2h8q - Revision history

OCA at 09:53, 30 August 2023

OCA — Wed, 30 Aug 2023 09:53:58 GMT

←Older revision		Revision as of 09:53, 30 August 2023
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	<StructureSection load='2h8q' size='340' side='right'caption='[[2h8q]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='2h8q' size='340' side='right'caption='[[2h8q]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2h8q]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Dissp Dissp~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2H8Q OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2H8Q FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2h8q]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Discosoma_sp. Discosoma sp.]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2H8Q OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2H8Q FirstGlance]. <br>
-	</td></tr><tr id='~~NonStdRes~~'><td class="sblockLbl"><b>[[~~Non-Standard_Residue~~\|~~NonStd Res~~:]]</b></td><td class="sblockDat">~~<scene name='pdbligand=CRQ:[2~~-~~(3-CARBAMOYL-1-IMINO-PROPYL)-4-(4-HYDROXY-BENZYLIDENE)-5-OXO-4~~,~~5-DIHYDRO-IMIDAZOL-1-YL~~]~~-ACETIC+ACID'>CRQ</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2Å</td></tr>
-	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"><~~div style~~='~~overflow~~: ~~auto; max~~-~~height: 3em;'>[[1g7k\|1g7k]]~~, ~~[[2h5o\|2h5o~~]~~], [[2h5p\|2h5p]], [[2h5q\|2h5q]], [[2h5r\|2h5r]]</div></td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CRQ:[2-(3-CARBAMOYL-1-IMINO-PROPYL)-4-(4-HYDROXY-BENZYLIDENE)-5-OXO-4,5-DIHYDRO-IMIDAZOL-1-YL]-ACETIC+ACID'>CRQ</scene></td></tr>
-	~~<tr id='gene~~'>~~<td class="sblockLbl"><b>[[Gene\|Gene:]]~~</b>~~</td><td class="sblockDat">dRFP583 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=86600 DISSP])~~</td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2h8q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2h8q OCA], [https://pdbe.org/2h8q PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2h8q RCSB], [https://www.ebi.ac.uk/pdbsum/2h8q PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2h8q ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2h8q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2h8q OCA], [https://pdbe.org/2h8q PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2h8q RCSB], [https://www.ebi.ac.uk/pdbsum/2h8q PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2h8q ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/RFP_DISSP RFP_DISSP]] Thought to play a role in photoprotection of the coral's resident symbiont microalgae's photosystems from photoinhibition caused by high light levels found near the surface of coral reefs. In deeper water, the fluorescence may be to convert blue light into longer wavelengths more suitable for use in photosynthesis by the microalgal symbionts.<ref>PMID:10504696</ref>	+	[https://www.uniprot.org/uniprot/RFP_DISSP RFP_DISSP] Thought to play a role in photoprotection of the coral's resident symbiont microalgae's photosystems from photoinhibition caused by high light levels found near the surface of coral reefs. In deeper water, the fluorescence may be to convert blue light into longer wavelengths more suitable for use in photosynthesis by the microalgal symbionts.<ref>PMID:10504696</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Dissp~~]]	+	[[Category: Discosoma sp]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Remington~~, S J~~]]	+	[[Category: Remington SJ]]
-	[[Category: Yarbrough~~, C A]]~~	+	[[Category: Yarbrough CA]]
-	~~[[Category: Beta barrel]]~~	+
-	~~[[Category: Bioluminescence]]~~	+
-	~~[[Category: Dsred]]~~	+
-	~~[[Category: Fluorescent]]~~	+
-	~~[[Category: Gfp]]~~	+
-	~~[[Category: Luminescent protein]]~~	+
-	~~[[Category: Red shift~~]]	+

