IMD</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=IMD:IMIDAZOLE'>IMD</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2g8c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2g8c OCA], [~~http~~://pdbe.org/2g8c PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2g8c RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2g8c PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2g8c ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2g8c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2g8c OCA], [https://pdbe.org/2g8c PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2g8c RCSB], [https://www.ebi.ac.uk/pdbsum/2g8c PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2g8c ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==

OCA at 11:10, 27 March 2020

OCA — Fri, 27 Mar 2020 11:10:50 GMT

←Older revision		Revision as of 11:10, 27 March 2020
Line 1:		Line 1:

	==Atomic-resolution crystal structure of Borrelia burgdorferi OspA via surface entropy reduction==		==Atomic-resolution crystal structure of Borrelia burgdorferi OspA via surface entropy reduction==
-	<StructureSection load='2g8c' size='340' side='right' caption='[[2g8c]], [[Resolution\|resolution]] 1.15Å' scene=''>	+	<StructureSection load='2g8c' size='340' side='right'caption='[[2g8c]], [[Resolution\|resolution]] 1.15Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2g8c]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_35210 Atcc 35210]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2G8C OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2G8C FirstGlance]. <br>		<table><tr><td colspan='2'>[[2g8c]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_35210 Atcc 35210]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2G8C OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2G8C FirstGlance]. <br>
Line 34:		Line 34:
	</StructureSection>		</StructureSection>
	[[Category: Atcc 35210]]		[[Category: Atcc 35210]]
		+	[[Category: Large Structures]]
	[[Category: Koide, S]]		[[Category: Koide, S]]
	[[Category: Makabe, K]]		[[Category: Makabe, K]]

OCA at 06:59, 20 June 2018

OCA — Wed, 20 Jun 2018 06:59:22 GMT

←Older revision		Revision as of 06:59, 20 June 2018
Line 1:		Line 1:
		+
	==Atomic-resolution crystal structure of Borrelia burgdorferi OspA via surface entropy reduction==		==Atomic-resolution crystal structure of Borrelia burgdorferi OspA via surface entropy reduction==
	<StructureSection load='2g8c' size='340' side='right' caption='[[2g8c]], [[Resolution\|resolution]] 1.15Å' scene=''>		<StructureSection load='2g8c' size='340' side='right' caption='[[2g8c]], [[Resolution\|resolution]] 1.15Å' scene=''>
Line 4:		Line 5:
	<table><tr><td colspan='2'>[[2g8c]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_35210 Atcc 35210]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2G8C OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2G8C FirstGlance]. <br>		<table><tr><td colspan='2'>[[2g8c]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_35210 Atcc 35210]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2G8C OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2G8C FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=IMD:IMIDAZOLE'>IMD</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=IMD:IMIDAZOLE'>IMD</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2g8c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2g8c OCA], [http://pdbe.org/2g8c PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2g8c RCSB], [http://www.ebi.ac.uk/pdbsum/2g8c PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2g8c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2g8c OCA], [http://pdbe.org/2g8c PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2g8c RCSB], [http://www.ebi.ac.uk/pdbsum/2g8c PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2g8c ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 10:		Line 11:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/g8/2g8c_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/g8/2g8c_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>

OCA at 07:44, 9 February 2016

OCA — Tue, 09 Feb 2016 07:44:22 GMT

←Older revision		Revision as of 07:44, 9 February 2016
Line 14:		Line 14:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2g8c ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 20:05, 10 September 2015

OCA — Thu, 10 Sep 2015 20:05:52 GMT

←Older revision		Revision as of 20:05, 10 September 2015
Line 2:		Line 2:
	<StructureSection load='2g8c' size='340' side='right' caption='[[2g8c]], [[Resolution\|resolution]] 1.15Å' scene=''>		<StructureSection load='2g8c' size='340' side='right' caption='[[2g8c]], [[Resolution\|resolution]] 1.15Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2g8c]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Borrelia_burgdorferi Borrelia burgdorferi~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2G8C OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2G8C FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2g8c]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_35210 Atcc 35210]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2G8C OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2G8C FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=IMD:IMIDAZOLE'>IMD</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=IMD:IMIDAZOLE'>IMD</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2g8c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2g8c OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2g8c RCSB], [http://www.ebi.ac.uk/pdbsum/2g8c PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2g8c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2g8c OCA], [http://pdbe.org/2g8c PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2g8c RCSB], [http://www.ebi.ac.uk/pdbsum/2g8c PDBsum]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 24:		Line 24:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 2g8c" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 31:		Line 32:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Borrelia burgdorferi~~]]	+	[[Category: Atcc 35210]]
	[[Category: Koide, S]]		[[Category: Koide, S]]
	[[Category: Makabe, K]]		[[Category: Makabe, K]]

OCA at 09:03, 16 January 2015

OCA — Fri, 16 Jan 2015 09:03:34 GMT

←Older revision		Revision as of 09:03, 16 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2g8c]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Borrelia_burgdorferi Borrelia burgdorferi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2G8C OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2G8C FirstGlance]. <br>		<table><tr><td colspan='2'>[[2g8c]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Borrelia_burgdorferi Borrelia burgdorferi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2G8C OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2G8C FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=IMD:IMIDAZOLE'>IMD</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=IMD:IMIDAZOLE'>IMD</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2g8c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2g8c OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2g8c RCSB], [http://www.ebi.ac.uk/pdbsum/2g8c PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2g8c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2g8c OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2g8c RCSB], [http://www.ebi.ac.uk/pdbsum/2g8c PDBsum]</span></td></tr>
-	<table>	+	</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 32:		Line 32:
	</StructureSection>		</StructureSection>
	[[Category: Borrelia burgdorferi]]		[[Category: Borrelia burgdorferi]]
-	[[Category: Koide, S.]]	+	[[Category: Koide, S]]
-	[[Category: Makabe, K.]]	+	[[Category: Makabe, K]]
-	[[Category: Tereshko, V.]]	+	[[Category: Tereshko, V]]
	[[Category: Beta sheet]]		[[Category: Beta sheet]]
	[[Category: Lipid binding protein]]		[[Category: Lipid binding protein]]

