2h7t - Revision history

OCA at 07:33, 17 March 2021

OCA — Wed, 17 Mar 2021 07:33:45 GMT

←Older revision		Revision as of 07:33, 17 March 2021
Line 1:		Line 1:

	==Solution Structure of the C-terminal Domain of Insulin-like Growth Factor Binding Protein 2 (IGFBP-2)==		==Solution Structure of the C-terminal Domain of Insulin-like Growth Factor Binding Protein 2 (IGFBP-2)==
-	<StructureSection load='2h7t' size='340' side='right' caption='[[2h7t]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>	+	<StructureSection load='2h7t' size='340' side='right'caption='[[2h7t]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2h7t]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Human Human]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2H7T OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2H7T FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2h7t]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Human Human]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2H7T OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2H7T FirstGlance]. <br>
-	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">IGFBP2, BP2 ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>	+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">IGFBP2, BP2 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2h7t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2h7t OCA], [~~http~~://pdbe.org/2h7t PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2h7t RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2h7t PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2h7t ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2h7t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2h7t OCA], [https://pdbe.org/2h7t PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2h7t RCSB], [https://www.ebi.ac.uk/pdbsum/2h7t PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2h7t ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/IBP2_HUMAN IBP2_HUMAN]] Inhibits IGF-mediated growth and developmental rates. IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors.<ref>PMID:19081843</ref>	+	[[https://www.uniprot.org/uniprot/IBP2_HUMAN IBP2_HUMAN]] Inhibits IGF-mediated growth and developmental rates. IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors.<ref>PMID:19081843</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 30:		Line 30:

	==See Also==		==See Also==
-	*[[Insulin-like growth factor binding protein\|Insulin-like growth factor binding protein]]	+	*[[Insulin-like growth factor binding protein 3D structures\|Insulin-like growth factor binding protein 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 36:		Line 36:
	</StructureSection>		</StructureSection>
	[[Category: Human]]		[[Category: Human]]
		+	[[Category: Large Structures]]
	[[Category: Kuang, Z]]		[[Category: Kuang, Z]]
	[[Category: Protein binding]]		[[Category: Protein binding]]
	[[Category: Thyroglobulin type 1 fold]]		[[Category: Thyroglobulin type 1 fold]]

OCA at 06:42, 27 June 2018

OCA — Wed, 27 Jun 2018 06:42:02 GMT

←Older revision		Revision as of 06:42, 27 June 2018
Line 1:		Line 1:
		+
	==Solution Structure of the C-terminal Domain of Insulin-like Growth Factor Binding Protein 2 (IGFBP-2)==		==Solution Structure of the C-terminal Domain of Insulin-like Growth Factor Binding Protein 2 (IGFBP-2)==
	<StructureSection load='2h7t' size='340' side='right' caption='[[2h7t]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>		<StructureSection load='2h7t' size='340' side='right' caption='[[2h7t]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
Line 4:		Line 5:
	<table><tr><td colspan='2'>[[2h7t]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2H7T OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2H7T FirstGlance]. <br>		<table><tr><td colspan='2'>[[2h7t]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2H7T OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2H7T FirstGlance]. <br>
	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">IGFBP2, BP2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>		</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">IGFBP2, BP2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2h7t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2h7t OCA], [http://pdbe.org/2h7t PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2h7t RCSB], [http://www.ebi.ac.uk/pdbsum/2h7t PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2h7t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2h7t OCA], [http://pdbe.org/2h7t PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2h7t RCSB], [http://www.ebi.ac.uk/pdbsum/2h7t PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2h7t ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 12:		Line 13:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/h7/2h7t_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/h7/2h7t_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2h7t ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 27:		Line 28:
	</div>		</div>
	<div class="pdbe-citations 2h7t" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 2h7t" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Insulin-like growth factor binding protein\|Insulin-like growth factor binding protein]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 11:33, 11 September 2015

OCA — Fri, 11 Sep 2015 11:33:42 GMT

←Older revision		Revision as of 11:33, 11 September 2015
Line 2:		Line 2:
	<StructureSection load='2h7t' size='340' side='right' caption='[[2h7t]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>		<StructureSection load='2h7t' size='340' side='right' caption='[[2h7t]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2h7t]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Homo_sapiens Homo sapiens~~]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2H7T OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2H7T FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2h7t]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2H7T OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2H7T FirstGlance]. <br>
-	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">IGFBP2, BP2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 ~~Homo sapiens~~])</td></tr>	+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">IGFBP2, BP2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2h7t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2h7t OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2h7t RCSB], [http://www.ebi.ac.uk/pdbsum/2h7t PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2h7t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2h7t OCA], [http://pdbe.org/2h7t PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2h7t RCSB], [http://www.ebi.ac.uk/pdbsum/2h7t PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 26:		Line 26:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 2h7t" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Homo sapiens~~]]	+	[[Category: Human]]
	[[Category: Kuang, Z]]		[[Category: Kuang, Z]]
	[[Category: Protein binding]]		[[Category: Protein binding]]
	[[Category: Thyroglobulin type 1 fold]]		[[Category: Thyroglobulin type 1 fold]]