OCA at 07:34, 17 March 2021

OCA — Wed, 17 Mar 2021 07:34:14 GMT

←Older revision		Revision as of 07:34, 17 March 2021
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	==Crystal Structure of a Redshifted Mutant (K83M) of the Red Fluorescent Protein dRFP583/dsRed==		==Crystal Structure of a Redshifted Mutant (K83M) of the Red Fluorescent Protein dRFP583/dsRed==
-	<StructureSection load='2h8q' size='340' side='right' caption='[[2h8q]], [[Resolution\|resolution]] 2.00Å' scene=''>	+	<StructureSection load='2h8q' size='340' side='right'caption='[[2h8q]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2h8q]] is a 4 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Dissp Dissp]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2H8Q OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2H8Q FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2h8q]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Dissp Dissp]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2H8Q OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2H8Q FirstGlance]. <br>
	</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CRQ:[2-(3-CARBAMOYL-1-IMINO-PROPYL)-4-(4-HYDROXY-BENZYLIDENE)-5-OXO-4,5-DIHYDRO-IMIDAZOL-1-YL]-ACETIC+ACID'>CRQ</scene></td></tr>		</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CRQ:[2-(3-CARBAMOYL-1-IMINO-PROPYL)-4-(4-HYDROXY-BENZYLIDENE)-5-OXO-4,5-DIHYDRO-IMIDAZOL-1-YL]-ACETIC+ACID'>CRQ</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1g7k\|1g7k]], [[2h5o\|2h5o]], [[2h5p\|2h5p]], [[2h5q\|2h5q]], [[2h5r\|2h5r]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1g7k\|1g7k]], [[2h5o\|2h5o]], [[2h5p\|2h5p]], [[2h5q\|2h5q]], [[2h5r\|2h5r]]</div></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">dRFP583 ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=86600 DISSP])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">dRFP583 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=86600 DISSP])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2h8q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2h8q OCA], [~~http~~://pdbe.org/2h8q PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2h8q RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2h8q PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2h8q ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2h8q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2h8q OCA], [https://pdbe.org/2h8q PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2h8q RCSB], [https://www.ebi.ac.uk/pdbsum/2h8q PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2h8q ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/RFP_DISSP RFP_DISSP]] Thought to play a role in photoprotection of the coral's resident symbiont microalgae's photosystems from photoinhibition caused by high light levels found near the surface of coral reefs. In deeper water, the fluorescence may be to convert blue light into longer wavelengths more suitable for use in photosynthesis by the microalgal symbionts.<ref>PMID:10504696</ref>	+	[[https://www.uniprot.org/uniprot/RFP_DISSP RFP_DISSP]] Thought to play a role in photoprotection of the coral's resident symbiont microalgae's photosystems from photoinhibition caused by high light levels found near the surface of coral reefs. In deeper water, the fluorescence may be to convert blue light into longer wavelengths more suitable for use in photosynthesis by the microalgal symbionts.<ref>PMID:10504696</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 32:		Line 32:

	==See Also==		==See Also==
-	*[[Green Fluorescent Protein\|Green Fluorescent Protein]]	+	*[[Green Fluorescent Protein 3D structures\|Green Fluorescent Protein 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 38:		Line 38:
	</StructureSection>		</StructureSection>
	[[Category: Dissp]]		[[Category: Dissp]]
		+	[[Category: Large Structures]]
	[[Category: Remington, S J]]		[[Category: Remington, S J]]
	[[Category: Yarbrough, C A]]		[[Category: Yarbrough, C A]]

OCA at 06:12, 27 June 2018

OCA — Wed, 27 Jun 2018 06:12:44 GMT

←Older revision		Revision as of 06:12, 27 June 2018
Line 1:		Line 1:
		+
	==Crystal Structure of a Redshifted Mutant (K83M) of the Red Fluorescent Protein dRFP583/dsRed==		==Crystal Structure of a Redshifted Mutant (K83M) of the Red Fluorescent Protein dRFP583/dsRed==
	<StructureSection load='2h8q' size='340' side='right' caption='[[2h8q]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='2h8q' size='340' side='right' caption='[[2h8q]], [[Resolution\|resolution]] 2.00Å' scene=''>
Line 6:		Line 7:
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1g7k\|1g7k]], [[2h5o\|2h5o]], [[2h5p\|2h5p]], [[2h5q\|2h5q]], [[2h5r\|2h5r]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1g7k\|1g7k]], [[2h5o\|2h5o]], [[2h5p\|2h5p]], [[2h5q\|2h5q]], [[2h5r\|2h5r]]</td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">dRFP583 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=86600 DISSP])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">dRFP583 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=86600 DISSP])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2h8q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2h8q OCA], [http://pdbe.org/2h8q PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2h8q RCSB], [http://www.ebi.ac.uk/pdbsum/2h8q PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2h8q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2h8q OCA], [http://pdbe.org/2h8q PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2h8q RCSB], [http://www.ebi.ac.uk/pdbsum/2h8q PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2h8q ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 14:		Line 15:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/h8/2h8q_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/h8/2h8q_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>

