2gjn - Revision history

OCA at 13:51, 13 March 2024

OCA — Wed, 13 Mar 2024 13:51:58 GMT

←Older revision		Revision as of 13:51, 13 March 2024
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	<StructureSection load='2gjn' size='340' side='right'caption='[[2gjn]], [[Resolution\|resolution]] 2.30Å' scene=''>		<StructureSection load='2gjn' size='340' side='right'caption='[[2gjn]], [[Resolution\|resolution]] 2.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2gjn]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Pseae Pseae~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GJN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2GJN FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2gjn]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa_PAO1 Pseudomonas aeruginosa PAO1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GJN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2GJN FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene>~~, ~~<scene name='pdbligand=NIS:~~2~~-NITROPROPANE'>NIS</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.3Å</td></tr>
-	<tr id='~~activity~~'><td class="sblockLbl"><b>~~Activity~~:</b></td><td class="sblockDat"><~~span class~~='~~plainlinks~~'>~~[https:~~/~~/en.wikipedia.org/wiki/Nitronate_monooxygenase Nitronate monooxygenase]~~, ~~with EC number [https~~:~~//www.brenda~~-~~enzymes.info/php/result_flat.php4?ecno=1.13.12.16 1.13.12.16]~~ </~~span~~></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene>, <scene name='pdbligand=NIS:2-NITROPROPANE'>NIS</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2gjn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2gjn OCA], [https://pdbe.org/2gjn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2gjn RCSB], [https://www.ebi.ac.uk/pdbsum/2gjn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2gjn ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2gjn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2gjn OCA], [https://pdbe.org/2gjn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2gjn RCSB], [https://www.ebi.ac.uk/pdbsum/2gjn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2gjn ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/~~2NPD_PSEAE 2NPD_PSEAE]~~] Catalyzes the ~~oxidative denitrification~~ of ~~nitronates to their~~ corresponding ~~aldehydes~~ and ~~nitrites~~.<ref>PMID:~~16682407~~</ref>	+	[https://www.uniprot.org/uniprot/NQRED_PSEAE NQRED_PSEAE] Catalyzes the NADH-dependent reduction of a broad spectrum of quinone substrates, generating the corresponding hydroquinones. Highly prefers NADH to NADPH as a reducing substrate. Also displays a small NADH oxidase activity. Does not exhibit nitronate monooxygenase activity; is inactive against propionate 3-nitronate, 3-nitropropionate, nitroethane, 1-nitropropane, 2-nitropropane, and the anionic forms ethylnitronate, propyl-1-nitronate, and propyl-2-nitronate. Has no azoreductase activity since it is not able to reduce the azo dye methyl red with NADH. May be required to maintain an appropriate [NAD(+)]/[NADH] ratio for the catabolism of fatty acids in P.aeruginosa PAO1.<ref>PMID:27502282</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2gjn ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2gjn ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	Nitroalkane compounds are widely used in chemical industry and are also produced by microorganisms and plants. Some nitroalkanes have been demonstrated to be carcinogenic, and enzymatic oxidation of nitroalkanes is of considerable interest. 2-Nitropropane dioxygenases from Neurospora crassa and Williopsis mrakii (Hansenula mrakii), members of one family of the nitroalkane-oxidizing enzymes, contain FMN and FAD, respectively. The enzymatic oxidation of nitroalkanes by 2-nitropropane dioxygenase operates by an oxidase-style catalytic mechanism, which was recently shown to involve the formation of an anionic flavin semiquinone. This represents a unique case in which an anionic flavin semiquinone has been experimentally observed in the catalytic pathway for oxidation catalyzed by a flavin-dependent enzyme. Here we report the first crystal structure of 2-nitropropane dioxygenase from Pseudomonas aeruginosa in two forms: a binary complex with FMN and a ternary complex with both FMN and 2-nitropropane. The structure identifies His(152) as the proposed catalytic base, thus providing a structural framework for a better understanding of the catalytic mechanism.
-
-	Crystal structure of 2-nitropropane dioxygenase complexed with FMN and substrate. Identification of the catalytic base.,Ha JY, Min JY, Lee SK, Kim HS, Kim do J, Kim KH, Lee HH, Kim HK, Yoon HJ, Suh SW J Biol Chem. 2006 Jul 7;281(27):18660-7. Epub 2006 May 8. PMID:16682407<ref>PMID:16682407</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 2gjn" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
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	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Nitronate monooxygenase]]~~	+	[[Category: Pseudomonas aeruginosa PAO1]]
-	~~[[Category: Pseae~~]]	+	[[Category: Suh SW]]
-	[[Category: Suh~~, S W]]~~	+
-	~~[[Category: 2-nitropropane]]~~	+
-	~~[[Category: 2-nitropropane dioxygenase]]~~	+
-	~~[[Category: Fmn]]~~	+
-	~~[[Category: Oxidoreductase]]~~	+
-	~~[[Category: Substrate~~]]	+