OCA at 15:54, 25 December 2014

OCA — Thu, 25 Dec 2014 15:54:25 GMT

←Older revision		Revision as of 15:54, 25 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2h7t]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2H7T OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2H7T FirstGlance]. <br>		<table><tr><td colspan='2'>[[2h7t]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2H7T OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2H7T FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">IGFBP2, BP2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])</td></tr>	+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">IGFBP2, BP2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2h7t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2h7t OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2h7t RCSB], [http://www.ebi.ac.uk/pdbsum/2h7t PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2h7t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2h7t OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2h7t RCSB], [http://www.ebi.ac.uk/pdbsum/2h7t PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/IBP2_HUMAN IBP2_HUMAN]] Inhibits IGF-mediated growth and developmental rates. IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors.<ref>PMID:19081843</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 29:		Line 31:
	</StructureSection>		</StructureSection>
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
-	[[Category: Kuang, Z.]]	+	[[Category: Kuang, Z]]
	[[Category: Protein binding]]		[[Category: Protein binding]]
	[[Category: Thyroglobulin type 1 fold]]		[[Category: Thyroglobulin type 1 fold]]

OCA at 08:54, 30 September 2014

OCA — Tue, 30 Sep 2014 08:54:19 GMT

←Older revision		Revision as of 08:54, 30 September 2014
Line 1:		Line 1:
-	[[~~Image~~:2h7t.~~png\|left~~\|200px]]	+	==Solution Structure of the C-terminal Domain of Insulin-like Growth Factor Binding Protein 2 (IGFBP-2)==
		+	<StructureSection load='2h7t' size='340' side='right' caption='[[2h7t]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[2h7t]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2H7T OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2H7T FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">IGFBP2, BP2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2h7t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2h7t OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2h7t RCSB], [http://www.ebi.ac.uk/pdbsum/2h7t PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/h7/2h7t_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Insulin-like growth factor-binding protein-2 (IGFBP-2) is the largest member of a family of six proteins (IGFBP-1 to 6) that bind insulin-like growth factors I and II (IGF-I/II) with high affinity. In addition to regulating IGF actions, IGFBPs have IGF-independent functions. The C-terminal domains of IGFBPs contribute to high-affinity IGF binding, and confer binding specificity and have overlapping but variable interactions with many other molecules. Using nuclear magnetic resonance (NMR) spectroscopy, we have determined the solution structure of the C-terminal domain of IGFBP-2 (C-BP-2) and analysed its backbone dynamics based on 15N relaxation parameters. C-BP-2 has a thyroglobulin type 1 fold consisting of an alpha-helix, a three-stranded anti-parallel beta-sheet and three flexible loops. Compared to C-BP-6 and C-BP-1, structural differences that may affect IGF binding and underlie other functional differences were found. C-BP-2 has a longer disordered loop I, and an extended C-terminal tail, which is unstructured and very mobile. The length of the helix is identical with that of C-BP-6 but shorter than that of C-BP-1. Reduced spectral density mapping analysis showed that C-BP-2 possesses significant rapid motion in the loops and termini, and may undergo slower conformational or chemical exchange in the structured core and loop II. An RGD motif is located in a solvent-exposed turn. A pH-dependent heparin-binding site on C-BP-2 has been identified. Protonation of two histidine residues, His271 and His228, seems to be important for this binding, which occurs at slightly acidic pH (6.0) and is more significant at pH 5.5, but is largely suppressed at pH 7.4. Possible preferential binding of IGFBP-2 and its C- domain fragments to glycosaminoglycans in the acidic extracellular matrix (ECM) of tumours may be related to their roles in cancer.