OCA at 22:09, 7 February 2016

OCA — Sun, 07 Feb 2016 22:09:43 GMT

←Older revision		Revision as of 22:09, 7 February 2016
Line 18:		Line 18:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2h8q ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 05:22, 10 September 2015

OCA — Thu, 10 Sep 2015 05:22:47 GMT

←Older revision		Revision as of 05:22, 10 September 2015
Line 2:		Line 2:
	<StructureSection load='2h8q' size='340' side='right' caption='[[2h8q]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='2h8q' size='340' side='right' caption='[[2h8q]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2h8q]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Discosoma_sp. Discosoma sp.~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2H8Q OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2H8Q FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2h8q]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Dissp Dissp]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2H8Q OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2H8Q FirstGlance]. <br>
	</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CRQ:[2-(3-CARBAMOYL-1-IMINO-PROPYL)-4-(4-HYDROXY-BENZYLIDENE)-5-OXO-4,5-DIHYDRO-IMIDAZOL-1-YL]-ACETIC+ACID'>CRQ</scene></td></tr>		</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CRQ:[2-(3-CARBAMOYL-1-IMINO-PROPYL)-4-(4-HYDROXY-BENZYLIDENE)-5-OXO-4,5-DIHYDRO-IMIDAZOL-1-YL]-ACETIC+ACID'>CRQ</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1g7k\|1g7k]], [[2h5o\|2h5o]], [[2h5p\|2h5p]], [[2h5q\|2h5q]], [[2h5r\|2h5r]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1g7k\|1g7k]], [[2h5o\|2h5o]], [[2h5p\|2h5p]], [[2h5q\|2h5q]], [[2h5r\|2h5r]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">dRFP583 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=86600 ~~Discosoma sp.~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">dRFP583 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=86600 DISSP])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2h8q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2h8q OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2h8q RCSB], [http://www.ebi.ac.uk/pdbsum/2h8q PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2h8q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2h8q OCA], [http://pdbe.org/2h8q PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2h8q RCSB], [http://www.ebi.ac.uk/pdbsum/2h8q PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
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	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 2h8q" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 35:		Line 36:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Discosoma sp~~]]	+	[[Category: Dissp]]
	[[Category: Remington, S J]]		[[Category: Remington, S J]]
	[[Category: Yarbrough, C A]]		[[Category: Yarbrough, C A]]

OCA at 18:33, 24 December 2014

OCA — Wed, 24 Dec 2014 18:33:03 GMT

←Older revision		Revision as of 18:33, 24 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2h8q]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Discosoma_sp. Discosoma sp.]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2H8Q OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2H8Q FirstGlance]. <br>		<table><tr><td colspan='2'>[[2h8q]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Discosoma_sp. Discosoma sp.]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2H8Q OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2H8Q FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CRQ:[2-(3-CARBAMOYL-1-IMINO-PROPYL)-4-(4-HYDROXY-BENZYLIDENE)-5-OXO-4,5-DIHYDRO-IMIDAZOL-1-YL]-ACETIC+ACID'>CRQ</scene></td></tr>	+	</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CRQ:[2-(3-CARBAMOYL-1-IMINO-PROPYL)-4-(4-HYDROXY-BENZYLIDENE)-5-OXO-4,5-DIHYDRO-IMIDAZOL-1-YL]-ACETIC+ACID'>CRQ</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1g7k\|1g7k]], [[2h5o\|2h5o]], [[2h5p\|2h5p]], [[2h5q\|2h5q]], [[2h5r\|2h5r]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1g7k\|1g7k]], [[2h5o\|2h5o]], [[2h5p\|2h5p]], [[2h5q\|2h5q]], [[2h5r\|2h5r]]</td></tr>
-	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">dRFP583 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=86600 Discosoma sp.])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">dRFP583 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=86600 Discosoma sp.])</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2h8q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2h8q OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2h8q RCSB], [http://www.ebi.ac.uk/pdbsum/2h8q PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2h8q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2h8q OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2h8q RCSB], [http://www.ebi.ac.uk/pdbsum/2h8q PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/RFP_DISSP RFP_DISSP]] Thought to play a role in photoprotection of the coral's resident symbiont microalgae's photosystems from photoinhibition caused by high light levels found near the surface of coral reefs. In deeper water, the fluorescence may be to convert blue light into longer wavelengths more suitable for use in photosynthesis by the microalgal symbionts.<ref>PMID:10504696</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 33:		Line 35:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Discosoma sp.]]	+	[[Category: Discosoma sp]]
-	[[Category: Remington, S J.]]	+	[[Category: Remington, S J]]
-	[[Category: Yarbrough, C A.]]	+	[[Category: Yarbrough, C A]]
	[[Category: Beta barrel]]		[[Category: Beta barrel]]
	[[Category: Bioluminescence]]		[[Category: Bioluminescence]]