OCA at 05:07, 29 September 2014

OCA — Mon, 29 Sep 2014 05:07:28 GMT

←Older revision		Revision as of 05:07, 29 September 2014
Line 1:		Line 1:
-	[[~~Image~~:2g8c.~~png\|left~~\|200px]]	+	==Atomic-resolution crystal structure of Borrelia burgdorferi OspA via surface entropy reduction==
		+	<StructureSection load='2g8c' size='340' side='right' caption='[[2g8c]], [[Resolution\|resolution]] 1.15Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[2g8c]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Borrelia_burgdorferi Borrelia burgdorferi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2G8C OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2G8C FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=IMD:IMIDAZOLE'>IMD</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene><br>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2g8c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2g8c OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2g8c RCSB], [http://www.ebi.ac.uk/pdbsum/2g8c PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/g8/2g8c_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Outer surface protein A (OspA) from Borrelia burgdorferi has an unusual dumbbell-shaped structure in which two globular domains are connected with a "single-layer" beta-sheet (SLB). The protein is highly soluble, and it has been recalcitrant to crystallization. Only OspA complexes with Fab fragments have been successfully crystallized. OspA contains a large number of Lys and Glu residues, and these "high entropy" residues may disfavor crystal packing because some of them would need to be immobilized in forming a crystal lattice. We rationally designed a total of 13 surface mutations in which Lys and Glu residues were replaced with Ala or Ser. We successfully crystallized the mutant OspA without a bound Fab fragment and extended structure analysis to a 1.15 Angstroms resolution. The new high-resolution structure revealed a unique backbone hydration pattern of the SLB segment in which water molecules fill the "weak spots" on both faces of the antiparallel beta-sheet. These well-defined water molecules provide additional structural links between adjacent beta-strands, and thus they may be important for maintaining the rigidity of the SLB that inherently lacks tight packing afforded by a hydrophobic core. The structure also revealed new information on the side-chain dynamics and on a solvent-accessible cavity in the core of the C-terminal globular domain. This work demonstrates the utility of extensive surface mutation in crystallizing recalcitrant proteins and dramatically improving the resolution of crystal structures, and provides new insights into the stabilization mechanism of OspA.

-	~~{{STRUCTURE_2g8c\| PDB=2g8c \| SCENE= }}~~	+	Atomic-resolution crystal structure of Borrelia burgdorferi outer surface protein A via surface engineering.,Makabe K, Tereshko V, Gawlak G, Yan S, Koide S Protein Sci. 2006 Aug;15(8):1907-14. Epub 2006 Jul 5. PMID:16823038<ref>PMID:16823038</ref>

-	~~===Atomic-resolution crystal structure~~ of ~~Borrelia burgdorferi OspA via surface entropy reduction===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_16823038}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[2g8c]] is a 1 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Borrelia_burgdorferi Borrelia burgdorferi]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=2G8C OCA].~~	+

	==See Also==		==See Also==
	*[[Outer surface protein\|Outer surface protein]]		*[[Outer surface protein\|Outer surface protein]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	<~~ref group="xtra">PMID:016823038<~~/~~ref~~><~~references group="xtra"~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Borrelia burgdorferi]]		[[Category: Borrelia burgdorferi]]
	[[Category: Koide, S.]]		[[Category: Koide, S.]]

OCA: Protected "2g8c" [edit=sysop:move=sysop]

OCA — Sat, 20 Oct 2012 13:09:50 GMT

Protected "2g8c" [edit=sysop:move=sysop]

←Older revision

Revision as of 13:09, 20 October 2012

OCA at 13:09, 20 October 2012

OCA — Sat, 20 Oct 2012 13:09:48 GMT

←Older revision		Revision as of 13:09, 20 October 2012
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-	{{Seed}}
	[[Image:2g8c.png\|left\|200px]]		[[Image:2g8c.png\|left\|200px]]

-	<!--
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	{{STRUCTURE_2g8c\| PDB=2g8c \| SCENE= }}		{{STRUCTURE_2g8c\| PDB=2g8c \| SCENE= }}

	===Atomic-resolution crystal structure of Borrelia burgdorferi OspA via surface entropy reduction===		===Atomic-resolution crystal structure of Borrelia burgdorferi OspA via surface entropy reduction===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_16823038}}, adds the Publication Abstract to the page
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	{{ABSTRACT_PUBMED_16823038}}		{{ABSTRACT_PUBMED_16823038}}

	==About this Structure==		==About this Structure==
-	~~2G8C~~ is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Borrelia_burgdorferi Borrelia burgdorferi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2G8C OCA].	+	[[2g8c]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Borrelia_burgdorferi Borrelia burgdorferi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2G8C OCA].
		+
		+	==See Also==
		+	*[[Outer surface protein\|Outer surface protein]]

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~16823038~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:016823038</ref><references group="xtra"/>
	[[Category: Borrelia burgdorferi]]		[[Category: Borrelia burgdorferi]]
	[[Category: Koide, S.]]		[[Category: Koide, S.]]
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	[[Category: Tereshko, V.]]		[[Category: Tereshko, V.]]
	[[Category: Beta sheet]]		[[Category: Beta sheet]]
-		+	[[Category: Lipid binding protein]]
-	~~''Page seeded by~~ [~~http~~:~~//oca.weizmann.ac.il/oca OCA~~ ] ~~on Tue Feb 17 08:04:32 2009''~~	+