-	~~{{STRUCTURE_2h7t\| PDB=2h7t \| SCENE= }}~~	+	Structure, dynamics and heparin binding of the C-terminal domain of insulin-like growth factor-binding protein-2 (IGFBP-2).,Kuang Z, Yao S, Keizer DW, Wang CC, Bach LA, Forbes BE, Wallace JC, Norton RS J Mol Biol. 2006 Dec 8;364(4):690-704. Epub 2006 Sep 7. PMID:17020769<ref>PMID:17020769</ref>

-	~~===Solution Structure~~ of the ~~C-terminal Domain of Insulin-like Growth Factor Binding Protein 2 (IGFBP-2)===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_17020769}}~~	+	== References ==
-		+	<references/>
-	~~==About this Structure==~~	+	__TOC__
-	~~[[2h7t]] is a 1 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Homo_sapiens Homo sapiens]~~. ~~Full experimental information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=2H7T OCA].~~	+	</StructureSection>
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:017020769<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
	[[Category: Kuang, Z.]]		[[Category: Kuang, Z.]]
	[[Category: Protein binding]]		[[Category: Protein binding]]
	[[Category: Thyroglobulin type 1 fold]]		[[Category: Thyroglobulin type 1 fold]]

OCA: Protected "2h7t" [edit=sysop:move=sysop]

OCA — Mon, 07 Jan 2013 11:14:24 GMT

Protected "2h7t" [edit=sysop:move=sysop]

←Older revision

Revision as of 11:14, 7 January 2013

OCA at 11:14, 7 January 2013

OCA — Mon, 07 Jan 2013 11:14:21 GMT

←Older revision		Revision as of 11:14, 7 January 2013
Line 1:		Line 1:
-	{{Seed}}
	[[Image:2h7t.png\|left\|200px]]		[[Image:2h7t.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_2h7t", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_2h7t\| PDB=2h7t \| SCENE= }}		{{STRUCTURE_2h7t\| PDB=2h7t \| SCENE= }}

	===Solution Structure of the C-terminal Domain of Insulin-like Growth Factor Binding Protein 2 (IGFBP-2)===		===Solution Structure of the C-terminal Domain of Insulin-like Growth Factor Binding Protein 2 (IGFBP-2)===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_17020769}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 17020769 is the PubMed ID number.
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	{{ABSTRACT_PUBMED_17020769}}		{{ABSTRACT_PUBMED_17020769}}

	==About this Structure==		==About this Structure==
-	~~2H7T~~ is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2H7T OCA].	+	[[2h7t]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2H7T OCA].

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~17020769~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:017020769</ref><references group="xtra"/>
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
	[[Category: Kuang, Z.]]		[[Category: Kuang, Z.]]
		+	[[Category: Protein binding]]
	[[Category: Thyroglobulin type 1 fold]]		[[Category: Thyroglobulin type 1 fold]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 21:01:13 2009''

OCA at 19:01, 17 February 2009

OCA — Tue, 17 Feb 2009 19:01:15 GMT

←Older revision		Revision as of 19:01, 17 February 2009
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	==About this Structure==		==About this Structure==
-	2H7T is a ~~[[Single protein]]~~ structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2H7T OCA].	+	2H7T is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2H7T OCA].

	==Reference==		==Reference==
-	Structure, dynamics and heparin binding of the C-terminal domain of insulin-like growth factor-binding protein-2 (IGFBP-2)., Kuang Z, Yao S, Keizer DW, Wang CC, Bach LA, Forbes BE, Wallace JC, Norton RS, J Mol Biol. 2006 Dec 8;364(4):690-704. Epub 2006 Sep 7. PMID:~~[http:~~//~~www.ncbi.nlm.nih.gov/pubmed/17020769 17020769]~~	+	<ref group="xtra">PMID:17020769</ref><references group="xtra"/>
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
-	[[Category: Single protein]]
	[[Category: Kuang, Z.]]		[[Category: Kuang, Z.]]
	[[Category: Thyroglobulin type 1 fold]]		[[Category: Thyroglobulin type 1 fold]]

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue ~~Jul 29 05~~:02:~~44 2008~~''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 21:01:13 2009''

OCA at 02:02, 29 July 2008

OCA — Tue, 29 Jul 2008 02:02:52 GMT

←Older revision		Revision as of 02:02, 29 July 2008
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-	[[Image:2h7t.~~jpg~~\|left\|200px]]	+	{{Seed}}
		+	[[Image:2h7t.png\|left\|200px]]

	<!--		<!--
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	{{STRUCTURE_2h7t\| PDB=2h7t \| SCENE= }}		{{STRUCTURE_2h7t\| PDB=2h7t \| SCENE= }}

-	~~'''~~Solution Structure of the C-terminal Domain of Insulin-like Growth Factor Binding Protein 2 (IGFBP-2)~~'''~~	+	===Solution Structure of the C-terminal Domain of Insulin-like Growth Factor Binding Protein 2 (IGFBP-2)===


