2gks - Revision history

OCA at 09:42, 30 August 2023

OCA — Wed, 30 Aug 2023 09:42:35 GMT

←Older revision		Revision as of 09:42, 30 August 2023
Line 3:		Line 3:
	<StructureSection load='2gks' size='340' side='right'caption='[[2gks]], [[Resolution\|resolution]] 2.31Å' scene=''>		<StructureSection load='2gks' size='340' side='right'caption='[[2gks]], [[Resolution\|resolution]] 2.31Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2gks]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~"aquifex_aeolicus"_huber_and_stetter_2001 "aquifex~~ aeolicus~~" huber and stetter 2001~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GKS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2GKS FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2gks]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Aquifex_aeolicus Aquifex aeolicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GKS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2GKS FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=ADP:ADENOSINE~~-~~5-DIPHOSPHATE'>ADP</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.31Å</td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat">~~sat/cysC ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode~~=~~Info&srchmode~~=5~~&id=63363 "Aquifex aeolicus" Huber and Stetter 2001])~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2gks FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2gks OCA], [https://pdbe.org/2gks PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2gks RCSB], [https://www.ebi.ac.uk/pdbsum/2gks PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2gks ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2gks FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2gks OCA], [https://pdbe.org/2gks PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2gks RCSB], [https://www.ebi.ac.uk/pdbsum/2gks PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2gks ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/SATC_AQUAE SATC_AQUAE]
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 31:		Line 33:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Aquifex aeolicus ~~huber and stetter 2001~~]]	+	[[Category: Aquifex aeolicus]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Fisher~~, A J~~]]	+	[[Category: Fisher AJ]]
-	[[Category: Lansdon~~, E B~~]]	+	[[Category: Lansdon EB]]
-	[[Category: MacRea~~, I J~~]]	+	[[Category: MacRea IJ]]
-	[[Category: Segel~~, I H~~]]	+	[[Category: Segel IH]]
-	[[Category: Yu, Z]]	+	[[Category: Yu Z]]
-	~~[[Category: Kinase]]~~	+
-	~~[[Category: Sulfurylase]]~~	+
-	~~[[Category: Transferase~~]]	+

OCA at 19:07, 10 March 2021

OCA — Wed, 10 Mar 2021 19:07:49 GMT

←Older revision		Revision as of 19:07, 10 March 2021
Line 1:		Line 1:

	==Crystal Structure of the Bi-functional ATP Sulfurylase-APS Kinase from Aquifex aeolicus, a Chemolithotrophic Thermophile==		==Crystal Structure of the Bi-functional ATP Sulfurylase-APS Kinase from Aquifex aeolicus, a Chemolithotrophic Thermophile==
-	<StructureSection load='2gks' size='340' side='right' caption='[[2gks]], [[Resolution\|resolution]] 2.31Å' scene=''>	+	<StructureSection load='2gks' size='340' side='right'caption='[[2gks]], [[Resolution\|resolution]] 2.31Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2gks]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/"aquifex_aeolicus"_huber_and_stetter_2001 "aquifex aeolicus" huber and stetter 2001]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GKS OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2GKS FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2gks]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/"aquifex_aeolicus"_huber_and_stetter_2001 "aquifex aeolicus" huber and stetter 2001]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GKS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2GKS FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">sat/cysC ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=63363 "Aquifex aeolicus" Huber and Stetter 2001])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">sat/cysC ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=63363 "Aquifex aeolicus" Huber and Stetter 2001])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2gks FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2gks OCA], [~~http~~://pdbe.org/2gks PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2gks RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2gks PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2gks ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2gks FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2gks OCA], [https://pdbe.org/2gks PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2gks RCSB], [https://www.ebi.ac.uk/pdbsum/2gks PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2gks ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 32:		Line 32:
	</StructureSection>		</StructureSection>
	[[Category: Aquifex aeolicus huber and stetter 2001]]		[[Category: Aquifex aeolicus huber and stetter 2001]]
		+	[[Category: Large Structures]]
	[[Category: Fisher, A J]]		[[Category: Fisher, A J]]
	[[Category: Lansdon, E B]]		[[Category: Lansdon, E B]]