OCA at 05:14, 3 October 2014

OCA — Fri, 03 Oct 2014 05:14:22 GMT

←Older revision		Revision as of 05:14, 3 October 2014
Line 1:		Line 1:
-	[[~~Image:~~2h8q.~~png~~\|~~left~~\|200px]]	+	==Crystal Structure of a Redshifted Mutant (K83M) of the Red Fluorescent Protein dRFP583/dsRed==
		+	<StructureSection load='2h8q' size='340' side='right' caption='[[2h8q]], [[Resolution\|resolution]] 2.00Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[2h8q]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Discosoma_sp. Discosoma sp.]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2H8Q OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2H8Q FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CRQ:[2-(3-CARBAMOYL-1-IMINO-PROPYL)-4-(4-HYDROXY-BENZYLIDENE)-5-OXO-4,5-DIHYDRO-IMIDAZOL-1-YL]-ACETIC+ACID'>CRQ</scene></td></tr>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1g7k\|1g7k]], [[2h5o\|2h5o]], [[2h5p\|2h5p]], [[2h5q\|2h5q]], [[2h5r\|2h5r]]</td></tr>
		+	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">dRFP583 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=86600 Discosoma sp.])</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2h8q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2h8q OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2h8q RCSB], [http://www.ebi.ac.uk/pdbsum/2h8q PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/h8/2h8q_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	mFruits are second-generation monomeric red fluorescent proteins (mRFPs) that have improved brightness and photostability compared to the first-generation mRFP1. The emission and excitation maxima are distributed over the remarkably large ranges of about 550-650 and 540-590 nm, respectively; however, the variations in the spectra can be traced to a few key amino acids. Spectroscopic and atomic resolution crystallographic analyses of three representatives, mOrange, mStrawberry, and mCherry, reveal that different mechanisms operate to establish the excitation and emission maxima. Evidently, they all undergo the second oxidation step to produce an acylimine linkage in the polypeptide backbone. In comparison to the progenitor DsRed, direct covalent modification to this linkage (mOrange) and indirect modification of the chromophore environment (mStrawberry and mCherry) produce strong blue- and red-shifted variants. The blue shift of mOrange is induced by an unprecedented covalent modification of the protein backbone. The electron-density map indicates the formation of a third heterocycle, 2-hydroxy-dihydrooxazole, upon the reaction of Thr 66 Ogamma with the polypeptide backbone, which in turn reduces the conjugation of the carbonyl at position 65 with the rest of the chromophore. In mStrawberry and mCherry, the movement of charged Lys 70 and protonation of Glu 215 are proposed to modify the chromophore electron-density distribution, inducing the red shift. pH-dependent spectral shifts of mCherry and mStrawberry appear to result from the titration of Glu 215, although, for mStrawberry, partial cyclization of Thr 66 may contribute at high pH.

-	~~{{STRUCTURE_2h8q\| PDB=2h8q \| SCENE= }}~~	+	Novel chromophores and buried charges control color in mFruits.,Shu X, Shaner NC, Yarbrough CA, Tsien RY, Remington SJ Biochemistry. 2006 Aug 15;45(32):9639-47. PMID:16893165<ref>PMID:16893165</ref>

-	~~===Crystal Structure of~~ a ~~Redshifted Mutant (K83M)~~ of the ~~Red Fluorescent Protein dRFP583/dsRed===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_16893165}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[2h8q]] is a 4 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Discosoma_sp~~. ~~Discosoma sp.]. Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=2H8Q OCA].~~	+