OCA at 09:58, 5 May 2021

OCA — Wed, 05 May 2021 09:58:20 GMT

←Older revision		Revision as of 09:58, 5 May 2021
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	<StructureSection load='2gjn' size='340' side='right'caption='[[2gjn]], [[Resolution\|resolution]] 2.30Å' scene=''>		<StructureSection load='2gjn' size='340' side='right'caption='[[2gjn]], [[Resolution\|resolution]] 2.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2gjn]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Pseae Pseae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GJN OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2GJN FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2gjn]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseae Pseae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GJN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2GJN FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene>, <scene name='pdbligand=NIS:2-NITROPROPANE'>NIS</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene>, <scene name='pdbligand=NIS:2-NITROPROPANE'>NIS</scene></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Nitronate_monooxygenase Nitronate monooxygenase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.13.12.16 1.13.12.16] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Nitronate_monooxygenase Nitronate monooxygenase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.13.12.16 1.13.12.16] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2gjn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2gjn OCA], [~~http~~://pdbe.org/2gjn PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2gjn RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2gjn PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2gjn ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2gjn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2gjn OCA], [https://pdbe.org/2gjn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2gjn RCSB], [https://www.ebi.ac.uk/pdbsum/2gjn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2gjn ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/2NPD_PSEAE 2NPD_PSEAE]] Catalyzes the oxidative denitrification of nitronates to their corresponding aldehydes and nitrites.<ref>PMID:16682407</ref>	+	[[https://www.uniprot.org/uniprot/2NPD_PSEAE 2NPD_PSEAE]] Catalyzes the oxidative denitrification of nitronates to their corresponding aldehydes and nitrites.<ref>PMID:16682407</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 09:20, 24 July 2019

OCA — Wed, 24 Jul 2019 09:20:20 GMT

←Older revision		Revision as of 09:20, 24 July 2019
Line 1:		Line 1:

	==crystal structure of 2-nitropropane dioxygenase complexed with FMN and substrate==		==crystal structure of 2-nitropropane dioxygenase complexed with FMN and substrate==
-	<StructureSection load='2gjn' size='340' side='right' caption='[[2gjn]], [[Resolution\|resolution]] 2.30Å' scene=''>	+	<StructureSection load='2gjn' size='340' side='right'caption='[[2gjn]], [[Resolution\|resolution]] 2.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2gjn]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseae Pseae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GJN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2GJN FirstGlance]. <br>		<table><tr><td colspan='2'>[[2gjn]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseae Pseae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GJN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2GJN FirstGlance]. <br>
Line 14:		Line 14:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/gj/2gjn_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/gj/2gjn_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
Line 33:		Line 33:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Nitronate monooxygenase]]		[[Category: Nitronate monooxygenase]]
	[[Category: Pseae]]		[[Category: Pseae]]

OCA at 07:55, 18 October 2017

OCA — Wed, 18 Oct 2017 07:55:25 GMT

←Older revision		Revision as of 07:55, 18 October 2017
Line 1:		Line 1:
		+
	==crystal structure of 2-nitropropane dioxygenase complexed with FMN and substrate==		==crystal structure of 2-nitropropane dioxygenase complexed with FMN and substrate==
	<StructureSection load='2gjn' size='340' side='right' caption='[[2gjn]], [[Resolution\|resolution]] 2.30Å' scene=''>		<StructureSection load='2gjn' size='340' side='right' caption='[[2gjn]], [[Resolution\|resolution]] 2.30Å' scene=''>
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	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene>, <scene name='pdbligand=NIS:2-NITROPROPANE'>NIS</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene>, <scene name='pdbligand=NIS:2-NITROPROPANE'>NIS</scene></td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Nitronate_monooxygenase Nitronate monooxygenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.13.12.16 1.13.12.16] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Nitronate_monooxygenase Nitronate monooxygenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.13.12.16 1.13.12.16] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2gjn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2gjn OCA], [http://pdbe.org/2gjn PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2gjn RCSB], [http://www.ebi.ac.uk/pdbsum/2gjn PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2gjn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2gjn OCA], [http://pdbe.org/2gjn PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2gjn RCSB], [http://www.ebi.ac.uk/pdbsum/2gjn PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2gjn ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==