OCA at 06:58, 20 June 2018

OCA — Wed, 20 Jun 2018 06:58:13 GMT

←Older revision		Revision as of 06:58, 20 June 2018
Line 1:		Line 1:
		+
	==Crystal Structure of the Bi-functional ATP Sulfurylase-APS Kinase from Aquifex aeolicus, a Chemolithotrophic Thermophile==		==Crystal Structure of the Bi-functional ATP Sulfurylase-APS Kinase from Aquifex aeolicus, a Chemolithotrophic Thermophile==
	<StructureSection load='2gks' size='340' side='right' caption='[[2gks]], [[Resolution\|resolution]] 2.31Å' scene=''>		<StructureSection load='2gks' size='340' side='right' caption='[[2gks]], [[Resolution\|resolution]] 2.31Å' scene=''>
Line 5:		Line 6:
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene></td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">sat/cysC ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=63363 "Aquifex aeolicus" Huber and Stetter 2001])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">sat/cysC ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=63363 "Aquifex aeolicus" Huber and Stetter 2001])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2gks FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2gks OCA], [http://pdbe.org/2gks PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2gks RCSB], [http://www.ebi.ac.uk/pdbsum/2gks PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2gks FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2gks OCA], [http://pdbe.org/2gks PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2gks RCSB], [http://www.ebi.ac.uk/pdbsum/2gks PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2gks ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 11:		Line 12:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/gk/2gks_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/gk/2gks_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2gks ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 23:00, 11 September 2015

OCA — Fri, 11 Sep 2015 23:00:59 GMT

←Older revision		Revision as of 23:00, 11 September 2015
Line 2:		Line 2:
	<StructureSection load='2gks' size='340' side='right' caption='[[2gks]], [[Resolution\|resolution]] 2.31Å' scene=''>		<StructureSection load='2gks' size='340' side='right' caption='[[2gks]], [[Resolution\|resolution]] 2.31Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2gks]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Aquifex_aeolicus Aquifex~~ aeolicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GKS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2GKS FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2gks]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"aquifex_aeolicus"_huber_and_stetter_2001 "aquifex aeolicus" huber and stetter 2001]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GKS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2GKS FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">sat/cysC ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=63363 Aquifex aeolicus])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">sat/cysC ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=63363 "Aquifex aeolicus" Huber and Stetter 2001])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2gks FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2gks OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2gks RCSB], [http://www.ebi.ac.uk/pdbsum/2gks PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2gks FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2gks OCA], [http://pdbe.org/2gks PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2gks RCSB], [http://www.ebi.ac.uk/pdbsum/2gks PDBsum]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 25:		Line 25:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 2gks" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Aquifex aeolicus]]	+	[[Category: Aquifex aeolicus huber and stetter 2001]]
	[[Category: Fisher, A J]]		[[Category: Fisher, A J]]
	[[Category: Lansdon, E B]]		[[Category: Lansdon, E B]]