-	~~==Overview==~~	+	<!--
-	~~Insulin~~-~~like growth factor~~-binding protein-2 (IGFBP-2) is the largest member of a family of six proteins (IGFBP-1 to 6) that bind insulin-like growth factors I and II (IGF-I/II) with high affinity. In addition to regulating IGF actions, IGFBPs have IGF-independent functions. The ~~C-terminal domains of IGFBPs contribute to high-affinity IGF binding~~, ~~and confer binding specificity and have overlapping but variable interactions with many other molecules. Using nuclear magnetic resonance (NMR) spectroscopy~~, ~~we have determined~~ the ~~solution structure of~~ the ~~C-terminal domain of IGFBP-2~~ (~~C-BP-2) and analysed its backbone dynamics based~~ on ~~15N relaxation parameters~~. ~~C-BP-2 has a thyroglobulin type 1 fold consisting of an alpha-helix, a three-stranded anti-parallel beta-sheet and three flexible loops~~. Compared to C-BP-6 and C-BP-1, structural differences that may affect IGF binding and underlie other functional differences were found. C-BP-2 has a longer disordered loop I, and an extended C-terminal tail, ~~which~~ is ~~unstructured and very mobile. The length of~~ the ~~helix is identical with that of C-BP-6 but shorter than that of C-BP-1~~. ~~Reduced spectral density mapping analysis showed that C~~-BP-2 possesses significant rapid motion in the loops and termini, and may undergo slower conformational or chemical exchange in the structured core and loop II. An RGD motif is located in a solvent-exposed turn. A pH-dependent heparin-binding site on C-BP-2 has been identified. Protonation of two histidine residues, His271 and His228, seems to be important for this binding, which occurs at slightly acidic pH (6.0) and is more significant at pH 5.5, but is largely suppressed at pH 7.4. Possible preferential binding of IGFBP-2 and its C- domain fragments to glycosaminoglycans in the acidic extracellular matrix (ECM) of tumours may be related to their roles in cancer.	+	The line below this paragraph, {{ABSTRACT_PUBMED_17020769}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 17020769 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_17020769}}

	==About this Structure==		==About this Structure==
-	2H7T is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full ~~crystallographic~~ information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2H7T OCA].	+	2H7T is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2H7T OCA].

	==Reference==		==Reference==
Line 24:		Line 28:
	[[Category: Kuang, Z.]]		[[Category: Kuang, Z.]]
	[[Category: Thyroglobulin type 1 fold]]		[[Category: Thyroglobulin type 1 fold]]
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Sun May 4~~ 05:58:25 2008''	+
		+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 05:02:44 2008''

OCA at 02:58, 4 May 2008

OCA — Sun, 04 May 2008 02:58:26 GMT

←Older revision		Revision as of 02:58, 4 May 2008
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	[[Image:2h7t.jpg\|left\|200px]]		[[Image:2h7t.jpg\|left\|200px]]

-	{{Structure	+	<!--
-	\|PDB~~= 2h7t \|SIZE=350\|CAPTION= <scene name='initialview01'>2h7t</scene>~~	+	The line below this paragraph, containing "STRUCTURE_2h7t", creates the "Structure Box" on the page.
-	~~\|SITE=~~	+	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	~~\|LIGAND=~~	+	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	~~\|ACTIVITY=~~	+	or leave the SCENE parameter empty for the default display.
-	~~\|GENE= IGFBP2, BP2~~ (~~[http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens]~~)	+	-->
-	~~\|DOMAIN=~~	+	{{STRUCTURE_2h7t\| PDB=2h7t \| SCENE= }}
-	~~\|RELATEDENTRY=~~	+
-	\|~~RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol~~=2h7t ~~FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id~~=~~2h7t OCA], [http://www.ebi.ac.uk/pdbsum/2h7t PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2h7t RCSB]</span>~~	+
-	}}	+

	'''Solution Structure of the C-terminal Domain of Insulin-like Growth Factor Binding Protein 2 (IGFBP-2)'''		'''Solution Structure of the C-terminal Domain of Insulin-like Growth Factor Binding Protein 2 (IGFBP-2)'''
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	[[Category: Single protein]]		[[Category: Single protein]]
	[[Category: Kuang, Z.]]		[[Category: Kuang, Z.]]
-	[[Category: ~~thyroglobulin~~ type 1 fold]]	+	[[Category: Thyroglobulin type 1 fold]]
-		+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 05:58:25 2008''
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Mon Mar 31 03~~:26:43 2008''	+