OCA at 09:42, 8 February 2016

OCA — Mon, 08 Feb 2016 09:42:37 GMT

←Older revision		Revision as of 09:42, 8 February 2016
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	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2gjn ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 10:19, 10 September 2015

OCA — Thu, 10 Sep 2015 10:19:53 GMT

←Older revision		Revision as of 10:19, 10 September 2015
Line 2:		Line 2:
	<StructureSection load='2gjn' size='340' side='right' caption='[[2gjn]], [[Resolution\|resolution]] 2.30Å' scene=''>		<StructureSection load='2gjn' size='340' side='right' caption='[[2gjn]], [[Resolution\|resolution]] 2.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2gjn]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Pseudomonas_aeruginosa_pao1 Pseudomonas aeruginosa pao1~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GJN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2GJN FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2gjn]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseae Pseae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GJN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2GJN FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene>, <scene name='pdbligand=NIS:2-NITROPROPANE'>NIS</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene>, <scene name='pdbligand=NIS:2-NITROPROPANE'>NIS</scene></td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Nitronate_monooxygenase Nitronate monooxygenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.13.12.16 1.13.12.16] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Nitronate_monooxygenase Nitronate monooxygenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.13.12.16 1.13.12.16] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2gjn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2gjn OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2gjn RCSB], [http://www.ebi.ac.uk/pdbsum/2gjn PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2gjn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2gjn OCA], [http://pdbe.org/2gjn PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2gjn RCSB], [http://www.ebi.ac.uk/pdbsum/2gjn PDBsum]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/2NPD_PSEAE 2NPD_PSEAE]] Catalyzes the oxidative denitrification of nitronates to their corresponding aldehydes and nitrites.<ref>PMID:16682407</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 2gjn" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
Line 30:		Line 33:
	</StructureSection>		</StructureSection>
	[[Category: Nitronate monooxygenase]]		[[Category: Nitronate monooxygenase]]
-	[[Category: ~~Pseudomonas aeruginosa pao1~~]]	+	[[Category: Pseae]]
	[[Category: Suh, S W]]		[[Category: Suh, S W]]
	[[Category: 2-nitropropane]]		[[Category: 2-nitropropane]]

OCA at 08:23, 16 January 2015

OCA — Fri, 16 Jan 2015 08:23:51 GMT

←Older revision		Revision as of 08:23, 16 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2gjn]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa_pao1 Pseudomonas aeruginosa pao1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GJN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2GJN FirstGlance]. <br>		<table><tr><td colspan='2'>[[2gjn]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa_pao1 Pseudomonas aeruginosa pao1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GJN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2GJN FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene>, <scene name='pdbligand=NIS:2-NITROPROPANE'>NIS</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene>, <scene name='pdbligand=NIS:2-NITROPROPANE'>NIS</scene></td></tr>
-	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Nitronate_monooxygenase Nitronate monooxygenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.13.12.16 1.13.12.16] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Nitronate_monooxygenase Nitronate monooxygenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.13.12.16 1.13.12.16] </span></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2gjn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2gjn OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2gjn RCSB], [http://www.ebi.ac.uk/pdbsum/2gjn PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2gjn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2gjn OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2gjn RCSB], [http://www.ebi.ac.uk/pdbsum/2gjn PDBsum]</span></td></tr>
-	<table>	+	</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 31:		Line 31:
	[[Category: Nitronate monooxygenase]]		[[Category: Nitronate monooxygenase]]
	[[Category: Pseudomonas aeruginosa pao1]]		[[Category: Pseudomonas aeruginosa pao1]]
-	[[Category: Suh, S W.]]	+	[[Category: Suh, S W]]
	[[Category: 2-nitropropane]]		[[Category: 2-nitropropane]]
	[[Category: 2-nitropropane dioxygenase]]		[[Category: 2-nitropropane dioxygenase]]