OCA at 09:02, 16 January 2015

OCA — Fri, 16 Jan 2015 09:02:20 GMT

←Older revision		Revision as of 09:02, 16 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2gks]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Aquifex_aeolicus Aquifex aeolicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GKS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2GKS FirstGlance]. <br>		<table><tr><td colspan='2'>[[2gks]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Aquifex_aeolicus Aquifex aeolicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GKS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2GKS FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">sat/cysC ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=63363 Aquifex aeolicus])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">sat/cysC ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=63363 Aquifex aeolicus])</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2gks FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2gks OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2gks RCSB], [http://www.ebi.ac.uk/pdbsum/2gks PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2gks FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2gks OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2gks RCSB], [http://www.ebi.ac.uk/pdbsum/2gks PDBsum]</span></td></tr>
-	<table>	+	</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 30:		Line 30:
	</StructureSection>		</StructureSection>
	[[Category: Aquifex aeolicus]]		[[Category: Aquifex aeolicus]]
-	[[Category: Fisher, A J.]]	+	[[Category: Fisher, A J]]
-	[[Category: Lansdon, E B.]]	+	[[Category: Lansdon, E B]]
-	[[Category: MacRea, I J.]]	+	[[Category: MacRea, I J]]
-	[[Category: Segel, I H.]]	+	[[Category: Segel, I H]]
-	[[Category: Yu, Z.]]	+	[[Category: Yu, Z]]
	[[Category: Kinase]]		[[Category: Kinase]]
	[[Category: Sulfurylase]]		[[Category: Sulfurylase]]
	[[Category: Transferase]]		[[Category: Transferase]]

OCA at 10:32, 30 September 2014

OCA — Tue, 30 Sep 2014 10:32:51 GMT

←Older revision		Revision as of 10:32, 30 September 2014
Line 1:		Line 1:
-	[[~~Image:~~2gks.~~png~~\|~~left~~\|200px]]	+	==Crystal Structure of the Bi-functional ATP Sulfurylase-APS Kinase from Aquifex aeolicus, a Chemolithotrophic Thermophile==
		+	<StructureSection load='2gks' size='340' side='right' caption='[[2gks]], [[Resolution\|resolution]] 2.31Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[2gks]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Aquifex_aeolicus Aquifex aeolicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GKS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2GKS FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">sat/cysC ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=63363 Aquifex aeolicus])</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2gks FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2gks OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2gks RCSB], [http://www.ebi.ac.uk/pdbsum/2gks PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/gk/2gks_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The thermophilic chemolithotroph, Aquifex aeolicus, expresses a gene product that exhibits both ATP sulfurylase and adenosine-5'-phosphosulfate (APS) kinase activities. These enzymes are usually segregated on two separate proteins in most bacteria, fungi, and plants. The domain arrangement in the Aquifex enzyme is reminiscent of the fungal ATP sulfurylase, which contains a C-terminal domain that is homologous to APS kinase yet displays no kinase activity. Rather, in the fungal enzyme, the motif serves as a sulfurylase regulatory domain that binds the allosteric effector 3'-phosphoadenosine-5'-phosphosulfate (PAPS), the product of true APS kinase. Therefore, the Aquifex enzyme may represent an ancestral homolog of a primitive bifunctional enzyme, from which the fungal ATP sulfurylase may have evolved. In heterotrophic sulfur-assimilating organisms such as fungi, ATP sulfurylase catalyzes the first committed step in sulfate assimilation to produce APS, which is subsequently metabolized to generate all sulfur-containing biomolecules. In contrast, ATP sulfurylase in sulfur chemolithotrophs catalyzes the reverse reaction to produce ATP and sulfate from APS and pyrophosphate. Here, the 2.3 A resolution X-ray crystal structure of Aquifex ATP sulfurylase-APS kinase bifunctional enzyme is presented. The protein dimerizes through its APS kinase domain and contains ADP bound in all four active sites. Comparison of the Aquifex ATP sulfurylase active site with those from sulfate assimilators reveals similar dispositions of the bound nucleotide and nearby residues. This suggests that minor perturbations are responsible for optimizing the kinetic properties for the physiologically relevant direction. The APS kinase active-site lid adopts two distinct conformations, where one conformation is distorted by crystal contacts. Additionally, a disulfide bond is observed in one ATP-binding P-loop of the APS kinase active site. This linkage accounts for the low kinase activity of the enzyme under oxidizing conditions. The thermal stability of the Aquifex enzyme can be explained by the 43% decreased cavity volume found within the protein core.