	==See Also==		==See Also==
-	*[[Alyssa Marsico/Sandbox 1\|Alyssa Marsico/Sandbox 1]]
-	*[[Devon McCarthy/Sandbox 1\|Devon McCarthy/Sandbox 1]]
	*[[Green Fluorescent Protein\|Green Fluorescent Protein]]		*[[Green Fluorescent Protein\|Green Fluorescent Protein]]
-	*[[Sandbox104\|Sandbox104]]	+	== References ==
-	*[[User:Joanne Lau/Sandbox 5\|User:Joanne Lau/Sandbox 5]]	+	<references/>
-		+	__TOC__
-	==~~Reference~~==	+	</StructureSection>
-	<~~ref group="xtra">PMID:016893165<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Discosoma sp.]]		[[Category: Discosoma sp.]]
	[[Category: Remington, S J.]]		[[Category: Remington, S J.]]

OCA at 07:59, 20 October 2012

OCA — Sat, 20 Oct 2012 07:59:57 GMT

←Older revision		Revision as of 07:59, 20 October 2012
Line 8:		Line 8:

	==About this Structure==		==About this Structure==
-	[[2h8q]] is a 4 chain structure ~~of [[Alyssa Marsico/Sandbox 1]], [[Devon McCarthy/Sandbox 1]], [[Green Fluorescent Protein]], [[Sandbox104]] and [[User:Joanne Lau/Sandbox 5]]~~ with sequence from [http://en.wikipedia.org/wiki/Discosoma_sp. Discosoma sp.]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2H8Q OCA].	+	[[2h8q]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Discosoma_sp. Discosoma sp.]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2H8Q OCA].

	==See Also==		==See Also==

OCA: Protected "2h8q" [edit=sysop:move=sysop]

OCA — Wed, 25 Jul 2012 18:07:32 GMT

Protected "2h8q" [edit=sysop:move=sysop]

←Older revision

Revision as of 18:07, 25 July 2012

OCA at 18:07, 25 July 2012

OCA — Wed, 25 Jul 2012 18:07:31 GMT

←Older revision		Revision as of 18:07, 25 July 2012
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-	{{Seed}}
	[[Image:2h8q.png\|left\|200px]]		[[Image:2h8q.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_2h8q", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
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	{{STRUCTURE_2h8q\| PDB=2h8q \| SCENE= }}		{{STRUCTURE_2h8q\| PDB=2h8q \| SCENE= }}

	===Crystal Structure of a Redshifted Mutant (K83M) of the Red Fluorescent Protein dRFP583/dsRed===		===Crystal Structure of a Redshifted Mutant (K83M) of the Red Fluorescent Protein dRFP583/dsRed===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_16893165}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 16893165 is the PubMed ID number.
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	{{ABSTRACT_PUBMED_16893165}}		{{ABSTRACT_PUBMED_16893165}}

	==About this Structure==		==About this Structure==
-	~~2H8Q~~ is a 4 ~~chains~~ structure of ~~sequences~~ from [http://en.wikipedia.org/wiki/Discosoma_sp. Discosoma sp.]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2H8Q OCA].	+	[[2h8q]] is a 4 chain structure of [[Alyssa Marsico/Sandbox 1]], [[Devon McCarthy/Sandbox 1]], [[Green Fluorescent Protein]], [[Sandbox104]] and [[User:Joanne Lau/Sandbox 5]] with sequence from [http://en.wikipedia.org/wiki/Discosoma_sp. Discosoma sp.]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2H8Q OCA].
		+
		+	==See Also==
		+	*[[Alyssa Marsico/Sandbox 1\|Alyssa Marsico/Sandbox 1]]
		+	*[[Devon McCarthy/Sandbox 1\|Devon McCarthy/Sandbox 1]]
		+	*[[Green Fluorescent Protein\|Green Fluorescent Protein]]
		+	*[[Sandbox104\|Sandbox104]]
		+	*[[User:Joanne Lau/Sandbox 5\|User:Joanne Lau/Sandbox 5]]

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~16893165~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:016893165</ref><references group="xtra"/>
	[[Category: Discosoma sp.]]		[[Category: Discosoma sp.]]
	[[Category: Remington, S J.]]		[[Category: Remington, S J.]]
Line 34:		Line 29:
	[[Category: Luminescent protein]]		[[Category: Luminescent protein]]
	[[Category: Red shift]]		[[Category: Red shift]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jul 8 12:50:18 2009''