OCA at 08:33, 3 October 2014

OCA — Fri, 03 Oct 2014 08:33:37 GMT

←Older revision		Revision as of 08:33, 3 October 2014
Line 1:		Line 1:
-	[[~~Image~~:2gjn.~~png\|left~~\|200px]]	+	==crystal structure of 2-nitropropane dioxygenase complexed with FMN and substrate==
		+	<StructureSection load='2gjn' size='340' side='right' caption='[[2gjn]], [[Resolution\|resolution]] 2.30Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[2gjn]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa_pao1 Pseudomonas aeruginosa pao1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GJN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2GJN FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene>, <scene name='pdbligand=NIS:2-NITROPROPANE'>NIS</scene><br>
		+	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Nitronate_monooxygenase Nitronate monooxygenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.13.12.16 1.13.12.16] </span></td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2gjn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2gjn OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2gjn RCSB], [http://www.ebi.ac.uk/pdbsum/2gjn PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/gj/2gjn_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Nitroalkane compounds are widely used in chemical industry and are also produced by microorganisms and plants. Some nitroalkanes have been demonstrated to be carcinogenic, and enzymatic oxidation of nitroalkanes is of considerable interest. 2-Nitropropane dioxygenases from Neurospora crassa and Williopsis mrakii (Hansenula mrakii), members of one family of the nitroalkane-oxidizing enzymes, contain FMN and FAD, respectively. The enzymatic oxidation of nitroalkanes by 2-nitropropane dioxygenase operates by an oxidase-style catalytic mechanism, which was recently shown to involve the formation of an anionic flavin semiquinone. This represents a unique case in which an anionic flavin semiquinone has been experimentally observed in the catalytic pathway for oxidation catalyzed by a flavin-dependent enzyme. Here we report the first crystal structure of 2-nitropropane dioxygenase from Pseudomonas aeruginosa in two forms: a binary complex with FMN and a ternary complex with both FMN and 2-nitropropane. The structure identifies His(152) as the proposed catalytic base, thus providing a structural framework for a better understanding of the catalytic mechanism.

-	~~{{STRUCTURE_2gjn\| PDB=2gjn \| SCENE= }}~~	+	Crystal structure of 2-nitropropane dioxygenase complexed with FMN and substrate. Identification of the catalytic base.,Ha JY, Min JY, Lee SK, Kim HS, Kim do J, Kim KH, Lee HH, Kim HK, Yoon HJ, Suh SW J Biol Chem. 2006 Jul 7;281(27):18660-7. Epub 2006 May 8. PMID:16682407<ref>PMID:16682407</ref>

-	~~===crystal structure~~ of ~~2-nitropropane dioxygenase complexed with FMN and substrate===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_16682407}}~~	+	== References ==
-		+	<references/>
-	~~==About this Structure==~~	+	__TOC__
-	~~[[2gjn]] is a 1 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Pseudomonas_aeruginosa_pao1 Pseudomonas aeruginosa pao1]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=2GJN OCA].~~	+	</StructureSection>
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:016682407<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Nitronate monooxygenase]]		[[Category: Nitronate monooxygenase]]
	[[Category: Pseudomonas aeruginosa pao1]]		[[Category: Pseudomonas aeruginosa pao1]]

OCA: Protected "2gjn" [edit=sysop:move=sysop]

OCA — Mon, 07 Jan 2013 10:31:41 GMT

Protected "2gjn" [edit=sysop:move=sysop]

←Older revision

Revision as of 10:31, 7 January 2013

OCA at 10:31, 7 January 2013

OCA — Mon, 07 Jan 2013 10:31:37 GMT

←Older revision		Revision as of 10:31, 7 January 2013
Line 1:		Line 1:
-	{{Seed}}
	[[Image:2gjn.png\|left\|200px]]		[[Image:2gjn.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_2gjn", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_2gjn\| PDB=2gjn \| SCENE= }}		{{STRUCTURE_2gjn\| PDB=2gjn \| SCENE= }}

	===crystal structure of 2-nitropropane dioxygenase complexed with FMN and substrate===		===crystal structure of 2-nitropropane dioxygenase complexed with FMN and substrate===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_16682407}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 16682407 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_16682407}}		{{ABSTRACT_PUBMED_16682407}}

	==About this Structure==		==About this Structure==
-	~~2GJN~~ is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/~~Pseudomonas_aeruginosa~~ Pseudomonas aeruginosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GJN OCA].	+	[[2gjn]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa_pao1 Pseudomonas aeruginosa pao1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GJN OCA].

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~16682407~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:016682407</ref><references group="xtra"/>
-	[[Category: ~~2-nitropropane dioxygenase~~]]	+	[[Category: Nitronate monooxygenase]]
-	[[Category: Pseudomonas aeruginosa]]	+	[[Category: Pseudomonas aeruginosa pao1]]
	[[Category: Suh, S W.]]		[[Category: Suh, S W.]]
	[[Category: 2-nitropropane]]		[[Category: 2-nitropropane]]
		+	[[Category: 2-nitropropane dioxygenase]]
	[[Category: Fmn]]		[[Category: Fmn]]
		+	[[Category: Oxidoreductase]]
	[[Category: Substrate]]		[[Category: Substrate]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Feb 16 21:03:53 2009''