-	~~{{STRUCTURE_2gks\| PDB=2gks \| SCENE= }}~~	+	Crystal structure of the bifunctional ATP sulfurylase-APS kinase from the chemolithotrophic thermophile Aquifex aeolicus.,Yu Z, Lansdon EB, Segel IH, Fisher AJ J Mol Biol. 2007 Jan 19;365(3):732-43. Epub 2006 Oct 14. PMID:17095009<ref>PMID:17095009</ref>

-	~~===Crystal Structure~~ of the ~~Bi-functional ATP Sulfurylase-APS Kinase from Aquifex aeolicus, a Chemolithotrophic Thermophile===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_17095009}}~~	+	== References ==
-		+	<references/>
-	~~==About this Structure==~~	+	__TOC__
-	~~[[2gks]] is a 2 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Aquifex_aeolicus Aquifex aeolicus]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=2GKS OCA].~~	+	</StructureSection>
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:017095009<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Aquifex aeolicus]]		[[Category: Aquifex aeolicus]]
	[[Category: Fisher, A J.]]		[[Category: Fisher, A J.]]

OCA: Protected "2gks" [edit=sysop:move=sysop]

OCA — Mon, 07 Jan 2013 12:23:39 GMT

Protected "2gks" [edit=sysop:move=sysop]

←Older revision

Revision as of 12:23, 7 January 2013

OCA at 12:23, 7 January 2013

OCA — Mon, 07 Jan 2013 12:23:38 GMT

←Older revision		Revision as of 12:23, 7 January 2013
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-	{{Seed}}
	[[Image:2gks.png\|left\|200px]]		[[Image:2gks.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_2gks", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
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	{{STRUCTURE_2gks\| PDB=2gks \| SCENE= }}		{{STRUCTURE_2gks\| PDB=2gks \| SCENE= }}

	===Crystal Structure of the Bi-functional ATP Sulfurylase-APS Kinase from Aquifex aeolicus, a Chemolithotrophic Thermophile===		===Crystal Structure of the Bi-functional ATP Sulfurylase-APS Kinase from Aquifex aeolicus, a Chemolithotrophic Thermophile===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_17095009}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 17095009 is the PubMed ID number.
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	{{ABSTRACT_PUBMED_17095009}}		{{ABSTRACT_PUBMED_17095009}}

	==About this Structure==		==About this Structure==
-	~~2GKS~~ is a 2 ~~chains~~ structure ~~of sequences~~ from [http://en.wikipedia.org/wiki/Aquifex_aeolicus Aquifex aeolicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GKS OCA].	+	[[2gks]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Aquifex_aeolicus Aquifex aeolicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GKS OCA].

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~17095009~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:017095009</ref><references group="xtra"/>
	[[Category: Aquifex aeolicus]]		[[Category: Aquifex aeolicus]]
	[[Category: Fisher, A J.]]		[[Category: Fisher, A J.]]
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	[[Category: Sulfurylase]]		[[Category: Sulfurylase]]
	[[Category: Transferase]]		[[Category: Transferase]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Feb 18 06:26:53 2009''

OCA at 04:26, 18 February 2009

OCA — Wed, 18 Feb 2009 04:26:55 GMT

←Older revision		Revision as of 04:26, 18 February 2009
Line 20:		Line 20:

	==About this Structure==		==About this Structure==
-	2GKS is a ~~[[Single protein]]~~ structure of ~~sequence~~ from [http://en.wikipedia.org/wiki/Aquifex_aeolicus Aquifex aeolicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GKS OCA].	+	2GKS is a 2 chains structure of sequences from [http://en.wikipedia.org/wiki/Aquifex_aeolicus Aquifex aeolicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GKS OCA].

	==Reference==		==Reference==
-	Crystal structure of the bifunctional ATP sulfurylase-APS kinase from the chemolithotrophic thermophile Aquifex aeolicus., Yu Z, Lansdon EB, Segel IH, Fisher AJ, J Mol Biol. 2007 Jan 19;365(3):732-43. Epub 2006 Oct 14. PMID:~~[http:~~//~~www.ncbi.nlm.nih.gov/pubmed/17095009 17095009]~~	+	<ref group="xtra">PMID:17095009</ref><references group="xtra"/>
	[[Category: Aquifex aeolicus]]		[[Category: Aquifex aeolicus]]
-	[[Category: Single protein]]
	[[Category: Fisher, A J.]]		[[Category: Fisher, A J.]]
	[[Category: Lansdon, E B.]]		[[Category: Lansdon, E B.]]
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	[[Category: Transferase]]		[[Category: Transferase]]

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Tue Jul 29 13~~:10:~~00 2008~~''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Feb 18 06:26:53 2009''

OCA at 10:10, 29 July 2008

OCA — Tue, 29 Jul 2008 10:10:03 GMT

←Older revision		Revision as of 10:10, 29 July 2008
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-	[[Image:2gks.~~jpg~~\|left\|200px]]	+	{{Seed}}
		+	[[Image:2gks.png\|left\|200px]]

	<!--		<!--
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	{{STRUCTURE_2gks\| PDB=2gks \| SCENE= }}		{{STRUCTURE_2gks\| PDB=2gks \| SCENE= }}

-	~~'''~~Crystal Structure of the Bi-functional ATP Sulfurylase-APS Kinase from Aquifex aeolicus, a Chemolithotrophic Thermophile~~'''~~	+	===Crystal Structure of the Bi-functional ATP Sulfurylase-APS Kinase from Aquifex aeolicus, a Chemolithotrophic Thermophile===


-	~~==Overview==~~	+	<!--
-	The ~~thermophilic chemolithotroph~~, ~~Aquifex aeolicus~~, expresses a gene product that exhibits both ATP sulfurylase and adenosine-5'-phosphosulfate (APS) kinase activities. These enzymes are usually segregated on two separate proteins in most bacteria, fungi, and plants. The domain arrangement in the ~~Aquifex enzyme is reminiscent of the fungal ATP sulfurylase, which contains a C-terminal domain that is homologous~~ to ~~APS kinase yet displays no kinase activity. Rather, in~~ the ~~fungal enzyme, the motif serves as a sulfurylase regulatory domain that binds the allosteric effector 3'-phosphoadenosine-5'-phosphosulfate~~ (PAPS), the product of true APS kinase. Therefore, the Aquifex enzyme may represent an ancestral homolog of a primitive bifunctional enzyme, from which the fungal ATP sulfurylase may have evolved. In heterotrophic sulfur-assimilating organisms such as ~~fungi, ATP sulfurylase catalyzes the first committed step in sulfate assimilation to produce APS, which is subsequently metabolized to generate all sulfur-containing biomolecules~~. ~~In contrast, ATP sulfurylase in sulfur chemolithotrophs catalyzes the reverse reaction to produce ATP and sulfate from APS and pyrophosphate~~. ~~Here~~, ~~the 2.3 A resolution X-ray crystal structure of Aquifex ATP sulfurylase-APS kinase bifunctional enzyme~~ is ~~presented. The protein dimerizes through its APS kinase domain and contains ADP bound in all four active sites. Comparison of~~ the ~~Aquifex ATP sulfurylase active site with those from sulfate assimilators reveals similar dispositions of the bound nucleotide and nearby residues~~. ~~This suggests that minor perturbations are responsible for optimizing the kinetic properties for the physiologically relevant direction. The APS kinase active~~-~~site lid adopts two distinct conformations, where one conformation is distorted by crystal contacts. Additionally, a disulfide bond is observed in one ATP~~-binding P-loop of the APS kinase active site. This linkage accounts for the low kinase activity of the enzyme under oxidizing conditions. The thermal stability of the Aquifex enzyme can be explained by the 43% decreased cavity volume found within the protein core.	+	The line below this paragraph, {{ABSTRACT_PUBMED_17095009}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 17095009 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_17095009}}

	==About this Structure==		==About this Structure==
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	[[Category: Sulfurylase]]		[[Category: Sulfurylase]]
	[[Category: Transferase]]		[[Category: Transferase]]
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